The Impact of Underseeding Barley (Hordeum vulgare L.) on Timothy (Phleum pratense L.)-Clover (Trifolium pratense L.; Trifolium hybridum L.) Forage Production in a Cool Maritime Climate

Growing barley (Hordeum vulgare L.) in the year of forage establishment is a common agronomic practice in marginal growing regions, but is often not recommended to growers. We examined the effect of silage barley production over an establishing timothy (Phleum pratense L.)‐clover (Trifolium pratense L.; Trifolium hybridum L.) forage sward in a 4‐year study near St. John's, Newfoundland, Canada. The experiment compared two barley varieties differing in plant height (semidwarf Chapais and Leger), three barley seeding rates and the effect of a forage understorey on forage (barley) production in the establishment year and forage (timothy‐clover) production in the subsequent year. The taller Leger yielded similar forage biomass to Chapais in the year of forage establishment, which (when planted at 375 plants m^?2) was roughly twice that of a pure‐stand timothy‐clover mix. Increasing the barley seeding rate from 125 to 375 plants m^?2 resulted in a linear increase in forage yield in the year of planting. The production of barley forage in the establishment year resulted in reduced timothy‐clover forage yield in the year following barley planted at 125 and 375 seeds m^?2, but not for barley planted at 250 seeds m^?2. Companion planting also altered forage species composition in that higher barley seeding rates resulted in 12–18 % less timothy and 2–4 % lower fibre levels in the year following planting. Barley seeded at rates of 250–375 seeds m^?2 and undersown with a timothy‐clover mixture (harvested at mid‐milk) resulted in greater forage yield of poorer quality than pure‐stand timothy‐clover in the planting year, and a barley seeding rate of 250 seeds m^?2 did not impede forage production in the subsequent year.     

Effects of Soil Moisture Deficit on Forage Quality, Digestibility, and Protein Fractionation of Kura Clover

Kura clover (Trifolium ambiguum M.B.) is a persistent rhizomatous forage legume with great potential for permanent pastures. The effects of a soil moisture deficit on forage quality, digestibility and protein fractionation of two cultivars of Kura clover (Endura and Rhizo) were investigated in this study for 1 year. The responses of alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) were also characterized. Stands of each species were field‐grown and submitted to two soil water regimes promoting soil moisture deficits and well‐watered (i.e. control) conditions. There were no interactions between species and soil water regimes. Soil moisture deficit increased acid detergent fibre (ADF) but reduced acid detergent lignin (ADL) content and consequently increased forage digestibility. It had only minor effects on protein content and fractionation. Species varied for most parameters measured. Kura clover generally had the lowest neutral detergent fibre (NDF), ADF and ADL contents, and consequently the greatest digestibility (83.9 %). Species also differed in their crude protein fractionation. Kura clover and red clover had a lower proportion of non‐protein nitrogen (NPN; A fraction) and a greater proportion of true protein (B fraction) (30.9 and 64.5 %, respectively) than alfalfa (36.4 and 57.4 %, respectively). Kura clover also had the lowest proportion (i.e. 4.7 %) of acid detergent insoluble protein (ADIP; C fraction) of all species tested. Endura Kura clover often had a higher forage quality than Rhizo. This study confirms that Kura clover produces high‐quality forage and provides the first estimates of protein fractionation in this species.     

Hay yield and nitrogen harvest in smooth bromegrass mixtures with alfalfa and red clover in relation to nitrogen application

An experiment was conducted in order to investigate hay yield and nitrogen harvest in binary smooth bromegrass (Bromus inermis Leyss cv. Tohum Islah) mixtures with alfalfa (Medicago sativa L. cv. Kayseri) and red clover (Trifolium pratense L. cv. Tohum Islah) in Erzurum, Turkey for 5 years between 1991 and 1995. The Hay yield, nitrogen harvest, protein concentration and land equivalent ratio (LER) in the mixtures with alternating rows of 1:1, 2:1 and 1:2 of smooth bromegrass with alfalfa and red clover were compared to those in pure legume stands without any N-fertilizer application or pure smooth bromegrass stands that received 0, 50, 100 and 150 kg ha⁻¹ N. The mixtures had no N fertilization apart from 40 kg N ha⁻¹ in the establishment year. The dry matter production in all the mixtures receiving no N fertilizer application was higher than in pure legume stands. Pure grass stands were sustained only with the application of 150 kg ha⁻¹ N. The highest hay yields were obtained from the mixtures of smooth bromegrass (Sb) with red clover (Rc) (2Rc 1Sb) (14.65 t ha⁻¹) and with alfalfa (A) (1A 1 Sb) (14.49 t ha⁻¹). Although N application increased Sb yields in pure stands, the highest yields obtained with N fertilization were still lower than the yields in the mixtures without N application. The superiority of the mixtures was also reflected by their large N harvests (e.g. 355.9 kg N ha⁻¹ in 2Rc 1Sb plots) compared to pure Rc (317.8 kg N ha⁻¹), pure A (294.3 kg N ha⁻¹) and pure Sb stands that received 150 kg N ha⁻¹. The nitrogen harvest increased in pure Sb plots as the N doses applied increased. Furthermore, the protein concentration of the hay from the mixtures (158.2–165.7 mg g⁻¹) was equal to that of the pure A stands (165.7 mg g⁻¹) and higher than that of pure Sb stands (122.9 mg g⁻¹ at 150 kg N ha⁻¹ application) although the hay from pure Rc plots had the highest protein concentration (179.3 mg g⁻¹). The LER values were also higher in the mixtures (e.g. 1.28 in 1A 1Sb and 1.28 in 2Rc 1Sb plots) compared with the pure stands. The mixture plots also had a more balanced temporal distribution of hay. The grass component was more productive in early spring, whereas the legume fractions grew better in the summer. In conclusion, for a sustainable production of high-quality hay and greater N harvests without using N fertilizers, binary mixtures of Sb with A in alternating rows (1A 1Sb) were recommended for long-purpose stands and in alternation with double red clover rows (2Rc 1Sb) for short purpose stands under similar conditions. N application could be eliminated in the grass–legume mixtures without any yield depression.     

Effects of Foliar Application of Elicitors on Red Clover Isoflavone Content

Red clover (Trifolium pratense L.) contains high concentrations of isoflavones, compounds that have received much interest lately due to their presumed benefits for human health. In this experiment we tested the possibility to induce isoflavone production in the foliage of two greenhouse‐grown red clover cultivars (‘Azur’ and ‘Start’) through the application of elicitor compounds. Foliar applications of different concentrations of acetic acid (50, 100, 250 and 500 mm ), yeast extract (1, 2, 3 and 4 g l^?1), and chitosan (125, 250, 500, and 1000 mg l^?1) were carried out on plants at the late vegetative stage, which were harvested 2 or 8 days after spraying. Concentrations of genistein, daidzein, formononetin and biochanin A were determined by high performance liquid chromatography. The two cultivars differed in isoflavone concentrations, ‘Azur’ having on average 36 % higher biochanin A, formononetin and total isoflavone concentrations than ‘Start’ (P < 0.05). A cultivar × sampling date interaction (P < 0.1) reflected a 20 % increase over time in total isoflavone concentration with ‘Azur’, which was not observed with ‘Start’. Effects of elicitors were limited, contrasts indicating overall, 12, 14 and 15 % greater total isoflavone concentration in yeast extract (P < 0.1), chitosan (P < 0.05) and acetic acid (P < 0.05)‐treated plants, respectively, than in untreated control plants. There were few differences between the various elicitors and none between concentrations of each elicitor.     

Effects of species diversity on seasonal variation in herbage yield and nutritive value of seven binary grass-legume mixtures and pure grass under cutting

Intensively managed sown temperate grasslands are generally of low species diversity, although swards based on grass-legume mixtures may have superior productivity and herbage quality than grass-only swards. We conducted a cutting experiment over two years to test the effect of species composition and diversity on herbage yield, contents of N, neutral detergent fibre (NDF) and in vitro organic matter digestibility (IVOMD). Perennial ryegrass (PR, Lolium perenne) was sown alone and with each of four forage legumes: red clover (RC, Trifolium pratense), lucerne (LU, Medicago sativa), birdsfoot trefoil (BT, Lotus corniculatus) and white clover (WC, Trifolium repens); WC was also sown with hybrid ryegrass (HR, Lolium × boucheanum), meadow fescue (MF, Festuca pratensis) and timothy (TI, Phleum pratense). Herbage productivity was lowest in pure PR followed by PR/BT, and highest in PR/RC; this mixture had the highest legume proportion, N content and N yield. There was less WC in swards with HR and MF than with PR and TI. These differences were reflected in N contents of herbage of the mixtures. Legumes had higher N and lignin and lower NDF contents and IVOMD than grasses. Among legumes, NDF content was highest and IVOMD lowest in LU, followed by BT and the clovers. The highest N content was in WC. Among grasses, PR and HR had lower NDF contents and a higher IVOMD than MF; the highest N content was in PR. The grass component of mixtures had less effect than the legume component on herbage yield and quality. Results are discussed in terms of their potential to contribute to forage resources in farming practice and enhance resource use efficiency and ecosystem services.     

Cover crops and interrow tillage for weed control in short season maize (Zea mays)

Weed competition can cause substantial maize (Zea mays L.) yield reductions. Interseeding maize with cover crops or a combination of interrow cultivation and interseeded cover crops are possible alternative methods of weed control. This study was conducted to examine the potential of interrow cultivation plus cover crops to reduce weed density in maize without reducing the grain yield. Field experiments were conducted in 1993 and 1994 at two sites in Québec to determine the effects of planting 12 cover crops with maize on weed control. Fall rye (Secale cereal L.), hairy vetch (Vicia villosa Roth), a mixture of red clover (Trifolium pratense L.) and ryegrass (Lolium multiflorum Lam), a mixture of white clover (Trifolium repens L.) and ryegrass, subterranean clover (Trifolium subterraneum L.), yellow sweet clover (Meliotus officinalis Lam), black medic (Medicago lupulina L.), Persian clover (Trifolium resupinatum L.), strawberry clover (Trifolium fragiferum L.), crimson clover (Trifolium incarnatum L.), alfalfa (Medicago sativa L.), and berseem clover (Trifolium alexandrinum L.) were seeded at two planting dates, 10 and 20 days after maize emergence. Interrow cultivation was carried out weekly until forage seeding, with a final cultivation being conducted just prior to cover crop seeding. Cover crop planting date did not affect maize yields or the ability of interrow tillage plus cover crops to suppress the development of weed populations. Maize yield was less affected by the interseeded cover crops under conditions of adequate rainfall. Corn planted in fields heavily infested with weeds resulted in substantial yield reductions even when rainfall was adequate. Except for 1993 at l'Assomption interrow tillage plus cover crop treatments had consistently lower weed biomass when compared to the weedy control. Most of the weed control was due to the interrow cultivation performed prior to seeding of the cover crops. The lowest weed density occurred in the herbicide treated plots. The ability of interrow tillage plus cover crops to suppress the development of weeds was affected by the level of weed infestation, the growing conditions and location. The cover crops provide additional weed control but the interrrow tillage or some herbicide application may still be necessary.     

Temperature Before Cold Acclimation Affects Cold Tolerance and Photoacclimation in Timothy (Phleum pratense L.), Perennial Ryegrass (Lolium perenne L.) and Red Clover (Trifolium pratense L.)

The expected temperature rise in late summer/early autumn can change the conditions for acclimation and affect the winter survival of perennial crops. This study examined the effect of the temperature just before the onset of cold acclimation (pre‐acclimation) on freezing tolerance of timothy (Phleum pratense L.), perennial ryegrass (Lolium perenne L.) and red clover (Trifolium pratense L.) populations (both cultivars and breeding populations) adapted to either northern or southern parts of Norway. Using phytotron experiments, we studied whether increasing pre‐acclimation temperature delays growth cessation, affects photoacclimation and reduces freezing tolerance. Furthermore, we assessed whether these effects were related to the latitudinal adaptation of the plant material. The results showed that a rise in pre‐acclimation temperature decreased both cold acclimation capacity and photoacclimation in these species. This affected the freezing tolerance, which was reduced significantly more in northern‐adapted population of timothy and perennial ryegrass compared with southern‐adapted populations. Red clover was less affected by temperature changes than the grasses.     

Establishment, Development and Yield of Forage Legumes and Grasses as Cover Crops in Grain Corn in Eastern Canada

Interseeded cover crops can minimize soil erosion, increase organic matter and nutrient levels and the yield of subsequent crops. However, their performance is very sensitive to local conditions. Field experiments were conducted at two Quebec locations in 1993 and 1994 in order to evaluate the potential use of forage legumes and grasses as interseeds in corn in eastern Canada. Twelve forage species were evaluated. Fall rye ( Secale cereal L.), hairy vetch ( Vicia villosa Roth), a mixture of red clover ( Trifolium pratense L.) and ryegrass ( Lolium multiflorum Lam), a mixture of white clover ( Trifolium repens L.) and ryegrass, subterranean clover ( Trifolium subterraneum L.), yellow sweet clover ( Melilotus officinalis Lam.), black medic ( Medicago lupulina L.), Persian clover ( Trifolium resupinatum L.), strawberry clover ( Trifolium fragiferum L.), crimson clover ( Trifolium incarnatum L.), annual alfalfa ( Medicago saliva L.) and berseem clover ( Trifolium alexandrinum L.) were seeded at two planting dates (10 and 20 days after corn emergence). The control treatments were: hand weeding, chemical weeding and non-weeded. Early seeded forages established better and had higher biomass accumulation than the late seeded ones. In the presence of larger weed populations, the interseeded forages did not develop well due to competition with the weeds. At Macdonald crimson clover provided good soil cover while Persian clover, fall rye and alfalfa provided relatively little cover. Strawberry clover and hairy vetch did not provide early ground cover due to their late development. Forage mixtures of red or white clover and rye grass established well and achieved high populations at the end of the growing season. Fall rye provided good early ground cover but senesced by the middle of the season. The better establishment and early germination of crimson clover caused a 19% reduction in corn grain yield in 1993. In 1994 none of the cover crops caused a reduction in corn yield.     

Effects of Drought and Competition by a Ryegrass Sward on the Seedling Growth of a Range of Grassland Species

In de‐intensified grassland where an increased seedling recruitment is seen as a prerequisite to enhance plant species diversity, establishment success of introduced seeds of wildflower species is often poor. Outshading by the existing sward and dry weather spells when seedlings are at an early stage of development are major reasons for a low seedling establishment. The effects of drought and competition by an existing Lolium perenne sward on the growth of seedlings of seven wildflower species were investigated under standardized environmental conditions. Without competition (control), the relative growth rate of the wildflower seedlings varied between 103 (Tragopogon pratensis) and 184 mg g^?1 day^?1 (Leontodon autumnalis). Species differed in the extent of growth reduction by competition. Compared with the control, reduction of seedling dry weight ranged from 45 % (Trifolium pratense) up to 88 % (Daucus carota). The time range between onset of desiccation and first effects on growth varied between species, with T. pratense showing early and strong growth reduction in contrast to D. carota being least affected. It can be concluded, that those species which maintain a comparatively high growth rate supported by adapted shoot‐root allocation are most promising for establishment in grassland as they can exploit profiles of higher resource availability.     

Improved persistence of red clover (Trifolium pratense L.) increases the protein supplied by red clover/grass swards grown over four harvest years

UK livestock agriculture can significantly reduce its protein imports by increasing the amount of forage based protein grown on-farm. Forage legumes such as red clover (Trifolium pratense L.) produce high dry matter yields of quality forage but currently available varieties lack persistence, particularly under grazing. To assess the impact of red clover persistence on protein yield, diploid red clover populations selected for improved persistence were compared with a range of commercially available varieties. All populations were grown over four harvest years in mixed swards with either perennial ryegrass (Lolium perenne L.) or perennial plus hybrid ryegrass (L. boucheanum Kunth). Red clover and total sward dry matter (DM) herbage yields were measured in Years 1–4, red clover plant survival in Years 3 and 4 and herbage protein (CP) yield and concentration in Years 2 and 4. In general, red clover DM yield in year 4 (3.4 t ha⁻¹) was lower than in year 1 (13.9 t ha⁻¹) but the red clover populations differed in the extent of this decline. Differences in the persistence of the red clover populations in terms of plant survival and yield were reflected in the contribution of red clover to the total sward yield in Year 4, which ranged from 61% for the highest yielding population, AberClaret, to 11% in the lowest yielding, Vivi. Increased red clover DM yield was reflected in a greater CP yield (protein weight per unit area), which ranged from 1.6 t ha⁻¹ year⁻¹ to 2.9 t ha⁻¹ year⁻¹ in Year 2 and from 1.1 t ha⁻¹ year⁻¹ to 1.9 t ha⁻¹ year⁻¹ in Year 4. CP concentration (protein weight per unit herbage weight) of all of the red clover populations was within a range considered suitable for ruminant production. The implication of these results for the future use of red clover in sustainable grassland systems is discussed.     

Correlations between visual biomass scores and forage yield in space planted red clover (<Emphasis Type="Italic">Trifolium pratense</Emphasis> L.) breeding nurseries

Red clover (Trifolium pratense L.) forage yield remains a prime breeding target for improved variety development. In a world of decreasing forage legume breeding resources, rapidly and cheaply phenotyping plants for the highly quantitative trait of forage yield is vital. Many red clover selection programs are based on space planted nurseries. The objectives of this study were to determine: (1) the accuracy of visual forage yield scores in predicting actual forage yield; (2) the nature of the relationship between visual scores and actual measurements; and (3) The repeatability of visual scores between different evaluators. Twenty-seven halfsib families were transplanted at two locations in three replicates of six plant plots. Individual plant fresh weights and visual scores, by two evaluators, for forage yield were taken. On an individual plant basis visual forage yield scores showed an exponential relationship with actual fresh weights. Individual plant visual scores were very accurate with a pseudo-R ² of 0.79 observed for the exponential model. On an entry mean basis using a linear model, visual scores could explain 90% of the variation of actual fresh weights. Agreement among evaluators scoring the same plants was very high with coefficients of determination at 0.84 for individual plants and as high as 0.96 on an entry mean basis. This study suggests that visual scores of plants in space planted red clover breeding nurseries are basically as accurate as measuring actual yields and that plants can be consistently scored the same by different evaluators.     

Difference in Allelopathic Potential as Influenced by Root Periderm Colour of Sweet Potato (Ipomoea batatas)

Laboratory bioassay and high‐performance liquid chromatography (HPLC) analysis were conducted to determine the allelopathic potentials of aqueous or methanol extracts from three different coloured sweet potato [Ipomoea batatas L. (Lam)] cultivars by plant part. The aqueous extracts applied on filter paper significantly inhibited root growth of alfalfa (Medicago sativa L.). Aqueous leaf leachates at 40 g dry tissue l^?1 (g l^?1) from white sweet potato cultivar ‘Sinyulmi’ showed the highest inhibition against alfalfa, followed by yellow ‘Sinhwangmi’ and purple ‘Jami’. Alfalfa root growth was significantly inhibited by methanol extracts of the same plants as the concentration increased. Aqueous and methanol extracts from leaves showed the most inhibitory effect on alfalfa root growth followed by stems and roots. By means of HPLC analysis, leaf samples of sweet potato had the highest amount of phenolic compounds followed by stems and roots. Total content of these compounds was highest for leaf extracts (37.7 mg 100 g^?1), detected in EtOAc fraction, especially trans‐cinnamic acid (20.9 mg 100 g^?1). These results suggest that sweet potato plants are allelopathic and that their activities differ depending on plant part as well as root periderm colour.     

Influence of flower and flowering characteristics on seed yield in diploid and tetraploid red clover

Tetraploid red clover (Trifolium pratense L.) often produces insufficient seed to be economically interesting. Numerous studies have identified poor pollination due to long corolla tubes as its cause, but other studies disagree. Therefore, we investigated seed yield, corolla tube length and flowering traits in 244 plants from five diploid and five tetraploid cultivars during 2 years. Tetraploids produced fewer seeds, fewer flower heads and fewer seeds per head, but an equal number of flowers per head when compared to diploids. Although corolla tubes were longer in tetraploid than in diploid red clover (P < 0.001), no correlation between corolla tube length and seed yield was detected. Therefore, the corolla tube length is likely neither the main nor only cause of low seed yield in tetraploid red clover. Instead, three putative causes for low seed yield were identified: tetraploids produced less inflorescences per plant, possessed a lower degree of determinacy and had markedly less seeds per head when compared to diploids. Possible causes for such differences include lower pollen viability and higher rates of embryo abortion.     

Effect of spring fertilization on ecosystem services of organic wheat and clover relay intercrops

Nitrogen (N) deficiency and weed infestation are main factors limiting yield and yield stability in organic wheat. Organic fertilizers may be used to improve crop performance but off-farm input costs tend to limit profitability. Instead, forage legumes may be inserted into the crop rotation to improve the N balance and to control weed infestation. In opposition to simultaneous cropping, relay intercropping of legumes in organic winter wheat limits resource competition for the legume cover crop, without decreasing the performance of the associated wheat.The aim of this study is to evaluate the effect of spring organic fertilization on the performance of intercropped legumes and wheat, and on services provided by the legume cover.Two species of forage legumes (Trifolium pratense L. and Trifolium repens L.) were undersown in winter wheat (Triticum aestivum L. cv Lona) in five organic fields during two consecutive crop seasons. Organic fertilizer was composed of feather meal and applied on wheat at legume sowing. The cover crop was maintained after the wheat harvest and destroyed just before sowing maize.Spring organic nitrogen fertilization increased wheat biomass (+35%), nitrogen (+49%), grain yield (+40%) and protein content (+7%) whatever the intercropping treatment. At wheat harvest, red clover biomass was significantly higher than white clover one (1.4 vs. 0.7 t ha⁻¹). Nitrogen fertilization decreased forage legume above-ground biomass at wheat harvest, at approximately 0.5 t ha⁻¹ whatever the specie. No significant difference in forage legume biomass production was observed at cover killing. Nitrogen accumulation in legume above-ground tissues was significantly higher for white clover than for red clover. Both red and white clover species significantly decreased weed infestation at this date. Nitrogen fertilization significantly increased weed biomass whatever the intercropping treatment and decreased nitrogen accumulation in both clover species (−12%).We demonstrated that nitrogen fertilization increased yield of wheat intercropped with forage legume while the performance of legumes was decreased. Legume growth was modified by spring fertilization whatever the species.     

Differences in nitrogen fixation among field-grown red clover strains at different levels of 15N fertilization

Summary Genetic variation in fixed nitrogen (N) yield of red clover (Trifolium pratense L.) strains and cultivars was investigated using the ¹⁵N isotope dilution method under three regimes of N fertilization: 0.5, 30, and 60 N (kg N ha^–1 per cut). The yield of fixed N per cut (the mean of eight cuts over 2 production years) varied among the strains (progenies of crosses between inbred parents) from 148 to 443 mg per plant at 0.5 N, from 76 to 324 mg at 30 N, and from 69 to 300 mg at 60 N. There were significant and consistent strain differences in the percentage of clover N derived from the atmosphere (% N_dfa). However, %N_dfa was positively correlated with dry mass yield. Consequently, ranking of the strains according to fixed N yield reflected that of dry mass yield. There were only minor strain × N fertilizer interactions, suggesting that selection for enhanced N fixation can be carried out at a single rate of fertilizer N. For a selected pair of strains, the difference in yield of fixed N was confirmed in an Italian ryegrass-red clover mixture, both without and with the addition of N fertilizer (50 kg N ha^–1 per cut). Results with 7-week-old seedling plants in a growth chamber, although obtained in the presence of mineral N and with the isotope dilution method, did not adequately predict field performance. It is concluded that selection for dry matter or total N yield is likely to result in an enhanced yield of fixed N at any level of mineral N availability.Abbreviations % N_dfa percentage of clover nitrogen derived from the atmosphere by symbiotic nitrogen fixation - S₂-F₁-n progeny of pair cross between inbred parents obtained after two generations of selfing     

Genetic variability among elite red clover ( Trifolium pratense L.) parents used in Chile as revealed by RAPD markers

Red clover (Trifolium pratense L.) is one of the main forage species of temperate regions. Cultivars of red clover are heterogeneous which makes their genetic analysis difficult. We applied RAPDs (Random Amplifed Polymorphic DNA) in order to assess the genetic relationship and levels of genetic variability existing among a group of 16 elite red clover parents organised in four subsets of 4 parents each. Out of 55 primers 21 provided reproducible results. A total of 135 reliable and polymorphic RAPD bands were detected which were used to estimate genetic distances among pair-wise combinations of elite parents. Nei and Li's similarity values ranged from 0.60 to 0.77, with a mean of 0.66, which reflects a rather high genetic variability among the genotypes evaluated. Lower levels of genetic variability, as detected by polymorphic loci and mean heterogeneity values, were detected in a subset of parents selected for resistance to the stem nematode. Cluster analyses resolved the different sets of parents in a manner consistent with what is known from their breeding origins. An Analysis of Molecular Variance detected substantial levels of variation within subsets of parents. RAPDs represent a valuable source of genetic information for red clover breeding programmes. This revised version was published online in August 2006 with corrections to the Cover Date.     

The Effect of Sward Management on the Mineral Content of Winter Grazed Herbage

No information is available regarding the mineral content of autumn‐saved herbage during winter grazing under Central European conditions. Therefore, P, K, Na, Mg and Ca concentrations of autumn‐saved growths of Festuca arundinacea and Lolium perenne were analysed considering the potential influence of pre‐utilization (saved from June or July), date of winter harvest (December, January, February) and year (three winters). For all years date of winter harvest was the main source of variance, whereas date of preceding cut had no relevant effect on the mineral concentrations during winter. Already in December P [2.3 to 4.0 g kg^?1 dry matter (DM)], Na (0.3 to 3 g kg^?1 DM) and Mg (1.0 to 2.4 g kg^?1 DM) concentrations were mostly below the required levels for ruminants. The lowest values were detected in February. K levels ranged between 6.6 g kg^?1 DM in February and 23.4 g kg^?1 DM in December; on average, K concentrations decreased about 10 g kg^?1 DM with advancing winter. Ca concentrations (2.9 to 7.4 g kg^?1 DM) hardly changed during winter. Related to higher growth rates of F. arundinacea before December, P and Ca concentrations were diluted, but regarding Mg, more wintergreen F. arundinacea reached higher values than L. perenne especially at the beginning of winter.     

Morphological characteristics associated with winter survival of five growth types of tetraploid red clover

Summary The aim of this study was to correlate flowering response of tetraploid red clover (Trifolium pratense L.) in the year of seeding with winter survival and other characteristics (vigour, bloom date). In two different years about 2000 plants of two different cultivars (Hungaropoly and Tapiopoly) were established and in the fall were classified into five growth types based on rosette formation and flower production. Non-flowering types were shorter and had few petioles at 6–8 weeks of age, but showed better survival than flowering types. Hungaropoly non-flowering types were more vigorous and bloomed earlier in the second year but Tapiopoly non-flowering types were less vigorous and bloomed later. Although non-flowering in the seeding year was associated with better survival in the second year, there was adequate variation within both types for selection for improved persistence.     

Effect of inbreeding on pseudo-self-compatibility in red clover (Trifolium pratense L.)

Summary The effect of inbreeding on pseudo-self-compatibility (PSC) was investigated in I₀, I₁, and I₂ clones of red clover, Trifolium pratense L., at 40°C. PSC was found to decrease with inbreeding. Significant differences were found among I₀ clones, among I₁ clones, and among I₂ clones for PSC. Significant differences in PSC within generations were found in two of eight I₁ clones and one of five I₂ clones.A new method of inbred line maintenance is proposed which combines the desirable features of vegetative and seed maintenance of inbred lines. Superior I₁ clones with different but homozygous S genotypes and PSC values of 8 to 10% would be vegetatively increased. Clones would be isolated under high temperature field conditions and selfed seed (PSC) would produce the I₂. Selfed seed from two different I₁ clonal lines would then be mixed and sown to produce single-cross seed. Singlecross seed obtained from different clonal sources would be mixed and sown for the production of double-cross hybrid red clover.Contribution from the Kentucky Agricultural Experiment Station. This paper (72-3-125) is published with approval of the Director, Kentucky Agricultural Experiment Station, Lexington.     

Biological Activity and Quantification of Suspected Allelochemicals from Alfalfa Plant Parts

Autotoxicity restricts reseeding of alfalfa (Medicago sativa L.) after alfalfa until autotoxic chemical(s) breaks down or is dispersed into external environments. A series of aqueous extracts from leaves, stems, roots and seeds of alfalfa ‘Vernal’ were bioassayed against alfalfa seedlings of the same cultivar to determine their autotoxicity. The highest inhibition was found in the extracts from the leaves. Extracts at 40 g dry tissue l^?1 from alfalfa leaves were 15.4, 17.5 and 28.7 times more toxic to alfalfa root growth than were those from roots, stems and seeds, respectively. A high‐performance liquid chromatography (HPLC) analysis with nine standard compounds showed that the concentrations and compositions of allelopathic compounds depended on the plant parts. In leaf extracts that showed the most inhibitory effect on root growth, the highest amounts of allelochemicals were detected. Among nine phenolic compounds assayed for their phytotoxicity on root growth of alfalfa, coumarin, trans‐cinnamic acid and o‐coumaric acid at 10^?3 m were most inhibitory. The type and amount of causative allelochemicals found in alfalfa plant parts were highly correlated with the results of the bioassay, indicating that the autotoxic effects of alfalfa plant parts significantly differed.