不同氮、磷施用量对甜菜叶片中蔗糖代谢有关酶活性的影响

甜菜叶片中蔗糖磷酸合成酶、蔗糖合成酶、转化酶的活性随土壤施氮、磷的量不同而变化,氮磷营养对蔗糖磷酸合成酶的活性调节表明,随施氮量的增加（0～300kg/hm^2）,叶片蔗糖磷酸合成酶的活性相应地提高,在同等施氮条件下,增加磷的施用量可提高蔗糖磷酸合成酶的活性.在苗期,施用氮、磷肥对蔗糖合成酶的活性没有影响.生育后期,蔗糖合成酶的活性随施氮量的增加而活性提高.但在较高的施氮水平下,提高磷的施用量可降低蔗糖合成酶的活性.生育期内氮、磷对转化酶无明显的调节作用.     

Nitrogen compounds in organs of two sugar beet genotypes (Beta vulgaris L.) during the season

A major aim in sugar beet breeding is to decrease the concentration of soluble N compounds in the beet to avoid negative impacts during beet processing. Due to its importance and analytical limitations the only selection criterion is amino N analyzed in the beet at harvest. It is thus not clear when genotypic differences are established during the season. Furthermore, it is not known whether selection for low amino N affects other N compounds in the beet and the N composition of other plant organs. Therefore, the concentrations of total N and soluble N compounds (protein, amino N, glutamine, betaine, nitrate) were investigated during the season in different organs (leaf blades, petioles, crown, beet) of two sugar beet genotypes differing in the amino N concentration of the beet. Field trials were carried out at three sites in 2002 and in 2003 with harvests at three times from 100 to 170 days after sowing.     

不同土壤环境对甜菜产质量的影响

试验结果表明,非病地比病地平均提高根产量、含糖率、产糖量分别为12230.6kg/hm、4.46度、3122.4kg/hm2;t测验分析表明,不同土壤环境对甜菜产质量具有不同程度的影响,其大小顺序是含糖率>产糖量>根产量,分别有94.1%、84.7%和50.7%的材料因不同土壤环境而达到显著或极显著差异。方差分析结果显示,在病地参试材料的根产量、产糖量的差异显著性与非病地的基本同步,而含糖率差异显著性却不完全同步,病地更能造成材料间含糖率的显著或极显著差异。两种地块导致单胚材料、饲料甜菜的根产量和产糖量差异悬殊。     

氮素不同用量对甜菜产质量的影响

氮素的不同用量对甜菜的块根产量、含糖率、产糖量均有一定影响。通过试验得出,当甜菜施氮量达到180kg/hm2时,甜菜块根产量、含糖率、产糖量最高,分别为35038.5kg/hm2、17.2%、5073kg/hm2。     

Effect of nitrogen fertilization on the nutritional quality of wheat variety,Yecora Rojo

The effects of N-fertilization on the nutritional quality of short-straw wheat variety, Yecora Rojo, was studied under sprinkler irrigation system. The fertilizers applied were the conventional N-fertilizer, urea (U) applied as single dose or in three split applications (U-S), and the slow release N-fertilizers, ureaform (UF) (38% N and 20 N), osmocote (OC) with 9 or 14 months longevity and sulphur-coated urea (SCU) with either 17% or 37% dissolution rates in 7 days. These fertilizers were applied at 0 (control), 75, 125, and 175 kg/ha during the 1982–1983 season. Results indicated that at 75 kg/ha there was no significant change in the protein content. At 125 kg/ha, the protein content increased (P<0.05) with urea (applied in three split applications) and OC. At 175 kg/ha, all the fertilizers increased the protein content with the exception of urea (applied as single dose) and UF. Ash content tended to decrease with increasing levels of N, while fat and crude fiber were unaffected. All the fertilizers increased the protein yield (kg/ha), the maximum increase occurring with the slow-release N fertilizers, OC and SCU. Except UF, all the fertilizers decreased the lysine content significantly (P<0.05) in the protein moeity at 175 kg/ha level but lysine percentage in the grain was not reduced. The yield (kg/ha) of individual amino acids including lysine was increased several times at the 175 kg/ha level. P content tended to decrease while Fe and Zn increased with fertilization. Slow-release N-fertilizers, OC and SCU as well as urea (in 3 split applications) appear to have a significant positive effect on the nutritional quality of the grains of wheat variety, Yecora Rojo.     

Direct application of reduced rates of aldicarb to potato seedpieces

Aldicarb was applied directly to potato seedpieces at 0.3, 0.6, 1.1, 2.2 and 3.4 kg AI/ha at planting and at 2.24 kg Al/ha over the plants at crop emergence. All seedpiece treatments resulted in superior early season protection from Colorado potato beetle,Leptinotarsa decemlineata (Say). Lower seedpiece rates lost effectiveness by early July, while higher rates protected foliage into early August. In a second study, plots treated with 1.1 kg AI/ha to the seedpiece produced crop yields as high as those treated with additional aldicarb at crop emergence. It is suggested that low rates of aldicarb applied directly to the seedpiece at planting could offer effective early season insect control while reducing the risk of groundwater contamination.     

用基因操作法改良糖甜菜（英）

甜菜的产糖量占世界蔗糖产量的４０％，从碳同化角度来说，甜菜是极高效的温带作物之一，但用基因操作法仍有提高其产质量的潜力。对甜菜贮藏器官发育过程的解剖、生理和蔗糖积累的研究，为制定增加产量和减少环境对甜菜生长影响的策略奠定了理论基础。为促进对发育操作过程以达到上述这些目标，必须增强对细胞分裂的调节机制的认识。我们先研究植物激素限定细胞分裂的方式，随后研究调节细胞分裂的一些分子生物学特征。目前，我们已分离和鉴定了一些与甜菜细胞周期相关的基因序列，并用甜菜细胞悬浮培养对这些与细胞分裂调节相关的基因表达进行了研究，细胞分裂调节基因特定位点表达的策略已被确定，进一步优化甜菜转化系统的研究正日趋完善。通过这个途径，有希望获得高根产、高含糖、高汁液纯度和低环境影响的甜菜植株。     

Integrating the effects of climate and plant available soil water holding capacity on wheat yield

In the Mediterranean farming systems of the Western Australian wheatbelt, crop yields are influenced primarily by the amount and distribution of rainfall and the soil's capacity to hold moisture. The wheatbelt's growing season rainfall varies in the range of 200–400 mm (average) and the plant available water holding capacity (PAWC) of soils is generally in the 40–140 mm range. The grain yield of wheat is sensitive to this combination of small rainfall and small storage capacity.In this study, we explore the relationship between yield and PAWC using a combination of simulation modelling and analysis of field data. Crop yields and soil properties were monitored in detail at 17 locations (PAWCs 43–131 mm) across six seasons (1997–2005). Crop yields were also simulated using the APSIM crop simulator (RMSE = 311 kg/ha) to evaluate the long-term relationship between crop yield and plant available water capacity using 106 years of historical climate data.The relationship between crop yield and PAWC varied with season, and two important factors emerged: (1) for PAWC < 65 mm, there was a linear relationship with crop yields that ranged from 17 kg/ha/mm to 58 kg/ha/mm of PAWC across seasons; (2) for PAWC 65–131 mm the crop yield response to PAWC ranged from 11.5 kg/ha/mm in 45% of seasons to no response.The impact of PAWC on crop yield was reduced in seasons with late rainfall, and magnified in seasons with reduced rainfall late in the growing season. Six distinct season types with different yield–PAWC relationships are identified and season-specific management strategies that exploit within-field variation in PAWC are developed to manage the spatial variation of PAWC in a field.     

AMMONIA AS A SOURCE OF NITROGEN FOR GRASS SWARDS

Aqueous ammonia was injected to a depth of 10 cm into small grass plots in late winter at rates equivalent to 200 and 400 kg N per ha. The effect on yield of DM, seasonal distribution of yield and uptake of N in the herbage was compared with that of single or split applications of ammonium nitrate. Two levels of supplementary irrigation were given in summer. The method of injection minimised damage to the sward and loss of ammonia by volatilization.
Aqueous ammonia and ammonium nitrate (single application) gave similar yields of dry matter, uptake of N and distribution of yield over the season. Ammonia was no more effective than ammonium nitrate under dry conditions. A single application in February of 200 kg N/ha as ammonium nitrate gave a greater total annual yield than the same quantity of N as ammonia or as a split dressing of the solid fertilizer. At 400 kg N/ha, split application gave the highest yields. The uptakes of N in the harvested grass was similar, in each irrigation treatment and at each level of N, for the three methods of applying N, The apparent recovery was low.
Aqueous ammonia, efficiently injected, was a satisfactory form of N for grass at the first cut, but it did not provide a continuing supply of N for crops harvested later in that season.     

Chemical weed control in potatoes

Nine herbicide treatments were applied to potatoes grown at three locations in each of 3 years. The locations were characterized by widely different soil types—silty clay loam, sandy loam, and muck—and by differences in rainfall and temperatures during the growing season. None of the herbicide treatments applied pre-emergence gave adequate weed control at all locations and/or in all years. EPTC at 6 lb per acre (6.72 kg/ha) gave excellent weed control on both mineral soils but not on muck. Metabromuron at 4 lb per acre (4.48 kg/ha gave excellent weed control only on the low organic matter sandy loam. Propachlor at 6 lb. (6.72 kg/ha) and 2 lb amiben plus 3 lb propachlor (2.24 plus 3.36 kg/ha) gave variable control. Four herbicides: 1 lb paraquat (1.12 kg/ha), 2 lb linuron (2.24 kg/ha), 6 lb dinoseb (6.72 kg/ha) and 6 lb dinoseb plus 2.25 dalapon (6.72 plus 2.52 kg/ha) applied at potato plant emergence, when weeds were in the 2–6 leaf stage, also gave variable control but as a group generally gave better control than the pre-emergence herbicides, particularly on muck soil. The effectiveness of herbicides applied at potato plant emergence was less affected by soil type than those applied pre-emergence but was affected by the degree of weed emergence. On muck soil, weeds generally emerged well before “at emergence” herbicide treatments were applied. Tuber yields were not directly affected by the herbicides, but were reduced where weed control was inadequate. Tuber specific gravity and chip color were unaffected by the herbicides.     

Influence of different tillage methods and pre-emergence herbicides on weed control in cowpea (Vigna unguiculata (L.) Walp.)

In the early and late cropping seasons of 1983, three different methods of tillage (minimum tillage, conventional tillage, and tilled and ridged) were evaluated for their influence on the performance of some pre-emergence herbicides used during the production of cowpea. Four varieties of cowpea (Ife Brown, H 144-1, TVx 3236, and Black-eyed bean) were planted in the early season while five varieties (Ife Brown, H 144-1, TVx 3236, H64/3 and Vita-5) were planted in the late season. A single spray application of 3·0 kg/ha alachlor, 3·0 kg/ha metobromuron + metolachlor and 3·5 kg/ha metolachlor was carried out one day before planting. Cyanazine at 3·0 kg/ha was included in the late season trial. The plots were either minimally tilled, conventionally tilled or tilled and ridged at the Teaching and Research Farm of the University of Ife. Results showed that the performance of all three herbicides used in the early season, as measured by weed control and grain yields, was best on the conventionally tilled, while the performance of the four herbicides in the late season was best on tilled and ridged plots. Metobromuron + metolachlor performed consistently better than each of the other herbicides irrespective of the method of tillage during both seasons. Conventional tillage enhanced the performance of metobromuron + metolachlor over minimum tillage plots and tilled and ridged plots in the early season; however, in the late season, the performance of metobromuron + metolachlor was best on tilled and ridged plots. Alachlor did not effectively control competing weeds in both seasons but did not injure any cowpea line. On the other hand, cyanazine did not control weeds effectively but was phytotoxic to Ife Brown variety of cowpea. In both seasons, the control of the broad-leaved weeds Ageratum conyzoides, Amaranthus spp., Euphorbia heterophylla, Ipomoea spp., Spigelia anthelmia, Synedrella nodiflora and Talinum triangulare was not adequate. The control of the grassy weeds Brachiaria deflexa, Cynodon dactylon, Imperata cylindrica and Eleusine indica was, however, effective.     

Potato tuber sugar content during development and storage (10°C): possible predictors of storage potential and the role of sucrose in storage hexose accumulation

Summary Reducing sugar and sucrose contents of potato tubers (Solanum tuberosum L.) from six cultivars were monitored both during the growing season and after a four months storage period at 10°C. Significant correlations were found between tuber sugar contents (sucrose, reducing and total) measured at harvest and the reducing sugar content after storage. Similarly, in five of the six cultivars, the sucrose loss and the corresponding reducing sugar gain during storage were significantly correlated.     

The effect of Imidan on the flavor of riced baked potatoes

Samples of Irish potato (Solanum tuberosum) from untreated and Imidan 50-WP—treated plots (four applications, 2.24 kg 50-WP/ha) were evaluated for flavor by a sensory panel of 16 judges. Although the sample means were statistically different (P = 0.05), no off-flavor was associated with the Imidan-treated baked potatoes.     

Influence of potato storage and handling stress on sugars,chip quality and integrity of the starch (amyloplast) membrane

Mechanical handling of Norchip (cv.) potatoes resulted in sucrose concentrations that exceeded 1% (fresh wt basis) 10 days after treatment. More than 65% of the maximal sugar accumulation occurred within 5 days of handling. Potatoes increased in their ability to produce sucrose as storagetime increased. Sucrose accumulation in response to handling stress was not mediated via gross physical disruption of the amyloplast membrane. Electron micrographs indicated that starch granules of both handled and control potatoes were surrounded by intact and continuous plastid (amyloplast) membranes throughout the major portion (1 to 8 months) of the storage season (8.9 C). Glucose levels did not increase at a similar rate compared to changes in sucrose concentration. Results suggest that the majority of the sucrose, which occurred during storage and/or handling stress, was not efficiently converted to reducing sugars via the enzyme invertase. Sucrose and glucose concentrations in all potatoes began to increase and chip color darkened as tubers senesced (9 to 10 months storage). Examination of electron micrographs during this storage period revealed that the bi-layers of the amyloplast membrane were separating. The mechanical handling of senescing potatoes (10 months in storage) resulted in a shift of the plastid membrane to a more disrupted state over that noted in the control. The variation in chip color induced by handling and/or storage time in Norchip potatoes was closely associated with the variation in glucose content (r = 0.953).     

Agronomic and economic evaluation of Sesbania rostrata green manure establishment in irrigated rice

Research on crop establishment methods may improve green manure performance, reduce costs, and increase the adaptability of pre-rice green manure technology in lowland rice-based cropping systems. A two-season field experiment was conducted at the International Rice Research Institute (IRRI) in Los Baños, Philippines in 1991–1992 to compare four establishment practices of Sesbania rostrata green manure (zero tillage, with tillage, relay cropping in rice for 2 or 4 weeks) with four mineral N fertilizer levels (0, 30, 60, and 90 kg urea N/ha) in an intensive irrigated lowland system with three rice crops per year. S. rostrata was grown twice a year during the 43-day dry-wet and wet-dry transition periods between the wet and dry season rice crops.

Grain yield potential and fertilizer responsiveness of rice was generally highest in the dry season. On the other hand, S. rostrata growth was more vigorous in the wet season (long-day period) than in the dry season, regardless of establishment method. Green manure N accumulation was lowest with zero tillage (30 and 90 kg N/ha in dry and wet season, respectively) and highest when it was relay-cropped for two weeks (60 and 180 kg N/ha in dry and wet season, respectively). Land preparation for Sesbania ensured best green manure stand (> 100 plants/m² vs 20–40 plants/m² at no-till establishments) but increased costs of green manuring by US$16/ha compared with other establishment methods. A quadratic response function between mineral fertilizer equivalence and green manure N indicated that up to 75 kg N/ha, lowland rice uses green manure N more efficiently than urea. Depending on season and establishment method, S. rostrata substituted for 35 to 90 kg of split-applied urea N. Benefit-cost ratios indicated that pre-rice green manure use in the wet season under the current fertilizer and labor prices in the Philippines was a less attractive economic option than mineral N fertilizer. This was true for all establishment methods. In the dry season, S. rostrata established by relay cropping gave the highest rate of return. The 2-week relay cropping of green manure with irrigated rice gave highest green manure N accumulation and rice grain yield, and may be economically viable where fertilizer prices are higher or labor costs are lower than in the Philippines.     

中国北方甜菜土壤速效钾丰缺指标与适宜施钾量研究

为了给甜菜测土施肥提供科学依据,采用零散实验数据整合法、缺素处理相对产量与土壤养分含量回归方程法和“养分平衡—地力差减法”确定适宜施肥量新应用公式,开展了我国北方甜菜土壤速效钾丰缺指标与适宜施钾量研究。结果表明,中国北方甜菜土壤速效钾(NH4OAc-K)第1~5级丰缺指标依次为>409、224~409、123~224、68~123和≤68 mg/kg。当钾肥当季利用率40%、目标产量30~90 t/hm^2时,土壤速效钾丰缺级别第1~5级的适宜施钾量范围依次为0~0、41~124、83~248、124~371和165~495 kg/hm^2。     

Effect of three forages and two forage allowances offered to pregnant dry dairy cows in winter on periparturient performance and milk yield in early lactation

Sixty multiparous, Holstein–Friesian pregnant dry dairy cows were allocated to three forage treatments ( n  = 20; fodder beet, kale or grass silage) at two feeding allowances ( n  = 30; high and low) for 70 (s.e. of mean, 16) d before parturition. Cows offered the high feeding allowance were offered 9 kg of dry matter (DM) of kale or fodder beet grazed in situ plus 5 kg DM of baled grass silage daily or clamp grass silage ad libitum offered indoors. Cows offered the low feeding allowance were offered 6 kg DM of kale or fodder beet grazed in situ plus 3·5 kg DM baled grass silage daily, or 9·5 kg DM of clamp grass silage daily offered indoors. After calving, all cows received a daily allowance of 14 kg DM perennial ryegrass herbage at pasture plus 4 kg concentrate cow⁻¹ for the first 35 d of lactation. Cows offered grass silage had a greater increase in body condition score pre-partum compared to those offered kale or fodder beet. Cows offered fodder beet pre-partum had a greater milk solid and solids-corrected milk yield in the first 35 d of lactation than those offered kale and grass silage pre-partum. Offering fodder beet and kale pre-partum increased plasma non-esterified fatty acid concentrations pre-partum relative to offering grass silage. Offering kale pre-partum resulted in higher insulin-like growth factor-1 concentration post-partum but lower plasma copper concentration pre-partum and at calving than kale or grass silage. Offering the higher forage allowance pre-partum resulted in a higher plasma calcium concentration at calving and higher plasma non-esterified fatty acid concentration post-partum.     

POTENTIAL PASTURE PRODUCTION IN THE UPLANDS OF WALES

Resown S23 perennial ryegrass pastures showed considerably greater response to the application of fertilizer N (0·1075 kg N/ha per year = 0–960 Ib N/ac) at 305 m O.D. than the native Festuca ovina/Agrostis tenuis and Molinia caerulea dominant communities on identical brown earth and peaty gley soils. Ryegrass DM production during 1967–70 increased with N application rates up to 538 kg N/ha per year (480 Ib N/ac) on the acid-brown earth, while on the extremely N-deficient gley soil yield responses were recorded up to 1075 kg N/ha (960 Ib N/ac). Percentage N recovery by ryegrass, bowever, although improved by grazing and re circulation, was less than under lowland conditions and the response to N during the growing season was also lower, exceeding 20 kg DM/kg N from applications in May, June and July only. The recovery of N by Festuca/Agrostis and Molinia in 1968–70 ranged from 3% and 2% at 938 kg N/ha to 14% and 9% at 117 kg N/ba per year, respectively. The respective maximum average DM yields recorded were 29 t/ha and 2.2 t/ha from the native communities and 70 t/ha and 62 t/ha from the corresponding resown pastures (2610, 1950, 6250 and 5520 Ib/ac). The results are discussed in relation to the strategy of land improvement in upland areas.     

甜菜钾肥肥效试验

试验结果表明,氯化钾与硫酸钾对甜菜施肥效果相比,没有明显差异。试验以施氯化钾178kg/hm2处理(A1B3)效果最好,与对照比可提高根产、含糖、产糖量分别达到31.0%、2.9度和58.7%。     

甜菜的全息定域选种初探

应用生物全息律,进行了甜菜的全息定域选种试验.结果表明,在甜菜采种株的上部、中部和下部分别采收的种子,其千粒重分别为26.4g、28.5g和31.2g,发芽率分别为93.1%、95.6%和98.3%.将这三个不同部位的种子种植收获后,甜菜块根产量分别为43520.5、43983.5、49539.5 kg/hm2,含糖率分别为12.60%、12.89%和13.22%,产糖量分别为5580.1、5665.0、6520.6 kg/hm2.甜菜生长表现的这些规律,都是由甜菜的生物全息律所决定的,即甜菜全株遗传势的大小分布顺序为上部＜中部＜下部.