Aujeszky's disease virus infection patterns in European wild boar

Evidence of exposure (i.e. seroprevalence) to Aujeszky's disease virus (ADV) is high among wild boars from south-central Spain. This research aims to determine the presence of ADV by molecular detection, and to describe the patterns of ADV infection in wild boars. Tonsils (TN) and trigeminal ganglia (TG) for ADV molecular detection, and sera were collected from wild boars (n = 192) in 39 hunting estates from south-central Spain (2004/2005). A nested polymerase chain reaction (PCR) for a fragment of the ADV surface glycoprotein B was performed on collected tissues. Individual status of presence of viral DNA was tested against explanatory variables by means of a Generalized Linear Mixed Model (GLIMMIX) analysis. Viral detection prevalence was 30.6 ± 6.7%. Although there was an increasing pattern with age and females presented higher prevalences, no statistically significant influence of sex and age was found for viral presence. Molecular testing in TN and TG allowed classifying infection status into (i) ADV negative (in both TN and TG), (ii) only positive in TN, (iii) only positive in TG and (iv) positive in both TN and TG. ADV DNA was statistically more frequently evidenced in TN in females than in males. With the exception of one individual, all wild boars with presence of ADV DNA in TN and TG or only in TG reacted positive in the ELISA. In contrast, animals with only ADV DNA in TN serorreacted positively and negatively. Interestingly, 45% of the PCR positive wild boars (n = 59) were seronegative in the serological test, all of them with viral DNA only in TN. Our results provide evidence for latency of ADV in wild boars and stress the fact that antibody detection based tests may fail to detect a proportion of recently infected animals. This is of great concern since current management schemes in our study promote animal translocation for hunting purposes, with the associated risk of under-detecting ADV infected individuals when using serology to screen for ADV infection.     

Identification of predictors of salmonella shedding in adult horses presented for acute colic

Background

Colic has been associated with shedding of Salmonella. Horses with salmonellosis typically develop diarrhea, fever, and leukopenia. Overlooking additional predictors may result in failure to detect shedding horses and increase environmental contamination.

Objectives

Evaluate associations between signalment and clinicopathologic data during early hospitalization and Salmonella shedding in horses treated for acute colic.

Animals

Horses with acute colic admitted to a referral hospital. A total of 59 horses shedding Salmonella compared to 108 Salmonella‐negative horses.

Methods

Retrospective case‐control study evaluating patient and Salmonella culture data. Associations between variables and Salmonella shedding were identified using logistic regression. Two multivariable models were developed pertaining to (1) information available within 24 hours of admission and (2) clinical findings that developed later during hospitalization.

Results

Variables retained for multivariable model 1 indicated that Warmbloods and Arabians had increased odds for shedding Salmonella, as did horses requiring surgery (OR, 2.52; 95% CI, 1.10–5.75) or having more severe gastrointestinal disease (OR, 2.59; 95% CI, 1.08–6.20). Retained variables for model 2 demonstrated that horses that were treated surgically (OR, 1.60; 95% CI, 0.70–3.62), developed fever >103°F (OR, 2.70; 95% CI, 0.92–7.87), had abnormal leukocyte count (OR, 1.38; 95% CI, 0.61–3.09), or became inappetent and lethargic (OR, 16.69; 95% CI, 4.08–68.24) had increased odds for shedding Salmonella.

Conclusions and Clinical Importance

In horses with acute colic that present without signs of diarrhea, fever, or leukopenia, additional predictors associated with shedding Salmonella could be used to more promptly identify horses likely to shed organisms .     

中国草原火灾风险动态分布特征及管理对策研究

利用我国牧区1991～2005年的草原火灾资料,用统计方法分析了我国牧区草原火灾风险的时空动态分布特征.研究发现,随着经济的发展草原火灾的发生次数呈增长趋势,我国草原火灾风险正不断加大,草原火灾造成的损失也在逐年递增;每年的4月份是我国草原火灾的高峰期,10月份产生一个小高峰期;内蒙古、新疆、甘肃是我国草原火灾经济损失最严重的3个省份.针对我国目前草原防火减灾中出现的问题提出了管理对策.     

Readability Evaluations of Veterinary Client Handouts and Implications for Patient Care

Health literacy and readability are important considerations for veterinary medicine, as veterinarians commonly distribute brochures, leaflets and info-graphics to explain health related issues to pet owners. Educational materials that are poorly comprehended by the intended audience could result in negative, unintended consequences. Thus, the National Institutes of Health (NIH) and the American Medical Association (AMA) have recommended readability levels for text on health information be targeted between the fourth and sixth grade levels to accommodate a highly diverse public. In the context of veterinary medicine, an increased awareness among veterinary professionals and educators regarding readability scores and the tools available to generate “easy-to-read” text would likely benefit client adherence with veterinary recommendations, client satisfaction with their veterinarian, and ultimately, pet health.Therefore, the goal of this study was to evaluate the readability levels of a sample of popular client handout materials prepared expressly for veterinarians to download and distribute to their pet-owning clients. Results indicate that 9 of 10 client brochures were written above the recommended sixth grade reading level. Recommendations for improving readability are provided.     

肉牛犊牛饲养管理及疾病防治

肉牛具有生长周期短、产肉效率高、经济效益高、市场需求大的特征。近年,随消费者的物质生活水平显著提升,对牛肉制品的需求量不断增加,直接促进肉牛养殖产业的发展。肉牛养殖场养殖效益的好坏,受到新生犊牛饲养管理、早期断奶管理、疾病防治等各方面因素影响,做好犊牛养殖管理,能有效减少疫病的发生,避免由于传染性疾病危害造成严重经济损失,保证养殖户的经济效益,为广大消费者提供优质安全的牛肉制品。     

A scoring system for risk assessment of the introduction and spread of bovine viral diarrhoea virus in dairy herds in Northern Italy

To support a voluntary disease control program, this study aimed to develop an integrated scoring system for the risk assessment of bovine viral diarrhoea virus (BVDV) infection in dairy herds in Northern Italy. Sixty-two dairy herds were classified according to their BVDV serological status. Farmers were interviewed using a questionnaire on potential BVDV risk factors. Scores were used to define risk levels for factors related to (1) BVDV introduction (through livestock trade, attendance of animals at shows/exhibitions and grazing common pasture), (2) within-herd spread of BVDV and (3) the results of initial serological testing. The calculated odds ratios were significant for all categories, except for livestock trade. The application of the screening test, the questionnaire and the related risk assessment showed this to be a practical approach to predicting BVDV herd status.     

动物饲养管理与疾病防控的关系

在养殖业生产过程中,动物疾病防控是非常重要的工作,防控工作做得成功与否,事关养殖业成效,直接影响养殖户经济收入,做好动物疾病防控就显得尤为重要了,本人认为要做好动物疾病防控工作,就从平时日常管理做起,建立一整套完善的饲养管理制度,为畜禽提供优异的圈舍环境和优质的饲草料,做好防寒防暑措施,落实动物疫病防控措施,增强动物个体抗病能力,就会有效地降低动物疾病的发生,尤其是传染病发生都可以避免;即使发生传染病,也能及时得到有效控制。在大规模养殖过程中,我们要把重点放在做好饲养管理,确保各项生理指标健康,增加个体抗病能力,从而增强群体抗病能力,而不是忙于治疗个别病畜,否则会因小失大,会使动物疾病防控工作完全陷入被动局面。为此不难看出,动物饲养管理和疾病防控工作有着密切关系,本人通过调查研究、查阅资料,对动物饲养管理与疾病防控的关系进行探讨。     

规模化猪场生物安全体系纵深打造探讨

我国是养猪大国,但并不是养猪强国,生产效率较低,养殖水平虽有提高,但与养猪发达国家之间的差距还比较大,其中生物安全意识淡薄、生物安全体系不完善是一个重要因素。生物安全体系是一种防控猪群疫病的综合措施,完善的生物安全体系可有效切断病原与猪群之间的接触,或者有效降低猪群生存环境或机体内病原数量,使得猪群不发病。文章从猪场生物安全体系建设的主要内容来进行阐述,以期为猪场生物安全体系建设提供思路或借鉴。     

蜜蜂秋季管理要点

科学合理的蜜蜂秋季管理,不仅可以使蜜蜂安全越冬,还能促使蜜蜂来年早春的春繁顺利进行。为了向广大蜂业工作者提供一个全面的蜜蜂秋季管理参考资料,从蜂箱、蜂群、饲喂及病害防治4个方面入手,对蜜蜂的秋季管理要点进行整合。     

春季养鸡疾病防治与养殖管理

自2018年8月受到非洲猪瘟疫情影响,生猪养殖产业受到巨大打击,直接促进家禽养殖产业的发展。随国家对畜禽养殖结构的调整力度不断加大,规模化鸡养殖场的建设数量不断增加。依托规模化鸡养殖产业,带来更多经济收入的同时,也面临较高的疫情防范风险。初春季节外界温度忽高忽低,昼夜温差较大,该时期的鸡身体免疫功能出现下降的现象,造成各种传染性疾病频发。因此做好春季传染性疾病的防治工作,成为需要重点攻克的难题。该文主要结合地区的鸡养殖现状,探讨春季鸡疾病的发生情况,并提出相应的养殖管理措施,更好地控制春季传染性疾病的发生蔓延。     

育成牛的饲养管理

为了降低饲料利用率,提高广大养殖户的养殖效益,本文从育成牛的三个饲养阶段、十个管理方面以及疾病预防,对育成牛的饲养管理做了详细论述,以期为育成牛养殖者提供决策参考.     

A quantitative assessment of the risk of exposure to bovine spongiform encephalopathy via meat-and-bone meal in Japan

The feeding of meat-and-bone meal (MBM) derived from cattle infected with bovine spongiform encephalopathy (BSE) is a major source of BSE infection. The risks of BSE infection via MBM in Japan were examined quantitatively to estimate infectivity to cattle via MBM derived from a single clinically infected animal being rendered. Three routes of exposure were modeled: (i) feeding cattle concentrates containing MBM as an ingredient, (ii) feeding cattle concentrates contaminated with MBM from non-ruminant feed at feed plants and (iii) directly feeding MBM in supplemental form to cattle on farms. The effectiveness of measures designed to restrict the feeding of ruminants with ruminant MBM (feed restriction) as well as differences in the risk of exposure among regions were examined using the model.

The model revealed that the median total infectivity fed to dairy cattle via MBM derived from one infected animal was approximately 0.49 cattle oral ID₅₀ (5th percentile = 0.43 ID₅₀, 95th percentile = 0.54 ID₅₀). This value was reduced by 55% after the addition of MBM to cattle concentrates was restricted in 1996. The risk of exposure in dairy cattle was twice that in beef cattle. Comparisons of regional differences in exposure risk indicated that the risk was highest in a region where 14 of the 20 BSE cases reported to date were born. Our model suggested that the routes of exposure via MBM were unlikely to result in increased propagation of BSE in Japan. Furthermore, despite some regional variation, the risk of exposure declined further after the feed restriction was imposed in 1996.     

Outbreak of Salmonellosis Caused by Salmonella enterica Serovar Newport MDR‐AmpC in a Large Animal Veterinary Teaching Hospital

Background: Nosocomial salmonellosis is an important problem for large animal veterinary teaching hospitals (VTHs). Objective: To describe failure of an Infection Control Program (ICP) that resulted in an outbreak of salmonellosis caused by Salmonella Newport multidrug resistant (MDR)‐AmpC at a large animal VTH. Animals: Sixty‐one animals identified with the outbreak strain of Salmonella. Methods: Retrospective study: Data collected included signalment, presenting complaint, duration of hospitalization, discharge status, and financial information. Phenotypic and genotypic characterization was performed on Salmonella isolates. Results: The outbreak occurred despite an existing ICP; the ICP was reviewed and weaknesses identified. Routine patient surveillance was not performed before or during the outbreak; fecal sampling was triggered only by a patient algorithm based on clinical signs. Sixty‐one animals were infected with the outbreak strain of S. Newport, and the majority were horses (n = 54). Case fatality rate was 36.1%. S. Newport isolates demonstrated high genetic similarity (Dice ≥ 0.96), and all had the MDR‐AmpC phenotype. Environmental persistence of the organism necessitated complete hospital closure, extensive decontamination, and remediation of the facility. A paradigm shift in the relevance of biosecurity in a VTH and the establishment of a stringent ICP were integral components of successful hospital reopening. Conclusions and Clinical Importance: An ineffective ICP resulted in a nosocomial outbreak caused by a MDR S. Newport in a VTH. Closure of a VTH affected all missions of the institution and had substantial financial impact (US$4.12 million).     

新兽药安全评价与评审

对国内外兽药安全评价与风险评估的概念与内容,新兽药研发流程与安全性评价流程,新兽药评审与评价区别进行了论述,并对新兽药安全性评价研究工作目的与结果评价,风险评估与食品安全,特别是每日允许摄入量（ADI）制订和最高残留限量（MRL）的制订等方面进行了论述。对国内企业开发用于食用动物的新兽药有一定指导作用。     

Quantitative assessment of the likelihood of the introduction of classical swine fever virus into the Danish swine population

Classical swine fever virus (CSFV) is a major infectious-disease agent of livestock and causes production losses through increased morbidity and mortality, particularly of young pigs. We identified the pathways for introduction of CSFV into Denmark and assessed the annual probability of introduction (based on a US Department of Agriculture model). We developed pathways based on material from scientific articles, reports from veterinary agencies and custom officers, and consultations with experts in the field. Returning livestock trucks and legal meat imports were the most important pathways for CSFV introduction to Denmark from other EU states with predicted overall likelihood of one or more introductions of CSFV within a median of 130 years (46-280) provided mitigating steps, such as cleaning trucks, were maintained to a very high standard. The likelihood would increase dramatically if these activities were abandoned: one or more introductions within a median of 5.2 years (2-14). The predicted risks from live-animal imports and semen were extremely low given the very few imports of these products. The most important countries for Denmark's CSFV risk are Germany and the Netherlands, though this risk is again predicted to be dramatically reduced as long as mitigating activities are maintained. We predicted the risk from illegal movements of pork into Denmark to be low because little pork enters through this route and only a small fraction of this pork would be fed to pigs.