Effects of carbohydrase enzyme supplementation on performance,eggshell quality,and bone parameters of laying hens fed on maize- and wheat-based diets

1. This study was conducted to determine the effects of enzyme supplementation of maize/wheat-based diets on the performance, egg quality, and serum and bone parameters of laying hens.

2. During the 12-week experimental period, a total of 72 laying hens aged 52 weeks were randomly distributed among 6 experimental groups. Each experimental group contained 4 replicates, each with three birds. The experiment was a randomised design consisting of a 3 × 2 factorial arrangement, with three levels of wheat substitution and two levels of enzyme (xylanase: 1500.00 U/kg, β-glucanase: 100 000 U/kg, cellulase: 1 000 000 U/kg, α-amylase: 160 000 U/kg) inclusion in the diet. Wheat replaced 0, 50, or 100% of maize with or without 1.0 g/kg enzyme supplementation in iso-nitrogenous and iso-caloric experimental diets.

3. Body weight, egg production, egg weight, egg mass, eggshell thickness, and the feed conversion ratio were adversely affected by the wheat-based diet. The eggshell quality parameters decreased with enzyme supplementation to the diet.

4. Wheat-based diets adversely affected calcium and phosphorus concentrations in the tibia, but the addition of the enzymes to the wheat-based diet prevented the negative effects of wheat-based diets on tibia mineralisation in laying hens. The wheat-based diets tended to reduce plasma mineral contents, and the addition of enzymes tended to affect plasma minerals and biomechanical properties of the tibia positively in laying hens.

5. These results indicate that wheat-based diets in aged laying hens adversely affected the mineral metabolism compared with maize-based diets, and the negative effects of wheat on bone mineralisation can be prevented by enzyme supplementation to the diets in laying hens.     

果胶寡糖螯合锌对蛋鸡生产性能、蛋品质和蛋中锌铁含量的影响

试验旨在研究饲粮中添加不同水平果胶寡糖螯合锌(zinc pectic oligosaccharides che-late,Zn-POS)对蛋鸡生产性能、蛋品质和蛋中锌铁含量的影响。选取600只健康的体重和产蛋率相近的22周龄海兰褐蛋鸡,随机分为5组,每组10个重复,每个重复12只鸡。Ⅰ组饲喂基础饲粮,Ⅱ组在基础饲粮中添加268 mg/kg杆菌肽锌,均作为对照组;Ⅲ、Ⅳ和Ⅴ组分别在基础饲粮中添加200、400 mg/kg和600 mg/kg果胶寡糖螯合锌。预饲期1周,正试期8周。结果表明:(1)Ⅱ组和Ⅴ组的产蛋率和平均蛋重均显著高于Ⅰ组(P<0.05),但平均日采食量和料蛋比差异不显著(P>0.05)。Ⅱ组与Ⅴ组相比,产蛋率、平均日采食量、平均蛋重和料蛋比没有显著差异(P>0.05)。各试验组之间平均日采食量和料蛋比差异不显著(P>0.05)。(2)Ⅴ组的蛋黄颜色显著高于Ⅰ、Ⅲ和Ⅳ组(P<0.05)。(3)Ⅱ组蛋中锌含量显著高于Ⅰ组(P<0.05),各试验组蛋中铁含量差异不显著(P>0.05)。由此可见,饲粮中添加果胶寡糖螯合锌可以改善海兰褐蛋鸡生产性能,提高产蛋率,增加平均蛋重和蛋黄颜色。试验中海兰褐蛋鸡饲粮Zn-POS的适宜添加量为600 mg/kg。     

The effects of phytase and xylanase supplementation on performance and egg quality in laying hens

ABSTRACT

1. The aim of this study was to evaluate the effects of phytase and xylanase and their interaction on laying hen performance, egg quality, phosphorus (P) digestibility, phytate breakdown, volatile fatty acid (VFA) production and peptide YY concentration.

2. Two hundred and forty hens were allocated to cages at 22 weeks of age based on a 3 × 2 arrangement with phytase (0, 300 or 1500 FTU/kg) and xylanase (0 or 12 000 BXU/kg) as factors.

3. Phytase increased hen-day production (P < 0.05), daily egg mass (P < 0.05) and P digestibility with increasing levels of phytase (P < 0.001). Phytase fed at 1500 FTU/kg reduced IP6 and IP5 and increased myo-inositol concentration in gizzard digesta (P < 0.05). Phytase fed at 300 FTU/kg reduced IP6 in ileal digesta (P < 0.05); however, IP6 and IP5 were further reduced and myo-inositol increased when phytase was added at 1500 FTU/kg (P < 0.05).

4. Xylanase improved feed efficiency when phytase was fed at 300 FTU/kg (P < 0.05). In the absence of phytase, xylanase reduced dry matter and Ca digestibilities (P < 0.05).

5. Neither phytase nor xylanase had an effect on peptide YY or caecal VFA concentrations.     

低豆粕日粮对产蛋鸡生产性能及蛋品质的影响

试验选用1 600只生产性能相同、体况良好的33周龄罗曼蛋种鸡,随机分为4组,在总蛋白质含量相同和满足必需氨基酸需要量的情况下,用价格较低的蛋白饲料取代蛋鸡日粮中豆粕,研究氨基酸平衡的低豆粕日粮对蛋种鸡生产性能和蛋品质的影响。结果表明:氨基酸平衡的低豆粕日粮对蛋鸡产蛋率、种蛋合格率、健雏率、蛋形指数、蛋比重、蛋壳厚度、哈氏单位等指标无显著影响(P>0.05),但当日粮中的豆粕含量降低8～15个百分点时,蛋种鸡的日平均蛋重、日采食量、受精率、孵化率等显著低于正常豆粕日粮(P<0.05),而考虑综合因素日粮中的豆粕含量降低8个百分点的第1组,与正常豆粕日粮相比,差异不显著(P>0.05),而且能降低饲料成本,可以提高蛋鸡养殖效益,具有实际意义。     

优质蛋白玉米对产蛋鸡生产性能及鸡蛋品质的影响

国外培育的优质蛋白玉米通过大鼠（Mertz，1965；Sproule等，1988），猪（Knabe等，1992；Okai等，1994），肉鸡和蛋雏鸡（Liu等，1993；Osei等，1994）的试验已被证明营养价值优于普通玉米。而国内培育的优质蛋白玉米总能和代谢能与普通玉米相近；氨基酸组成发生了变化，尤其是赖氨酸、色氨酸等含量高于普通玉米所有氨基酸消化率不低于普通玉米。但是其在产蛋鸡上应用的研究还未见报道。为此，进行本次试验，研究优质蛋白玉米（中单9409）在产蛋鸡上应用的效果，为优质蛋白玉米的推广应用提供依据。     

日粮中添加铜对产蛋鸡生产性能及蛋品质的影响

本试验选择五水硫酸铜作为饲料添加的铜源,以产蛋高峰期的海兰褐壳蛋鸡为试验动物,研究日粮中添加60mg/kg的铜对蛋鸡生产性能、蛋品质的影响。试验选取40周龄的海兰褐壳蛋鸡104只,设两个处理,每个处理4个重复,每个重复13只鸡。对照组饲喂基础日粮,试验组饲喂基础日粮 硫酸铜52mg/kg。试验结果表明:1)与对照组相比,试验组能够提高产蛋鸡的各项生产性能,日产蛋重、产蛋率、料蛋比、日采食量均有上升趋势(P>0.05)。2)与对照组相比,试验组的蛋形指数差异不显著(P>0.05),蛋壳厚度变厚(P>0.05),蛋白、蛋黄重以及蛋白蛋黄的比值差异不显著(P>0.05),破软蛋率下降(P>0.05)。     

The performance of laying hens fed on rearing and laying diets containing urea

In an experiment using 2880 light hybrid pullets the effect of urea on laying performance has been assessed. Birds received one of three low‐protein rearing diets from 7–20 weeks of age. The control rearing diet, containing 11.5% crude protein, was compared with two diets containing 13.2% crude protein, one in which the protein was from conventional sources and the other containing 11.5% conventional protein and urea equivalent to 1.7% crude protein. At 20 weeks birds were allocated to one of two laying diets formulated to meet essential amino acid requirements. Half the birds from each rearing treatment received a low‐protein diet (14.7%) and the remaining birds received a 16.4% protein diet which included urea equivalent to 1.7% crude protein. Laying performance was recorded for the next 30 weeks.

Body weights of birds fed on the urea‐containing rearing diet were similar to those of birds fed on the control diet (1.22 kg) and less than those on the 13.2% protein diet (1.29 kg). Urea in the laying diet caused a 2% increase in egg production and tended to cause an improved food conversion efficiency. In contrast, a 3.5% less efficient food conversion was found during laying in birds which had received urea during rearing, compared with birds which had received the low‐protein rearing diet. Birds receiving the 13.2% protein diet, containing no urea, during rearing laid smaller eggs than birds fed on the 11.2% protein rearing diet. A high mortality rate occurred during laying due to lymphoid leucosis but no differences between treatments were found.     

Dietary soluble non-starch polysaccharide level and xylanase supplementation influence performance,egg quality and nutrient utilization in laying hens fed wheat-based diets

The aim of this study was to evaluate the effects of dietary soluble non-starch polysaccharide (sNSP) content and xylanase supplementation on production performance, egg quality parameters, and nutrient digestibility in Hy-line Brown layers from 25 to 32 wk of age. A total of 144 Hy-line Brown laying hens (25 wk of age) were randomly allocated to 1 of 4 wheat-based dietary treatments in a 2 × 2 factorial experimental design, with 36 replicates of individual hens per treatment. The diets were formulated to contain either a high or low sNSP level (at 13.3 or 10.8 g/kg) and were supplemented with either 0 or 12,000 BXU/kg exogenous xylanase. Birds were fed these treatment diets for an 8-wk period, and hen production performance, including daily egg production, average egg weight, daily egg mass, feed conversion ratio and proportion of dirty and abnormal eggs were measured at bird age 25 to 28 wk and 29 to 32 wk. An interaction between sNSP content of the diet and xylanase supplementation was observed on daily egg production from 25 to 28 wk of age (P = 0.018); birds fed the high sNSP diet without xylanase had lower egg production than those fed any other treatment. An interaction between the 2 dietary factors was also observed on hen weight gain at 29 to 32 wk of age (P = 0.014), with birds fed the low sNSP diet with 12,000 BXU/kg xylanase presenting greater weight gain compared to those fed the high sNSP diet with 12,000 BXU/kg xylanase. Feed intake at 29 to 32 wk of age was reduced by xylanase supplementation (P = 0.047). Xylanase supplementation also increased yolk colour score at both 28 and 32 wk of age, and decreased yolk weight at 32 wk of age (P = 0.014, 0.037 and 0.013, respectively). Birds fed the low sNSP diet presented lower protein digestibility (P = 0.024) than those fed the high sNSP diet. Additionally, birds fed high sNSP presented higher shell reflectivity at both 28 and 32 wk of age (P = 0.05 and 0.036, respectively). The influence of duration of feeding the treatment diets on egg quality was also determined. It was observed that egg weight, yolk weight and yolk colour score consistently increased over time, regardless of experimental treatment effects. In contrast, Haugh Unit and albumen height significantly decreased throughout the study period in all treatments, although this was less pronounced in hens fed the treatment with high sNSP and no supplemental xylanase. A reduction in shell breaking strength over time was observed only in hens fed the treatments without xylanase addition, and shell thickness was improved over time only in birds fed the low sNSP diet with xylanase. The impacts of the dietary treatments were largely inconsistent in this study, so a solid conclusion cannot be drawn. However, these findings do indicate that dietary NSP level influences layer production performance, and thus should be considered when formulating laying hen diets. It also proved that further research is warranted into how to optimize the benefits of xylanase application in laying hens.     

加丽素红对蛋鸡生产性能、蛋品质及血清生化指标的影响

选用160只360日龄的京红1号蛋鸡,随机均分为4个处理组,每个处理4个重复,每个重复10只鸡,探讨日粮中不同加丽素红添加量(15,30,60 mg/kg)对蛋鸡生产性能、蛋品质及血清生化指标的影响。结果表明,日粮中添加不同剂量的加丽素红会显著降低蛋鸡的饲料转化率(P0.05),而对蛋鸡的其他生产性能、血清生化指标均无显著性影响(P0.05)。对于蛋品质,试验组蛋黄色度与对照组比较有极显著性差异(P0.01),对其他指标均无显著性影响;说明加丽素红能明显增加蛋黄色度,可以作为外源性色素添加剂,但是不会对蛋鸡的健康产生危害。     

不同锌源及水平对蛋鸡生产性能、蛋品质、血液生化指标以及蛋锌含量的影响

试验研究不同锌源及水平对蛋鸡生产性能、蛋品质、血液生化指标以及蛋锌含量的影响。选用21周龄健康的罗曼蛋鸡720只,随机分为5个处理,每个处理6个重复,每个重复24只鸡。对照组饲喂基础饲粮(锌含量测定值20.56 mg/kg),其他4组分别在基础饲粮中添加35、70 mg/kg的氨基酸络合锌(Av-Zn)或硫酸锌(锌含量实测值分别为40.11、80.39、36.62、76.10 mg/kg)。试验预试期7 d,正试期56 d。结果表明:(1)锌源之间对平均日蛋重和损蛋率差异显著(P<0.05);(2)锌源及水平对蛋壳重有显著影响(P<0.05);(3)不同锌源对蛋鸡血清锌离子、SOD活性以及Cu Zn-SOD活性均没有显著影响(P>0.05);不同水平的锌对蛋鸡血清锌离子有极显著影响(P<0.01)、对Cu Zn-SOD活性有显著影响(P<0.05),对SOD活性并没有显著影响(P>0.05);(4)锌源对第14、28、42、56 d蛋黄锌的影响差异显著(P<0.05);锌水平对第14、28、42 d蛋黄锌含量的影响差异不显著(P>0.05),但对第56 d蛋黄锌含量差异极显著(P<0.01)。锌源和锌水平之间对14、28、42、56 d蛋黄锌含量的影响差异不显著(P>0.05)。由此可见,添加70 mg/kg氨基酸络合锌能生产富锌鸡蛋,同时对蛋鸡的正常生产没有影响。     

日粮中添加女贞子粉对蛋鸡生产性能和蛋品质的影响

选择150只49周龄、平均体重(1.99±0.17)kg的健康海兰褐蛋鸡,采用单因素完全随机分组试验设计,随机分为对照组(基础日粮)、试验组Ⅰ(基础日粮+0.5%女贞子粉)、试验组Ⅱ(基础日粮+1%女贞子粉)。每组设5个重复,每个重复10只蛋鸡,试验期为8周,研究了女贞子粉对蛋鸡生产性能和蛋品质的影响。结果表明:与对照组相比,试验组Ⅰ、Ⅱ产蛋率分别提高了4.86%和11.75%(P0.05)。试验组Ⅰ蛋黄颜色显著高于对照组9.60%(P0.05)。日粮中添加女贞子粉可以提高蛋鸡产蛋率,改善蛋黄颜色,进而提高蛋鸡的生产性能和蛋品质。     

有机源锌和锰对蛋鸡生产性能、蛋壳和骨质量的影响

文章旨在研究日粮添加有机源锌和锰替代无机锌和无机锰对蛋鸡生产性能、蛋壳和骨骼质量的影响。试验选择31周龄产蛋性能接近的罗曼粉壳蛋鸡616只,随机分为7组,每组4个重复,每个重复22只。基础日粮锌和锰含量分别为50和30 mg/kg,对照组饲喂基础日粮,分别添加氧化锌和硫酸锰(锌40 mg/kg,锰60 mg/kg)。处理组分别在基础日粮中添加20 mg/kg有机锌,30 mg/kg有机锰、20 mg/kg有机锌+30 mg/kg有机锰、40 mg/kg有机锌、60 mg/kg有机锰、40 mg/kg有机锌+60 mg/kg有机锰,上述含量均以相应元素计。试验共进行7周。结果显示:日粮用有机锌或锰替代50%或100%无机锌、锰对蛋鸡生产性能相关参数均无显著影响(P> 0.05)。对照组相比,有机锌或锰替代无机锌或锰的比例对32、46、60和74周龄蛋鸡蛋壳相对重量、蛋壳密度和蛋壳厚度均无显著影响(P> 0.05)。与对照组相比,30 mg/kg有机锰、20 mg/kg有机锌+30 mg/kg有机锰组的蛋壳强度显著提高(P <0.05)。40 mg/kg有机锌+60 mg/kg有机锰组较无机锌、锰组显著提高了60、74周龄及全期蛋壳强度(P <0.05)。日粮添加有机锌或锰对胫骨物理参数及胫骨和趾骨灰分含量均无显著影响。结论 :日粮添加有机锌、锰替代无机锌锰对蛋鸡产蛋性能和骨质量无影响,但可以降低蛋鸡由于年龄升高对蛋壳强度的负面影响。     

酵母菌培养物对蛋鸡生产性能及蛋品质的影响

270只19周龄的ISABrow母鸡随机分3组处理,每个处理5个重复,研究酵母培养物对产蛋鸡产蛋性能和蛋品质的影响。试验为期16周。酵母培养物在试验中的添加水平分别为0%、0.1%、0.2%。试验中采用的玉米-豆粕型基础日粮中粗蛋白质为16.0%,能量为2800kcal/kg。试验结果表明,饲喂添加0.1%酵母培养物组产蛋鸡的生产性能和每日产蛋量显著提高(P<0.05);各处理组之间的蛋重、饲料摄入和饲料转化率无显著差别;试验组与对照组的蛋壳质量、哈氏单位、蛋黄颜色和蛋黄中脂肪酸含量也无显著差别。     

Productive and reproductive performance and egg quality of laying hens fed diets containing different levels of date pits with enzyme supplementations

Two experiments were conducted to investigate the effect of phytase and/or multienzymes (protease, amyloglucoidase, xylanase, B-glucanase, cellulose, and hemicellulase) on improving the utilization of date pit (DP) in laying hens. In the first one, DP completely replaced corn in four isocaloric and isonitrogenous diets: (1) DP50 without additives, (2) DP50?+?500 FTU phytase/kg, (3) DP50?+?0.1 % multienzymes, and (4) DP50?+?500 FTU phytase/kg?+?0.1 % multienzymes, in addition to the diet without DP. In the second experiment, DP was included at 0 %, 15 %, and 30 % in isocaloric and isonitrogenous diets. Meanwhile, diets containing 15 % and 30 % DP (DP15 and DP30) were fed without or with 500 FTU phytase/kg diet and/or 0.1 % multienzymes. In both experiments, each diet was fed to six groups of five hens housed individually (520 cm² per hen) during 30–42 and 28–42 weeks in the first and second experiment, respectively. In the first experiment, productive performance and shell quality of laying hens significantly decreased due to complete substitution of corn, but fertility and hatchability were not affected. Phytase, multienzymes supplementation did not restore laying performance to the control level. The results of experiment 2 indicated that DP could be included in laying hens diets up to 30 % when supplemented with multienzymes.     

甪里艾叶粉对蛋鸡产蛋性能与蛋品质的影响

为了研究甪里艾叶粉对蛋鸡产蛋性能与蛋品质的影响,试验选用50周龄健康状况良好、生产性能相近的海兰褐蛋鸡288只,随机分为四组,一个对照组,三个试验组,各组甪里艾叶粉分别添加0、1%、2%、3%,每组6个重复,每个重复12只鸡,预试期为7 d,试验时间为42 d。结果显示:(1)甪里艾叶粉对产蛋性能的影响:试验各组的平均日产蛋重均高于对照组(P0.05),分别提高了2.62%、3.58%、2.41%;随着艾叶粉添加量的增加,平均日采食量显著减少(P0.05),分别减少了2.76%、3.29%、3.62%;试验各组料重比均显著低于对照组(P0.05),分别降低了5.10%、6.63%、5.61%;随着甪里艾叶粉添加量的增加,产蛋率分别提高了2.85%(P0.05)、3.99%(P0.01)、5.93%(P0.01)。试验各组的蛋重与对照组均无显著差异(P0.05)。(2)甪里艾叶粉对蛋品质的影响:甪里艾叶粉可显著提高蛋的比重(P0.05),其中添加量为2%时差异极显著(P0.01)。各组间的蛋形指数、哈氏单位、蛋黄颜色差异均不显著(P0.05)。但试验各组的蛋白高度分别提高了8.92%(P0.05)、11.29%(P0.05)、10.21%(P0.05),哈氏单位则相应提高了4.51%、5.67%、5.69%。各组间的蛋壳指标差异不显著(P0.05)。试验各组的蛋白比率、蛋黄比率差异不显著(P0.05),随着甪里艾叶粉添加量的增加,蛋壳比率逐渐降低,其中添加2%和3%的甪里艾叶粉可显著降低蛋壳比率(P0.05)。甪里艾叶粉可以改善海兰褐蛋鸡的产蛋性能,蛋的比重和蛋白高度显著提高,蛋壳比率呈降低的趋势。     

能量水平对粉壳蛋鸡生产性能及蛋品质量的影响

为研究不同能量水平对产蛋性能和蛋品质量的影响,试验选用504只194日龄罗曼粉蛋鸡,试验共设3个处理,每个处理5个重复,每个重复33～35只,分别饲喂三个能量水平(10.88、11.09、11.30 MJ/kg)的日粮。试验共进行30 d。试验结果表明,各处理组在采食量、产蛋率、平均蛋重、料蛋比、蛋壳强度和哈夫单位等方面差异不显著;表明能量水平在50～100 kcal/kg范围内波动,对蛋鸡生产性能和蛋品质量未产生显著影响。     

Effects of an aqueous alfalfa extract on production performance, egg quality and lipid metabolism of laying hens

The present study was conducted to evaluate the effect of an aqueous alfalfa extract (AAE) on production performance, egg quality and lipid metabolism of laying hens between 28 and 36 weeks of age. Four groups of commercial hens (Hy-Line Brown) were fed with diets containing 0, 0.05%, 0.1% and 0.15% AAE. Dietary AAE had no effect on egg production parameters (p > 0.05). Shell strength was increased (linear and quadratic effects; p < 0.05) with gradient addition of AAE. Furthermore, shell strength was notably higher (p < 0.05) in 0.1% and 0.15% AAE groups than the control group. With an exception of serum low and high density lipoprotein cholesterol, serum total triglycerides and liver cholesterol concentration (day 42), lipid indices in yolk, serum and liver were lowered (linear and quadratic effects; p < 0.05) at day 42 and day 56 as AAE in diets increased, as well as the enzyme activities of acetyl-CoA carboxylase (ACC) and β-hydroxy-β-methylglutaryl-CoA (HMG-CoA) reductase. In relation to the control group, cholesterol and triglycerides in yolk, liver and serum and serum very low density lipoprotein cholesterol were lower in 0.15% AAE-treated birds at day 42 and day 56. It also significantly decreased activities of ACC, HMG-CoA reductase and fatty acid synthetase (FAS) at day 56. The 0.1% AAE decreased triglycerides in yolk, liver and serum, as well as the activities of ACC and FAS at day 56. These data suggest that dietary AAE could be applied to improve egg shell quality and modify the lipid status of laying hens and eggs.     

低营养水平日粮添加复合酶对蛋鸡生产性能、蛋品质和抗氧化的影响

本研究旨在探讨不同营养水平日粮添加复合酶对蛋鸡生产性能、蛋品质、抗氧化及盲肠微生物含量的影响.试验选择产蛋率接近的42周龄蛋鸡504只,随机分为3组,每组4个重复,每个重复42只.对照组饲喂正常蛋白能量日粮,T1组饲喂低蛋白能量日粮,T2组饲喂低蛋白能量日粮+100?IU/kg复合酶.在为期9周的饲养试验后测定相关指标...