New cell line establishment of a cell line from embryos ofDendrolimus superans

A new cell line was established from 5-day-old embryonated eggs ofDendrolimus superans and has been designated NEAU-Ds-950821. The cell line consists of mixture of cell types, including majority of spherical shaped cells and a few of spindle shaped cells. The cell line has a population of doubling time of 52.6 h. Chromosome analysis revealed typical lepidopteran chromosomes. Isozyme characterization of Esterase showed the patterns were different from other three cell lines (Ms-927311, Xc-920730, and SF-21AE). Virus infectivity tests revealed the cell line can supportD. superans cytoplasmic polyhedrosis virus. The project is supported by National Science Foundation of China Responsible editor: Chai Ruihai     

Population Characteristics of Dendrolims Superans in Daxing'an Mountains

CollectionofdataFiveI1tlndredf1eadsofD..`IIl,L;l-I;(Ilt`crecoIlectedilltIletbrest.oraroul1dtbrestarcadtIril1gtl1epeakofap-pearancetYol11I99lloI992.TI1el1tlInberoftb111aIca11dlllaIeaduItst`ererecorded.T``/ostal1dardpIotsucrecI10seninYol1gqingandHa[1jiayuan…     

落叶松毛虫性引诱剂新型载体林间应用技术研究

采用普通船型诱捕器,对落叶松毛虫人工合成性引诱剂不同载体诱蛾效果进行了林间应用研究。结果表明:常规橡胶塞诱芯诱蛾时间多为3～5 d,之后需要更换新诱芯,诱蛾规律性不强,无法准确判断虫情发生趋势。而PVC微管型诱芯诱蛾效果明显优于前者,诱蛾期长达1个月,落叶松毛虫成虫发生趋势明显,为掌握松毛虫发生趋势提供科学依据,同时还大大节省人工及材料成本。     

3种松毛虫杂交试验

2009年，进行油松毛虫、赤松毛虫、落叶松毛虫三种松毛虫6种设计交叉杂交试验研究，杂交组分别为油♂×赤♀、油♂×落♀、油♀×赤♂、油♀×落♂、赤♂×落♀、赤♀×落♂。结果表明，各杂交组均产生了杂交后代，平均产卵量386粒，平均孵化率为89．68％。     

Trichosanthin down-regulates Notch signaling and inhibits proliferation of the nasopharyngeal carcinoma cell line CNE2 in vitro

The prognosis of nasopharyngeal carcinoma (NPC) is still poor. Trichosanthin (TCS) has abortifacient, anti-virus, immunoregulation and various anti-tumor pharmacological activities, but there are no reports about its effect on NPC and the exact mechanisms that TCS inhibits tumor are not well known. In this study, the proliferation, apoptosis and soft agar colony formation abilities of CNE2 cells were examined with various assays in vitro followed by treatment with TCS. Furthermore, the activation status of Notch signaling pathway in TCS and control cells also was examined. The results revealed that TCS could inhibit NPC cell line CNE2 in vitro, reduce clone formation ability and induce apoptosis of CNE2 cells. Down-regulation of Notch signaling may be one of the mechanisms that TCS inhibits NPC.     

Antiproliferative activity of Thai medicinal plant extracts on human breast adenocarcinoma cell line

Ethanolic extracts of selected nine Thai medicinal plants were tested for antiproliferative activity against SKBR3 human breast adenocarcinoma cell line using MTT assay. Garcinia mangostana showed the most potent activity. However, all plant extracts showed activity in potential range for further investigation on cancer cells.     

Establishment of cell suspension line of Populus tomentosa Carr

Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L-1 2,4-D. The best subculture medium was MS + 0.8 mg...     

呼和浩特地区落叶松毛虫灾害气象预报方法

根据神经网络工作原理,对内蒙古呼和浩特市落叶松毛虫灾害与主要气象因子用bp神经网络建模,在Matlab中实现,预测出呼和浩特地区落叶松毛虫发生面积值,预测结果比较理想。     

Antiproliferative effects of Ceratonia siliqua L. on mouse hepatocellular carcinoma cell line

Extracts from pods and leaves of carob (Ceratonia siliqua L.) were tested for their ability to inhibit cell proliferation of mouse hepatocellular carcinoma cell line (T1). The two extracts showed a marked alteration of T1 cell proliferation in a dose-related fashion reaching the maximal effect at 1 mg/ml. Moreover, we demonstrated that leaf and pod extracts were able to induce apoptosis in T1 cell lines after 24-h treatment mediating a direct activation of the caspase 3 pathway. HPLC analysis revealed the presence of gallic acid, (-) epigallocatechin-3-gallate and (-) epicatechin-3-gallate in pod and leaf extracts, compounds well known to exert antiproliferative effects. Their concentration reached 6.28 mg/g in carob leaves and 1.36 mg/g in carob pods extract. The discovery that carob pod and leaf extracts contained antiproliferative agents could be of practical importance in the development of functional foods and/or chemopreventive drugs.     

杀虫灯诱杀松毛虫的试验

通过在鹤岗市红旗林场设点布灯诱杀落叶松毛虫试验,结果表明:双灯诱杀效果好于单灯,晴天、无风天诱杀效果好。最佳开灯时间为20:00至4:00;采用杀虫灯诱杀落叶松毛虫防治成本为60元.hm2,比化学药剂防治成本低50%。     

Baicalein decreases side population proportion via inhibition of ABCG2 in multiple myeloma cell line RPMI 8226 in vitro

ObjectiveTo investigate the effect of baicalein on side population in human multiple myeloma cell line RPMI 8226 and the underlying molecular mechanisms in vitro and in silico.MethodsMTT assay was applied to detect the anti-proliferation effect of baicalein. The detection of side population cells is based on the Hoechst 33342 exclusion assay technique and flow cytometric analysis. Western blotting assay was used to explore the expression of ABCG2 protein. Homology modeling and molecular docking were performed with Discovery Studio 2.1.ResultsBaicalein decreased both cell viability with IC₅₀ = 168.5 μM and the proportion of SP cells in a dose-dependent manner. Correspondingly, it significantly decreased the expression level of ABCG2 protein. Baicalein also shared similar binding sites and modes with fumitremorgin C to the protein.ConclusionsBaicalein possessed novel anticancer properties, such as anti-proliferation and drug efflux inhibition in side population cells, which suggested its potential feature of targeting cancer stem cells of multiple myeloma.     

Outbreaks of Mass Reproduction of Siberian Moth in the 2nd Half of the 20th Century in Priamurye

Siberian moth Dendcrolimus superans sibiricus Tschetw. is the main important insect pest not only in Siberian coniferous taiga, but it often forms foci of mass reproduction in larch stands in the Russian Far East. This article has described outbreaks of the Siberian moth and other insect pests since 1960 till now.     

THE INFLUENCEN OF STAND STATE ON THE OCCURRENCE OF DENDROLIMUS SUPERANS (BUTLER) IN DAXING'AN MOUNTAINS

DendlxilinIIisSIIPeIWlsbrokeoutil1theforestofDaxil1g'anMountainsregio111nl99O.Tlleligl1tandheatydal11agedareaisone111iI-llollhln=Tlledan1agedegreeofD.SItPeIwhlsnoteve11lntheregion.Thestandsdal11agedbyDendIxihnIIissIIPeIunlnsenous'i11ten11ediateandligl1tdegreeaPpearedaspatchshaPedis-tribuholl.Thereaso11thatthedifferentresu1tsaboveapPearedisstandenviro11l11e11tclearly.Therefor,undertl1eco11dihol1oflargeareaweatl1er.Itwasnotclearlythatwhichfactor1sllla1ninthestandenviroIUnent.n1eoccu…     

用绿色威雷杀虫剂防治落叶松毛虫

绿色威雷是一种触破式微胶囊杀虫剂。于2001年4月在讷河市保安林场对该杀虫剂防治落叶松毛虫(Dendrolimus superans T.)进行了药效试验。在越冬幼虫上树之前,分别用150,250,350,和450倍药液喷洒落叶松树干,喷洒长度(高度)为1.0, 2.5, 5.5 m。防治结果表明,喷洒150,250,350倍药液取得较为理想的防治效果(幼虫死亡率达97%),450倍液的防治效果为70%幼虫死亡率。绿色威雷杀虫剂可以用作春季防治落叶松毛虫越冬幼虫的一种有效的杀虫剂。推荐使用350倍液和1m的喷洒长度。表1参5。     

BT油悬剂防治落叶松毛虫试验

通过分别使用8000IU·μL-1BT油悬剂用量3000、4500、和6000mL·hm-2及25%灭幼脲悬浮剂2000倍液、清水空白作对照等5个处理进行落叶松毛虫防治试验,结果表明:应用8000IU·μL-1BT油悬剂用药量为4500和6000mL·hm-2情况下,用药后14d的防治效果为96.6%和98.3%,分别比25%的灭幼脲2000倍液防治效果高2.5和4.2个百分点;用柴油对药剂稀释10倍后防治,对落叶松不产生药害。     

狼毒根部提取液对几种常见森林害虫的防治试验

利用33%的狼毒根部提取液对落叶松毛虫，分月扇舟娥进行防治的效果较好，分别达到79.31%和77.78%，而对青杨虎天牛防治效果较差，只有20.00%。     

Invasive sequence and ecological niche of main insect borers of <Emphasis Type="Italic">Larix gmelinii</Emphasis> forest in Aershan,Inner Mongolia

After the outbreak of Dendrolimus superans Buter in 2002, many insect borers quickly invaded larch （Larix gmelinii Rupr.） forests in the Aershan of Inner Mongolia. Methods involved included setting sample plots, collecting adults in iron traps and measuring areas of galleries to study the invasive sequence, their ecological niche and the extent of the different effects by the main insect borers to their hosts. The results showed that the damage of D. superans weakened L. gmelinii, first Ips subelongatus Motschulsky invaded, followed by Acanthocinus carinulatus Gebler, Monochamus urussovi Fisher and M. sutor L. After the outbreak of D. superans, the average density of longhorn beetles per L. gmelinii tree increased. The ecological niche of Ips subelongatus stretches almost from the base to the top of the trunk. The number of insects in older stands of L. gmelinii is larger than those in middle aged stands. They do not damage healthy trees of L. gmelinii. The ecological niche of A. carinulatus is higher in dead L. gmelinii trees than in weak ones. The degree of damage is directly proportional with age and depth of bark. M. urussovi mainly damages trunks below 4 m in weak trees; in dead trees they can do damage up to 6 m in height. M. sutor mainly damages trunks below 5 m in weak L. gmelinii trees; in dead trees they cause damage up to 7 m. Again, the degree of damage is directly proportional with age. None of the three species of longhorn beetles damage healthy L. gmelinii and younger trees. Among the main insect borers, the degree of damage caused by I. subelongatus is more serious than that of other insects.     

走马芹根部提取液对几种常见森林害虫的防治试验

利用33％的走马芹根部提取液对落叶松毛虫、分月扇舟蛾进行防治试验，防治效果较好，分别达到93．1％和85．12％，而对青杨虎天牛的作用较差，防治效果只有26．67％。     

噻虫胺·高效氯氟氰菊酯微囊悬浮剂防治落叶松毛虫高龄幼虫的试验效果

落叶松毛虫(Dendrolimus superans)在春季大发生时多为3~6龄,可造成严重危害。通过使用噻虫胺·高效氯氟氰菊酯微囊悬浮剂对春季高龄幼虫进行防治试验,结果表明:使用该药剂稀释1000倍液防治3~6龄幼虫见效快、效果好;虫口密度不高时可采用1800倍液喷雾施药;防治3龄以下幼虫,可稀释2500倍液喷雾防治。