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本次试验对18株细菌进行了毒性试验,其中鸡肠源细菌8株,分别为蜡状芽孢杆菌、2株枯草芽孢杆菌、短乳杆菌、戊糖乳杆菌、2株植物乳球菌和棉籽糖乳球菌;猪肠源细菌10株,分别为蜡状芽孢杆菌、枯草芽孢杆菌、植物乳杆菌、短乳杆菌、玉米乳杆菌、乳酸乳球菌乳酸亚种、植物乳球菌、棉籽糖乳球菌、棉籽糖肠球菌和粪肠球菌。通过用这些细菌对小白鼠进行为期30d的正常量和加倍量的饲喂,实验组小白鼠均未出现中毒现象,试验结束时,通过对小白鼠的解剖,实验组小白鼠的肝脏和肾脏与对照组小白鼠一样,未出现任何异常变化。结果表明:本次试验所选用的18株细菌均较安全,可作为动物微生态制剂的菌种应用。 相似文献
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对从黑龙江省部分奶牛场患乳房炎奶牛乳汁中分离的10株疑似链球菌进行了培养特性、菌落形态、染色特性、生化试验等研究,确定5株为无乳链球菌,3株为停乳链球菌,2株为乳房链球菌。溶血试验结果表明,10株链球菌中有8株出现溶血现象,其中无乳链球菌有3株呈β溶血,2株呈α溶血;停乳链球菌均呈α溶血;乳房链球菌为y溶血。动物致病性试验结果表明,停乳链球菌BHI培养液腹腔接种小白鼠0.6ml,18-24后致死率达100%;接种无乳链球菌的小白鼠均有不同程度的发病,但未见死亡;乳房链球菌对小白鼠无致病性.药敏试验结果表明、链球菌对临床上常用的青、链霉素及磺胺类药物均已产生不同程度的耐药。而红霉素、喹诺酮类药物则表现出较好的抑菌效果。 相似文献
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为研究猪丹毒丝菌CVCC43005株作为生产用菌种的最高扩繁代次以及新型佐剂对该菌种免疫原性的影响,在对猪丹毒丝菌CVCC43005株形态、生化特性、培养特性、纯粹性、血清学特性、真空度、剩余水分含量、毒力及免疫原性等研究的基础上,建立了猪丹毒丝菌CVCC43005株种子批。将CVCC43005株(1986年冻干)用培养基进行了20次传代(F1~F20),并对其中的F5、F10、F15、F20代进行冻干并抽检测定。结果显示:抽检的各代次菌种均纯粹,形态、生化特性、培养特性均符合猪丹毒丝菌的特性,血清型为2型,真空度检查均呈现白色、粉色或紫色辉光,剩余水分含量均<3.0%,9~10CFU活菌皮下注射体重18~22g小白鼠,均于7日内5/5死亡。将抽检的各代次菌种制成铝胶佐剂灭活疫苗,免疫16~18g小白鼠后均能产生10/10保护。以F5代菌株制备菌液,甲醛灭活后,分别用铝胶佐剂、矿物质白油佐剂、洛阳赛威公司的水溶性佐剂、水性复合免疫佐剂以及法国赛比克公司的MONTANIDETMIMS1313VGNST、GEL01PR6种佐剂制备灭活疫苗,经无菌及安全检查合格后进行16~18g小白鼠免疫效果对比实验,最终确定猪丹毒丝菌CVCC43005株基础种子代数为F1~F10代,生产用菌种的最高扩繁代次宜控制在5代以内,法国赛比克公司的GEL01PR佐剂为猪丹毒灭活疫苗的最佳佐剂。该菌种作为生产用菌种最高扩繁代次的确定及疫苗佐剂的筛选,为修订菌种检定依据奠定了基础。 相似文献
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用从临床型奶牛乳房炎病乳分离的10株大肠杆菌对小白鼠和鸡进行了人工感染发病试验,结果表明,牛源性大肠杆菌对小鼠和鸡均有一定的致病性,但小白鼠比鸡更敏感,随着攻菌量的加大发病及死亡率亦增加。 相似文献
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从广西不同猪场有神经症状的病猪脑分离到12株典型链球菌.采用链球菌乳胶凝集标准诊断试剂盒和ID 32 STREP链球菌生化试剂盒对分离株进行分群、生化特性鉴定,以及小白鼠的致病性试验、药敏试验等研究.结果表明:分离株有1株为C群,9株为D群,1株为D群但与G群有交叉反应,1株为未知群;生化反应特性不完全一致,有5株的生化特性差异性较大;12株菌均呈现β溶血;小白鼠对大部分分离株不敏感;分离株对氟苯尼考、阿莫西林、青霉素G敏感,对庆大霉素、氟派酸、卡那霉素完全耐药. 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2%的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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