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《黑龙江畜牧兽医》2014,(11)
为了了解水禽细小病毒分子进化规律,试验采用PCR方法扩增5株水禽细小病毒的结构基因。结果表明:结构基因长度为2 199 bp,编码732个氨基酸。鹅细小病毒VP1蛋白氨基酸序列的同源性为95.1%~100%,番鸭细小病毒之间的同源性为96.9%~99.3%,鹅细小病毒与番鸭细小病毒之间的同源性为85.1%~88.3%;鹅细小病毒VP2蛋白氨基酸序列的同源性为95.2%~100%,番鸭细小病毒之间的同源性为96.6%~99.3%,鹅细小病毒与番鸭细小病毒之间的同源性为85.5%~88.1%;鹅细小病毒VP3蛋白氨基酸序列的同源性为95.0%~100%,番鸭细小病毒之间的同源性为96.8%~99.4%,鹅细小病毒与番鸭细小病毒之间的同源性为88.4%~91.0%。说明鹅细小病毒和番鸭细小病毒均属于不同的进化分支,没有发生重组现象。番鸭细小病毒的2个毒株均存在8个糖基化位点,而鹅细小病毒ZJ毒株为6个糖基化位点,G3与LJY毒株分别缺失582~584 NTT和703~705 NRT。 相似文献
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为研究番鸭细小病毒(MDPV)安徽分离株的遗传变异特征,通过PCR扩增获得了MDPV结构蛋白(VP)基因全长序列AH-MDPV-VP,并将该序列与GenBank中登录的12条MDPV和鹅细小病毒(GPV)VP基因序列进行比对。结果显示,AH-MDPV-VP基因全长2 199bp,包括完整的VP1、VP2和VP3蛋白编码区。MDPV与GPV的VP基因部分序列一致,但具有明显的差异。进化分析显示,MDPV安徽分离株与基因重组型MDPV上海分离株SAAS-SHNH为同一分支,亲缘关系较近。同源性分析显示二者核苷酸序列同源性最高,为99.9%,且AH-MDPV-VP与GPV毒株SHFX1201的序列同源性也有89.5%。此外安徽分离株与其他MDPV的VP1、VP2和VP3基因同源性有逐步下降的趋势,而与GPV则相反呈上升趋势。进一步显示MDPV安徽分离株与基因重组型MDPV上海分离株SAAS-SHNH相似,可能为MDPV和GPV基因重组型水禽细小病毒。 相似文献
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本研究采用PCR技术扩增了鹅和番鸭全长细小病毒基因;其中鹅和番鸭细小病毒非结构蛋白基因(NS)全长为1884bp,编码627个氨基酸;结构基因(VP)全长为2199bp,编码732个氨基酸;序列分析表明:鹅和番鸭细小病毒NS之间的核苷酸和氨基酸同源性分别为80.9%~82.9%和89.5%~91.2%,而相应的鹅细小病毒之间的同源性为93.7%~99.8%和96.8%~99.7%,番鸭细小病毒之间的同源性为98.8%~99.8%和98.6%~99.5%;在核苷酸和氨基酸水平上,鹅和番鸭细小病毒VPI之间的同源性分别为79.7%~88.7%和85.5%~93.3%,而相应的鹅细小病毒之间的同源性为88.8%~99.6%和91.5%~99.2%,番鸭细小病毒之间的同源性为98.1%~99.6%和97.1%~98.8%;番鸭和鹅细小病毒NS和vp1基因的系统进化树分析表明:番鸭和鹅呼细小病毒来自共同的祖先,但随着宿主的不同而演化为不同的分支。 相似文献
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《中国兽医学报》2015,(10):1600-1604
2015年3月以来,我国山东、江苏和安徽等地肉鸭群出现了一种疾病,该病以鸭喙发育不良,舌头外伸为特征。根据症状,暂将该病命名为鸭短喙长舌综合征(duck beak atrophy and dwarfish syndrome,BADS)。从不同地区5个发病肉鸭群采集130余份样品,取5份样品进行病原的分离。细菌培养结果均为阴性(4株大肠杆菌从肝脏常规分离);获得5份鸭胚培养物。该培养物不能凝集鸡红细胞。对5株分离株株进行PCR检测,结果显示鸭瘟病毒、坦布苏病毒、鸭甲肝病毒、鸭星状病毒、鸭呼肠孤病毒、鸭圆环病毒、鹅多瘤病毒、鹅腺病毒、禽网状内皮组织增生征病毒等均为阴性,鹅细小病毒呈阳性。采用基于鹅细小病毒VP3基因的PCR方法对74份样品(肝脏和泄殖腔棉拭子)进行检测,阳性率为100%。该分离株661bp的VP3核苷酸序列与鹅细小病毒和番鸭细小病毒进行分析,结果显示该分离株与鹅细小病毒82-0321v株和SHM319株亲缘关系最近,核苷酸同源性分别为98.8%和98.3%;与番鸭细小病毒同源性较低,在77.6%~78.8%之间。用鸭胚分离的SDLC01株感染1日龄雏鸭能引起鸭上、下喙萎缩、舌头外伸等症状。上述结果表明,从BADS患鸭体内分离得到鸭细小病毒(duck parvovirus,DPV),其可能与BADS具有相关性。 相似文献
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根据国内外已发表的鹅细小病毒(GPV)B株和GD株基因序列,应用DNA Star分子生物学软件设计一对引物,应用PCR技术扩增GPV ZQ株的结构蛋白基因VP2全基因片段。将扩增得到的VP2全基因克隆到pMD18-T载体上,获得的重组质粒经PCR鉴定后进行序列测定。结果表明ZQ株基因大小为1 764 bp,编码587个氨基酸,其基因序列与GPV参考毒株推导的氨基酸序列同源性在89.1%~99.3%之间,差异较大;与番鸭细小病毒(MDPV)参考毒株相比同源性在92.0%~92.5%之间,超过与部分GPV毒株的同源性,推测可能与该毒株来源于番鸭而不是鹅有关,另外也在基因水平上解释了GPV与MDPV在血清学上存在交叉反应的部分原因。 相似文献
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《中国家禽》2017,(4)
试验对鸭源新型鹅细小病毒(Duck derived goose parvovirus,DDPV)DS15株在鸭胚上的增殖特性及对鸭胚的致病力进行了研究。结果显示,鸭胚接种DDPV DS15株以后,96~120 h出现死亡,胚体全身出血,病毒滴度ELD50为10-5.5/0.2 m L。以鸭胚增殖的病毒攻击樱桃谷肉鸭,复制出典型的鸭"大舌病"临床症状,并从发病鸭脏器中分离到病毒。对DDPVDS15株VP2基因进行克隆并并将其因序列与国内外报道的部分鹅细小病毒(GPV)及番鸭细小病毒(MDPV)分离株的VP2基因进行比对和分析。结果表明,DS15株VP2基因与GPV之间的核苷酸和氨基酸同源性分别为89.2%~96.5%和93.4%~97.8%,而与MDPV核苷酸和氨基酸同源性则较低,分别为80.1%~89.3%和87.8%~93.5%;在亲缘关系方面,DDPV DS15株与GPV较近,提示二者可能是由同一病毒进化而来。 相似文献
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番鸭细小病毒国内分离株主要结构蛋白基因的克隆和序列分析 总被引:4,自引:0,他引:4
根据国外已发表的番鸭细小病毒 (MPV) FM株基因组核苷酸序列 ,设计了 1对引物 ,分别对国内 2株番鸭细小病毒分离株 MPV扬州 (YZ)株和 MPV佛山 (FS)株主要结构蛋白 (VP2和 VP3)基因进行 PCR扩增 ,并克隆到p CR3.1T载体 ,经酶切鉴定筛选出阳性克隆质粒并进行核苷酸序列分析比较。结果表明 ,MPV YZ和 MPV FS的VP2 - VP3基因大小均为 176 4bp,编码 5 88个氨基酸。 MPV YZ、MPV FS与 MPV FM核苷酸序列同源性分别为98.5 %和 98.6 % ,氨基酸序列同源性为 97.1%和 97.8% ;MPV YZ与 MPV FS核苷酸序列的同源性为 99.5 % ,氨基酸序列同源性为 98.8%。 相似文献
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为研究鹅细小病毒(GPV)基因遗传变异特征,采集海南某养鹅场疑似鹅细小病毒感染的病料,将其处理后接种番鸭胚成功分离到一株病毒,经PCR鉴定为鹅细小病毒,命名为HN株,并获得了其全基因组序列,将该序列与GenBank数据库中登录的16条鹅和番鸭细小病毒基因序列进行了比对分析。结果显示,该株病毒基因组全长为5 106bp,由ITR、NS、VP构成,其中ITR为444bp,NS1为1 844bp,VP1为2 199bp;HN株与SHFX1201株的NS1基因和VP1同源性最高,分别达到99.8%和99.7%,与番鸭细小病毒株FM的NS1基因同源性最低,为82.7%;与90-0215株VP1同源性最低,为80.1%。HN株的遗传进化树可以看出,GPV可以分成明显的2个基因亚群,HN株与鹅细小病毒匈牙利株(B)、欧洲疫苗株(VG32/1)和台湾株(82-0321V、82-0321、06-0329)均处在第I亚群,且与安徽分离株Y株以及SHFX1201株同源性最接近,番鸭源匈牙利株FM单独处于第Ⅱ亚群。本研究丰富了GPV的数据资料,为研究GPV分类地位以及遗传进化关系提供了依据,同时也为研究GPV流行趋势和疫苗的开发奠定了基础。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献