Wet litter not only induces footpad dermatitis but also reduces overall welfare,technical performance,and carcass yield in broiler chickens

This study investigated whether a high level of footpad dermatitis (FPD) in broiler chickens induced by increased litter moisture content is accompanied by negative effects on technical performance, carcass yield, and other welfare aspects. Litter moisture content was increased by systematically spraying water over the litter from 6 d of age onward (lesion-induction treatment). Results were compared with a control group kept on relatively good quality litter and having a very low prevalence of footpad lesions. Litter quality significantly decreased from 7 d of age onward in the pens with the lesion-induction treatment compared with the control pens. At 21 and 36 d of age, significantly more FPD was observed in the lesion-induction groups compared with the control groups. Technical performance of the broilers was negatively affected for the lesion-induction groups from 28 d of age onward; lesion-induction groups had significantly lower BW gain, feed intake, and water intake and significantly higher FCR compared with control groups. As a result of the lower BW gain, carcass weight was less for the lesion-induction group and significantly more rejections for commercial parts were found. The lesion-induction groups also had significantly more hock burns and breast irritations at d 21 and 36, and were dirtier but had fewer thigh scratches at d 35 compared with the control groups. In addition, locomotion was negatively affected among the lesion-induction group, as they had a higher gait score at d 36 compared with the control group. We concluded that increased litter moisture content not only caused severe FPD but also reduced broiler performance and carcass yield and had a negative effect on other welfare aspects.     

Effect of hatch location and diet density on footpad dermatitis and growth performance in broiler chickens

The effect of hatch location and diet density on the prevalence of footpad dermatitis and growth performance in broiler chickens was studied. Broilers (Ross 308), incubated at 2 different hatch locations but originating from the same parent stock, were subjected to 2 feeding programs differing in energy content (2,750 vs. 2,950, 2,850 vs. 3,050, 2,900 vs. 3,100, and 2,900 vs. 3,100 kcal/kg for starter, grower I, grower II, and finisher diets, respectively) in a 2 × 2 factorial design (6 replicates per treatment combination). Broilers were housed under conditions and managed according to Dutch practice. Hatch location did not affect hatching results nor the prevalence and severity of footpad dermatitis, but did affect BW gain, feed, and water intake. A significant interaction was found between hatch location and feeding program; broilers fed the low-energy (LE) program had a better performance when hatched at Location 2 than at Location 1, whereas performance was similar for the high-energy (HE) broilers hatched at both locations. Broilers fed the LE program had similar BW gain but a higher feed conversion due to a higher feed intake as compared to broilers fed the HE program. In addition, moisture content of the litter in the pens with LE birds was higher than in pens with HE birds. As a result, broilers fed the LE program had more footpad dermatitis and hock burns at d 36 as compared to broilers fed the HE program. It is concluded that the HE feeding program is preferred to prevent footpad dermatitis and hock burn, and with respect to growth performance. The differences in growth performance between the 2 hatch locations merit further study but indicate the importance of the incubation and hatching environment and posthatch handling in relation to the growth performance of broilers on-farm.     

High dietary biotin levels affect the footpad and hock health of broiler chickens reared at different stocking densities and litter conditions

Responses to stocking density (SD), dietary biotin concentration and litter condition were evaluated on 2016 Ross 308 male broilers in the fattening period (day 22–day 42). The birds were placed in 48 pens with either dry or wet litter to simulate the final stocking density of 30 kg (12 broilers/m²; normal stocking density, NSD) and 40 kg (16 broilers/m²; high stocking density, HSD) of body weight (BW)/m² floor space. A corn–soybean meal‐based diet was supplemented with biotin to provide a normal (NB; 155 μg/kg) or high (HB, 1521 μg/kg) level of dietary biotin. There were six repetitions per treatment. The inappropriate moisture content of litter associated with HSD was avoided (p < 0.05) by good management (SD difference: dry litter, 6.65% vs. wet litter, 13.23%; 42 days), which made it advantageous (p < 0.01) for footpad (SD difference: dry litter, 0.118 vs. wet litter, 0.312; weekly average value) and hock health (SD difference: dry litter, 0.090 vs. wet litter, 0.303; weekly average value) of HSD birds, but not (p > 0.05) for growth and processing yield. In HSD, the biotin effect (gains, FCR) was significantly higher (p < 0.01) than in NSD. The similar response of HSD birds to supplemental biotin was observed (p < 0.05) for lesion scores of footpad and hock in particularly finishing chickens, and a significant interaction (p < 0.01) among stocking density, biotin supplementation and litter condition existed from 35 to 42 days of age. Taken together, increasing dietary biotin improves the performance and well‐being of broiler chickens stocked at high densities in litter‐independent and litter‐dependent manners respectively.     

Measurements of footpad dermatitis in broiler chickens at processing plants

A four-point photographic scale was developed to score the severity of lesions of footpad dermatitis (FPD) in broiler chickens. There was a linear relationship between the square root of the relative area of the lesions and their scores, and good agreement between different assessors using the scale. The scale was used to assess samples of 100 birds from each of 190 flocks slaughtered at two uk processing plants in 2002 and 2003; 12 of the flocks (6.3 per cent) had no lesions, but the others had lesions of different prevalences and severity. The maximum proportion of affected birds in a flock was 92 per cent. In the 178 affected flocks, 84.0 per cent of the birds had no lesions and 16.0 per cent had some evidence of FPD. The overall unweighted prevalence of birds with FPD in all 190 flocks sampled was 18.1 per cent; 10.2 per cent had only small lesions, on average equivalent in area to 2.1 per cent of the total area of the foot, 6.2 per cent had lesions on average equivalent to 6.6 per cent of the area of the foot, and 1.7 per cent had lesions on average equivalent to 21.5 per cent of the area of the foot. There were differences between the two plants in the overall prevalence and severity of FPD, but this may have been due to the fact that the plants were sampled at different times of the year.     

Practical assessment and management of foot pad dermatitis in commercial broiler chickens: A Field Study

Foot pad dermatitis (FPD) is a welfare concern in broiler chickens characterized by ulcerated lesions on the pad of the foot, which results from prolonged contact of foot pads with wet litter. During Canadian prairie winters, barn moisture levels tend to increase due to reduced ventilation as a means of conserving heat and minimizing costs. However, there are no published benchmarks regarding the prevalence of foot pad dermatitis in broilers reared in western Canadian provinces such as Alberta, Canada. As such the objectives of the current study were to evaluate practical means of assessing FPD in Alberta broilers as well as on-farm management practices which correlate with higher prevalence of foot pad dermatitis. A management-practices survey and 3 foot pad assessment methods were used to benchmark foot pad dermatitis in 32 broiler flocks throughout the province of Alberta. Four flocks per farm were sampled from a total of 8 commercial broiler farms. Per flock, 200 birds were assessed on-farm, 3 processor-line scores were taken at the processing plant, and 600 foot pad samples were assessed post-processing. The prevalence of foot pad dermatitis by assessment method was benchmarked on a per-flock basis at 28.65% on-farm, 26.17% on the processing line (processor-line), and 31.83% for samples taken off the processing line (processor-sampled). On-farm and processor-sampled assessment results were highly correlated (r = 0.90) compared with processor-line and on-farm (r = 0.77) and processor-line and processor-sampled results (r = 0.72; P < 0.001). Specifically, processor-line assessments were not found to be reliable when repeated (P > 0.10). On farm, wheat straw was used by the majority of Alberta’s producers (62.5%) and was associated with a higher prevalence of foot pad dermatitis per flock (40.6%). In contrast, pine shavings was associated with lower FPD prevalence (6.4%; P < 0.001), but was used by only 21.9% of producers in Alberta. Primary results from this field study support the use of on-farm FPD assessments rather than processor-line-based assessments, and a shift away from wheat straw as a broiler litter substrate.     

Toxicokinetics of the broad-spectrum pyrethroid insecticide deltamethrin in broiler chickens

1. The aim of this study was to examine single-dose toxicokinetics of deltamethrin, a broad-spectrum pyrethroid insecticide, for treatment of broiler chickens.

2. Twenty male broiler chickens were used. Animals were divided into two groups, each comprising 10 animals. An intravenous dose of 0.75 mg of deltamethrin/kg body weight was given intravenously to the first group and the same dose (0.75 mg/kg body weight) was administered by intracrop by gavage to the second group. Blood samples were also collected at specified intervals.

3. Serum deltamethrin levels were measured via micro-electron capture detection with gas chromatography equipment. According to the serum deltamethrin level-time curve, deltamethrin tended to distribute according to a two-compartment open model.

4. The half-life at β phase (t_1/2β), mean residence time (MRT) and area under the concentration time curve in 0-∞ (AUC_0→∞) values after intravenous application of deltamethrin were 4.00 ± 0.76 h, 4.65 ± 0.75 h and 702.27 ± 236.07 ng h/ml, respectively. Furthermore, the absorption half-life (t_1/2a), maximal concentration in serum after intracrop administration (C_max), time needed to reach C_max (t_max), t_1/2β, MRT and AUC_0→∞ values after intracrop application of deltamethrin were determined to be 0.18 ± 0.06 h, 19.65 ± 4.58 ng/ml, 0.70 ± 0.10 h, 7.27 ± 1.36 h, 10.46 ± 1.84 h and 153.33 ± 30.83 ng h/ml, respectively. The bioavailability of deltamethrin was 21.83%.

5. It was concluded that deltamethrin was rapidly but incompletely absorbed after intracrop administration and bioavailability was at a low level. The t_1/2β and MRT of the deltamethrin were short for both intracrop and intravenous applications, and the risk of toxic and residual effects of deltamethrin is therefore limited.     

Footpad dermatitis severity on turkey flocks and correlations with locomotion,litter conditions,and body weight at market age

Footpad dermatitis (FPD) and walking ability are both parameters used on poultry welfare audits. One survey was conducted to determine the correlation between FPD severity, gait score (GS), BW, and litter condition on turkeys at market age. A total of 41 commercial tom flocks of 16 to 19 wk of age were evaluated. On each visit, 50 toms were selected, weighed, and evaluated for GS and FPD. Litter score (LS) was also recorded according to moisture content and caking. Data analyses consisted of pairwise correlations and 3 separate multiple linear regression models using FPD, GS, and BW as response variables. Fixed effects included LS, season, FPD, GS, and BW. Based on pairwise correlations, FPD was correlated with LS, BW, GS, and age. Gait score was correlated with LS, BW, and age. Body weight was correlated with LS. With multiple linear regression analysis it was observed that LS and BW were the parameters that were associated the most with FPD severity and GS. As litter condition worsened and BW increased, FPD and GS worsened. Moreover, a seasonal effect was observed on both FPD and GS. Colder seasons, winter and autumn, were associated with increased FPD severity and GS. A negative effect of FPD and GS was observed on BW. In conclusion, FPD and GS are correlated with BW at market age and litter condition is a major factor in FPD development and walking ability in turkeys.     

Pre-caecal digestible phosphorus in maize and wheat for broiler chickens

1. The objective of this study was to determine the coefficient of pre-caecal digestion of P in maize (3.9 g/kg of total P, 0.83 g/kg of phytate P, 138 FTU [phytase units]/kg) and wheat (3.17 g/kg of total P, 1.94 g/kg of phytate P, 666 FTU/kg) in broilers according to the WPSA protocol.

2. For the diets, monosodium phosphate was used as an additional P supplement. Two sets of diets containing 200, 460 and 740 g/kg of wheat or 200, 500 and 740 g/kg of maize were formulated. A total of 288 21-d-old male broilers (Ross 308) were assigned to 24 cages (8 birds per cage) and the 6 test diets were assigned to cages. The coefficient of pre-caecal digestion of P was determined by the indicator method and linear regression.

3. In both ingredients, pre-caecal digestible P increased linearly with increasing inclusion levels of maize or wheat (P < 0.05). The coefficients of digestion of pre-caecal P were estimated to be 0.18 for wheat and 0.33 for maize.     

Differential expression of genes implicated in liver lipid metabolism in broiler chickens differing in weight

ABSTRACT

1. Lipid parameters and expression of ACACA, APOA1, CPT1A, FASN, FOXO1, LIPG, PPARα and SIRT1 genes involved in lipid metabolism were investigated in two groups of high (HW) and low (LW) weight broilers from the same strain.

2. Blood cholesterol and liver triglyceride levels were significantly increased in HW chickens compared to LW broilers, while other parameters, i.e. blood triglyceride, blood HDL/LDL, liver cholesterol and total liver fat showed no significant changes in either group.

3. The relative expression of ACACA, APOA1 and CPT1A genes was significantly lower in the liver tissues of HW broilers than in the LW group. The mRNA levels of these three genes showed a significant negative correlation with abdominal fat deposition and live weight of broilers. However, relative expression of FASN, FOXO1, LIPG, PPARα and SIRT1 hepatic genes did not differ among broilers.

4. It was concluded that, of eight hepatic genes implicated in lipid metabolism, only the expression of three (ACACA, APOA1 and CPT1A) were significant for fat and leanness within the same strain of chicken. Since reducing body fat is a major goal in the broiler industry, these data can provide fresh insight into the molecular processes underlying the regulation of fat deposition in broilers.     

Methionine+cystine requirement of broiler chickens fed low-density diets under tropical conditions

Two experiments were conducted to determine the M+C requirement of straight-run broiler chickens (Hubbard x Hubbard) during the period 4–21 (Exp. 1) and 21–40 (Exp. 2) days of age. Experiments were conducted during summer months (July–August) in open-sided houses, thus exposing chicks to chronic heat stress. Daily min-max temperature averaged 26–37C (Exp. 1) and 23–36C (Exp. 2). M+C deficient basal diets were formulated to contain low-nutrient-density, i.e., 2750 kcal per kg ME, 20.1% CP (Exp. 1), and 2780 kcal per kg ME, 17.0% CP (Exp. 2). Diets were supplemented with DL-methionine to provide total M+C level ranging from 0.64 to 0.89 % (six increments) and 0.54 to 0.79% (six increments), respectively in experiment 1 and 2. Requirements (0.95 of the maximum quadratic response) were found to be 0.77 and 0.75% total M+C, respectively for gain and feed efficiency, during 4–21 days; and 0.67% total M+C for both gain and feed efficiency during 21–40 days of age. Calculated on the digestible M+C basis, the estimates were 0.67 and 0.65% respectively for gain and feed efficiency during 4–21 days of age; and 0.60% for gain and feed efficiency during 21–40 days of age. Primary audience: Nutritionists, Formulators, Growers, and Poultry Researchers.     

Increased iron level in phytase-supplemented diets reduces performance and nutrient utilisation in broiler chickens

1. The effect of different levels of dietary iron on phytase activity and its subsequent effect on broiler performance were investigated in a 3 × 2 factorial arrangement. A total of 360 day-old Ross 308 male broiler chicks were distributed to 6 experimental diets, formulated with three levels of Fe (60, 80 and 100 mg/kg) and two levels of phytase (0 and 500 FTU/kg).

2. Phytase supplemented to mid-Fe diets increased feed consumption more than the non-supplemented diet at d 24. From hatch to d 35, Fe × phytase interaction significantly influenced the feed intake (FI), body weight gain (BWG) and feed conversion ratio (FCR). The high-Fe diet supplemented with phytase significantly reduced FI and BWG of broilers than those supplemented with low- or mid-Fe diets. The overall FCR was significantly better in birds fed on the mid-Fe diets with phytase supplementation.

3. A significant improvement in ileal digestibility of N, P, Mg and Fe was observed in birds feed diets containing 60 mg Fe/kg, with significant interaction between Fe and phytase.

4. Phytase improved the bone breaking strength when supplemented to low- or mid-Fe diets, compared to the non-supplemented diets. There was a significant Fe × phytase interaction effect. Tibia Fe content was higher in birds fed on phytase-free diets with high Fe but the reverse was the case when phytase was added and their interaction was significant. High dietary Fe significantly increased the accumulation of Fe in liver.

5. Phytase improved Ca-Mg-ATPase, Ca-ATPase and Mg-ATPase activities in jejunum when supplemented to the diet containing 80 mg Fe/kg.

6. This study indicates that high (100 mg/kg) dietary Fe inhibited phytase efficacy and subsequently reduced the overall performance and nutrient utilisation of broilers.     

Rearing conditions and foot-pad dermatitis in Swedish broiler chickens

A method of classification of broiler foot-health status was developed to estimate the prevalence of foot-pad dermatitis in Swedish broilers. Data on foot health were collected from 101 commercial broiler flocks at slaughter. The producers were asked to fill in a questionnaire on rearing conditions and equipment for every flock.

Lesions were commonly observed; only 62% of the birds were classified as being without lesions, 32% had mild lesions only (discoloration, erosions) and 6% had severe lesions (ulcers). Flocks reared in houses equipped with small water cups had significantly (P < 0.05) higher prevalences of foot-pad dermatitis than flocks reared with water nipples. Thick layers of litter, regardless of litter material, also resulted in significantly (P < 0.05) higher prevalences of foot-pad dermatitis than litter layers thinner than 5 cm.