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1.
1. The objective of this study was to determine the prevalence and antimicrobial resistance of Arcobacter spp. isolated from different species of retail poultry meat in Iran.

2. From August 2012 to April 2013, a total of 540 raw poultry meat samples from chicken (n = 100), turkey (n = 100), quail (n = 100), partridge (n = 80), duck (n = 50), ostrich (n = 60) and geese (n = 50) were purchased from randomly selected retail outlets in Shahrekord, Isfahan, Sari and Rasht, Iran.

3. Using culture techniques, 71 of 540 poultry meat samples (13.1%) were positive for Arcobacter spp. The highest prevalence of Arcobacter spp. was found in chicken meat (28.0%), followed by quail (12.0%), duck (11.4%), turkey (11.0%), geese (8.0%), partridge (7.5%) and ostrich (3.3%) meat. The number of A. butzleri isolated from poultry meat samples (90.1%) was significantly higher than A. cryaerophilus (7.1%) and A. skirrowii (2.8%). Significantly more poultry meat samples were found to contain Arcobacter spp. by the PCR assay than by the culture method.

4. Susceptibilities of Arcobacter isolates were determined for 14 antimicrobial drugs using the disk diffusion method. All of the 71 Arcobacter isolates tested were resistant to one or more antimicrobial agents. Resistance to cephalothin and vancomycin (95.8%) was the most common finding, followed by resistance to methicillin, azithromycin and ampicillin. All Arcobacter isolates were susceptible to gentamicin, streptomycin, tetracyclin and kanamycin.

5. The results of this study indicated the importance of poultry meat, especially chicken meat, as potential sources of Arcobacter spp. infection in people. Furthermore, the strains indicated resistance to a broad spectrum of antibiotics.  相似文献   


2.
1. The objective of this study was to evaluate the effects of dry-ice decontamination on equipment and carcase surfaces in a poultry slaughterhouse and to present an effective alternative method to the conventional decontamination processes.

2. Appreciable reductions occurred in total aerobic mesophilic bacterial counts of surface swab samples treated with dry ice (maximum difference 3.92 log cfu/100 cm2).

3. After dry-ice treatment, Listeria spp. were detected on surfaces of pluckers and chiller cylinders, whereas Salmonella spp. were totally inhibited.

4. A dry-ice spraying application was more effective than a dry-ice immersing application on total aerobic mesophilic bacteria and yeast and mould counts on poultry carcases.

5. Dry-ice treatment has advantages over conventional processes. Unlike other decontamination techniques, there are no residues, so no need to wash off chemical residues from surfaces as it removes contaminants effortlessly and is environmentally friendly.

6. Dry-ice blasting of production equipment can reduce the microbial load and has potential for use in the poultry industry.  相似文献   


3.
1. The main aim of this work is to develop a robust method to generate a microbial mixture which can successfully degrade poultry feathers to overcome environmental problems.

2. Four different alkaliphilic microbes were isolated and shown to degrade poultry feathers.

3. Two of the isolates were phylogenetically identified as Lysinibacillus and the others were identified as Nocardiopsis and Micrococcus.

4. The best microbial co-culture for white and black feather degradation was optimised for pH, temperature and relative population of the isolates to achieve almost 96% of degradation compared with a maximum of 31% when applying each isolate individually.

5. The maximum activity of keratinase was estimated to be 1.5 U/ml after 3 d for white feathers and 0.6 U/ml after 4 d for black feathers in a basal medium containing feather as the main carbon source. Additionally, non-denaturing polyacrylamide gel electrophoresis showed 4 and 3 protease activity bands for white and black feather, respectively.

6. This study provides a robust method to develop potential new mixtures of microorganisms that are able to degrade both white and black feathers by applying a Central Composite Design.  相似文献   


4.
5.
1. Furazolidone, a nitrofuran antibiotic, was prohibited from the use in food-producing animals in the European Union (EU) in 1997. In 2002, the EU restricted the import of poultry meat and aquaculture species from countries where furazolidone residues had been detected.

2. By 2004, however, residues of the side-chain metabolite, 3-amino-2-oxazolidinone (AOZ) of furazolidone, were detected in chicken meat produced in Northern Ireland.

3. With the random spread of positive results across farms of a single integrated organisation, including organically reared flocks, it seemed unlikely that the source of residues was due to illegal use of the drug, but more likely caused by a source of contamination.

4. Potential sources investigated were as follows: furazolidone contamination of feedstuffs, a “hot spot” of furazolidone in poultry houses, contamination occurring within breeding stocks and transferred with the birds to broiler growing houses, and furazolidone contamination of the water supply.

5. Furazolidone contamination was associated with birds reared in houses more than 10 years old.

6. Contamination was traced to the water supply of poultry houses, where un-dissolved furazolidone, legally administered prior to 1997, had settled to the bottom of water storage tanks. It remained un-disturbed until 2004 when the integrator changed the procedure for cleaning water tanks between crops of birds.

7. The use of Proxitane, a hydrogen peroxide disinfectant, caused effervescence within the tank such that small quantities of furazolidone were dissolved, delivered to birds via drinkers and subsequently caused residues in the broiler meat.

8. The environmental impact of the contamination was investigated by testing soil and grass from land adjacent to an organic poultry house to which birds had access.

9. Mechanisms of contamination and how residues may be spread throughout a large integrated poultry system are not restricted to furazolidone. Incidents of contamination are even more likely when using licensed drugs where the drugs may be present on-farm in large quantities.  相似文献   


6.
7.
1. The purpose of this work was to support decision-making in poultry farms by performing automatic early detection of anomalies in egg production.

2. Unprocessed data were collected from a commercial egg farm on a daily basis over 7 years. Records from a total of 24 flocks, each with approximately 20 000 laying hens, were studied.

3. Other similar works have required a prior feature extraction by a poultry expert, and this method is dependent on time and expert knowledge.

4. The present approach reduces the dependency on time and expert knowledge because of the automatic selection of relevant features and the use of artificial neural networks capable of cost-sensitive learning.

5. The optimum configuration of features and parameters in the proposed model was evaluated on unseen test data obtained by a repeated cross-validation technique.

6. The accuracy, sensitivity, specificity and positive predictive value are presented and discussed at 5 forecasting intervals. The accuracy of the proposed model was 0.9896 for the day before a problem occurs.  相似文献   


8.
1. This paper reviews the use of botanical extracts in the control of coccidial infection in poultry.

2. Some plants and their respective volatile oils and extracts have the potential to alleviate coccidiosis and reduce its severity.

3. Most plant bioactives improve some, but not all, aspects of coccidiosis with variable effectiveness against different species of Eimeria.

4. Difficulties in comparing research findings have arisen from the use of different experimental models, different active components and infectious dose of Eimeria.

5. Current knowledge of their potential anti-coccidial effects may provide guidance for the use of botanical extracts in the control of the coccidiosis.  相似文献   


9.
1. The carcasses of 66‐ and 73‐d‐old stag turkeys implanted with 20 mg trienbolone acetate (TA) at 49 d of age were analysed and the results compared with those of untreated controls.

2. Treatment with TA improved growth, increased carcass moisture and tended to decrease carcass fat and ash; protein content was not altered by implantation.

3. Treatment with TA produced a marked improvement in efficiency of conversion of dietary protein to carcass protein.

4. Carcass formation of 70‐ and 77‐d‐old turkeys of both sexes implanted with 20 mg TA at 49 d of age was not markedly different from that of controls in terms of the relative proportions of the major body components nor in relative skeletal proportions.

5. Implantation with TA reduced linear and appositional skeletal growth both relative to soft tissue growth and relative to skeletal growth of control turkeys.

6. The rate of bone remodelling was reduced more than the rate of appositional growth so that bone cortical thickness was increased.

7. The changes in the skeleton effected by TA are consistent with the hypothesis that the drug acts by decreasing the rate of protein turnover, with catabolism being depressed more than anabolism.  相似文献   


10.
1. The present study investigated the effects of encapsulated benzoic acid (BA) supplementation in broiler feed on performance and gastrointestinal microbiota.

2. Eighty broilers were randomly divided into two groups. Birds in the control group were fed on maize–soybean-based diets. Birds in the treatment group were provided the same diet supplemented with 2 g/kg BA encapsulated in a vegetable oil matrix.

3. At the end of the trial (d 35), pH, bacterial composition and metabolites were determined in the crop, jejunum, ileum and caecum.

4. Growth performance variables and pH were not significantly different.

5. BA concentration decreased rapidly in the proximal gut. However, the treatment diet showed higher BA in the crop, jejunum, ileum and caecum.

6. Total lactate in the crop and D-lactate in the jejunum was higher in the BA treated group. Caecal total and branched chain fatty acids were decreased due to the treatment.

7. Lactobacilli populations were significantly altered by BA supplementation. A trend for increased lactobacilli was observed in the crop, while it became significant in the jejunum and ileum. Lactobacillus species responded differently to the treatment. Four of 5 measured Lactobacillus species, particularly in the ileum, followed the course observed for total lactobacilli; only Lactobacillus salivarius was not modified.

8. Correlation analysis showed that BA modified the intestinal microbiota. Lactobacilli correlated negatively to all studied clostridial clusters and enterobacteria. Clostridial clusters IV and XIVa were significantly increased in the jejunum, whereas only clostridial cluster XIVa was increased in the caecum.

9. Encapsulated BA modified the intestinal microbiota which can lead to the conclusion, that the main beneficial mode of action of BA in the gut appears to be the enhancement of lactic acid bacteria, which in turn may act as a vanguard against pathogens.  相似文献   


11.
1. The objective of the experiment was to characterise the genotypic and phenotypic differences between presumptive commensal E. coli and avian pathogenic E. coli (APEC) of poultry.

2. DNA was extracted from 65 confirmed APEC E. coli from chicken, 100 presumptive commensal E. coli from healthy turkey and 35 from healthy chicken. Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and virulence factors genotyping was performed to characterise genetic features.

3. Carbon source utilisation and antimicrobial susceptibility tests were performed to characterise phenotypic features of isolates.

4. The genetic divergence between E. coli strains tested by ERIC-PCR profiles and virulence-associated genes showed a clear genetic separation between E. coli APEC and turkey E. coli strains.

5. The carbon utilisation profile of turkey isolates was different from chicken and APEC strains; whereas antimicrobial susceptibility was highest for turkey isolates (53%), and lowest for APEC strains (33.8%).

6. The study showed a significant negative correlation between utilisation of arabitol and adonitol with different virulence determinants tested, which suggests that the ability to utilise some uncommon carbon sources may be used to discriminate between presumptive commensal E. coli and APEC.  相似文献   


12.
1. The ovulatory cycle of the domestic hen (Gallus domesticus) was postulated to be the result of the interaction of two independent systems.

2. A circadian system was postulated to control the restriction of ovulation to an 8‐h period of the day under conventional 14 h light: 10 h dark regimes.

3. The final phase of follicular maturation was postulated to commence after ovulation of the preceding ovum in the hierarchy.

4. Ovulation was postulated to occur when a mature follicle was present in the ovary during the appropriate phase of the circadian‐linked system.

5. The predicted times of oviposition were within the standard error of the observed times of oviposition under 21‐, 24‐ and 28‐h photoschedules.

6. It was concluded that this hypothesis for the control of the ovulatory cycle of the hen is consistent with current knowledge.  相似文献   


13.
1. The effect of reducing food intake to 75% of the ad libitum intake was determined from hatching to 8 weeks in young Light Sussex chickens.

2. Restricted birds were lighter throughout the experiment.

3. Relative adrenal weight tended to be greater in restricted birds but the difference decreased with time.

4. There was no depletion of adrenal cholesterol: from week 5 there was a significantly greater amount in the adrenals of restricted birds.

5. After 1 week of restriction plasma corticosterone concentration was 73% greater than in controls. It decreased progressively, falling within the normal range at 5 weeks.

6. Restricted birds were hypoglycaemic from weeks 2 to 7 and hyper‐lipacidaemic throughout. A negative correlation between plasma glucose and free fatty acids was found.  相似文献   


14.
1. The effects of age and sex on the proximate composition and inorganic constituents in edible offal and whole blood from a commercial British broiler chicken strain were studied.

2. Although the protein content of all tissues varied with age, the only statistically significant effect was on the level of protein in the gizzard.

3. The moisture content of the gizzard was significantly affected by age and blood by age and sex.

4. The fat content of the gizzard was significantly affected by age, as was the effect of sex on the fat content of blood.

5. The ash contents of heart and gizzard were significantly affected by age.

6. The phosphorus contents of the gizzard, heart and liver were significantly affected by age, as were the chloride content of heart and the calcium content of the liver. Blood potassium was significantly affected by sex. Fluctuations in the contents of other elements are discussed.

7. The variability in organ composition is discussed with respect to the degree of trimming and preparation of the samples.  相似文献   


15.
1. Genetic adaptation was investigated in broilers selected for seven generations on a normal (A) or a low (B) protein diet.

2. Protein and energy metabolism were studied in males from these selected lines fed on a diet of intermediate protein content.

3. All selected birds retained more nitrogen than those studied 10 years previously.

4. There was no difference in nitrogen retention between groups, although relative growth rate of group B birds was higher.

5. Heat productions relative to gross energy intake were 0.38 (group B) and 045 (group A). Energy retentions relative to gross energy intake were 0.39 (group B) and 0.35 (group A); the difference being primarily due to higher fat retention in group B.

6. Using a common maintenance requirement for metabolisable energy, group B utilised metabolisable energy for growth (0.78) better than did group A (0.71).

7. At 53 d of age plasma glucose (10%) and insulin (50%) were higher in group B than in group A.  相似文献   


16.
1. The expression of insulin-like growth factor-1 (IGF1) and myostatin (MSTN) mRNA in breast and leg muscle was quantified in 70-d-old Taihu and Wanxi geese by using a Multiplex Competitive Fluorescent–PCR method and the correlations between mRNA levels and carcass traits were analysed.

2. IGF1 mRNA expression in breast muscle in Taihu geese was significantly higher than that in Wanxi geese and the MSTN mRNA level in leg muscle in Taihu geese was significantly higher than that in Wanxi geese.

3. There was no significant difference in breast muscle MSTN or leg muscle IGF1 mRNA expression between the two breeds.

4. Within the same breed, the IGF1 mRNA expression in leg muscle of male geese was significantly higher than that in female geese, and MSTN mRNA expression in leg muscle was significantly higher than that in breast muscle.

5. There was no difference in the IGF1 mRNA expression between tissues.

6. There was a positive correlation between IGF1 mRNA and MSTN mRNA and a negative correlation between IGF1 mRNA expression of breast muscle and leg muscle ratio.

7. In Wanxi geese, MSTN mRNA expression in leg muscle was negatively associated with body weight and leg muscle weight.  相似文献   


17.
1. The present study investigated the effects of dietary supplementation with Gynura procumbens on egg yolk and serum cholesterol and triglycerides, excreta microflora, laying performance and egg quality.

2. A total of 160 Hy-Line Brown layers (45 weeks old) were randomly assigned into 4 treatments on the basis of laying performance. Each treatment had 4 replicates with 10 birds each.

3. Dietary treatments were basal diet supplemented with 0 (control), 2.5, 5.0 and 7.5 g/kg diet G. procumbens during 56-d feeding period.

4. Serum (d 21, 42 and 56) and egg yolk (d 28, 42 and 56) cholesterol and triglycerides concentrations were linearly reduced with increasing dietary concentrations of G. procumbens.

5. Increasing dietary concentrations of G. procumbens linearly reduced the excreta total anaerobic bacteria (d 28), Clostridium sp. and Escherichia coli (d 28 and 56) populations.

6. Overall egg production and egg mass were linearly increased, and overall feed efficiency was linearly improved with increase in dietary G. procumbens.

7. Dietary increasing concentrations of G. procumbens linearly improved egg yolk colour (d 28 and 56) and breaking strength of eggs (d 56).

8. The results obtained in the present experiment indicate that dietary supplementation with G. procumbens could reduce the egg yolk cholesterol, suppresses harmful excreta microflora and improves layers performance.  相似文献   


18.
1. A comparison of the productive performances of the Sinai Bedouin fowl (from the Sinai desert), the commercial White Leghorn and their reciprocal crossbreds was made under uniform normal conditions.

2. The Sinai produced significantly fewer, smaller eggs, resulting in lower egg mass output (g/bird d), than the Leghorn and the crossbreds.

3. Egg weight of both crossbreds was intermediate between Sinai and Leghorn but laying rate was closer to that of the Leghorn.

4. Differences in egg mass output apparent at 8 to 10 months of age decreased considerably with age.

5. These findings suggest that selection or crossbreeding of the Sinai might improve productive performance while maintaining improved eggshell quality.  相似文献   


19.
1. Infectious diseases have a large impact on poultry health and economics. Elucidating the pathogenesis of a certain disease is crucial to implement control strategies.

2. Multiplication of a pathogen and its characterisation in vitro are basic requirements to perform experimental studies. However, passaging of the pathogen in vitro can influence the pathogenicity, a process targeted for live vaccine development, but limits the reproduction of clinical signs.

3. Numerous factors can influence the outcome of experimental infections with some importance on the pathogen, application route and host as exemplarily outlined for Histomonas meleagridis, Gallibacterium anatis and fowl aviadenoviruses (FAdVs).

4. In future, more comprehensive and detailed settings are needed to obtain as much information as possible from animal experiments. Processing of samples with modern diagnostic tools provides the option to closely monitor the host–pathogen interaction.  相似文献   


20.
1. A 31P topical magnetic resonance study of whole chicken's eggs in vivo is described.

2. Resonances from adenosine triphosphate, phosphocreatine, inorganic phosphate and phosphorous storage protein were observed. These changed in relative intensity as the embryo developed.

3. No ill effects due to exposure to radiofrequency radiation or magnetic fields were observed.

4. It is concluded that topical magnetic resonance can make a useful contribution to the study of embryonic development in avian eggs.  相似文献   


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