Effects of non-chemical soil fumigant treatments on root colonisation with arbuscular mycorrhizal fungi and strawberry fruit production

The effects of biofumigation and soil heating on arbuscular mycorrhizal fungi (AMF) colonisation, strawberry growth and strawberry yield in pot experiments compared with untreated soil and chemical fumigation with dazomet were tested. Three different Brassicaceae species (Brassica juncea, Eruca sativa, Sinapis alba) were used as biofumigant plant green manure and soil heating was applied to simulate soil solarisation. Half of the plants were inoculated with indigenous arbuscular mycorrhizal fungi inoculum. With one exception (E. sativa) among the uninoculated plants, the treatments significantly decreased the mycorrhizal colonisation parameters compared with the untreated control. Dazomet displayed the greatest inhibitory effects on AMF establishment. In addition, the intensity and number of bands corresponding to Glomus spp. obtained with temporal temperature-gradient gel electrophoresis were lower for strawberry plants from biofumigant treatments than from the control. For the inoculated plants, there were almost no significant differences among the mycorrhizal colonisation parameters. The mass of leaves for the uninoculated and inoculated plants was higher for almost all non-chemical soil fumigant treatments compared with the control, except for heating of the uninoculated treatments. The number of strawberry fruits for the uninoculated biofumigant treatments was the highest, being higher than the values observed for the heating treatments, the chemical disinfection treatments and the control. There were no significant differences among the inoculated treatments. Biofumigation with Brassicaceae species resulted in higher soil organic matter and mineral nutrients and had a relatively small effect on AMF colonisation (F% = 59.0, 80.3, 47.3 for Bj, Es and Sa, respectively) compared with uninoculated controls (F% = 84.3). Despite the reduced AMF colonisation, biofumigation resulted in a higher fruit number and mass of leaves. Therefore, it represents a non-chemical soil fumigation method that should be applied in sustainable strawberry production.     

Growing Brassica juncea as a cover crop, then incorporating its residues provide complementary control of Rhizoctonia root rot of sugar beet

Biofumigation is increasingly viewed as a potentially useful technique for controlling soil-borne crop pathogens, but its efficacy has not systematically been demonstrated at field scale. We investigated the differences in efficacy observed in the field, by analysing the mechanisms by which a Brassica cover crop can act as a biofumigant crop in the prevention of soil-borne disease development. We hypothesised that the biofumigant crop might have a negative effect on soil-borne pathogens whilst growing, and that the pulverisation of this crop and the incorporation of its residues into the soil may enhance this effect. We tested this hypothesis by carrying out three field experiments in 2006, 2007 and 2008 in which Brassica juncea (brown mustard) was managed in different ways within a sugar beet–winter wheat rotation and analysing effects on sugar beet root rot caused by Rhizoctonia solani. Three treatments were studied: mustard pulled out at flowering (MP), mustard crushed at flowering and incorporated into the soil (MC) and bare soil (BS) as a control. We assessed the effect of each treatment on root rot incidence and severity at harvest. Over the 3 years of the experiment, disease incidence was significantly higher on BS plots than on the other plots and was significantly higher on MP plots than on MC plots. MC treatment gave a significantly lower mean conditional severity (severity calculated for diseased beets only) than the BS and MP treatments. Mustard residue incorporation was consistently effective at decreasing disease incidence from year to year (43, 44 and 47% efficacy, as determined by comparison with the disease incidence on BS plots, in 2006, 2007 and 2008, respectively), but the efficacy of growing mustard was variable (36, 16 and 39% efficacy in 2006, 2007 and 2008, respectively). These findings provide insight into the mechanisms by which biofumigant crops may affect soil-borne diseases. These findings have implications for the possible use of biofumigant crops as a biological method for controlling soil-borne diseases at the field scale.     

Evaluation of fungal epiphytes isolated from banana fruit surfaces for biocontrol of banana crown rot disease

Fungi isolated from the surface of banana fruits were evaluated for in vitro antagonism towards Lasiodiplodia theobromae. Thirteen fungi exhibiting pronounced growth inhibition of test pathogens were further tested for antibiosis against Thielaviopsis paradoxa, Colletotrichum musae, and Fusarium verticillioides. Clonostachys byssicola, Curvularia pallescens, Penicillium oxalicum, and Trichoderma harzianum were antagonistic to all test pathogens. Inhibition by C. pallescens and P. oxalicum to pathogens was at a distance, while C. byssicola and T. harzianum directly parasitized and killed the pathogens. The metabolites of C. byssicola, C. pallescens, and T. harzianum significantly affected the mycelial growth and conidial germination of the pathogens. In the artificial inoculation study, the antagonists survived and colonized banana fruits after 3 d. Interfungal parasitic relationship was observed between the antagonist and pathogen on artificial media and natural substrate. Postharvest application in the packing house showed that the incidence of crown rot in antagonist-treated banana was significantly lower when compared to fungicide and untreated control fruits.     

Activities of essential oils from Asarum heterotropoides var. mandshuricum against five phytopathogens

Some secondary metabolites of plants function as antimicrobial products against phytopathogens and constitute an increasingly important class of pesticides. In the present study, the essential oil of Asarum heterotropoides var. mandshuricum was analyzed by GC/MS and its antimicrobial activity was evaluated against five phytopathogenic fungi. Major components of the oil were methyleugenol (59.42%), eucarvone (24.10%), 5-allyl-1,2,3-trimethoxybenzene (5.72%), and 3,7,7-trimethylbicyclo(4.1.0)hept-3-ene (4.93%). The essential oil and the most abundant component, methyleugenol, were separately assayed for inhibition of 5 pathogens: Alternaria humicola, Colletotrichum gloeosporioides, Rhizoctonia solani, Phytophthora cactorum and Fusarium solani. Both the oil and methyleugenol strongly inhibited the growth of the test pathogens (IC₅₀ values <0.42 μg ml⁻¹) except F. solani, with the best activity against P. cactorum (IC₅₀ values = 0.073 and 0.052 μg ml⁻¹, respectively). It is concluded that the essential oil of A. heterotropoides var. mandshuricum has a broad antiphytopathogenic spectrum, and that methyleugenol is largely responsible for the bioactivity of the oil. The mode of action of methyleugenol against P. cactorum is discussed based on changes in the mycelial ultrastructure.     

Screening of Natural Plant Volatiles to Control the Potato ( Solanum tuberosum ) Pathogens Helminthosporium solani , Fusarium solani , Phoma foveata and Rhizoctonia solani

The potential utility of natural volatiles in various essential oils (EOs) from plants as fumigants to control potato tuber (Solanum tuberosum L.) pathogens was assessed. The antifungal effects of the volatiles at various concentrations were studied at 10 °C both in vitro using conidial suspensions of Helminthosporium solani, Fusarium solani var. coeruleum and Phoma foveata plated on agar, and in vivo by inoculating potato tubers. The effects of the volatiles on the mycelial growth of Rhizoctonia solani were also studied, but only in vitro. Vapours of many of the EOs tested exhibited some fungicidal activity but volatiles of garlic, Allium sativum, were, with few exceptions, most effective on all four pathogens in all experiments. An exposure time of at least 2 weeks was usually required for good control of disease development in vivo. Vapours of A. sativum never stimulated conidial germination, as was observed with some other oils, or damaged tubers, but those of Armoracia rusticana caused tuber collapse. Volatiles from thyme (Thymus vulgaris) EO showed antifungal activity in vitro on all four pathogens, but did not control F. solani, P. foveata or H. solani in vivo. In contrast, sage (Salvia officinalis) EO was ineffective against P. foveata and H. solani in the in vitro system, but controlled disease development in vivo at similar doses. The sometimes conflicting results obtained in the two test systems show that screening in vitro only is insufficient for evaluation of potent antifungal substances to be used in practice.     

Causes of differences in growth pattern,yield and quality of potatoes (Solanum tuberosum L.) in short rotations on sandy soil as affected by crop rotation,cultivar and application of granular nematicides

Summary A crop rotation experiment was carried out on a light sandy soil in 1979–1986 to study the effects of the frequency of potato cropping on yield, quality and on the occurrence of soil-borne pathogens other than potato cyst nematodes. Tuber yield decreased markedly with increasing cropping frequency, but also depended on what crops were grown in rotation with potato. Growth during the early part of the season, as well as the length of the growing period, were reduced in short rotations. The fungiVerticillium dahliae andRhizoctonia solani, and root-knot nematodes (Meloidogyne spp.) were the most important yield reducing pathogens. The effects of rotation depended on the cultivar used. The percentage of mis-shapen tubers increased with increasing cropping frequency and after application of granular nematicides, but the incidence of common scab (Streptomyces scabies) was not affected.     

河南省3种麦田阔叶杂草对苯磺隆的抗性

为明确河南省冬麦田3种阔叶杂草猪殃殃、播娘蒿、牛繁缕对苯磺隆的抗性水平和分布现状,在温室中采用整株剂量-反应测定法测定了3种杂草的40个种群对苯磺隆的敏感性。结果表明,河南省3种阔叶杂草已普遍对苯磺隆产生抗性,所测样本中,抗性种群27个,占总采样数的67.50%,其中牛繁缕抗性水平最高,猪殃殃居中,播娘蒿最低。豫中和豫南地区3种杂草的整体抗性水平较高,豫西和豫西南抗性水平相对较低。     

<Emphasis Type="BoldItalic">In Vitro</Emphasis> Antioxidant and Anti-rhizopus Activities of Lamiaceae Herbal Extracts

Eighty-eight extracts of different polarity obtained from 18 Lamiaceae medicinal and aromatic plants were screened for their antioxidant and antifungal properties. A TLC analysis combined with two bioassays were used to detect these activities. In most cases the highest radical scavenger capacity was detected in methanolic and aqueous extracts and polyphenols may be responsible. The most interesting antioxidant activity was observed in polar extracts obtained from Lycopus europaeus, Melissa officinalis, Origanum vulgare subsp. virens and Lavandula latifolia. On the contrary, the best results for the antifungal test against Rhizopus stolonifer were produced by non-polar herbal extracts. Phlomis lychnitis, Salvia pratensis and Calamintha sylvatica caused the highest inhibition on Rhizopus growth.     

Effects of soil solarization on soil-borne populations ofPythium aphanidermatum andFusarium solani and on the potato crop in Tunisia

Summary Soil solarization by mulching with polyethylene for 60 days during summer in Tunisia, was effective in reducing dramatically to a depth of 30 cm inoculum levels ofFusarium solani (Fs) from 3,000 to 450 propagules gram⁻¹ of dry soil. Population densities ofPythium aphanidermatum (Pa) were reduced from 1,700 and 3,000 to 0.5 and 1 propagule gram⁻¹ of dry soil in the top 15 cm and at 1–30 cm depth, respectively. After a subsequent autumn potato crop, populations ofPa remained very low in the previously solarized soil whereas propagule density in non-solarized soils was similar to the initial density. Also, inoculum level ofFs increased by 40% in nonsolarized soils while it remained unchanged in solarized soils, suggesting microbial processes that prevented soil reinfestation. All the annual weeds were eradicated by solar heating but not the perennial weedConvolvulus arvensis. Potato plant growth and yields were improved in solarized plots.     

Growth Stage of Cyperus esculentus Influences Its Allelopathic Effect on Ectomycorrhizal and Higher Plant Species

Summary

Live plants and plant material in various forms have important roles in ecological processes. Weeds may be involved in interactions with desirable species at different trophic levels through the production and release of allelochemicals. The allelopathic potential of the weed Cyperus esculentus (yellow nutsedge) was assessed by preparing aqueous extracts from tubers and foliage from immature or mature plants, and testing their effects on an ectomycorrhiza and two higher plant species. The extracts from tubers and both foliage types significantly reduced the colony diameter of the ectomycorrhiza Boletus maxaria, which was isolated from roots of the forestry species Pinus patula, on agar medium. Extracts (2% m/v) from C. esculentus tubers and the foliage of immature plants inhibited the germination of lettuce (Lactuca sativa) seeds significantly. At 5% concentration, the extracts from foliage of immature weed plants completely inhibited the germination of lettuce seeds at the end of the 7-day trial period. The emergence of corn (Zea mays) was retarded in soil where C. esculentus tubers were planted 28 days before planting the crop, irrespective of whether the weeds continued to grow or were physically removed at the time the corn was sown. In contrast, where weed tubers and corn seeds were planted at the same time, emergence of corn was not affected. It is suggested that the growth stage of weeds be considered in the assessment of their direct and indirect allelopathic effects, and in predictions for their impacts on crop development and yield.     

Yield losses of soybean and maize by competition with interseeded cover crops and weeds in organic-based cropping systems

Weed management is a major issue in organic farming systems. Although interseeding cover crops is one alternative to herbicides, cover crops often suppress not only weeds but also main crops. Therefore, using cover crops for weed control without adverse effects on main crop growth is important. To verify the effect of cover crops on competition between main crops, cover crops and weeds in a snowy-cold region, main crops soybean (Glycine max Merr.) in 2005 and maize (Zea mays L.) in 2006 were grown with cover crops winter rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth), respectively. The cover crops were sown on three sowing dates: before main crop planting (Pre-MC), on the same date of main crop planting (Syn-MC) and after main crop planting (Post-MC). A plot without cover crops (Sole-MC) was used as a control. The dry weight (DW), vegetation cover ratio (VCR), vertical community structure and chlorophyll content were measured to estimate the competition between main crops, cover crops and weeds. Weed DW was suppressed significantly by sowing cover crops in both soybean and maize. This weed suppression was associated with the increase of VCR of main crops plus cover crops at the early growth stage of main crops. Soil seed banks of dominant weed also became lower by sowing cover crops, implying the importance of proper weed management for suppressing weeds successively. In addition, the sowing dates of cover crops had large effects on main crops DW especially in maize, i.e., it was significantly lower in Pre-MC and Syn-MC than in Post-MC. Although the cover crop height was markedly shorter than the main crop height, the chlorophyll content of the main crops was significantly lower when cover crops were sown earlier. These results suggest that the growth inhibition of main crops by cover crops was partly caused by competition for nutrients between main crops and cover crops, and this growth inhibition was alleviated when cover crops were sown after the establishment of main crops. Consequently, soybean yield was the highest in Post-MC and decreased by 29%, 18% and 7% in Sole-MC, Pre-MC and Syn-MC, respectively, and maize yield was also the highest in Post-MC and decreased by 68%, 100% and 24% in Sole-MC, Pre-MC and Syn-MC, respectively. It was concluded that weeds could be controlled effectively by sowing cover crops after planting main crops in organic farming systems in a snowy-cold region.     

Weed Allelopathy,Its Ecological Impacts and Future Prospects

Summary

The importance, characteristics, positive and negative impacts, and future role of weeds as an integral part of the natural and agroecosystems are evaluated and discussed. Interference between plants in nature and the importance of differentiating between competition and allelopathy are interpreted. Allelopathy as one component of weed/crop interference, allelochemicals from weed species and their possible mechanism of action are listed and discussed. Weed species with inhibitory action against cultivated crops, other weed species, and plant pathogens, as well as self-inhibitory (autopathic) species are reviewed. Stimulatory or inhibitory allelopathic effects of different crop plants, trapping and catching species, and the potential of allelopathic weeds in inhibiting or stimulating certain parasitic weed species are discussed and evaluated. Allelopathy as a mechanism and future strategy for agricultural pest control and farm management and the potential use and development of some allelochemicals as natural pesticides or plant growth regulators are also considered and discussed.     

Effects of Application of Alfalfa Pellet on Germination and Growth of Weeds

Summary

Allelopathy is well known as an interaction among plants (including microbes) through chemicals produced by plants. Recently, many investigations have evaluated the possibility of the use of allelo-pathic substances as a natural herbicide. Alfalfa (Medicago sativa L.) is reported to contain allelopathic chemicals that inhibit the growth of weeds. Experiments were conducted in Laboratory of Crop Science, Experimental Farm of Miyazaki University and Kobayashi city in Miyazaki Prefecture, Japan to determine the allelopathic impact of alfalfa pellet (commercial forage fodder) on germination and growth of lowland weeds, and its potential as a natural herbicide in paddy field. The results indicated that alfalfa pellet significantly inhibited germination and growth of 4 weed species, viz., Echinochloa orygicola, Digitaria ciliaris, Cyperus difformis and Monocholia vaginalis in rice paddies. Among the 4 tested weeds, the maximum inhibitory effect of alfalfa pellet was seen against Cyperus difformis. The degree of inhibition of weed growth by alfalfa pellet became stronger as the application of concentration increased. The results showed that alfalfa pellet might be used as a natural herbicide in rice paddies and might reduce the amount of herbicide used in paddy fields.     

Crop Allelopathy and Its Role in Ecological Agriculture

Summary

A number of crops exhibit allelopathic interactions that play a significant role in the complex environment of agroecosystems. Several studies have shown that allelopathic crops reduce growth, development and yield of other crops growing simultaneously or subsequently in the fields. Another aspect of interest regarding crop allelopathy is that allelochemicals may exhibit inhibitory effect on the same crop which is commonly called as crop autotoxicity. It is predominantly common in fields where sole cropping under reduced or no-tillage system is practiced. Though any crop part can be allelopathic, including even the pollens, but decomposing crop residues exhibit more influence on other plants. Furthermore, the extent of allelopathy by a crop plant varies with age, part and type of cultivar being used. Nowadays allelo-pathic crops are being used as an important tool in managing weeds and harmful pests under sustainable pest management programs. In this context several cover/smother and green manure crops with allelopathic nature hold a good promise as well as challenge for the future as they have a potential to suppress noxious weeds. Likewise, the decomposing residues of such crops can also be used for managing harmful weeds and pests vis-à-vis maintaining the sustainability of the system. The traditional practice of crop rotation which declined with the discovery of synthetic herbicides can also be revived, if allelopathic potential of rotational crops is well understood. Selection of cultivars with high allelopathic potential/high competitiveness is another possible way by which crop allelopathy can be better utilized for weed control and yield enhancement. Crops with less allelopathic potential can be genetically improved by incorporating desired genes encoding the synthesis of allelochemicals. Pure allelochemicals extracted and identified from some crop plants can also be used as bioherbicides.     

盛花期油菜还田对土壤微生物和后茬花生的影响

为明确油菜作为绿肥对土壤微生物和后茬花生生长的影响,选用种子硫苷含量不同的甘蓝型油菜和芥菜型油菜盛花期地上部作为绿肥,以盆栽方式模拟绿肥归田。结果表明：相比低硫苷甘蓝型油菜,高硫苷的芥菜型油菜不同生育期植株硫苷含量相对较高,花期植株硫苷含量差异显著。模拟还田后30d内,两类油菜植株体内硫苷的降解产物异硫氰酸酯（ITC）,均在20d时出现最高峰值。芥菜型油菜硫苷含量高,作为绿肥用其抑制细菌和真菌 的能力较强,而低硫苷甘蓝型油菜肥用,对真菌无抑菌能力。芥菜型油菜还田能促进后茬花生生长,花生有效根瘤数和单株干重分别比对照增加11.4%和7.1%,均达到了显著水平。     

Neonectria liriodendri sp. nov., the main causal agent of black foot disease of grapevines

Black foot disease is a serious disease of grapevine crops in most areas where vines are grown. Mainly two species of Cylindrocarpon, C. destructans and C. macrodidymum, are associated with this disease. Recent studies have revealed a tremendous molecular variation within the former but only slight molecular variation within the latter, indicating that C. destructans presents a complex of several species The present study elucidates the taxonomic status of C. destructans-like isolates associated with black foot disease of grapevines. Grapevine isolates were studied morphologically, subjected to DNA analyses of their ITS and partialβ -tubulin genes, and were mated in all combinations in vitro. Cylindrocarpon destructans strains isolated from grapevines in Europe and South Africa appeared morphologically and genetically identical, and had identical ITS and partial β-tubulin gene sequences. Phylogenetic analyses placed these strains in a clade closely related but clearly distinct from other clades with C. destructans-like anamorphs obtained from various herbaceous or woody hosts. Only the ex-type strain of Cylindrocarpon liriodendri had identical sequences to strains isolated from grapevines, and could also not be distinguished by morphological characters. The grapevine isolates are therefore reidentified here as Cylindrocarpon liriodendri. Cylindrocarpn liriodendri formed perithecia in heterothallic conditions and the holomorph of this species is described as Neonectria liriodendri sp. nov. Neonectria liriodendri is genetically distinct from the ex-type strain of Neonectria radicicola, which originated from Cyclamen in Sweden. Both ex-type strains also differ from at least two other clades comprising additional C. destructans-like strains. Many of these strains originated from Panax sp., which is the host of the type of C. destructans. Our phylogenetic analyses indicate that C. destructans is not the anamorph of N. radicicola and that N. liriodendri, N. radicicola and several C. destructans-like taxa may have evolved independently within the same phylogenetic species complex.Taxonomic novelty: Neonectria liriodendri Halleen, Rego& Crous sp. nov.     

Inhibitory effects of cover crop mulch on germination and growth of Stellaria media (L.) Vill., Chenopodium album L. and Matricaria chamomilla L.

Cover crops may suppress weeds due to their competitive effects and the release of inhibitory compounds. We examined the inhibitory influence of 11 cover crop mulches on the germination and growth of weed species (Stellaria media (L.) Vill., Chenopodium album L. and Matricaria chamomilla L.) in laboratory, greenhouse and field experiments. In the laboratory, cover crop extracts were tested in germination bioassays at six concentrations (0–500 mg ml⁻¹). The germination rate and root length (i) were measured 10 days after treatment (DAT). Pot experiments were carried out in the greenhouse to investigate the effects of cover crop mulch (ii) incorporated into the soil on weed germination and weed dry mass. Field trials measured the suppressive effects of cover crops and cover crop mixtures on weeds (iii). Correlations were determined between the experiments to quantify the competition and the biochemical effects of cover crops separately. Cover crop extracts at a concentration of 125 mg ml⁻¹ (i) significantly reduced the weed germination rate by 47% and the root length by 32% on average. M. chamomilla showed a lower susceptibility to the extracts of S. alba, R. sativus var. niger and H. annuus compared to C. album and S. media. The mulch-soil mixtures (ii) significantly reduced the germination rate by 50% and the dry mass by 47% on average across all three weed species, while M. chamomilla showed the highest tolerance to the mulches of V. sativa and A. strigosa. The correlation analysis revealed a strong positive correlation between extract toxicity and field weed suppression and, thus, indicated a high impact of the biochemical effects of the tested cover crops on weed suppression, especially for S. media and M. chamomilla.     

Annual forage legumes in dryland agricultural systems of the West Asia and North Africa Regions: research achievements and future perspective

Forage legumes are vitally important to animal production in the dryland farming systems of the Mediterranean region. Of the diverse forage‐legume species adapted to the Mediterranean climate, vetches, (Vicia spp.), chicklings (Lathyrus spp.), annual medics (Medicago spp), clovers (Trifolium spp.) and species of the Lupinus, Lotus, Onobrychis, Hedysarum and Ornithopus genera are considered to be the most agronomically important and economically valuable species for the region. Adoption of perennial self‐regenerating medic (Medicago spp.) has been limited because of technical difficulties, but annual vetch (Vicia spp.) has the greatest potential as a viable animal‐feed source and a rotation crop with cereals. Some forage legumes survive harsh conditions by their unique underground growth habit, for example, V. amphicarpa and Lathyrus ciliolatus. Efforts to improve forage legumes have been based on both management/cultural factors and breeding. Research based on several long‐term barley‐ and wheat‐based rotation trials has demonstrated the viability of forage legumes, especially vetch, in the region's improved farming system. An additional benefit to such legumes is the enhancement of soil quality, that is, soil fertility, soil organic matter and soil physical properties. Thus, the development of forage legumes is essential to agricultural sustainability in the Mediterranean region and in other dryland cereal‐growing areas of the world where grazing livestock is a dominant enterprise. To build upon the considerable research conducted on forages, intensified efforts are needed to develop locally adapted forage cultivars, to provide economic assessment of forages in cropping systems and to promote technology transfer at the farm and community level.     

砜吡草唑除草活性及对玉米的安全性评价

砜吡草唑是一种新型、高效、广谱的土壤处理除草剂,为探究其对玉米田常见杂草的除草活性及对玉米的安全性,通过室内生物测定和田间药效试验测定砜吡草唑的杀草谱、除草活性及其对玉米的安全性。室内生物测定结果表明,砜吡草唑在90 g(a.i.)/hm2时,对牛筋草、马唐、狗尾草、稗草、碎米莎草、青葙、铁苋菜等多种常见秋熟作物田杂草均具有较好活性,鲜重抑制率均大于90%;对4种常见玉米田杂草马唐、稗草、青葙、碎米莎草的GR50分别为14.77、16.41、15.37、27.74 g(a.i.)/hm2,除草活性在供试剂量下均高于对照药剂精异丙甲草胺。砜吡草唑在供试的3个玉米品种奥玉510、郑单958、蠡玉16和4种杂草马唐、稗草、青葙和碎米莎草之间具有较好的选择性,选择性指数为23.82～240.88。田间药效试验表明,砜吡草唑在250 g(a.i.)/hm2时,对马唐、稗草、铁苋菜、碎米莎草的总鲜重防效达91.4%,对供试玉米蠡玉16安全。     

Field assessment of the relative importance of different aphid species in the transmission of potato virus Y

Summary Flying aphids were trapped throughout the summer on a vertical net downwind of a plot of PVY-infected potato plants. Of 6769 individuals caught, 165 transmitted PVY to tobacco test seedlings. Of 119 species or species groups caught, 20 were found to be vectors of which nine had not been recorded previously.Brachycaudus helichrysi, Myzus persicae, Phorodon humuli andAphis species accounted for 90% of transmissions andB. helichrysi alone for 52% of transmissions. The prospects of using this information to assess the amount of virus spread in a potato crop and of forecasting the timing and abundance of the main vectors are discussed.

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Zusammenfassung Im Abwind einer Parzelle mit PVY-infizierten Kartoffelpflanzen (7% PVY^O, 4% PVY^N, 89% beide) wurden fliegende Blattl?use in zwei vertikalen Netzen (4,4 m × 1,4 m mit hexagonalen L?chern mit 1,5 mm Durchmesser) gefangen und anschliessend auf Tabaks?mlinge überführt, um zu prüfen, ob sie PVY übertragen. Die Blattl?use wurden vom 8. Juni bis zum 8. August 1984 normalerweise an einem Tag pro Woche von 10 bis 17 Uhr gefangen. Im Laufe von 12 Fangtagen wurden 6769 sachte 52% der übertragungen bei nur 15% der totalen Fangzahl (Tab. 3).B. helichrysi mitMyzus persicae, Phorodon humuli und Species des GenusAphis ergaben sich als 90% der übertragenden Individuen bei nur 32% der gefangenen Proben. Acht Prozent der gefangenenB. helichrysi waren übertr?ger, dagegen nur 4,7% vonM. persicae. B. helichrysi k?nnte als Vektor besonders wichtig sein, weil sie in der Vegetationszeit Blattl?use von 119 Species oder Speciesgruppen gefangen, von denen 165 (2,44%) Tabak mit PVY inokulierten (Tab. 1). 133 Blattl?use übertrugen PVY^O und 32 übertrugen PVY^N (und m?glicherweise auch PVY^O). 20 Species oder Speciesgruppen waren Vektoren, von denen 9 (Cryptomyzus ballotae, Hyperomyzus lactucae, Metopolophium festucae, Myzus ligustri, M. myosotidis, Myzaphis rosarum, Sitobion avenae, S. fragariae undUroleucon spp.) erstmalig beschriebene Vektoren sind.Brachycaudus helichrysi verurfrüh auftritt (Tab. 2), wenn die Pflanzen am anf?lligsten sind und die Virus-Translokation zu den Knollen am schnellsten stattfindet. Die Aussichten zum Gebrauch dieser Information zur Absch?tzung des Aufbaues von Virusbefall im Bestand und die Aussichten für eine Vorhersage der Menge der wichtigsten Vektoren werden diskutiert. Es ist dringend erforderlich, die Biologie vonB. helichrysi zu untersuchen.

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Résumé Deux filets verticaux (4,40 m × 1,40 m à mailles héxagonales de 1,5 mm de diamètre) sont placés face au vent pour la capture des pucerons dans une parcelle de plants de pommes de terre contaminés par PVY (7% PVY^O, 4% PVY^N, 89% par les deux) puis les pucerons sont transferrés sur des plantules de tabac en milieu clos pour tester la transmission de PVY. Les captures de pucerons ont eu lieu du 8 juin au 8 ao?t 1984, en général à raison d'un journée par semaine, de 10 heures à 17 heures. Pendant les 12 premiers jours, 6769 pucerons de 119 espèces ou groupes d'espèces ont été capturés, les plantules de tabac ayant été inoculées par PVY avec 165 pucerons (2,44%) (tabl. 1). 133 pucerons étaient vecteurs de PVY^O et 32 de PVY^N (et probablement PVY^O). 20 espèces ou groupes d'espèces étaient des vecteurs dont 9 récemment enregistrés (Cryptomyzus ballotae, Hyperomyzus lactucae, Metopolophium festucae, Myzus ligustri, M. myosotidis, Myzaphis rosarum, Sitobion avenae, S. fragariae etUroleucon spp.).Brachycaudus helichrysi représentait 52% des transmissions mais seulement 15% du total capturé (tabl. 3).B. helichrysi, Myzus persicae, Phorodon humuli et les espèces du gèneAphis représentaient 90% des transmissions et 32% des captures seulement. 8,0% deB. helichrysi capturé a transmis le virus contre, 4,7% pourM. persicae. B. helichrysi peut être particulièrement important comme vecteur dans la mesure ou il appara?t t?t en saison (tabl. 2) lorsque les plantes sont très sensibles et la translocation di virus aux tubercules la plus rapide. Les perspectives à partir de cette connaissance sont discutées en vue d'analyser l'évolution du virus dans une culture et les prévisions de l'abondance des plus importants vecteurs. Une plus grande connaissance de la biologie deB. helichrysi devient urgente.