Cardinal temperatures for germination and early growth of two Lesquerella species

Lesquerella (Lesquerella fendleri) is a potential alternative crop that is being studied for commercial oilseed production. Understanding the minimum temperatures for germination and seedling growth is important for determining potential areas for lesquerella production. The objectives of this study were to determine the cardinal temperatures for germination and seedling growth, and to screen ecotypes for germination and growth characteristics. A temperature gradient table arrangement was used to observe seed germination over a range of temperatures, and time to germination and shoot appearance. Times to 5 mm root length and 5 mm shoot length were also measured to assess cardinal temperatures for seedling survival and growth. Two different species were examined, L. fendleri and a species we refer to as ‘L. pallida aff.’ because it differed from typical L. pallida plants in chromosome number and in oil quality. We concluded that both germination and growth of L. pallida aff. occurred fastest at 22 °C, whereas L. fendleri germinated earlier at 18 °C, but grew faster at 22 °C. L. pallida aff. also had lower germination than L. fendleri over the range studied. Non-dormant seeds of improved lines of L. fendleri had better performance at temperatures above 22 °C than did unimproved accessions. Lines of L. fendleri selected for high oil content and salt tolerance had similar temperature requirements for germination except for improved line WCL-LO3, the current line being used in production. This line had optimal temperatures 6 °C higher for germination and growth than the other improved lines. Accessions of L. fendleri collected from elevations above 2000 m performed better at warmer temperatures, whereas those collected from elevations below 2000 m tended to perform better at cooler temperatures. Dormant seeds of L. fendleri germinated more quickly at low temperatures and had lower base (<3 °C) and optimal (22 °C) temperatures than non-dormant seeds (>7 °C and 28 °C, respectively). We speculate that this partial dormancy trait allows populations of L. fendleri to exploit a wider range of temperature conditions in the wild in order to thrive in extreme environments.     

Functional properties of protein from Lesquerella fendleri seed and press cake from oil processing

This investigation determined the functional properties of protein in Lesquerella fendleri seed and press cake from oil processing. L. fendleri seeds were heat-treated at 82 °C (180 °F) during 120 min residence time in the seed conditioner, and then screw-pressed to extract the oil. Unprocessed ground, defatted lesquerella seeds and press cakes were analyzed for proximate composition and protein functional properties. Protein from unprocessed lesquerella seed showed the greatest solubility (≥60%) at pH 2 and 10 and was least soluble (25%) at pH 5.5–7. Unprocessed lesquerella protein also had high surface hydrophobicity index (S_o), as well as, excellent foaming capacity and stability, emulsifying properties, and water-holding capacity (WHC) at pH 7. Protein solubility profile of the press cake showed up to 50% reduction in soluble proteins at nearly all pH levels, indicating heat denaturation during cooking and screw-pressing. Foaming capacity of the press cake protein decreased slightly, but foam stability was completely lost. Press cake protein also had markedly reduced values for S_o, emulsifying properties and WHC, further confirming lesquerella protein’s sensitivity to heat treatment.     

Oil analysis in seeds of Salicornia brachiata

Oil analysis in seeds of Salicornia brachiata was carried out in the current study. Hexane extraction yielded maximum oil content from seeds (22.4%). High ester (538.32 mg/g) and saponification (547.52 mg/g) suggest a potential for industrial use of the oil.     

Chemical composition and profile characteristics of Osage orange Maclura pomifera (Rafin.) Schneider seed and seed oil

Studies were conducted on the properties of seeds and oil extracted from Maclura pomifera seeds. The following values (on a dry-weight basis) were obtained for M. pomifera seed, respectively: moisture 5.88%, ash 6.72%, oil 32.75% and the high protein content 33.89%. The carbohydrate content (20.76%) can be regarded as a source of energy for animals if included in their diets. The major nutrients (mg/100 g oil) were: potassium (421.65), calcium (218.56) and magnesium (185.00). The physicochemical properties of the oil include: the saponification number 174.57; the iodine value 141.43; the p-anisidine value 1.86; the peroxide value 2.33 meq O₂/kg; the acid value 0.66; the carotenoid content 0.59 mg/100 g oil; the chlorophyll content 0.02 (mg/100 g oil) and the refractive index 1.45. Polymorphic changes were observed in thermal properties of M. pomifera seed oil. This showed absorbency in the UV-B and UV-C ranges with a potential for use as a broad spectrum UV protectant. The main fatty acids of the crude oil were linoleic (76.19%), oleic (13.87%), stearic (6.76%) and palmitic acid (2.40%). The polyunsaturated triacylglycerols (TAGs) LLL, PLL, POL + SLL, OLL, OOL (L: linoleic acid, O: oleic, P: palmitic acid and S: stearic acid) acids were the major TAGs found in M. pomifera seed oil. A relatively high level of sterols making up 852.93 mg/100 g seed oil was present. The sterol marker, β-sitosterol, accounted for 81% of the total sterol content in the seed oil and is followed by campesterol (7.4%), stigmasterol (4.2%), lupeol (4.1%) and Δ⁵-avenesterol (3.2%). The seed oil was rich in tocopherols with the following composition (mg/100 g): α-tocopherol 18.92; γ-tocopherol 10.80; β-tocopherol 6.02 and δ-tocopherol 6.29. The results showed that M. pomifera seed oil could be used in cosmetic, pharmaceutical and food products.     

Productivity and residual effects of legumes in rice-based cropping systems in a warm-temperate environment: I. Legume biomass production and N fixation

Field experiments were conducted to investigate the performance of temperate legume species in rice-based cropping systems in a warm-temperate environment in Nepal. Over the period 1994–1996, various legume species were grown during the winter season (October–May) in the Kathmandu valley (27° N, 1350 m asl) with the aim of evaluating their biomass production and N fixation. A wide range of legume species including food, feed and green manure crops proved to be very well adapted to the winter growing conditions in this environment. The cultivation of temperate legume crops therefore, constitutes an alternative to traditional cropping practices such as growing wheat or leaving the land fallow. The temperate species appeared to capitalise on generally favourable growing conditions such as long growing season, low pest and disease pressure, high radiant energy receipt and cool night temperatures. However, performance varied greatly between species and years. Total dry matter yields ranged from 2 to 20 t ha⁻¹ obtained with lentil (Lens culinaris Medic) and bitter lupin (Lupinus mutabilis), respectively. Highest seed yields were produced by fababean (Vicia faba) (5 t ha⁻¹) and field pea (Pisum sativum var. arvense) (3 t ha⁻¹) in the first season. Nitrogen yields and quantities of N fixed ranged from 18 to 481 kg ha⁻¹ and from 0 to 463 kg ha⁻¹, respectively. Large amounts of N were fixed by species such as fababean, Persian clover (Trifolium resupinatum) and bitter lupin. Early sowing in autumn was shown to be beneficial for some crops such as fababean, vetch (Vicia benghalensis) and Persian clover. In these cases, it is, therefore, important to reduce the turn-around interval after rice. Further research is required to fully determine the potential of temperate legume species in these environments with particular emphasis given to the identification of the most adapted cultivars and to reduce the need for irrigation of these winter crops.     

Maximizing yields in rice–groundnut cropping sequence through integrated nutrient management

Integrated use of organic and inorganic fertilizers can improve crop productivity and sustain soil health and fertility. The present research was conducted to study the effects of application of green manures [sesbania (Sesbania aculeate Poiret) and crotalaria (Crotalaria juncea L.)] and farmyard manure on productivity of rice (Oryza sativa L.) and its residual effects on subsequent groundnut (Arachis hypogaea L.) crop. Rice and groundnut crops were grown in sequence during rainy and post-rainy seasons with and without green manure in combination with different fertilizer and spacing treatments under irrigated conditions. The results showed that application of green manures sesbania and crotalaria at 10 t ha⁻¹ to rice compared to no green manure application significantly increased grain yield of rice by 1.6 and 1.1 t ha⁻¹, and pod yields of groundnut crop succeeding rice by 0.25 and 0.16 t ha⁻¹, respectively. There was no significant difference between the application of crotalaria or farmyard manure at 10 t ha⁻¹ on grain yields of rice, but pod yields of subsequent groundnut crop were greater with application of green manure. There was no significant effect of different spacing 20×15,15×15,15×10 cm² (333 000; 444 000; 666 000 plant ha⁻¹, respectively) on grain yield of rice. Pod yields of groundnut were significantly greater with closer spacing 15×15 cm² (444 000 plants ha⁻¹) as compared to spacing of 30×10 cm² (333 000 plants ha⁻¹). Maximum grain of rice was obtained by application of 120:26:37 kg NPK ha⁻¹ in combination with green manures, whereas maximum pod yield of groundnut was obtained by residual effect of green manure applied to rice and application of 30:26:33 kg NPK ha⁻¹ in combination with gypsum applied to groundnut crop.     

Radiation interception and the accumulation of biomass and nitrogen by soybean and three tropical annual forage legumes

Field experiments were conducted at Gatton and Dalby in southeastern Queensland to determine parameters associated with radiation interception and biomass and nitrogen (N) accumulation for the ley legume species, phasey bean (Macroptilum lathyroides (L.) Urban) and vigna, (Vigna trilobata (L.) Verdc.). Sesbania (Sesbania cannabina Retz.), a native legume species, and soybean (Glycine max (L.) Merrill)) were included in the study for comparison. The most important differences between species related to differences in radiation interception, radiation-use efficiency (RUE), N-accumulation efficiency and the partitioning of N to plant parts. During early growth, soybean intercepted more radiation than the other species, primarily because of its greater leaf area index (LAI). Sesbania had the highest RUE (1.08 g MJ⁻¹) followed by phasey bean (0.94 g MJ⁻¹), soybean (0.89 g MJ⁻¹) and vigna (0.77 g MJ⁻¹). The efficiency of N-accumulation was greater in soybean (0.028 g N g⁻¹) and phasey bean (0.030 g N g⁻¹) than in vigna (0.022 g N g⁻¹) and sesbania (0.021 g N g⁻¹). In all species, the proportion of N allocated to leaves declined throughout the experimental period, being more rapid in soybean than in sesbania and phasey bean. Despite this decline in total N partitioned to the leaves, both soybean and phasey bean maintained a relatively stable specific leaf nitrogen (SPLN) throughout the experimental periods although sesbania and vigna displayed rapid decreases in SPLN. The large variation between species in RUE and N-accumulation efficiency indicates that the development of ley legume cultivars with a combination of traits for more efficient legume production, water use and soil N-accumulation in the water-limited environments of the grain belt of eastern Australia may be possible. The sensitivity of forage production, water use and soil N-accumulation to variation in RUE and N-accumulation efficiency needs to be quantified using modeling techniques prior to embarking on screening programs to select appropriate germplasm for evaluation studies.     

Investigation of diacylglycerol acyltransferase (DGAT) activity of microsomes from the seeds of three euphorbs

Unusual fatty acids such as ricinoleate (12-hydroxyoleic acid) occurring in Ricinus communis L. or vernoleate (12,13-epoxyoleic acid) occurring in Euphorbia lagascae L. are suitable for industrial uses. Euphorbia lathyris L. is also a potential new oilseed crop on account of its high content of oleic acid in the seeds. The objective of this work was to test in vitro the preferences of E. lathyris microsomes for its native substrates (oleoyl-CoA and diolein) and to compare with R. communis and E. lagascae systems.The diacylglycerol acyltransferase (DGAT) catalyses the final step in transferring a fatty acid moiety to a diacylglycerol (DAG) into a triacylglycerol (TAG). To study the DGAT activity in microsomes of the three euphorbs: (1) plants of the three species were grown in a glasshouse at Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (Murcia, Spain), (2) endosperms were removed from developing seeds and the tissue was extracted, (3) in vitro DGAT assays using [¹⁴C]-oleoyl-CoA with or without 1,2-diolein were carried out and 4) labelled TAG were recorded using a molecular imager and a scintillation counter.Incorporation of [¹⁴C]-oleoyl into TAG was greater in R. communis and E. lathyris (72–89% of total TAG) than in E. lagascae. Adding exogenous 1,2-diolein (1 mM) to E. lagascae microsomes increased the amount of labelled TAG to 39%, suggesting that other acyl groups were being incorporated as well. R. communis and E. lagascae microsomes gave more-polar radiolabelled TAGs than E. lathyris possibly because endogenous DAGs (not 1,2-diolein) were being used in the reaction. Although E. lathyris microsomes showed specificity towards 1,2-diolein as a substrate, the preparations from R. communis, E. lagascae and E. lathyris were able to use the acyl donor and acyl receptor, possibly suggesting that DGAT enzymes would not be a limiting factor to engineer Euphorbiaceae crops with functionalized fatty acids.     

The efficiency of nitrogen fertiliser for rice in Bangladeshi farmers’ fields

Bangladesh is currently self sufficient in rice (Oryza sativa L.), which accounts for approximately 80% of the total cropped area, and 70% of the cost of crop production. However, farmers are increasingly concerned about the perceived decline in productivity, expressed as the return on fertiliser inputs. Agronomic efficiency is a measure of the increase in grain yield achieved per unit of fertiliser input that can provide a way to quantify the observation of farmers. This study indicates that the yields achieved where only P and K fertiliser were applied ranged from 3–5 t ha⁻¹, indicating good soil fertility, particular in terms of soil N supply (37–112 kg N ha⁻¹). However, at recommended rates and at rates used by farmers, the yield response to application of fertiliser N was low. Data shows that grain yields were significantly correlated in both years (R² = 0.77 and R² = 0.67) with plant uptake in nitrogen. The internal nitrogen use efficiency seems to confirm that sink formation was limited by factors other than nitrogen. Low agronomic efficiency (5–19 kg grain kg⁻¹ N) was caused by poor internal efficiency (45–73 kg grain kg⁻¹ N), rather than low supply of soil N or loss of fertiliser N. Thus, often the applications of large amounts of N fertiliser (39–175 kg N ha⁻¹) by farmers to increase yields of high yielding variety Boro rice were not justified agronomically and ecologically. A rate of 39 kg N ha⁻¹ is very low, hardly an environmental threat. No one single factor could be identified to explain the low internal efficiency. Therefore, it is concluded that the data presented tend to confirm the indication that yields are limited by a factor other than nitrogen, which could be crop establishment, plant density, water or pest management, micro-nutrients deficiency, poor seed and transplanted seedling quality, varieties and low radiation.     

Variation within a bread wheat cultivar for grain yield,protein content,carbon isotope discrimination and ash content

The study was undertaken to assess the variation within a bread wheat (Triticum aestivum L.) cultivar, primarily for grain yield, and the implications for wheat breeding. During the 1998–1999 growing season, cv. Nestos was established in a non-replicated (NR-0) honeycomb experiment, in the absence of competition (11 547 plants ha⁻¹). Ten high yielding (H) and 10 low yielding (L) plants were selected, the seeds of which were used to form the respective H and L lines. The 20 lines, along with their original cultivar, were evaluated in two locations either in the absence of competition (11 547 plants ha⁻¹) during the 1999–2000 season or under competition (5 000 000 plants ha⁻¹) during the 2000–2001 season. Results showed significant differentiation between lines for grain yield, determined both in the absence of competition at the single-plant level, i.e. yield per plant (YP), and under competition at the crop yield level, i.e. yield per plot (CY). Significant differences between lines were also found for grain protein content (PC), grain carbon isotope discrimination (Δ), and grain ash content (ASH), either in the absence of competition or under competition. A positive relationship was found between YP and CY (r=0.53,P<0.02). Results showed that selection within a bread wheat cultivar, under very low density and on the basis of individual plant grain yield, could be an effective way to either upgrade or maintain the cultivar, whereas the use of Δ or ASH as indirect selection criteria instead of grain yield was not supported by the study.     

Using legumes to enhance nitrogen fertility and improve soil condition in cotton cropping systems

Many Australian cotton growers now include legumes in their cropping system. Three experiments were conducted between 1994 and 1997 to evaluate the rotational effects of winter or summer legume crops grown either for grain or green manuring on following cotton (Gossypium hirsutum L.). Non-legume rotation crops, wheat (Triticum aestivum) and cotton, were included for comparison. Net nitrogen (N) balances, which included estimates of N associated with the nodulated roots, were calculated for the legume phase of each cropping sequence. Faba bean (Vicia faba — winter) fixed 135–244 kg N ha⁻¹ and soybean (Glycine max — summer) fixed 453–488 kg N ha⁻¹ and contributed up to 155 and 280 kg fixed N ha⁻¹, respectively, to the soil after seed harvest. Green-manured field pea (Pisum sativum — winter) and lablab (Lablab purpureus — summer) fixed 123–209 and 181–240 kg N ha⁻¹, respectively, before the crops were slashed and incorporated into the topsoil.In a separate experiment, the loss of N from ¹⁵N-labelled legume residues during the fallow between legume cropping and cotton sowing (5–6 months following summer crops and 9 months after winter crops) was between 9 and 40% of ¹⁵N added; in comparison, the loss of ¹⁵N fertilizer (urea) applied to the non-legume plots averaged 85% of ¹⁵N added. Little legume-derived ¹⁵N was lost from the system during the growth of the subsequent cotton crop.The improved N fertility of the legume-based systems was demonstrated by enhanced N uptake and lint yield of cotton. The economic optimum N fertilizer application rate was determined from the fitted N response curve observed following the application of N fertilizer at rates between 0 and 200 kg N ha⁻¹ (as anhydrous ammonia). Averaged over the three experiments, cotton following non-legume rotation crops required the application of 179 kg N ha⁻¹, whilst following the grain- and green-manured legume systems required only 90 and 52 kg N ha⁻¹, respectively.In addition to improvements in N availability, soil strength was generally lower following most legume crops than non-legume rotation crops. Penetrometer resistance during the growth of the subsequent cotton crop increased in the order faba bean, lablab, field pea, wheat, cotton, and soybean. It is speculated that reduced soil strength contributed to improvement in lint yields of the following cotton crops by facilitating the development of better root systems.     

Breeding for perennial growth and fertility in an Oryza sativa/O. longistaminata population

The development of perennial cultivars (CVs) of upland rice would give farmers a new tool to reduce soil erosion from hilly fields, thereby mitigating a problem of regional concern in Southeast Asia. Oryza longistaminata is an undomesticated, perennial, rhizomatous relative of domesticated Asian rice (Oryza sativa). Using five sets of 4 × 2 factorial mating designs, we crossed rhizomatous interspecific genotypes (IGs) from an intermated O. sativa/O. longistaminata population with male-fertile IG selections from the intermated population, and with O. sativa CVs. Parents and progeny were planted in an upland field at IRRI using a randomized complete block design and evaluated for rhizome expression, survival after 1 year, vigor of the survivors, and yield. For the IG parents, rhizome expression was variable and penetrance of most genotypes was incomplete, but genotypes that demonstrated the potential for moderate rhizome expression had high penetrance (89% average). The CV parents yielded 11.0 g/plant on average but none produced rhizomes or survived 1 year. The IG parents averaged yields of 3.1 g/plant, 57% rhizomatous and 36% survival. The IG/IG progeny averaged yields of 4.2 g/plant, 32% rhizomatous and 37% survival. The IG/CV progeny averaged yields of 6.0 g/plant, 18% rhizomatous and 16% survival. Nine IG/IG progeny and six IG/CV progeny were rhizomatous, perennial, and yielded at least 5 g/plant, and five of these yielded more than 10 g/plant. For the IG parents and IG/IG progeny, rhizome presence and expression were positively associated with survival and vigor of the survivors. General combining ability effects were significant for percent survival and yield but not percent rhizomatous. Specific combining ability effects were significant for percent rhizomatous, percent survival and yield. By selecting female parents for long rhizomes and male parents for fertility, considerable gains in rhizome expression, survival and yield were made. The development of perennial upland rice CVs should be feasible via introgression of genes from O. longistaminata.     

Anti-fungal action of bioconverted eicosapentaenoic acid (bEPA) against plant pathogens

Bioconverted eicosapentaenoic acid (bEPA), obtained from Pseudomonas aeruginosa PR3, was assessed for its in vitro and in vivo anti-fungal potential. Mycelial growth inhibition of the tested plant pathogens (Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Phyptophthora capsici, Sclerotinia sclerotiorum and Colletotrichum capsici) was measured in vitro. bEPA at the concentration of 5 μl/ml inhibited 52–60% fungal mycelial growth for all of the plant pathogens in vitro except S. sclerotiorum. Minimum inhibitory concentrations (MICs) of bEPA were found in the range of 250–500 μg/ml. Also, bEPA had a detrimental effect on spore germination for all the tested plant pathogens. Three plant pathogenic fungi (F. oxysporum, P. capsici and C. capsici) were subjected to an in vivo anti-fungal screening. bEPA at the initial concentration of 3000 μg/ml had a 100% anti-fungal effect against all of the tested plant pathogens. Concentrations of bEPA corresponding to 1500, 500 and 300 μg/ml were applied to the plants and revealed promising anti-fungal effects, supporting bEPA as a potential anti-fungal agent.     

Essential oil variation among and within six Achillea species transferred from different ecological regions in Iran to the field conditions

The compositions of essential oils of 19 accessions belonging to six different Achillea species, transferred from the natural habitats in 10 provinces of Iran to the field conditions, were assessed. The relationship between the leaf areas of selected accessions with their essential oil content was also investigated. Essential oil yield of dried plants obtained by hydro-distillation ranged from 0.1 to 2.7% in leaves. Results indicated a significant variation in oil composition among and within species. Total of 94 compounds were identified in 19 accessions belonging to the six species of A. millefolium, A. filipendulina, A. tenuifolia, A. santolina, A. biebersteinii and A. eriophora. The major constituents of the leaves in the tested genotypes were determined as germacrene-D, bicyclogermacrene, camphor, borneol, 1,8-cineole, spathulenol and bornyl acetate. According to the major compounds, four chemotypes were defined as: (I) spathulenol (1.64–34.31%) + camphor (0.2–15.61%) (7 accessions); (II₁) germacrene-D (18.78–23.93%) + borneol (7.93–8.26%) + bornyl acetate (11.56–14.66%) (5 accessions); (II₂) germacrene-D (13.28–36.28%) + bicyclogermacrene (5.93–8.4%) + 1,8-cineole (15.26–19.41%) + camphor (14.95–23.32%) (2 accessions); (III) borneol + camphor (52.04–63.27) (2 accessions); (IV) germacrene-D (45.86–69.64%) (3 accessions). The relationships of chemotypes with soil type and climatic conditions of collected regions were assessed, as probable reasons of high variations in essential oil components, and discussed.     

Comparison on the total phenol contents and the color of fresh and infrared dried olive leaves

The color (L*, a*, b* parameters), the total phenols content and the global chemical composition (moisture, protein, fat, carbohydrates and ash) of four fresh varieties of olive leaves (Chemlali, Chemchali, Zarrazi and Chetoui) were determined. Fresh olive leaves are characterized by a green color (greenness parameter, a*, varying from ?5.01 ± 0.26 to ?9.14 ± 1.21), an intermediate moisture content (0.85 to 1.00 g/g dry matter, i.e. 46 to 50 g/100 g fresh matter) and a variable amount of total phenols according to the olive leaf variety (from ≈2.32 to ≈1.40 g caffeic acid/100 g dry matter).Fresh leaves were submitted to blanching and/or infrared drying at 40, 50, 60 and 70 °C in order to be stabilized by reducing their moisture contents. The impact of IR drying temperature on some quality attributes (color, total phenols and moisture rate removal) was evaluated. Nevertheless, the effect of prior blanching treatment on the quality attributes of dried leaves is less significant and it depends on the olive leaf variety. The infrared drying induces a considerable moisture removal from the fresh leaves (more than 85%) and short drying durations (varying from ≈162 at 40 °C to 15 min at 70 °C). IR drying temperature showed a significant effect of on total phenols content and the color of the leaves whatever the leaf variety. In fact, total phenols content of dried olive leaves increased if compared to fresh ones. For example, total phenols of Chemlali leaves increased from 1.38 ± 0.02 (fresh leaves) to 2.13 ± 0.29 (dried at 40 °C) and to 5.14 ± 0.60 g caffeic acid/100 g dry matter (dried at 70 °C). IR drying allows preserving the greenness color of fresh leaves and enhancing their luminosity. It could be suggested for preserving olives leaves before their use in food or cosmetic applications.     

Water balance and rice growth responses to direct seeding,deep tillage,and landscape placement: Findings from a valley terrace in Nepal

For maximizing water retention and attaining high yields, transplanting into puddled soil (TPR) is often considered the optimal method of rice (Orzya sativa L.) establishment. Alternative management techniques like direct seeding (DSR) and deep tillage have been proposed as mechanisms to improve soil physical properties for subsequent dry-season crops, but the risks to rice are uncertain. In this full factorial study on a valley terrace in Nepal, the influence of tillage (shallow—T₁, deep chisel—T₂, deep chisel + moldboard plough—T₃) and establishment practice (TPR, DSR) on the field water balance and rice performance were evaluated in two adjacent landscape settings (terrace edge “upland”, central terrace “lowland”). Although deep tillage had only modest influences on seepage and percolation (SP) rates in both years (Y₁, Y₂), landscape placement and establishment practice had significant implications for the water balance (e.g. Y₂ SP cm day⁻¹: TPR-lowland = 1.6, DSR-lowland = 2.3, TPR-upland = 4.1, DSR-upland = 6.1). During low rainfall periods, however, soil water potential and drought vulnerability were governed solely by landscape placement. Despite water balance differences, there was little evidence that rice rooting behavior was substantially modified by landscape or establishment method. Weed biomass was higher in DSR, but was uncorrelated with water balance and productivity trends. In Y₁, lower SP rates and more days with continuous flooding were positively associated with rice productivity. DSR yields were significantly lower than TPR in both landscape positions, with the lowland outperforming the upland (Y₁ mt ha⁻¹: TPR-lowland = 6.4, DSR-lowland = 5.2, TPR-upland = 5.7, DSR-upland = 4.7). To determine if N dynamics were contributing to productivity differences, fertilizer nitrogen was increased from 120 to 150 kg N ha⁻¹ in Y₂. Results suggest that DSR performance is comparable – and landscape less important – if nitrogen is non-limiting (Y₂ mt ha⁻¹: TPR-lowland = 6.9, DSR-lowland = 6.5, TPR-upland = 7.0, DSR-upland = 6.5); no aspect of the field water balance was associated with yield variability in Y₂. For direct seeding in N-deficient farming systems, landscape criteria may prove useful for minimizing production risks by identifying field areas with lower SP rates.     

Hydrotime analysis of Lesquerella fendleri seed germination responses to priming treatments

Lesquerella fendleri seeds contain industrial oil, which is increased under arid environments. In such environments, the water needed for germination is available for only a short time, and consequently, successful crop establishment depends not only on rapid and uniform germination of the seedlot, but also on its ability to germinate under low water availability. All of these attributes can be analyzed through the hydrotime model (HT). Priming is a method to improve the speed and uniformity of germination. This technique reduces the hydrotime constant (θ_H) and sometimes displaces the base water potential (ψ_b(50)). This would increase the ability of the seed to germinate under low water availability.The objective of this work was to improve (i) the velocity and uniformity of germination and (ii) the ability to germinate under low water availability condition, in seeds of L. fendleri. We also intended to analyze and model changes in the physiological behaviour of the seedlot caused by the application of the priming treatment with seeds sown both in Petri dishes and containers with soil placed in the field.Seeds were subjected to priming in Petri dishes with a solution of polyethylene glycol (PEG) at 5 (P5) or 20 °C (P20). One-half of the seedlot was used for determination of hydrotime parameters by incubation in Petri dishes at 10, 20, and 30 °C, and in water or PEG solutions calibrated to obtain different ψ_a. The remaining one-half of the seedlot was sown in soil containers. Three different water availability treatments were imposed, field capacity or control, 75%, and 50% of the field capacity with the P5, P20, and untreated seeds. The P20 and P5 seeds germinated faster and more uniformly than the untreated (control) seeds under laboratory and field condition. The HT model analysis revealed that the θ_H constant had been reduced and ψ_b(50) had been shifted towards more negative values in both the P20 and P5 seedlots. This behaviour was consistent with the higher germination percentage attained by the P20 and P5 seeds in the field conditions under reduced water availability (i.e., 75 and 50% of field capacity) compared with that exhibited by the untreated seeds under the same situations.     

Interspecific competition,N use and interference with weeds in pea–barley intercropping

Field pea (Pisum sativum L.) and spring barley (Hordeum vulgare L.) were intercropped and sole cropped to compare the effects of crop diversity on productivity and use of N sources on a soil with a high weed pressure. ¹⁵N enrichment techniques were used to determine the pea–barley–weed-N dynamics. The pea–barley intercrop yielded 4.6 t grain ha⁻¹, which was significantly greater than the yields of pea and barley in sole cropping. Calculation of land equivalent ratios showed that plant growth factors were used from 25 to 38% more efficiently by the intercrop than by the sole crops. Barley sole crops accumulated 65 kg soil N ha⁻¹ in aboveground plant parts, which was similar to 73 kg soil N ha⁻¹ in the pea–barley intercrop and significantly greater than 15 kg soil N ha⁻¹ in the pea sole crop. The weeds accumulated 57 kg soil N ha⁻¹ in aboveground plant parts during the growing season in the pea sole crops. Intercropped barley accumulated 71 kg N ha⁻¹. Pea relied on N₂ fixation with 90–95% of aboveground N accumulation derived from N₂ fixation independent of cropping system. Pea grown in intercrop with barley instead of sole crop had greater competitive ability towards weeds and soil inorganic N was consequently used for barley grain production instead of weed biomass. There was no indication of a greater inorganic N content after pea compared to barley or pea–barley. However, 46 days after emergence there was about 30 kg N ha⁻¹ inorganic N more under the pea sole crop than under the other two crops. Such greater inorganic N levels during early growth phases was assumed to induce aggressive weed populations and interspecific competition. Pea–barley intercropping seems to be a promising practice of protein production in cropping systems with high weed pressures and low levels of available N.     

Intra-specific competition in maize: early establishment of hierarchies among plants affects final kernel set

Reduced plant biomass and increased plant-to-plant variability are expected responses to crowding in monocultures, but the underlying processes that control the onset of interplant interference and the establishment of hierarchies among plants within a stand are poorly understood. We tested the hypothesis that early determined plant types (i.e. dominant and dominated individuals) are the cause of the large variability in final kernel number per plant (KNP) usually observed at low values of plant growth rate (PGR) around silking in maize (Zea mays L.). Two hybrids (DK696 and Exp980) of contrasting response to crowding were cropped at different stand densities (6, 9 and 12 plants m⁻²), row spacings (0.35 and 0.70 m), and water regimes (rainfed and irrigated) during 1999/2000 and 2001/2002 in Argentina. The onset of interplant competition started very early during the cycle, and significant differences (P<0.05) in estimated plant biomass between stand densities were detected as soon as V_4–6 (DK696) and V_6–7 (Exp980). Plant population and row spacing treatments did not modify the onset of the hierarchical growth among plants, but did affect (P<0.02–0.08) the dynamic of the process. For both hybrids, the rate of change in relative growth between plant types was larger at 9 and 12 plants m⁻² (ca. 0.12 g/g per 100 °C day) than at 6 plants m⁻² (ca. 0.07 g/g per 100 °C day). For all treatments, the largest difference in estimated shoot biomass between plant types took place between 350 (V₇) and 750 °C day (V₁₃) from sowing, and remained constant from V₁₃ onwards. Dominant plants always had more kernels per plant (P<0.05) than the dominated ones, but differences between plant types in PGR around silking were significant (P<0.05) only at 12 plants m⁻². Our research confirmed the significant (P<0.01) curvilinear response of KNP to PGR around silking, but also determined a differential response between plant types: the mean of residual values were significantly (P<0.01) larger for dominant than for dominated individuals. Estimated ear biomass at the onset of active kernel growth (R₃) reflected the variation in KNP (r²≥0.62), and was significantly (P<0.01) related to estimated plant biomass at the start of active ear growth (ca. V₁₃). This response suggested that the physiological state of each plant at the beginning of the critical period had conditioned its reproductive fate. This early effect of plant type on final KNP seemed to be exerted through current assimilate partitioning during the critical period.     

Long-term effects of inorganic and organic fertilizer sources on yield and nutrient accumulation of lowland rice

Deceleration in rice (Oryza sativa L.) yield over time under fixed management conditions is a concern for countries like Bangladesh, where rice is the primary source of calories for the human population. Field experiments were conducted from 1990 to 1999 on a Chhiata clay loam soil (Hyperthermic Vertic Endoaquept) in Bangladesh, to determine the effect of different doses of chemical fertilizers alone or in combination with cow dung (CD) and rice husk ash (ash) on yield of lowland rice. Two rice crops—dry season rice (December–May) and wet season rice (July–November) were grown in each year. Six treatments—absolute control (T₁), one-third of recommended fertilizer doses (T₂), two-thirds of recommended fertilizer doses (T₃), full doses of recommended fertilizers (T₄), T₂+5 t CD and 2.5 t ash ha⁻¹ (T₅) and T₃+5 t CD and 2.5 t ash ha⁻¹ (T₆) were compared. The CD and ash were applied on dry season rice only. The 10-year mean grain yield of rice with T₁ was 5.33 t ha⁻¹ per year, while the yield with T₂ was 6.86 t ha⁻¹ per year. Increased fertilizer doses with T₃ increased the grain yield to 8.07 t ha⁻¹ per year, while the application of recommended chemical fertilizer doses (T₄) gave 8.87 t ha⁻¹ per year. The application of CD and ash (T₅ and T₆) increased rice yield by about 1 t ha⁻¹ per year over that obtained with chemical fertilizer alone (T₂ and T₃, respectively). Over 10 years, the grain yield trend with the control plots was negative, but not significantly, both in the dry and wet seasons. Under T₃ through T₆, the yield trend was significantly positive in the dry season, but no significant trend was observed in the wet season. The treatments, which showed positive yield trend, also showed positive total P uptake trend. Positive yield trends were attributed to the increasing P supplying power of the soil.