Nitrogen studies in Lolium perenne grown for seed. IV. Response of amenity types and influence of a growth regulator

In field trials in 1979–81, perennial ryegrass cultivars Royal and Majestic (amenity) and Morenne (agricultural) produced maximum seed yields at levels of applied N ranging from 40 kg ha⁻¹ to 160 kg ha⁻¹. Available soil N levels were estimated at 55 kg ha⁻¹; hence maximum seed yields were obtained at total nitrogen levels of 95–135 kg ha⁻¹ in Royal, 95–215 kg ha⁻¹ in Morenne, and 175–215 kg ha⁻¹ in Majestic.
Applied N at rates above 120 kg ha⁻¹ either reduced or did not significantly increase seed yield, decreased seed numbers per unit area and decreased spikelets per tiller and seeds per spikelet.
The use of a growth regulator increased seed yields by preventing lodging, but did so irrespective of nitrogen application rate. It is suggested that failure to increase seed yield at high N rates is a result not of poor pollination because of lodging, but seed abortion as a consequence of competition for assimilate supply by secondary vegetative tillers.     

The preharvest use of glyphosate in the ryegrass seed crop

Preharvest application of 1 and 2 litres ha^-1 IPA glyphosate (glyphosate) to a seed crop of S24 perennial ryegrass with moisture concentrations of around 400 and 340 g kg^-1 (40 and 34% moisture content w/w) failed to significantly lower seed and straw moisture concentration at harvest and consequently did not affect combine rate. Addition of extra surfactants and ammonium sulphate to glyphosate did not significantly increase the rate of desiccation.
The quality of harvested seed was impaired at both rates and application times. Germination was significantly lowered through the production of abnormal seedlings. The germination of seed harvested in the previous year from glyphosatetreated plots decreased with storage. Seed vigour, germination rate and field emergence were also significantly decreased as a result of glyphosate application. The effect of glyphosate on seed quality precludes its preharvest use as a desiccant in the ryegrass seed crop.
Harvesting difficulties due to the production of secondary vegetative tillers may be overcome by swathing rather than direct combining.     

Productivity of mixtures of Italian ryegrass and red clover

Two red clover ( Trifolium pratense ) cultivars, Red Head (tetraploid) and Kuhn (diploid), were sown at a seed rate of 13 kg ha⁻¹ either alone or in mixture with Italian ryegrass ( Lolium multiflorum ) cv. RvP sown at seed rates of 10, 15, 20 or 30 kg ha⁻¹. RvP was also sown alone at a seed rate of 30 kg ha⁻¹ and received nil or 300 kg ha⁻¹ fertilizer a⁻¹ fertilizer N. All plots were established using the barley cultivar Midas sown at a seed rate of 100 kg ha⁻¹ as a nurse crop.
Neither clover cultivar nor ryegrass seed rate significantly influenced either dry matter harvested or botanical composition over the 3 harvest years. On average over all years the grass-clover mixtures produced 75% of the yield of the N-fertilized RvP, 125% of the clover monocultures and 225% of the unfertilized RvP. The red clover contribution to the total dry matter harvested of the mixtures averaged 45–60%. The dry matter concentrations of the mixtures were considerably higher than those of the pure clover stands. In the third year yields were markedly reduced in comparison with those in the first and second years.
It was concluded that Italian ryegrass can be a suitable companion grass for red clover. Its superior yielding capacity over other grasses such as perennial ryegrass or timothy under a conservation management can be coupled to advantage with red clover to give a sward which Is essentially stable, at least over a 2- to 3-year cropping period, although giving slightly reduced yields in the third year. Italian ryegrass-red clover mixtures, without the use of fertilizer N, can produce high DM yields of good quality herbage.     

Barley/pea mixtures as cover crops for grass re-seeds

Substantial annual fluctuations in the performance of arable silage mixtures, comprising Minerva forage pea and Goldmarker barley, were observed from ten annual sowings between 1977 and 1986, Total dry matter (DM) yield varied between 2·5 and 11·5 t ha⁻¹. Dry matter content also ranged widely from 10·3% to 20·0% and there was a large variation in the proportion of peas in the DM (0·44 to 0·90). Almost 80% of this yield variation was due to the fluctuation in pea performance and over 60% of all yield variation was related to the variation in sunshine hours and air temperature.
In 1983 and 1984 differences in seed rate of between 20 and 140 kg ha⁻¹ of Minerva and 80 and 160 kg ha⁻¹ of Goldmarker influenced the forage composition at harvest, with the pea component capable of outgrowing and suppressing the other species when sown at over 80 kg ha⁻¹. Very high yields of peas were associated with increasing stress on the undersown grass re-seed and were considered undesirable.
It was concluded that sowing rates of between 120 and 160 kg ha⁻¹ for barley and a maximum of 60 kg ha⁻¹ for peas would, in most years, provide the best compromise between attaining good arable silage yields and avoiding excessive dangers of damage of undersown grass re-seeds.     

The utilization of N fertilizer, applied to perennial ryegrass/white clover pasture growing on a humus iron podzol in N.E. Scotland

Applications of N fertilizer (0.40, 80, 120 and 240 kg N ha⁻¹ year⁻¹ in dressings of 40 or 80 kg N ha⁻¹) were made to a perennial ryegrass/white clover ( Lolium perenne L. Trifolium repens L.) pasture growing on a humus iron podzol reclaimed from heather ( Calluna vulgaris L.) moor in 1982 and 1983. Where no N was applied, estimates of Ni fixation and mineralization were almost equal, being approximately 50 kg N ha⁻¹ year⁻¹ from each source. Apparent efficiencies of fertilizer use were generally low; for each dressing they ranged between –0·7 and 25·5 kg dry matter (DM)kgN⁻¹. Also, responses to N fertilizer were affected by previous dressings. The net N recovery in harvested herbage from application of 120 kg N ha⁻¹ year⁻¹ was 30 kg N ha⁻¹ year⁻¹.     

Reseeding in the Falkland Islands: seed rate and fertilizers for establishment

Five experiments were carried out to measure the effects of seed rate and fertilizers on the establishment and early growth of reseeded grass mixtures in the Falkland Islands.
Experiments 1 and 2 compared seed rates within the range 7·5-35kg ha⁻¹. Experiment 3 assessed the effects of three levels of each of nitrogen (N), P₂O₅, K₂O and CaCO₃ applied as seedbed fertilizers. Experiments 4 and 5 were similar in intent to Experiment 3, but treatments consisted of either nil or a very heavy application of the same fertilizer elements. In all experiments, the grass seed mixtures were sown in late summer after rotavation and burning of Cortaderia pilosa dominant indigenous vegetation on peaty soils. Percentage ground cover was estimated in the following spring and dry matter production through the first year was determined by sample harvests.
Initially, heavier seed rates resulted in higher percentage ground cover of sown species, but dry matter production over the season following sowing showed a significant increase only between the 25 and 35 kg ha⁻¹ rates in Experiment 2. The modest levels of seedbed fertilizers applied in Experiment 3 had no significant effect on either percentage ground cover of sown species or dry matter production. Even at the higher levels used in Experiments 4 and 5, only N consistently increased dry matter yields, although a significant positive response was also obtained from CaCO₃ in the final harvests.
The implications of the results to farmers in the Falkland Islands who may be contemplating reseeding are discussed.     

Effect of fertilizer nitrogen rate and timing on herbage production and nitrogen use efficiency for first-cut silage

Eight field-plot experiments were carried out on established grassland swards between 1984 and 1988 to examine the effects of date and rate of application of calcium ammonium nitrate (CAN) on herbage dry matter (DM) yield and apparent efficiency of nitrogen (N) use at first-cut silage.
CAN application significantly increased ( P <0 ·001) the mean yields of herbage and N uptakes by herbage in all experiments. Herbage yields were similar ( P > 0·05) with N rates of 100 kg ha⁻¹, 125 kg ha⁻¹ or 150 kg ha⁻¹ in five experiments but in the other three there were increases above 100 kg ha⁻¹. Date of N application had a significant effect on DM yield in three experiments; this effect was inconsistent for both single and split dressings. Lower production was associated with reduced uptake of N, a trend that primarily reflected lower DM yields and not wide herbage N content variation.
It is concluded that selection of the date on which to apply fertilizer N in early spring to obtain optimum herbage yields at first-cut silage often required little precision. The use of fertilizer N rates >100 kg ha⁻¹ should be questioned where there are likely to be appreciable quantities of available N derived from non-fertilizer sources.     

The impact of low numbers of leatherjackets on grass yield

Experiments were carried out on seven sites in Fermanagh to investigate the yield loss caused by leatherjackets in grassland. Populations on the sites ranged from 65,000 to 865,000 ha⁻¹ and significant damage was recorded from all sites except the least populated. Greater yield increases were obtained by controlling leatherjackets in September when compared with control in March. Regression models were fitted and it was found that 125,000 leatherjackets ha⁻¹ in March caused a yield loss of 50 kg herbage dry matter (DM) ha⁻¹ by mid-May. Based on these figures the average yield loss to leatherjackets in Northern Ireland, from 1965 to 1982, was 208 kg DM ha⁻¹. Controlling leatherjackets in September, rather than March, increased the potential avoidable yield loss by a factor of 2.72. The average yield loss at first silage cut is therefore 566 kg DM ha⁻¹. On average, 100,000 ha grassland in Northern Ireland may be suffering an annual loss of 1t herbage DM ha⁻¹.     

The productivity and response to inorganic fertilizers of species-rich wetland hay meadows on the Somerset Moors: the effect of nitrogen, phosphorus and potassium on herbage production

The effects of fertilizer nitrogen (N), phosphorus (P) and potassium (K) on herbage production were investigated in herb-rich hay meadows in Somerset, UK. Swards were cut after 1 July each year, followed by one or two aftermath cuts. Dry-matter (DM) yield at cutting, metabolizable energy (ME) production and recovery of N, P and K were measured over 4 years.
Total annual DM production increased from 4·7t ha⁻¹ without fertilizers to 10·5t ha⁻¹ with 200 kg N, 75 kg P and 200 kg K ha⁻¹ per year, and ME output from 38·8 GJ ha⁻¹ to 92·5 GJ ha⁻¹. Applying moderate replacement rates of P and K without N increased annual DM and N yields by 43% and 36% respectively, but N response was modest unless high rates of P and K were used. Annual ME output and recovery of N, P and K were all significantly increased by taking an additional, earlier cut for silage, even though DM yield was unaffected.
The results suggest that potential output of these meadows is similar to that of a wide range of less diverse permanent pastures. Data from this and a concurrent experiment will help to estimate the financial implications of fertilizer and cutting date restrictions within Sites of Special Scientific Interest and the wider Environmentally Sensitive Areas.     

The utilized metabolizable energy output of grassland on hill farms in Northern Ireland

Forty hill farms in Northern Ireland were surveyed to obtain information on stocking rates and output and to identify factors affecting output. The mean farm area of 194 ha was composed of 155 ha hill land and 39 ha inbye land with a mean stocking rate of 0–7 cow-equivalents ha⁻¹.
On the average farm most of the grassland (88%) was over 20 years old. The preferred species content of the swards averaged 22% over the whole farm and 42% in cut swards. This latter result may be attributed to the relatively high level of fertilizer N applied to these swards (131 kg ha⁻¹) compared to the mean application rate over the whole farm of only 29 kg N ha⁻¹ and 93 kg ha⁻¹ applied to the inbye land.
Output data calculated as utilized metabolizable energy (UME) per unit area for the whole farm and separately for hill and inbye components showed that although only 21% of the farm area was inbye land almost half the annual total metabolizable energy requirements of stock had to be met by grazing inbye. The capacity of herbage to meet stock energy demands was much lower on the hill with supplementary feed having to be provided over the winter.
Calculated output from inbye land was 39 GJ ha⁻¹ compared to 7 GJ ha⁻¹ from the hill and 15 GJ ha⁻¹ from the whole farm. UME output of the whole farm was negatively correlated with farm size (r=−0 55) and positively correlated with stocking rate (r = 0 89), applied N level (r = 0 63)     

Herbage yield and animal production from grassland on three commercial dairy farms in South-East England

Yields of grass were measured on three dairy farms in 1981. Farm 1, on free-draining loamy soil over chalk, produced 10.3 t DM ha⁻¹ using 336 kg N ha⁻¹. Farm 2, on poorly-drained loam over Oxford clay, produced 12.8 t DM ha⁻¹ using 354 kg N ha⁻¹. Farm 3, on loam over clay with some free-draining sandy loam, produced 9.5 t DM ha⁻¹ with 169 kg N ha⁻¹. Allowing for differences in soil nitrogen and summer rainfall these yields were quite close to expectations from experimental results, despite considerable poaching damage to some swards.
Animal production records were kept and all livestock were weighed every 6 months, so as to calculate the utilized metabolizable energy (UME) output from grassland on each farm. These were 73, 62 and 59 GJ ha⁻¹ on farms 1, 2 and 3 respectively.
The UME output, expressed as a proportion of the yield of grass (converted to ME), was taken as the 'efficiency of utilization' of grass. This was 70% on farm 1,44% on farm 2 and 58% on farm 3. Results are presented separately for the summer and winter 6 months. Utilization by grazing appeared to be markedly reduced under very wet soil conditions. Major losses occurred in the conservation of grass.
The results begin to establish a valuable base of data from commercial farms.     

Grazing cycle length and silage supplementation: effects on the performance of lactating ewes with twin lambs

Masham ewes, stocked at 20 ewes ha⁻¹, reared twins at pasture in 1979 and 1980 and were rotationally grazed around six paddocks. There were three lengths of grazing cycle (18, 30 or 42 d) and the ewes at pasture were either offered silage ad libitum (S) for 8 weeks of lactation or they were not supplemented with silage (N). By feeding silage, S ewes and lambs moved into greater herbage masses (3735 u. 3390 kg organic matter (OM) ha⁻¹), but there was little effect on net herbage accumulation (10.5 v. 10.0 t OM ha⁻¹) and herbage intakes per ewe were similar (2.31 v . 2.27 kg OM d⁻¹) over the 7-month grazing season. During the period of silage supplementation, total intake was 19% higher for S than N ewes, lamb growth rates were slightly higher (280 v . 273 g d⁻¹) and the ewes lost less weight (−78 v −96 g d⁻¹).
For 18-, 30- and 42-d grazing cycles respectively the herbage masses before grazing were 2825, 3620 and 4240 kg OM ha⁻¹; net herbage accumulations were 9.8, 10.4 and 10.6 t OM ha⁻¹; mean daily herbage intakes by each unit (ewe+lambs) were 2.22, 2.35 and 2.30 kg OM and lamb growth rates from birth to weaning at 20 weeks were 205, 204 and 204 g d⁻¹.
Frequent grazing of relatively light herbage masses or infrequent defoliation of heavier herbage masses made little difference to sward or animal performance. It is concluded that, at this stocking rate, different grazing cycles of 18, 30 or 42 d do not affect the performance of lactating ewes.     

Slot-seeding investigations

Red clover cv. Hungaropoly was slot-seeded into a perennial ryegrass-dominant sward in April 1979. Glyphosate and paraquat were applied separately as bandsprays each at two doses and at two band widths. Control plots were either slot-seeded without a herbicide bandspray or received ±150 kg N ha⁻¹ a⁻¹. Red clover establishment was assessed and amounts of dry matter (DM) and total N accumulated were measured at two harvests in 1979 and three harvests in 1980. Bandspraying increased seedling vigour and development and resulted in the eventual replacement of 1 t grass DM ha⁻¹ by an equivalent amount of red clover. Of the treatment variables investigated, bandspray width had the greatest influence on red clover establishment and productivity. The slot-seeded area, meaned for all treatments, produced a total of 6.40 and 13.16 t DM ha⁻¹ in 1979 and 1980. This was estimated to be equivalent to the all-grass sward receiving 112 kg N ha⁻¹ a⁻¹ during the second year of the experiment or 238 kg N ha⁻¹ over the 2 years when measured in terms of N yield. Slot-seeding overcomes several of the problems associated with conventional establishment of red clover.     

The effects of stocking rate and nitrogen fertilizer on a perennial ryegrass-white clover sward

The effects of stocking rate and N fertilizer on a mixed sward of perennial ryegrass ( Lolium perenne) and white clover ( Trifolium repens ) set-stocked with sheep were examined. Sward production and composition, and sheep production were studied.
Increasing the stocking rate over the range 25–55 yearling sheep ha⁻¹ reduced herbage accumulation by about 40%, whether or not N fertilizer was applied. Increasing the stocking rate increased the density of ryegrass tillers, but reduced the density of clover stolons and the clover content of the swards. Applications of N fertilizer (200 kg N ha⁻¹ a⁻¹) increased herbage accumulation by about 20% but substantially reduced the clover content.
Liveweight gain per animal and per unit area were greater at the lower stocking rates where the clover content and nutritive value of the diet were greatest. Wool growth per unit area was greater at the higher stocking rates. Applications of 1M fertilizer increased liveweight gain at stocking rates above 25 sheep ha⁻¹, but had no effect on wool production at any stocking rate.
The results demonstrate that a stable and productive grass-clover association was maintained under conditions of set-stocking at around 23 yearling sheep ha⁻¹and that at this stocking rate, which appears to be about the biological optimum, there was no advantage in using N fertilizer.     

Reseeding in the Falkland Islands. Herbage production of a range of grass species and cultivars

The productivity of 20 cultivars from a range of 13 grass species was compared by harvesting four times during each of three successive growing seasons. In the experiment, 120 kg N ha⁻¹, 42 kg P₂O₅ ha⁻¹ and 42 kg K₂O ha⁻¹ were applied annually.
Mean annual yield was 3229 kg DM ha⁻¹ and 2147 kg DOM ha⁻¹. Cultivars were arranged in seven groups for comparison. Three of the groups, Lolium, Phleum and 'others' (the latter consisted of Festuca arundinacea cv. Dovey and Poa pratensis cv. Bensun A.34) appeared significantly lower yielding than the Dactylis, Festuca (fine-leaved), Agrostis and Holcus groups.
In the Lolium group, cv. Gremie outyielded cv. Melle every year. No cultivar of Dactylis was significantly better than any other in its group. Phleum CVS Motim and Eskimo were similar and superior to cv. S.50. Festuca ovina cv. Novina and Agrostis canina cv. Kingstown were marginally the best in their respective groups. The two cultivars of Holcus performed virtually identically. Festuca arundinacea cv. Dovey was a good producer of early spring grass. Poa pratensis cv. Bensun A.34 was generally low yielding and subject to fungal rust attack in autumn. The pattern of seasonal DM production was 16% in early spring (cut in October/November), 39% in mid-season (cut in December), 40% in late summer (cut in February/March) and 5% in autumn (cut in April/May). Quality of the herbage was superior to the main indigenous grass Cortaderia pilosa.
It was concluded that although the experiment was conducted under a cutting regime it provided useful guidance for seeds mixture formulation and stock carrying capacity calculations.     

Yield response of improved upland pasture to the control of leatherjackets under increasing rates of nitrogen

In each year from 1984-5 to 1987-8 the effect of leatherjackets on yield of improved upland pasture was assessed at Redesdale Experimental Husbandry Farm in Northumberland. Chlor-pyrifos at 0.72 kg ha⁻¹ was applied in November or December to half the plots; these and the untreated plots received one of four fertilizer rates (0, 40, 80, 120 kg N ha⁻¹). All rates increased yield by a mean of about 24 kg DM kg N⁻¹.
There was a significant correlation (Y (t DM ha⁻¹) = 0-21 + 0-00168 ×, r = 0-99 ( P = 0.001)) between yield response to chlorpyrifos and leatherjacket numbers m⁻² in November/ December. Treatment thresholds for leatherjacket numbers are given.     

The combined use of fertilizer nitrogen and white clover on grazed ryegrass swards

A 1-year grazing experiment with dairy cows is described in which the milk yield and herbage intake from a sward of S23 perennial ryegrass alone receiving fertilizer N at an annual rate of 360 kg ha⁻¹ were compared with those from a ryegrass-white clover var. Blanca sward given a total N application of 180 kg ha⁻¹. Both treatments gave virtually the same total milk yield of about 12500 kg ha⁻¹ over a 20-week grazing season.     

Forage availability from a temperate pasture managed with intensive rotational grazing

Successful integration of rotational grazing into livestock production systems requires estimates of pasture growth rates for feed budgeting of daily animal intake. By matching livestock nutrient demand with forage availability, over-feeding of supplements can be minimized, which reduces feed costs and the need lo manage surplus nutrients, A three-year grazing study was carried out on a Kentucky bluegrass ( Poa pratensis L.)-dominant pasture to estimate the daily quantity of herbage available to cattle in an intensive, rotational grazing system. Herbage production, species composition, and forage quality were determined in each of the six grazing cycles in a year, from April until September. The average length of a grazing cycle was 28·6 d, with 2·7 d for duration of grazing on a paddock. Pre-grazing and post-grazing sward heights, measured with a plate meter, were 14 and 7 cm, and the corresponding herbage masses were 1955 and 775 kg DM ha⁻¹ respectively. Under adequate soil moisture during 1989, herbage available for daily intake was 53 kg ha⁻¹ from April until mid- August, declining to approximately 32 kg ha⁻¹ d⁻¹ by the end of September. Distribution of this herbage was fairly uniform until the end of August. However, a dry summer in 1991 reduced herbage availability to 15 kg ha⁻¹ d⁻¹. Bluegrass and white clover ( Trifolium repens L.) formed 70% of the herbage yield during the period April–June. Later in the season, dead matter and other species increased, reducing the contribution of bluegrass and clover to approximately 60% of total dry matter. While these pastures have the potential to provide significant amounts of forage for 5–6 months in a year, additional on-farm forage reserves are needed during periods of water stress.     

Quantifying the effect of nitrogen fertilizer applications in spring on white clover content in perennial ryegrass-white clover swards

Data are presented for the fourth (1979) and fifth (1980) harvest years of a trial in which four levels of N fertilizer (0, 30, 60 and 90 kg N ha⁻¹) were applied each spring to swards with grass (perennial ryegrass cv. Barlenna) and each one of four cultivars of while clover (Blanca, Sabeda, Olwen and S100) or grass alone. Results from the first three years have been published.
Dry matter (DM) harvested dropped from the fourth to fifth years over all swards by 1 −5 to 2 0 t ha⁻¹ but response to N was maintained (17.4 and 24.4 kg DM (kgN)^−l in response to 90 kg N ha⁻¹ in 1979 and 1980 respectively) despite reductions in summer yields relative to unfertilized swards. Blanca swards produced significantly less DM harvested than all other cultivars in 1979 and all cultivars in 1980 except for Olwen. Clover DM harvested continued to fall from 1977, the mean for the unfertilized treatment in 1980 being 55% of that in 1979. Up to 1980 Sabeda swards produced more clover DM harvested than Blanca swards. Linear regressions between annual clover content at zero N and at each spring N level for each sward type over 5 harvest years were very highly significant. It is concluded that all cultivars used responded similarly to spring N. Further work to develop a method for predicting the effect of spring N on clover content of given swards is required.     

Response to late season nitrogen of upland swards in Wales

During 1984 and 1985, trials at sixteen sites throughout Wales measured the yield response in upland swards from applying 40 and 80 kg N ha⁻¹ at three dates, 20 August, 4 September and 18 September. All sites were between 230 m and 345 m above sea level. Grass yields were measured by cutting plots during October and November.
Nitrogen increased the herbage dry-matter (DM) yield at all sites in both years. The yield response per kg N applied varied between sites. Mean yield response for the two years declined from 16·2 kg DM kg N⁻¹ with 40 kg N ha⁻¹ applied on 20 August to 8·9 kg DM kg N⁻¹ with 80 kg N ha⁻¹ applied on 18 September. In general there was a decline in response to N with increased rate and delay in time of application. The results indicate that responses above an optimum of 9 kg DM kg N⁻¹ can be obtained well into September. A general rule that 1 kg N can be economically applied per 4 summed day degrees air temperature >6 °C remaining in the autumn up to 30 November is suggested.
Delay in application and increasing N rate increased crude protein in the herbage but had only small effects on modified acid detergent (MAD) fibre and sugar. Generally, grass quality was good. Sward density assessed during the spring after nitrogen had been applied appeared to have been unaffected by the treatments.