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1.
应用锥板式粘度计和红细胞电泳仪对31头健康黄牛血液流变学7项指标进行测定。结果:全血粘度0.2cm=s·3.09±3.39mPa·s,0.5cm/s时3.66±3.29mPa·s,2cm/s时4.88±3.35mPa·s,5cm/s时5.38±3.17mPa·s,20cm/s时5.51±2.12mPa·s,50cm/s时4.84±2.30mPa,100cm/s时3.42±0.97mPa·s;全血比  相似文献   

2.
本文提出了饲料中钙的测定的新方法,试验表明,在pH10.5~11.5的氨-氯化铵缓冲介质中,钙与三溴偶氮胂形成稳定的紫色配合物,在波长605nm处有最大吸收,表现摩尔吸光系数ε_(605)=2.3×10 ̄4L·mol ̄(-1)·cm ̄(-1),检测的线性范围为5~65μg/50ml。进一步利用双波长光度法可使方法灵敏度提高到ε_(490~605)=3.5×10 ̄4L·mol ̄(-1)·cm ̄(-1)。本方法用于饲料样品的测定,结果与国家标准法相符,并明显优于EDTA配位滴定法。  相似文献   

3.
猪核移植重组胚胎的发育能力   总被引:1,自引:0,他引:1  
以McGrath-Solter(1983)提供的去(注)核方法,将猪胚胎的单个卵裂球细胞注入去核的次级成熟卵母细胞,借助电融合的方法(AC;5V,1.5MHz,12-15s;DC:2kV/cm,40μs。电激活/融合液:0.3mol/L甘露醇+-.1mmol/LMgSO4+0.1mmolCacL2+7.5mg/mlCCB)融入受体胞质构成重级胚,体外培养(培养液为改衣TCM-199;培养条件为5%  相似文献   

4.
家蚕RAPD的扩增条件、重复性及遗传模型研究   总被引:36,自引:15,他引:21  
发现当PCR体系中Mg ̄(2+)为2mmol/L、dNTPs为150μmol/L、Tag酶为1U、引物为0.2μmol/L时,以40~42℃退火可得到稳定结果;8%的DMSO对提高扩增特异性有利;分别从蚕卵、蚁蚕、5龄蚕丝腺、体壁及生殖腺、蛹、蛾中提取模板DNA,其RAPD结果一致;以C_(108)×大造为材料,发现其F_1RAPD为共显性。  相似文献   

5.
台湾芒草之光合作用特性   总被引:3,自引:0,他引:3  
翁仁宪 《中国草地》1994,(1):14-25,34
芒草为C4型植物,叶片上表皮气孔数目很少,高山所产者尤甚,而且产于高山的芒草,其叶片上表皮覆盖一层可能是角质层的物质,不容易分辨表皮细胞,而产于低海拔者则否。其CO2补偿点,除了高山芒接近9ppm之外,其余在3.5~6.5ppm。光合成率对光度之反应呈饱和曲线。在未施氮区,芒草单叶之饱和点为750~1250μE/m^2/s,其最光合(Pn介于8-16μmolCO2/m^2/s之间,每盆施氮2g,1  相似文献   

6.
对106头青海黑牦牛的血清钙分析结果表明:①STCa、SBCa和SFCa%分别为2.55±0.22m mol/L、0.97±0.25m mol/L、1.60±0.16mmol/L和37.22%。②STCa和SFCa间,STP和SBCa间,均呈极显著正相关(P〈0.01),回归方程分别为y=-1.2561+0.8719x。  相似文献   

7.
我们于1996年4 ̄6月对笼养10 ̄12月龄种用乌骨鸡进行人工授精技术研究。其结果:乌骨鸡精液颜色为乳白色,射精量为0.49±0.238ml;精子密度20.3±10.84×10^8/ml;精子的畸形率(5.79±1.64)%。乌鸡骨精子形态与家鸽同,精子总长度为(80.59±3.94)μm,头为弯园柱状,直径0.5μm,头长约8μm,精子头部前端为顶林,长约2μm,尾部(中、主、末段)长71±2.  相似文献   

8.
从桑尺蠖(HemerophilaatrilineataButler)中分离的微孢子虫(简称桑尺蠖微孢子虫),形态为长卵圆形,大小为3.5~4.1×1.6~1.9μm。从丝棉木金星尺蠖(CalospilossuspectaWarren)中分离的微孢子虫(简称丝棉木金星尺蠖微孢子虫),形状为卵圆形,大小为3.2~3.7×1.6~2.1μm。两种微孢子虫均能感染寄生家蚕的主要组织器官,引起蚕儿发病,但致病力要比家蚕微孢子虫(Nosemabombycis)弱,且能经卵传染给下一代,其胚种传染率要比家蚕微孢子虫低。  相似文献   

9.
对耳部感染兔痒螨的家兔采取400μg/kg剂量的双氢除虫菌素皮下注射,其临床分级显著降低。对由TrixacaurusCuvlae引起螨病的豚鼠以500μg/kg双氢除虫菌素皮下注射,得到临床治愈。家兔皮下注射400μg/kg双氢除虫菌素,组织和体液中产生高而持久的浓度至少达13天。其在组织中衰减速率与在绵羊和大鼠中观察到的情况相似。在用药37.2±5.0小时血浆中的平均(±平均标准误)峰值浓度为42.0±9.ng/ml。药时曲线下面积(AUC)为3543±580μg/ml·h,给豚鼠口服、皮下注射或局部用500μg/kg剂量的双氢除虫菌素,只有皮下注射能测出血浆中药物浓度。在用药后72小时,四只豚鼠的平均浓度为0.7±0.3ng/ml。  相似文献   

10.
应用电灼烧和强酸腐蚀的方法成功地对10 周龄公雏鸡实行了肾上腺去除术。在此基础上对完全去肾上腺雏鸡给以外源性糖皮质激素( G C)地塞米松( Dex)2 m g/kg·d可以逆转由于 G C 缺失造成的血糖降低和水盐代谢紊乱。对去除1 23肾上腺雏鸡并给以 R U486 P V A 50 m g/kg·d,雏鸡血糖、血清钠,血清钾维持正常水平;虽然其血清 A C T H 浓度升高至(146. 94±7.56) I U/ L( P< 0.01),但血清皮质酮并没有在高浓度 A C T H 刺激下大量分泌而维持于90.48~103.14 nm ol/ L 的生理范围内。为在不受内源性 G C干扰下建立鸡糖皮质激素受体( G R)阻断提供了实验动物模型。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
15.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

16.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

17.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

18.
19.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

20.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

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