Performance of Spelt (Triticum spelta L.) and Wheat (Triticum aestivum L.) at two Different Seeding Rates and Nitrogen Levels under Contrasting Environmental Conditions

The aim of this investigation was to obtain information about differences between spelt and wheat in relation to their performance under low-input conditions. Two spelt varieties ( Oberkulmer, Hercule ) and two wheat varieties ( Anna, Iend ) were grown at two different seeding rates (S1 = 200; S2 = 400 kernels/m²) and two nitrogen levels (Nl = 80 kg N/ha; N2 = 110 kg N/ha) at two contrasting locations (Muri; altitude 459 m asl; Oberwallestalden: altitude 1011 m asl). No growth regulators and fungicides were applied.
The overall mean for the "husked" yield (grains and glumes) of spelt was not significantly different from the grain yield of wheat. At the higher site Oberwallestalden however, the "husked" yield of spelt was 10.5 % higher than the grain yield of wheat. Although the number of ears per m² was higher at S2 than at S1, spelt and wheat produced a similar yield at both seeding rates (spelt: S1 = 96 % of S2; wheat: S1 = 95 % of S2) due to the higher single ear weight at S1.
The reaction of spelt and wheat to nitrogen was similar. The yield was 10 % higher at N2 than at Nl for both species. The performance of spelt under low-input conditions was better than for wheat in marginal areas for cereal production only.     

Nitrate Assimilation and Nitrogen Harvest in Uniculm 'Gigas' Wheat (Triticum aestivum L.)

Contribution of the upper laminae designated as 7, 8 and 9 (flag), to the total reduced nitrogen was determined by two different methods; integration of nitrate reductase activity over the entire growing season of individual laminae, and laminae removal at ear emergence. The values obtained by these methods were: 20, 14, and 12 %, and 23, 15, 12 %, respectively. The in vivo nitrate reductase activity was relatively higher in the lamina 9, which contributed an extra 8 % towards total reduced nitrogen when the other laminae were removed from the shoot. No such compensatory response was observed when either lamina 7 or 8 was retained.     

花后灌水对小麦籽粒品质性状及产量的影响

在池栽防雨条件下,研究了小麦花后不同灌水时期、不同灌水次数对籽粒产量及品质性状的影响。结果表明,在花前限量灌水条件下（135 mm）,花后灌水（45～90 mm）可显著提高小麦籽粒产量及蛋白质产量;虽然多数品质性状在花后不灌水（CK）条件下获得最大值,但灌1水未引起品质性状的明显变化;随灌水次数增加,各品质性状变劣     

Die Ertragsbildung von Dinkel (Triticum spelta L.) und Weizen (Triticum aestivum L.) unter verschiedenen Umweltbedingungen im Freiland

Dry Matter Production of Spelt ( Triticum spelta L.) and Wheat ( Triticum aestivum L.) under Different Environmental Conditions in the Field
The primary aim of our work was to obtain information regarding the germination characteristics of spelt under different environmental conditions in field experiments, and to trace possible differences between spelt and wheat, with regard to crop production. For the present study two spelt varieties ( Ostro/Rouquin) and two wheat varieties ( Arina/Probus) were used. In addition the spelt cultivars Altgold, Oberkulmer and Hercule were included in some of the experiments. To obtain information on the influence of the glumes, spelt was planted in the hulled (SP +) and dehulled (SP-) form.
Field emergence of spelt SP- was up to 40 % lower than that of SP+, Under water logged soil, the percentage of germination was higher for spelt SP+ than for wheat.
Spelt produced more tillers per plant than wheat. However, tiller reduction was also higher for spelt so finally both species had similar numbers of ears per plant. Plants with a high tiller production might be more flexible in response to unfavourable growing conditions.
Grain yield of spelt and wheat was developed in different ways. Spelt produced fewer but larger kernels per ear, whereas wheat produced more but smaller kernels. In 1986 grain yield was up to 20 % higher in wheat than in spelt. In 1987, an unfavourable year for cereal crop production in marginal areas, grain yield was higher for spelt than for wheat. For the farmer, the "husked" form of spelt is economically important. This was equal or higher than the grain yield of wheat in both years.
The high germination rate under unfavourable conditions, in combination with a high tillering capacity and the production of large kernels appear to be the major reason for the stable yielding ability of spelt.     

Agronomic and Physiological Study of Cold and Flooding Tolerance of Spelt (Triticum spelta L.) and Wheat (Triticum aestivum L.)

Flooding of soil occurs all over the world in areas of crop production. The resulting lack of available oxygen in the soil can hinder the establishment of field crops such as wheat ( Triticum aestivum L.) and thus lead to a decrease in yield. The current study compares the tolerance of spelt ( Triticum spelta L.) and wheat to wet and cold stress during germination and early growth. Two specific traits seem to be related to the superior early flooding tolerance of spelt: (1) fast growth of the coleoptile, which reaches the soil surface rapidly, thus reducing the time span of hypoxia sensitivity, independent of temperatures between 10 and 20 °C; (2) the improved physiological adaptation for coleoptile growth under hypoxia is probably due to lower oxygen consumption between germination and emergence. Because of this high level of flooding tolerance, spelt would also be a good source for breeding for stress tolerance in wheat. These findings will help to detect quantitative trait loci (QTL) for characters such as growth rate, flooding tolerance and oxygen consumption in future studies. A marker-assisted introgression of spelt into wheat is necessary to avoid combination with undesirable agronomic traits of spelt.     

小麦株高近等基因系的RAPD标记研究

采用ＲＡＰＤ技术,以４个小麦株市基因近等基因系（分别含有rht、Ｒht1、Rht2、Rht3)为材料,对２９６个单一随机引物（１０个核苷酸）进行了筛选。发现２５个引我的扩增产物的近等基因系间表现出特异性,在６次重复试验中,有１８个引物的特异护增片段不能重复,６个引物可以重复２－３资,唯有ＯＰＡＭ０１在全部试验中均能稳定重复,其特异扩增版段ＯＰＡＭ０１１８６０可以作为rht基因的ＲＡＰＤ标记。     

小麦供锌状况对叶片结构及叶绿体超微结构的影响

锌缺乏或过量使小麦叶肉细胞变小,多环复式细胞显著减少。缺锌植株的叶绿体中基粒垛数少,基粒垛叠片层少,基质片层少,部分片层膨胀;叶绿体内包含较大的液泡和淀粉“泡”;叶绿体被膜模糊,甚至消失.锌过量则使小麦的叶绿体变小,基粒和基质片层明显减少,部分片层膨胀,亲锇颗粒多;在叶绿体周围线粒体出现较多.     

普通小麦D染色体组微卫星分子标记遗传差异研究

本研究采用微卫星(SSR)分子标记技术, 对我国不同生态区的6个春小麦品种(系)及北方冬麦区的17个冬小麦品种(系)D染色体组的遗传多样性进行了分析. 结果显示, 23个微卫星引物在23份材料间共扩增出65个等位基因, 平均每个引物为2.9个. 分析发现, 冬小麦群体内检测到的等位基因数(60个)及平均遗传距离(0.4504)明显高于春小麦(48     

Yield, Grain Weight and Height Relationships in Two Random Samples of Early Semi-Dwarf Genotypes of Spring Wheat (Triticum aestivum L.)

The objective of this work is to study the association between the genotypic differences in mean grain weight and grain yield, the relationship of yield and grain weight with culm length, and the differences between the effects of the Rht1 and Rht2 dwarfing alleles on these characters. Yield, grain weight, grains m^-2 and culm length were evaluated in two random samples of 19 semi-dwarf early lines, selected in F₅ and tested in F₂, from two different crosses between cultivars differing in the Rht allele controlling their semi-dwarf ness. The lines of each cross were tested in 4-replicated field trials at two different sites. Phenotypic, genotypic, partial and multiple correlations as well as hertability estimates were computed. In each cross the lines differed significantly in their mean values of all the characters tested. In both crosses there was no apparent association between grain weight and grain yield. It therefore seems that in wheat of the type investigated in this study, grain yield and grain weight are independent controlled and that high grain yield is not restricted to any particular range of mean grain weight. A rather high positive correlation between culm length and grain weight was found in one cross but not in the other indicating the dependence of this relationship on the genetic background. In both crosses no significant differences were found between the performance of the lines carrying the Rht1 dwarfing allele and those carrying the Rht2 allele.     

The Influence of Different Nitrogen Levels and Seeding Rates on the Dry Matter Production and Nitrogen Uptake of Spelt (Triticum spelta L.) and Wheat (Triticum aestivum L.) under Field Conditions

Dry matter production of two different spelt (Oberkulmer, Hercule) and wheat varieties (Arina, Iena) were investigated at two different seeding rates (S1 = 200 grains/m²; S2 = 400 grains/m²) and two nitrogen levels (N1 = 80 kg N/ha; N2 = 110 kg N/ha). The plot experiments were carried out at two contrasting locations (Muri: altitude 459 m asl); Oberwallestalden: altitude 1011 m asl) over three years (1988–1990). In addition nitrogen uptake and the photosynthetic rate of flag leaves was measured. Neither growth regulators nor fungicides were applied. The average grain yield of spelt was 25 % lower than that of wheat (32 % at Muri, 18 % at Oberwallestalden). At the reduced seeding rate (S1) grain weight and grain number per ear was increased by 33 % and 31 %, respectively as compared to the normal seeding rate (S2). The increase of the grain weight and the grain number per ear was larger for the two varieties of spelt (47 % and 42 %, respectively) than for the wheat varieties (23 % and 22 %, respectively). The photosynthetic rate of the flag leaf of spelt and wheat was not significantly different, at the two growth stages measured (anthesis, anthesis + 23 days). Nitrogen yield in the above ground biomass (g N/m²) was not significantly different between spelt and wheat, neither at the beginning of stem elongation, nor at anthesis. At anthesis the nitrogen yield at the reduced nitrogen level (N1) was 16 % and 13 % lower than at the higher level (N2) for spelt and wheat respectively. A higher nitrogen efficiency of spelt under low input conditions was not apparent. Therefore it was concluded that under low input conditions, spelt is not more efficient in dry matter production than wheat. By comparing the “husked” yield of spelt (grains + glumes; representing the trade form) with the grain yield of wheat, spelt is higher yielding than wheat but only at marginal areas of cereal production.     

Irrigation and Nutrient Effects on Growth and Water–Yield Relationship of Wheat (Triticum aestivum L.) in Central India

Increasing production of wheat from a limited water supply can result from efficient irrigation and nutrient management. A 3‐year field experiment was conducted at the Indian Institute of Soil Science, Bhopal, to study the growth, yield, seasonal evapotranspiration (ET) and water use efficiency (WUE), and the water–yield relationship of wheat in a soybean–wheat cropping system on vertisols. Three levels of irrigation, viz. I₀, no post‐sowing irrigation; I₁, two irrigations [crown root initiation (CRI) and flowering stage]; and I₂, three irrigations (CRI, maximum tillering and flowering stage) and three nutrient management treatments, viz. F₀, control (without fertilizer/manure); F₁, 100 % NPK (100–21.5–24.9 kg ha^?1); and F₂, 100 % NPK + farmyard manure (FYM‐10 t ha^?1) were tested in a split‐plot design with three replication. It has been established (through anova ) that the year effect was rather negligible and the interaction effects of irrigation and nutrient management on the growth parameters, ET, yield components, yield and WUE were significant. Plant height, progressive leaf area index, dry matter accumulation and crop growth rate were higher in I₂F₂, and I₂F₁ and I₁F₂ were statistically at par. The seasonal ET increased significantly with the increase in water supply in every nutrient treatment and it was highest in I₂F₂ and lowest in I₀F₀. The highest grain yield was obtained in I₂F₂; and a similar yield was recorded in I₃F₁ and I₂F₂. This shows a strong interaction effect between irrigation and nutrients. Yield components, viz. number of ears m^?2, number of grains ear^?1 and 1000‐grain weight were significant. The higher number of ears m^?2 containing greater number of grains with relatively heavier weights appeared to have contributed to the higher yield in I₁F₂, I₂F₁ and I₂F₂. The highest WUE obtained in I₀F₂ did not correspond to the highest yield and maximum ET, but a WUE of 10.43 kg ha^?1 mm^?1 in the I₂F₂ combination corresponded with the highest yield and the seasonal ET requirement was 391.8, which was 137 % greater than the water use at maximum WUE. The ET–grain yield relationship was linear, with a lowest regression slope (i.e. marginal WUE) and elasticity of water production (E_wp) in F₀ and a considerably higher slope and E_wp in F₁ and F₂. As the E_wp is positive and close to one in 100 % NPK treatment, the scope of improving WUE and yield with only inorganic fertilizer is very little, and relatively greater scope exists in the integrated management of organic manure and inorganic fertilizer. The results suggest that integrated nutrient management (100 % NPK + FYM) in conjunction with three irrigations maximized yield of wheat with concomitant improvement in ET and WUE under limited water availability.     

小麦Cyp450基因的电子克隆与生物信息学分析

为获得对小麦Cyp450基因及其编码蛋白质的理化性质与结构特性等,利用电子克隆方法克隆了一个新的小麦Cyp450基因,并对其编码的蛋白质进行了生物信息学分析。结果表明,该基因全长2155 bp,编码511个氨基酸;该蛋白序列的N端存在一个显著信号肽序列,属于CypX超家族,该蛋白定位于细胞质中,属于分泌途径中的信号肽蛋白,含有多个不同的磷酸化位点;二级结构以α螺旋（占48.14%）和无规则卷曲为主（占33.86%）,存在5个不稳定区,在85-511位处形成一个较大的球形蛋白域。该蛋白与大麦、二穗短柄草、水稻和高粱的同源Cyp450蛋白相似性较高,进化距离较近。获得了一个新的小麦细胞色素P450基因,并分析了其编码蛋白质的序列特性,为进一步实验克隆和研究其功能提供了理论基础。     

Effect of Split Nitrogen Application on Growth and Yield of Wheat (T. aestivum L.) Genotypes with Different N-Assimilation Potential

A field experiment was conducted with different nitrogen regimes to assess the growth and yield performance of wheat genotypes which differ in nitrate assimilation potential. Genotypic differences in biomass accumulation were observed at different growth stages. The nitrogen treatment had little effect on biomass accumulation at early stages of growth, while at later stages of growth there was enhanced biomass accumulation when N was applied in more than two splits. On an average, genotypes with high nitrate reductase activity (the 'HNR' genotypes) accumulated 14.2 % more biomass than the genotypes with low nitrate reductase activity ('LNR' genotypes) when an extra dose (40 kg N ha⁻¹) of nitrogen was given at the time of anthesis. The application of nitrogen in more than two splits increased grain yield of both 'HNR' and 'LNR' genotypes mainly by increasing grain weight per ear. The application of an extra dose of nitrogen (40 kg N ha⁻¹) at the time of anthesis increased grain yield of 'HNR' genotypes by 38.5 % as compared to 'LNR' genotypes.     

花药培养在小麦耐盐育种上的应用研究

在小麦花药培养中，采用加入一定浓度ＮａＣｌ（０．１％、０．３％、０．５％）的筛选培养基，用恒定浓度筛选法和逐渐增加浓度筛选法，可筛选出耐盐变异体，获得耐盐花培株系。两种筛选方法比较，逐渐增加浓度筛选法可以明显提高筛选效率。经５个有性世代的鉴定表明，耐盐变异体的耐盐特性可以稳定遗传给后代。经筛选的花培株系中，耐盐特性能够稳定遗传的株系比例约占１／４。在育种实践中，经多点试验鉴定，已选育出一批有实用价     

Distribution and Origin of Hybrid Necrosis Genes in German Winter Wheat (Triticum aestivum L.) Cultivars

Hybrid necrosis in Triticum is known to be caused by the interaction of two complementary dominant genes. In the present paper, the genotypes for hybrid necrosis of 64 winter wheat cultivars are presented. 41 cultivars were found to possess the Ne₂ necrosis gene, whereas 23 cultivars were non-carriers. The Ne₁ gene was not found in any of the cultivars analyzed.     

Joint Selection for High Yield and Low Sensitivity in Winter Wheat (Triticum aestivum L.)

Early generation yield trials of families from a set of winter wheat crosses were grown in three contrasting environments chosen to represent differences in two important macro-environmental variables affecting the wheat, crop in Eastern England. Joint selection for high yield and low sensitivity to these macro-environmental variables over two seasons using the procedure of Brumpton et al. (1977) resulted in a group of genotypes which exhibited a much wider yield range than would have resulted from selecting for yield in each -environment separately. In spite of this, the top performing lines an yield trials grown in each of the environments during the next season exhibited good yield and stability over all three environments, demonstrating the feasibility of such a method for winter wheat breeding.     

施用不同种类硫肥对豫麦49产量和品质的影响

于2000－2002年在土壤有效硫含量为15.8 mg.kg^-1的大田试验条件下,不同种类硫肥以60 kg.hm^-2纯S施入,研究了施用不同硫肥对冬小麦产量及品质的影响。结果表明,施用硫肥处理使小麦在籽粒灌浆中后期明显保持较高的群体光合速率(CAP),在灌浆后期,不施硫肥处理的小麦CAP分别低于施硫磺粉、石膏、过磷酸钙、硫铵、硫酸钾各处理9.8%、13.3%、11.9%、40.3%和43.7%。施硫提高了小麦旗叶硝酸还原酶（NR）活性,增大了生育后期的物质积累,实现了生物积累量和收获指数的同步提高,最终显著提高了粒重和籽粒产量;施硫促进籽粒灌浆过程中的麦谷蛋白积累量,且对淀粉糊化参数指标如糊化温度、低谷粘度、高峰粘度和最终粘度影响较大,有利于改善小麦品质。在本试验条件下,增施不同种类硫肥对豫麦49产量和品质均有促进效果,其中以过磷酸钙效果较佳。