离子交换树脂法在土壤N素研究中的应用进展

概述了离子吸附树脂三种不同形式在土壤N素上的应用情况,指出了各种方法的优缺点,并对影响测定结果的因素进行了分析,同时提出了该技术在土壤N素研究中的应用前景。     

长期不同化肥氮用量对设施菜地土壤氮素矿化和硝化作用的影响

为揭示长期施用不同化肥氮对设施菜地土壤供氮能力的影响,选取连续种植15年不同化肥氮用量下的设施菜地土壤,采用好气密闭培养法研究长期不同化肥氮用量对设施菜地土壤氮素矿化和硝化作用的影响.供试土壤为5个氮施用水平,分别为:不施化肥氮(CK),常规化肥氮(100％N),常规化肥氮上减氮20％(80％N)、40％(60％N)、...     

菜地和旱作粮地土壤氮素矿化和硝化作用的比较

采用室内培养试验研究了南京郊区菜地和早作粮地土壤氮素矿化和硝化作用的特征,其菜地土壤是20年前从粮食作物改种为蔬菜的。结果表明,菜地土壤氮素矿化量和矿化率都显著高于相应的粮地土壤。改制对硝化作用没有固定的影响,其硝化作用是增强还是减弱主要取决于土壤pH是上升或下降。土壤氮素的矿化和硝化速率受土壤速效磷、速效钾、有机质含量和pH的影响。     

Evaluation of Si availability in slag fertilizers by an extraction method using a cation exchange resin

A new extraction method for the evaluation of Si availability in slag fertilizers was developed based on findings on the dissolution process of the slags in paddy fields. In the method, the slags were dissolved in water with the addition of a weakly acidic cation exchange resin (H form). The effects of the slag/water ratio, the amount of resin, and temperature on the Si dissolution from the slags were examined in order to determine adequate extraction conditions. The Si dissolution from the slags was enhanced by the addition of the resin. The pH of the extractant was well controlled between 6 and 7 during the extraction. The percentage of the amount of Si extracted by traditional evaluation methods using 0.5 м HCl or an acetate buffer solution to the total amount of Si in the slags was much higher than the Si recovery rate by rice plant (Oryza sativa L. var. Nihonbare) which was measured in our previous study. Moreover, there was no correlation between these values. On the other hand, the percentage of Si extracted by the new method was in the same range as that of the Si recovery rate and a positive correlation was obtained. As a result, Si availability in the slags could be evaluated more precisely by using the method proposed here than by using the traditional methods.     

生物炭和秸秆对华北农田表层土壤矿质氮和pH值的影响

基于2014-2015年华北农田定位试验,设CK（单施氮磷钾肥）、C1（生物炭4.5t×hm^-2×a^-1+氮磷钾肥）、C2（生物炭9.0t×hm^-2×a^-1+氮磷钾肥）和SR（秸秆还田+氮磷钾肥）4个处理,对施用生物炭和秸秆还田对表层土壤矿质氮（NO₃^--N、NH₄⁺-N）含量以及土壤pH值的影响进行研究。结果表明,不同处理土壤矿质氮的动态变化趋势基本一致,施用生物炭和秸秆还田均可显著提高土壤NO₃^--N含量（P<0.05）,但对土壤NH₄⁺-N含量影响不大。与秸秆还田相比,高量施用生物炭有利于增加土壤NO₃^--N含量。各处理土壤中矿质氮主要以NO₃^--N为主,NH₄⁺-N含量均保持在一个较低水平。将冬小麦整个生育期内各处理土壤NO₃^--N、NH₄⁺-N含量与夏玉米的相比,前者显著高于后者。在整个冬小麦-玉米轮作周期内,高量施用生物炭显著提高了土壤pH值,且各处理土壤NO₃^--N与土壤pH值呈显著负相关（P<0.05）,土壤NH₄⁺-N含量与土壤pH值相关性不显著;而各处理土壤NO₃^--N、NH₄⁺-N含量与土壤含水量均呈显著正相关（P<0.05）。可见,添加生物炭对减少氮素的转化和流失具有较大潜力。     

Initial recovery of soil carbon and nitrogen pools and dynamics following disturbance in jack pine forests: A comparison of wildfire and clearcut harvesting

Forests naturally maintained by stand-replacing wildfires are often managed with clearcut harvesting, yet we know little about how replacing wildfire with clearcutting affects soil processes and properties. We compared the initial recovery of carbon (C) and nitrogen (N) pools and dynamics following disturbance in jack pine (Pinus banksiana) stands in northern Lower Michigan, USA, by sampling soils (Oa+A horizons) from three “treatments”: 3-6-year-old harvest-regenerated stands, 3-6-year-old wildfire-regenerated stands and 40-55-year-old intact, mature stands (n=4 stands per treatment). We measured total C and N; microbial biomass and potentially mineralizable C and N; net nitrification; and gross rates of N mineralization and nitrification. Burned stands exhibited reduced soil N but not C, whereas clearcut and mature stands had similar quantities of soil organic matter. Both disturbance types reduced microbial biomass C compared to mature stands; however, microbial biomass N was reduced in burned stands but not in clearcut stands. The experimental C and N mineralization values were fit to a first-order rate equation to estimate potentially mineralizable pool size (C₀ and N₀) and rate parameters. Values for C₀ in burned and clearcut stands were approximately half that of the mature treatment, with no difference between disturbance types. In contrast, N₀ was lowest in the wildfire stands (170.2 μg N g⁻¹), intermediate in the clearcuts (215.4 μg N g⁻¹) and highest in the mature stands (244.6 μg N g⁻¹). The most pronounced difference between disturbance types was for net nitrification. These data were fit to a sigmoidal growth equation to estimate potential NO₃⁻ accumulation (Nit_max) and kinetic parameters. Values of Nit_max in clearcut soils exceeded that of wildfire and mature soils (149.2 vs. 83.5 vs. 96.5 μg NO₃⁻-N g⁻¹, respectively). Moreover, the clearcut treatment exhibited no lag period for net NO₃⁻ production, whereas the burned and mature treatments exhibited an approximate 8-week lag period before producing appreciable quantities of NO₃⁻. There were no differences between disturbances in gross rates of mineralization or nitrification; rather, lower NO₃⁻ immobilization rates in the clearcut soils, 0.20 μg NO₃⁻ g⁻¹ d⁻¹ compared to 0.65 in the burned soils, explained the difference in net nitrification. Because the mobility of NO₃⁻ and NH₄⁺ differs markedly in soil, our results suggest that differences in nitrification between wildfire and clearcutting could have important consequences for plant nutrition and leaching losses following disturbance.     

乙炔抑制方式对潮土硝化和矿化作用的影响

在土壤最大持水量60%和温度28?C的实验室培养条件下,研究了乙炔的不同抑制方式(短时前期暴露/连续灌注法)对潮土硝化、矿化作用的影响。结果显示,连续14天灌注10 ml/L或100 ml/L浓度乙炔完全抑制了供试潮土的硝化作用,而将土样短时前期暴露于乙炔12 h后驱散乙炔,仅能保持48 h的抑制效果,驱散乙炔后第3天土样的硝化作用开始恢复,培养结束时土样的硝化率仍可达到99%。本研究的结果还显示,培养结束时,加入乙炔的4个施N处理其净矿化量为负值,乙炔连续抑制方式下土样净矿化量低于乙炔短时前期暴露方式下土壤的净矿化量。因此,采用乙炔抑制技术进行研究时应当考察所采集的供试土壤在乙炔抑制方式下的硝化活性恢复速率和矿化过程,以保证相关试验方法设计的合理性。对培养期较长的试验,采用连续灌注乙炔,并将通风时间控制在硝化活性恢复点以前的方式是区分硝化类型较为适用的实验室方法。     

Changes in soil nitrogen availability due to stand development and management practices on semi‐arid sandy lands,in northern China

Soil nitrogen (N) availability is one of the limiting factors for plant growth on sandy lands. Little is known about impacts of afforestation on soil N availability and its components in southeastern Keerqin sandy lands, China. In this study, we measured N transformation under sandy Mongolian pine (Pinus sylvestris var. mongolica Litv.) plantations of different ages (grassland, young, middle‐aged, close‐to‐mature) and management practices (non‐grazing and free‐grazing) during the growing seasons using the ion exchange resin bag method. Results showed that, for all plots and growing season, soil NH‐N, NO‐N, mineral N, and relative nitrification index, varied from 0·18 to 1·54, 0·96 to 22·05, 1·23 to 23·58 µg d⁻¹ g⁻¹ dry resin, and 0·76 to 0·97, respectively, and NO‐N dominated the available N amount due to intense nitrification in these ecosystems. In general, the four indices significantly increased in the oldest plantation, with corresponding values in non‐grazing sites lower than those in free‐grazing sites (p < 0·05). Our studies indicated that it is a slow, extended process to achieve improvement in soil quality after afforestation of Mongolian pine in the study area. Copyright © 2009 John Wiley & Sons, Ltd.     

慈溪1000年时间序列农田土壤氮矿化和硝化的变化

长时间序列土壤关键氮（N）转化过程和相关微生物的变化规律仍不清楚。以慈溪市滨海围垦农田土壤为研究对象,利用室内培养法研究了11个不同利用年限土壤（0～1 000 a）N矿化速率、硝化强度和硝化细菌数量。结果表明,随着利用年限的增加,土壤电导率和pH下降,而有机质和全氮逐渐积累,土壤性质在利用前50 a内变化幅度较快;土壤N矿化速率的大小表现为：220～1 000 a >0～50 a >60～200 a;硝化强度大体随着利用年限的延长而增强,而土壤硝化细菌数量表现为在0～20 a增加后逐渐降低的趋势,其中,20～60 a达到最高;皮尔森相关和聚类推进树（ABT）分析表明,土壤利用年限（45%）、电导率（12%）和有机质（11%）是影响硝化强度的主控因子,土壤中NH₄⁺-N和有效磷含量分别是影响N矿化速率（86%）和硝化细菌数量（42%）的关键限制因子。因此,海陆界面土壤在持续农业利用过程中,硝化强度及硝化细菌丰度得到一定加强,但同时受到历史条件和当代环境因素的共同影响。     

闽南农业流域土壤氮矿化作用初步研究

用原位培养法对五川流域内的香蕉地、菜地、香蕉地与菜地间作地、林地、甘蔗地土壤的氮矿化作用进行了研究,结果发现不同土地利用方式下土壤氮矿化速率平均为N0.11mgkg-1d-1,其中香蕉地土壤氮矿化最高可达N1.47mgkg-1d-1;土壤硝化速率平均为N0.27mgkg-1d-1,其中菜地下土壤硝化作用强度最高可达N0.69mgkg-1d-1。土壤矿化作用同土壤湿度、温度、C/N、土壤肥力存在明显的相关关系。不同土地利用方式下温度是土壤氮矿化最主要的影响因子。闽南流域土壤年矿化量平均为N240kghm-2,部分样地最高可达N850kghm-2,而年施肥量平均N650kghm-2。相比较而言,说明了土壤矿化是我国南方流域农业土壤氮素的重要来源。     

国产和进口阴离子交换树脂膜埋置法测定土壤中的有效磷

对采自全国各地 2 0个土壤样品进行测定 .结果表明 ,树脂膜法测定的土壤磷值与碳酸氢钠法测定的土壤有效磷和植物实际吸磷量间具有极显著的相关性 ,国产树脂膜可代替进口树脂膜用于大田土壤有效磷测定     

Field simulation of wet and dry years in the Chihuahuan desert: soil moisture,N mineralization and ion-exchange resin bags

Irrigation and rain-out shelters were used to simulate precipitation patterns of wet and dry years in the northern Chihuahuan Desert. Irrigation provided approximately double the long-term average monthly precipitation. Rain was excluded during the wet season, July-October, to simulate a dry year. N net mineralization in laboratory incubations was undectable at calculated water potentials less than -1 MPa. Witb increasing moisture, mineralization gradually rose to the highest observed rates near field capacity. There was no mineralization maximum at moisture contents below field capacity. Irrigation significantly increased the water potential and rainfall exclusion reduced water potentials to less than-8 MPa. The general absence of important irrigation effects may have resulted from the high natural precipitation during the experiment or because irrigation inputs were insufficient to increase microbial activity during very dry periods. Precipitation exclusion reduced ion capture during the warm-wet season. After allowing precipitation inputs to resume, NH ₄ ⁺ -N capture was increased in the cool-dry seasons of both 1987–1988 and 1988–1989. NH ₄ ⁺ -N capture more than doubled that predicted from the overall covariance of moisture input and ion capture, suggesting increased availability of N. An unusually hot, dry period in May and June 1989 was followed by a threeto fourfold increase in the warm-wet season NO ₃ ^– +NO₂–N capture compared to 1988. These data suggest that short droughts of about 3 months in length (both simulated and natural) increased N availability relative to moisture availability.     

Soil microbial biomass, activity and potential nitrogen mineralization in a pasture: Impact of stock camping activity

Grazing animals recycle a large fraction of ingested C and N within a pasture ecosystem, but the redistribution of C and N via animal excreta is often heterogeneous, being highest in stock camping areas, i.e., near shade and watering sources. This non-uniform distribution of animal excreta may modify soil physical and chemical attributes, and likely affect microbial community eco-physiology and soil N cycling. We determined microbial population size, activity, N mineralization, and nitrification in areas of a pasture with different intensity of animal excretal deposits (i.e., stock camping, open grazing and non-grazing areas). The pasture was cropped with coastal bermudagrass (Cynodon dactylon L.) and subjected to grazing by cattle for 4 y. Soil microbial biomass, activity and N transformations were significantly higher at 0-5 cm than at 5-15 cm soil depth, and the impacts of heterogeneous distribution of animal excreta were more pronounced in the uppermost soil layer. Microbial biomass, activity and potential net N mineralization were greater in stock camping areas and were significantly correlated (r²≈0.50, P<0.05) with the associated changes in total soil C and N. However, gross N mineralization and nitrification potential tended to be lower in stock camping areas than in the open grazing areas. The lower gross N mineralization, combined with greater net N mineralization in stock camping areas, implied that microbial N immobilization was lower in those areas than in the other areas. This negative association between microbial N immobilization and soil C is inconsistent with a bulk of publications showing that microbial N immobilization was positively related to the amount of soil C. We hypothesized that the negative correlation was due to microbial direct utilization of soluble organic N and/or changes in microbial community composition towards active fungi dominance in stock camping areas.     

Microbial biomass and microbial C:N ratio in bulk soil and buried bags for evaluating in situ net N mineralization in agricultural soils

The in situ net nitrogen mineralization (N_net) was estimated in five agricultural soils under different durations of organic farming by incubating soil samples in buried bags. Simultaneously, soil microbial C and N was determined in buried bags and in bulk soil under winter wheat and after harvest. The aim was to check for variations in soil microbial biomass contents and microbial C:N ratios during the incubation period, and their importance for N_net rates. Microbial C and N contents were highest in soils that had been organically farmed for 41 years, whereas N_net rates were highest in a short‐term organically managed soil that had been under grassland use until 36 years ago. The mean coefficient of variation in the bulk soil for microbial C estimates ranged from 5 to 12 %. Microbial N contents were similar inside buried bags and in the bulk soil at the end of the incubation periods. Under winter wheat during the incubation period until harvest, microbial C contents and microbial C:N ratios (in 10—27 cm depth only) decreased more strongly inside buried bags than in the bulk soil. Following harvest of winter wheat and ploughing, microbial biomass increased while in situ N_net decreased, presumably due to N immobilization. The N_net rates were not correlated with microbial N contents or changes in microbial N contents inside buried bags. At the end of the vegetation period of winter wheat, N_net rates were negatively correlated with microbial C:N ratios. Because these ratios concurrently decreased more inside buried bags than in the bulk soil, the N_net estimates of the buried bag method may differ from the N_net rates in the bulk soil at that time.     

Neglect of mowing and manuring leads to slower nitrogen cycling in subalpine grasslands

Nitrogen (N) availability in grasslands varies with agricultural land use. Traditional management regimes of mowing for hay and manuring in subalpine meadows maintain plant communities with exploitative functional strategies suited to fertile soils with fast turnover of nutrients. We investigated whether the neglect of traditional practices has led to a reduction in N availability in two parallel ecosystems (terraced and unterraced fields). Soil nitrate and ammonium contents were assessed using soil cores and ion exchange resins over a 1-year period, and assays of microbial nitrifying and denitrifying enzyme activities, made early in the growing season. A large difference in pH between the two ecosystems, caused by historical ploughing, facilitated greater N availability in terraced than unterraced fields. Abandonment of manuring and mowing caused a reduction in N availability and N transformation processes, which correlated with a shift in the plant community towards more-conservative functional strategies and greater dominance by grasses. We propose that positive feedback between the grassland management regime and dominant plant functional strategy maintained high N availability in these managed subalpine grasslands. When traditional practices of mowing and manuring are neglected, direct management effects combined with the spread of grass species with conservative strategies force down N availability in the soil, reduce microbial activity, change the pH, and lead to a long-term loss of characteristic herbaceous subalpine-meadow species.     

Effects of litter (beech and stinging nettle) and earthworms (Octolasion lacteum) on carbon and nutrient cycling in beech forests on a basalt-limestone gradient: A laboratory experiment

Effects of leaf litter of beech (Fagus sylvatica L.) and stinging nettles (Urtica dioica L.) and of the endogeic earthworm species Octolasion lacteum (Örley) on carbon turnover and nutrient dynamics in soil of three beechwood sites on a basalt hill (Hesse, Germany) were investigated in a laboratory experiment lasting for about 1 year. The sites were located along a gradient from basalt (upper part of the hill) to limestone (lower part of the hill) with an intermediate site in between (transition zone). At the intermediate site U. dioica dominated in the understory whereas at the other sites Mercurialis perennis L. was most abundant. The amount and composition of organic matter was similar in soil of the basalt (carbon content 5.9%, C/N ratio 13.8) and intermediate site (carbon content 5.6%, C/N ratio 14.3) but the soil of the intermediate site produced more CO₂ (in total +17.5%) and more nitrogen (as nitrate) was leached from this soil (in total +55.6%). It is concluded that the soil of the intermediate site contains a large mobile carbon and nitrogen pool and the formation of this pool is ascribed to the input of U. dioica litter. Leaf litter of U. dioica strongly increased NO₃ ^–-N leaching immediately after the litter had been added, whereas nitrogen was immobilized due to addition of beech litter. Despite the very fast initial decomposition of nettle litter, the increase in CO₂ production due to this litter material was only equivalent to 20.1% of the amount of carbon added with the nettle litter; the respective value for beech litter was 34.8%. Earthworms altered the time course of carbon and nitrogen mineralization in each of the treatments. In general, earthworms strongly increased mineralization of nitrogen but this effect was less pronounced in soil of the intermediate site (treatments without litter), which is ascribed to a depleted physically protected nitrogen and carbon pool. In contrast, their effect on the total amount of nitrogen mobilized from nettle litter was small. Earthworms significantly reduced CO₂ production from soil of the intermediate site (treatments without litter) and it is concluded that earthworm activity contributes to the restoration of the depleted physically protected carbon pool at this site.     

Modelling runoff and soil erosion in logged forests: Scope and application of some existing models

Predictive erosion models are useful tools for evaluating the impact of land-use practices on soil and water properties, and as often used by environmental protection authorities, for setting guidelines and standards for regulation purposes. This study examines the application of three erosion models of varying complexity and design for predicting runoff and soil erosion from logged forest compartments in south eastern Australia. These are: the Universal Soil Loss Equation (USLE), the Water Erosion Prediction Project (WEPP), and TOPOG, a physically based hydrologic modelling package. Data on rates of soil loss and redistribution collected during a series of large-scale rainfall simulator experiments were used as model input parameters and validation. The models were evaluated in terms of general ease of use, input data requirements and accuracy of process understanding and prediction. Results suggest that in this application the USLE overestimated soil loss, and have the limitation that it does not predict sediment yield or sediment redistribution for specific storm events. When used at the hillslope scale, WEPP and TOPOG have predicted runoff and soil loss reasonably well, particularly on disturbed surfaces such as skid trails. On less disturbed surfaces such as the general harvesting area, both models performed less accurately, generally under-predicting soil loss and sediment yield, notably on sites with low observed values. The complexity and data requirements of WEPP and TOPOG limit their usability as a general-purpose, erosion hazard predicting tool. In terms of process understanding, none of the existing models accurately depict the nature and extent of sediment redistribution quantified in the rainfall simulator experiments. In order to advance the application and accuracy of modelling tools in forestry environments, this redistribution process should be considered integral to the refinement and redevelopment of future models.     

Role of nitrite and nitric oxide in the processes of nitrification and denitrification in soil: Results from N tracer experiments

Recent research has proven soil nitrite to be a key element in understanding N-gas production (NO, N₂O, N₂) in soils. NO is widely accepted to be an obligatory intermediate of N₂O formation in the denitrification pathway. However, studies with native soils could not confirm NO as a N₂O precursor, and field experiments mainly revealed ammonium nitrification as the source of NO. The hypothesis was constructed, that the limited diffusion of NO in soil is the reason for this contradiction. To test this diffusion limitation hypothesis and to verify nitrite and NO as free intermediates in native soils we conducted through-flow (He/O₂ atmosphere) ¹⁵N tracer experiments using black earth soil in an experimental set up free of diffusion limitation. All of the three relevant inorganic N soil pools (ammonium, nitrite, nitrate) were ¹⁵N labelled in separate incubation experiments lasting 81 h based on the kinetic isotope method. During the experiments the partial pressure of O₂ was decreased in four steps from 20% to about 0%. The net NO emission increased up to 3.7 μg N kg⁻¹ h⁻¹ with decreasing O₂ partial pressure. Due to the special experimental set up with little to no obstructions of gas diffusion, only very low N₂O emission could be observed. As expected the content of the substrates ammonium, nitrate and nitrite remained almost constant over the incubation time. The ¹⁵N abundance of nitrite revealed high turnover rates. The contribution of nitrification of ammonium to the total nitrite production was approx. 88% under strong aerobic soil conditions but quickly decreased to zero with declining O₂ partial pressure. It is remarkable that already under the high partial pressure of 20% O₂ 12 % of nitrite is generated by nitrate denitrification, and under strict anaerobic conditions it increases to 100%. Nitrite is present in two separate endogenous pools at least, each one fed by the nitrification of ammonium or the denitrification of nitrate. The experiments clearly revealed that nitrite is almost 100% the direct precursor of NO formation under anaerobic as well as aerobic conditions. Emitted N₂O only originated to about 100% from NO under strict anaerobic conditions (0-0.2% O₂), providing evidence that NO is a free intermediate of N₂O formation by denitrification. To the best of our knowledge this is the first time that NO has been detected in a native soil as a free intermediate product of N₂O formation at denitrification. These results clearly verify the “diffusion limitation” hypothesis.     

Nitrogen fixation and CO2 exchange in soybeans (Glycine max L.) inoculated with mixed cultures of different microorganisms

N₂ fixation, photosynthesis of whole plants and yield increases in soybeans inoculated with mixed cultures of Bradyrhizobium japonicum 110 and Pseudomonas fluorescens 20 or P. fluorescens 21 as well as Glomus mosseae were found in pot experiments in gray forest soil carried out in a growth chamber. The effects of pseudomonads and vesicular-arbuscular (VA) mycorrhizal fungus on these parameters were found to be the same. Dual inoculation of soybeans with mixed cultures of microorganisms stimulated nodulation, nitrogenase activity of nodules and enhanced the amount of biological nitrogen in plants as determined by the ¹⁵N dilution method in comparison to soybeans inoculated with nodule bacteria alone. An increased leaf area in dually infected soybeans was estimated to be the major factor increasing photosynthesis. P. fluorescens and G. mosseae stimulated plant growth, photosynthesis and nodulation probably due to the production of plant growth-promoting substances. Increasing phosphorus fertilizer rates within the range of 5–40 mg P 100 g^-1 1:1 (v/v) soil: sand in a greenhouse experiment led to a subsequent improvement in nodulation, and an enhancement of N₂ fixation and yield in soybeans dually inoculated with B. japonicum 110 and P. fluorescens 21. These indexes were considerably higher in P-treated plants inoculated with mixed bacterial culture than in plants inoculated with nodule bacteria alone.