Predatory phytoseiid mites (Acari: Mesostigmata) in vineyards and fruit orchards in Southern Tyrol

Results of monitoring communities of the predatory phytoseiid mites in 70 commercial vineyards and 29 apple orchards in the Eisack-and Etsch-river valleys, Southern Tyrol, are reported and discussed.Typhlodromus pyri Scheuten,Amblyseius andersoni (Chant) and, locally,A. finlandicus Oudemans were the most abundant species.     

Distribution of the predatory mite <Emphasis Type="Italic">Amblyseius andersoni</Emphasis> Chant (Acari: Phytoseiidae) on different apple cultivars

The effect of apple cultivar on the distribution of Amblyseius andersoni was studied in an experimental orchard where tetranychids were virtually absent and eriophyids reached low to moderate populations. Seven apple scab-resistant cultivars, showing some differences in their leaf morphology, were compared. Each cultivar was grafted onto three different rootstocks inducing increasing vigor. The intraplant distribution of A. andersoni was also studied by monitoring predator densities on basal and apical leaves. In the first year, the colonization of different cultivars by A. andersoni showed definite patterns, sometimes associated with the abundance of the eriophyid Aculus schlechtendali. In the second experimental season, eriophyids reached negligible levels and the effect of cultivar on A. andersoni abundance was less clear. Florina and N.Y. 18?491 cultivars were colonized in both years by relatively high phytoseiid numbers, while TSR 29T219 and Priscilla showed low populations in at least one season. The results observed on the remaining cultivars were intermediate or indefinite. The role of leaf pubescence on phytoseiid colonization is discussed.     

Side effects of botanical insecticides on predatory mite Amblyseius andersoni (Chant)

The effects of the botanical insecticides Biopiren plus, Piresan plus and Neemazal T/S on the predatory mite Amblyseius andersoni (Chant) were tested under laboratory, semi-field, and field conditions. The tests carried out in the laboratory allowed detection of the direct toxicity on eggs and females as well as the effects on fecundity, whereas semi-field trials assessed their residual toxicity. In laboratory and semi-field tests, pyrethrins, particularly Piresan plus, proved to have the highest toxicity with E value, concerning both female mortality and fecundity, of 100?% and 45?%, respectively. The same product tested in the field only caused a significant reduction in the phytoseiid population shortly after the treatment. The relevant escape rate registered in semi-field tests could explain the lack of phytoseiid eradication in the field after treatment with pyrethrins.     

Copulation, egg production and sex ratio inCydnodromella negevi andTyphlodromus athiasae (Acari, Phytoseiidae)

The effect of the duration of copulation on egg production and sex ratio was studied in two phytoseiid mites,Cydnodromella negevi (Swirski and Amitai) andTyphlodromus athiasae Porath and Swirski in the laboratory. In both species, the fecundity of females, oviposition period and the proportion of females in the progeny increased with the duration of copulation. It was shown that in the two physoseiid mites several copulations are required to deposit the maximum number of eggs.     

Side effect of some fungicides on the mite population in an apple orchard in Croatia

The research into the side effect of fungicides used in the control of the most serious disease in apples (Venturia inaequalis) i. e. the research into the influence of fungicides on the population of phytophagous mites, was carried out at Nedeli??e (North-West Croatia) during 1997 and 1998. The study is related to the population of the mites most present in apple orchards, such as red spider mite (Pananychus ulmi) and other mites (Aculus schlechtendali andTetranychus urticae). Fungicides which are known to be effective against scab, i. e.Venturia inaequalis, and used in fruit production in Croatia, were tested in order to investigate their secondary properties which have not yet been studied. The following fungicides were used: cyprodinil, dodine, dichlofluanid + bitertanol (as tank mix), and kresoxim-methyl. The tests were repeated three times; the application time was determined by monitoring the conditions for infection. The treatments were done curatively, i. e. up to 96 h after the onset of infection conditions. The mite population was estimated by the pressing method, which yielded the average number of mites per leaf. All the results were statistically processed by variance analysis and by Duncan’s Multiple Range Test, and are presented graphically and in tables. The paper also presents graphically the climatic conditions, scab infections and application time. The research over two years have shown a pronounced suppressive effect of dichlofluanid + bitertanol on populations of phytophagous mites, while the other fungicides used in the research had a neutral effect on the mite papulation.     

Evaluation of Pinus radiata seed treatments to control Fusarium circinatum: effects on seed emergence and disease incidence

In this study, the effects of hot water (HWT), hydrogen peroxide and fungicides on the incidence of Fusarium circinatum on artificially inoculated Pinus radiata seeds were evaluated. Fifteen commercial fungicide formulations were screened in vitro for inhibitory activity on mycelial growth and conidial germination of F. circinatum. With half‐maximal effective concentration (EC₅₀) lower than 0.5 ppm, fluazinam, imazalil and tebuconazole were the most effective fungicides on mycelial growth, while captan, mancozeb or pyraclostrobin were the most effective (EC₅₀ < 0.3 ppm) on conidial germination. Based on the results obtained, imazalil, fluazinam, mancozeb and pyraclostrobin were selected for further testing. The effects of HWT, hydrogen peroxide and fungicide treatments on seed emergence and the incidence of F. circinatum were assessed. Seed treatments with fungicides prior to sowing were less effective and inconsistent in reducing the incidence of F. circinatum on seedlings. In contrast, hot water and hydrogen peroxide treatments significantly reduced F. circinatum contamination on P. radiata seeds with an overall disease incidence lower than 0.8% on seedlings. Furthermore, subsequent application of fungicides on seedlings did not improve the effectiveness of HWT. These results, therefore, suggest that hot water is a better alternative to hydrogen peroxide and fungicides as Pinus seed treatment against F. circinatum and could easily be implemented as standard in commercial nurseries to control the spread of the pitch canker disease.     

5种药剂防治核桃膏药病药效试验

2013年10～12月,采用松脂酸钠、松脂酸铜、噻霉酮、代森锰锌等5种药剂对核桃膏药病进行防治试验。结果表明,喷施1600倍液的松脂酸钠、松脂酸铜、噻霉酮、代森锰锌,对核桃膏药病的防治效果分别为44.0％、19.7％、36.6％和29.5％;而喷施其800倍液,防治效果分别为76.9％～80.7％、13.3％～20.7％、65.1％～78.3％和67.4％～77.5％。松脂酸钠、噻霉酮、代森锰锌等3种药剂对核桃膏药病具有较好的防治效果,尤以松脂酸钠防治效果最好。涂白剂对核桃膏药病具有一定的防治效果,而松脂酸铜的防治效果不明显。     

Efficacy of fungicides on Hymenoscyphus fraxineus and their potential for control of ash dieback in forest nurseries

Twenty‐six commercial formulations of fungicides at six concentrations were evaluated in vitro for their efficacy on mycelial growth of Hymenoscyphus fraxineus (anamorph Chalara fraxinea). The results are presented as EC₅₀, EC₉₀ and minimal inhibitory concentration values; the comparisons with the recommended application concentrations showed that 10 of the 26 fungicides were highly effective in their ability to inhibit the mycelial growth of the pathogen. The eight most effective fungicides identified based on multiple comparisons analysis were azoxystrobin, bitertanol, captan (in two‐component preparation with trifloxystrobin), difenoconazole, kresoxim‐methyl, spiroxamine (in multicomponent preparation with tebuconazole and triadimenol), tebuconazole and trifloxystrobin. Azoxystrobin, difenoconazole, kresoxim‐methyl, mancozeb, myclobutanil, pyrimethanil, tebuconazole and trifloxystrobin were selected to verify their effectiveness in in planta tests. During two field tests in nurseries, it was found out that tebuconazole (triazole), trifloxystrobin and kresoxim‐methyl (strobilurins) and mancozeb (dithiocarbamate) were significantly effective against H. fraxineus. The best results were gained with tebuconazole, when mean percentage of diseased saplings was 16.3%, whereas in the control, it was 63.6%. The combination and alternation of fungicides from the triazole, strobilurine and dithiocarbamate chemical groups should be an effective tool for protecting ash saplings in forest nurseries. Moreover, exact timing of the treatment reflecting rainfall and development of first symptoms in the foliage will be also important.     

Harmfulness of eriophyid mites (Eriophyoidea,Acari) causing galls on trees and shrubs in the Czech Republic

Fifteen eriophyid mite species are the most abundant species in the Czech Republic from which four, viz.Eriophyes fraxinivorus onFraxinus excelsior, E. platanoideus onAcer platanoides, Phytoptus pyri onPyrus communis andStenacis triradiatus on various species ofSalix injure their host plants and are evaluated as pests. The weight of the leaves attacked by eriophyid mites increase with increasing number of galls pro leaf but the surface of attacked leaves decrease with increasing attack. Harmfulness of eriophyid mites manifests by reducing of assimilation processes, retardation of growth, precocious drying and fall-off of attacked leaves. Heavily attacked young shrubs and trees may dry and decease.With one figure and 2 tables     

Use of standard toxicity tests with Typhlodromus pyri and Aphidius rhopalosiphi to establish a dose-response relationship

The existing standardised test systems for assessing the toxicity of crop protection products to the non-target arthropods Typhlodromus pyri (Acari: Phytoseiidae) and Aphidius rhopalosiphi (Hymenoptera: Aphidiidae) are limit tests designed to compare a single-use rate of the product with a water control. The suitability of these test systems for generating dose-response data as required for refined ecotoxicological risk assessment was evaluated. Data on dose-response toxicity of crop protection products to T. pyri and A. rhopalosiphi were generated under worst-case laboratory and to T. pyri under extended laboratory conditions and analysed using the standard Probit method, a logistic regression, a generalised Probit analysis, and the moving average-angle method in order to calculate the LR₅₀-values (application rate killing 50?% of the exposed organisms). The fit of the models, the precision of the resulting LR₅₀ values, and the required minimum number of replicates were compared. In 85?% of the studies, at least one of the statistical methods led to satisfactory results. The moving average-angle method was the most widely applicable method. The results show that the existing guidelines can be used to perform dose-response tests. Implications for risk assessment are discussed.     

Versuche über die Empfindlichkeit vonTrichogramma (Hymenoptera,Chalcidoidea) gegenüber Fungiziden

Zusammenfassung Die Wirkung von 12 im Obstbau gebräuchlichen Fungiziden in praxisüblichen Anwendungskonzentrationen aufTrichogramma-Imagines wurde in Laborversuchen geprüft.Alle berücksichtigten Mehltau-Bekämpfungsmittel, Binapacryl, Dinocap, Triamphos und Chinomethionat, erwiesen sich als mehr oder weniger toxisch, desgleichen die Schorf-Bekämpfungsmittel Mancozeb, Dichlofluanid, Metiram und TMTD. Für die Schorf-Bekämpfungsmittel Captan, Dithianon, Dodin und Zineb wurde unter den Versuchsbedingungen keine Giftwirkung nachgewiesen.Die Angaben der Literatur über die Wirkung der genannten Fungizide aufTrichogramma und andere Chalcidoidea werden diskutiert.

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Summary The effects uponTrichogramma adults of 12 fungicides which are commonly in use in orchards, in normal concentrations were examined by laboratory trials.The mildew fungicides, binapacryl, dinocap, triamphos, and oxythioquinox proved to be toxic as well as the apple scab fungicides mancozeb (= coordination product of zinc ion and [ethylenebis(dithiocarbamato)] manganese), dichlofluanid, metiram (= zinc-activated polyethylene thiuram disulfide), and thiram. Under the conditions of the experiments, there was no evidence of a toxic effect exerted by the apple scab fungicides captan, dithianon, dodine, and zineb.The available data from literature on the effects of the fungicides mentioned uponTrichogramma and other chalcidoids are discussed.

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Durchgeführt mit Unterstützung der Deutschen Forschungsgemeinschaft.

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Dem Direktor des Instituts, Herrn Professor Dr. J. M. Franz, danke ich für sein reges Interesse an den Versuchen und die mir jederzeit gewährte Unterstützung.     

Seasonal population dynamics of Typhlodromus pyri Scheuten (Acari, Phytoseiidae) in apple orchards in the region Meckenheim

Typhlodromus pyri Scheuten (Acari, Phytoseiidae) has been reported as the most important predator of the European red mite, Panonychus ulmi (Koch) (Acari, Tetranychidae) in apple culture. However, biological data on T. pyri was insufficient on important parameters like seasonal population dynamics and within-tree and on-leaf distribution in apple orchards in the region of Meckenheim, Germany. The purpose of the present work was, therefore, to obtain experimental data on these parameters of T. pyri from 2000 to 2002 in the apple orchards in this region. The results showed that overwintered females of T. pyri reactivate and start laying eggs between late March and early April. The mean daily number of the different stages of T. pyri per leaf peaked in July each year, where it ranged between 0.92–1.68 eggs, 0.81–1.41 nymphs, 0.64–1.32 females and 0.37–0.52 males. A significantly higher number of T. pyri individuals congregated in the bottom layer of trees (0.34–0.64 individuals/leaf) than in the middle and top layers (0.23–0.38 and 0.12–0.23 individuals/leaf, respectively). We found that 91.4–94.1% of the total eggs, 89.6–91.7% larvae, 73.0–76.5% nymphs, 60.5–64.6% adult females and 52.6–55.9% adult males concentrated their activities in the inner area of the leaf surface. More than 50% of the predatory mites congregated in the bottom layer in winter. Mean total density of the overwintering females in December of 2000,2001 and 2002 ranged between 10.6–14.2 females/5 cm twig in the bottom, 7.3–10.4 in the middle and 4.1–6.9 in the top layers of the apple trees. Mean total fecundity recorded over the 3 years was lowest in the overwintering generation with 31.8–37.6 eggs/female, highest in the June generation with 45.9–53.7 eggs/female, and intermediate in the August generation with 41.5–46.1 eggs/female. The overwintering generation was composed entirely of females, while the female to male ratio in the June generation was 2:1, and in the August generation 3:1. T. pyri overwinters as adult females in rough structures, cracks and crevices of twigs.     

Prey consumption during development as well as longevity and reproduction of <Emphasis Type="Italic">Typhlodromus pyri</Emphasis> Scheuten (Acari,Phytoseiidae) at higher temperatures in the laboratory

The predatory mite Typhlodromus pyri Scheuten (Acari, Phytoseiidae) has been reported as an important predator of the European red mite, Panonychus ulmi (Koch) (Acari, Tetranychidae) in apple culture and vineyards at below 25?°C. However, sufficient biological data was lacking on its efficiency at temperatures above 25?°C. Therefore, the purpose of the present laboratory work was to obtain experimental data on prey consumption during development as well as longevity and reproduction of T. pyri on apple leaf discs and in Plexiglas cells at constant temperatures of 25?±?2?°C and 30?±?2?°C with P. ulmi as prey.     

核桃膏药病化学防治试验

为筛选出对核桃膏药病防效较好的药剂,进行子代森锰锌、噻霉酮、松脂酸钠3种药剂防治核桃膏药病的试验,结果表明:在核桃树膏药病发病初期,分别使用代森锰锌、噻霉酮、松脂酸钠800倍稀释液对核桃树进行整株喷施,对核桃膏药病的防治效果分别为70.5%、65.9%、77.5%。试验使用的3种药剂对核桃膏药病都表现出了较好的防治效果,其中,松脂酸钠的防效最好,代森锰锌的防治效果次之,噻霉酮在3种试验药剂中防效最低。     

Development and population growth of <Emphasis Type="Italic">Stephanitis pyri</Emphasis> (F.) (Heteroptera: Tingidae) at five temperatures

Pear lace bug, Stephanitis pyri (F.) (Heteroptera: Tingidae) is a pest of apple and pear trees and ornamental Rosaceae plants in Mediterranean countries and palearctic region. The aim of this study is to determine the effects of temperatures on S. pyri in the laboratory. Development and fecundity of S. pyri reared on apple leaves (Pyrus malus L.) were investigated at five constant temperatures (20, 23, 26, 29 and 32 ± 1°C) and a 16L:8D photoperiod. Longevity was determined to be 12.6 days at 32°C and 58.7 days at 20°C for females, and 9.7 and 37.7 days for males. Females laid 186.9 eggs per female with the highest number achieved during 28.5 days of oviposition period at 26°C. Female lifetime fecundity was reduced at 32°C (40.0 eggs per female). While the net reproductive rate (R ₀) was highest at 26°C, the intrinsic rate of natural increase (r _m ) was highest at both 26 and 32°C. The mean generation time (G) was estimated to be 27.2–78.4 days at 20 and 32°C, respectively. The longest development times for egg and total nymph stages were obtained as 22.0 and 24.9 days, respectively, at 20°C. S. pyri developed fastest from egg to egg in 24.3 days at 32°C. The lower developmental threshold (T ₀) was 9.7°C and the thermal constant (K) was 517.3 degree-days for S. pyri. Thus, S. pyri is calculated to have 3.8 theoretical generations in Tekirdag. The optimum developmental temperature for S. pyri was 26°C.     

A hazard quotient approach for assessing the risk to non-target arthropods from

The EU Plant Protection Product Directive 91/414/EEC recommends the EPPO/CoE Arthropod Natural Enemies Risk Assessment Scheme for guidance on how to conduct risk assessments for terrestrial non-target arthropods. This scheme is currently in the process of being revised by EPPO/ CoE. A major change will be the recommendation for the generation and use of ’Dose Response’ toxicity data instead of limit test data. In addition, the revised EPPO/CoE Non-target Arthropods Risk Assessment Scheme will replace the current arbitrary 30% threshold trigger value applied to limit test data, with a Hazard Quotient (HQ; = Ratio Application Rate/LC50 on Glass)), comparable to the successful approach adopted in the EPPO/CoE ’Honeybee Risk Assessment Scheme’. However, in order for this new approach to be implemented under 91/414/EEC, an appropriate regulatory HQ trigger value needs to be derived. Such an HQ trigger value has been established by calculating HQ values for the 2 recommended sensitive indicator species (T pyri andAphidius) for a wide range of products and validating opposite robust semi-field/field data. This validation indicated that an HQ trigger value of ≥ 12 forT pyri and ≥ 8 forAphidius spp., should be used to trigger higher-tier risk assessment and/or higher-tier testing for non-target arthropods. As these trigger values were validated with realistic semi-field/ field data they apply for both lethal and sub-lethal effects as well as single and multiple application scenarios. Due to the worst case assumptions used in this HQ validation analysis, no further uncertainty factors need to be applied for in-crop risk assessment. Whilst a small amount of uncertainty exists regarding the comparative sensitivity ofT pyri andAphidius spp. for off-crop non-target arthropod guilds of arthropods, this is balanced by the fact that the off-crop exposure assessment used in the HQ derivation, is at least an order of magnitude higher than that realistically likely in the field. This HQ approach and trigger value is an appropriate and conservative tool for tier 1 risk assessment, which should reduce the number of false positive results leading to unnecessary higher-tier testing.     

杨树壳梭孢溃疡病菌的室内毒力测定

在室内培养条件下,采用生长速率法测定了6种常用杀菌剂对杨树壳梭孢溃疡病菌Fusicco-cum fesculi的抑制效果,结果表明:80%代森锰锌WP和50%异菌脲WP对F.fesculi抑制效果最好,EC50值分别是38.175,41.947 mg/L;70%甲基硫菌灵WP2,5%三唑酮WP和75%百菌清WP抑菌效果次之,EC50值分别是137.43,149.90,205.98 mg/L;60%多菌灵WP抑菌效果最差,EC50值是419.91 mg/L。     

Cannibalism and interspecific predation of the phytoseiid mite,<Emphasis Type="Italic"> Amblyseius swirskii</Emphasis>

A laboratory study revealed that females of the phytoseiid mite, Amblyseius swirskii Athias-Henriot, fed on protonymphs of their own species or of other predatory mites i.e. Agistemus exsertus Gonzales and Euseius scutalis Athias-Henriot exhibited a marked decline in their fecundity and a shortening in their longevity. Moreover, the rate of predation was also reduced. When A. swirskii females were fed on nymphs of the two-spotted spider mite Tetranychus urticae Koch after having been fed previously on protonymphs of either A. swirskii or A. exsertus increases in the rates of oviposition and consumption were observed, although these rates were lower than those of conspecific females reared continuously on the two-spotted spider mite only.     

Survey of predacious mites on citrus in South Africa. Specific diversity, geographic distribution and the abundance of predacious mites

During a survey (March–June, 1995) of predacious mites on citrus in 30 major production areas, 25 species of phytoseiid and Anystid mites were recorded.Amblyseius pafuriensis Van der Merwe occurred almost at every sites in the Northern Transvaal and Southern Zimbabwe,A. citri (Van cer Merwe & Ryke) was most common in Central and Eastern Transvaal,A. citri andTyphlodromus crassus Van der Merwe, Natal, andA. addoensis addoensis (Van der Merwe & Ryke) andA. addoensis rubicolus (Van der Merwe & Ryke) in both Cape provinces. Regarding the whole region,A. addoensis addoensis, A. citri, A. addoensis rubicolus represented 86% of the total number of sampled predators,A. pafuriensis, T. crassus andT. rasilis Van der Merwe together represented 5% and the remaining 20 species represented 9%. The study indicated thatA. pafuriensis, T. crassus andT. rasilis can be excellent biocontrol agents on citrus.     

Elm bark beetles and Dutch Elm Disease: tests of combined control

The possibility for the combined control of Ophiostoma novo-ulmi and Scolytus multistriatus was tested in Italy. Two elm clones [U. pumila × U. minor (clone B) and U. glabra var. pendula grafted onto U. pumila × U. minor pollards (clone A)] were treated by stem injection of different blend or concentrations of fungicides and insecticides. Then, adults of S. multistriatus, either loaded with spores of two isolates of O. novo-ulmi (H328 and 182) or provided of their natural load of conidia, were forced to feed in twig crotches of the treated trees. After 48 hours all insects were checked (dead or alive). One month later, the same twigs were cut off and brought to the laboratory. From each twig, three discs almost 1?mm thick were then taken (0, 3 and 6?cm over the point where the insect fed). The wood discs were inoculated in selective substrate and stored until the development of O.?novo-ulmi colonies. All thesis containing insecticides give good results against S. multistriatus. Similarly, all thesis containing fungicides show a good fungus control independently from the fungal isolated. The percentage of fungal transmission obtained from insects carrying the natural load of conidia was lower than percentages obtained from beetles artificially loaded. Both beetle sex and position on the foliage never influence insect mortality or O. novo-ulmi infection. The best results were obtained injecting the Carbendazim?+?Acephate blend in the clone A. High chemical concentrations did not improve the general results.