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1.
Bessa MC Michael GB Canu N Canal CW Cardoso M Rabsch W Rubino S 《Research in veterinary science》2007,83(3):302-310
This study aimed to investigate the relatedness of porcine Salmonella enterica subsp. enterica (S.) serovar Typhimurium strains isolated in Southern Brazil. Sixty-six isolates from pigs belonging to three commercial companies were submitted to phage typing, XbaI-macrorestriction (PFGE), IS200 hybridization, rep-PCR, antimicrobial susceptibility testing, and PCR assay targeting the spvR region. All strains presented a unique rep-PCR pattern and 63 strains had a common IS200 profile. One pulse-type (XA) was the most prevalent (39/66 strains) and included strains of phage types DT177, DT192, DT194 and RDNC. The spvR region was detected in three strains, which harboured plasmids of 90 kb. High rates of tetracycline, sulfonamide and streptomycin resistance were found. Isolates from farms located in different geographic regions but associated to the same commercial companies clustered together and presented a common resistance profile. Results suggested that clonal groups of S. Typhimurium are present in pig commercial companies in Southern Brazil. 相似文献
2.
This work was focused to determine the prevalence and the species diversity of coagulase-negative staphylococci (CoNS) in wild boars, and to study their antimicrobial resistance phenotype and genotype. Nasal samples of 371 wild boars from six Spanish regions were collected for CoNS recovery. The identification was performed by MALDI-TOF mass-spectrometry. Antimicrobial susceptibility for eight antimicrobial agents was studied by disc-diffusion method and the presence of 31 antimicrobial resistance genes by PCR.CoNS were detected in nasal samples of 136/371 animals tested (36.6%), and 161 isolates were obtained (1–3/animal); a high diversity of species was found (n = 17), with predominance of S. sciuri (n = 64), S. xylosus (n = 21) and S. chromogenes (n = 17). Among CoNS isolates, 22.4% showed resistance to at least one antimicrobial tested. Tetracycline-resistance phenotype was the most frequently detected (10.5%), generally mediated by tet(K) gene [associated or not with tet(L)]. Other relevant resistance genes were identified including unusual ones [mecA, erm(B), erm(F), mphC, erm(43), msr(A)/msr(B), lnu(A), dfrG, fexA, and catpC221].This is the first study in which CoNS isolates from wild boars are analysed. The knowledge of antimicrobial phenotype and genotype of CoNS in natural ecosystems is highly important since these staphylococcal species can act as vectors of relevant antimicrobial resistance mechanisms. 相似文献
3.
Molla W Molla B Alemayehu D Muckle A Cole L Wilkie E 《Tropical animal health and production》2006,38(6):455-462
The present study was undertaken to determine the occurrence, distribution and antimicrobial resistance pattern of Salmonella serovars in apparently healthy slaughtered sheep and goats in central Ethiopia. A total 1224 samples consisting of faeces,
mesenteric lymph nodes, liver, spleen, and abdominal and diaphragmatic muscle samples were collected from 104 sheep and 100
goats. Salmonella was isolated from 12 of 104 (11.5%) sheep and 3 of 100 (3%) goats. Of the total 624 and 600 samples examined from sheep and
goats, 18 (2.9%) and 4 (0.7%), respectively, were Salmonella positive. The 22 Salmonella isolates belonged to 9 different serovars. The common serovars isolated were S.
typhimurium, followed by S.
heidelberg, S.
reading, S.
give, and S.
poona. Seven of the 22 isolates (31.8%) were multidrug-resistant to various antimicrobials. 相似文献
4.
植物抗病性及诱导抗性在匍匐翦股颖病害防治中的应用 总被引:1,自引:0,他引:1
针对目前匍匐翦股颖草坪病害防治的弊端,引入诱导植物抗病性的概念,从信号传导、基因表达水平、细胞防御结构和生理响应等层面阐述了诱导抗病性后植株体所发生的变化规律;系统揭示了基于SAR和ISR方式的植物抗病性诱导机理。着意综述了诱导植物抗病性理论和研究成果在匍匐翦股颖病害控制中的应用状况,旨在为匍匐翦股颖新型、环保的草坪病害管理实践提供理论指导。 相似文献
5.
Ashley E. Hill Alice L. Green Bruce A. Wagner David A. Dargatz 《Preventive veterinary medicine》2009,88(4):264-277
Dairy operations in states representing 86% of the United States’ national dairy herd were surveyed regarding the occurrence of common dairy diseases or disorders (digestive, respiratory, gastrointestinal, lameness, mastitis, navel infection) in specified production groups (weaned heifers, unweaned heifers, adult cows), and the most common antimicrobial preparation used to treat these conditions. Within disease and production group, disease frequency and characteristics of primary antimicrobial (drug class, availability, spectrum of activity, extra-label usage, withdrawal times) were compared among herd sizes (30–99, 100–499, and ≥500 cows) using chi-square or t-tests that accounted for the sampling design. The most common diseases were mastitis and lameness in cows, and gastrointestinal disease in unweaned calves, affecting 16, 11, and 15% of the target populations, respectively. Herd-level disease prevalence tended to increase as herd size increased, whereas within-herd prevalence tended to decrease as herd size increased. Broad-spectrum antimicrobials were selected as primary treatments by a majority of operations for nearly all diseases surveyed. When treating gastrointestinal disease, navel infection, or reproductive disorders, a majority of operations selected primary treatments not specifically labeled for that condition. Selection of over-the-counter preparations tended to decrease as herd size increased, whereas selection of broad-spectrum preparations tended to increase with herd size. 相似文献
6.
Noriko IDO Kaori IWABUCHI Yusuke SATO’O Yasuo SATO Masaru SUGAWARA Gakuji YAEGASHI Masaru KONNO Masato AKIBA Kiyoshi TANAKA Katsuhiko OMOE Ikuo UCHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):609-613
Fifty-one Salmonella enterica serovar 4,[5],12:i:-
(S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3
river water strains) which are assumed to be monophasic variants of S.
Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus
variable-number tandem repeat analysis (MLVA) in order to investigate their genetic
diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34
profiles (Simpson’s diversity indices [DI]=0.94, 0.96 and 0.97), respectively. No
correlations were detected between MLVA clustering and PFGE clustering or phage typing.
These results suggested that S. 4,[5],12:i:- originated from multiple
S. Typhimurium ancestors. Two cattle and one pig isolates showing
identical phage types as well as PFGE and MLVA profiles to human isolates
S. 4,[5],12:i:- suggested the existence of the links between human
infections and animal reservoirs. 相似文献
7.
Kaisa S?rén Mats Lindblad Cecilia Jernberg Erik Eriksson Lennart Melin Helene Wahlstr?m Maria Lundh 《Acta veterinaria Scandinavica》2015,57(1)