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Conditions for cell growth of suspension cultures of Cupressus lusitanica, which has high β-thujaplicin productivity, were studied. The medium that provided the highest growth rate was IS-1 medium (pH 5.5), modified
from Gamborg B5 medium containing 32 mM of total nitrogen. Its NO3-N/NH4-N ratio was 30 : 2. The maximum growth represented a 25-fold increase over the initial biomass on a fresh weight basis after
30 days of culture in this medium. The highest cell growth was obtained with an initial pH of 3.5–5.5, but the pH of the medium
settled to about pH 4.0 from any of the initial pH values in this report. The cells cultured under this condition were able
to produce a high level of β-thujaplicin.
Received: September 7, 2001 / Accepted: May 7, 2002
Present address: Teijin Ltd., Ehime 791-8530, Japan
Present address: Q'SAI Co. Ltd., Fukuoka 811-3422, Japan
Part of this report was presented at the 10th international symposium on wood and pulp chemistry, Yokohama, Japan, June 1999
Correspondence to:K. Fujita 相似文献
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抗旱保水剂在墨西哥柏工程造林上的试验研究 总被引:1,自引:0,他引:1
干热河谷石灰岩地区的立地条件差,导致造林成活率低,使用白金子抗旱保水剂结合ABT生根粉、地膜覆盖技术应用于墨西哥柏工程造林,检测结果:(1)显著地提高了造林成活率,达99%,比对照试验地提高了21%-27%;(2)植株生长迅速,长势旺盛,平均高生长达116cm,比对照试验地提高17.5cm,与定植时高度相比,平均增高41cm,地径增粗0.3cm。对比效果明显。该项技术适宜在同类地区的林业工程造林中推广应用。 相似文献
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在实践基础上,总结提出了中山柏全光照自动喷雾双层基质扦插育苗技术,包括全光照弥雾装置、全光照自动喷雾设施及设备、双层基质配置、插条选择和插穗制作、插穗处理、扦插方法、扦插后管理、幼苗越冬管理等方面,与传统扦插方法相比,本方法扦插成活率可提高20%,当年抽梢率提高60%,可给传统扦插繁殖困难树种的育苗提供参考。 相似文献
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Asmare Dejen Demeke 《林业研究》2020,31(1):333-337
In Ethiopia,Cupressus lusitanica and Juniperus procera are important tree species.The incidence of the cypress aphid,Cinara cupressi,which has not been reported before on the exotic Cupressus lusitanica is becoming catastrophic.The appearance of the insect was reported for the first time in 2003 in Ethiopia.However,information is scarce on the status of this species in northeast Amhara state.The objectives of this study were to assess the extent of damage,abundance and status of the cypress aphid on C.lusitanica and J.procera in the protected and cultivated forests of South Wollo,Ethiopia.Results reveal that tree compositions of the three study areas differed.The dominant tree species were C.lucitanica(15–80%),Olea europaea(5–90%)and J.procera(14–70%).The cypress aphid showed significantly higher levels of infestation on C.lusitanica(35–90%)than on J.procera(1–16%).Moreover,there was higher C.lusitanica mortality(40–93%).In contrast,there was low infestation(1–16%)on J.procera and no mortality.The results reveal that the cypress aphid causes enormous losses of C.lusitanica.Therefore,it is recommended that insect surveillance be strengthened and the introduction bioagents be considered and integrated with other insecticides to minimize the degree of C.lusitanica infestation and loss. 相似文献
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《Southern Forests》2013,75(3):113-122
This study compared models for estimating carbon sequestered aboveground in Cupressus lusitanica plantation stands at Wondo Genet College of Forestry and Natural Resources, Ethiopia. Relationships of carbon storage with tree component and stand age were also investigated. Thirty trees of three different ages (5, 12 and 24 years), comprising 10 trees from each stand, were sampled in order to generate dry biomass and carbon data from tree components. Five linear and non-linear biomass and carbon models were compared and evaluated for estimation of overall aboveground carbon, carbon by age groups, and carbon by diameter at breast height (DBH) classes using performance indicator statistics. Among the models compared, a carbon model described by Y = b 0 D 2 H + ? (p-value < 0.001), where D = DBH (in cm), H = total height of the tree (in m), ? = error, and b 0 is a parameter, was found to be the best model for estimation of carbon sequestered aboveground in C. lusitanica plantation stands of the study area. The study also indicated the overall superiority of carbon models over biomass models in estimation of aboveground carbon of C. lusitanica. It was concluded that, for the range of DBH utilised in the current study, the carbon model described can be a useful tool in estimation of carbon storage of C. lusitanica plantations in the study area and other related sites. 相似文献
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施肥对10个墨西哥柏种源幼苗生长的影响 总被引:2,自引:0,他引:2
对温室条件下的10个墨西哥柏种源幼苗进行施肥试验,并测定了其苗高、地径、叶片叶绿素含量和叶片N、P、K浓度。试验设3个处理水平:正常温室养护管理,为对照(CK);施用复合肥N、P、K质量比为1∶1∶1,总养分≥45%(T1);施用复合肥N、P、K质量比为4∶1∶1,总养分≥48%(T2);施用尿素(N 46%)(T3)。结果表明,10个墨西哥柏种源对不同施肥处理的吸收效率各不相同;施肥能显著促进墨西哥柏苗木的生长,不同施肥比例对生长指标的影响各不相同,其中T2>T3>T1;T1、T2处理水平很好的促进了叶绿素的积累,其中T1>T2,而T3对墨西哥柏叶绿素影响不显著;处理T2下的10个种源叶片N、P、K元素浓度与CK差异最显著,其次是T1、T3。 相似文献
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墨西哥柏实生种子园营建及其优树子代测定的研究 总被引:1,自引:0,他引:1
通过种子园与子代测定相结合的方式,在位于滇中的石林林场建立了含102个优树(家系)的墨西哥柏实生种子园,从中探讨了墨西哥柏实生种子园的营建途径。对该种子园的52个墨西哥柏自由授粉优树子代测定的结果表明,墨西哥柏速生,10年生树高、胸径、材积、冠幅的年均生长量分别为1.04 m、1.43 cm、0.009 6m3和0.22 m。生长性状和结实性状家系间的差异达显著水平。树高、胸径、材积、冠幅、结实量的家系遗传力分别为0.76、0.66、0.68、0.74和0.65,单株遗传力分别为0.27、0.16、0.18、0.23和0.16,其生长性状和结实性状呈显著的负相关。从该种子园中评选出了9个优良家系,22个优良单株,其材积遗传增益平均为13.47%和25.2%。此外,还揭示了墨西哥柏10年生期间的生长规律。 相似文献
