Descriptive Analyses of Deficiency Symptoms in Calla Lily Plants

The objective of this work was to describe the nutrition deficiencies symptoms in calla lily (Zantedeschia aethiopica). Seedlings were maintained in complete nutrition solution and with omission of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe), boron (B), and manganese (Mn), totalizing 10 treatments. The first deficiencies symptoms appeared in plants cultivated in N omission, followed by treatments with omission of S, B, K, Fe, P, and Ca. Individual omissions of N and B prevented blooming, while S and K deficiencies resulted in production of small and greenish inflorescences with deformities. Reddish spots in leaves were observed in plants growing in solution with omission of P. Root development was affected either by omission of B or S. Death of the apical bud and formation of a “black center” in calla lily rhizome was caused by B deficiency. Nitrogen, B, and S are the most required nutrients for calla lily plants. The mineral composition of calla lily leaves is affected by nutritional deficiencies     

Induced nutrient deficiencies in Catharanthus roseus impact ajmalicine bioproduction

Plants are key sources to obtain drugs and related medical compounds. In the species Catharanthus roseus L.G. Don, ordinarily known as vinca, contains significant amounts of the alkaloid ajmalicine, which is used as an anti-hypertension drug. The aim of this research was to verify the effect of macro-nutrient deficiencies and boron over ajmalicine bioproduction in vinca roots. The experiment was performed in random blocks with eight treatments: complete, nitrogen deficient (-N), phosphorus deficient (-P), potassium deficient (-K), calcium deficient (-Ca), magnesium deficient (- Mg), sulfur deficient (-S) and boron deficient (-B), with four replicates. Seedlings were initially watered once a day with a complete solution and after 55 days after germination the treatments were started. Plants were collected 135 days after germination and evaluated in relation to root's dry matter, concentration of N, P, K, Ca, Mg, S, B and ajmalicine. Concentration of N, P, K, Ca, Mg, S and B within the root's dry matter of the vinca plants in deficient treatments were respectively of 62, 61, 93, 52, 70, 61 and 44% lower when compared with complete treatment. Potassium deficiency resulted in ajmalicine increment of 19% within roots, while deficiencies in N, P, Mg and S reduced ajmalicine concentration in 55, 33, 22 and 26% respectively when compared with complete treatment. Deficiencies in Ca and B had no significant effect in ajmalicine concentration within the plant roots. Within plants with the complete treatment, nutrient contents of ajmalicine from the roots’ dry matter were respectively of 18,5 g kg^?1 N, 1,46 g kg^?1 P, 13,5 g kg^?1 K, 4,47 g kg^?1 Ca, 1,43 g kg^?1 Mg, 1,53 g kg^?1 S, 61 mg kg^?1 B and 1,28 mg g^?1 of vitexin.     

Rootstock Effect on Nutrient Concentration of Sweet Cherry Leaves

The present investigation aimed to study the leaf mineral composition of sweet cherry trees on different rootstocks, since the literature data on uptake efficiency of different rootstocks is inconsistent. Results confirmed the different uptake efficiency of rootstocks. The efficiency of ‘GiSelA 6’ root is emphasized in uptake and supply of leaves with nitrogen (N), phosphorus (P), potassium (K), zinc (Zn), boron (B), and manganese (Mn), but trees on this rootstock tend to develop calcium (Ca), magnesium (Mg), and copper (Cu) deficiencies. The Prunus mahaleb rootstocks on calcareous sandy soil are efficient supplier of N, P, K, Ca, Mg, Fe, and Cu, but this root tends to develop Zn, B, and Mn deficiencies. Prunus avium seedling as rootstock proved to be less efficient in supply of leaves by N, P, K, Ca, and Cu. Prunus fruticosa ‘Prob’ root showed tendency in developing several leaf nutrient deficiencies. The applied fertilizer program led to low nutrient levels or even deficiency symptoms in leaves.     

Mineral Deficiency in Passiflora alata Curtis: Vitexin Bioproduction

ABSTRACT

A greenhouse experiment was performed to evaluate macronutrients and boron deficiencies on vitexin bioproduction by sweet passion fruit leaves. Sand irrigated with nutrient solution was used as substrate in a complete randomized design, with eight treatments: 1) complete, 2) nitrogen-deficient(-N), 3) phosphorus-deficient(-P), 4) potassium-deficient(-K), 5) calcium-deficient(-Ca), 6) magnesium-deficient(-Mg), 7) sulfur-deficient(-S), and 8) boron-deficient(-B). After thirty days, the fourth fully expanded leaves were harvested. Under deficiency treatments, leaf dry matter concentrations of N, P, K, Ca, Mg, S, and B were 52, 53, 62, 76, 69, 31, and 80% lower than in complete treatment, respectively. Nitrogen, P, and K deficiency increased vitexin leaf concentration by 46, 16, and 18%, although Ca and B deficiencies decreased vitexin concentration by 22 and 33%, respectively, when compared to complete treatment. Magnesium and S deficiencies had no significant effect on vitexin concentration. In complete treatment, the concentration of nutrients and vitexin, in leaf dry matter were: 43.4 g kg^{? 1} of N, 2.47 g kg^{? 1} of P, 27.4 g kg^{? 1} of K, 15.6 g kg^{? 1} of Ca, 3.8 g kg^{? 1} of Mg, 5.28 g kg^{? 1} of S, 64 mg kg^{? 1} of B, and 5.57 mg kg^{? 1} of vitexin.     

Changes in mineral nutrient concentrations and C‐N metabolism in cabbage shoots and roots following macronutrient deficiency

The responses of metabolic networks to mineral deficiency are poorly understood. Here, we conducted a detailed, broad‐scale analysis of macronutrient concentrations and metabolic changes in the shoots and roots of cabbage (Brassica rapa L. ssp. pekinensis) plants in response to N, P, K, Ca, and Mg deficiency in nutrient solution. To standardize individual macronutrient‐deficient treatments, the concentrations of the other nutrients were maintained via substitution with other ions. Individual nutrient deficiencies had various effects on the uptake and accumulation of other mineral nutrients. Phosphorus deficiency had relatively little effect on other mineral nutrient levels compared to the other treatments. Cation deficiency had little effect on N and P concentrations but had a somewhat negative effect on the uptake or concentrations of the other nutrients. Primary metabolic pathways, such as energy production and amino acid metabolism, were greatly affected by mineral nutrient deficiency. Compared to the control treatment, soluble sugar levels increased under –N conditions and decreased under –Ca and –Mg conditions. The levels of several organic acids involved in glycolysis and the TCA cycle decreased in response to –N, –P, or –K treatment. The levels of most amino acids decreased under ‐ N treatment but increased under –P, –K, –Ca, or –Mg treatment. Mineral depletion also led to the activation of alternative biochemical pathways resulting in the production of secondary metabolites such as quinate. Notable changes in metabolic pathways under macronutrient deficiency included (1) a quantitative increase in amino acid levels in response to Mg deficiency, likely because the restriction of various pathways led to an increase in protein production and (2) a marked increase in the levels of quinate, a precursor of the shikimate pathway, following cation (K, Ca, and Mg) deficiency. These findings provide new insights into metabolic changes in cabbage in response to mineral deficiency and pave the way for studying the effects of the simultaneous deficiency of more than one macronutrient on this crop.     

Foliar Symptomology and Tissue Concentrations of Nutrient‐Deficient Vegetative Strawflower Plants

Abstract

Elemental deficiencies of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, manganese, copper, zinc, or boron (N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn, or B) were induced in plants of Florabella Pink strawflower [Bracteantha bracteata (Vent.) A. A. Anderberg]. Rooted stem cuttings were planted in 4.87‐L plastic containers and fertilized with a complete modified Hoagland's solution or this solution minus the element that was to be investigated. Plants were harvested for tissue analyses as well as dry weights when initial foliar symptoms were expressed and later under advanced deficiency symptoms. Deficiency symptoms for all treatments were observed within 7 weeks. The most dramatic expression of foliar symptoms occurred with N (chlorotic lower foliage leading to necrotic margins on the mature leaves), Ca (black necrotic spots on the tips of the young leaves), S (uniform chlorosis of young leaves and recently mature leaves), B (thick, leathery, and deformed young leaves), Fe (uniform yellowish‐green chlorosis on the young leaves), and Zn (brownish‐gray necrosis on the tips of the mature leaves). At the initial stage, only Fe‐deficient plants weighed less than the control, whereas K‐, Ca‐, and Mg‐deficient plants had greater dry weights than plants receiving the complete modified Hoagland's solution (control plants). Dry weights of plants treated with solutions not containing N, P, Ca, S, Cu, or Mn were significantly lower when compared with the control plants under an advanced deficiency. Foliar‐tissue concentration data will assist plant‐tissue analysis laboratories in establishing foliar symptom standards for growers.     

Macronutrient deficiency and anatomic modifications in crambe leaves

Abstract

This study had the objective of assessing growth, deficiency symptoms and leaf anatomy of crambe plants submitted to macronutrient availability. The experimental design was the complete randomized with four replications. The first treatment consisted of cultivating crambe plants in a nutrient solution completed with N, P, K, Ca, Mg, and S. Using the diagnosis by subtraction, the other treatments consisted of the same solution with individual omission of each nutrient, totaling seven treatments. Supplement of different solutions took place two weeks after emergence. One week forward, visual symptoms of deficiency started to be evaluated. By the end of the experiment, the number of leaves, number of branches, shoot dry matter and leaf anatomic parameters were evaluated. Nutrient deficiency limited shoot dry matter in the following order: N?>?Ca?>?P>Mg?>?S?>?K. Subtracting Ca from the solution was most limiting to crambe growth once plants did not even reach reproductive stages. Individual subtractions of each macronutrient anatomically altered crambe leaves, especially omitting Ca, K, and S, which reduced tissue thickness.     

缺素培养对大豆营养生长和根系形态的影响

通过营养液栽培试验,探讨了巴西10号和本地2号两个大豆基因型在氮、磷、钾、钙、镁、硫、铁缺乏条件下的缺素症状和植株生物量变化,以及根系形态指标的改变。结果表明,缺素处理对大豆植株生长影响显著,在各种缺素条件下大豆植株生物量均明显降低,地上部与根部均表现出各种营养元素缺乏时的特有症状;缺氮、缺钾、缺钙、缺镁和缺铁处理时大豆总根长和根表面积也均显著降低。大豆对不同缺素处理的反应存在基因型差异。     

Optimum Nutrient Rate and Nutritional Constraints in Tuber Crops Growing in Ultisol of India with Special Emphasis on Tannia

Tuber crops are generally grown in marginal lands with low native soil fertility. In India, laterite soils (acidic Ultisols) are the major soils for tropical tuber crops and are poor in innate fertility. Among tropical tuber crops, some have adapted to poor soils, such as cassava, whereas others such as tannia (Xanthosoma sagittifolium L.) cannot establish well in these soils and may manifest nutritional disorders, which ultimately result in the complete devastation of the crop. Therefore, we investigated the effects from a preliminary rate trial (PRT) and nutrient-omission pot trial (NOPT) using maize as a test crop and a NOPT with tannia to determine the optimum nutrient rate and limiting nutrients, as well as nutritional problems affecting the growth and yield of tannia. Each experiment was laid out in a complete randomized design with three replications and was conducted for both garden and paddy soils. The PRT revealed that the optimum nutrient requirements for the soils were different, with garden soils requiring nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), boron (B), zinc (Zn), and molybdenum (Mo) at 200, 60, 160, 70, 60, 50, 4, 8, and 0.8 kg ha^?1, respectively, and paddy soil requiring twice these rates. The NOPT indicated that in addition to N, P, K, B, and Mo in both garden and paddy soils, Ca and Zn in paddy soils and S in garden soils were the constraining nutrients. The NOPT carried out with tannia indicated that the main nutritional problem was subsoil acidity-induced multinutrient deficiencies involving K, Ca, and Mg.     

脐橙幼苗新老叶片养分含量对大、中量元素短期缺乏的差异性响应

  【目的】  对比大、中量养分短期缺乏下脐橙新、老叶片中11种必需元素含量及变化,并分析缺素导致的营养元素间的相互影响。  【方法】  以一年生枳砧纽荷尔脐橙幼苗为试材进行了砂培试验。以完全营养液为对照 (CK),设置缺氮 (?N)、缺磷 (?P)、缺钾 (?K)、缺钙 (?Ca)和缺镁 (?Mg)处理,测定不同处理脐橙叶片（老叶和新叶）生长指标及矿质元素含量。  【结果】  所有缺素处理均导致叶片叶绿素含量降低,生物量减少,以缺氮处理最为显著。缺氮降低了叶片N、Ca、Cu、Mo含量;缺磷降低了叶片P、K、Mo含量;缺钾降低了叶片K含量;缺钙降低了叶片N、Cu、Zn、Mo含量但增加了P含量;缺镁降低了叶片Ca、Mg、Zn、Mo含量但增加了K含量。以必需矿质元素为变量分别对各处理老叶和新叶进行主成分分析,老叶中第一主成分 (PC1)明显将缺钾处理与其他处理区分开,与对照相比,缺钾老叶离子组成变化为N (?3%)、P (+1%)、K (?71%)、Ca (+11%)、Mg (+39%)、B (+16%)、Mn (+11%)、Fe (+32%)、Cu (?7%)、Zn (+14%)、Mo (?63%);新叶中PC1明显将缺氮处理与其他处理区分开,缺氮新叶离子组成变化为N (?53%)、P (+8%)、K (+7%)、Ca (?14%)、Mg (+11%)、B (+55%)、Mn (+51%)、Fe (?14%)、Cu (?57%)、Zn (+4%)、Mo (?25%)。老叶和新叶中元素含量呈正相关的元素是N-Cu、N-Ca、Mg-Mn和Cu-Mo,呈负相关的是K-Zn。  【结论】  脐橙幼苗老叶对钾的短期缺乏反应最敏感,缺钾会显著降低老叶中K和Mo含量并增加Mg和Fe含量,而新叶对氮素的短期缺乏最敏感,缺氮显著降低新叶中N、Ca、Cu和Mo含量。短期缺少P、Ca和Mg对脐橙幼苗叶片中的养分含量影响较小。     

Genotypic variation in foliar nutrient concentrations, δ13C,and chlorophyll fluorescence in relation to tree growth of radiata pine clones in a serpentine soil

This study investigated the genotypic variation in foliar nutrient concentrations, isotopic signature (δ¹³C), and chlorophyll fluorescence (F_v/F_m) and tree growth of 40 radiata pine clones grown on a New Zealand serpentine soil, and the relationships between growth and physiological traits of these clones from improved and unimproved groups. Genotypic variation in growth and physiological traits existed within (i.e., clonal) and between groups, with larger variation among clones. The clonal repeatabilities were greater for foliar nitrogen (N), calcium (Ca), magnesium (Mg), boron (B) concentrations, δ¹³C, and Ca : Mg ratio (0.35–0.64) than for growth traits (0.14–0.27) and other physiological traits (0.08–0.24). Significant phenotypic correlations were found between growth traits and foliar phosphorus (P), potassium (K), sulfur (S), iron (Fe), and K : Mg and Ca : Mg ratios and F_v/F_m (positive), and foliar Mg (negative). This study indicates that the trees on this serpentine soil generally suffered from multiple nutrient deficiencies and imbalances and the clonal variation in growth performance was more related to their capabilities of acclimation to nutrient than water stresses. Overall, the clones that absorbed more P, K, S, and Fe and less Mg from the soil grew better on this serpentine soil. For unimproved clones, the most limiting nutrients for tree growth were foliar K and Fe, while for improved clones it was foliar K.     

Diagnosis and recommendation integrated system approach for major and micronutrient diagnostic norms for apple (Malus Domestica Borkh) under varying ages and management practices of apple orchards

Various approaches for the Diagnosis and Recommendation Integrated System (DRIS) indices were employed like Beaufil's ER, Elwali and Gascho and Jones. As per the Beaufil's approach of DRIS indices, the nutrient requirements in the initial stage of the apple tree were magnesium > nitrogen > sulfur > phosphorus > copper > zinc > iron > manganese > boron > calcium > potassium (Mg > N > S > P > Cu > Zn > Fe > Mn > B > Ca > K) and in the later stage at 40–50 years, the nutrient requirements were S > Cu > Mg > Fe > P > N > Ca > Mn > K > Zn > B, thus demanding a foliar application of magnesium salt and urea which are required in high amounts in the initial stage; however in the later stage, the yield depression was not attributed to the nutrient deficiency but rather trees' genetic make-up which destabilizes the higher yield in the period of 50 years. Nutrient sufficiency ranges for apple derived from DRIS norms were 1.91–2.24, 0.18–0.26, 1.11–1.61, 1.74–1.88, 0.30–0.33 and 0.28–0.30% for nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S), respectively.     

Deficiency of N,P, K,Ca, Mg,or Fe Mineral Nutrients in Narcissus cv. “Garden Giant”

Narcissus cv. “Garden Giant” bulbs were grown in N, P, K, Ca, Mg, or Fe-deficient solutions and compared with bulbs grown in the control solution containing all these nutrients. Plants were sampled at 4 stages: (I) at planting, (II) at sprouting, (III) after flower senescence and/or after visible deficiency symptoms developed, and (IV) at lifting. Observation of visible deficiency symptoms showed that leaves of narcissus displayed chlorosis in the —N, —Mg, and —Fe treatments, while roots were most susceptible to Ca-deficient conditions. Root tips in the —Ca treatment showed brown in followed by root rot. In the —N treatment, shoot growth was markedly retarded and leaves were small and yellow. On the other hand, visible deficiency symptoms were not evident in the —P treatment except for early senescence. K deficiency symptoms were also not evident. Narcissus flowers were not affected by the mineral deficiencies and mineral contents in full-bloom flowers were not different between samples.

In the control plants, there was a large accumulation of N in the roots at sprouting and the content decreased thereafter. Large amounts of K accumulated in roots more than in any other organs. On the other hand the Ca content was high in the tunic and Ca in the scales was hardly mobilized thoughout the growth period. A large proportion of each element eventually accumulated in new inner scales whereas only a small fraction in the old outer scales. The -N, -Ca, and -Mg treatments severely depressed dry matter accumulation, unlike the -P, -K, and -Fe treatments. The -N treatment did not affect the concentration of other minerals, but the -P treatment tended to decrease N, K, Ca, and Mg concentrations. On the other hand, the -K treatment increased Ca and Mg concentrations. -Ca caused an increase in Mg concentration and -Mg raised Ca concentration. These results may be due to compensation effects of cation absorption for the maintenance of the cation/anion balance.     

Soil fertility and yield-limiting nutrients in oil palm plantations of north-eastern state Mizoram of India

A survey was conducted for assessment of soil fertility status, leaf nutrient concentration and finding yield-limiting nutrients of oil palm (Elaeis guineensis Jacq.) plantations in Mizoram state situated in the northeastern part of India. Soil pH, electrical conductivity (EC), organic carbon (OC), available potassium (K), available phosphorus (P) (Bray's-P), exchangeable calcium (Ca) (Exch. Ca) and magnesium (Mg) (Exch. Mg), available sulfur (S) (CaCl₂-S), and hot-water-soluble boron (B) (HWB) content in surface (0–20 cm depth) and subsurface (20–40 cm depth) soil layers varied widely. Diagnosis and Recommendation Integrated System (DRIS) norms were established for different nutrient expressions, and DRIS indices were computed. As per DRIS indices, the order of requirement of nutrients was found to be B > K > Mg > P > nitrogen (N). Optimum leaf nutrient ranges as per DRIS norms varied from 1.91% to 2.95%, 0.46% to 0.65%, 0.63% to 1.00%, 0.48% to 0.88%, and 9.41 to 31.0 mg kg^?1 for N, P, K, Mg, and B, respectively. On the basis of DRIS-derived optimum ranges, 32%, 9%, 27%, 12%, and 12% leaf samples had less than optimum concentration of N, P, K, Mg, and B, respectively. The optimum ranges developed could be used as a guide for routine diagnostic and advisory purpose for efficient fertilizer application.     

Diagnosis and Recommendation Integrated System for Monitoring Nutrient Status of Mango Trees in Submountainous Area of Punjab,India

Abstract

The Diagnosis and Recommendation Integrated System (DRIS) was used to identify nutrient status of mango fruit trees in Punjab, India. Standard norms established from the nutrient survey of mango fruit trees were 1.144, 0.126, 0.327, 2.587, 0.263, 0.141% for nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S), and 15, 3.5, 145, 155, and 30 mg kg^?1, respectively, for zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), and boron (B) in dry matter. On the basis of DRIS indices, 16, 15, 12, 17, and 16% of total samples collected during nutrients survey of mango trees were low in N, P, K, Ca, and Mg, respectively. For micronutrients, 19, 18, 12, 20, and 6% samples were inadequate in Zn, Cu, Fe, Mn, and B, respectively. DRIS‐derived sufficiency ranges from nutrient indexing survey were 0.92–1.37, 0.08–0.16, 0.21–0.44, 1.71–3.47, 0.15–0.37, and 0.09–0.19% for N, P, K, Ca, Mg, and S and 11–19, 1–6, 63–227, 87–223, and 16–44 mg kg^?1 for Zn, Cu, Fe, Mn, and B, respectively.     

菊科花卉常见缺素症及植株养分含量变化探讨

通过溶液培养的方法,研究了3种主要菊科花卉（金盏菊、孔雀草、万寿菊）在缺N、P、K、Ca 、Mg、S条件下的缺素症状及其生物量与营养元素变化情况。结果表明,金盏菊、万寿菊缺N,孔雀草缺K时症状最为明显;缺N对金盏菊、孔雀草、万寿菊的植株生物量影响最大;与全素（CK）相比,金盏菊和万寿菊缺K处理植株N浓度增加,3种花卉缺N、缺K处理均增加植株的P含量,但缺P处理降低植株K浓度,缺K处理增加含Ca量,缺Ca处理增加含K量和含Mg量,金盏菊缺Mg处理的含K量增加,3种花卉缺S处理植株S含量降幅不大,孔雀草和万寿菊缺N、P、K、Ca处理均可导致植株S含量增加。     

Nutritional Status,Yield, and Fruit Quality of “Encore” Mandarin Trees Grown in Two Sites of an Orchard with Different Soil Properties

Abstract

The nutrient status [annual fluctuation of leaf nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn)], yield and fruit quality [soluble solids concentration (SSC), titratable acids (TA), SSS/TA and juice content] of “Encore” mandarin trees cultivated in two sites of the same orchard were studied. The trees were grafted on Carrizo citrange rootstock and grown under identical conditions, apart from some soil properties. Soil B (site B of orchard) contained more K, Ca, Mg, and organic matter than soil A (site A of orchard). The patterns of annual variation of leaf nutrient concentrations were similar in both soils, although leaf concentrations of Ca, Mg, Mn, and Fe in soil A were significantly higher than those of soil boron (B), while leaf K concentrations were significantly lower. The mineral analyses of the leaves revealed some interesting antagonisms between K–Mg, K–Ca, and K–Mn. Manganese deficiency was especially limited in the trees grown in soil B. The average fruit yield per tree in soil A, on two-year basis, was significantly higher than this in soil B. The significantly higher water infiltration rate in soil B, in contrast to soil A, seemed to be the dominant factor responsible for the differences among the two sites in yielding and leaf mineral composition.     

钾、镁交互作用对橡胶幼苗生长及养分吸收的影响

  【目的】  我国植胶区砖红壤钾、镁缺乏现象日益突出,研究钾、镁缺乏对橡胶幼苗根系形态和养分吸收的影响,可为橡胶平衡施肥和优质高产栽培提供理论依据。  【方法】  选用‘热研7-33-97’橡胶 (Hevea brasiliensis) 幼苗为研究材料,在人工气候箱内用营养液培养。采用二因素二水平的析因试验设计,设置4个处理:对照 (CK)、缺钾 (–K)、缺镁 (–Mg) 和缺钾镁 (–K-Mg),培养3个月后,取样测定橡胶幼苗干物质量、根系构型参数、根系活力和养分含量等指标。  【结果】  1) 与CK相比,–K和–K-Mg处理显著降低了单株干物质量和根冠比,干物质量降幅分别为8.4%和27.5%,根冠比降幅分别为20.4%和26.9%,而–Mg处理对干物质量和根冠比均无显著影响;K、Mg交互作用对茎干、根和单株干物质量及根冠比均有显著影响 (P < 0.05)。2) 与CK相比,各缺素处理均显著降低了橡胶幼苗吸收根 (直径 < 2 mm) 的根长、根表面积、根体积、总根尖数及根系活力等根系构型参数,而不同程度增加了平均根粗。方差分析结果表明,K、Mg交互作用对吸收根的根长、根表面积、根体积及总根尖数有极显著影响 (P < 0.01)。3) 各处理下氮和镁、磷和钾以及钙分别在叶片、根系以及茎皮中的平均分配比例高于其他器官。各缺素处理下,地上部的养分占比呈增加趋势。4) 与CK相比,–K处理显著增加了橡胶幼苗单株氮、磷和镁的积累,–K-Mg处理则显著降低了单株氮积累,各缺素处理均显著增加了单株钙的积累;K、Mg交互作用对氮、磷、钙和镁的积累有显著或极显著影响。  【结论】  钾、镁营养显著影响橡胶幼苗对养分的吸收,缺钾、缺镁显著抑制橡胶幼苗特别是根系的生长发育,同时缺钾缺镁加重抑制效果。因此,橡胶生产上不仅要保证培养基质或土壤的矿质营养充足,还要重视钾、镁元素间平衡关系。     

Nitrogen and potassium effects on the macronutrient and micronutrient content of zoysiagrasses 1

Zoysiagrass has more potential for utilization in the Gulf Coast states. There has been minimal research on the nitrogen (N) and potassium (K) fertility response of zoysiagrass and the resulting effect they have on the macronutrient and micronutrient content. The objective of this study was to evaluate the effects of N and K fertility on the nutrient content of zoysiagrass. A study was conducted on four zoysiagrasses: Zoysiajaponica x Z. tenuifolia Willd. ex Trin. ('Emerald'); Z. japonica Steud. ('El Toro’ and ‘Meyer'); and Z. matrella. The study was a completely randomized design with 3 replications. It was a 2x2 factorial with the factors being N and K at two levels that were imposed on the four zoysiagrass cultivars. The N and K treatment combinations consisted of high (H) and low (L) rates of N and K at the following levels: N levels of 454 and 227 g N 93 m^‐2 month^‐1 and K levels of 454 and 227 g K 93 m^‐2month^‐1. The treatment combinations were (N and K): HH, HL, LH, and LL and were applied in 2 split applications monthly from July through November. All plots received two applications of a micronutrient fertilizer (June and August), were irrigated as needed, and maintained at a height of 3.8 cm weekly. Plant tissue samples were collected in September and analyzed for nitrogen (N), phosphorus (P), K, calcium (Ca), magnesium (Mg), sulfur (S), boron (B), copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) concentrations. There were significant differences for the concentrations of N, K, P, Ca, Mg, B, Cu, Fe, Mn, and Zn. The concentrations of K, Ca, and Mg were below the sufficiency range for these nutrients for all cultivars and treatments.     

Biochemical Markers and Nutrient Constraints Diagnosis in Citrus: A Perspective

Mineral deficiencies are well-established causal factor(s) for sub-optimum production in citrus. Identifying nutrient constraints based on morphological symptoms or in combination with leaf/soil analysis is often misleading, especially with reference to remediating the nutritional problems of a standing crop. The task becomes further confounded by other co-factors under the conditions favoring the occurrence of multi-nutrient deficiency. Important biochemical markers for various nutrient deficiencies include: ribulose-1,5 biphosphate carboxylase (RuBPCase), nitrate reductase, and glutamate dehydrogenase for nitrogen (N) deficiency; citrate synthetase, aconitase, phosphoenol pyruvate kinase, and glutamic oxaloacetic transminase for phosphorus (P) deficiency; diesterase, acid invertase, arginine decarboxylase, and N-carbamyl putrescine aminohydrolase for potassium (K) deficiency; pyruvate kinase and succinate dehydrogenase for calcium (Ca) deficiency; invertase for magnesium (Mg) deficiency; adolase and aconitase for iron (Fe) deficiency; catalase for manganese (Mn) deficiency (higher peroxidase activity under Mn deficiency is differentiated from Fe deficiency); carbonic anhydrase and nitrate reductase for zinc (Zn) deficiency; and phenylalanine ammonia lyase and nitrate reductase for boron (B) and molybdenum (Mo) deficiency, respectively. These markers have also shown some promise in establishing the physiological basis of tolerance of Satsuma mandarin (Citrus unshiu Marc.) against Mn and aluminum (Al) toxicities involving the Mn-oxidative pathway and complexation of calmodulin protein with Al³⁺ ions, respectively.