植物生长调节剂激动素等在非洲菊组培中的应用

取 1ｃｍ左右的花蕾 ,分为 2～ 4块 ,接种到 3种分化培养基 :①含 6-苄基嘌呤 ( 6-ＢＡ) 2ｍｇ/Ｌ和萘乙酸 (ＮＡＡ)0 5ｍｇ/Ｌ的改良ＭＳ培养基 ;②含 10ｍｇ/Ｌ激动素的改良ＭＳ(ＫＴ)培养基 ;③含 6-苄基嘌呤 ( 6-ＢＡ) 10ｍｇ/Ｌ和吲哚乙酸 (ＩＡＡ) 0 5ｍｇ/Ｌ的改良ＭＳ培养基上。试验表明 ,在第三种培养基上几乎所有品种芽的分化均很好。在 2 5℃和 16ｈ的光照下培养 1～ 2个月可形成芽。将分化出芽的材料接种在增殖培养基 (附加ＫＴ 10ｍｇ/Ｌ或 6-ＢＡ 10ｍｇ/Ｌ的ＭＳ培养基 )中继代培养而大量增殖 ,在加ＫＴ的培养基上的幼苗较…     

增加培养基中蔗糖和盐的浓度能改善组培百合小鳞茎的生长

栽培品种Ｅｎｃｈａｎｔｍｅｎｔ的鳞片在改良ＭＳ培养基上形成的小鳞茎转移到含蔗糖 ( 30、60、90或者说 2 0ｇ/Ｌ)、0 1ｍｇＮＡＡ/Ｌ、0 1ｍｇ/Ｌ激动素养 0 4ｍｇ/Ｌ硫胺 (ＶＢ1)、10 0ｍｇ/Ｌ肌醇和 8ｇ /Ｌ琼脂的单倍或双倍浓度的ＭＳ培养基上 (ｐＨ5 7) ,在 2 5℃黑暗中培养 90ｄ后 ,在 90ｇ/Ｌ蔗糖的双倍浓度ＭＳ培养基上生长的小鳞茎体积和根系重量最大。增加培养基中蔗糖和盐的浓度能改善组培百合小鳞茎的生长     

非洲菊外植体的愈伤组织诱导和继代植株再生

取展开的非洲菊组培苗叶片做外植体 ,将其放入含有ＮＡＡ、ＢＡ、ＩＢＡ等不同激动素组合的ＭＳ培养基中培养。结果 ,在ＮＡＡ、ＢＡ和ＩＢＡ用量均为 1 0ｍｇ/Ｌ的ＭＳ培养基中愈伤组织生长最快 ,但仅在用量为ＮＡＡ 1ｍｇ/Ｌ ＢＡ0 75ｍｇ/Ｌ ＩＢＡ 0 75ｍｇ/Ｌ的ＭＳ培养基中有不定芽产生非洲菊外植体的愈伤组织诱导和继代植株再生     

FORUM ON "WOMEN AND SOCIAL FORESTRY"HELD BY THE FORESTRY AND SOCIETY NETWORK

ＦＯＲＵＭＯＮ“ＷＯＭＥＮＡＮＤＳＯＣＩＡＬＦＯＲＥＳＴＲＹ”ＨＥＬＤＢＹＴＨＥＦＯＲＥＳＴＲＹＡＮＤＳＯＣＩＥＴＹＮＥＴＷＯＲＫ￥ＢｙＬｉＷｅｉｃｈａｎｇＡｆｏｒｕｍｏｎ＂ＷｏｍｅｎａｎｄＳｏｃｉａｌＦｏｒｅｓｔｒｙ＂ｗａｓｈｅｌｄａｔｔｈｅＩｎｓ...     

“宁杞1号”组培快速繁殖技术研究

通过对“宁杞１号”离体组织培养进行了试管母本的建立、嫩梢增殖、生根状况分析及其移栽等技术研究，进而探索了提高快繁速度，降低试管苗成本以及适合生产上应用的组织培养技术。结果表明：改良ＭＳ附加ＢＡ０．２ｍｇ／ｌ＋ＫＴ０．３ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／ｌ和ＭＳ附加ＢＡ０．７５ｍｇ／ｌ＋ＮＡＡ１ｍｇ／ｌ＋ＩＢＡ０．１ｍｇ／ｌ两种培养基交替使用，可提高快速繁殖速度；以白砂糖代替培养基中蔗糖，能大大降低组培苗     

毛白杨转基因细胞系离体根培养及植株再生

为寻求解决木本植物离体根培养的途径，进行了毛白杨转基因细胞系离体根培养及植株再生研究。在组培条件下，毛白杨转基因细胞系Ｓ０１１２０３愈伤组织离体诱导根，生根百分率比毛白杨（对照）提高很多，高达１００％，根生长量比对照提高４～７倍。筛选了较适宜的固－液培养基，其配方为ＭＳ＋ＮＡＡ０．１ｍｇ·Ｌ－１（或ＩＢＡ０．５ｍｇ·Ｌ－１）。并研究了从Ｓ０１１２０３愈伤组织上切取不定根诱导植株再生的配套技术。诱导芽分化的培养基为ＭＳ＋６－ＢＡ０．５～１０ｍｇ·Ｌ－１，加低浓度的ＴＤＺ（０．０５～０．１ｍｇ·Ｌ－１）和ＩＢＡ（１．０ｍｇ·Ｌ－１）起促进作用，黑暗培养为好。     

刺梨汁SOD及其同工酶活力测定和定位染色

用透析法去除Ｖｃ测定刺梨汁ＳＯＤ活力操作简便,结果真实可靠,测得刺梨汁ＳＯＤ活力为５８．８Ｕ／ｍｌ。ＣＨＣｌ３－ＣＨ３ＣＨ２ＯＨ处理测得ＣｕＺｎ－ＳＯＤ３８．４Ｕ／ｍｌ,Ｈ２Ｏ２处理测得Ｍｎ－ＳＯＤ活力为１４．４Ｕ／ｍｌ,ＫＣＮ处理测得Ｍｎ－、Ｆｅ－ＳＯＤ活力为２２．８Ｕ／ｍｌ。聚丙烯酰胶凝胶电泳定位染色法证明刺梨汁具有ＣｕＺｎ－、Ｍｎ－、Ｆｅ－ＳＯＤ３种同工酶。     

枣树愈伤组织培养时不定芽的分化

以枣树色白、结构紧密的愈伤组织为材料,对其不定芽的分化情况进行了研究．结果表明,用ＭＳ（ＮＯ－３／２）作基本培养基,并附加ＫＴ４．０００ｍｇ／Ｌ,ＺＴ１．７５０ｍｇ／Ｌ和ＮＡＡ０．０１５ｍｇ／Ｌ可以获得比较好的不定芽分化效果,鸡蛋枣和无核小枣不定芽再生频率分别达到３０％,２２％,其它品种再生频率低于１５％,不定芽在ＭＳ（ＮＯ－３／２）＋ＮＡＡ０．０１５ｍｇ／Ｌ＋ＺＴ１．７５０ｍｇ／Ｌ＋ＫＴ４．０００ｍｇ／Ｌ＋ＡｇＮＯ３２．０００ｍｇ／Ｌ的培养基上能获得最高的增殖系数．     

《河北植物志》森林茜草的补正

《河北植物志》森林茜草的补正张素菊，冯天杰，张淑莲（河北林学院基础部保定０７１０００）关键词河北，森林茜草，补正中图分类号Ｓ７１８．３ＳＵＰＰＬＥＭＥＮＴＡＮＤＣＯＲＲＥＣＴＩＯＮＴＯＲＵＢＩＡＳＹＬＶＡＴＩＣＡＮＡＫＡＩ．ＩＮＦＬＯＲＡＯＦＨＥＢＥ...     

苦丁茶的组织培养研究

诱导苦丁茶具节茎段芽体发生、生长与增殖的培养基,以改良ＡｎｄｅｒｓｏｎＭＳ培养基＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ＋ＺＴ２．０ｍｇ／Ｌ＋ＧＡ３０．５ｍｇ／Ｌ较为适宜;以改良ＡｎｄｅｒｓｏｎＭＳ培养基＋ＢＡ３．０ｍｇ／Ｌ＋ＮＡＡ０．２５ｍｇ／Ｌ＋ＺＴ１．０ｍｇ／Ｌ继代培养。培养时温度为２８℃,光照度为１５００ｌｘ,光照时间为１４ｈ／ｄ;生根处理用１／４ＭＳ＋ＩＢＡ１．０ｍｇ／Ｌ培养基。     

毛白杨、银白杨嫁接育苗造林技术探讨

采用泰青杨和合作杨作砧木,进行毛白杨、银白杨育苗和造林试验。结果表明,用泰青杨作砧木,嫁接繁育毛白杨、银白杨,是培育毛白杨等较难生根杨树树种苗木的有效途径,并可提高育苗质量和造林成活率。     

山杨与美洲黑杨远缘杂交障碍克服的研究

以山杨为母本、美洲黑杨为父本,对克服山杨与美洲黑杨派间杂交障碍技术进行研究。结果表明：用正己烷有机溶剂熏柱头后再用山杨花粉提取液处理柱头能有效地克服山杨与美洲黑杨之间的杂交障碍。同时,用形态学标记对杂交子代及其亲本进行鉴定分析,杂种子代主要偏向于父本型与母本型,从而证明杂种子代是其真正的杂种。     

辽河杨、辽宁杨、盖杨在湖北省的引种试验

辽河杨、辽宁杨、盖杨三个品种于1997年引入湖北省,在不同的立地条件下进行育苗、造林实验。经过近10年的研究表明:辽宁杨、盖杨表现出了良好的适应性,生长量显著优于本地当前主栽品种中潜3号和中嘉8号,且材质好、抗性强;作为速生树种,在湖北湖区平原以及类似地区具有良好的发展前景。而辽河杨表现出对南方平原地区气候的不适应,宜在湖北省西北较干旱的丘陵岗地栽培推广。     

Gas exchange characteristics of Populus trichocarpa, Populus deltoides and Populus trichocarpa x P. deltoides clones

Responses of net photosynthesis, dark respiration, photorespiration, transpiration, and stomatal conductance to irradiance, temperature, leaf-to-air vapor density difference (VDD), and plant water stress were examined in two Populus trichocarpa clones (one from a moist, coastal climate in western Washington and one from a dry, continental climate in eastern Washington), one P. deltoides clone, and two P. trichocarpa x P. deltoides clones. Light saturation of photosynthesis in greenhouse-grown trees occurred at about 800 micromol m(-2) s(-1) for P. deltoides, P. trichocarpa x P. deltoides, and the eastern Washington ecotype of P. trichocarpa, but at about 600 micromol m(-2) s(-1) for the western Washington ecotype of P. trichocarpa. Average net photosynthesis (at saturating irradiance and the optimum temperature of 25 degrees C) was 20.7, 18.8, 18.2 and 13.4 micromol CO(2) m(-2) s(-1) for P. deltoides, P. trichocarpa x P. deltoides, and the eastern and western Washington clones of P. trichocarpa, respectively. In all clones, net photosynthesis decreased about 14% as VDD increased from 3 to 18 g H(2)O m(-3). Stomatal conductance decreased sharply with decreasing xylem pressure potential (XPP) in all clones except the western Washington clone of P. trichocarpa. Stomata in this clone were insensitive to changes in XPP and did not control water loss. Complete stomatal closure (stomatal conductance < 0.05 cm s(-1)) occurred at about -2.0 MPa in the eastern Washington clone of P. trichocarpa and around -1.25 MPa in the P. deltoides and P. trichocarpa x P. deltoides clones. Transpiration rates were highest in the P. trichocarpa x P. deltoides clone and lowest in the western Washington clone of P. trichocarpa. The P. deltoides clone and eastern Washington clone of P. trichocarpa had the highest water use efficiency (WUE) and the western Washington clone of P. trichocarpa had the lowest WUE. The hybrids were intermediate. It was concluded that: (1) gas exchange characteristics of eastern and western Washington clones of P. trichocarpa reflected adaptation to their native environment; (2) crossing the western Washington clone of P. trichocarpa with the more drought resistant P. deltoides clone produced plants better adapted to the interior Pacific Northwest climate, although the stomatal response to soil water deficits in the hybrid was conservative compared with that of the eastern Washington clone of P. trichocarpa; and (3) introducing eastern Washington clones of black cottonwood into breeding programs is likely to yield lines with favorable growth characteristics combined with enhanced WUE and adaptation to soil water deficits.     

克服胡杨与小叶杨远缘杂交障碍的研究

研究采用各种处理柱头的方法对胡杨(Populus euphratica)与小叶杨(Populus simonii)之间的杂交障碍进行研究,结果表明,用母本花粉提取液处理柱头的方法最为有效,其成苗率达到了73.68%,在此基础上对F1用回交、测交和自交的方法进行了遗传学分析,F1是真正的杂种,表明远缘杂交取得了成功。     

Populus tomentosa Carr

Populus tomentosa Carr, Chinese white poplar, is a large deciduous tree up to 30m tall and belongs to Section Leuce in Genus Populus. Family Salicacceae. Trunk is grey with irregular lenticels. Crown is conical to ovoidglobose, globose, or fastigiated; branchlets at first gray tomentose, glabrescent. Buds are ovoid, ovoid-globose to subglobose, tomentulose. Leaf blade is triangular ovate to deltoidovate, abaxially tomenrose when leaves unfold, glabrescent, margin with irregular teeth. Flowers are monoecious and aggregated in catkins.     

四季杨、南抗杨扦插育苗技术

四季杨(Populus spp.美洲黑杨×智利黑杨),1978年由中国林科院引入我国[1]。近于常绿,但在霜冻严重的寒冷地区落叶;该品种萌芽早,生长期长,生长速度快,产量高,尚未发现天牛危害,对食叶害虫抗性强,是短周期工业原料林、绿化防护林的优良树种。南抗杨(Populus deltoids Marsh)是我国自己选育出来的优良品种。具有生长快、成林易、成材早、产量高、质量好,适应性、抗逆性强、抗病虫害能力优,栽培技术简单等特点,是营造速生丰产、农田防护林、退耕还林、平原绿化、生态林等绿化美化的优良树种。随着短周期工业原林、农田防护林等多项工程建设…     

滇杨与美洲黑杨派间杂交育种试验初报

本研究以青杨派中的滇杨为母本,黑杨派中的美洲黑杨为父本,采用人工切枝水培杂交技术进行了滇杨×美洲黑杨的杂交工作,获得了一定数量的杂种苗。今后将结合表型性状和分子标记技术对杂种苗和亲本进行遗传变异分析,探索和研究滇杨×美洲黑杨杂交育种的遗传变异规律,亲子鉴定和早期选育的遗传基础。     

不同水分条件下胡杨和新疆杨苗期叶绿素变化

在不同水分条件下,应用SPDA-502叶绿素测定仪测定了胡杨和新疆杨苗木旺盛生长期的叶绿素含量.结果表明:(1)在7月和8月,不同水分处理下胡杨和新疆的叶绿素含量变化存在差异,7月胡杨叶绿素含量随着水分降低呈现增长趋势,而新疆杨叶绿素含量表现出先升后降再升高的趋势.8月叶绿素的含量变化是随着水分的减少2个树种都呈现先升...     

小×胡杨硬枝扦插试验初报

通过小X胡杨硬枝扦插试验,初步认为使用生根激素可以较明显提高插穗成活率,ABT1#生根粉效果最好,成活率较对照提高25.4%。激素在扦插当年对插穗生根起着关键作用,根系形成进入生长阶段后,继续生根不受激素影响。