The accumulation of phytolith-occluded carbon in soils of different grasslands

Purpose

A better understanding of the role of grassland systems in producing and storing phytolith-occluded carbon (PhytOC) will provide crucial information in addressing global climate change caused by a rapid increase in the atmospheric CO₂ concentration.

Materials and methods

Soil samples of typical steppe, meadow steppe, and meadow in Inner Mongolia, China, were taken at 0–10-, 10–20-, 20–40-, and 40–60-cm depths in July and August of 2015. The soil phytoliths were isolated by heavy liquid (ZnBr₂), and the soil PhytOC was determined by the traditional potassium dichromate method.

Results and discussion

The results of our study showed that the storage of soil phytoliths was significantly higher in the meadow (33.44 ± 0.91 t ha^?1) cf. meadow steppe (26.8 ± 0.98 t ha^?1) and typical steppe (21.19 ± 4.91 t ha^?1), which were not different. The soil PhytOC storage was significantly different among grassland types, being: meadow (0.39 ± 0.01 t ha^?1) > meadow steppe (0.29 ± 0.02 t ha^?1) > typical steppe (0.23 ± 0.02 t ha^?1). PhytOC storage in typical steppe soil within the 0–60-cm soil layer is the lowest and that in meadow soils is the highest. The grassland type and the soil condition play significant roles in accumulation of phytoliths and PhytOC in different grassland soils. We suggest that the aboveground net primary productivity (ANPP) is important in soil phytolith accumulation and PhytOC content.

Conclusions

Phytolith and PhytOC storages in grassland soil are influenced by factors such as grass type, local climate and soil conditions, and management practices. Management practices to increase grass biomass production can significantly enhance phytolith C sequestration.

     

Effects of long-term exposure to enriched CO<Subscript>2</Subscript> on the nutrient-supplying capacity of a grassland soil

Altered soil nutrient cycling under future climate scenarios may affect pasture production and fertilizer management. We conducted a controlled-environment study to test the hypothesis that long-term exposure of pasture to enriched carbon dioxide (CO₂) would lower soil nutrient availability. Perennial ryegrass was grown for 9 weeks under ambient and enriched (ambient + 120 ppm) CO₂ concentrations in soil collected from an 11.5-year free air CO₂ enrichment experiment in a grazed pasture in New Zealand. Nitrogen (N) and phosphorus (P) fertilizers were applied in a full factorial design at rates of 0, 12.5, 25 or 50 kg N ha⁻¹ and 0, 17.5 or 35 kg P ha⁻¹. Compared to ambient CO₂, under enriched CO₂ without P fertilizer, total plant biomass did not respond to N fertilizer, and tissue N/P ratio was increased indicating that P was co-limiting. This limitation was alleviated with the lowest rate of P fertilizer (17.5 kg P ha⁻¹). Plant biomass in both CO₂ treatments increased with increasing N fertilizer when sufficient P was available. Greater inputs of P fertilizer may be required to prevent yield suppression under enriched CO₂ and to stimulate any response to N.     

Carbon dioxide fluxes following tillage from a mollisol in the Argentine Rolling Pampa

Carbon dioxide emission from soil plays an important role in the global carbon cycle. Short term losses of soil carbon due to tillage are of a variable magnitude. Our objective was to evaluate the effect of plowing the soil on CO₂-C emissions during summer in a coarse-loamy mixed thermic Typic Hapludoll from the Argentine Rolling Pampa. Temperature after tillage was higher in the plowed soil than under no-tillage, being higher the soil water content in the later treatment. Plowing the soil did not produce an immediately impact on soil surface CO₂-C emission, but induced an important CO₂-C flush few days later. A difference of 16 up to 25 kg C ha^–1 d^–1 in the CO₂-C emissions was observed from the second up to the fourth sampling dates after tillage. Difference in total CO₂-C emissions between the plowed soil and the no-tillage treatment was 580 kg C ha^–1, during the 40 days measurement period. This difference in CO₂-C emission was partitioned between residue decomposition and humus mineralization. Carbon mineralized from humus was 270 kg C ha^–1 higher under plow tillage than under no tillage. This figure represented an important extra loss of 0.48% of the soil organic carbon content from the 0–30 cm depth, as consequence of plowing in the warmest season of the year.     

Abiotic dissolution and biological uptake of nitrous oxide in Mediterranean woodland and pasture soil

Soil is generally regarded as a net emitter of nitrous oxide (N₂O). However, there are numerous field studies showing net uptake of N₂O from soil in different ecosystems. Consumption of N₂O may be abiotic (absorption by water; adsorption by soil matrix) and biotic (microbial reduction of N₂O). This study is the first using undisturbed soil cores to determine the capacity of soil to consume N₂O and discuss the fate of N₂O.We exposed the base of undisturbed soil cores from Mediterranean pasture and woodland soil to elevated concentrations of N₂O and sulphur hexafluoride (SF₆; as tracer gas). Headspace concentrations of N₂O and SF₆ were determined over time and consumption rates of N₂O were calculated ranging from 148.8 ± 19.8 ng N₂O min⁻¹ g⁻¹ to 163.8 ± 17.2 ng N₂O min⁻¹ g⁻¹ in woodland soil and from 117.2 ± 36.1 ng N₂O min⁻¹ g⁻¹ to 145.1 ± 19.4 ng N₂O min⁻¹ g⁻¹ in pasture soil. Absorption of N₂O by soil water contributed 17–49% of the total N₂O consumption. The remaining N₂O consumed by the cores was due to adsorbtion by the soil matrix and/or reduction by microbes.Mediterranean soil from different ecosystems with different nitrogen (N) loads has a great potential to store and consume N₂O, if exposed to an N₂O elevated atmosphere.     

Responses of soil organic matter and greenhouse gas fluxes to soil management and land use changes in a humid temperate region of southern Europe

We studied the effects of soil management and changes of land use on soils of three adjacent plots of cropland, pasture and oak (Quercus robur) forest. The pasture and the forest were established in part of the cropland, respectively, 20 and 40 yr before the study began. Soil organic matter (SOM) dynamics, water-filled pore space (WFPS), soil temperature, inorganic N and microbial C, as well as fluxes of CO₂, CH₄ and N₂O were measured in the plots over 25 months. The transformation of the cropland to mowed pasture slightly increased the soil organic and microbial C contents, whereas afforestation significantly increased these variables. The cropland and pasture soils showed low CH₄ uptake rates (<1 kg C ha⁻¹ yr⁻¹) and, coinciding with WFPS values >70%, episodes of CH₄ emission, which could be favoured by soil compaction. In the forest site, possibly because of the changes in soil structure and microbial activity, the soil always acted as a sink for CH₄ (4.7 kg C ha⁻¹ yr⁻¹). The N₂O releases at the cropland and pasture sites (2.7 and 4.8 kg N₂O-N ha⁻¹ yr⁻¹) were, respectively, 3 and 6 times higher than at the forest site (0.8 kg N₂O-N ha⁻¹ yr⁻¹). The highest N₂O emissions in the cultivated soils were related to fertilisation and slurry application, and always occurred when the WFPS >60%. These results show that the changes in soil properties as a consequence of the transformation of cropfield to intensive grassland do not imply substantial changes in SOM or in the dynamics of CH₄ and N₂O. On the contrary, afforestation resulted in increases in SOM content and CH₄ uptake, as well as decreases in N₂O emissions.     

Annual emissions of nitrous oxide and nitric oxide from a wheat–maize cropping system on a silt loam calcareous soil in the North China Plain

Nitrogen amendment followed by flooding irrigation is a general management practice for a wheat–maize rotation in the North China Plain, which may favor nitrification and denitrification. Consequently, high emissions of nitrous oxide (N₂O) and nitric oxide (NO) are hypothesized to occur. To test this hypothesis, we performed year-round field measurements of N₂O and NO fluxes from irrigated wheat–maize fields on a calcareous soil applied with all crop residues using a static, opaque chamber measuring system. To interpret the field data, laboratory experiments using intact soil cores with added carbon (glucose) and nitrogen (nitrate, ammonium) substrates were performed. Our field measurements showed that pulse emissions after fertilization and irrigation/rainfall contributed to 73% and 88% of the annual N₂O and NO emissions, respectively. Soil moisture and mineral nitrogen contents significantly affected the emissions of both gases. Annual emissions from fields fertilized at the conventional rate (600 kg N ha⁻¹ yr⁻¹) totaled 4.0 ± 0.2 and 3.0 ± 0.2 kg N ha⁻¹ yr⁻¹ for N₂O and NO, respectively, while those from unfertilized fields were much lower (0.5 ± 0.02 kg N ha⁻¹ yr⁻¹ and 0.4 ± 0.05 kg N ha⁻¹ yr⁻¹, respectively). Direct emission factors (EF_ds) of N₂O and NO for the fertilizer nitrogen were estimated to be 0.59 ± 0.04% and 0.44 ± 0.04%, respectively. By summarizing the results of our study and others, we recommended specific EF_ds (N₂O: 0.54 ± 0.09%; NO: 0.45 ± 0.04%) for estimating emissions from irrigated croplands on calcareous soils with organic carbon ranging from 5 to 16 g kg⁻¹. Nitrification dominated the processes driving the emissions of both gases following fertilization. It was evident that insufficient available carbon limited microbial denitrification and thus N₂O emission. This implicates that efforts to enhance carbon sink in calcareous soils likely increase their N₂O emissions.     

How do environmental factors and different fertilizer strategies affect soil CO2 emission and carbon sequestration in the upland soils of southern China?

Upland soils have been identified as a major CO₂ source induced by human activities, such as fertilizer applications. The aim of this study is to identify the characteristics of soil CO₂ emission and carbon balance in cropland ecosystems after continuous fertilizer applications over decades. The measurements of soil surface CO₂ fluxes throughout the years of 2009 and 2010 were carried out based on a fertilization experiment (from 1990) in a double cropping system rotated with winter wheat (Triticum aestivum L.) and maize (Zea mays L.) in upland soil in southern China. Four treatments were chosen from the experiment for this study: no-fertilizer application (SR), nitrogen–phosphorus–potassium chemical fertilizers (NPK), NPK plus pig manure (NPKM) and pig manure alone (M). Results showed that the mean value of soil CO₂ fluxes from 08:00 to 10:00 am could represent its daily mean value in summer period (June–August) and that from 09:00 am to 12:00 pm for the rest season of a year. Soil temperature and moisture combined together could explain 70–83% of variations of CO₂ emission. Annual cumulative soil CO₂ fluxes in the treatments with manure applications (8.2 ± 0.8 and 11.0 ± 1.2 t C ha⁻¹ in 2009, and 7.9 ± 0.9 and 11.1 ± 1.2 t C ha⁻¹ in 2010 in NPKM and M, respectively) were significantly higher than those in the treatments with non-manure addition (2.5 ± 0.2 and 3.4 ± 0.2 t C ha⁻¹ in 2009, and 2.1 ± 0.2 and 3.7 ± 0.3 t C ha⁻¹ in 2010 in SR and NPK, respectively). However, the treatments with manure applications represented a carbon sink in the soil (carbon output/input ratio < 1.0), which demonstrated potential for carbon sequestration.     

Greenhouse Gas Emissions following Conversion of a Reclaimed Minesoil to Bioenergy Crop Production

This study provides a comparative assessment of greenhouse gas (GHG) emissions when converting a reclaimed minesoil that was previously under meadow to miscanthus (Miscanthus  × giganteus ) and maize (Zea mays L.) land uses in Ohio, USA. Additionally, effluent from an anaerobic digester at rates of 0, 75, 150, and 225 kg N ha⁻¹ rates was also assessed for C and nutrient fertilization. Results from the study show that land use conversion to maize had the highest net release of GHG equivalent of 6·6 Mg CO_2equ ha⁻¹ y⁻¹, on average, across effluent application rates. Under miscanthus land use with no and high effluent application rates, net GHG equivalent on average was 4·3 Mg CO_2equ ha⁻¹ y⁻¹, which was larger when compared with that under the meadow land use (1·6 Mg CO_2equ ha⁻¹ y⁻¹). Miscanthus land use under medium rates of effluent application had similar net GHG equivalent (7·1 Mg CO_2equ ha⁻¹ y⁻¹) to the maize land use. The application of effluent did increase CO₂–C and N₂O–N emissions; but increases in above‐ground–below‐ground biomass production (1·6 Mg C ha⁻¹) in the meadow land use and C input from effluent retained in the soil in the miscanthus and maize land uses offset most of the effluent‐induced GHG equivalent emissions. Contribution of cumulative N₂O–N to GHG equivalent emissions in general was 11% when no effluent was applied and 22% when effluent was applied across land uses. Findings from this study show that land use changes from antecedent meadow to maize and miscanthus during the first year of establishment would result in net increase of GHG emissions. Published 2017. This article is a U.S. Government work and is in the public domain in the USA     

Effects of land management on CO2 flux and soil C stock in two Tanzanian croplands with contrasting soil texture

Evaluation of carbon dynamics is of great concern worldwide in terms of climate change and soil fertility. However, the annual CO₂ flux and the effect of land management on the carbon budget are poorly understood in Sub-Saharan Africa, owing to the relative dearth of data for in situ CO₂ fluxes. Here, we evaluated seasonal variations in CO₂ efflux rate with hourly climate data in two dry tropical croplands in Tanzania at two sites with contrasting soil textures, viz. clayey or sandy, over four consecutive crop-cultivation periods of 40 months. We then: (1) estimated the annual CO₂ flux, and (2) evaluated the effect of land management (control plot, plant residue treatment plot, fertilizer treatment plot, and plant residue and fertilizer treatment plot) on the CO₂ flux and soil carbon stock at both sites. Estimated annual CO₂ fluxes were 1.0–2.2 and 0.9–1.9 Mg C ha^?1 yr^?1 for the clayey and sandy sites, respectively. At the end of the experiment, crop cultivation had decreased the surface soil carbon stocks by 2.4 and 3.0 Mg C ha^?1 (soil depth 0–15 cm) at the clayey and sandy sites, respectively. On the other hand, plant residue application (7.5 Mg C ha^?1 yr^?1) significantly increased the surface soil carbon stocks, i.e., 3.5–3.8 and 1.7–2.1 Mg C ha^?1 (soil depth 0–15 cm) at the clayey and sandy sites, respectively, while it also increased the annual CO₂ fluxes substantially, i.e., 2.5–4.0 and 2.4–3.4 Mg C ha^?1 yr^?1 for the clayey and sandy soils, respectively. Our results indicate that these dry tropical croplands at least may act as a carbon sink, though the efficiency of carbon accumulation was substantially lower in sandy soil (6.8–8.4%) compared to clayey soil (14.0–15.2%), possibly owing to higher carbon loss by leaching and macro-faunal activity.     

Assessing long term effects of compost fertilization on soil fertility and nitrogen mineralization rate

Background

Fertilization with organic waste compost can close the nutrient cycles between urban and rural environments. However, its effect on yield and soil fertility must be investigated.

Aim

This study investigated the long-term effect of compost on soil nutrient and potentially toxic elements (PTEs) concentration, nutrient budgets, and nitrogen (N) mineralization and efficiency.

Methods

After 21 years of annual compost application (100/400 kg N ha^–1 year^–1 [100BC/400BC]) alone and combined with mineral fertilization, soil was analyzed for pH, organic carbon (SOC), nutrient (total N and P, N_min, extractable CAL-P, CAL-K, and Mg), and PTE (Cu, Ni, Zn) concentrations. Yields were recorded and nutrient/PTE budgets and apparent net mineralization (ANM, only 2019) were calculated.

Results

N efficiency was the highest in maize and for mineral fertilization. Compost application led to lower N efficiencies, but increased ANM, SOC, pH, and soil N, and surpluses of N, P, and all PTEs. Higher PTE concentrations were only found in 400BC for Cu. Nutrient budgets correlated with soil nutrient concentration. A surplus of 16.1 kg P ha^–1 year^–1 and 19.5 kg K ha^–1 year^–1 resulted in 1 mg kg^–1 increase in CAL-P and CAL-K over 21 years.

Conclusion

Compost application supplies nutrients to crops with a minor risk of soil-accumulation of PTEs. However, the nutrient stoichiometry provided by compost does not match crop offtakes causing imbalances. Synchronization of compost N mineralization and plant N demand does not match and limits the yield effect. In winter wheat only 65–70% of N mineralization occurred during the growth period.     

Greenhouse gas emissions from Silphium perfoliatum and silage maize cropping on Stagnosols

Background

The sustainability of bioenergy is strongly affected by direct field-derived greenhouse gas (GHG) emissions and indirect emissions form land-use change. Marginal land in low mountain ranges is suitable for feedstock production due to small impact on indirect land-use change. However, these sites are vulnerable to high N₂O emissions because of their fine soil texture and hydrology.

Aims

The perennial cup plant (Silphium perfoliatum L.) might outperform silage maize (Zea mays L.) on cold, wet low mountain ranges sites regarding yield and ecosystem services. The aim of this study was to assess whether the cultivation of cup plant also provides GHG mitigation potential compared to the cultivation of maize.

Methods

A t-year field experiment was conducted in a low mountain range region in western Germany to compare area and yield-scaled GHG emissions from cup plant and maize fields. GHG emissions were quantified using the closed chamber method.

Results

Cup plant fields emitted an average of 3.6 ± 4.3 kg N₂O-N ha^–1 year^–1 (–85%) less than maize fields. This corresponded to 74.0 ± 94.1 g CO_2-eq kWh^–1 (–78%) less emissions per produced electrical power. However, cup plant had a significantly lower productivity per hectare (–34%) and per unit of applied nitrogen (–32%) than maize.

Conclusion

Cup plant as a feedstock reduces direct field-derived GHG emissions compared to maize but, due to lower yields cup plant, likely increases emissions associated with land-use changes. Therefore, the increased sustainability of bioenergy from biogas by replacing maize with cup plant is heavily dependent on the performance of maize at these sites and on the ecosystem services of cup plant in addition to GHG savings.     

Soil carbon dioxide emission from intensively cultivated black soil in Northeast China: nitrogen fertilization effect

Purpose

The aim of this study was to understand the effect of nitrogen fertilization on soil respiration and native soil organic carbon (SOC) decomposition and to identify the key factor affecting soil respiration in a cultivated black soil.

Materials and methods

A field experiment was conducted at the Harbin State Key Agroecological Experimental Station, China. The study consisted of four treatments: unplanted and N-unfertilized soil (U0), unplanted soil treated with 225?kg?N?ha^?1 (UN), maize planted and N-unfertilized soil (P0), and planted soil fertilized with 225?kg?N?ha^?1 (PN). Soil CO₂ and N₂O fluxes were measured using the static closed chamber method.

Results and discussion

Cumulative CO₂ emissions during the maize growing season with the U0, UN, P0, and PN treatments were 1.29, 1.04, 2.30 and 2.27?Mg?C?ha^?1, respectively, indicating that N fertilization significantly reduced the decomposition of native SOC. However, no marked effect on soil respiration in planted soil was observed because the increase of rhizosphere respiration caused by N addition was counteracted by the reduction of native SOC decomposition. Soil CO₂ fluxes were significantly affected by soil temperature but not by soil moisture. The temperature sensitivity (Q ₁₀) of soil respiration was 2.16?C2.47 for unplanted soil but increased to 3.16?C3.44 in planted soil. N addition reduced the Q ₁₀ of native SOC decomposition possibly due to low labile organic C but increased the Q ₁₀ of soil respiration due to the stimulation of maize growth. The estimated annual CO₂ emission in N-fertilized soil was 1.28?Mg?C?ha^?1 and was replenished by the residual stubble, roots, and exudates. In contrast, the lost C (1.53?Mg?C?ha^?1) in N-unfertilized soil was not completely supplemented by maize residues, resulting in a reduction of SOC. Although N fertilization significantly increased N₂O emissions, the global warming potential of N₂O and CO₂ emissions in N-fertilized soil was significantly lower than in N-unfertilized soil.

Conclusions

The stimulatory or inhibitory effect of N fertilization on soil respiration and basal respiration may depend on labile organic C concentration in soil. The inhibitory effect of N fertilization on native SOC decomposition was mainly associated with low labile organic C in tested black soil. N application could reduce the global warming potential of CO₂ and N₂O emissions in black soil.     

Nitrate leaching loss under annual and perennial pastures with and without lime on a duplex (texture contrast) soil in humid southeastern Australia

Mineral N accumulates in autumn under pastures in southeastern Australia and is at risk of leaching as nitrate during winter. Nitrate leaching loss and soil mineral N concentrations were measured under pastures grazed by sheep on a duplex (texture contrast) soil in southern New South Wales from 1994 to 1996. Legume (Trifolium subterraneum)‐based pastures contained either annual grass (Lolium rigidum) or perennial grasses (Phalaris aquatica and Dactylis glomerata), and had a control (soil pH 4.1 in 0.01 m CaCl₂) or lime treatment (pH 5.5). One of the four replicates was monitored for surface runoff and subsurface flow (the top of the B horizon), and solution NO₃^– concentrations. The soil contained more mineral N in autumn (64–133 kg N ha^?1 to 120 cm) than in spring (51–96 kg N ha^?1), with NO₃^– comprising 70–77%. No NO₃^– leached in 1994 (475 mm rainfall). In 1995 (697 mm rainfall) and 1996 (666 mm rainfall), the solution at 20 cm depth and subsurface flow contained 20–50 mg N l^?1 as NO₃^– initially but < 1 mg N l^?1 by spring. Nitrate‐N concentrations at 120 cm ranged between 2 and 22 mg N l^?1 during winter. Losses of NO₃^– were small in surface runoff (0–2 kg N ha^?1 year^?1). In 1995, 9–19 kg N ha^?1 was lost in subsurface flow. Deep drainage losses were 3–12 kg N ha^?1 in 1995 and 4–10 kg N ha^?1 in 1996, with the most loss occurring under limed annual pasture. Averaged over 3 years, N losses were 9 and 15 kg N ha^?1 year^?1 under control and limed annual pastures, respectively, and 6 and 8 kg N ha^?1 year^?1 under control and limed perennial pastures. Nitrate losses in the wet year of 1995 were 22, 33, 13 and 19 kg N ha^?1 under the four respective pastures. The increased loss of N caused by liming was of a similar amount to the decreased N loss by maintaining perennial pasture as distinct from an annual pasture.     

Glomalin-related soil protein responses to elevated CO2 and nitrogen addition in a subtropical forest: Potential consequences for soil carbon accumulation

According to the economy theory, plants should preferentially allocate photosynthate to acquire below-ground resources under elevated atmospheric carbon dioxide (eCO₂) but decrease below-ground C allocation when nitrogen (N) is sufficient for plant growth. Arbuscular mycorrhizae (AM) represent a critical mechanism of below-ground nutrient acquisition for plants. The dynamics of arbuscular mycorrhizal fungi (AMF) could therefore reflect the response of plant C allocation under eCO₂ and N addition. We examined the responses of glomalin-related soil protein (GRSP) to eCO₂ (approximately 700 μmol mol⁻¹ CO₂) and/or N addition (100 kg N ha⁻¹ yr⁻¹ as NH₄NO₃) in a modeled subtropical forest to better understand its potential influence on soil C storage. We hypothesized that GRSP would increase under eCO₂ and decrease under N addition. Furthermore, the positive effects of eCO₂ on GRSP would be offset by extra N addition, and GRSP would remain unchanged under combined eCO₂ and N addition. Our results showed that the mean concentrations of easily extractable GRSP (EE-GRSP) and total GRSP (T-GRSP) were 0.35 ± 0.05 and 0.72 ± 0.13 mg C cm⁻³, respectively, which accounted for 2.76 ± 0.53% and 5.67 ± 0.92% of soil organic carbon (SOC) in the 0–10 cm soil layer. Elevated CO₂ significantly increased T-GRSP by 35.02% but decreased EE-GRSP by 5.09% in the top 10 cm soil layer. The opposite responses of T-GRSP and EE-GRSP to eCO₂ might result from an unchanged photosynthate investment to AMF with possible changes in their decomposition rates. The effect of N on GRSP was contrary to our hypothesis, i.e., there was a 1.72%–48.49% increase in T-GRSP and a slightly increase in EE-GRSP. Both EE-GRSP and T-GRSP concentrations increased under the combination of eCO₂ and N addition, which was inconsistent with our hypothesis. The significant increase of EE-GRSP under the combination of eCO₂ and N addition was partly caused by more rapid plant growth and reduced microbial diversity, and the marginal increase of T-GRSP indicated that the interaction between eCO₂ and N addition offset their independent effects. In addition, the relatively higher accumulation ratios of GRSP (22.6 ± 13.6%) compared with SOC (15.9 ± 9.4%) indicated that more rapid GRSP deposition in the soil might accelerate SOC accumulation under eCO₂ and N addition. Our results will improve the understanding of the functioning of GRSP in soil C sequestration under global environmental change scenarios.     

CO2 uptake by a soil microcosm

Sequestration of CO₂ via biological sinks is a matter of great scientific importance due to the potential lowering of atmospheric CO₂. In this study, a custom built incubation chamber was used to cultivate a soil microbial community to instigate chemoautotrophy of a temperate soil. Real-time atmospheric CO₂ concentrations were monitored and estimations of total CO₂ uptake were made. After careful background flux corrections, 4.52 ± 0.05 g CO₂ kg⁻¹ dry soil was sequestered from the chamber atmosphere over 40 h. Using isotopically labelled ¹³CO₂ and GCMS–IRMS, labelled fatty acids were identified after only a short incubation, hence confirming CO₂ sequestration for soil. The results of this in vivo study provide the ground work for future studies intending to mimic the in situ environment by providing a reliable method for investigating CO₂ uptake by soil microorganisms.     

For how long does the quality and quantity of residues in the soil affect the carbon compartments and CO<Subscript>2</Subscript>-C emissions?

Purpose

The mineralization/immobilization of nutrients from the crop residues is correlated with the quality of the plant material and carbon compartments in the recalcitrant and labile soil fractions. The objective of this study was to correlate the quality and quantity of crop residues incubated in the soil with carbon compartments and CO₂-C emission, using multivariate analysis.

Materials and methods

The experiment was conducted in factorial 4?+?2?+?5 with three replicates, referring to three types of residues (control, sugarcane, Brachiaria, and soybean), and two contributions of the crop residues in constant rate, CR (10 Mg ha^?1 residue), and agronomic rate, AR (20, 8, and 5 Mg ha^?1 residue, respectively, for sugarcane, soybean, and Brachiaria), evaluated five times (1, 3, 6, 12, and 48 days after incubation). At each time, we determined the CO₂-C emission, nitrogen and organic carbon in the soil, and the residues. In addition, the microbial biomass and water-soluble, labile, and humic substance carbons fractionated into fulvic acids, humic acids and humin were quantified.

Results and discussion

Higher CO₂-C emissions occurred in the soil with added residue ranging from 0.5 to 1.1 g CO₂-C m^?2 h^?1 in the first 6 days of incubation, and there was a positive correlation with the less labile organic soil fractions as well as residue type. In the final period, after 12 days of soil incubation, there was a higher relation of CO₂-C emission with carbon humin. The sugarcane and soybean residue (20 Mg ha^?1) promoted higher CO₂-C emission and the reduction of carbon residue. The addition of residue contributed to an 82.32 % increase in the emission of CO₂-C, being more significant in the residue with higher nitrogen availability.

Conclusions

This study shows that the quality and quantity of residue added to soil affects the carbon sequestration and CO₂-C emission. In the first 6 days of incubation, there was a higher CO₂-C emission ratio which correlates with the less stable soil carbon compartments as well as residue. In the final period of incubation, there is no effect of quality and quantity of residue added to soil on the CO₂-C emission.

     

Spatial variability and biophysicochemical controls on N<Subscript>2</Subscript>O emissions from differently tilled arable soils

Nitrous oxide (N₂O) emissions, soil microbial community structure, bulk density, total pore volume, total C and N, aggregate mean weight diameter and stability index were determined in arable soils under three different types of tillage: reduced tillage (RT), no tillage (NT) and conventional tillage (CT). Thirty intact soil cores, each in a 25 × 25-m² grid, were collected to a depth of 10 cm at the seedling stage of winter wheat in February 2008 from Maulde (50°3′ N, 3°43′ W), Belgium. Two additional soil samples adjacent to each soil core were taken to measure the spatial variance in biotic and physicochemical conditions. The microbial community structure was evaluated by means of phospholipid fatty acids analysis. Soil cores were amended with 15 kg NO₃⁻-N ha⁻¹, 15 kg NH₄⁺-N ha⁻¹ and 30 kg ha⁻¹ urea-N ha⁻¹ and then brought to 65% water-filled pore space and incubated for 21 days at 15°C, with regular monitoring of N₂O emissions. The N₂O fluxes showed a log-normal distribution with mean coefficients of variance (CV) of 122%, 78% and 90% in RT, NT and CT, respectively, indicating a high spatial variation. However, this variability of N₂O emissions did not show plot scale spatial dependence. The N₂O emissions from RT were higher (p < 0.01) than from CT and NT. Multivariate analysis of soil properties showed that PC1 of principal component analysis had highest loadings for aggregate mean weight diameter, total C and fungi/bacteria ratio. Stepwise multiple regression based on soil properties explained 72% (p < 0.01) of the variance of N₂O emissions. Spatial distributions of soil properties controlling N₂O emissions were different in three different tillages with CV ranked as RT > CT > NT.     

Determining nitrogen fate by hydrological pathways and impact on carbonate weathering in an agricultural karst watershed

Identifying the nitrogen (N) fate is complicated and a great challenge in karst watersheds because of the co-existence of natural pools and anthropogenic sources. The objective of the study was to use stable isotopic composition of dual-isotope (δ¹⁵N_Nitrate and δ¹⁸O_Nitrate) and LOADEST model approaches to trace N sources, pathways in karst watershed. The study was conducted in the Houzhai watershed, which is a typical agricultural karst watershed from July 2016 to August 2018, to reveal the N fate and the coupled carbon(C)–N processes occurring in the riverine-watershed with agricultural activities. We found that the wet deposition of total nitrogen (TN) flux was 33.50 kg hm⁻²·a⁻¹ and dissolved nitrogen (DN) flux was 21.66 kg hm⁻²·a⁻¹. The DN runoff loss was 2.10 × 10⁵ kg·a⁻¹ and the loss of DN during the wet season accounted for 95.4% over a year. In the wet season, NO₃⁻-N daily efflux was 977.62 ± 516.66 kg ha⁻¹·day⁻¹and 248.77 ± 57.83 kg ha⁻¹·day⁻¹ in the dry season. The NH₄⁺-N efflux was 29.17 ± 10.50 kg ha⁻¹·day⁻¹ and 4.42 ± 3.07 kg ha⁻¹·day⁻¹ in the wet and dry seasons, respectively. The main form output load of N was NO₃⁻-N which was more than 30 times as much as NH₄⁺-N output loss. The NO₃⁻-N caused by rainfall contributed 11.82%–53.61% to the export load. Nitrate from soil contributed over 94% of the N to Houzhai river caused by N leaching. In addition, manure and farmland soil were the main sources of groundwater in the Houzhai watersheds, the contribution rates were 25.9% and 22.5%. The chemical N fertilizers affected carbonate weathering strongly, and the HCO₃⁻ flux caused by nitrification due to N fertilizers application in soil accounted for 23.5% of the entire watershed. This study suggested that carbonate weathering may be influenced by nitrogen nitrification in the karst watershed.     

Effects of Fertilizers on Yield,Sustainability, and Soil Fertility under Rainfed Pigeon pea + Rice System in Subhumid Oxisol Soils

A long-term field experiment was conducted at the research farm of the All-India Coordinated Research Project for Dryland Agriculture, Phulbani, Orissa, India, from 2001 to 2006 to identify the best integrated nutrient-use treatments for ensuring greater productivity, profitability, sustainability, and improved soil quality in pigeon pea + rice (two rows of pigeon pea followed by five rows of rice alternately) intercropping system. In all, nine treatments, eight comprising integrated nutrient-use practices, chemical fertilizer (CF), farmyard manure (FYM), and green leaf manure (GLM) to supply nitrogen (N) at 45 kg N ha^–1 and one farmer's practice equivalent to 25 kg N ha^–1 (FYM 5 t ha^–1), were tested on a long-term basis. Results of the study revealed that 20 kg N ha^–1 (FYM) + 25 kg N (CF) gave maximum mean rice grain yield of 1.52 t ha^–1, followed by 20 kg N (GLM) + 25 kg N (urea) with grain yield of 1.51 t ha^–1. In the case of pigeon pea, 30 kg N (FYM) +15 kg N (urea) gave maximum pigeon pea grain yield of 0.94 t ha^–1, which was 34% greater than the sole application of chemical fertilizer. Pigeon pea grain yield tended to increase with increasing proportion of organic N in FYM + CF or GLM + CF combinations. Application of 20 kg N (FYM) + 25 kg N (urea) recorded maximum mean rice equivalent yield of 3.59 t ha^–1 and sustainability yield index of 59%. While studying profitability, application of 20 kg N (FYM) + 25 kg N (CF) gave maximum net returns of US$168.94 ha^–1. Impact of treatments on soil quality as assessed in terms of relative soil quality indices (RSQI) increased with increasing proportion of organic sources of N. Using an innovative and new approach, an index of integrated productivity–sustainability–profitability–soil quality performance index (I _P,S,Pr,SQ) was computed to make a precise evaluation of the treatments. Based on this index, the order of performance of the treatments was T₆ [20 N (FYM) + 25 N (CF)] (7.7) > T₇ [30 N (FYM) + 15 N (CF) (6.9)] > T₃ [20 N (GL) + 25 N (CF)] (6.8) > T₅ [10 N (FYM) + 35 N (CF) (6.6)] > T₉ [GL] (6.5) > T₈ [CF] (6.2) > T₄ [30 N (GL) + 15 N (CF)] (6.0) > T₂ [10 N (GL) + 35 N (CF)] (5.7) > T₁ [FYM at 5 t ha^–1] (4.1). Thus, the results and the methodology adopted in this study using long-term data would be very useful to researchers, farmers, land managers, and other stakeholders not only in India but also across the world under similar climatic and edaphic situations.     

Soil organic carbon and nitrogen in a Mollisol in central Ohio as affected by tillage and land use

Minimum tillage practices are known for increasing soil organic carbon (SOC). However, not all environmental situations may manifest this potential change. The SOC and N stocks were assessed on a Mollisol in central Ohio in an 8-year-old tillage experiment as well as under two relatively undisturbed land uses; a secondary forest and a pasture on the same soil type. Cropped systems had 51±4 (equiv. mass) Mg ha⁻¹ lower SOC and lower 3.5±0.3 (equiv. mass) Mg ha⁻¹ N in the top 30 cm soil layer than under forest. Being a secondary forest, the loss in SOC and N stocks by cultivation may have been even more than these reported herein. No differences among systems were detected below this depth. The SOC stock in the pasture treatment was 29±3 Mg ha⁻¹ greater in the top 10 cm layer than in cultivated soils, but was similar to those under forest and no-till (NT). Among tillage practices (plow, chisel and NT) only the 0–5 cm soil layer under NT exhibited higher SOC and N concentrations. An analysis of the literature of NT effect on SOC stocks, using meta-analysis, suggested that NT would have an overall positive effect on SOC sequestration rate but with a greater variability of what was previously reported. The average sequestration rate of NT was 330 kg SOC ha⁻¹ year⁻¹ with a 95% confidence interval ranging from 47 to 620 kg SOC ha⁻¹ year⁻¹. There was no effect of soil texture or crop rotation on the SOC sequestration rate that could explain this variability. The conversion factor for SOC stock changes from plow to NT was equal to 1.04. This suggests that the complex mechanisms and pathways of SOC accrual warrant a cautious approach when generalizing the beneficial changes of NT on SOC stocks.