Effects of collembola on nutrient cycling and microbial biomass on intact soil microcosm

(Jpn. J. Soil Sci.Plant Nutr., 77, 299–306, 2006)

The effects of Collembola (Folsomia candida Willem) on nutrient cycling, microbial biomass, and soil respiration were studied using intact soil microcosms. Intact soil microcosms (dia. 10·6 cm and depth 15 cm) were taken from pine forest soil, and were divided into four treatments · the unmanipulated control and three Collembolan manipulations in which microcosms were defaunated by deep-freezing, and then F. candida were introduced at three densities (0, 50, 100 per microcosm). The microcosms were incubated on forest floor with a roof. At 3- to 4-week intervals the microcosms were irrigated with deionized water for analyses of nutrients (Na+, K+, NH4+, Ca2+, Mg2+, Cl?, NO3?, SO42?) in the leachate. Soil respiration was measured using an infrared gas analyser. After 13 and 34 weeks of exposure, microcosms were destructively sampled. Collembola did not significantly affect microbial biomass C, N, and P nor soil respiration. Because the experiment was started in winter, nutrient leaching increased from spring to summer with increasing microbial activity. At the end of the experiment, leached nitrate from microcosms was significantly different between the 0 and 50 Collembolan treatments. Total established Collembolan biomass was under 4% of the soil microbial biomass in the microcosms, while manipulation of Collembola affected soil nitrogen dynamics at high microbial and collembolan activity.     

Effects of nutrients omission on maize growth and nutrient uptake in three dominant soil types of southwestern Nigeria

Abstract

A pot experiment was conducted to determine most limiting nutrients for maize performance using nutrient omission treatments in three soil types of southwestern Nigeria. There were six treatments; full nutrient [120?kg nitrogen (N)/ha, 40?kg phosphorus (P)/ha, 80?kg potassium (K)/ha, 10?kg molybdenum (Mo)/ha, and 5?kg zinc (Zn)/ha]; full nutrient minus N, P, K, Mo, and Zn including control was replicated thrice. Treatments were arranged as split plot in a complete randomized design. Data were collected on growth parameters, shoot, root dry weights, and NPK uptakes. Data were subjected to analysis of variance and means separated using LSD_0.05. Majeroku and Egbeda soils and full nutrient supported better maize growth and NPK uptakes. Shoot weight was higher in Egbeda while root weight was higher in Itagunmodi soil. Phosphorus was the most limiting in Egbeda and Itagunmodi soils, and nitrogen in Majeroku soil. In conclusion, maize growth, nutrient uptake and most limiting nutrient varied with soil types.     

菌根化育苗对玉米生长和养分吸收的影响

【目的】丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)侵染作物根系形成菌根共生体系对于作物吸收磷具有重要作用,但该结果大多来源于室内受控试验,有限的田间试验因环境条件、试验材料与接种技术等差异致使AMF菌剂应用效果不一。本研究通过玉米菌根化育苗和田间移栽,分析了接种AMF对玉米生长、养分吸收、籽粒产量及养分含量的影响,以期推进菌根技术的实际生产应用。【方法】以自交品系玉米B73为供试作物,于2018年5月至10月在北京市延庆区进行了田间试验。田间小区设置基施磷(+P)和不施磷(–P)处理。供试AMF为Rhizophagus irregularis Schenck&Smith BGC AH01。玉米种子催芽后,分别播入加入AMF菌剂(+M)和菌剂过滤液(–M)的育苗钵内,培养两周后移栽至田间。玉米在田间条件下生长至拔节期时,使用便携式光合仪测定叶片光合速率与气孔导度,取样测定地上部与根部干重和养分元素含量,同时测定菌根侵染率;在玉米完熟期取样,测定籽粒百粒重、籽粒产量及养分含量。【结果】无论田间施磷与否,接菌植株根系的菌根侵染强度和丛枝丰度均显著高于不接菌植株。不施磷情况下,+M处理显著提高了玉米根系干重,玉米生长的菌根依赖性(163.7%)显著高于施磷情形(124.1%)。–P–M处理玉米叶片的光合速率和气孔导度显著低于其他3个处理。–P+M处理玉米叶片的光合参数、玉米地上部和根部磷含量与+P+M均无显著差异。与–P–M处理相比,–P+M显著提高了玉米籽粒产量和百粒重,同时也提高了籽粒中锌、锰、镁等矿质养分的含量,且与+P+M处理相比均无显著差异。【结论】玉米幼苗接种AMF后再移栽到田间,可以显著提高拔节期玉米根系的菌根侵染率,促进玉米地上部和根部对磷及锌、锰和镁的吸收,进而促进玉米的生长,提高籽粒产量和养分含量。本试验条件下,菌根化育苗可以达到与施磷同样的效果,在保障作物不减产的前提下减少磷肥施用量。     

放牧对荒漠草原植物生物量及土壤养分的影响

以宁夏荒漠草原为研究对象,探讨放牧对荒漠草原植物多样性、 生物量及土壤养分特征的影响。结果表明, 放牧对荒漠草原植物群落多样性、 均匀度和丰富度影响显著。植物群落多样性和均匀度随着放牧强度的增加均呈先增加后降低的趋势,在轻度放牧达到最大值。同围封禁牧相比,重度、 中度和轻度放牧草地的植物地上和地下部生物量显著降低,分别降低了43.8%、 42.0%、 15.4% 和 27.7%、16.2%、11.9%。土壤有机碳随着放牧强度的增加而降低,而土壤全氮含量随着放牧强度的增加呈先增加后降低的趋势。围封禁牧草地土壤有机碳比重度放牧增加了18.1%,而土壤全磷、 速效磷和全钾含量分别降低了 21.1%、 51.9% 和 11.0%。土壤有机碳含量对植物群落地上和地下部生物量的影响大于土壤全氮、 全磷、 全钾、 速效磷和速效钾。放牧干扰下荒漠草原土壤环境及其养分含量,能在一定程度上反映植物群落多样性和生物量的变化。     

生物碳对土壤磷素和棉花养分吸收的影响

通过两年温室盆栽试验,研究了不同磷肥用量下生物碳对土壤磷素含量、 棉花生长和养分吸收的影响。试验以棉花秸秆为原料制备生物碳,制成三种热解温度（450℃、 600℃和750℃）的生物碳,分别以BC450、 BC600和BC750表示,同时以空白土壤为对照（CK）; 磷肥（P2O5）用量设3个水平0、 0.25、 0.5 g/kg（分别以P0、 P1、 P2表示）。研究结果表明,施用生物碳可显著提高土壤磷素含量及其有效性,随着生物碳热解温度的升高,土壤水溶性磷、 速效磷及全磷含量均显著增加,且对三种磷素含量的影响表现为水溶性磷 全磷 速效磷。施用生物碳处理两年棉花的干物质重均显著高于对照,但不同热解生物碳处理对两年棉花干物质重的影响各异。施用生物碳可显著增加棉花养分吸收量,总体表现为750℃ 600℃ 450℃。因此,施用生物碳可显著提高土壤磷素含量,促进棉花生长和养分吸收; 热解温度是影响生物碳质量的重要因素,生物碳的热解温度越高（450~750℃）,其促进作用越好。     

Micro nutrient uptake of glasshouse cucumbers grown on rockwool

Abstract

The uptake of micro elements by cucumbers grown on rockwool was investigated in a series of experiments. The elements studied in the experiments consisted of iron, manganese, zinc, boron, copper and molybdenum. Five different levels of each of these elements were compared.

The deficiency and excess symptoms were described and the fruit yields were recorded. The trace element contents of the nutrient solution in the root environment were determined by regular sampling. From time to time, samples were also collected of the leaves and fruits of the cucumber crop.

No relationship appeared to exist between the iron contents in the root environment and those in the leaves and fruits. However, relationships were found for all other elements included in the investigation. The relationship between the contents in the root environment and in the crop is curvilinear and can be compute roughly by a function of the model y = ax^b, in which x represents the content of the element in the root environment and y represents the content in the crop.

The limits are given at which deficiency and excess symptoms may be expected.     

缓冲液种类对土壤酸性磷酸酶活性的影响

土壤酸性磷酸酶影响土壤有机磷矿化。选择不同的缓冲液种类和不同p H值测定土壤酸性磷酸酶活性,可影响数据的可比性。为明确缓冲液及p H值对不同土壤磷酸酶活性的影响程度,选取p H值4～8的土壤样本,测定了在醋酸盐缓冲液、柠檬酸盐缓冲液和MUB缓冲液3种不同培养条件下酸性磷酸酶活性变化。结果表明,采用MUB缓冲液条件可获得较稳定的土样区分序列,且与缓冲液的p H值的变化无关。在醋酸盐缓冲液和柠檬酸盐缓冲液条件下,不同p H值范围的不同土样之间土壤酸性磷酸酶活性变异系数变化剧烈,而采用MUB缓冲液获得的变异系数较其他缓冲液大,且趋势平稳。综合考虑,进行土壤酸性磷酸酶活性测定,可优先选用MUB缓冲溶液。     

Nutrient mobilization and nutrient contents of Zea mays in response to EDTA additions to heavy‐metal‐contaminated agricultural soil

Enhanced phytoextraction of heavy metals (HMs) using chelating agents and agricultural crops is widely tested as remediation technique for agricultural soils contaminated with less mobile HMs. Nutrients are complexed by chelating agents simultaneously to HMs. In this study, the effect of EDTA (ethylenediaminetetraacetic acid) application on nutrient mobility in the soil and nutrient contents of Zea mays was tested on the laboratory and on the field scale. EDTA effectively increased the mobility of total water‐soluble macronutrients (Ca, K, Mg, P) and micronutrients (Fe, Mn) in the soil solution. Thereby nutrient co‐mobilization did cause competition to target HMs during the phytoextraction process. Mobilization was caused by complexation of nutrient cations by negatively charged EDTA and by dissolution of oxides and hydroxides. Increased concentrations of negatively charged P indicate the dissolution of metal phosphates by EDTA. Higher total water‐soluble nutrient concentrations enhanced bioavailability and plant contents of all determined nutrients especially that of Fe. Mobilization of nutrients may result in leaching and loss of soil fertility.     

Short‐term effect of biochar and compost on soil fertility and water status of a Dystric Cambisol in NE Germany under field conditions

Crop growth in sandy soils is usually limited by plant‐available nutrients and water contents. This study was conducted to determine whether these limiting factors could be improved through applications of compost and biochar. For this purpose, a maize (Zea mays L.) field trial was established at 1 ha area of a Dystric Cambisol in Brandenburg, NE Germany. Five treatments (control, compost, and three biochar‐compost mixtures with constant compost amount (32.5 Mg ha^–1) and increasing biochar amount, ranging from 5–20 Mg ha^–1) were compared. Analyses comprised total organic C (TOC), total N (TN), plant‐available nutrients, and volumetric soil water content for 4 months under field conditions during the growing season 2009. In addition, soil water‐retention characteristics were analyzed on undisturbed soil columns in the laboratory. Total organic‐C content could be increased by a factor of 2.5 from 0.8 to 2% (p < 0.01) at the highest biochar‐compost level compared with control while TN content only slightly increased. Plant‐available Ca, K, P, and Na contents increased by a factor of 2.2, 2.5, 1.2, and 2.8, respectively. With compost addition, the soil pH value significantly increased by up to 0.6 (p < 0.05) and plant‐available soil water retention increased by a factor of 2. Our results clearly demonstrated a synergistic positive effect of compost and biochar mixtures on soil organic‐matter content, nutrients levels, and water‐storage capacity of a sandy soil under field conditions.     

盐渍土番茄苗期养分吸收特性的研究

本文研究了盐渍土下番茄苗期养分吸收特性及土壤含盐量对番茄苗期养分吸收的影响.结果表明随苗期的增长,番茄对磷钾需求量增加幅度加大,养分吸收量由氮最高转变为钾,其排列为K2O＞N＞P2O5＞CaO＞MgO.苗期后期养分吸收量为全苗期吸收总量的80%左右.随土壤含盐量的增加,抑制番茄苗期生长,对氮、磷、镁的吸收影响不大,对钾和钙的吸收有明显的抑制作用.     

地下部分隔对间作小麦养分吸收和白粉病发生的影响

通过根系分隔的盆栽试验,探讨了地下部分隔对小麦//蚕豆间作系统中小麦的养分吸收和白粉病发生的影响。结果表明:根系不分隔小麦的生物量和N、P、K吸收量均大于尼龙网分隔处理和根系完全分隔处理。根系分隔方式还影响了小麦白粉病的发生,小麦白粉病的发病率和发病指数均表现为根系不分隔(I)尼龙网分隔(M)完全分隔(P)。表明地下部的相互作用改善了小麦的生长,提高了小麦抗白粉病的能力。     

Effect of plant growth-promoting bacteria and soil compaction on barley seedling growth, nutrient uptake, soil properties and rhizosphere microflora

Inoculants are of great importance in sustainable and/or organic agriculture. In the present study, plant growth of barley (Hordeum vulgare) has been studied in sterile soil inoculated with four plant growth-promoting bacteria and mineral fertilizers at three different soil bulk densities and in three harvests of plants. Three bacterial species were isolated from the rhizosphere of barley and wheat. These bacteria fixed N₂, dissolved P and significantly increased growth of barley seedlings. Available phosphate in soil was significantly increased by seed inoculation of Bacillus M-13 and Bacillus RC01. Total culturable bacteria, fungi and P-solubilizing bacteria count increased with time. Data suggest that seed inoculation of barley with Bacillus RC01, Bacillus RC02, Bacillus RC03 and Bacillus M-13 increased root weight by 16.7, 12.5, 8.9 and 12.5% as compared to the control (without bacteria inoculation and mineral fertilizers) and shoot weight by 34.7, 34.7, 28.6 and 32.7%, respectively. Bacterial inoculation gave increases of 20.3–25.7% over the control as compared with 18.9 and 35.1% total biomass weight increases by P and NP application. The concentration of N and P in soil was decreased by increasing soil compaction. In contrast to macronutrients, the concentration of Fe, Cu and Mn was lower in plants grown in the loosest soil. Soil compaction induced a limitation in root and shoot growth that was reflected by a decrease in the microbial population and activity. Our results show that bacterial population was stimulated by the decrease in soil bulk density. The results suggest that the N₂-fixing and P-solubilizing bacterial strains tested have a potential on plant growth activity of barley.     

The effect of the function type for describing the soil buffer power on calculated ion transport to roots and nutrient uptake from the soil

The relationship between amount of a nutrient in soil and its concentration in solution, the buffer curve, is usually non‐linear. However, most models that calculate nutrient transport in the soil and nutrient uptake by the plant often assume a linear buffer curve because of simpler programming. In this paper a model is presented that uses the Freundlich function to describe the non‐linear buffer curve in the soil. It has been shown that calculated uptake and depletion curves were not influenced by the shape of the buffer curve. In a sensitivity analysis the buffer capacity, soil solution concentration, soil water content, soil impedance factor, maximum influx and the curvature of the Freundlich function were varied and the influence on calculated uptake was studied. The buffer capacity in general had a minor influence and the linearity or non‐linearity of the buffer capacity had under no circumstances an influence on calculated uptake. Thus, the use of a linear buffer curve in transport models does not lead to a significant error in the results.     

崩岗侵蚀对土壤速效养分质量分数及化学计量比的影响

为揭示崩岗侵蚀对土壤速效养分及化学计量比的影响,选取福建省长汀县黄泥坑崩岗群微度、中度和强度崩岗3处为研究对象,测定和分析了0 ～ 30 cm土层集水坡面、崩壁、崩积体以及沟道部位的土壤基本物理性质、速效养分及化学计量特征.结果表明:1)随着侵蚀强度的增加,硝态氮和速效磷质量分数表现为强度＞中度＞微度,中度侵蚀崩岗铵态氮质量分数最低,速效钾质量分数最高;从水平方向上,各速效养分质量分数表现为速效钾(AK)＞铵态氮＞硝态氮＞速效磷(AP);垂直方向上,各速效养分质量分数大致随着土层深度而降低,但同一侵蚀强度内各质量分数差异较小.2)土壤速效氮为铵态氮与硝态氮的总和(AN),AN/AP比表现为微度＞强度＞中度,微度与中度差异显著(P ＜0.05);AK/AN比为中度＞微度＞强度,中度与强度差异显著(P ＜0.05);AK/AP比为微度＞中度＞强度,微度与中度差异显著(P＜0.05).3)从集水坡面、崩壁、崩积体至沟道,3种类型崩岗的铵态氮和硝态氮平均值都呈降低的趋势,速效磷逐渐升高,速效钾在崩积体处最低,其他3个部位变化不大;土壤AN/AP、AK/AP比呈降低的趋势,AK/AN比在沟道处最高.4)速效养分化学计量比与砂粒、粉粒、pH和有机质皆密切相关,黏粒、容重和含水率对其影响相对较小.     

腐植酸改良铜污染土壤对小麦生长与吸收利用养分的影响

为了评价腐植酸对铜污染土壤的改良效果,以冬小麦为研究对象,设置施加铜和腐植酸双因素田间盆栽试验,研究成熟期小麦的干物质积累与分配以及对氮、磷、铜素的吸收与利用。结果表明:(1)铜污染土壤中小麦器官的生长受到抑制,总生物量显著降低28.7%～66.9%;小麦吸收氮、磷量分别显著降低18.4%～61.5%、9.6%～65.5%,氮、磷素收获指数显著下降;尽管铜污染使小麦对铜素的吸收量显著增加40.5%～115.2%,但茎向叶和穗的迁移系数下降,使得收获指数无变化。(2)施用腐植酸明显改善铜污染造成的小麦根、茎、穗生长受到的抑制,缓解小麦吸收氮、磷素减少的不利影响;相比铜污染处理,腐植酸低(20g/kg干土)和高(40g/kg干土)用量可使小麦生物量分别提高25.0%和115.6%,促进小麦对氮(22.6%～111.9%)、磷(28.3%～161.9%)的吸收并提高氮、磷素的收获指数。高腐植酸用量更有助于小麦抵抗铜污染危害胁迫。(3)增施腐植酸能使小麦铜素收获指数显著降低7.2%～9.0%,但会显著增加铜素由茎向叶(12.2%～30.7%)和由茎向穗(46.8%～71.9%)的迁移,导致铜在穗中的积累量增加。由此可见,施用腐植酸改良铜污染土壤的农用效果仍需要进一步研究。     

基于地统计的土壤养分采样布局优化

传统的土壤养分采样布置方法都是基于采样区土壤特征状态空间随机变异的假设。而地统计学研究表明,土壤特征状态在空间上有关联性,因此利用传统方法来制定采样方案并不是最优的,因为它没有考虑土壤特性的空间相关性,不能反映其局部的变化特征。该文在分析土壤肥力空间变异的基础上,研究利用经典统计学方法确定合理的采样点数目,并基于地统计学的半方差函数拟合与Kriging方法确定合理的采样点布局的方法,选择典型地区的土壤肥力进行空间变异分析和采样点布置的优化设计。研究结果表明：在合理的位置布置14个采样点就可以满足典型基地种植区绘制施肥处方图进行变量施肥决策的要求;利用经典统计学与地统计分析相结合的方法进行农田尺度的土壤肥力采样布点优化分析具有良好的可行性。     

施肥对菜园土壤养分淋溶流失浓度的影响

利用盆栽种植空心菜和菜心并收集测定土壤渗漏水氮磷钾养分浓度,研究了不同施肥水平对土壤氮磷钾淋溶流失浓度的影响。试验表明,在较大施肥量或化肥当季损失量范围内,土壤渗漏水硝态氮、总磷和钾浓度与氮、磷、钾施肥量或损失量之间呈非线性关系,但在中低施肥量时则表现为线性关系。化肥当季损失量与土壤渗漏水养分浓度呈显著水平的线性正相关,由此提出“双速率转折点”概念用以评价土壤养分流失潜力。当施肥量或化肥当季损失量超过双速率转折点X0后,土壤淋溶排水养分浓度将以非线性形式急剧增加。因此,从环保角度看,施肥量不应超过X0。盆栽试验表明,以化肥当季损失量为基础的灰泥土、灰黄泥土和黄泥土的氮肥X0分别为每盆N 0.35、0.32和0.34 g,磷肥X0分别为每盆P 0.06、0.06和0.09 g,灰泥土和灰黄泥土的钾肥X0则分别为K 0.17和0.18 g;与土壤渗漏水养分浓度为基础所确定的氮、磷、钾X0有显著水平的线性正相关。以环保为目标的氮磷钾用量X0大多数低于以产量为目标的经济施肥量。化肥当季损失量参数较易获得,可作为环保施肥的依据。     

连续施用酸化粪水对土壤养分淋溶及重金属累积的影响

为探索连续施用酸化粪水对土壤养分淋溶及重金属累积情况的影响,采用新鲜粪水和酸化粪水,开展土柱淋溶试验。试验分别设置1个对照组、新鲜粪水和3个不同pH值（6.5、6.0和5.5）的酸化粪水,每个处理分别设置6次粪水淋溶。结果表明：施用新鲜粪水和酸化粪水均能增加土壤养分,施用新鲜粪水、pH值6.5、pH值6.0和pH值5.5的粪水后土壤总养分（N、P、K）的增长幅度分别为1%～40%、15%～66%和5%～21%,重金属Cu和Zn的增长幅度为4%～48%和4%～11%,重金属Cd和Pb的增长幅度为2%～14%和1%～18%;连续施用酸化粪水会使土壤pH降低、土壤电导率值升高以及土壤重金属不断累积,这也是导致土壤环境遭到破坏的风险因素,实际应用过程中应特别注意;建议每两季作物施用一次pH值为6.5的粪水;每三季作物施用一次pH值为6.0的粪水;每四季作物施用一次pH值为5.5的粪水。该研究通过对比分析连续施用新鲜粪水和不同pH值的酸化粪水后土壤养分和重金属浓度的变化,探讨了酸化粪水的还田效果,为连续施用酸化粪水的研究提供技术支撑。     

土壤紧实胁迫对黄瓜生长、产量及养分吸收的影响

用容重分别为1.2、1.4和1.6.g/cm3的土壤进行盆栽试验,研究了土壤紧实度对黄瓜生长、产量及养分吸收的影响。结果表明,当土壤紧实度增大时,黄瓜秧苗的株高在定植后的15.d后受到显著抑制;第4叶的叶宽和叶长在定植后9～17.d内增加;茎粗则是在稍紧的土壤中(R.1.4)最大,过紧的土壤中(R.1.6)最小;根系伸长生长受阻,干物质质量及活力显著下降,根冠比降低;生物学产量、经济产量、经济系数的变化情况及植株对氮、磷、钾吸收量的变化与茎粗的变化趋势相同。在本试验条件下,容重为1.2.g/cm3的土壤利于株高及根系的生长,容重1.4g/cm3的土壤则利于茎粗、根系养分的吸收及产量的增加。