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利用间接血凝试验对来自河南省部分地区未接种过传染性胸膜肺炎疫苗的部分规模化猪场的807份血清样品进行了传染性胸膜肺炎的抗体检测,以抗体效价≥1∶8为阳性作为判定标准,结果发现被检样品的总阳性率为30.11%(243/807),经产母猪、40日龄断奶仔猪、90~120日龄猪和160~180日龄猪的阳性率分别为56.94%(123/216)、9.04%(17/188)、27.65%(60/217)、23.12%(43/186),上述各阶段阳性猪抗体效价的几何平均数分别为1∶65.15、1∶11.53、1∶26.92、1∶19.73。 相似文献
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北疆地区3个规模化猪场猪O型口蹄疫抗体监测结果及免疫程序分析 总被引:1,自引:0,他引:1
为了查明北疆地区3个规模化猪场O型口蹄疫病毒的抗体阳性率及免疫合格率,并提出针对该病的最佳免疫程序,分别采集北疆地区3个规模化猪场的公猪、后备母猪、哺乳母猪、妊娠母猪、7日龄、14日龄、21日龄、30日龄、40日龄和55日龄仔猪等不同猪群的血液,分离血清,共计1 500份。采用间接ELISA方法检测其抗体。结果显示,3个规模化猪场不同猪群的口蹄疫抗体阳性率及免疫合格率之间差异显著(P≤0.05),口蹄疫总体抗体阳性率为86.4%。3个猪场口蹄疫抗体阳性率分别为90.3%、90.6%、78.4%,不同猪群的抗体阳性率分别为公猪100%、后备母猪100%、哺乳母猪100%、妊娠母猪100%、7日龄仔猪91.9%、14日龄仔猪92%、21日龄仔猪87.6%、30日龄仔猪83.4%、40日龄仔猪73.9%、55日龄仔猪58.2%。综合本研究结果及该猪场前期的免疫程序,建议仔猪的口蹄疫免疫程序为40日龄首次免疫,54日龄二次免疫。 相似文献
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《黑龙江畜牧兽医》2017,(20)
为了给猪传染性胸膜肺炎制订合理的免疫程序提供依据,试验检测了仔猪接种两种猪传染性胸膜肺炎多价疫苗(1-2-7型)后的抗体消长规律。结果表明:接种两种不同猪传染性胸膜肺炎疫苗后,仔猪的抗体平均阻断率和阳性率变化趋势基本相同,在首免后第21天,仔猪传染性胸膜肺炎抗体平均阻断率和阳性率达到峰值,随后逐渐下降;二免后仔猪传染性胸膜肺炎的抗体平均阻断率和阳性率逐渐升高,在二免后第30天(即首免后第60天)达到第2次高峰,随后抗体平均阻断率逐渐下降,但仍维持在较高水平,抗体阳性率的变化幅度不大;1型胸膜肺炎放线杆菌抗体平均阻断率在首免后第44天略低于抗体保护临界值,抗体阳性率在首免后第37天稍高于合格率;7型胸膜肺炎放线杆菌抗体平均阻断率在首免后第30天时已经接近抗体保护的临界值。说明在猪的整个生长阶段,猪传染性胸膜肺炎多价疫苗需要免疫2次,且二次免疫的时间应在首免后第30~37天为宜。 相似文献
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对赤峰地区5个猪场送检的未免疫猪传染性胸膜肺炎疫苗的不同年龄阶段猪的338份血清样品,用间接血凝试验进行胸膜肺炎抗体检测。结果表明,所有猪场抗体均为阳性,平均阳性率63.15%;哺乳仔猪、育仔猪、生长育肥猪和成年种猪抗体阳性率分别达到3.23%、14.08%、91.14%、95.79%,总阳性率为52.07%。 相似文献
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[目的]掌握山西省猪传染性胸膜肺炎感染情况,为防控该病提供参考依据。[方法]用ApxIV-ELISA抗体检测试剂盒,对山西省11个市46个猪场851份猪血清进行猪胸膜肺炎放线杆菌ApxIV抗体血清学检测分析。[结果] 山西省猪胸膜肺炎放线杆菌抗体平均阳性率为57.11%;11个市猪场均有不同程度感染,感染率较高的是大同市、朔州市、忻州市,分别达到84.44%、83.75%和83.33%,临汾市感染率相对较低,为16.67%;母猪群体感染率最高,育肥猪次之,仔猪感染率最低;规模猪场感染率明显高于散养户感染。[结论] 猪传染性胸膜肺炎感染在山西省较为普遍,特别是母猪群阳性率达到74.60%,处在一个相对较高水平。 相似文献
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为了掌握昌吉地区5个规模化猪场猪瘟免疫抗体水平的高低及消长规律,同时为养殖户提供一个科学合理的免疫程序,本试验选择昌吉地区5个规模化猪场,收集2012~2013年不同年龄阶段的猪血清共1 350份,采用间接ELISA方法检测其抗体水平并进行分析。经过对昌吉地区5个规模化猪场共1 350份血清采用间接ELISA方法进行了检测,结果总抗体阳性率为80.07%(1 081/1 350),平均抗体滴度为8log2,标准差为0.856。其中A、B、C、D、E 5个规模化猪场猪瘟抗体阳性率分别为92.22%、82.59%、74.44%、88.51%、62.59%,通过对不同阶段猪群抗体阳性率结果统计可知公猪、后备母猪、哺乳母猪、妊娠母猪、7日龄仔猪、14日龄仔猪、21日龄仔猪、30日龄仔猪、40日龄仔猪、55日龄仔猪等不同阶段猪群的血清抗体阳性率分别为92%、69%、86%、93%、72%、82%、88.67%、80.67%、72.66%、82.67%。结合检测结果、临床生产数据及猪场已采用CSF的免疫程序,建议试验区仔猪猪瘟免疫程序为23~25日龄进行首免疫,60日龄时进行第二次免疫,生产公、母猪每年春秋两秋各普免一次,经产母猪每次断奶前跟胎免疫一次。 相似文献
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猪传染性胸膜肺炎是一种发病率较高的猪呼吸系统疾病,具有典型的胸膜肺炎和肺炎的病变特征与临床表现.猪传染性胸膜肺炎所导致的猪死亡率明显高于其他疾病,日龄在30~90之间的猪死亡率和发病率均高于20%,本次研究中,笔者对四个发生猪传染性胸膜肺炎的规模场进行了研究分析,对比试验猪传染性胸膜肺炎的灭活疫苗应用效果,将其分为90日龄以上育肥猪、30~90日龄仔猪、断奶前仔猪三组,并设置对照组,研究证实,注射灭活疫苗后发生猪传染性胸膜肺炎的仔猪在出栏前死亡率降低16.1%,发病率降低16.2%. 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献