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1.
Antibody seroprevalences against peste des petits ruminants (PPR) virus in camels, cattle, goats and sheep in Ethiopia 总被引:1,自引:0,他引:1
Abraham G Sintayehu A Libeau G Albina E Roger F Laekemariam Y Abayneh D Awoke KM 《Preventive veterinary medicine》2005,70(1-2):51-57
A questionnaire-survey data indicated that 26% of 276 farmers reported the presence of respiratory disease in their herds in 2001. The incidence was perceived as "high" in small ruminants and camels, but as "low" in cattle. Simultaneously, 2815 serum samples from camels (n=628), cattle (n=910), goats (n=442) and sheep (n=835) were tested. The peste des petits ruminants (PPR) antibody seroprevalence was 3% in camels, 9% in cattle, 9% in goats and 13% in sheep. The highest locality-specific seroprevalences were: camels 10%, cattle 16%, goats 22% and sheep 23%. The animals had not been vaccinated against rinderpest or PPR. Antibody seroprevalences detected in camels, cattle, goats and sheep confirmed natural transmission of PPR virus under field conditions. 相似文献
2.
One hundred and ninety-five goat and 67 sheep sera collected from various parts of southern Nigeria were screened for neutralising antibodies to both the peste des petits ruminants (PPR) and rinderpest viruses. Neutralising antibodies against both viruses were found in the sheep and goat sera examined. Parallel titration of samples which neutralised both viruses indicated a primary infection with the PPR virus (PPRV). However, some samples which failed to neutralise PPRV neutralised the rinderpest virus (RV) indicating RV activity in sheep and goats in Nigeria. These findings are discussed in relation to the diagnosis of PPRV infection and the recent reappearance of bovine rinderpest in Nigeria. 相似文献
3.
A Diallo W P Taylor P C Lefèvre A Provost 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1989,42(3):311-319
Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants frequently associated with severe mortality in these hosts. In countries where it occurs, PPR represents an important constraint to the improved productivity of sheep and goats. Until now the only way to combat this plague has been the use of heterologous rinderpest vaccine; all attempts to develop a homologous vaccine have ended in failure. The present communication describes the attenuation of the Nigerian strain PPRV Nig 75/1 by serial passage in Vero cells. The avirulent virus obtained has the same characteristics as Plowright and Ferris' rinderpest vaccine. The virus is advanced as a potential homologous vaccine against PPR. 相似文献
4.
The production of peste des petits ruminants hyperimmune sera in rabbits and their application in virus diagnosis 总被引:4,自引:0,他引:4
T U Obi K C McCullough W P Taylor 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1990,37(5):345-352
Hyperimmune sera were produced by serial inoculation of rabbits with Vero cell-adapted, sucrose gradient-purified Nigerian peste des petits ruminants virus (PPRV) isolate. Two antisera produced, neutralized the homologous PPRV but not the heterologous rinderpest Kabette "O" virus. The antisera gave strong precipitin lines with purified PPRV antigens and were used to detect PPRV and rinderpest virus antigens from ante-mortem secretions and post-mortem tissue homogenates from PPR and rinderpest virus infected goats and cattle by the agar gel precipitation tests (AGPT). The hyperimmune sera gave good titration curves with both purified Nigerian goat and the United Arab Emirate wildlife PPRV isolates in the indirect enzyme linked immunosorbent assay (ELISA). Results of indirect ELISA showed that although there were some cross reactions with the rinderpest, canine-distemper and measles viruses, at 1:100 dilution, the antisera would give a positive signal with only the homologous PPR virus. 相似文献
5.
6.
Seroprevalence of Peste des petits ruminants in cattle and buffaloes from Southern Peninsular India 总被引:1,自引:1,他引:0
Balamurugan V Krishnamoorthy P Veeregowda BM Sen A Rajak KK Bhanuprakash V Gajendragad MR Prabhudas K 《Tropical animal health and production》2012,44(2):301-306
This study describes seroprevalence of Peste des petits ruminants (PPR) in cattle and buffaloes carried out during the period 2009–2010 using the randomly collected serum samples from different
parts of Southern peninsular India. The report presents the results of PPR virus (PPRV)—specific antibodies in situations
where either the subclinical or inapparent or non-lethal infection was there in cattle and buffaloes. A total of 2,548 serum
samples [cattle = 1,158, buffaloes = 1,001, sheep = 303 and goat = 86] were collected and screened for PPRV antibodies by
using a PPR monoclonal antibody-based competitive ELISA kit. Analysis of 2,159 serum samples indicates an overall 4.58% prevalence
of PPRV antibody in cattle and buffaloes. The presence of PPRV-specific antibodies demonstrates that cattle and buffaloes
are exposed to PPR infection naturally, and the transmission mode may be direct or indirect. Further, it implies the importance
of bovines as subclinical hosts for the virus besides widespread presence of the disease in sheep and goats in the country. 相似文献
7.
Emmanuel Senyael Swai A. Kapaga F. Kivaria D. Tinuga G. Joshua P. Sanka 《Veterinary research communications》2009,33(8):927-936
Despite the widespread prevalence of infection with Peste des petits ruminants virus (PPRV) in goats and sheep industry in
Asia and sub-Saharan Africa, there have been few, if any, structured population-based studies examining the epidemiology of
this infection in Tanzania. In this study, we investigated the seroprevalence, and risk factors, of Peste des petitis ruminants(PPR)
in sheep and goat flocks from seven different geographical administration authorities (Ngorongoro, Monduli, Longido, Karatu,
Mbulu, Siha and Simanjiro) located in Northern Tanzania. Serum samples from 657 and 892 sheep and goats, respectively, corresponding
to 91 sheep/goat flocks and 43 villages were collected. Competitive enzyme linked immunosorbent assay (c-ELISA) was used to
detect the presence of antibodies in the serum against PPRV. Chi-square analysis and multivariable logistic regression model
were used to identify risk factors for PPRV seropositivity. Findings suggested that the sero-positive cases were significantly
higher in goats than in sheep (49.5% versus 39.8%; P = 0.002). The overall seroprevalence of PPRV infection in small ruminants
was 45.8%. Highest seroprevalence (42.6–88.02%) was observed in Mbulu, Siha, Longido, Ngorongoro districts, while antibodies
less than 40% to none were found in serum from Monduli, Karatu and Simanjiro, respectively. These findings confirm natural
transmission of PPRV under field condition for the first time in Tanzania. Results may be correlated with variations in the
sheep and goat husbandry practices within different geographic localities, the uncontrolled movement of animals, the levels
of natural immunity and the sharing of grazing field amongst agro and pastoralists. 相似文献
8.
Zahur AB Ullah A Hussain M Irshad H Hameed A Jahangir M Farooq MS 《Preventive veterinary medicine》2011,102(1):87-92
A sero survey was conducted during 2005-2006 to estimate the sero prevalence of PPR in the small ruminant population of Pakistan. A total of 2798 samples were collected including goats (1979) and sheep (819) from villages in 27 randomly selected districts. These were tested by cELISA for PPRV and true prevalence estimates were calculated by Rogan and Gladen estimator. Overall, 1273 (45.5%) were found positive; 980 (49.5%) of 1979 samples from goats and 293 (35.8%) of 819 serum samples from sheep were positive. The true sero-prevalence of PPR was estimated to be 48.5% (95% CI, 46.6-50.3), and 52.9% (95% CI, 50.7-55.1) and 37.7 (95% CI, 34.4-41.0) for goats and sheep, respectively. PPR virus is widely distributed all across Pakistan and has become an endemic infection of small ruminants. Since it is one of the leading causes of morbidity and mortality in small ruminants, it poses a serious threat to food security and the rural economy in Pakistan. 相似文献
9.
10.
Khan HA Siddique M Arshad M Abubakar M Akhtar M Arshad MJ Ashraf M 《Tropical animal health and production》2009,41(4):427-430
A total of 70 sheep and 330 goats were selected randomly. All the animals were kept under same housing and management conditions.
Serum samples were collected from all the animals and tested for the presence of antibodies against Peste des petits ruminants
(PPR) virus using competitive ELISA (cELISA). All the animals were found negative showing percentage inhibition (PI) values
<50. The animals were vaccinated against PPR with Nig/75/1 strain vaccine of PPR Serum samples were collected from randomly
selected 12 sheep and 30 goats at 10, 30 and 45 days post-vaccination. The samples were subjected to cELISA to determine the
presence of antibodies against PPRV. The samples with PI >50 were considered as sero-positive. The sheep found positive at
10, 30 and 45 days post-vaccination were 1(8.3%), 7(58.3%) and 12(100%) respectively. In case of goats 3(10.0%), 29(96.6%)
and 27(90.0%) animals gave positive results at 10, 30 and 45 days post-vaccination respectively. Mean PI values in sheep at
10, 30 and 45 days post-vaccination were recorded as 37, 65 and 91 respectively, whereas in goats these values were 43, 78
and 86 respectively. 相似文献
11.
K. S. Intisar Y. H. Ali M. A. Haj M. A. T. Sahar M. M. Shaza A. M. Baraa O. M. Ishag Y. M. Nouri K. M. Taha E. M. Nada A. M. Ahmed A. I. Khalafalla G. Libeau A. Diallo 《Tropical animal health and production》2017,49(4):747-754
The existence of peste des petits ruminants (PPR) in domestic ruminants and camels in Sudan during 2008–2012 was investigated. Lung tissues and serum samples were randomly collected from sheep, goats, cattle, and camels at different areas of Sudan. A total of 12,384 serum samples were collected from clinically healthy 7413 sheep, 1988 camels, 1501 cattle, 1459 goats, and 23 gazelles at different areas in the Sudan. They were examined for PPR antibodies using competitive ELISA (cELISA). The overall detected seroprevalence of PPR in tested sera was 49.4%; seroprevalence values within species were 67.1, 48.2, 25.8, 2.1, and 21.7% in sheep, goat, cattle, camels, and gazelles, respectively. The highest seroprevalence (68.1%) was observed in sera collected from Darfur states, then the central states (54.3%). A total of 1276 lung tissue samples (623 sheep, 324 cattle, 220 camels, and 109 goats) were collected. The majority of lung samples were collected from clinically healthy animals that showed lesions on PM in slaughterhouses (95%) and during PPR outbreaks; samples were tested for PPR antigen using immunocapture ELISA (IcELISA). PPR antigen was detected in 233 out of the 1276 tested samples (18.3%). Positive results were observed in samples collected from clinically healthy and diseased animals. The observed prevalence values in each species were 33.6, 21.1, 15.4, and 12.3% in camel, goat, sheep, and cattle, respectively. PPR antigen was detected in samples from different areas; however, the highest prevalence (63.9%) was found in samples collected from the eastern states, then Khartoum state (28%). Trials for virus isolation were done in different cell cultures. Out of 30 IcELISA-positive samples inoculated in primary bovine and ovine kidney cells, Vero cells, the PPR virus was successfully isolated from 15 (eight sheep, five camels, and two goats) samples in the three cell culture types. Using RT-PCR, PPRV nucleic acid was detected in all 25 IcELISA-positive tested samples. 相似文献
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13.
Development of a monoclonal antibody based competitive-ELISA for detection and titration of antibodies to peste des petits ruminants (PPR) virus 总被引:6,自引:0,他引:6
Peste des petits ruminants (PPR) is an acute febrile, viral, disease of small ruminants with great economic importance. A competitive-ELISA (c-ELISA) test was developed for detection of antibodies to PPR virus in the sera samples of goats and sheep. The test uses monoclonal antibody to a neutralizing epitope of haemagglutinin protein of the virus. Based on the distribution of known negative sera samples (n=933) in respect of PPR virus antibodies in the test, a cut-off value was set as 38%. This value was the result of mean of negative population added with two times the standard deviations. A total of 1668 sera samples from goat and sheep and 32 sera from cattle were screened by c-ELISA and virus neutralization test (VNT). Efficacy of c-ELISA compared very well with VNT having high relative specificity (98.4%) and sensitivity (92.4%). The sensitivity of c-ELISA for PPR sero-surveillance could further be increased (95.4%), if the target population is non-vaccinated. c-ELISA test correlated well with VNT (r=0.845) for end-point titration of PPR virus antibody in 64 goat sera samples. It could clearly separate infected population from uninfected in field sera. Using c-ELISA test paired sera samples from 13 goats provided a clear diagnosis of PPR virus infection. Furthermore, antibodies to PPR virus could be successfully detected during 1 year after vaccination in four goats inoculated with an experimental PPR vaccine. Findings suggest that the c-ELISA test developed can easily replace VNT for sero-surveillance, sero-monitoring, diagnosis from paired sera samples and end-point titration of PPR virus antibodies. 相似文献
14.
Nussieba A. Osman A. S. Ali Mahasin E. A/Rahman M. A. Fadol 《Tropical animal health and production》2009,41(7):1449-1453
Counter immnuo-electrophoresis (CIEP) and Competitive ELISA (C-ELISA) tests were employed for seroprevalence of Peste des Petits Ruminants (PPR) infection in Sudan. The result of both tests showed high prevalence of PPRV antibodies in sheep and goats sera collected from six different regions of Sudan. Of the 519 serum samples examined for the presence of PPRV antibodies 307(59.15%) were positive by CIEP while 263(50.67%) were positive by C-ELISA. CIEP technique was shown to be more sensitive than C-ELISA technique for detection of PPRV antibodies (Kappa statistics 0.259). C-ELISA allowed rapid, simple, specific, sensitive and differential sero-diagnosis of PPRV and RPV in sheep, goats and cattle. CIEP is, unlike competitive ELISA, is group-specific test and can not differentiate between PPR and RP infections. Despite its low specificity CIEP can be a useful indicative screening test for PPRV antibodies in flocks that neither been vaccinated nor otherwise exposed to PPR or RP virus. Results obtained suggest that CIEP, like the HI test, could be a useful screening test where it is not possible to use C-ELISA. 相似文献
15.
Chunsheng Yin Weiye Chen Qianqian Hu Zhiyuan Wen Xijun Wang Jinying Ge Qianqian Yin Haibing Zhi Chun Xia Zhigao Bu 《Veterinary research》2014,45(1):62
Peste des petits ruminants (PPR) and foot-and-mouth disease (FMD) are both highly contagious diseases of small domestic and wild ruminants caused by the PPR virus (PPRV) and the FMD virus (FMDV). In this study, a recombinant PPRV expressing the FMDV VP1 gene (rPPRV/VP1) was generated and FMDV VP1 expression did not impair replication of the recombinant virus in vitro and immunogenicity in inducing neutralizing antibody against PPR in goats. Vaccination with one dose of rPPRV/VP1 induced FMDV neutralizing antibody in goats and protected them from challenge with virulent FMDV. Our results suggest that the recombinant PPRV expressing the FMDV VP1 protein is a potential dual live vectored vaccine against PPRV and FMDV. 相似文献
16.
Hammouchi M Loutfi C Sebbar G Touil N Chaffai N Batten C Harif B Oura C El Harrak M 《Veterinary microbiology》2012,160(1-2):240-244
Peste des petits ruminants virus (PPRV) recently caused a serious outbreak of disease in Moroccan sheep and goats. Alpine goats were highly susceptible to PPRV with mortality rates approaching 100%, as opposed to local breeds of sheep which were less susceptible to the disease. The relative susceptibility of alpine goats was investigated through an experimental infection study with the Moroccan strain of PPRV. Severe clinical signs were observed in the alpine goats with virus being excreted through ocular, nasal and oral routes. No difference in the severity of the disease in goats was observed with different inoculation routes and transmission of the virus by direct contact was confirmed. This study confirmed the susceptibility of the alpine goat to PPRV infection and describes a challenge protocol that effectively and consistently reproduced severe clinical signs of PPR in experimentally infected goats. 相似文献
17.
A competitive ELISA (C-ELISA) using monoclonal antibodies (mAbs) which bind to the nucleo-protein (NP) of rinderpest virus (RPV) for detection of RPV antibodies in cattle and small ruminant sera is described. Unlike virus neutralisation test (VNT), this test using mAb IVB2-4, can detect specific RPV antibodies without showing a cross-reaction with antibodies to peste-des-petits ruminants-virus (PPRV); by contrast, when mAb VE4-1 is used the test detects both RPV and PPRV antibodies, including low levels of antibodies that can be found in sera containing maternal antibodies. Although antibodies to the PPRV 75-1 strain are also detected with mAb 51-5-6, the test is suitable for assessing the immune status of cattle against the Rinderpest Old Kabete (RBOK) strain. The results from a panel of sera with a known status of vaccination provide evidence for a highly significant correlation between C-ELISA and VNT. This test may be a useful tool for a standardized and accurate determination of the immunity status of both cattle and small ruminants. 相似文献
18.
小反刍兽疫分子生物学研究进展 总被引:2,自引:1,他引:1
小反刍兽疫(peste des petits ruminants,PPR)是由小反刍兽疫病毒(peste des petits ruminants virus,PPRV)引起的一种急性、烈性、接触性传染病。山羊高度易感;牛、猪等动物也可以感染带毒,野生动物偶有发生。作者主要介绍了小反刍兽疫病毒各基因结构特点,6种结构蛋白的功能,以及小反刍兽疫的诊断技术等方面的最新研究进展。 相似文献
19.
Diallo A 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2006,53(Z1):11-13
Peste des petits ruminants (PPR) is a contagious and often fatal viral disease of sheep and goats and also wild small ruminants. The PPR virus is distinct from but closely related to rinderpest virus and both belong to the morbivillivirus genus within the family Paramyxoviridae. PPR is a contagious transboundary disease with a significant impact on rural poor farmers. Its control should therefore be considered in programs that aim at alleviating poverty in developing countries. 相似文献
20.
小反刍兽疫病毒(PPRV)是副黏病毒科(Paramyxoviridae)麻疹病毒属(Morbolivirus)的成员,主要感染山羊、绵羊等小反刍动物,引起一种高度接触性病毒性传染病。小反刍兽疫为一种重大的外来性疾病,2007年在中国西藏自治区日土县首次发生。自2013年末以来中国新疆、青海、甘肃、宁夏、内蒙、湖南、辽宁等地频繁暴发小反兽疫疫情,给中国的畜牧业带来了巨大的损失,引起极大的重视。为更好的分析小反刍兽疫的病原特性及采取有效的防控措施,文章对小反刍兽疫的病原学及疫苗研究进展进行论述。 相似文献