Navy bean responses to zinc fertilizers

Abstract

Experiments were conducted to study the effects of various Zn carriers and rates on the growth and Zn uptake of Sanilac navy beans. Plants were grown under greenhouse and growth chamber conditions for 2, 4, 6 or 8 weeks on a Wisner silty clay loam soil low in available Zn. Plant growth responded to the various Zn fertilizers except at 4 weeks where no response was obtained. Only ZnZnEDTA and ZnNa₂EDTA increased growth at 2, 6 and 8 weeks while ZnSO₄, ZnHEIDA, ZnNTA and Zn(NO₃)₂ did not give consistent responses at all of these periods. The Zn carriers did not influence uptake until 6 weeks after planting.

Zinc rates began to influence plant growth at about the bloom stage, 6 weeks after planting with 2.0 kg/ha Zn as ZnNa₂EDTA and 3.3, 6.6 and 13.2 kg/ha Zn as ZnSO₄ giving higher responses than 0.7 or 1.3 kg/ha Zn as ZnNa₂EDTA. The most consistent increase in Zn uptake at 2, 6 and 8 weeks was obtained with 6.6 and 13.2 kg/ha Zn applied as ZnSO₄. No differences in uptake were obtained at 4 weeks between all the rates of Zn that were used. The data herein indicate that the stage of growth at which plant response to Zn fertilizers is evaluated may be very critical.     

Effects of ZnSO4 and Zn‐EDTA applied by broadcasting or by banding on soil Zn fractions and Zn uptake by wheat (Triticum aestivum L.) under greenhouse conditions

Zinc biofortification of staple food crops is essential for alleviating worldwide human malnutrition. Agronomic interventions to promote this should include fertilizer selection and management. A chelated Zn source, Zn‐EDTA, and an inorganic Zn source, ZnSO₄ × 7 H₂O, were applied either by banding or by broadcasting in soil, and Zn fractions in soil and Zn uptake by wheat were determined in a pot experiment. Compared to ZnSO₄ × 7 H₂O, Zn‐EDTA produced higher Zn concentration in grain regardless of application method and even at a lower application rate. Residual Zn fraction was the largest Zn fraction with both ZnSO₄ and Zn‐EDTA amendment. ZnSO₄ banded in soil caused Zn fractions to be restricted to the Zn‐amended soil band and resulted in lower grain Zn concentrations than did broadcast ZnSO₄. Planting wheat slowed Zn fixation by promoting the maintenance of a high concentration of Zn fraction loosely bound to organic matter (LOM‐Zn) in soil. Zn‐EDTA was a better Zn source for Zn biofortification of wheat than was ZnSO₄.     

锌源和施锌方法对石灰性土壤锌组分及锌肥利用率的影响

【目的】选用合适的锌肥以及合理的施肥方式不仅可以提高小麦籽粒锌营养品质,还可以提高石灰性土壤的锌肥利用率。因此,研究不同锌源和施肥方式对石灰性土壤中锌组分含量以及锌肥利用率的影响具有重要意义。【方法】采用盆栽试验,设置两种锌源(水溶态锌肥Zn SO4·7H2O和螯合态锌肥Zn-EDTA)全层混匀均施和表面条施两种方式,调查了土壤中交换态Zn(Ex-Zn)、松结有机态Zn(LOM-Zn)、碳酸盐结合态Zn(Carb-Zn)、氧化锰结合态Zn(Ox Mn-Zn)、紧结有机态Zn(TOM-Zn)5种形态锌的含量,分析了小麦对锌肥的利用率。【结果】全层混匀均施与表面条施,两种锌肥均增加了小麦籽粒和秸秆Zn含量,全层均施Zn SO4·7H2O处理的籽粒Zn含量比对照提高43%,均施和表面条施Zn-EDTA的籽粒Zn含量分别比对照提高57%和75%;Zn-EDTA均施和条施的锌肥利用率分别为6.5%和5.3%,Zn SO4·7H2O均施和条施的锌肥利用率分别为3.6%和1.3%。小麦收获后,条施Zn SO4·7H2O和Zn-EDTA的施锌区有效锌含量分别为9.25和1.97 mg/kg,分别为均施处理的2倍和1.8倍;与对照相比,Zn SO4·7H2O和Zn-EDTA条施及均施的4个处理均增加了土壤中各形态锌的含量,并且4个处理与对照土壤中各形态Zn含量的规律一致,即:松结有机态碳酸盐结合态紧结有机态氧化锰结合态交换态。均施Zn SO4·7H2O和Zn-EDTA 2个处理的交换态Zn含量分别为0.12和0.13 mg/kg,条施分别为0.38和0.54 mg/kg;均施处理松结有机态Zn含量分别为5.26和1.56 mg/kg;不同处理碳酸盐结合态Zn含量变化趋势与松结有机态Zn含量基本一致;条施Zn SO4·7H2O施肥区氧化锰结合态Zn含量为对照的4倍,不同处理的土壤中紧结有机态Zn含量变化规律与氧化锰结合态Zn含量变化规律相似。相关分析表明,土壤交换态Zn、松结有机态Zn和碳酸盐结合态Zn含量均与有效锌含量呈显著正相关关系,与不施锌肥相比,Zn-EDTA施入土壤后,小麦收获后松结有机态Zn和碳酸盐结合态Zn含量明显增加,而紧结有机态Zn则相对减少。【结论】潜在缺锌石灰性土壤上施用螯合态锌肥Zn-EDTA能显著增加土壤中潜在有效的锌组分以及锌肥利用率,而且施用螯合态锌肥后,较高的有效锌含量可以维持至小麦收获后,有效提高了锌肥利用率。与均施处理相比,条施这种集中施用的施肥方法可以增加近根系土壤中有效性较高的锌形态含量。     

Response of corn to plowed‐down and disked‐in Zn as zinc sulfate

Abstract

A field investigation was conducted to compare the efficacy of plowed‐down and disked‐in Zn as ZnSO₄.H₂O in correcting Zn deficiency of corn (Zea mays L.). The soil, Buchanan fine sandy loam, was nearneutral in pH and contained 0.7 ppm of EDTA‐extractable Zn and 1.4 ppm of dilute HCl‐H₂SO₄ extractable P. Application of 6.72 kg Zn/ha as ZnSO₄.H₂O corrected Zn deficiency of corn plants on the soil. Corn grain yields and Zn concentrations in tissue samples indicated that the plowed‐down and disked‐in Zn were about equally effective in correcting Zn deficiency where the level of Zn application was 6.72 kg/ha.     

Characterization of phytoavailable zinc in compost-peat substrates — Comparison of methods

Compost of separately collected green yard and organic household wastes may contain high amounts of Zn. Hence, substrates basing on compost can result in Zn phytotoxicity. The aim of the investigations was to identify a suitable reagent to characterize phytoavailable Zn in compost-peat substrates. Petunia hybrids were cultivated in compost-peat substrates with increasing Zn amounts (basic load, 400, 800, 1600 mg kg^?1 d.m. aqua regia soluble). Extractable Zn was determined by extraction of fresh and dried substrates with H₂O, 1 M NH₄NO₃, 0.1 M CaCl₂, 1 M NH₄OAc, and CaCl₂-DTPA (0.01 M + 0.002 M). The Zn content of plants increased significantly with increasing Zn application and decreasing pH. The result of CaCl₂ and NH₄OAc extraction reflected the influence of pH on Zn phytoavailability quite well and the correlation with the plant Zn content was very good. Extraction with H₂O and NH₄NO₃ did not give acceptable results. Extraction with CaCl₂-DTPA was inconsistent for all trials and thus not suitable. The percentage of CaCl₂-extractable Zn in relation to the aqua regia soluble content at the same pH varied over the trials. Thus, the phytoavailable content of the substrates is influenced by additional factors, besides Zn supply and pH. The result of CaCl₂ extraction of dried substrates resulted in the best reflection of Zn phytoavailability and was used for all further investigations, including determination of critical values of phytotoxic Zn. Plant yield was not influenced by the Zn treatments. However, Zn induced chlorosis of petunias occurred at a plant Zn content > 160 mg kg^?1 d.m. and a phytoavailable Zn content in the substrate > 6 mg l^?1 in CaCl₂ extract and > 2.6 mg l^?1 in NH₄OAc extract, respectively.     

The effectiveness of seed priming and foliar application of zinc- amino acid chelates in comparison with zinc sulfate on yield and grain nutritional quality of common bean

Abstract

The efficacy of seed priming and foliar application of zinc-amino acid chelates including zinc-histidine [Zn(His)₂] and zinc-methionine [Zn(Met)₂] in comparison with zinc sulfate (ZnSO₄) on yield and grain nutritional quality of two common bean cultivars (Phaseolus vulgaris L., cvs Talash and Sadri) was investigated in a severely Zn-deficient calcareous soil (DTPA-Zn: 0.38?mg kg^?1 soil) in a pot experiment. Bean response to Zn application varied depending on the Zn fertilizer, application method and cultivar. In ‘Talash’, seed priming with [Zn(His)₂] and [Zn(Met)₂] led to 24.1 and 11.6% increase in the grain yield of bean in comparison with ZnSO₄ treatment, respectively. In both cultivars, foliar application of [Zn(His)₂] led to significant increase in the grain yield in comparison with ZnSO₄. The highest grain Zn concentration was obtained by seed priming with [Zn(Met)₂] in ‘Sadri’ and [Zn(His)₂] in ‘Talash’, respectively. For Zn-amino acid chelates, seed priming was more effective than foliar application in increasing grain yield and Zn concentration. Foliar application of [Zn(His)₂] and [Zn(Met)₂] in ‘Sadri’ and [Zn(Met)₂] in ‘Talash’ resulted in higher protein content in bean grain as compared with ZnSO₄. In both cultivars, foliar application of [Zn(Met)₂] was the more effective than seed priming to increase grain protein content. The highest water-soluble carbohydrates concentration of grain was obtained by seed priming with [Zn(Met)₂] and [Zn(His)₂] in ‘Sadri’ and ‘Talash’ cultivars, respectively. Therefore, seed priming with [Zn(His)₂] and ZnSO₄ in ‘Sadri’ and [Zn(Met)₂] in ‘Talash’ can effectively be used for improving yield of common bean in Zn-deficient calcareous soils.     

Effects of Green Manures and Zinc Fertilizer Sources on DTPA-Extractable Zinc in Soil and Zinc Content in Basmati Rice Plants at Different Growth Stages

Rice is very sensitive to low zinc(Zn) supply in submerged paddy soils and Zn deficiency is one of the major limiting factors in determining rice production in India. A field experiment was conducted during the summer-rainy seasons of 2009 and 2010 at the research farm of the Indian Agricultural Research Institute, New Delhi, to determine the effects of summer green manure crops and Zn fertilizers on diethylenetriaminepentaacetic acid(DTPA)-extractable(available) Zn concentration in soil and total Zn content in Basmati rice cultivar Pusa Basmati 1 at periodic intervals. Summer green manure crops included Sesbania aculeata(Dhaincha),Crotalaria juncea(Sunhemp), and Vigna unguiculata(Cowpea) and the Zn fertilizers used were ethylenediaminetetraacetic acid(EDTA)-chelated Zn, ZnSO_4·7H_2O, ZnSO_4·H_2O, ZnO, and ZnSO_4·7H_2O + ZnO. Beneficial effects of summer green manure crops and Zn fertilizers on DTPA-extractable Zn concentration in soil and total Zn content in dry matter of Basmati rice at periodic intervals were observed, with significant increases in all the determined parameters, in comparison with those in the control(no Zn application or summer fallow). The rate of increase varied among summer green manure crops and Zn fertilizers during both years. Among the summer green manures, incorporation of S. aculeata led to a significant increase in mean Zn content in Basmati rice grain and straw when compared with C. juncea, V. unguiculata, and summer fallow treatments. Among the Zn fertilizers, significant increases in Zn content in Basmati rice dry matter and DTPA-extractable Zn concentration in soil during various growth stages of the plant were recorded with EDTA-chelated Zn application, followed by the application of ZnSO_4·7H_2O, ZnSO_4·H_2O, ZnSO_4·7H_2O + ZnO, ZnO,and no Zn. The highest mean Zn content in Basmati rice grain and straw was recorded with EDTA-chelated Zn application in 2009 and 2010, respectively. The application of ZnSO_4·7H_2O was the second best treatment after EDTA-chelated Zn; however, it was statistically inferior to EDTA-chelated Zn. The lowest values were recorded with the control(no Zn application) during both years of study. The amount of Zn concentration in soil was found to be significantly positively correlated with the Zn content in Basmati rice dry matter during both years. Significantly higher levels of residual fertility in soil after the harvest of Basmati rice were observed with application of EDTA-chelated Zn and incorporation of S. aculeata when compared with those of other Zn sources and summer green manures.     

伊朗西部地区碱性土壤中共离子对Zn吸附的影响

Zinc(Zn) is essential to plant growth and relatively mobile in soils.This study was conducted to assess the effect of common ions(Ca 2+,K +,Na +,NH + 4,Cl,NO 3,and H 2 PO 4) on sorption of Zn in surface samples of ten calcareous soils from western Iran using 10 mmol L 1 KCl,KNO 3,KH 2 PO 4,Ca(NO 3) 2,NaNO 3,and NH 4 NO 3 solutions as background electrolytes.The results indicated that both NH + 4,K +,and Ca 2+ equally decreased Zn sorption as compared to Na +.Zinc sorption was decreased by H 2 PO 4 as compared to NO 3 and Cl.The Langmuir and Freundlich equations fitted closely to the sorption data of all ions.The Langmuir maximum,bonding energy constant,and Freundlich distribution coefficient for Zn sorption differed among the various ionic background electrolytes.Langmuir sorption parameters showed that the presence of H 2 PO 4 decreased the maximum Zn adsorbed,but increased the bonding energy.Although K + and NH + 4 equally influenced maximum Zn adsorbed,they differed in their effect on the distribution coefficient of Zn in soils.Values of saturation index calculated using Visual MINTEQ indicated that at the low Zn concentration,Zn solubility was controlled by sorption reactions and at the high Zn concentration,it was mainly controlled by sorption and mineral precipitation reactions,such as precipitation of Zn 3(PO 4) 2.4H 2 O,Zn 5(OH) 6(CO 3) 2,and ZnCO 3.For most ionic background electrolytes,soil pH,CaCO 3,and cation exchange capacity(CEC) were significantly correlated with sorption parameters.     

Estimation of critical limit for zinc in rice soils

Abstract

Twenty surface soil samples wore collectod from Nalnltal Tarai (foot‐ hills of Himalya) where ‘Khaira’ disease (Zn deficiency disease of rice) was prevalent. Rice (Oryza sativa L. variety IR ‐ 8) was grown in pots for 8 weeks after transplanting. Experiments were carried out to find the suitability of five soil Zn extractants viz. dilute acid (HC1 ‐ H₂SO₄) mixture, DTPA‐ (NH₄) ₂CO₃, pH 7.3, dithizono, NH₄ ‐ Ac, pH 4.6 and MgCl₂. Critical limits of available Zn in soils were established for rice crop by old and new Cate and Nelson procedures.

Zn extracted from the soil with NH₄ ‐ Ac, pH 4.6, dithlzono, MgCl₂, and DTPA‐(NH₄) ₂CO₃, pH 7.3 was significantly correlated with the uptake of Zn by the rice plants. The correlation of Zn uptake with dilute acid mixture extractable Zn was not significant. The extractant which extracted more Zn gave higher values of critical limit and vice versa. It is concluded that all extracting solutions except dilute acid mixture were found to be suitable for predicting available Zn in rice soils of Taral.     

Estimation of critical limit for zinc in rice soils

Abstract

Twenty surface soil samples were collected from Nainital Tarai (foothills of Himalya) where “Khaira”; disease (Zn deficiency of rice) is prevalent. Rice (Oryza sativa L. variety IR‐8) was grown in pots for 8 weeks after transplanting. Experiments were conducted to determine the suitability of five soil Zn extractants: dilute acid (HCl + H₂SO₄) mixture; DTPA‐(NH₄) ₂CO₃, pH 7.3; dithizone; NH₄OAc, pH 4.6; and 2N MgCl₂ to predict Zn deficiency. Critical values for soil available Zn were established for rice by the old and new Cate and Nelson procedures¹.

Zinc extracted from the soils with dithizone; NH₄OAc, pH 4.6; 0.2N MgCl₂. and DTPA‐(NH₄) ₂CO₃ pH 7.3 was significantly correlated with the uptake of Zn by the rice plants. The correlation between Zn extracted with the dilute acid (HCl + H₂SO₄) mixture and plant Zn was not statistically significant. The ex‐tractants which extracted greater quantities of Zn gave higher critical values and vice versa. It is concluded that all extracting solutions except the dilute acid (HCl + H₂SO₄) mixture were found to he suitable for predicting available Zn in rice soils of Tarai.     

Use of routine soil tests in predicting corn leaf zinc

Abstract

Zinc of index corn leaves samples from 91 Minnesota sites on numerous soil types was correlated with soil Zn extracted by four routine procedures. The EDTA?(NH₄)₂CO₃ ‐ extractable soil Zn was more closely correlated with leaf Zn than soil zinc extracted by 0.1N HCl, EDTA‐NH₄OAc, or by NH₄OAc ‐ dithizone. Soil pH, CaCO₃ equivalent, extractable P, and organic matter of both acid and calcareous soils were negatively correlated with leaf Zn. When EDTA ? (NH₄)₂CO₃ ‐ extractable Zn was included with routine soil tests, a prediction equation for corn leaf Zn was formulated and compared with analytical values. However, the use of 1.4 ppm EDTA ? (NH₃)₂CO₃, ‐ extractable soil Zn alone as a critical value was equally effective in predicting leaf Zn.     

镁、锌、钼配施对银杏苗叶生物量和药用品质的影响

在温室条件下,采用正交试验设计,研究了叶面喷施镁、锌、钼对银杏苗叶生物量、主要药效成分含量及其单株产量的影响。结果表明:不同的镁锌钼组合对银杏苗叶生物量、总黄酮含量及其单株产量、萜内酯含量及其单株产量均有显著影响。较适宜银杏叶生物量、总黄酮含量、萜内酯含量、单株总黄酮产量、单株萜内酯产量提高的组合分别是Mg4Zn3Mo2、Mg1Zn4Mo2、Mg2Zn4Mo3、Mg4Zn4Mo2、Mg4Zn4Mo2。镁、锌、钼对银杏单株叶生物量、总黄酮含量及其单株产量影响的主次顺序:镁钼锌,对萜内酯含量影响的主次顺序:锌钼镁,对单株萜内酯产量的主次顺序:镁锌钼。叶面喷施镁锌钼肥可相应提高叶中镁、锌、钼含量,而叶面配施锌钼肥对叶中全镁的含量的影响不大,叶面配施镁肥在高水平下促进叶中全锌量积累,叶面配施钼肥在低水平（Mo2）下提高了叶中全锌含量,叶面配施镁锌肥对叶中全钼的含量影响不大。喷施镁肥对总黄酮含量产生了负面影响,但促进了萜内酯含量及其单株产量的提高;锌在高水平下对总黄酮和萜内酯含量有促进作用,低浓度下作用不大或有负面影响;钼在中低水平下对总黄酮和萜内酯含量有促进作用,高浓度下作用不大或有负面影响。     

Effect of summer green manuring crops and zinc fertilizer sources on productivity,Zn-uptake and economics of basmati rice

Field experiments were conducted for two consecutive years on basmati rice (Oryza sativa L.) during summer and rainy seasons (April–November) of 2009 and 2010 in a sandy clay-loam soil (typic Ustochrept) at the research farm of Indian Agricultural Research Institute, New Delhi. The aim of this study was to determine the influence of zinc fertilizer sources [ethylenediamenetetraacetic acid (EDTA)-chelated zinc (Zn; 12% Zn), zinc sulfate heptahydrate (ZnSO₄.7H₂O; 21% Zn), zinc sulfate monohydrate (ZnSO₄.H₂O; 33% Zn), zinc oxide (ZnO; 82% Zn), and ZnSO₄.7H₂O + ZnO (50% + 50%)] and summer green manuring crops [Sesbania aculeata, Crotalaria juncea and Vigna unguiculata] on productivity, Zn-uptake and economics of basmati rice. Among the summer green manuring crops, Sesbania aculeata accumulated highest amount of total dry matter, 5.46 and 5.77 t ha^?1 during 2009 and 2010, respectively. Incorporation of Sesbania aculeata also led to a significant increase in grain, straw and biological yields, Zn content and uptake and economics of succeeding basmati rice. With the incorporation of Sesbania aculeata, grain and straw yields of basmati rice was 4.89, 5.56 and 9.04, 10.21 t^?1 ha during 2009 and 2010, respectively. Among the Zn fertilizer sources, EDTA-chelated Zn (12% Zn) was found to be the best with respect to grain, straw, and biological yields, Zn content and uptake and economics. Application of EDTA-chelated Zn (12% Zn) recorded the highest basmati rice grain (5.15 and 5.76 t ha^?1) and straw yields (9.30 and 10.48 t ha^?1) compared to control (no Zn application), which produced 4.09 and 4.75 t ha^?1 of grain and 8.13 and 9.39 t ha^?1 of straw yields, respectively, during 2009 and 2010. Highest Zn concentration in grain and straw and its uptake was recorded with Sesbania aculeata incorporation compared with Crotalaria juncea, Vigna unguiculata and summer fallow. Highest total Zn uptake in basmati rice was recorded with EDTA-chelated Zn (12% Zn) application, followed by ZnSO₄.7H₂O (21% Zn), ZnSO₄.H₂O (33% Zn), ZnSO₄.7H₂O + ZnO (50% + 50%), ZnO (82% Zn), and control (no Zn application). Sesbania aculeata incorporation and EDTA-chelated Zn (12% Zn) treatments were found a better combination with respect to basmati rice productivity. The best economical returns were obtained with Sesbania aculeata incorporation and ZnSO₄.7H₂O (21% Zn) combination. Thus, adequate Zn fertilization along with green manure crop incorporation can lead to higher productivity of basmati rice.     

锌与磷肥混合方式对土壤中磷、锌有效性的影响

  【目的】  研究了锌与磷肥分别以物理混合和反应混合的方式结合后施用对土壤中锌、磷有效性的影响，为磷肥与锌肥的科学配施及高效利用提供科学依据。  【方法】  将锌肥 (ZnSO₄·7H₂O) 分别按0.5%和5%的质量比与磷酸氢二钾进行物理混合 (P+Zn) 和反应混合 (PZn) 后，制备含锌磷肥分别为P+Zn0.5、P+Zn5、PZn0.5和PZn5。利用X射线光电子能谱 (XPS) 和核磁共振波谱 (NMR) 分析锌与磷肥以不同方式结合对磷、锌化学结构和形态的影响；利用土壤培养试验，研究锌与磷肥以不同方式结合后施用对土壤有效锌和有效磷含量、土壤碱性磷酸酶活性及土壤pH的影响。试验设置8个处理:1) 不施任何肥料 (CK对照)；2) 施用普通磷肥 (P)；3)单施ZnSO₄·7H₂O 0.28 mg/kg (Zn0.5)；4) 单施ZnSO₄·7H₂O 2.81 mg/kg (Zn5)；5) 施用含锌磷肥P+Zn0.5 (P+Zn0.5)；6) 施用含锌磷肥P+Zn5 (P+Zn5)；7) 施用含锌磷肥PZn0.5 (PZn0.5)；8) 施用含锌磷肥PZn5 (PZn5)。其中，处理2)、5)、6)、7) 和8) 的磷用量相同，处理3)、5) 和7) 的锌用量相同，处理4)、6) 和8) 的锌用量相同。  【结果】  1) 与单施锌肥相比，锌与磷肥以物理混合 (P+Zn) 和反应混合 (PZn) 两种方式结合后施用均可提高土壤有效锌含量，4种含锌磷肥 (P+Zn0.5、P+Zn5、PZn0.5、PZn5) 分别使土壤有效锌含量提高了2.90%、12.17%、24.64%和10.86%，同样含锌量下反应制备的含锌磷肥 (PZn0.5、PZn5) 的效果比物理混合 (P+Zn0.5、P+Zn5) 的显著高出21.13%、7.37%。与PZn5处理相比，PZn0.5处理的土壤锌固定率可降低10.49个百分点。2) 土壤培养60天时，PZn0.5和PZn5处理的土壤有效磷含量较普通磷肥分别提高5.76%和5.70%，P+Zn0.5、PZn0.5和PZn5处理的土壤磷固定率分别降低了3.33、2.74和0.57个百分点。3) 在培养初始期，PZn0.5、PZn5处理可提高土壤碱性磷酸酶活性，以PZn0.5处理的效果好于PZn5处理，培养后期PZn0.5、PZn5处理可降低土壤pH，PZn0.5处理的降幅大于PZn5处理。  【结论】  施用物理混合或反应混合制备的含锌磷肥均可减少土壤对锌的固定，由于反应生成的含锌磷肥可降低土壤pH，其减少锌固定的效果优于物理混合，而且还可减少磷在土壤中的固定。以添加0.5%的硫酸锌反应制备的磷肥效果更好。     

Effect of Zn binding to phytate and humic substances on its uptake by wheat (Triticum durum L.) as affected by carbonates and Fe oxides

Although complexation with soil organic matter may improve zinc (Zn) bioavailability to plants, the effect of Zn sorbent surface on the use of complexed Zn by plants remains unknown. The objective of this research was to elucidate how Zn complexation with humic substances (HS) and phytate affects the uptake of Zn by wheat plants depending on the main sorbent surface in growth media, i.e., carbonates and Fe oxides. To this end, two pot experiments were performed, one using Fe oxide-coated siliceous as the siliceous growth medium sand and the other using a mixture of calcareous sand and siliceous sand as the calcareous growth medium. Each experiment involved three Zn sources, Zn-HS complex, Zn phytate, and ZnSO₄. All sources were applied with surface irrigation at two Zn rates (0.25 and 2 mg kg^-1 growth medium). The Zn-HS complex significantly increased Zn uptake by plants in both media, relative to the other two Zn sources, but no significant difference was observed between Zn phytate and ZnSO₄. In the calcareous medium, Zn-HS complex and Zn phytate resulted in significantly higher dry biomass yields of wheat than ZnSO₄. In the siliceous medium, spike and shoot dry biomass yields with Zn-HS complex at the low rate and Zn phytate at both rates were not significantly different from those with ZnSO₄ at the high rate. After harvest, approximately 50% of the Zn applied as Zn-HS complex remained extractable by diethylenetriaminepentaacetic acid (DTPA), while this proportion was less than 20% for the other Zn sources. Thus, Zn-HS complex and Zn phytate are sources of available Zn for plants, and they are more effective than ZnSO₄ in increasing plant growth, particularly when carbonates are the main Zn sorbent surface.     

锌肥品种与施用方法对水稻产量和锌含量的影响

【目的】 鉴于在缺锌土壤上施用锌肥不仅可提高水稻产量,而且也能显著提高水稻籽粒锌含量,本研究旨在揭示不同品种锌肥和施用方法在提高水稻产量和籽粒锌含量方面的差异。 【方法】 以水稻品种‘镇稻 11 号’为供试材料,于 2011 年 5 月至 2012 年 11 月在大田条件下设置 4 组试验:试验 1,富锌稻种的生产制备与籽粒锌含量的染色鉴定试验,设水稻常规种植(CK)和生育期连续喷施 5 次硫酸锌(ZnSO₄·7H₂O,Zn) 2 个处理;试验 2,不同品种锌肥土壤施用试验,设不施锌肥对照(S1)、土施 ZnSO₄·7H₂O (S2)、土施氮锌复合肥(Urea-Zn,S3)、土施磷锌复合肥(Mosaic-Zn,S4)、土施钾锌复合肥(Kali-Zn,S5)、富锌种苗移栽(S6)和 ZnSO₄·7H₂O 蘸秧根移栽(S7) 7 个处理;试验 3,不同品种锌肥叶面喷施试验,设不施锌肥对照(F1)、孕穗期喷施 ZnSO₄·7H₂O (F2)、开花期喷施 ZnSO₄·7H₂O (F3)、孕穗期和开花期各喷施 ZnSO₄·7H₂O (F4)、开花期与农药一起喷施 ZnSO₄·7H₂O (F5)、开花期喷施 Kali-EPSO-Zn(F6)和开花期喷施 ADOB-IDHA-Zn(F7) 7 个处理;试验 4,富锌稻种育苗种植比较试验,设常规稻种(CK-grain)和富锌稻种(Zn-grain)育苗种植 2 个处理,研究其对水稻产量及构成、各器官(叶片、茎秆和籽粒)锌含量和累积分配的影响。 【结果】 1) 在水稻生育期进行叶面高浓度和高强度的喷锌方式可制备富锌稻种,且可采用双硫腙(DTZ)染色直观鉴定籽粒锌含量。2) 施用锌肥显著提高水稻产量和锌含量,且不同锌肥品种和施用方法间差异显著。3) 土壤施锌各处理平均增产效果显著高于叶面喷锌 5.2%;与土壤施锌处理相比,叶面喷锌各处理的叶片、茎秆和籽粒锌含量的平均值则分别显著提高 224.6%、78.5% 和 12.1%。4) 在土壤施锌条件下,不同锌肥品种中氮锌复合肥 (S3) 和钾锌复合肥 (S5) 的增产效果显著高于磷锌复合肥 (S4),而在叶面喷锌条件下,以 Kali-EPSO-Zn 处理 (F6) 提高水稻各器官锌含量的能力显著高于 ADOB-IDHA-Zn 处理 (F7) 和 ZnSO₄·7H₂O 处理 (F3)。5) 较常规稻种 (CK-grain),应用富锌稻种 (Zn-grain) 育苗种植的水稻产量和籽粒锌含量分别提高 4.6% 和 3.9%。 【结论】 结合锌肥土施增加水稻产量和喷施增加籽粒锌含量的田间应用效果,因地制宜地综合采用土施氮锌复合肥和喷施钾锌复合肥的配合施肥方法以及选用富锌稻种育苗种植,可协同实现最佳的锌肥利用效率、最大程度地稳产增产和提高稻米的锌营养品质。      

Extractability of previously‐applied zinc as influenced by properties of calcareous soils

Abstract

The recovery of applied zinc (Zn) by plants is relatively small. Coupled with lack of leaching, this leads to accumulation of Zn in topsoil which may result in unfavorable growth conditions for the subsequent plants. Different extractants may be used for assessing the Zn status of soils previously treated with Zn sources. The extractability of retained Zn is influenced by soil properties. This experiment was conducted to study the influence of selected properties of calcareous soils on extractability of Zn by three popular Zn soil tests. Twenty samples from surface horizons (0–20 cm) of highly calcareous soils of southern Iran (pH 7.9 to 8.5; calcium carbonate equivalent 16 to 58%) previously treated with three levels of Zn (0, 10, and 20 mg Zn kg^‐1 soil as ZnSO₄#lb7H₂O) in triplicate and under one crop of corn (Zea mays L.) were extracted with DTPA, EDTA‐(NH₄)₂CO₃ and Na₂‐EDTA. Extractability (EXT) in a particular extractant was defined as the slope of the regression line, relating extractable Zn of each soil to the rate of applied Zn, multiplied by 100. The EXT values of soils ranged from 24.9 to 73.0% for DTPA, 47.2 to 84.4% for EDTA‐(NH₄)₂CO₃, and 28.2 to 56.7% for Na₂‐EDTA. Stepwise regression equations showed that cation exchange capacity (CEC) and calcium carbonate equivalent (CCE) followed by clay content were the most influential soil properties in EXT of retained Zn of highly calcareous soils. The EXT values decreased with increase in CEC, and CCE but increased with increase in clay.     

拔节期土壤施锌对小麦籽粒中锌生物有效性影响评估

本研究通过2012—2013及2013—2014两个年度的田间试验,在小麦(扬麦-16)的拔节期土壤施加高用量的锌肥,通过植酸/锌摩尔比法,对小麦籽粒进行了锌生物有效性评价,每公顷施ZnSO4·7H2O的量达到300 kg能显著提高小麦籽粒锌含量,小麦籽粒锌含量达到60 mg/kg以上,小麦籽粒植酸/锌摩尔比显著降低,植酸/锌摩尔比最大可降低至15以下,表明小麦锌强化后籽粒中锌的生物有效性得到显著提高。     

Zinc concentration of navy bean seed as affected by rate and placement of three zinc sources

Increased zinc (Zn) concentration in seed may sometimes improve human health. The influence of rate and placement of three Zn sources (ZnEDTA, ZnSO₄, and Rayplex‐Zn) on Zn concentration in navy bean (Phaseolus vulgaris L.) seed grown on a Calciaquoll was studied in the greenhouse. Application of 4 and 8 mg Zn/kg mixed throughout the soil increased seed Zn concentration by approximately 60 and 68%, respectively, and the responses were similar with the three Zn sources. The mixed‐throughout‐the‐soil ZnEDTA, ZnSO₄, and Rayplex‐Zn treatments applied at 8 mg Zn/kg reduced seed phosphorus (P) concentration by 10,13, and 15%, respectively. The corresponding reductions with 4 mg Zn/kg were 10, 8, and 13%, respectively. Banding ZnEDTA, ZnSO₄, and Rayplex‐Zn at 4 mg Zn/kg in 17‐cm‐long, 3‐cm‐deep bands reduced seed Zn concentration by 8, 34, and 31 % compared to their mixed‐throughout‐the‐soil counterparts. A significant Zn source x placement interaction resulted from marked reduction in Zn uptake as a result of banding ZnSO₄ and Rayplex‐Zn. Banding of ZnSO₄ and Rayplex‐Zn in calcareous soils is less likely to increase the Zn concentration of navy bean seed than is banding of ZnEDTA.     

Comparison of soil zinc extractants for detection of applied zinc and prediction of leaf zinc concentration

Abstract

Many soil extractants have been developed for determination of zinc (Zn) availability to plants. The optimum soil Zn extractant should be useful not only for prediction of plant Zn concentration but also for detection of applied Zn levels. The objectives of this study were: i) to compare soil Zn extradants for detecting applied Zn and for predicting peanut leaf Zn over a range of soil pH levels, and ii) to correlate other soil‐extractable Zn levels with Mehlich‐1. Soil and peanut leaf samples were taken from a field study testing pH levels as the main plots and Zn application rates in the sub‐plots. Extractable Zn was determined on soil samples using Mehlich‐1, Mehlich‐3, DTPA, MgNO₃, and many dilute salt extradants of varied strength and pH. Correlation of extractable soil Zn to cumulative applied Zn levels revealed Mehlich‐1, Mehlich‐3, DTPA, and AlCl₃ extradants to be among the best indicators of applied Zn. Leaf Zn concentration was best correlated with soil Zn extracted by dilute salts, such as KCl, CaCl₂, NH₄Cl, CaSO₄, and MgCl₂. Including soil pH as an independent variable in the regression to predict leaf Zn considerably improved R‐square values. The DTPA‐extractable soil Zn levels were very well correlated with Mehlich‐1‐extractable Zn. Mehlich‐3 extracted about 20% more soil Zn than Mehlich‐1, but Mehlich‐3 soil Zn was not as well correlated to Mehlich‐1 soil Zn as DTPA soil Zn. Lower pH solutions extracted more of the applied Zn, but more neutral solutions extracted Zn amounts which were better correlated with Zn uptake. On the other hand, Mehlich‐1, which had a lower pH, had better correlations with both applied Zn and leaf Zn than did Mehlich‐3. Shortening the DTPA extraction time to 30 minutes resulted in better correlations than the standard two hour extraction time. Chloride (Cl) was the best anion tested in relation to soil applied Zn recovery in combination with potassium (K), calcium (Ca), and aluminum (Al), and Cl optimized leaf Zn correlations for ammonium (NH₄), K, Ca, and magnesium (Mg). The larger the valence of the cation, the better the correlation with applied Zn and the poorer the correlation with leaf Zn.