猪副嗜血杆菌病的诊治及防控对策

云南省会泽县上村乡大河村某猪场发生了疑似猪副嗜血杆菌引起的疾病.临床症状表现为体温升高,精神沉郁,食欲减退,消瘦,咳嗽,腹泻,关节肿胀及神经症状..剖检见肺脏呈纤维素性胸膜肺炎,胸腔内有大量淡红色液体、纤维性渗出物及凝块;腹腔内有多量透明黄褐色漏出液,有的呈胶冻状凝块;心包膜上有奶酪样坏死,并与心脏粘连.肺臌胀,表面覆有大量纤维素性渗出物,并与胸壁粘连,镜检肺泡充满水肿液或纤维蛋白和红细胞.全身淋巴结肿大,呈浆液性卡他性炎,暗红色;取心血、肝、脾涂片,革兰氏染色,镜检,见有革兰氏阴性球杆菌.将病菌接种于血液琼脂平皿上,置37℃温箱培养48 h,革兰氏染色,镜检该菌同涂片细菌.动物回归试验,病理变化同自然感染病例.用纸片法对32种药物进行药敏试验,结果该菌对阿米卡星、美西林、磺胺甲基异恶唑3种药物高度敏感.结合本病的流行情况、临床症状、病理变化和实验室检查,确诊为猪副嗜血杆菌感染.经采取综合防控措施,有效控制了该疫病的扩散.     

猪副嗜血杆菌病的诊治体会

<正>2014年11月16日,云南省长宣威市双龙街道左所社区某猪场发生了疑似猪副嗜血杆菌引起的疾病,临床出现体温升高、精神沉郁、食欲减退、消瘦、咳嗽、腹泻、关节肿胀及神经症状。剖检见肺脏呈纤维素性胸膜肺炎,胸腔内有大量淡红色液体、纤维性渗出物及凝块;腹腔内有多量透明黄褐色漏出液,有的呈胶冻状凝块;心包膜上有奶酪样坏死,并与心脏粘连,肺鼓胀,表面覆有大量纤维素性渗出物,并与胸壁粘连,镜检肺泡充满水肿液     

对以纤维素性胸膜肺炎为主的猪呼吸道病的调查分析

正在解剖病死猪时常遇到以纤维素性胸膜肺炎为主要症状的呼吸道疾病。经笔者对170例具有纤维素性胸膜肺炎表现的病例进行病原培养、分离和鉴定,发现有75例是由副猪嗜血杆菌引起的,其他87例分离出了胸膜肺炎放线杆菌,有8例同时分离出了副猪嗜血杆菌和胸膜肺炎放线杆菌,下面我们把162例单纯感染病例的鉴别情况汇总如下。1疾病诊断采取支气管渗出物和肺炎病变组织,制成涂片和触片,革兰氏染色,用显微镜观     

天津地区副猪嗜血杆菌的分离鉴定及敏感药物筛选

为了给天津地区各区县养猪场提供副猪嗜血杆菌病的科学防控及合理的治疗用药方案,本试验对天津地区各区县养猪场疑似感染副猪嗜血杆菌的病猪进行了解剖及病理变化观察,无菌采集36份各猪场病猪组织脏器并进行细菌分离,对分离菌进行染色镜检、卫星试验、接触酶反应试验、微量生化反应和PCR鉴定。结果显示,剖检结果以纤维素性胸膜炎及腹膜炎常见,关节腔切面内有大量黄亮黏稠液体;分离出了9株疑似副猪嗜血杆菌菌株,染色镜检均可见革兰氏阴性多形态菌;分离菌有明显的卫星现象;接触酶反应试验均为阳性;微量生化反应D-核糖、山梨醇、果糖、阿拉伯糖和硫化氢均为阴性,麦芽糖、葡萄糖、蔗糖、甘露醇和脲酶均为阳性,山梨糖和木糖各有1株为阳性,5株菌β-半乳糖为阳性;PCR鉴定结果显示9株疑似副猪嗜血杆菌菌株在822 bp处均与阳性对照处于同一条带上,均判定为副猪嗜血杆菌,分离率达25%。用16种抗菌药物对9株副猪嗜血杆菌进行敏感药物筛选,药敏结果显示头孢曲松钠和氧氟沙星对分离株有良好的抑菌效果。本试验结果可为天津地区副猪嗜血杆菌病的科学防制提供依据。     

鸭大肠杆菌病的诊治

１　临床症状　病鸭精神沉郁,食欲减退或废绝,羽毛松乱,两翅下垂,闭目呈昏睡状,有的蹲地缩成一团。少数病鸭张口呼吸,排灰白、黄白或黄绿色稀粪,最后衰竭死亡。２　病理变化　心包膜呈黄白色、增厚,心包液增多,有的心包膜及胸膜壁相粘连。肺脏瘀血、水肿。肝脏瘀血、肿大,表面光滑,上覆一层白色的纤维素性渗出物,易剥离。肠道有卡他性或出血性炎症。３　实验室诊断　１－涂片镜检：肝脏触片,革兰氏染色,镜检,见两极着色较浓,有单个、散在、短小的革兰氏阴性杆菌。２－病原菌分离及其培养特性：按无菌操作法取肝、脾组织接种…     

副猪嗜血杆菌的分离鉴定及中药药敏试验

从数例疑似副猪嗜血杆菌病猪的肺脏和心包积液中分离到3株革兰氏阴性细小杆菌,经涂片镜检、生化试验和PCR鉴定,确定分离的3株菌为副猪嗜血杆菌.中药药敏试验结果表明,副猪嗜血杆菌对金银花及其组成的复方敏感,且复方作用效果优于单方.     

副猪嗜血杆菌病的诊治

<正>副猪嗜血杆菌病又称纤维素性浆膜炎和关节炎,为革兰氏阴性小杆菌,呈散发性的多发性浆膜炎和关节炎。这种细菌在环境中普遍存在,世界各地均有,健康猪群中也能发现该菌,初次感染的疫情相当严重。     

副猪嗜血杆菌病的流行特点和防治

一、副猪嗜血杆菌病流行特点副猪嗜血杆菌病是由副猪嗜血杆菌引起的一种以纤维素性浆膜炎、多发性关节炎,也称革拉瑟氏病。副猪嗜血杆菌属革兰氏阴性短小杆菌、形态多变。有报道显示,强毒力菌株一般呈短杆状。形态多变,有15个以上血清型,其中血     

副猪嗜血杆菌病的诊断及防制

副猪嗜血杆菌病,又称多发性纤维素性浆膜炎和关节炎,也称格拉泽氏病,由副猪嗜血杆菌引起。1病原学副猪嗜血杆菌为嗜血杆菌属中没有运动性的小型多形态杆菌,可见球杆状、长杆状及丝状等不同形态。革兰氏染色阴性,常见有荚膜,但多次体外培养或长时间培养（48小时以上）荚膜易消失。     

浅谈嗜血杆菌病的诊断与控制

副猪嗜血杆菌病又称纤维素性浆膜炎和关节炎,为革兰氏阴性小杆菌,呈散心性的多发性浆膜炎。近两年来。我国副嗜血杆菌在养猪场引起多发性浆膜和关节炎的报道屡见不鲜,特别是规模化猪场在受到蓝耳病、圆环病等感染之后免疫功能下降时,     

丹顶鹤大肠杆菌病的诊治

通过对某动物园6只发病死亡的丹顶鹤进行剖检，见3例呈纤维性心包炎变化，肝脏肿大且质地脆弱，脾有针尖大的出血点，取心、肝、脾等进行涂片，革兰氏染色，镜检，在各组织 均可见两端钝圆的革兰氏阴性杆菌。取心、肝、脾等脏器进行石蜡包埋，切片，HE染色，镜检。主要病理组织学变化为：心外膜增厚，有大量纤维蛋白及异嗜性白细胞，巨噬细胞浸润，部分心肌细胞，肝细胞，脾细胞发生凝固性坏死。取其肝，脾作电镜负染，未发现病毒颗粒。综合流行病学，病原学检查及病理变化等，可确诊为大肠杆菌感染。     

副猪嗜血杆菌人工感染猪的病理学观察

用副猪嗜血杆菌分离株感染断奶仔猪,发病仔猪临床表现为体温中度升高、精神不振、被毛粗乱、站立不稳、厌食、呼吸困难。尸体剖检,可见胸腹腔纤维素渗出严重,有大量的渗出液、肺出血及脑充血等;病理组织学表现为,肺脏、心脏、脾脏等脏器含有大量的中性粒细胞的纤维蛋白性多发性浆膜炎、脑膜炎及支气管肺炎。结果表明,此分离株为一高致病潜力的菌株。     

Detection of Haemophilus parasuis and Mycoplasma hyorhinis in swine and association of those pathogens with clinical and pathological-anatomic findings

In routine diagnostic it is difficult to detect a Haemophilus parasuis infection causing a polyserositis. In the presented study, a detection method by means of PCR, from collective swabs of the serosal surfaces from animals, with and without fibrinous serositis, was investigated. A significant association was detected between post mortal findings of fibrinous serositis and molecular biological detection of Haemophilus parasuis and Mycoplasma hyorhinis. However, no significant association existed between cultural detection of Haemophilus parasuis in bronchoalveolar lavage fluid and pathological-anatomical ascertainable fibrinous serositis. A significant association between Haemophilus parasuis and Mycoplasma hyorhinis could be determined in the animals that were included in this study.     

猪弓形虫自然感染病例病理组织学观察

为探究猪弓形虫感染对猪器官组织学结构的影响,对信阳某猪场自然发病猪进行病理学诊断。处死病猪后采取肝脏组织用福尔马林固定,姬姆萨染色法进行染色。采集肺、肝、肾、淋巴结和心等组织,石蜡包埋,HE染色,光学显微镜下观察。在用姬姆萨染色的肝脏切片上能够观察到弓形虫的速殖子,分别在肺、肝、肾、脾脏、淋巴结和心脏等组织器官切片中发现弓形虫滋养体或假囊,表明该场疾病是由猪弓形虫引起的,各组织器官出现出血、炎性细胞浸润、嗜酸性粒细胞和中性粒细胞增多等病理变化。     

副猪嗜血杆菌江西株的分离鉴定及药敏试验

从江西省彭泽县某猪场出现咳嗽、呼吸困难、胸膜有化脓性纤维蛋白渗出物病变的病死猪中分离到4株革兰氏阴性细小杆菌,对其进行培养特性和生化特性鉴定;用副猪嗜血杆菌16S rRNA的特异性PCR引物,通过PCR技术可从分离菌中扩增出821 bp的特异基因片段,表明该分离菌株为副猪嗜血杆菌。药敏试验结果表明,4株分离菌对头孢唑啉高度敏感,对头孢哌酮、氯霉素等敏感,而对复方新诺明、青霉素G等有抵抗力。小白鼠攻毒试验结果显示4株分离株均有致病性。     

Haemophilus parasuis: new trends on diagnosis, epidemiology and control

Haemophilus parasuis is a commensal organism of the upper respiratory tract of conventional pigs, but under appropriate conditions can invade and cause severe systemic disease, characterized by fibrinous polyserositis, arthritis and meningitis. Factors involved in systemic invasion by H. parasuis remain largely unknown. However, major advances in our knowledge of H. parasuis include (1) development of a species-specific PCR test to detect H. parasuis in clinical samples, (2) study of molecular epidemiology within and between herds, by use of a repetitive element-based PCR, (3) the proposal of an alternative serotyping technique, (4) development and testing of a new in vivo model for pathogenesis and virulence studies, and (5) use of controlled exposure of young pigs to low doses of live, virulent H. parasuis strains to reduce nursery mortality in affected swine herds.     

Isolation,Identification and Sensitive Drug Screening of Haemophilus parasuis in Tianjin

In order to provide a scientific prevention and treatment of Haemophilus parasuis (Hps) disease in pig farms of the Tianjin area, pigs with suspected Haemophilus parasuis disease were anatomized and observed pathological changes.The most common results were cellulose pleurisy and peritonitis, and there were more yellow viscous liquid in joint cavity section.The bacteria were isolated from 36 diseased pig swine organs which were suspected of Hps, and 9 strains of Hps were obtained through staining, satellite test, catalase reaction test, biochemical reaction and PCR.The 9 strains of Hps showed multi morphology and gram negative through staining; Satellite phenomenon was significant; All of the catalase reaction test were positive; D-ribose, sorbitol, fructose, arabinose and hydrogen sulfide of micro biochemical reaction were negative, but maltose, glucose, sucrose, mannitol and urease were positive, only 1 of 9 strains was positive sorbitol and xylose of micro biochemical reaction, 5 strains were positive for beta galactose;PCR result showed an objective fragments of 822 bp.The separation rate was 25%.The drug screening were done with 16 kinds of antimicrobial agents against 9 strains of Hps, drug sensitivity results showed that ceftriaxone sodium and levofloxacin had good antibacterial effects.The results of this study could provide the basis for prevention and control of Hps disease in Tianjin.     

副猪嗜血杆菌病流行病学及致病因子的研究进展

副猪嗜血杆菌（Haemophilus parasuis,Hps）是猪上呼吸道的一种常在菌,常引起猪的多发性浆膜炎、关节炎、脑膜炎,由该菌引起的病又称格拉泽氏病。随着世界养猪业的发展,该病已成为全球范围内影响养猪业的一种重要细菌性疾病。为了更全面的了解副猪嗜血杆菌病的研究现状,笔者对该病的病原学、流行病学、致病因子的研究进展作一综述。