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对虾皮下及造血组织坏死杆状病毒单克隆抗体的研制 总被引:7,自引:1,他引:7
用经密度梯度离心纯化的对虾皮下及造血组织坏死杆状病毒(HHNBV—937)注射Balb/c小鼠,取其免疫后的脾细胞与sp2/0骨髓瘤细胞进行融合。经筛选与克隆,获得7株抗HHNBV的单克隆杂交瘤细胞株。其中F9株的染色体数为87,ELISA测定培养上清中单抗滴度为1∶8,腹水为1∶1000。患有杆状病毒的皮下及造血组织坏死症的对虾组织切片的单抗酶联免疫染色,表明F9株单抗只与HHNBV病灶发生特异性结合,与对虾组织结构不发生特异性反应。将其用于现场样品的检测研究表明,该抗体适于用来进行对虾暴发性流行病的病原检测。 相似文献
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不同家系的万氏对虾对对虾杆状病毒抗病力的差异 总被引:2,自引:1,他引:2
不同家系的万氏对虾对对虾杆状病毒抗病力的差异郑国兴(东海水产研究所,上海200090)关键词对虾杆状病毒,万氏对虾,抗毒力,家系DIFFERENCEINRESISTANCETOEXPERIMENTALINFECTIONSWITHBACULOVIRUS... 相似文献
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INGESTIONANDEXCRETIONOFSEVERALANTIBACTERIALDRUGSBYTHEBRINESHRIMP(ARTEMIAPARTHENOGENETICA)1.TOXICITYT
INGESTIONANDEXCRETIONOFSEVERALANTIBACTERIALDRUGSBYTHEBRINESHRIMP(ARTEMIAPARTHENOGENETICA)1.TOXICITYTOARTEMIA,INGESTIONANDEXCR... 相似文献
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对虾暴发性白斑病毒病综述 总被引:1,自引:0,他引:1
到目前为止,国内虾病工作者 达成了基本的共识,即造成对虾暴 发性流行病的病原体是一种无包 涵体病毒。但由于不同学者研究方 法及侧重点不同,对此病毒的叫法 不一、分别为:“白斑杆状病毒 (White spot baculovitus,WSBV)” “皮下造血组织坏死杆状病毒 (Hypodermal and Hematopoitetic Necrosis Baculovirus, HHNBV)” “对虾白斑综合病(white spot syndrome virus WSSV)”、“对虾 杆状DNA病毒(PRD… 相似文献
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鄱阳湖渔区的团头鲂胚胎发育观察 总被引:5,自引:1,他引:4
鄱阳湖渔区的团头鲂胚胎发育观察ORSERVATIONSONTHEEMBRYONICDEVELOPMENTOFMEGALOBRAMAAMBLYCEPHALAINTHEFISHINGAREASOFPOYANGLAKE虞鹏程张丰旺(南昌大学生物科学工程系,... 相似文献
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大口黑鲈仔、稚鱼生长和食性的观察 总被引:6,自引:0,他引:6
大口黑鲈仔、稚鱼生长和食性的观察陆伟民(上海水产大学,200090)关键词大口黑鲈,仔、稚鱼,生长,食性OBSERVATIONONGROWTHANDFEEDINGHABITSOFLARGEMOUTHBASS,MICRORTERUSSALMOIDES,... 相似文献
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罗氏沼虾幼体及成虾消化酶活性 总被引:19,自引:0,他引:19
罗氏沼虾幼体及成虾消化酶活性魏华,赵维信(上海水产大学,上海200090)关键词罗氏沼虾,幼体,消化酶THEACTIVITIESOFDIGESTIVEENZYMESOFLARVAEANDADULTINMACROBRACHIUMROSENBERGII¥... 相似文献
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温度与盐度和缢蛏幼体生存、生长及发育的关系 总被引:12,自引:0,他引:12
本文以长期生活在较高盐度(26—28‰)海域的亲蛏所繁衍的幼体为材料,探索温度和盐度单因子及双因子结合与幼体生存生长、发育的相互关系。浮游幼虫期适盐范围为8.4—32.4‰,最适盐度为12.4‰,稚贝适盐范围为8.4—28.4‰,最佳盐度为12.4‰。温度对幼体的影响,是受盐度高低所支配。在盐度为28.4‰生境中培育,浮游幼虫期适温范围为17—25℃,最适温度为21℃,稚贝适温范围为13—25℃,最适温度为17℃,幼体对25℃以上的较高温度的忍耐力较差。若在最适盐度(12.4‰)中培育,浮游幼虫期最适水温为25℃,稚贝最适水温为21℃。幼虫对较高盐度的忍耐力比对较低盐度的忍耐力来得强。 相似文献
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George W. Chamberlain Addison L. Lawrence 《Journal of the World Aquaculture Society》1981,12(1):207-224
A variety of natural foods have been reported to induce ovarian maturation of penaeid shrimp. In this study, conducted from 19 June to 11 September 1980, four single-food diets (clams, shrimp, squid, and worms) and one composite diet consisting of all four foods were fed to Penaeus vannamei in 3.7-m diameter indoor circular tanks. The shrimp, averaging 28 g, were stocked at a rate of 6.9 m-2 with a 1:1 male to female ratio. Unilateral eyestalk ablation was performed on half of the females in each tank. Tanks received one turnover of water volume daily and the water temperature and salinity averaged 27.0°C and 28.6 ppt, respectively. Daily photoperiod was maintained at 13 hours of light. Ovarian maturation, spawning, molting rate, and survival were monitored daily and growth and gonad size were measured at termination. The composite diet was the best overall diet, while squid was the best single-food diet, followed by shrimp, worms, and clams. No maturation of unablated females was observed with the exception of one female that developed to stage 3. Although the size of the shrimp was considered marginal for spawning, 5 mated females were observed, but only one (squid treatment) produced viable nauplii. After termination of this study, all shrimp were fed the composite diet for 41 days. During this period, ovarian maturation and spawning occurred in the previously unproductive treatment receiving only clams, and growth rate of shrimp in all previously single-food treatments increased 4 to 7 fold. The composite diet was also fed to two tanks of P. stylirostris (mean initial weight = 45.4 g) for a period of 118 days. During that time, 50 spawns were obtained with up to 3 spawns per female. A total of 4.3 × 105 P. stylirostris nauplii were produced. 相似文献
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海水比重、温度和底质对青蛤稚贝生长、存活的影响 总被引:7,自引:1,他引:7
本文阐述了海水比重、温度和底质对青蛤稚贝生长和存活率的影响。青蛤稚贝在1.010~1.025比重范围内,生活正常,比重越高,生长越快;超过1.025,比重越高,生长变慢,存活率降低。青蛤适宜生长和存活的温度范围为10~33℃,在此范围内,温度越高,生长越快,存活率也就越高;超过33℃时,生长变慢,存活率降低。青蛤稚贝适宜底质为砂质和细砂质,纯泥质不适青蛤稚贝生长。 相似文献
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光照对黄盖鲽仔鱼生长_发育及摄食的影响 总被引:12,自引:1,他引:12
光照对黄盖鲽仔鱼的生长、发育及摄食有显著影响,各组仔鱼的体长与体高在20日时均产生极显著的差异;Duncan多范围检验表明:40-60lx光照度下仔鱼生长最好;在40-7000lx范围内随光照度的增强,生长则变差,存活率下降,畸形个体增加,暗条件下及3-6lx条件下的仔鱼在12日龄时全部因饥饰物而死亡。在卵黄囊期的各组仔鱼不受外界环境影响,生长情况近似。40-7000lx光照度之间的5且在4日龄至 相似文献
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To examine the hypothesis that EDTA increases hatching rates and survival of penaeid shrimp larvae by decreasing the toxicities of heavy metals through chelation, the toxicity of cadmium, a highly toxic metal, was compared to the toxicities of calcium and phenol in both the presence and absence of EDTA. In addition, the toxicity of EDTA at higher concentrations was examined. The toxicities of EDTA, cadmium, calcium, and phenol were evaluated in terms of the percentage of nauplii surviving after 24 hours of exposure and the percentage of nauplii which metamorphosed to protozoea. The toxicities of cadmium, calcium, and phenol were also determined in the presence of EDTA. EDTA concentrations of 1.34 mM were lethal to nauplii. At 0.67 mM, EDTA reduced the percentage of nauplii which metamorphosed to protozoea but below 0.3 mM neither survival nor metamorphosis were affected. Cadmium, phenol, and calcium were lethal to nauplii at concentrations of 20 μM, 7 mM, and 400 mM, respectively. Metamorphosis was blocked by concentrations of 1 μM, 0.9 mM, and 200 mM, respectively. However, in the presence of 0.3 mM EDTA, the toxicities of cadmium and calcium were reduced. Cadmium concentrations of 20 μM did not affect either survival or metamorphosis in the presence of EDTA. In calcium concentrations of 50 and 100 mM, the percentage of nauplii that metamorphosed to protozoea was increased by the addition of EDTA. The interaction of EDTA and phenol toxicity was not significant. 相似文献
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对虾养殖池沉积环境中TOC_TP_TN和pH垂直分布 总被引:11,自引:1,他引:10
1995年冬季,测定了青岛地区四个池龄10年以上的对虾养殖池不同层次沉积物中有机碳(TOC)、总氮(TN)、总磷(TP)和pH并研究了其垂直分布。结果表明,沉积物中的TOC和TN污染较底层严重,泥质虾池表层污染较泥沙质更严重。沉积物中TP为输出过程而导致缺乏,TP的受扰动层与TOC和TN的污染层基本一致。沉积物的pH无规律性变化。 相似文献
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鱿鱼鱼精蛋白的提取_纯化及其生化特性 总被引:16,自引:0,他引:16
鱼精蛋白(Protamine)是一种常与DNA结合,存在于鱼类成熟精巢中的碱性蛋白。由于其生化组成的特点,鱼精蛋白不仅具有促使细胞发育繁殖的作用,而且一旦将其分离出来,能有效抑制多种食品腐败菌的生长和繁殖[Miler1942]。同时,这种鱼精蛋白还有... 相似文献