Do elevated soil concentrations of metals affect the diversity and activity of soil invertebrates in the long‐term?

This study aimed to elucidate the response of diversity and activity of soil invertebrates to elevated soil metal concentrations that were a result of sewage sludge application. Field sampling of soil invertebrates was carried out from 2002 to 2004 at an experimental site established in 1982 to test the effects on crop production of metal contamination from sewage sludge applications with elevated concentrations of zinc (Zn), copper (Cu) and nickel (Ni) with certain treatments exceeding the current UK statutory limits for the safe use of sludge on land. At metal concentrations within the limits, none of the invertebrates sampled showed adverse effects on their abundance or overall community diversity (from Shannon–Weiner index). At concentrations above the limits, individual taxa showed sensitivity to different metals, but overall diversity was not affected. Earthworm abundance was significantly reduced at total Cu concentrations at and above 176 mg kg^?1, while nematode and enchytraeid abundances were sensitive to Cu and high Zn concentrations. Correspondingly, litter decomposition was lower in Zn and Cu treatments although there was no direct relationship between decomposition and soil invertebrate abundance or diversity. Such enduring changes in both soil biodiversity and biological activity around the current UK regulatory limits warrant further investigation to determine whether they indicate detrimental damage to soil functioning over the long‐term.     

Characterization and antioxidant activity of the complex of tea polyphenols and oat β-glucan

Few data are available about the effects of complexation of polyphenols with polysaccharide on their bioavailability. The complex of tea polyphenols (TP) with oat β-glucan was characterized by ultraviolet-visible spectrometry, Fourier transform infrared spectrometry, differential scanning calorimetry, atomic force microscopy, and solid-state (13)C NMR spectroscopy. The results indicated that the bonds which governed the interaction between TP and oat β-glucan were strong hydrogen bonds. The in vitro antioxidant activity of TP, β-glucan, their complex, and physical mixture was assessed using four systems, namely, DPPH(?), OH(?), and O(2)(?-) scavenging activities and reducing power. The complexation and blending of TP and β-glucan exhibited different impacts on the index of in vitro and in vivo antioxidant capacities. In the concentration range of 0.5-2.5 mg mL(-1), the complex had highest O(2)(?-) scavenging activity, whereas the highest OH(?) scavenging activity was found with the physical mixture. For antioxidant testing in vivo, there was no significant difference between the complex and the physical mixture in terms of glutathione peroxidase activity and levels of malondialdehyde and total antioxidant capacity in serums. However, the complex exhibited much higher activities of superoxide dismutase and glutathione peroxidase in livers than the physical mixture. The present study provided a deeper understanding of the influence of molecular interaction between TP and oat β-glucan on their antioxidant activities.     

Method of studying the degradation of soil macroaggregates based on the self-absorption of 137Cs β radiation

A method was proposed for studying the degradation of soil aggregates in time from the absorption of ¹³⁷Cs β-radiation by a soil sample. The method is based on the difference in the recorded β radiation intensities depending on the distribution of ¹³⁷Cs between the surface of the aggregates and the nonaggregated soil material. At the localization of the radionuclide on the surface of the soil aggregates, the counting rate will significantly exceed that for the samples containing a similar amount of the radionuclide uniformly distributed throughout the soil volume. This effect is due to the insignificant range of the mild β radiation in the soil (less than 1 mm depending on the radiation energy). Relatively clear calibration graphs under the selected conditions of the radiometric measurements were obtained for the aggregates whose sizes were in the range of 3–10 mm. Under natural conditions, the 7- to 10-mm aggregates of a dark gray clay loamy soil under a forest belt decomposed by 50–65% at a depth of 10 cm and by 23–32% at a depth of 30 cm. The more intense degradation of the soil aggregates of the same size was observed under the conditions of a pot experiment.     

Effect of soil amendments on fractionation of Selenium‐enriched soil

Abstract

This study was to determine the effect of soil amendments on the fractionation of selenium (Se) using incubation experiments under simulated upland and flooded conditions. The treatments were as follows: 1) control [soil + sodium selenite (Na₂SeO₃) (1 mg Se kg^‐1)]; 2) control + calcium carbonate (CaCO₃) (5 g kg^‐1); 3) control + alfalfa (40 g kg^‐1); and 4) control + CaCO₃ (5 g kg^‐1) + alfalfa (40 g kg^‐1). After a 90‐day incubation, soil was sampled and fractionated into five fractions: 1) potassium sulfate (K₂SO₄)‐soluble fraction (available to plants); 2) potassium dihydrogen phosphate (KH₂PO₄)‐exchangeable fraction (potentially available); 3) ammonium hydroxide (NH₃H₂O)‐soluble fraction (potentially available); 4) hydrochloric acid (HCl)‐extractable fraction (unavailable); and 5) residual fraction (unavailable). Compared with the control, CaCO₃ increased the K₂SO₄ fraction at the expense of the NH₃H₂O fraction. Alfalfa increased both the K₂SO₄ and residual fractions but reduced the KH₂PO₄ and NH₃H₂O fractions. The CaCO₃‐alfalfa treatment had a similar effect to the alfalfa treatment alone. The comparison between the upland and flooded conditions showed that the flooded condition generally increased the residual fraction and decreased the potentially‐available fractions. In general, CaCO₃ was a better amendment because it not only increased the available fraction but also maintained the potentially available fractions at a high level. The application of Na₂SeO₃ and use of appropriate soil amendments can improve Se availability in soil.     

Changes in the salinity of solonetzic soil complexes of the Ergeni Upland under long-term anthropogenic impact (soil studies at the Arshan’-Zel’men Experimental Station of the Russian Academy of Sciences)

Unique experiments performed since the 1950s at the Arshan’-Zel’men Experimental Station have formed the basis for afforestation in the dry steppe and semidesert zone without irrigation on the salt-affected soils of solonetzic soil complexes of the Ergeni Upland. Ameliorative measures favored the accumulation of productive moisture in the upper 2-m-thick soil layer, which ensured the growth of trees and the partial leaching of soluble salts to a depth of 1–1.4 m. However, no complete desalinization of the soil profiles took place. The degree of removal of exchangeable sodium from the exchange complex (soil dealkalization) was smaller. The monitoring of changes in the salt status of the soils upon agroforest reclamation was performed until the early 1980s. Our investigations of 2005–2006 showed that the soil amelioration is still in progress: the salt maximum in the profile of the solonetzes descended to a depth of 2.2 m, and the exchangeable sodium was lost from the plow layer (0–40 cm). Plowed soils between forest shelterbelts were also subjected to desalinization and dealkalization of their soil profile, though less intensely than those under the shelterbelts.     

Nitrification and denitrification in an acidic soil of tea (Camellia sinensis L.) field estimated by δ15N values of leached nitrogen from the soil columns treated with ammonium nitrate in the presence or absence of a nitrification inhibitor and with slow-release fertilizers

Forty-two-day-old wheat (Triticum aestivum L. var. Asakazekomugi) plants were treated with complete, K-free (—K), Ca-limited (—Ca), and Mg-free (—Mg) nutrient solutions for 10 days using 2 mM NH₄NO₃ as the nitrogen source, which was replaced with 4 mM ¹⁵ NH₄C1 or Na¹⁵NO₃ for the subsequent 2 days to investigate the absorption, translocation, and assimilation of inorganic nitrogen in relation to the mineral supply. In another experiment plants were grown on NO₃ ^?, NH₄ ⁺, NH₄N0₃, and K-free and Ca-limited NH₄N0₃ nutrient solutions for 10 days, and then in the latter three treatments the nitrogen source was replaced with NO₃ ^? and half of the —K plants received K for 6 days to examine the changes in the nitrate reductase activity (NRA).

Wheat plants absorbed NH₄ ^?N and NO₃-N at a similar rate. Influence of K on the absorption of N0₃-N was stronger than that on the absorption of NH₄-N in wheat plants. The supply of K to the —K plants increased the absorption of NO₃-N, while the absorption of NH₄-N still remained at a lower rate in spite of the addition of K. A limited supply of Ca and lack of Mg in nutrient media slightly affected the absorption of NH₄-N. The influence of K was stronger on the translocation of nitrogen from roots to shoots, while Ca and Mg had little effect. When K was supplied again to the —K plants the translocation of NO₃,-N was more accelerated than that of NH₄-N. Incorporation of NH₄-N into protein was higher than that of NO₃-N in all the tissues; root, stem, and leaf. Assimilation of NH₄-N and NO₃-N decreased by the —K and —Mg treatments.

Leaf NRA of wheat plants decreased in the —K and —Ca plants. Higher leaf NRA was found when K was given again to the —K plants than when the plants were continuously grown in K-free media. Replacement of NO₃ ^? with NH₄ ⁺ as the nitrogen source caused a decline of leaf NRA, while the supply of both NH₄ ^?N and NO₃-N slightly affected the leaf NRA.     

Physical properties of soils and the simulation of the hydrothermal regime for the complex soil cover of the Vladimir Opol’e region

A methodological approach to the physically sound mathematical simulation of the hydrothermal regime for a complex soil cover on an agricultural field scale was proposed. To realize the approach, it is necessary (1) to restore the hydrothermal regime of two contrasting soils using a physically sound mathematical model on the basis of the experimental thermophysical and hydrophysical characteristics, (2) to adapt and optimize the model using the available experimental regime data, (3) to determine the hydrophysical and thermophysical properties for the entire plot under study using pedotransfer functions, and (4) to restore the hydrothermal regime of the entire soil plot using a mathematical model and the meteorological data for a specific time period. The proposed procedure allows simulating the hydrothermal regime of an agrogray soil complex in the Vladimir Opol??e region with a normalized standard error of about 8%. The analysis of the hydrothermal regime for the soil cover of the studied plot calculated from the meteorological data for the period from May to August of 2009 showed that the lower temperature values were confined to the areas of the agrogray soils with the second humus horizon: their average temperature was lower than the temperature of the agrogray soils by 0.44, 0.93, and 1.32°C at depths of 20, 40, and 70 cm, respectively, and the differences between their sums of the active temperatures for the considered period of 2009 reached 89 and 74°C at depths of 20 and 40 cm, respectively.     

A method for the determination of β-glucosidase activity in soil

A method is described for the rapid and simple assay of soil β-glucosidase activity. It involves colorimetric estimation of ρ-nitrophenol released by β-glucosidase activity when soil is incubated in McIlvaine buffer (pH 4.8) with ρnitrophenyl βd-glucoside and toluene at 30°C for 1 hr. The method has been applied to three different soils. The range of β-glucosidase activity in cultivated soils was from 10.1 to 15.2 mµ mole per min per gram of dried soil. K_m value for ρ-nitrophenyl β-d-glucoside was 3.3 × 10^-4 M. Optimum pH was 4.8.     

Mineralization of native soil organic matter is not regulated by the size,activity or composition of the soil microbial biomass—a new perspective

Soil organic matter is extensively humified; some fractions existing for more than 1000 years. The soil microbial biomass is surrounded by about 50 times its mass of soil organic matter, but can only metabolize it very slowly. Paradoxically, even if more than 90% of the soil microbial biomass is killed, the mineralization of soil organic matter proceeds at the same rate as in an unperturbed soil. Here we show that soil organic matter mineralization is independent of microbial biomass size, community structure or specific activity. We suggest that the rate limiting step is governed by abiological processes (which we term the Regulatory Gate hypothesis), which convert non-bioavailable soil organic matter into bioavailable soil organic matter, and cannot be affected by the microbial population. This work challenges one of the long held theories in soil microbiology proposed by Winogradsky, of the existence of autochthonous and zymogenous microbial populations. This has significant implications for our understanding of carbon mineralization in soils and the role of soil micro-organisms in the global carbon cycle. Here we describe experiments designed to determine if the Regulatory Gate operates. We conclude that there is sufficient experimental evidence for it to be offered as a working hypothesis.     

Calibration of the time‐domain reflectometer and determination of the volumetric water content of the soil profile in an ultisol of Costa Rica

Abstract

An experiment was conducted to determine if time‐domain reflectometry (TDR) could be used to measure the water content at different depths in the O‐to‐75 cm soil layer. Probes of three wires (1/8 inch diameter and 30 cm exposed length) were installed in field plots differing in current crop‐fertilization history. Measurements of volumetric water content using bulk density and gravimetric water content were made to calibrate the TDR method. Comparison of water contents determined by TDR with those from gravimetric samples showed that there is a linear relationship (small offset but same slope) of water content with depth, indicating that there is little difference in volumetric water content from the 0 to 75 depth. However, the TDR method gives consistently lower water content values as compared with values obtained by gravimetric determination. Continuous measurements of profile soil water content with TDR in wet and dry periods during the year indicated that the mayor differences in volumetric water content correspond to the first 30 cm depth.     

Can sieving size affect the determination of soil available micronutrients?

<正>Dear Editor,Globally, approximately two billion people suffer from micronutrient deficiencies(Tulchinsky, 2010; Myers et al.,2014).Soil micronutrient availability is of great importance for the evaluation of soil fertility and the determination of appropriate measures for improving crop quality and human health.The diethylenetriamine-penta-acetic(DTPA)     

Molecular modeling of soil organic matter: Squaring the circle?

The structure of soil organic matter (SOM) and humic substances (HS) has been discussed from different viewpoints including molecular conformation, molecular aggregation, macromolecularity, supramolecular characteristics, domain mobility, and many others. Until now, the individual models appear partly contradictory, although each viewpoint provides important information on the structural and functional properties of SOM. This is most probably due to the huge heterogeneity of SOM. Therefore, the question: “How can molecular modeling help to further understand structure and functioning of soil organic matter?” needs to be addressed with care. This contribution reviews and discusses the potential of important molecular modeling approaches currently applied in soil organic matter science.Computer models are useful in giving a visualization of the general structure and of the possible effects on soil chemistry and soil physics. Computational chemistry in this context aims to estimate a lowest energy conformation for a molecule or an assembly of molecules specified by the programmer. On the basis of the calculated conformation, physicochemical characteristics like surface area, polarity and other can be estimated and information on the stability of molecular assemblies can be derived. The significance of the obtained conformation and physicochemical information strongly depends on the initial hypothesis of the molecular structure of each involved molecule. Recent computer models have been developed on the base of computer assisted structure elucidation (CASE). In this procedure, all possible isomers or a statistically representative set of isomers consistent with the experimental input data are processed.Further interesting fields of computational chemistry in soil research follow a different conception, where specific processes of interest are elucidated with the help of computational models which simplify the humic molecules with respect to the individual modeling problem. This way helps to understand the relevance of principal processes expected to occur in soil. In this context, complexes of Al with organic acids, clay mineral sorption sites, interactions of pesticides with organic functional groups or organic soil constituents as well as cross-linking of molecule segments by water molecules were modeled in targeted process-orientated models. The act of simplification is the crucial process in these kinds of models, and if the models are based on good conceptions, they allow to learn about potential SOM functioning. The transfer to more complex situations, however, needs special care and the predictive character of these models needs to be judged with care. Still, any computer model is only as good as its initial hypothesis.     

Effect of vegetation changes on soil erosion on the loess plateau

Vegetation is one of the key factors affecting soil erosion on the Loess Plateau. The effects of vegetation destruction and vegetation restoration on soil erosion were quantified using data from long-term field runoff plots established on the eastern slope of the Ziwuling secondary forest region, China and a field survey. The results showed that before the secondary vegetation restoration period （before about 1866-1872）, soil erosion in the Ziwuling region of the Loess Plateau was similar to the current erosion conditions in neighboring regions, where the soil erosion rate now is 8 000 to 10 000 t km^-2 year^-1. After the secondary vegetation restoration, soil erosion was very low; influences of rainfall and slope gradient on soil erosion were small; the vegetation effect on soil erosion was predominant; shallow gully and gully erosion ceased; and sediment deposition occurred in shallow gully and gully channels. In modern times when human activities destroyed secondary forests, soil erosion increased markedly, and erosion rates in the deforested lands reached 10 000 to 24000 t km^-2 year^-1, which was 797 to 1682 times greater than those in the forested land prior to deforestation. Rainfall intensity and landform greatly affected the soil erosion process after deforestation. These results showed that accelerated erosion caused by vegetation destruction played a key role in soil degradation and eco-environmental deterioration in deforested regions.     

Comparison of the results of soil profiles’ diagnostics performed in three classification systems

Three soil classification systems—the World Reference Base for Soil Resources (WRB), Soil Taxonomy, and the recent Russian system—were used for the identification of 17 soil profiles in southwestern Poland; all the systems put emphasis on the soil properties as diagnostic criteria. Different soils developed on glaciofluvial plains, loessic uplands, and in the Sudetes Mountains were classified. The best correlation between the classification decisions in the different systems was obtained for the most widespread soils owing to the similarity of the diagnostic criteria, which were essentially close although not coinciding. The most prominent divergence between the systems in both the names and the taxonomic categories of the soils was found for the polygenetic soils and for the soils developing from the lithologically discontinuous parent materials. It was also found that the diagnostic elements differ in terms of their taxonomic importance among the classification systems.     

Meeting the challenge of scaling up processes in the plant–soil–microbe system

The scaling up of processes in the plant–soil–microbe system represents one of the greatest challenges facing environmental scientists and yet is essential for sustainable land management worldwide. The latter encompasses, for example, the mitigation of and adaptation to anthropogenic climate change, the bioremediation of industrially contaminated sites, catchment management of human pathogens such as Escherichia coli O157 and integrated crop management on the farm. Scaling up is also essential for the regional and global biogeochemical modelling that will inform policy-makers of the critical environmental factors driving climate change. Despite increasing understanding of the links between gene expression and process on a microscale, there is still much progress to be made when relating this to processes at the macroscale. In this paper, we explore the challenges this poses and examine key case studies of successful up-scaling.

Radioecological description of the soil–plant cover and water objects within the impact zone of the Novovoronezh Nuclear Power Plant

The work considers the accumulation and redistribution of ¹³⁷Cs and ⁶⁰Co among the components of natural ecosystems (soils, bottom sediments, surface waters, and plants of different ecological groups (mesophytes, hygrophytes, hydrophytes, and hydathophytes)) within the impact zone of the Novovoronezh Nuclear Power Plant. It is shown that the current radioecological situation on this territory is satisfactory. Each of the studied parameters complies with the radiation safety standards.     

Can root exudate components influence the availability of pyrene in soil?

Purpose

Little information is currently available regarding the influence of different root exudate components (RECs) on the availability of persistent organic pollutants in the soil environment. In this study, we investigated the impacts of different RECs including organic acids, amino acids, and fructose on the availability of pyrene as a representative polycyclic aromatic hydrocarbon (PAH) in soils.

Materials and methods

Citric acid, oxalic acid, malic acid, serine, alanine, and fructose were used in the experiments as representative RECs. Pyrene-spiked soils (TypicPaleudalfs) with present RECs were incubated for 30 days, and the available fraction of pyrene was determined using n-butanol extraction procedure.

Results and discussion

The amount of n-butanol-extractable pyrene in soil increased with the addition of tested RECs and increased when REC concentrations are enhanced within the range of 0–21 g kg^?1. The extractability of pyrene in soil with REC treatments and the enhancement ratio (r, %) of the extractable pyrene in soil by the addition of RECs after a 30-day incubation decreased in the following order: organic acids (oxalic acid ≥ citric acid > malic acid) > amino acid (alanine > serine) > fructose. This decrease was observed irrespective of soil sterilization, although the concentrations of extractable pyrene were lower in non-sterilized soils compared to sterilized soils. The concentrations of metal cations and dissolved organic matter (DOM) in solution increased when organic acids were added.

Conclusions

The tested RECs at concentrations of 0–21 g kg^?1 clearly enhanced the availability of pyrene in soils, and larger amounts of RECs resulted in higher pyrene availabilities in the tested soils. Microbial biodegradation diminished the amount of available pyrene irrespective of the presence of RECs. The mechanism of REC-influenced availability of pyrene in soil may be related to the metal dissolution and release of DOM from soil solids. The results of this study will be useful in assessing PAH-related risks to human health and the environment and will be instructive in food safety and remediation strategies at contaminated sites.     

BODIUM—A systemic approach to model the dynamics of soil functions

The increasing demand for biomass for food, animal feed, fibre and bioenergy requires optimization of soil productivity, while at the same time, protecting other soil functions such as nutrient cycling and buffering, carbon storage, habitat for biological activity and water filter and storage. Therefore, one of the main challenges for sustainable agriculture is to produce high yields while maintaining all the other soil functions. Mechanistic simulation models are an essential tool to fully understand and predict the complex interactions between physical, biological and chemical processes of soils that generate those functions. We developed a soil model to simulate the impact of various agricultural management options and climate change on soil functions by integrating the relevant processes mechanistically and in a systemic way. As a special feature, we include the dynamics of soil structure induced by tillage and biological activity, which is especially relevant in arable soils. The model operates on a 1D soil profile consisting of a number of discrete layers with dynamic thickness. We demonstrate the model performance by simulating crop growth, root growth, nutrient and water uptake, nitrogen cycling, soil organic matter turnover, microbial activity, water distribution and soil structure dynamics in a long-term field experiment including different crops and different types and levels of fertilization. The model is able to capture essential features that are measured regularly including crop yield, soil organic carbon, and soil nitrogen. In this way, the plausibility of the implemented processes and their interactions is confirmed. Furthermore, we present the results of explorative simulations comparing scenarios with and without tillage events to analyse the effect of soil structure on soil functions. Since the model is process-based, we are confident that the model can also be used to predict quantities that have not been measured or to estimate the effect of management measures and climate states not yet been observed. The model thus has the potential to predict the site-specific impact of management decisions on soil functions, which is of great importance for the development of a sustainable agriculture that is currently also on the agenda of the ‘Green Deal’ at the European level.