Levan fructotransferase from Arthrobacter oxydans J17-21 catalyzes the formation of the di-D-fructose dianhydride IV from levan

A new levan fructotransferase (LFTase) isolated from Arthrobacter oxydans J17-21 was characterized for the production of difructose dianhydride IV (DFA IV). LFTase was purified to apparent homogeneity by Q-Sepharose ion exchange chromatography, Mono-Q HR 5/5 column chromatography, and gel permeation chromatography. The enzyme had an apparent molecular mass of 54000 Da. The optimum pH for the enzyme-catalyzed reaction was pH 6.5, and the optimum temperature was observed at 45 degrees C. The LFTase was activated by the presence of CaCl(2) and EDTA-2Na but inhibited strongly by MnCl(2) and CuSO(4) at 1 mM and completely by FeSO(4) and Ag(2)SO(4) at 1 mM. A bacterial levan from Zymomonas mobilis was incubated with an LFTase; final conversion yield from the levan to DFA IV was 35%. Neither inulin, levanbiose, sucrose, dextran, nor starch was hydrolyzed by LFTase. DFA IV was very stable at acidic pH and high temperature, thus indicating that DFA IV may be suitable for the food industry and related areas.     

Study of influential factors on oligosaccharide formation by fructosyltransferase activity during stachyose hydrolysis by Pectinex ultra SP-L

The influence of reaction conditions for oligosaccharide synthesis from stachyose using a commercial enzymatic preparation from Aspergillus aculeatus (Pectinex Ultra SP-L) was studied. Oligosaccharides were analyzed by gas chromatography with flame ionization detection (GC-FID) and matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS). Galactosyl-melibiose (DP(3)) was synthesized as a result of fructosidase activity, whereas fructosyl-stachyose (DP(5)) and difructosyl-stachyose (DP(6)) were formed as a consequence of the fructosyltransferase activity of Pectinex Ultra SP-L. The optimal reaction conditions for the synthesis of penta- and hexasaccharides were 60 °C, pH 5.5, 600 mg/mL stachyose, and 34 U/mL enzyme. Reaction time played an important role in oligosaccharide mixture composition constituted by 20% DP(5), 0.7% DP(6), 55% stachyose, 21% galactosyl-melibiose, and 1% monosaccharides after 1 h and 16% DP(5), 4% DP(6), 27% stachyose, 44% galactosyl-melibiose, and 2% monosaccharides after 3 h. In conclusion, stachyose could be used as a substrate for the enzymatic synthesis of new oligosaccharides that may open new opportunities in the development of future prebiotics.     

In vitro fermentation of alternansucrase raffinose-derived oligosaccharides by human gut bacteria

In this work, in vitro fermentation of alternansucrase raffinose-derived oligosaccharides, previously fractionated according to their degree of polymerization (DP; from DP4 to DP10), was carried out using small-scale pH-controlled batch cultures at 37 °C under anaerobic conditions with human feces. Bifidogenic activity of oligosaccharides with DP4-6 similar to that of lactulose was observed; however, in general, a significant growth of lactic acid bacteria Bacteroides , Atopobium cluster, and Clostridium histolyticum group was not shown during incubation. Acetic acid was the main short chain fatty acid (SCFA) produced during the fermentation process; the highest levels of this acid were shown by alternansucrase raffinose acceptor pentasaccharides at 10 h (63.11 mM) and heptasaccharides at 24 h (54.71 mM). No significant differences between the gas volume produced by the mixture of raffinose-based oligosaccharides (DP5-DP10) and inulin after 24 h of incubation were detected, whereas lower gas volume was generated by DP4 oligosaccharides. These findings indicate that novel raffinose-derived oligosaccharides (DP4-DP10) could be a new source of prebiotic carbohydrates.     

乙二醇-氯化铁预处理对棉秆酶水解效率的影响

为提高棉秆的纤维素酶水解效率,该研究以乙二醇为预处理溶剂,氯化铁为催化剂对棉秆进行预处理,实现了棉秆木质素和半纤维素的有效去除,提高了酶水解效率。以木质素和半纤维素的去除率为指标,运用正交试验方法优化乙二醇-氯化铁预处理条件。结果表明,棉秆在90%乙二醇水溶液,0.1 mol/L氯化铁,固液比1∶15,160 ℃条件下处理20 min,木质素和半纤维素去除率分别为85.7%和88.9%。相较原料,预处理后棉秆酶解率提高了7.6倍,葡萄糖产率达到100%（基质浓度5%,酶载量8.3 FPU/g,水解72 h条件下）。通过结构表征发现乙二醇-氯化铁预处理使棉秆的比表面积增大,致密结构被破坏,有效提高了棉秆的纤维素酶可及性。     

化学处理对竹笋壳润湿性的影响

竹笋壳疏水性好,润湿性差,影响自身及与其他材料的复合,该文旨在通过化学处理提高竹笋壳表面润湿性。分别采用不同浓度的HCl、Na OH溶液对竹笋壳进行不同时间的处理,测定处理前后竹笋壳内外表面的接触角,分析处理后内外表面的表面自由能,并用扫描电镜(scanning electron microscope,SEM)分析处理前后表面微观结构的变化。结果表明:HCl、Na OH对竹笋壳表面处理效果较好的分别为质量分数5%HCl处理6 h和质量分数2%Na OH处理6 h。水和酚醛胶作为接触试剂时,竹笋壳内外表面接触角的排列顺序均为:未处理组质量分数5%HCl处理6 h组质量分数5%Na OH处理6 h组。质量分数5%HCl处理6 h的竹笋壳内外表面的表面自由能分别提高了1%、77.68%,质量分数2%Na OH处理6 h的竹笋壳内外表面的表面自由能分别升高58.33%、79.17%。SEM表面微观结构分析表明质量分数5%HCl处理6 h的竹笋壳内表面可明显看到气孔,外表面微乳突数量增多;质量分数2%Na OH处理6 h的竹笋壳内表面纤维方向更加清晰,外表面微乳突数量增多。综上所述,质量分数2%Na OH处理6 h的竹笋壳内外表面润湿性改善效果最好,质量分数5%HCl处理6 h和质量分数2%Na OH处理6 h对竹笋壳外面表的润湿性改进的效果相近。     

Enantiomeric synthesis of (S)-2-methylbutanoic acid methyl ester, apple flavor, using lipases in organic solvent

Enantiomeric selective synthesis of (S)-2-methylbutanoic acid methyl ester, which is known as a major apple and strawberry flavor, was performed from racemic 2-methylbutanoic acid using lipases in organic solvent. Among 20 lipases, lipase IM 20 (immobilized lipase of Rhizomucor miehei), lipase AP (Aspergillus niger), and lipase FAP-15 (Aspergillus javanicus) exhibited higher enzymatic activities and enantioselectivities and were selected for the synthesis of (S)-2-methylbutanoic acid methyl ester. Using these enzymes, the reaction conditions such as temperature and lyophilizing pH were optimized, and kinetic parameters were determined. All of the reactions were performed in isooctane, which was identified as the best reaction media for nonaqueous systems. At 20 degrees C maximum enantiomeric excess was observed, while synthetic activity increased as the temperature increased. Only lipases lyophilized at pH 5.5, 6. 0, 6.5, and 7.0 showed synthetic activity. In this pH range, enantioselectivities were not influenced by the lyophilizing pH. The K(M,S) and K(M,R) values for ester synthetic activity of lipase were 1120 and 1240 mM, respectively. Enzyme activity was inhibited by (S)-2-methylbutanoic amide, and its K(i) was calculated as 84 mM. (S)-2-Methylbutanoic amide acted as a competitive inhibitor.     

Production of a conjugated fatty acid by Bifidobacterium breve LMC520 from α-linolenic acid: conjugated linolenic acid (CLnA)

This study was performed to characterize natural CLnA isomer production by Bifidobacterium breve LMC520 of human origin in comparison to conjugated linoleic acid (CLA) production. B. breve LMC520 was found to be highly active in terms of CLnA production, of which the major portion was identified as cis-9,trans-11,cis-15 CLnA isomer by GC-MS and NMR analysis. B. breve LMC520 was incubated for 48 h using MRS medium (containing 0.05% L-cysteine · HCl) under different environmental conditions such as atmosphere, pH, and substrate concentration. The high conversion rate of α-linolenic acid (α-LNA) to CLnA (99%) was retained up to 2 mM α-LNA, and the production was proportionally increased nearly 7-fold with 8 mM by the 6 h of incubation under anaerobic conditions at a wide range of pH values (between 5 and 9). When α-LNA was compared with linoleic acid (LA) as a substrate for isomerization by B. breve LMC520, the conversion of α-LNA was higher than that of LA. These results demonstrated that specific CLnA isomer could be produced through active bacterial conversion at an optimized condition. Because many conjugated octadecatrienoic acids in nature are shown to play many positive roles, the noble isomer found in this study has potential as a functional source.     

LIMESTONE PARTICLE SIZE AND RESIDUAL LIME CONCENTRATION AFFECT PH BUFFERING IN CONTAINER SUBSTRATES

The objective was to quantify how the concentration and particle size of unreacted “residual” limestone affected pH buffering capacity for ten commercial and nine research container substrates that varied in residual calcium carbonate equivalents (CCE) from 0.3 to 4.9 g CCE·L^?1. The nine research substrates contained 70% peat:30% perlite (by volume) with dolomitic hydrated lime at 2.1 g·L^?1, followed by incorporation of one of four particle size fractions [850 to 2000 μm (10 to 20 US mesh), 250 to 850 μm (20 to 60 US mesh), 150 to 250 μm (60 to 100 US mesh), or 75 to 150 μm (100 to 200 US mesh)] of a dolomitic carbonate limestone at 0, 1.5 or 3.0 g·L^?1. Substrate-pH buffering was quantified by measuring the pH change following either (a) mineral acid drenches without plants, or (b) a greenhouse experiment where an ammonium-based (acidic) or nitrate-based (basic) fertilizer was applied to Impatiens wallerana Hook. F. Increasing residual CCE in commercial substrates was correlated with greater pH buffering following either the hydrochloric acid (HCl) drench or impatiens growth with an ammonium-based fertilizer. Research substrates with high applied lime rate (3.0 kg·m^?3) had greater pH buffering than at 0 or 1.5 g·L^?1. At 3 g·L^?1, the intermediate limestone particle size fractions of 250 to 850 μm and 150 to 250 (20 to 60 or 60 to 100 US mesh) provided the greatest pH-buffering with impatiens. Particle fractions finer than 150 μm reacted quickly over time, whereas buffering by particles coarser than 850 μm was limited because of the excessively slow reaction rate during the experimental periods. Addition of acid from either an ammonium-based fertilizer or HCl reduced residual CCE over time. Dosage with 40 meq acid from HCl per liter of substrate or titration with HCl acid to substrate-pH of 4.5 were well-correlated with pH buffering in the greenhouse trials and may be useful laboratory protocols to compare pH buffering of substrates. With nitrate fertilizer application, residual CCE did not affect buffering against increasing pH. Residual limestone is an important substrate property that should be considered for pH management in greenhouse crop production under acidic conditions.     

Effect of water tension on ethylene production and consumption in montane and lowland soils in Austria

The trace gas ethylene affects plant growth and atmospheric chemistry and it interferes with soil restoration. In soil ethylene is simultaneously produced and consumed by different microorganisms. The effects of land use and soil moisture conditions on processes leading to an accumulation of ethylene are still unclear. We measured the rates at which montane and lowland soils from Austria produced and consumed ethylene over a range of water tensions and oxygen supply. Complete anaerobiosis (waterlogging, zero tension) favoured ethylene production, whereas ethylene degradation rates were greatest in soils at 30 kPa water tension. Soils from the lowland region of eastern Austria produced ethylene at rates of up to 12 pmol C₂H₄ g^–1 h^–1 under anaerobic conditions, and they consumed ethylene at rates reaching 231 pmol C₂H₄ g^–1 h^–1, after addition of 20 μl l^–1 ethylene. Deciduous forest soils consumed ethylene fastest. Ethylene formed rapidly and was also consumed rapidly in soils rich in humus and total nitrogen. Soils taken from the mountains both produced and consumed ethylene more rapidly than lowland soils did. Production rates reached 146 pmol C₂H₄ g^–1 h^–1 under anaerobic conditions. Spruce forest soils produced significantly more ethylene than pastures. Ethylene formation was negatively correlated with soil pH. In montane soils ethylene production was related to the availability of simple carbon sources, expressed by the amount of extractable glucose equivalents. Maximum ethylene degradation amounted to 895 pmol g^–1 h^–1. Most of the soils were net sinks for ethylene at a water tension of 30 kPa and drier.     

Enzymatic synthesis of gamma-glutamylvaline to improve the bitter taste of valine

The taste of several bitter amino acids is reduced, sourness produced, and preference increased by gamma-glutamylization. An enzymatic method for synthesizing gamma-Glu-Val involving bacterial gamma-glutamyltranspeptidase (GGT) was developed. The optimum reaction conditions for the synthesis of gamma-Glu-Val were 20 mM Gln, 300 mM Val, and 0.04 U/ml GGT, pH 10. After 3-hr incubation at 37 degrees C, 17.6 mM gamma-Glu-Val was obtained, with the yield being 88%. gamma-Glu-Val was purified on a Dowex 1 x 8 column and then identified by NMR.     

Separation of Water‐Soluble Proteins from Cereals by High‐Performance Capillary Electrophoresis (HPCE)

Most research concerning grain proteins has concentrated on the gluten storage proteins. The albumins and globulins are the water‐ and salt‐soluble proteins that contain biologically active enzymes and enzyme inhibitors. A free‐zone capillary electrophoresis method was developed to separate these proteins. Optimization included sample extraction method, capillary temperature, buffer composition, and additives. The optimal conditions for separation of these proteins was 50 μm i.d. × 27 cm (20 cm to detector) capillary at 10 kV (with a 0.17 min ramp‐up time) and 25°C. The optimum buffer was 50 mM sodium phosphate, pH 2.5 + 20% acetonitrile (v/v) (ACN) + 0.05% (w/v) hydroxypropylmethyl‐cellulose (HPMC) + 50 mM hexane sulfonic acid (HSA). Sample stability was an issue that was addressed by lyophilizing fresh extracts and redissolving in aqueous 50% ethylene glycol and 10% separation buffer. This method was successfully used in both wheat flour and whole meal samples. Comparisons were made of several wheats of different classes as well as several cereal grains. This methodology could be useful in screening cereal grains for important enzymes and their impact on end‐use quality such as food functionality, food coloration, and malting quality.     

Contents of Indigestible Fraction,Water Solubility,and Color of Pyrodextrins Made from Waxy Sorghum Starch

To investigate the effect of prethinning of starch by acid before pyrolysis on the formation of indigestible fraction (IF) in pyrodextrins, native and prethinned (50°C for 1, 4, and 24 hr) waxy sorghum starches were heated at 120–160°C with 20–60 μL of 9% HCl/g of starch. Pyrodextrin containing 14.6% IF, measured as total dietary fiber by enzymatic‐gravimetric method, was produced at 120°C with 20 μL of HCl from native waxy sorghum starch. Prethinning before pyrolysis increased IF content by 0–68%, depending on the conditions for pyrolysis, compared with that of the native starch. Reduction in the molecular size of starch by prethinning might cause greater mobility during pyroconversion reaction and thus generate higher IF contents. Increasing temperature and acid concentration during pyroconversion also increased IF content of pyrodextrins. Pyrodextrin of 44.9% IF was produced at 160°C with 60 μL of HCl from prethinned starch (50°C for 24 hr). Solubility of pyrodextrins was inversely proportional to IF content (r = ‐0.87) and had a range of 62.7–98.3%. Color of pyrodextrins became brownish with more severe pyroconversion conditions.     

Characterization of alginase and elicitor-active oligosaccharides from Gracilibacillus A7 in alleviating salt stress for Brassica campestris L

Alginase was purified from Gracilibacillus A7 and evaluated for its ability to produce elicitor-active oligosaccharides. The optimum conditions for the alginase reaction are as follows: temperature, 40 °C; pH, 8.0; alginate content, 0.3-0.7%; and the presence of Na(+) and Mg(2+) metal ions. The degree of polymerization (DP) decreased as the reaction time of the alginase progressed, achieving values of 5.4 and 3.3 after 240 and 300 min, respectively. The relative root length (RRL) of the Brassica campestris L. increased with the addition of oligosaccharides with reduced DP values. The oligosaccharides with lower DP values are effective in reducing the effect of salt stress on the activity of the superoxide dismutase (SOD) and guaiacol peroxidase (POD), and oligosaccharides with moderate DP values can reduce the increase in lipid peroxidation activities (as malondialdehyde content) induced by salt stress. These results suggest that oligosaccharides may act as osmoprotective agents during the plant germination process.     

Chemistry of Cr in some Swedish soils. 6. Native P transformation and changes in pH and cation exchange capacity in two soils incubated with potassium chromate

Abstract

Transformation of native P and changes in water pH and cation exchange capacity (CEC‐pH 7) were investigated in acid (I) and neutral (IV) soil incubated with 0, 50 and 100 mg Cr/kg for 3 months. Phosphorus was sequentially obtained as P‐resin, P‐NaHCO₃, P‐NaOH and P‐HCl, with the P‐NaHCO₃ and P‐NaOH being separated into organic and inorganic fractions. The low Cr level had little impact on the parameters. The high level increased the pH from 5.1 to 7.3 and from 6.8 to 7.5 in soils I and IV, respectively, while also significantly (P=5%) increasing CEC and decreasing P‐resin content. Subsequent to the Cr treatment, total P‐NaHCO₃ significantly declined in Soil I, but did not change markedly in Soil IV. Although total P‐NaOH was not affected by the Cr applications, its inorganic form doubled in Soil IV. Most of the total P‐NaHCO₃ and P‐NaOH was in organic form. Whereas P‐HCl was stable in Soil IV, the P nearly doubled in Soil I at the expense of P‐NaHCO₃ (r = ‐0.94**). pH was correlated with CEC (0.62*), total P‐NaHCO₃ (‐0.83**) and P‐HCl (0.76**), while CEC was correlated with P‐resin (‐0.70**), total NaHCO₃ (‐0.88**) and P‐HCl (0.94**).     

Stability of three forms of vitamin B6 to laboratory light conditions

Pyridoxine.HCl, pyridoxal.HCl, and pyridoxamine.2HCl solutions were exposed to several laboratory light treatments, and the resulting vitamin retentions were determined by the AOAC microbiological method. The 5 treatments compared were total darkness, regular laboratory light, low actinic glass protection, yellow incandescent light, and golden fluorescent light. All treatments were imposed for 8 and 15 hr, and with the vitamin solutions at both a low and a high pH. Regular laboratory light was the most destructive to the vitamins, with greater destruction at higher pH and longer exposure time in all cases. Pyridoxine retentions ranged from 97 (pH 4.5, 8 hr) to 66% (pH 7, 15 hr); pyridoxal from 97 (pH 4.5, 8 hr) to 55% (pH 6, 15 hr); and pyridoxamine from 81 (pH 4.5, 8 hr) to 47% (pH 8, 15 hr). Retentions in low actinic glassware or in clear glassware under yellow or golden fluorescent light were essentially com,lete, ranging from 94 to 106% over all treatments and all 3 forms. Results showed that either of the 2 subdued light conditions, yellow or golden fluorescent light, is suitable in vitamin B6 assays and that low actinic glassware is suitable for storing sample solutions.     

玉米秸秆液化制备生物高聚物材料的研究(英)

该研究旨在探讨低温生物质液化技术及液化产物应用的可能性。以玉米秸秆为原料在酸性、常压条件下快速液化成多羟基化合物，再以多羟基化合物为原料合成一系列的聚合材料。采用不同的有机溶剂，在稀硫酸的催化作用下，对不同的温度下生物质的液化效果进行研究。同时探讨了液化有机溶剂同生物质物料的混合比率对液化过程的影响。试验表明，碳酸乙烯酯比乙烯醇具有较高的液化率。优化试验结果表明，在较佳的液化效果下， 有机溶剂同玉米秆的混合比率为3∶1，反应温度160℃，稀硫酸浓度3%，反应时间2.5 h。液化产物经稀释、调节pH值、过滤、臭氧氧化一系列过程的处理后得到具有高活性多羟基聚合物。阐述了以多羟基聚合物制备各种生物聚合物材料如聚酯薄膜、聚胺酯泡沫和颗粒板的方法。聚酯薄膜是多羟基化合物上的羟基和多元酸上的羧基通过酯化反应形成的；聚胺酯泡沫通过多羟基化合物上的羟基和二异氰酸酯反应形成。研究表明以多羟基化合物和多元酸（酐）形成的聚酯型胶粘剂适合于制造颗粒板。     

Leaching of ethylene glycol and ethanol in subsoils

The use of the heat pumps connected to ground coils wins widespread interest in the heating of buildings, but in cases of leakage it may involve a risk of ground water pollution caused by anti-freeze mixture components. The adsorption of individual components, such as ethylene glycol, ethanol, methanol, and benzoic acid onto different soil types was studied at 6 °C by use of a batch procedure. No adsorption (or only slight) was observed for any of the test compounds onto samples of subhorizons of sandy till, clayey till, and melt water sand. Leaching experiments with undisturbed soil cores of sandy till showed that ¹⁴C-labelled ethylene glycol or ethanol closely followed the movement of water when cloride was used as a tracer. The percolation experiments were conducted during 79 to 154 day periods at 10 °C and under anaerobic conditions. Darcy velocities varied between 1 and 3 mm day^?1. About 100% of the added amounts of ¹⁴C were recovered in the effluents, although only 74 to 77% of ethylene glycol and 84 to 89% of ethanol were determined as the parent compounds by gas chromatography. Experiments on leaching of ethanol under controlled oxygen supply showed that degradation took place dependent on oxygen availability.     

Capillary electrophoretic analysis of gamma-aminobutyric acid and alanine in tea with in-capillary derivatization and fluorescence detection

The aim of this study was to investigate an in-capillary derivatization capillary electrophoresis (CE) technique that was performed to determine the concentration of gamma-aminobutyric acid (GABA) and alanine (Ala) in tea after being derivatized with o-phthaldialdehyde/2-mercaptoethanol (OPA/2-ME) to form fluorescence-labeled products. The conditions of labeled derivatization and CE separation were optimized and then applied to real sample analysis. The labeled derivatization with 20 mM OPA and 26.67 mM 2-ME (mol ratio=0.75) at pH 10 offered the most sensitive detection, and the separation with 30 mM sodium tetraborate buffer (pH 10.0) under 21 kV achieved good selectivity within 14 min. The detections were linear in the range of 0.05-5 microM with correlation coefficients (R2) of 0.9995 and 0.9964 and with detection limits of 0.004 and 0.02 microM for GABA and Ala, respectively. The recoveries were 94.22% (3.58% RSD) and 93.54% (6.46% RSD) for five determinations of GABA and Ala, respectively. This method is a fast, convenient, sensitive, and eco-friendly way to determine the GABA and Ala in tea samples from different manufacturing processes.     

Enantioseparation of imazalil residue in orange by capillary electrophoresis with 2-hydroxypropyl-beta-cyclodextrin as a chiral selector

Chiral resolution of imazalil, a fungicide, was performed by capillary electrophoresis (CE) using 2-hydroxypropyl-beta-cyclodextrin as a chiral selector. Factors affecting the chiral resolution and migration time of imazalil were studied. The optimum running conditions were found to be 5 mM ammonium dihydrogenphosphate-50 mM phosphate buffer (pH 3.0) containing 4 mM 2-hydroxypropyl-beta-cyclodextrin with an effective voltage of +25 kV at 20 degrees C using direct detection at 200 nm. Under these conditions, the resolution (Rs) of racemic imazalil was approximately 6. The extraction of imazalil from orange samples was done with acetonitrile under basic conditions. The extract was purified with a solid-phase extraction cartridge (Sep-Pak plus PS-2) and was analyzed by the above CE method. Eight orange samples were analyzed, and imazalil was detected in seven samples. In four of these seven oranges, the level of (-)-imazalil was the same as that of (+)-imazalil, but in the other three oranges, the level of (-)-imazalil was found to be lower than that of (+)-imazalil, suggesting that (-)-imazalil was degraded more quickly than (+)-imazalil in oranges.     

A Hybrid Approach for PAHs and Metals Removal from Field-Contaminated Sediment Using Activated Persulfate Oxidation Coupled with Chemical-Enhanced Washing

The aim of this study was to investigate the removal of both polycyclic aromatic hydrocarbons (PAHs) and heavy metals from field-contaminated sediments by activated persulfate oxidation. Various chemicals, including hydroxypropyl-??-cyclodextrin (HPCD), S,S-ethylenediaminedisuccinic acid (EDDS), tetrasodium pyrophosphate (Na₄P₂O₇), and hydrochloric acid (HCl), were applied individually before or after activated persulfate oxidation to enhance the co-removal of both types of pollutants. It was found that the organic removal efficiency was not significantly enhanced by increasing the concentration of HPCD from 2.5 to 5.0?mM. The removal efficiency of heavy metals was not improved even at an excess amount of EDDS after activated persulfate oxidation. However, the addition of EDDS acted as the Fe²⁺ carrier for activated persulfate oxidation. In addition, no significant enhancement of heavy metal removal was observed by increasing the concentrations of Na₄P₂O₇ and HCl from 0.01 to 0.1?M after activated persulfate oxidation. However, comparing 0.1?M HCl with 0.1?M Na₄P₂O₇, HCl was shown to be more effective in promoting the removal of organic pollutants. With further adjustments on the experimental conditions, the highest removal amount of metals and PAHs was achieved by adding 2?M of HCl with 3?days mixing, followed by Fe²⁺-activated persulfate oxidation (PS/Fe²⁺ molar ratio at 4:1) for further 6?h mixing. The removal efficiency of low and high molecular weight PAHs was about 70 and 20?%, respectively, while the removal efficiency of metals was 70, 100, 40, 65, 65, 80, and 100?% for Cr, Cu, Hg, Mn, Ni, Pb, and Zn, respectively.