共查询到8条相似文献,搜索用时 0 毫秒
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Mohammed ABDEL-HAKIEM Ayuko YAMASHITA Ayman ATIBA Yasuhiko OKAMURA Masaaki KATAYAMA Haroun YOUSSEF Hiroshi ISOMURA Yuji UZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):97-100
The pathogenesis of intervertebral disc (IVD) degeneration is not fully understood. The
biomolecular signaling pathways involved in the IVD degeneration require further
investigation. The aim of this study was to investigate the expression of NG2 proteoglycan
in the degenerated IVD. IVD samples were obtained from 16 Dachshunds that were confirmed
to have IVD herniation and subsequently underwent hemilaminectomy. The samples were
subjected to histological and immunohistochemical (IHC) examinations. IHC revealed
positive results for the expression of NG2 proteoglycan in all examined samples. The
results showed the expression of NG2 proteoglycan by the degenerated IVDs. 相似文献
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Ji-Yei CHOI Jung-Taek KANG Sol-Ji PARK Su-Jin KIM Joon-Ho MOON Islam M. SAADELDIN Goo JANG Byeong-Chun LEE 《The Journal of reproduction and development》2013,59(5):450-456
One of the factors that impairs in vitro produced porcine embryos
is the oxidative stress that is mainly caused by the imbalance between reactive
oxygen species (ROS) generation and antioxidants activity, especially that of
glutathione (GSH). Here, we examined the effect of 7,8-dihydroxyflavone (7,8-DHF), a
kind of flavonoid antioxidant, on porcine oocyte maturation and its developmental
competence. Porcine oocytes were cultured in media supplemented with 0, 1, 5 and 10
μM 7,8-DHF during both in vitro maturation (IVM) and in
vitro culture (IVC) after parthenogenetic activation. Maturation of
oocytes was evaluated based on first polar body (PB) extrusion and intracellular GSH
level, and developmental competence was assessed through observing cleavage and
blastocyst formation. In each step, the levels of intracellular GSH and ROS were
assessed by fluorescence intensity, and the apoptosis-related gene expression was
examined using semiquantitative RT-PCR. The group treated with 1 μM 7,8-DHF during
IVM and IVC showed increased cytoplasmic maturation and reached the blastocysts stage
(36.1%) at a higher rate than the other groups (24.7, 16.0 and 10.3% for 0, 5 and 10
μM, P<0.05). In that group, the intracellular GSH level was significantly
increased while ROS generation was significantly decreased after IVM and IVC
(P<0.05). Moreover, it showed high expression of an anti-apoptotic gene
(BCL2L1) and low expression of a pro-apoptotic gene
(BAK1) (P<0.05). In conclusion, treatment with 1 μM 7,8-DHF
during IVM and IVC showed an anti-apoptotic effect by increasing intracellular GSH
synthesis and scavenging ROS and therefore improved the developmental competence of
porcine embryos. 相似文献
4.
In vivo, resumption of oocyte meiosis occurs in large ovarian follicles after the
preovulatory surge of luteinizing hormone (LH). The LH surge leads to the activation of a broad signaling
network in mural granulosa cells equipped with LH receptors. The signals generated in the mural granulosa
cells are further augmented by locally produced peptides or steroids and transferred to the cumulus cell
compartment and the oocyte itself. Over the last decade, essential progress has been made in the
identification of molecular events associated with the final maturation and ovulation of mammalian oocytes.
All new evidence argues for a multiple roles of mitogen-activated protein kinase 3/1 (MAPK3/1) in the
gonadotropin-induced ovulation processes. However, the knowledge of gonadotropin-induced signaling pathways
leading to MAPK3/1 activation in follicular cells seems limited. To date, only the LH-induced transactivation
of the epidermal growth factor receptor/MAPK3/1 pathway has been described in granulosa/cumulus cells even
though other mechanisms of MAPK3/1 activation have been detected in other types of cells. In this review, we
aimed to summarize recent advances in the elucidation of gonadotropin-induced mechanisms leading to the
activation of MAPK3/1 in preovulatory follicles and cultured cumulus-oocyte complexes and to point out a
specific role of this kinase in the processes accompanying final maturation of the mammalian oocyte. 相似文献
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Yuuki ISAJI Koki YOSHIDA Hiroshi IMAI Masayasu YAMADA 《The Journal of reproduction and development》2015,61(6):503-510
In mouse somatic cell nuclear transfer (SCNT), polyvinylpyrrolidone (PVP) is typically included in the
nuclear donor injection medium. However, the cytotoxicity of PVP, which is injected into the cytoplasm of
oocytes, has recently become a cause of concern. In the present study, we determined whether bovine serum
albumin deionized with an ion-exchange resin treatment (d-BSA) was applicable to the nuclear donor injection
medium in SCNT as an alternative to PVP. The results obtained showed that d-BSA introduced into the cytoplasm
of an enucleated oocyte together with a donor nucleus significantly enhanced the rate of in
vitro development of cloned embryos to the blastocyst stage compared with that of a conventional
nuclear injection with PVP in SCNT. We also defined the enhancing effects of d-BSA on the blastocyst formation
rate when d-BSA was injected into the cytoplasm of oocytes reconstructed using the fusion method with a
hemagglutinating virus of Japan envelope before oocyte activation. Furthermore, immunofluorescence experiments
revealed that the injected d-BSA increased the acetylation levels of histone H3 lysine 9 and histone H4 lysine
12 in cloned pronuclear (PN) and 2-cell embryos. The injection of d-BSA before oocyte activation also
increased the production of cloned mouse offspring. These results suggested that intracytoplasmic injection of
d-BSA into SCNT oocytes before oocyte activation was beneficial for enhancing the in vitro
and in vivo development of mouse cloned embryos through epigenetic modifications to nuclear
reprogramming. 相似文献
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Peru Gopal BISWAS Yuma OHARI Uday Kumar MOHANTA Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):666
We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future. 相似文献
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Hiroyuki OHNISHI Shinya MIZUNO Yoko MIZUNO-HORIKAWA Takashi KATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):313-319
Ischemic acute kidney injury (AKI) is the most key pathological event for accelerating
progression to chronic kidney disease through vascular endothelial injury or dysfunction.
Thus, it is critical to elucidate the molecular mechanism of endothelial protection and
regeneration. Emerging evidence indicates that bone marrow-derived cells (BMCs) contribute
to tissue reconstitution in several types of organs post-injury, but little is known
whether and how BMCs contribute to renal endothelial reconstitution, especially in an
early-stage of AKI. Using a mouse model of ischemic AKI, we provide evidence that
incorporation of BMCs in vascular components (such as endothelial and smooth muscle cells)
becomes evident within four days after renal ischemia and reperfusion, associated with an
increase in stromal cell-derived factor-1 (SDF1) in endothelium and that in
CXCR4/SDF1-receptor in BMCs. Notably, anti-CXCR4 antibody decreased the numbers of
infiltrated BMCs and BMC-derived endothelium-like cells, but not of BMC-derived smooth
muscle cell-like cells. These results suggest that reconstitution of renal endothelium
post-ischemia partially depends on a paracrine loop of SDF1-CXCR4 between resident
endothelium and BMCs. Such a chemokine ligand-receptor system may be attributable for
selecting a cellular lineage (s), required for renal vascular protection, repair and
homeostasis, even in an earlier phase of AKI. 相似文献
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Ryota TERASHIMA Titaree LAOHARATCHATATHANIN Shiro KURUSU Mitsumori KAWAMINAMI 《The Journal of reproduction and development》2021,67(3):217
Functional relationship between nuclear receptor subfamily 4 group A member 3 (Nr4a3) and annexin A5 (Anxa5), which are two gonadotropin-releasing hormone (GnRH)-inducible genes, has been established while evaluating pituitary gonadotropes in relation to follicle-stimulating hormone beta (Fshb) expression. However, the physiological variations that arise due to the differential expression of these genes in the pituitary gland during rat estrous cycle remain unknown. This study aimed to evaluate the Nr4a3 and Anxa5 mRNA expression during the estrous cycle in rats in comparison with the expression of the gonadotropin subunit genes, luteinizing hormone beta (Lhb) and Fshb. Nr4a3 mRNA expression showed a single peak at 1400 h of proestrus during the 4-d estrous cycle. Anxa5 mRNA level was elevated along with increased Fshb mRNA expression after the decline of Nr4a3 mRNA until 2300 h. Lhb mRNA expression levels were not significantly changed during the estrous cycle. Notably, addition of a GnRH antagonist at 1100 h completely eradicated luteinizing hormone secretion at 1400 h and 1700 h of proestrus, and significantly reduced the Nr4a3 mRNA expression level at both the time points. These results suggest that GnRH is, at least partly, responsible for the increase in pituitary Nr4a3, and that the interaction between NR4A3 and ANXA5 is required to regulate Fshb expression during the preovulatory gonadotropin surge. 相似文献