共查询到20条相似文献,搜索用时 0 毫秒
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Margherita Orlandi Maurizio Mazzei Marta Vascellari Erica Melchiotti Claudia Zanardello Ranieri Verin Francesco Albanese Fabiano Necci Luca Pazzini Giulia Lazzarini Francesca Abramo 《Journal of veterinary diagnostic investigation》2021,33(6):1069
Numerous canine papillomaviruses (CPVs) have been identified (CPV1–23). CPV1, 2, and 6 have been associated with inverted papillomas (IPs). We retrieved 19 IPs from 3 histopathology archives, and evaluated and scored koilocytes, inclusion bodies, giant keratohyalin granules, cytoplasmic pallor, ballooning degeneration, and parakeratosis. IHC targeting major capsid proteins of PV was performed, and CPV genotyping was achieved by PCR testing. Tissue localization of CPV DNA and RNA was studied by chromogenic and RNAscope in situ hybridization (DNA-CISH, RNA-ISH, respectively). IPs were localized to the limbs (50%), trunk (30%), and head (20%), mainly as single nodules (16 of 19). In 15 of 19 cases, immunopositivity was detected within the nuclei in corneal and subcorneal epidermal layers. PCR revealed CPV1 in 11 IPs and CPV2 DNA in 3 IPs. Overall, 14 of 17 cases were positive by both DNA-CISH and RNA-ISH, in accord with PCR results. A histologic score >5 was always obtained in cases in which the viral etiology was demonstrated by IHC, DNA-CISH, and RNA-ISH. IHC and molecular approaches were useful to ascertain the viral etiology of IPs. Although IHC is the first choice for diagnostic purposes, ISH testing allows identification of PV type and the infection phase. RNA-ISH seems a promising tool to deepen our understanding of the pathogenesis of different PV types in animal species. 相似文献
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Vanessa MILECH Pmela CAYE Bernardo NASCIMENTO ANTUNES Josaine Cristina da SILVA RAPPETI Soliane CARRA PERERA Martielo Ivan GEHRCKE Thaís Cozza dos SANTOS Camila CONTE Fabrício de VARGAS ARIGONY BRAGA Guilherme Albuquerque de OLIVEIRA CAVALCANTI Maurício VELOSO BRUN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):618
This paper reports on two cases of laparoscopic nephrotomy employed in the treatment of canine dioctophymosis, which is considered a unusual procedure and a new treatment proposal heretofore not performed in veterinary medicine. Two patients were treated, one with a history of hematuria and the other with incidental finding of the parasite in the abdominal cavity during elective ovariohysterectomy. Both dogs were subjected to abdominal ultrasound, which produced images indicating the presence of the parasite in the right side kidney, but with partial parenchymal preservation. The patients were therefore subjected to laparoscopic nephrotomy. The surgical procedure was effective in treating dioctophymosis and enabled minimum tissue invasion during surgery, in addition to preservation of the kidney. 相似文献
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Katsuhito SONE Hideo AKIYOSHI Akiyoshi HAYASHI Fumihito OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):143-146
A 7-year-old female spayed Labrador Retriever was admitted to our hospital, because ofcough with sputum. She was diagnosed as having canine eosinophilic pneumonia (CEP) basedon blood eosinophilia, bronchial pattern and infiltrative shadow observed on thoracicradiography, bronchiolar obstruction and air-space consolidation predominantly affectingthe right caudal lung lobe, as revealed by computed tomography (CT), predominanteosinophils in CT-guided fine needle aspiration and the clinical course. She exhibited agood response to steroid therapy, and the cough disappeared. The serum surfactant protein(SP)-A level increased with the aggravated symptom and decreased markedly with improvementcompared with the C-reactive protein level and the number of eosinophils. We propose thatserum SP-A level is a good biomarker in CEP. 相似文献
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Alessio PIERINI Giada ESPOSITO Eleonora GORI Elena BENVENUTI Pietro RUGGIERO George LUBAS Veronica MARCHETTI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):248
Few studies have examined platelet alterations in dogs with chronic enteropathy. Our aim was to investigate platelet count (PLT), mean platelet volume (MPV), and platelet-to-lymphocyte ratio (PLR) in dogs diagnosed with immunosuppressant-responsive enteropathy (IRE). In this retrospective study of 41 dogs, data regarding signalment, canine chronic enteropathy clinical activity index (CCECAI), endoscopic and histopathological scores, PLT, MPV, PLR, total serum protein concentrations, albumin, and iron were collected. Clinical response and relapse were assessed with the evaluation of CCECAI over time. One month after starting therapy, dogs with >25% CCECAI reduction were considered responders. During a three-month CCECAI evaluation as part of a twelve-month follow-up, a CCECAI >3 together with a ≥2 unit increase in responder dogs was considered a relapse. PLT and PLR displayed significant negative correlation with MPV. MPV was positively correlated with total protein and albumin levels and negatively correlated with CCECAI. Three dogs were classified as non-responders, and 14 relapsed within 12 months. No differences were observed in PLT, MPV, or PLR between responding/non-responding and relapsing/non-relapsing groups. PLT, MPV, and PLR correlated with total protein, albumin, and CCECAI, confirming PLT as a potential marker, and suggesting MPV as a new marker of clinical efficacy against canine IRE. 相似文献
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Yoshiki YAMAYA Toshihiro WATARI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):421-425
Canine chronic rhinitis (CR) and bronchitis (CB) are suspected to be allergic diseases.
The present study tested whether dogs diagnosed with CR or CB present an atopic
predisposition based on the ratio of CC chemokine receptor 4 (CCR4)-positive cells among
peripheral blood CD4-positive cells (CCR4/CD4) and the serum levels of allergen-specific
IgE antibodies. We found that most dogs with CR and CB have a possibility of atopic
predisposition, and macrolide therapy constitutes an alternative to corticosteroid therapy
in controlling the clinical signs. 相似文献
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S. Murphy A. M. Hayes L. Blackwood G. Maglennon H. Pattinson A. H. Sparkes 《Veterinary and comparative oncology》2005,3(4):222-229
A retrospective study was undertaken of dogs presented to the Animal Health Trust for treatment of oral malignant melanoma, without radiographic evidence of pulmonary metastases. Group 1 (n = 13) received radiotherapy of the primary and any lymph node metastases (4 weekly fractions of 9 Gy); and group 2 (n = 15) were treated the same but additionally received between two and six doses carboplatin at 300 mg m?2 every 3 weeks. Median survival times for the two groups were 307 and 286 days, respectively (P > 0.05). In addition, carboplatin therapy did not significantly reduce the proportion of dogs dying due to metastases (three from group 1 and four from group 2). We found no evidence of a beneficial effect of carboplatin therapy over radiotherapy alone. 相似文献
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为了阐明树鼩精子中是否存在丝氨酸/苏氨酸蛋白磷酸酶1γ2(PP1γ2)及其在附睾精子中的存在形式,进而探究PP1γ2对精子成熟和运动性的调控作用,本试验以树鼩为研究对象,采用Western blotting分析了不同条件下树鼩附睾头和附睾尾精子中PP1γ2的存在形式和磷酸化程度,探讨了双丁酰环腺苷酸(db-cAMP)、3-异丁基-1-甲基黄嘌呤(IBMX)或Ca2+对树鼩精子中PP1γ2磷酸化表达水平的影响,并进一步研究了磷酸酶抑制剂冈田酸(okadaic acid,OA)和花萼海绵诱癌素A (calyculin A,CA)对树鼩精子中PP1γ2磷酸化程度的影响及其对树鼩附睾头和附睾尾精子运动度的影响。结果显示,在树鼩附睾头和附睾尾精子中均存在PP1γ2,且在等量的附睾头和附睾尾精子蛋白中,PP1γ2在附睾尾精子的磷酸化程度远高于附睾头精子;db-cAMP、IBMX或Ca2+不改变PP1γ2的磷酸化水平;磷酸酶抑制剂OA和CA能明显提高附睾头和附睾尾中PP1γ2磷酸化的程度,且能显著提高精子(尤其是附睾头精子)的运动度(P<0.05),OA和CA的最佳作用浓度分别为1 μmol/L和10 nmol/L,最佳作用时间分别为15、20 min。本研究结果表明,蛋白磷酸酶PP1γ2对树鼩精子成熟及运动性具有重要的调控作用,其主要通过磷酸化和去磷酸化的变化发挥作用。 相似文献
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Jun TAMURA Tomohito ISHIZUKA Sho FUKUI Norihiko OYAMA Kodai KAWASE Kenjiro MIYOSHI Tadashi SANO Kirby PASLOSKE Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):289-296
The pharmacological effects of the anesthetic alfaxalone were evaluated after
intramuscular (IM) administration to 6 healthy beagle dogs. The dogs received three IM
doses each of alfaxalone at increasing dose rates of 5 mg/kg (IM5), 7.5 mg/kg (IM7.5) and
10 mg/kg (IM10) every other day. Anesthetic effect was subjectively
evaluated by using an ordinal scoring system to determine the degree of neuro-depression
and the quality of anesthetic induction and recovery from anesthesia. Cardiorespiratory
variables were measured using noninvasive methods. Alfaxalone administered IM produced
dose-dependent neuro-depression and lateral recumbency (i.e., 36 ± 28 min, 87 ± 26 min and
115 ± 29 min after the IM5, IM7.5 and IM10 treatments, respectively). The endotracheal
tube was tolerated in all dogs for 46 ± 20 and 58 ± 21 min after the IM7.5 and IM10
treatments, respectively. It was not possible to place endotracheal tubes in 5 of the
6 dogs after the IM5 treatment. Most cardiorespiratory variables remained
within clinically acceptable ranges, but hypoxemia was observed by pulse oximetry for 5 to
10 min in 2 dogs receiving the IM10 treatment. Dose-dependent decreases in rectal
temperature, respiratory rate and arterial blood pressure also occurred. The quality of
recovery was considered satisfactory in all dogs receiving each treatment; all the dog
exhibited transient muscular tremors and staggering gait. In conclusion, IM alfaxalone
produced a dose-dependent anesthetic effect with relatively mild cardiorespiratory
depression in dogs. However, hypoxemia may occur at higher IM doses of alfaxalone. 相似文献
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Kosei SAKAI Tomohiro YONEZAWA Hideyuki YAMAWAKI Toshifumi OYAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1319-1322
Somatostatin receptor 2 (SSTR2) is a negative regulator of cell proliferation in human
breast cancer. Since there is little information about SSTR2 in canine mammary gland tumor
(MGT), we clarified its distribution and expression level in normal mammary gland, benign
MGT and malignant MGT. SSTR2 expression determined by immunohistochemical staining was
observed in the cytoplasm of luminal epithelial cells. The intensity was negatively
correlated with malignancy: normal tissues and some of the benign tumors had the highest
levels, while the malignant tumors had little or no SSTR2 expression. As for the Western
blotting, SSTR2 protein level in benign tumors was significantly lower than the normal
mammary gland. On the other hand, SSTR2 protein levels in two of three malignant tumors
were higher than the other groups. These results suggest that SSTR2 expression alters
according to the malignancy of canine MGT. 相似文献
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Masaya IGASE Chung Chew HWANG Matt COFFEY Masaru OKUDA Shunsuke NOGUCHI Takuya MIZUNO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):541-548
Oncolytic virotherapy is a new strategy for cancer treatment for humans and
dogs. Reovirus has been proven to be a potent oncolytic virus in human medicine. Our
laboratory has previously reported that canine mast cell tumor and canine lymphoma were
susceptible to reovirus. In this study, canine solid tumor cell lines (mammary gland
tumor, osteosarcoma and malignant melanoma) were tested to determine their susceptibility
towards reovirus. We demonstrated that reovirus induces more than 50% cell death in three
canine mammary gland tumors and one canine malignant melanoma cell line. The
reovirus-induced cell death occurred via the activation of caspase 3. Ras activation has
been shown to be one of the important mechanisms of reovirus-susceptibility in human
cancers. However, Ras activation was not related to the reovirus-susceptibility in canine
solid tumor cell lines, which was similar to reports in canine mast cell tumor and canine
lymphoma. The results of this study highly suggest that canine mammary gland tumor and
canine malignant melanoma are also potential candidates for reovirus therapy in veterinary
oncology. 相似文献
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The inducible inflammatory enzyme cyclooxygenase‐2 (COX‐2) and its product prostaglandin E2 (PGE2) are prominent tumour promoters, and expression of COX‐2 is elevated in a number of tumours of both humans and canines. Targeting COX‐2 in cancer is an attractive option because of readily available non‐steroidal anti‐inflammatory drugs (NSAIDs), and there is a clear epidemiological link between NSAID use and cancer risk. In this study, we aim to establish the anti‐tumourigenic effects of the selective, long‐acting COX‐2 inhibitor mavacoxib. We show here that mavacoxib is cytotoxic to a panel of human and canine osteosarcoma, mammary and bladder carcinoma cancer cell lines; that it can induce apoptosis and inhibit the migration of these cells. Interestingly, we establish that mavacoxib can exert these effects independently of elevated COX‐2 expression. This study highlights the potential novel use of mavacoxib as a cancer therapeutic, suggesting that mavacoxib may be an effective anti‐cancer agent independent of tumour COX‐2 expression. 相似文献
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Comparative analysis of MAPK and PI3K/AKT pathway activation and inhibition in human and canine melanoma 下载免费PDF全文
The lack of advanced animal models of human cancers is considered a barrier to developing effective therapeutics. Canine and human melanomas are histologically disparate but show similar disease progression and response to therapies. The purpose of these studies was to compare human and canine melanoma tumours and cell lines regarding MAPK and PI3K/AKT signalling dysregulation, and response to select molecularly targeted agents. Pathway activation was investigated via microarray and mutational analysis. Growth inhibition and cell cycle effects were assessed for pathway inhibitors AZD6244 (MAPK) and rapamycin (PI3K/AKT) in human and canine melanoma cells. Human and canine melanoma share similar differential gene expression patterns within the MAPK and PI3K/AKT pathways. Constitutive pathway activation and similar sensitivity to AZD6244 and rapamycin was observed in human and canine cells. These results show that human and canine melanoma share activation and sensitivity to inhibition of cancer‐related signalling pathways despite differences in activating mutations. 相似文献
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灌溉及多效唑对蓝茎冰草生长及种子产量的影响 总被引:1,自引:1,他引:1
在甘肃省酒泉地区研究灌溉和多效唑对蓝茎冰草(Agropyron smithii)种子产量及其构成要素的影响。结果表明,蓝茎冰草从返青到收获,返青期、拔节期、抽穗期、灌浆期、盛花期灌溉5次水的生殖枝数/m2、小穗数/生殖枝和种子产量最高,但每个时期的灌溉量不宜过于充足,以防止植株倒伏;拔节期灌溉有利于生殖枝数/m2和种子数/小穗的增加,生殖枝数/m2和种子数/小穗与种子产量之间相关性极显著;返青期一次性喷施0.022 5%的多效唑0.3 kg/hm2对提高生殖枝数/m2、小穗数/生殖枝、小花数/小穗、种子数/小穗以及种子产量效果显著;灌施多效唑有效地提高了种子数/小穗,最终也提高了种子产量。多效唑喷施的效果较灌施好。 相似文献
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Elizabeth A. Wood Zhanping Lu Shuai Jia Anna L. F. V. Assumpo Matthew A. Van Hesteren Mike K. Huelsmeyer David M. Vail Xuan Pan 《Veterinary and comparative oncology》2020,18(3):269-280
MLN4924 (pevonedistat) is a potent and selective NEDD8‐activating enzyme (NAE) inhibitor. The NEDD8‐regulated neddylation system is responsible for the regulated degradation of intracellular proteins with important cellular functions in cancer cell growth, apoptosis, angiogenesis and metastasis. In human melanoma, inhibition of NAE results in induction of DNA re‐replication, S phase cell cycle arrest, DNA damage and apoptosis. The study aimed to assess the anti‐cancer effect of MLN4924 on canine malignant melanoma cell lines and patient samples and to elucidate the underlying mechanisms. Canine melanoma cell lines and primary patient samples were evaluated for cell viability after incubation with varying concentrations of MLN4924 or dimethyl sulfoxide. Apoptosis, cell proliferation and senescence assays were performed to address underlying mechanisms of MLN4924‐mediated anti‐tumour effects. Gene expression of seven previously identified deregulated genes in human melanoma was compared in sensitive vs resistant samples. MLN4924 treatment significantly reduced the viability of canine melanoma cell lines and primary samples in a dose‐ and time‐dependent manners. MLN4924 promoted cell apoptosis and inhibited cell growth through induction of DNA re‐replication and cell senescence. While the majority of canine melanoma samples demonstrated sensitivity at nanomolar ranges, some samples were resistant to the treatment. Modulation of P21 levels correlated with canine melanoma cell sensitivity. These results provided justification for further exploration of MLN4924 as a treatment of canine melanoma. 相似文献
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Mizuki TOMIHARI Akira NISHIHARA Terumasa SHIMADA Masashi YANAGAWA Masafumi MIYOSHI Kazurou MIYAHARA Akihiro OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1227-1233
Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity,
and research shows that total intravenous anesthesia (TIVA) with propofol has a greater
immuno-protective effect than inhalational anesthesia in human medicine. However, in
veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on
propofol and isoflurane, investigating clinical blood hematology and immunological
profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy
adult beagles were included in this study, randomly assigned to the propofol anesthesia
group (group P: n=6) or the isoflurane anesthesia group (group I: n=6). In both groups,
the number of lymphocytes in peripheral blood decreased after 2 hr of anesthesia (2 hr),
but group P showed significantly less decrease than group I. For T-lymphocyte subsets
examined by flowcytometry, the ratio of CD3+, CD4+ and CD8+ lymphocytes in the peripheral
blood mononuclear cell (PBMC) of group P at 2 hr also exhibited a high level compared to
group I. Moreover, for mRNA expression of cytokines measured by real-time PCR, the IL2
(pro-inflammatory cytokine) of group P showed no decrease like group I. The IL10
(anti-inflammatory cytokine) of group P also showed no increase like group I, while both
cytokines maintained nearly the same level until 2 hr. These results suggest that,
compared to propofol, isoflurane had more strongly immuno-suppression caused by
anesthesia, and propofol itself might have some immuno-protective effects. Thus, TIVA with
propofol might benefit immunological support in the perioperative period of dogs. 相似文献