美洲黑杨×青杨木材性状QTLs定位研究

利用来自 87个F2 代单株的 81 0个标记 (784个AFLP标记和 2 6个SSR标记 )构建了美洲黑杨×青杨杂种分子连锁遗传图谱。该图谱包括 1 9个主要连锁群 ,共有 36 8个标记 (35 6个AFLP标记和 1 2个SSR标记 )。框架图总图距为 3382 4cM ,平均标记间距为 9 6 9cM。利用区间作图法在LOD≥ 2 ,检测区间为 2cM条件下检测到 8个与木材性状有关的QTLs。其中与木材基本密度关联的 3个QTLs贡献率分别为 2 7 1 %、2 7 1 %、2 5 5 % ,与微纤丝角关联的QTLs贡献率为 1 8 6 % ,与纤维长度关联的QTLs贡献率为 4 8 9% ,与纤维宽关联的 3个QTLs贡献率分别为 1 7 4 %、2 7 4 %和 4 8 3%。这 8个QTLs遗传作用方式均为超显性 ,其中只有 2个与基本密度关联的QTLs来自于美洲黑杨 ,其余 6个均来自于青杨。     

美洲黑杨×青杨F_2代基本材性性状遗传变异研究

利用美洲黑杨×青杨杂交三代谱系探索了木材密度、纤维长、纤维宽、纤维长宽比和微纤丝角等性状的遗传变异规律。结果表明:基本密度与纤维长受主基因的控制,表现为"质量-数量性状",其杂种优势明显且在F2代中继续存在;纤维宽与微纤丝角表现出较为典型的微效多基因控制的数量性状遗传特点;纤维宽与基本密度、纤维宽与纤维长宽比均为显著负相关;基本密度与纤维长和微纤丝角均为弱负相关,与纤维长宽比为弱正相关;纤维长和纤维宽、纤维长和纤维长宽比显著相关;而微纤丝角与纤维长、纤维宽和纤维长宽比之间不相关。     

杂种落叶松F2代自由授粉家系纸浆材遗传变异及多性状联合选择

对17年生7个杂种落叶松自由授粉家系和3个对照子代测定林(共10个处理)的生长和材性性状进行遗传变异分析、相关分析、综合指数评价与选择.结果表明:生长与材性性状家系间、家系内均存在较大的变异,除单位面积管胞数量外,其他性状晚材的变异系数均大于早材;材积、管胞长宽比和树脂道比量变异系数较大(26.2%～85.3%).基本密度、早材微纤丝角、管胞比量变异系数较小(1.4%～8.1%).方差分析表明:处理间综纤维素含量,早、晚材微纤丝角,管胞长宽比,晚材径向直径、胞壁率、壁腔比,管胞比量差异显著;材积、管胞长、弦向直径、早材和晚材单位面积管胞数量差异极显著,家系遗传力为59.3%～92.7%,家系水平的材性改良潜力较大.材积与综纤维素含量成极显著正相关,与早材和晚材微纤丝角、弦向直径、晚材壁腔比和管胞比量成正相关,与管胞长宽比和晚材单位面积管胞数量成负相关;基本密度与材积成微弱的负相关,与综纤维素含量成显著的负相关,与管胞长宽比、早材微纤丝角、晚材单位面积管胞数量成正相关;综纤维素含量与管胞长宽比和晚材单位面积管胞数量成负相关;管胞长宽比与晚材壁腔比成极显著负相关;晚材微纤丝角与管胞比量成显著的正相关.对生长和材性进行综合评价,选出I3,I4,I93个比较理想的多性状指数方程.适合作纸浆林培育的家系为兴7×日77-2、日5×长77-3和日3×兴9.入选率30%时,各性状家系遗传增益分别为:材积34.7%,综纤维素含量7.5%,晚材壁腔比11.0%,晚材胞壁率6.2%,管胞长宽比4.5%,晚材微纤丝角3.2%,管胞比量0.3%.     

落羽杉种源木材微纤丝角和纤维形态的变异

对16个落羽杉种源的14年生人工林木材微纤丝角和木材纤维形态进行测定.结果表明:1)16个种源落羽杉木材微纤丝角存在显著差异,微纤丝角变化在29.9～35.8°之间.从径向变化看,落羽杉胸径处木材微纤丝角在髓心处最大,由髓心向外逐渐减小;从纵向变化看,微纤丝角在基干处最大,随树木高度的增加而逐渐减小.2)16个落羽杉种源木材的纤维长度和宽度均存在显著差异,纤维长度的变化在1 764.84～2 900.08 μm之间,纤维宽度的变化在28.48～51.21 μm之间,纤维长宽比的变化在42.79～82.12之间.落羽杉胸径处木材纤维形态的径向变化为纤维长度和纤维宽度从髓心到树皮逐渐增加,纵向变化规律为纤维长度和纤维宽度在树干基部较小,随树高的增加而逐渐增加.3)落羽杉木材微纤丝角与木材纤维长度呈极显著的负相关关系,与木材的纤维宽度和纤维长宽比呈一定的负相关,木材纤维长度与木材纤维宽度呈显著的正相关.4)16个落羽杉种源依据木材微纤丝角和木材纤维长度2个木材材性指标可以分为4类:第Ⅰ类,木材的微纤丝角较大,木材纤维长度中等;第Ⅱ类,微纤丝角和木材纤维长度均处于中等水平;第Ⅲ类,微纤丝角较小,木材纤维长度较长;第Ⅳ类,木材纤维长度较短,微纤丝角中等.综合考虑微纤丝角和木材纤维长度,2号和30号种源可作为纤维工业原料用材的优良种源.     

美洲黑杨×青杨F2代基本材性性状遗传变异研究

利用美洲黑杨×青杨杂交三代谱系探索了木材密度、纤维长、纤维宽、纤维长宽比和微纤丝角等性状的遗传变异规律.结果表明基本密度与纤维长受主基因的控制,表现为"质量- 数量性状",其杂种优势明显且在F     

欧美杨纤维含量构成因素的相关和通径分析

【目的】分析杨树超短轮伐林纤维含量构成因素及其贡献率,明确育种目标,为杨树超短轮伐林选育工作提供必要的依据。【方法】以5个3年生欧美杨无性系(库安托杨、凌丰1号、凌丰3号、凌丰4号和凌丰5号)为材料,利用Excel、SPSS 17.0软件对5个无性系各性状数据进行方差、标准差分析,计算变异系数和重复力,利用最小显著差异法(LSD)进行多重比较;对无性系各性状间进行相关性分析,采用回归分析方法分析并建立单株纤维含量与生长、材性性状间的关系模型;同时,对纤维含量主要构成因素进行偏相关分析和通径分析,计算贡献率,了解其作用模式。【结果】各生长性状(树高、胸径、单株材积)在5个无性系间变化幅度相对较大,无性系间存在显著差异且在无性系的遗传比重较大,而材性性状中,除单株纤维含量外,木质素、综纤维素、纤维素、基本密度、微纤丝角、纤维平均长度、纤维平均宽度、纤维长宽比、纤维粗度9个性状在无性系间变异较小;各生长性状间相关性显著,生长性状与材性性状间具有相对独立性;利用回归分析方法得到单株纤维含量与其他性状间的关系模型,经过逐步回归分析得到最终模型,结果表明单株材积、纤维素和基本密度对单株纤维含量的影响效果显著;通过偏相关分析得出单株材积、纤维素和基本密度均能很大程度地影响单株纤维含量,同时三因素间存在相互制约关系,与关系模型中表达的结果一致;通径分析表明提高单株材积对提高单株纤维含量作用最大,增加木材基本密度次之,提高纤维素作用最小,其贡献率分别为72.61%,13.55%和13.84%,与偏相关分析结果一致。【结论】欧美杨纤维含量的主要构成因素为单株材积、纤维素和基本密度;单株材积等生长性状具有很大的提升空间,且与材性性状间具有相对的独立性,应将生长性状(树高、胸径、单株材积)作为选育标准。     

马尾松木材化学组分的遗传控制及对木材育种的意义

本文利用 1 3年生马尾松子代测定林的 2 0个自由授粉家系的木材试样 ,着重研究木材化学组分的遗传学问题。研究结果表明 ,木材化学组分、基本密度和生长性状在家系间的差异都达到了极显著水平 ,并受中等至强度的遗传控制。在 6个木材化学组分中 ,灰分、戊聚糖和 1 %NaOH抽出物含量的遗传力较高 ,木质素、综纤维素和热水抽出物含量的遗传力稍低。生长性状与木材密度呈显著的负相关 ,而与木材化学组分相关性很小 ,似相互独立。遗传相关认为灰分和热水抽出物含量可分别作为综纤维素和木质素的间接选择指标。由于综纤维素和木质素含量在家系间的绝对差异较小 ,仅为 2 %～ 3% ,对选育的实际意义不大。可选择综纤维素含量高的优株进行无性繁殖加以利用     

无损检测在日本落叶松材性育种中的应用前景探讨

使用 Pilodyn和微秒计对20个日本落叶松无性系进行无损检测,研究无性系间无损检测指标的表型和遗传变异,结果表明：应力波速( SWV)的表型变异系数和遗传变异系数分别为5.09%和3.57%,低于 Pilodyn南向测定值(Ps)和北向测定值(Pn);3个无损检测指标无性系重复力的分布范围为0.34～0.48,说明它们受中等强度的遗传控制。研究无损检测指标与各材质性状间的关系,结果表明： Ps和 Pn与生长轮密度、早材密度、生长轮壁腔比和早材壁腔比呈极显著负相关; SWV与生长轮微纤丝角、早材微纤丝角和晚材微纤丝角呈极显著负相关,但与生长轮弹性模量、早材弹性模量和晚材弹性模量呈极显著正相关。多元回归分析表明,对 Ps,Pn和 SWV 贡献率排名前2位的材质性状分别为生长轮密度和早材壁腔比、生长轮密度和生长轮壁腔比以及早材微纤丝角和生长轮弹性模量。建立无损检测指标与相对应材质性状的线性回归方程,发现 Ps与生长轮密度间方程的决定系数(0.340)略小于 Pn与生长轮密度的决定系数(0.360); SWV 与早材微纤丝角、生长轮弹性模量间方程的决定系数分别为0.348和0.316。     

不同品种杨树人工林材性差异及其对单板胶合强度的影响

以转基因欧洲黑杨、北抗杨、转基因741杨和107杨为试验对象,研究了其物理力学性能、化学性能及解剖学特性,从理论上分析了4种杨木木材的材性及其对单板胶合性能的影响。结果表明:4种杨树木材纤维素、综纤维素和木质素含量差异不太显著,107杨纤维素含量略高于其他3种杨树木材;年轮宽度上,转基因欧洲黑杨、北抗杨大于转基因741杨和107杨;4种杨木木材密度存在显著差异,其中107杨木的密度最大,这主要是由于不同木材的孔隙度差异造成的。研究同时也表明,4种杨木木材中,107杨是胶合板制备中更适宜的树种。     

日本落叶松无性系微纤丝角遗传变异的研究

对10个10年生日本落叶松无性系的早材和晚材微纤丝角进行了测定,结果表明:早、晚材微纤丝角无性系间差异极显著,同一年轮内早材微纤丝角大于晚材.日本落叶松微纤丝角的径向变异规律为:在髓心处最大,以后逐渐减小.早、晚材微纤丝角与树木年轮间的变异模式(径向变异)以对数和乘幂式方程拟合效果较好,R2(R为相关系数)均在0.8以上.早、晚材微纤丝角同树高、形率、树皮厚度、主枝粗、枝干比、主枝夹角、主枝长的相关关系不显著,早、晚材微纤丝角同胸径、冠幅的相关关系达到显著水平;早材与晚材的微纤丝角也相关显著.早材和晚材的微纤丝角受中到强度遗传控制,广义遗传力分别为0.767 4、0.804 3.按照20%的选择率,早材和晚材的微纤丝角的遗传增益分别为21.82%和29.75%.     

Browsing Damage on Pine (Pinus sylvestris and P. contorta) by a migrating moose (Alces alces) Population in Winter: Relation to Habitat Composition and Road Barriers

In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads.     

Effects of Root Freezing on the Physiology and Growth of Picea glauca,Picea mariana and Pinus banksiana Seedlings Under Different Soil Moisture Regimes

The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage.     

Stand Structure in Pine,Spruce and Deciduous Forests: A Comparison Between Finnish and Russian Karelia

This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland.     

Growth and Terminal Bud Formation in Picea abies Seedlings Grown with Alternating Diurnal Temperature and Different Light Qualities

The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions.     

CAD与建筑设计的关系

本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。     

Natural Regeneration in Clear-cuts: Effects of Scarification,Slash Removal and Clear-cut Age

The effects of soil scarification (mounding), slash removal and clear-cut age on the natural regeneration in clear-cuts was evaluated using data from four sites in southern Sweden. The treatments were carried out during a good seed and establishment year for birch ( Betula pubescens Ehrh. and B. pendula Roth). Scarification had the strongest positive effect on the density of naturally regenerated seedlings, especially in birch, but also in pine ( Pinus sylvestris L.) and spruce [ Picea abies (L.) Karst]. Slash removal had a positive effect on birch density. No statistically significant effect of clear-cut age was found. In addition, no statistically significant interactions between clear-cut age and scarification or slash removal were found. The ingrowth of field vegetation was the fastest in areas that were not scarified, less rapid in areas scarified on old clear-cuts, and the slowest after scarification in fresh clear-cuts. In conclusion, it may be possible to control the density of birch during a good establishment year for birch. If birch is desired, the best combination of treatments is to remove the slash and scarify; otherwise, these treatments should be avoided.     

Risk Perception Among Non-industrial Private Forest Owners

Based on an enquiry, risk perception among non-industrial private forest owners is described in relation to climate change and forestry hazards. Of the respondents, 11% took action to remedy the effects of climate change. Out of a given set, hazards were ranked according to each respondent's experience of recent substantial financial loss to the estate and in relation to his or her willingness to make investments aimed at risk reduction. For each hazard, the respondent assessed the risk in four classes ranging from very high to negligible risk. Six hazards were considered most problematic in all three aspects: browsing damage, falling timber prices, damage by wind, spruce bark beetle, root rot and pine weevil. A majority of the respondents claimed to take action to reduce the risk associated with at least one hazard, while 35% did not know whether they did. Excluding climate change, the need for decision support was the largest in relation to damage by wind owing to a combination of perceived high risk and a high level of ignorance in relation to whether risk-reducing measures were taken.     

Interactions in decomposition and N mineralization between tropical legume residue components

In situ produced plant residues contain a mixture of different plant components of varying quality. To assess synergistic or antagonistic interactions occurring during the decomposition and mineralization of such mixtures, individual plant parts (stems, leaves, leaf litter and roots) or the mixture of stems, leaves and leaf litter of the agroforestry species pigeonpea (Cajanus cajan) or of crop residues of peanut (Arachis hypogaea) or of the weed hairy indigo (Indigofera hirsuta) were incubated in pots for 19 weeks. Periodically, remaining plant residues were sieved out (>2 mm), weighed and N content as well as soil mineral N determined. Above- and below-ground residues of peanut decomposed fastest and showed the largest N release in agreement with their high N concentration and low-acid detergent fibre (ADF) : N ratio. Hairy indigo was hypothesized to be of lower quality than pigeonpea because of its high-polyphenol content. However, it decomposed faster than pigeonpea, largely because of the higher N and lower lignin concentration of its components. Ranking of individual plant components for N mineralization resulted in the following pattern, leaves > leaf litter > roots > stems. In mixtures of the different plant components a similar species order in decomposition was obtained, e.g. peanut > hairy indigo > pigeonpea. The amount of N released from the mixture was dominated by stem material that comprised 46–61% of the mixture. The interactions in mixtures were relatively small for peanut (generally high-quality components) as well as for pigeonpea (low proportion of high-quality components, i.e. N rich leaf material). However, a positive interaction occurred during later stages of N mineralization in the mixture of hairy indigo as it had a significant proportion of N rich components and absence of highly reactive polyphenols. Thus, for plants with low to intermediate chemical quality attributes, manipulating plant composition (e.g. by varying harvest age, affecting stem and leaf proportions) will be important to obtain significant interactions during decomposition when its components are mixed.     

Pasture production and tree growth in a young pine plantation fertilized with inorganic fertilizers and milk sewage in northwestern Spain

The use of organic waste materials such as milk sewage as an organic fertilizer could have the dual advantages of organic-waste disposal and reduced dependence on inorganic fertilizers. The effects of fertilization with (1) conventional mineral fertilization, (2) milk sewage sludge at 40 kg N ha⁻¹ target rate and (3) no fertilization on pasture production and tree growth were examined in an experiment consisting of two pasture mixtures under a one-year-old Pinus radiata plantation with a density of 2500 trees ha⁻¹. The two pasture mixtures were: (1) Dactylis glomerata L. var. saborto (25 kg ha⁻¹) + Trifolium repens L. group Ladino (4 kg ha⁻¹) + Trifolium pratense L. var. Marino (1 kg ha⁻¹); (2) Lolium perenne L. var. Tove (25 kg ha⁻¹) + Trifolium repens L. group Ladino (4 kg ha⁻¹) + Trifolium pratense L. var. Marino (1 kg ha⁻¹). The experiment began in the spring of 1995 using a randomized block design with three replicates in Castro Riberas de Lea (Lugo, Galicia, north-western Spain). Plot size was 12 × 8 m², with a 1 m buffer strip between plots. Two-year data showed that fertilization with either material had a positive effect on pasture production, with no significant difference between the two fertilization treatments. Tree growth in the milk sewage sludge plot was significantly higher than in the control plots. Inorganic fertilization increased pasture production, but affected tree growth negatively. The results show that milk sewage sludge could be used as a fertilizer in silvo-pastoral systems. This revised version was published online in June 2006 with corrections to the Cover Date.