不同镉处理对两种生态型水蜈蚣镉富集特性的影响

采用室内盆栽试验,研究了在不同浓度cd处理下,两种生态型水蜈蚣的生长和对cd的吸收和富集特性。结果表明：（1）在2mg·kg^-1Cd处理时,Cd对两种生态型水蜈蚣生物量的影响较小。之后随着cd处理浓度的增加,两种生态型水蜈蚣生物量均呈降低的趋势,且差异显著。在200mg·kg^-1Cd处理时,矿山生态型水蜈蚣死亡,而非矿山生态型仍能维持一定生长,表现出较强的cd耐性。（2）在高浓度cd处理下,非矿山生态型水蜈蚣比矿山生态型具有更强的Cd富集能力。在2、10、50mg·kg^-1Cd处理时,两种生态型水蜈蚣Cd含量和积累量均呈增加的趋势。在200mg·kg^-1Cd处理时,非矿山生态型水蜈蚣地上部和地下部Cd含量分别达到498．66mg·kg^-1和1016．09mg·kg^-1,积累量分别为86．00μg·pot^-1和123．82μg·pot^-1,具有较强的cd富集能力。（3）在不同浓度Cd处理下,两种生态型水蜈蚣地上部富集系数均大于1,表现出较强的cd富集能力;矿山生态型水蜈蚣转移系数最大值为0．55,非矿山生态型为0．53。两种生态型水蜈蚣对cd均有一定的富集特性,而在高浓度Cd处理时非矿山生态型水蜈蚣的cd富集能力更强。     

氮肥形态和地膜覆盖对坡耕地玉米产量和土壤氮素流失的影响

采用二因素四水平随机区组试验设计,利用模拟径流小区观测的方法,研究氮肥形态和覆盖对坡耕地玉米产量及土壤氮素流失通量及途径的影响。结果表明:土壤径流以地表径流为主,氮素主要流失途径是地下径流,占总径流量的71.3%,氮素流失的主要形态是硝态氮,占无机氮的92.0%,占水溶性总氮的57.1%。地膜覆盖降低径流深和土壤侵蚀量,增加水溶性氮流失量,提高玉米产量,但是酰胺态氮肥和缓控释肥地膜覆盖可降低氮素流失量,且酰胺态氮肥对玉米增产效果显著;裸地条件下硝态氮肥和缓控释肥可降低地下径流深和氮素流失量,且缓控释肥对玉米增产效果显著。综合考虑水土流失治理和粮食增产增收,四川紫色坡耕地适宜肥料类型为缓控释肥,其次是硝态氮肥,酰胺态氮肥通过地膜覆盖也能较好地控制氮素流失和增加玉米产量。     

不同形态氮肥对磷肥生物有效性的影响

利用盆栽试验研究了潮土中不同形态氮肥对磷肥生物有效性的影响。结果表明:不同形态氮肥处理均有抑制油菜生长初期磷素累积的作用,氯化铵处理抑制作用最突出,其次是尿素,再次是硫酸铵、硝酸铵和硝酸钙处理;油菜生长后期不同形态氮肥处理均逐渐显著促进油菜的磷累积,但氯化铵处理的促进作用仍然低于其它氮肥处理,这与不同形态氮肥处理对油菜生长的影响较一致。土壤有效磷含量受不同形态氮肥处理影响较小,而水溶性磷含量因施用不同形态氮肥均表现显著地下降,氯化铵和硫酸铵处理使土壤水溶性磷下降较多,硝酸铵、硝酸钙和尿素处理使土壤水溶性磷下降较少。     

不同形态氮肥及其运筹对春玉米产量和农艺性状的影响

通过3年田间定位试验,研究不同氮肥管理方式对春玉米产量、地上部吸氮量和收获指数的影响。结果显示,连续3年不同形态氮肥(尿素、硫铵和硝钙)、氮肥运筹(全部底肥即"一炮轰"和基追比1∶2)、传统施氮量(190kg.hm-2)、减氮20%(150kg.hm-2)处理条件下,春玉米籽粒产量(10612～11906kg.hm-2)、收获指数(0.46～0.50)和地上部吸氮量(192～214kg.hm-2)没有显著差异;施肥与不施肥、不施氮肥处理相比,产量增加显著。通过农艺性状对玉米产量的通径分析表明,穗粒数和行粒数通过直接和间接作用对产量起重要影响,而不同氮肥处理对百粒重影响不明显,百粒重对产量的直接和间接作用也没有达到显著水平。玉米收获后,除不施肥处理外,不同氮肥处理间0～100cm土壤剖面的硝态氮含量没有显著差异。说明在正常年份,施氮150kg.hm-2与磷钾配施,可以达到吉林春玉米高产的目的。     

氮肥不同施用时期对春玉米早衰的影响

采用盆栽试验研究氮肥不同施用时期下春玉米早衰特性。结果表明,合理的增加追肥次数能提高穗粒数和千粒重,进而提高玉米的籽粒产量。当氮肥分别在基施、拔节期与大喇叭口期1∶3∶1施用时,春玉米生育后期的穗叶叶绿素含量和SOD活性较一次性基施有不同程度的提高,而叶片形态指标和丙二醛(MDA)含量却降低,有效地延缓了玉米的衰老。对照(无氮肥)处理的穗叶叶绿体破裂解体,基粒和基质片层结构膨胀,导致叶片的叶绿素含量下降、光合能力降低而出现早衰。     

不同缓控释氮肥对连作春玉米产量及氮肥去向的影响

在山西省连作春玉米区连续4年设置大田定位试验,设置不施氮肥(CK)、一次性基施尿素(CU1)、追施尿素(CU2)、树脂包膜尿素(PCU)、硫包衣尿素(SCU)、多酶金缓释尿素(MEU)6个施肥处理,研究施用缓控释氮肥对春玉米产量、氮肥去向及氮素平衡的影响,为春玉米氮素养分的科学管理技术提供参考。结果表明:(1)缓控释氮肥处理能够明显提高春玉米产量,促进氮素吸收。与CU1处理相比,SCU、MEU、PCU和CU2处理可分别提高春玉米产量17.51%,9.88%,9.62%,9.48%,同时氮肥农学利用效率分别提高7.5,4.2,4.1,4.1 kg/kg。(2)不同缓控释氮肥处理的作物吸收肥料氮以及肥料氮在0-100 cm土层残留量之间存在显著差异。SCU、MEU、PCU、CU2和CU1的氮肥表观利用率分别为36.1%,32.5%,26.5%,26.7%,19.5%,肥料氮在0-100 cm土层残留量分别占施氮量的28.5%,31.6%,35.7%,35.5%,39.1%。此外,与一次性基施尿素相比,缓控释氮肥能够显著降低肥料氮的损失,SCU、MEU、PCU和CU2分别降低了22.65%,18.81%,8.99%,8.47%。(3)综合分析不同氮肥处理的农田氮素平衡,SCU处理的春玉米吸氮量最高,为261.5 kg/hm^2,其次是MEU,为253.5 kg/hm^2。SCU的0-100 cm土层残留量在缓控释氮肥中最低,为124.1 kg/hm^2,MEU和PCU分别为131.04,140.09 kg/hm^2。SCU处理的氮表观损失量最低,为106.3 kg/hm^2,MEU和PCU分别为111.6,125.1 kg/hm^2。在山西省春玉米主产区土壤上,缓控释氮肥能够显著促进春玉米对氮素的吸收,减少氮素损失。硫包衣尿素和多酶金缓释尿素的效果相对较好。     

不同氮肥管理对吉林春玉米生长发育和养分吸收的影响

针对吉林春玉米氮肥施用中存在氮肥用量偏大,且具盲目性的现状,采用田间试验研究农民习惯施氮量和推荐施氮量下氮肥不同施用方式对春玉米干物质积累、子粒产量、氮素吸收和利用效率的影响。结果表明,推荐施氮量下,苗期和灌浆期春玉米干物质积累量显著高于习惯施肥,有机无机配合提高了春玉米干物质积累速率。施氮处理与不施氮相比均显著增加玉米子粒产量,增产11.2%～16.8%; 推荐施氮量下玉米子粒产量与习惯施氮量相当,但显著提高氮素的偏因子生产力和农学效率。在氮磷钾用量一致的基础上,用30%有机肥氮替代化肥氮与100%化肥氮处理的产量相当,对氮素利用效率也没有影响,并降低收获期土壤无机氮含量。说明合理施用氮肥不但能够维持玉米产量,还可减少氮肥投入,提高氮肥利用效率;有机肥部分替代化肥氮是吉林春玉米氮素管理的有效途径之一。     

稳定氮肥用量对夏玉米产量和氮肥利用率的影响

通过大田小区试验,研究了稳定氮肥不同用量对夏玉米产量、养分累积量、氮肥利用效率及经济效益的影响。结果表明,与不施稳定氮肥相比,稳定氮肥施氮量90、150、210 kg·hm-2和270 kg·hm-2分别增产36.7%、62.1%、76.6%和81.9%,地上部氮素总累积量分别增加39.0%、60.3%、79.0%和113.4%,经济效益分别增加36.1%、61.2%、72.7%和77.1%;与农民习惯施用氮肥相比,高量稳定氮肥用量210 kg·hm-2和270 kg·hm-2分别增产7.3%和10.5%,地上部氮素总累积量分别增加3.2%和23.0%,经济效益分别增加9.7%和11.4%。施用稳定氮肥促进夏玉米对氮素的吸收累积,高量210 kg·hm-2和270kg·hm-2处理较习惯施氮提高总吸氮量。施用稳定氮肥各处理氮肥表观利用率和农学效率显著高于农民习惯施氮,偏生产力高于农民习惯施氮,生理效率除270 kg·hm-2处理外,高于农民习惯施氮。稳定氮肥施氮量在210 kg·hm-2时,能较好地协调玉米高产与稳定氮肥合理利用的统一。     

红壤长期不同施肥对玉米氮肥回收率的影响

为阐明长期不同施肥下玉米氮肥回收率的变化特征,揭示影响红壤玉米氮肥回收率的关键土壤因素,为红壤氮肥合理施用和提高氮肥回收率提供科学依据,在湖南红壤上连续观测了18年不施肥,施氮、磷、钾化肥,化肥配施有机肥和单施有机肥条件下玉米氮肥回收率的变化特征及其原因。结果表明,与施用化肥相比,玉米的子粒产量和植株氮素积累量在化肥配施有机肥下分别增加了61.5%和63.1%。氮、磷、钾化肥施用8年后,玉米氮肥回收率呈显著下降趋势,年平均下降速率达3.34个百分点;而化肥配施有机肥下氮肥回收率以每年1.24个百分点的速率显著增加;单施有机肥下氮肥回收率保持持平并略有上升。土壤pH、全氮和有机质含量均与氮肥回收率呈显著线性正相关,其中土壤pH对氮肥回收率的影响最明显。在土壤pH 4.5～6.3范围内,红壤pH每降低1个单位,玉米氮肥回收率下降10.9%。长期施用化肥的土壤pH以每年0.06的速率下降;单施有机肥尽管可缓解土壤酸化,但玉米产量较低,而化肥配施有机肥能同时抑制土壤酸化和提高玉米产量及氮肥回收率。因此,化肥配施有机肥具有明显的增产和保肥效果,是红壤上农业生产可持续性的施肥模式。     

秸秆还田和氮肥施用对夏玉米氮肥利用率的影响

本文研究了秸秆还田和N肥施用对夏玉米N肥利用率的影响。结果表明,秸秆还田和N肥施用都有助于夏玉米N肥利用率的提高,尤其是优化施N大大提高了夏玉米的N素利用率,并且提出了在京郊地区适宜推广的一种可行的培肥地力、增加产量的模式。     

氮肥类型对夏玉米氮素吸收和利用的影响

连续两年在吴桥实验站进行田间试验,研究了3种类型氮肥(尿素、包膜尿素和复合肥)对夏玉米郑单958(紧凑型)和农大108(半紧凑型)氮素吸收与利用效率的影响。结果表明,随施氮量增大,夏玉米氮累积量(NAA)显著增加,氮素吸收效率(NUPE)、氮生理效率(NPE)、氮素利用效率(NUTE)、氮收获指数(NHI)降低,氮肥利用率(NUE)、氮肥效率(NFE)显著降低。氮肥类型影响夏玉米氮素吸收与利用效率,NUE、NUPE以包膜尿素和复合肥处理较大,而NPE和NUTE以尿素处理较高,但差异不达显著水平;NHI一般以尿素处理较高,其差异显著性存在品种间、年际间及施氮水平间差异。夏玉米NAA、NHI、NFE等具有较明显的基因型差异,均表现为郑单958大于农大108;两品种在氮素利用上均属敏感型,但郑单958的敏感性强于农大108。可见,施氮量是影响夏玉米氮高效利用的关键因素,而合理选择品种与氮肥类型也能起到一定的积极作用。本试验条件下,选择紧凑型品种与复合肥对提高华北平原夏玉米氮利用效率较为有利。     

Arsenic accumulation and distribution in the tissues of inbred lines in maize (Zea mays L.)

We investigated arsenic (As) accumulation and distribution in four different tissues of maize, leaves, stems, bracts, and kernels. For these analyses, we used 122 elite inbred lines, which were grown in an As-contaminated area in China. The agricultural soil at the study site is highly As-contaminated (11.02?±?0.95?mg?As?kg?soil^?1) because of excessive use of As-rich surface water for irrigation. The As concentration in the leaves was significantly positively correlated with that in stems and kernels, and the As concentration in the stems was significantly positively correlated with that in the kernels. In the 122 inbred lines, the highest average As concentration was in the leaves, followed by the stems, the bracts, and then the kernels. The range of As concentrations in leaves, stems, bracts, and kernels of the 122 inbred lines was 0.13–1.48, 0.017–0.145, 0.013–0.131, and 0.0001–0.0408?mg?As?kg^?1, respectively. The inbred lines with low As concentrations in the kernels perhaps could be used as a parent to select an elite hybrid with low As concentration for decreasing the As damage for people and animal.     

Effects of different organic materials on forage yield and nutrient uptake of silage maize (Zea mays L.)

The use of organic materials as a source of nutrients on agricultural lands ameliorates soil physical properties as well as being an environmentally friendly way of disposing of their wastes. This study was conducted to determine effects of three organic materials (poultry litter, cattle manure, leonardite) on yield and nutrient uptake of silage maize. Poultry litter and cattle manure were applied based on phosphorus (P) or nitrogen (N) requirements of the crop whereas leonardite was applied only one dose (500 kg ha^?1) and also combined with three inorganic fertilizer doses (100%, 75%, 50% of recommended inorganic fertilizer dose). According to the results, the highest green herbage yield and nutrient uptake values were observed in LEO-100 whereas N-based treatments significantly decreased yield and nutrient uptake of silage maize. The use of organic materials as a combination with inorganic fertilizer in silage maize cultivation is highly beneficial for sustainable forage production.     

镉对玉米苗中钙调蛋白含量和Ca2+-ATPase 活性的影响

试验采用营养液培养的方法,以玉米为试材,研究了不同供镉浓度（0、5、20和100μmol／L）和处理时间（12、2,4、48、96、168h）对植株体内钙调蛋白（CaM）含量及生物膜上的Ca^2＋＋ATPase活性的影响。结果表明,植株可溶性Ca^2＋含量在镉胁迫后较不加镉处理增加,镉处理在叶和根中分别在48和24h后达最高,然后随镉处理浓度和处理时间的增加逐步下降;同时镉诱导了植株CaM的合成,其含量随镉处理浓度和处理时间增加逐步增加,但20μmol／L和100μmol／L镉处理在168h后有所下降;与不加镉处理相比,镉胁迫导致植株生物膜上的Ca^2＋-ATPase活性迅速升高,但随镉处理浓度提高和时间延长,镉胁迫植株的Ca^2＋-ATPase活性在48h（质膜、液泡膜和内质网膜）和24h（线粒体膜）后逐步降低。各膜上的Ca^2＋-ATPase活性依次为质膜〉液泡膜〉内质网膜〉线粒体膜,且同一微囊膜,根中的活性大于叶中。     

Hand planter for maize (Zea mays L.) in the developing world

Third world maize (Zea mays L.) production is characterized by having extremely low yields, attributed in part to the poor planting methods employed. Maize planting in most third world countries involves placing 2–3 seeds per hill, with hills being roughly 30 cm apart. The variability in seeds per hill and distance between hills result in heterogeneous plant stands that are directly responsible for lower yields. Oklahoma State University (OSU) has developed a durable hand planter with a reciprocating internal drum that delivers single maize seeds per strike and that can also be used for mid-season application of urea fertilizer. The hand planter is 1.4 m in length, 5.8 cm in diameter, and weighs 1.9 kg when empty. The seed hopper has the capacity to hold 1 kg of seed and the tip has a sharp pointed shovel which can deliver seed to a planting depth of 5 cm in no-till and tilled soils. The current prototype has been comprehensively tested and evaluated to deliver at least 80% single seeds (singulation), with 0% misses and work well across varying seed sizes (2652–4344 seeds/kg) and different operators. Using the OSU hand planter, third world maize producers with average yields of 2.0 Mg ha^?1 could increase yields by 20%.     

Diversity of diazotroph populations in the rhizosphere of maize (Zea mays L.) growing on different French soils

Summary We studied the dominant diazotrophs associated with maize roots and rhizosphere soil originating from three different locations in France. An aseptically grown maize plantlet, the spermosphere model, was used to isolate N₂-fixing (acetylene-reducing) bacteria. Bacillus circulans was the dominant N₂-fixing bacterium in the rhizosphere of maize-growing soils from Ramonville and Trogny, but was not found in maize-growing sandy soil from Pissos. In the latter soil, Enterobacter cloacae, Klebsiella terrigena, and Pseudomonas sp. were the most abundant diazotrophs. Azospirillum sp., which has been frequently reported as an important diazotroph accociated with the maize rhizosphere, was not isolated from any of these soils. The strains were compared for their acetylene-reducing activity in the spermosphere model. The Bacillus circulans strains, which were more frequently isolated, also exhibited significantly greater acetylene-reducing activity (3100 nmol ethylene day^-1 plant^-1) than the Enterobacteriaceae strains (180 nmol ethylene day^-1 plant^-1). This work indicates for the first time that Bacillus circulans is an important maizerhizosphere-associated bacterium and a potential plant growth-promoting rhizobacterium.     

Silicon alleviates the toxicity of cadmium and zinc for maize (Zea mays L.) grown on a contaminated soil

Although silicon (Si) is not an essential element, it presents a close relationship with the alleviation of heavy‐metal toxicity to plants. This work was carried out to evaluate the effects of Si application to soil on the amelioration of metal stress to maize grown on a contaminated soil amended with Si (0, 50, 100, 150, and 200 mg kg^–1) as calcium silicate (CaSiO₃). Additionally, the cadmium (Cd) and zinc (Zn) bioavailability as well as their distribution into soil fractions was also studied. The results showed that adding Si to a Cd‐ and Zn‐contaminated soil effectively diminished the metal stress and resulted in biomass increase in comparison to metal‐contaminated soil not treated with Si. This relied on Cd and Zn immobilization in soil rather than on the increase of soil pH driven by calcium silicate application. Silicon altered the Cd and Zn distribution in soil fractions, decreasing the most bioavailable pools and increasing the allocation of metals into more stable fractions such as organic matter and crystalline iron oxides.     

Root zone selenium reduces cadmium toxicity by modulating tissue-specific growth and metabolism in maize (Zea mays L.)

The effects of selenium (Se) cadmium (Cd) interactions on plant growth and metabolism are not fully clear. In the present study, we assessed whether Se could alleviate the toxic effects of Cd on growth and metabolism of maize. Seeds of maize variety FH-985 were sown in pots filled with sand treated with CdCl₂ (0, 50 and 100 µM) and Se (0, 2 and 4 mg L^?1) through Hoagland’s nutrient solution. Low Se (2 mg L^?1) increased germination percentage and rate, while high Se (4 mg L^?1) increased fresh and dry biomass under Cd stress. Interestingly, all Se concentrations were effective in alleviating the toxic effects of Cd on photosynthetic pigments, whereas higher Se mitigated the Cd-induced oxidative stress and increased flavonoids both in the shoots and roots while phenolics in the roots. The results demonstrated that root zone Se altered tissue-specific primary metabolism in maize. Furthermore, low Se mitigated the Cd-induced decrease in total proteins in the root. Overall, Se-mediated decrease in the oxidative stress in the shoots while increase of secondary metabolites in the roots helped the plants to grow faster at early growth stage and caused increase in the biomass under different Cd regimes.     

Tillage and dicyandiamide influence on nitrogen fertilizer immobilization,remineralization, and utilization by maize (Zea mays L.)

Summary Both tillage and nitrification inhibitors such as dicyandiamide (DCD) have the potential to influence N availability and thus plant N uptake. A field experiment was conducted to investigate the impact of DCD and tillage (rototillage and no-tillage) on N immobilization and the subsequent impact of residual and fertilizer N on N availability to maize (Zea mays L.) ¹⁵N-labeled urea and urea-DCD were surface applied at the rate of 16 g N m^-2, in either 1987 or 1988, to small plots which had been planted to maize (Zea mays L.). Soil samples were collected four times during the 1988 growing season and analyzed for the ¹⁵N and ¹⁴N components of inorganic N, organic N, and hydrolyzable (6 M HCl) amino acid N, hydrolyzable NH _inf4 ^sup+ -N, and non-hydrolyzable N. Plant samples were collected three times during the 1988 growing season, and analyzed for the ¹⁵N and ¹⁴N components of total N. The total amount of NO _inf3 ^sup- percolating through the profile was less than 15 kg N ha^-1 in 1987 and 1988. N uptake by maize was reduced under notillage and when the urea was treated with DCD. The tillage treatments had no effect on the uptake of N fertilizer applied in 1988 or on N immobilization. However, no-till-age reduced the uptake of residual N fertilizer. The reduced use of N fertilizer was attributed to a reduction in the actual mineralization rates of immobilized residual N. DCD reduced the uptake of N fertilizer applied in 1988. The reduced uptake was attributed to increased N immobilization or to organic matter fixation.