Photochemical Degradation of Phenols in Water Using Flavin-based Photosensitizers

A slightly modified method for 10-ethyl flavin was developed in the present study.The synthetic product was characterized by nuclear magnetic resonance (NMR) and mass spectrometry,and used to catalyze the photocatalytic degradation of phenol,2,4-dichlorophenoxyacetic acid,p-nitrophenol,4-chlorophenol,4-methoxyphenol,4-chloro-2-methyl-phenoxyacetic acid and 2,4,5-trichloro-phenoxyacetic acid.Both HPLC (high performance liquid chromatography) and GC-MS data suggested that all phenols were degraded in the presence of either flavin at micromolar concentrations under direct sun light.A rapid breakdown of the phenols was observed.The degradation efficiency was clearly dependent on phenol type.In a decreasing order of degradation efficiency over a 2-h period,the phenols were 4-chlorophenol and 4-methoxyphenol (-80％) ＞ phenoxyacetic acids (60％-65％) ＞ nitrophenol and phenol (-35％).     

EDTA对镉胁迫下紫云英种子萌发及幼苗生理抗性的影响

采用水培法研究不同浓度EDTA(乙二胺四乙酸)对镉胁迫下紫云英(Astragalus sinicus L.)种子萌发及幼苗生理抗性的影响,为重金属污染植物复合修复提供科学依据。结果表明:单一的镉(浓度为100 mg/L)胁迫紫云英时,能促进种子萌发,增加幼苗中丙二醛(MDA)的含量以及过氧化物酶(POD)的活性,但是会抑制幼苗的生长、降低幼苗的叶绿素含量以及超氧化物歧化酶(SOD)的活性。当在镉溶液中添加不同浓度的EDTA时,随着EDTA浓度的增加,其种子发芽率、幼苗长势、叶绿体含量以及POD活性都呈现出先增加后降低的趋势。就供试材料而言,在一定浓度范围内的EDTA能缓解镉胁迫紫云英幼苗的伤害作用,其中以0.05 mmol/L的EDTA处理效果最为明显。     

Chemical loss of ozone in the arctic polar vortex in the winter of 1991-1992

In situ measurements of chlorine monoxide, bromine monoxide, and ozone are extrapolated globally, with the use of meteorological tracers, to infer the loss rates for ozone in the Arctic lower stratosphere during the Airborne Arctic Stratospheric Expedition II (AASE II) in the winter of 1991-1992. The analysis indicates removal of 15 to 20 percent of ambient ozone because of elevated concentrations of chlorine monoxide and bromine monoxide. Observations during AASE II define rates of removal of chlorine monoxide attributable to reaction with nitrogen dioxide (produced by photolysis of nitric acid) and to production of hydrochloric acid. Ozone loss ceased in March as concentrations of chlorine monoxide declined. Ozone losses could approach 50 percent if regeneration of nitrogen dioxide were inhibited by irreversible removal of nitrogen oxides (denitrification), as presently observed in the Antarctic, or without denitrification if inorganic chlorine concentrations were to double.     

3种螯合剂对铅胁迫下香樟幼苗生长及铅富集的影响

为了研究在添加不同螯合剂的情况下,植物对土壤Pb(铅)胁迫的生理生长反应及对Pb的富集转运情况,揭示其变化机制,筛选出较好的螯合剂及适宜的质量摩尔浓度,选择香樟幼苗为试验对象,在Pb质量分数为1045 mg·kg^-1土壤中分别添加柠檬酸、苹果酸和乙二胺四乙酸(EDTA)3种螯合剂,每种螯合剂分别设置了0、2、4、6、8 mmol·kg^-15个质量摩尔浓度,测量香樟幼苗的生长指标、生理指标以及Pb富集转运等指标并进行统计分析。结果表明:3种螯合剂对香樟幼苗生长生理及Pb的富集转运均有显著影响,随着3种螯合剂质量摩尔浓度的增加,Pb胁迫加重;同一质量摩尔浓度下,多数指标,经EDTA处理的分别与经柠檬酸和苹果酸处理的结果有显著差异,柠檬酸和苹果酸处理二者之间差异不显著;EDTA质量摩尔浓度不大于2 mmol·kg^-1,柠檬酸、苹果酸质量摩尔浓度不大于4 mmol·kg^-1,香樟生长状况及Pb吸收综合效果最好。综合分析得出:柠檬酸、苹果酸、EDTA都具有促进植物吸收土壤Pb的作用,3种螯合剂均能提高香樟幼苗的富集系数和转移系数;当土壤Pb质量分数为1045 mg·kg^-1、EDTA质量摩尔浓度为2 mmol·kg^-1、柠檬酸和苹果酸质量摩尔浓度为4 mmol·kg^-1时,香樟幼苗吸收土壤Pb的效果最佳。在达到同等效率的前提下,建议优先选用柠檬酸或苹果酸为宜。     

荔枝果肉过氧化物酶酶学性质研究

本文从荔枝果肉中提取了过氧化物酶(POD),对其酶学性质进行了研究.结果表明,该酶最适反应温度为45℃,对热较敏感,当温度为70℃以上时,短时处理即可使酶活降至50％以下.最适pH 5,但在pH6.0～8.0范围内活力比较稳定.该酶在25℃和0.1 mol/L磷酸缓冲液(pH7.0)条件下对愈创木酚和没食子酸的Km值分别是4.64× 10-3和0.063 mol/L.谷胱甘肽和亚硫酸盐能完全抑制POD活性,但谷胱甘肽抑制浓度远低于亚硫酸盐,抗坏血酸只能在廷迟时间内抑制POD活性,对POD抑制具有暂时性,各抑制机制不同;CuSO4 、ZnSO4 、CaCl2 、MgCl2、EDTA对荔枝果肉POD活性有一定激活作用;FeSO4和MnSO4对POD活性有较强的抑制作用.     

皂苷与EDTA复合淋洗污染塿土中Cu、Pb的效果研究

采用振荡和柱淋洗方法,选用生物表面活性剂皂苷和螯合剂乙二胺四乙酸二钠盐(EDTA)作淋洗剂,研究了EDTA、皂苷及其复合液对污染塿土中重金属Cu、Pb的淋洗效果,并比较不同淋洗剂淋洗前后重金属的形态变化特征。结果表明:EDTA浓度为0.5、1.0 mmol·L-1时与不同质量浓度皂苷复合振荡淋洗Cu、Pb时表现为协同作用,对Pb的协同作用尤为明显;EDTA浓度大于5.0mmol·L-1时,与不同质量浓度皂苷复合淋洗Cu、Pb表现为拮抗作用。1.0 mmol·L-1EDTA、50 g·L-1皂苷及其复合淋洗液(1.0 mmol·L-1EDTA与50 g·L-1EDTA)对土柱中Cu、Pb的累积淋洗量均随着淋洗液体积的增大而逐渐增加;1.0 mmol·L-1 EDTA与50 g·L-1皂苷复合淋洗土柱中Cu时表现为协同作用,其对单一及复合污染塿土中Cu的累积淋洗百分率分别为23.59%和21.57%,对Pb的累积淋洗百分率分别为27.59%和24.92%。柱淋洗前后重金属形态分级结果表明,EDTA、皂苷及其复合淋洗液能有效去除碳酸盐结合态、有机结合态和硫化物残渣态重金属Cu、Pb;EDTA与皂苷及其复合淋洗液可显著活化污染塿土中的重金属Cu、Pb,有望与植物修复技术联用修复重金属污染塿土。     

Mechanisms of nitrogen dioxide reactions: initiation of lipid peroxidation and the production of nitrous Acid

The reactions of nitrogen dioxide with cyclohexene have been studied as a model for the reactions that occur between nitrogen dioxide in smoggy air and unsaturated fatty acids in pulmonary lipids. As predicted from earlier studies at high nitrogen dioxide concentrations, this gas reacts with cyclohexene predominantly by addition to the double bond at nitrogen dioxide concentrations of 1 percent (10,000 parts per million) to 40 percent in nitrogen; in the presence of air or oxygen, this reaction initiates the autoxidation of the alkene. However, at concentrations below 100 parts per million in nitrogen, nitrogen dioxide reacts with cyclohexene almost exclusively by abstraction of allylic hydrogen; this unexpected reaction also initiates the autoxidation of the alkene in the presence of oxygen or air, but it leads to the production of nitrous acid rather than of a product containing a nitro group attached to a carbon atom. The nitrous acid can react with amines to produce nitrosamines. Moreover, the nitrite ion produced by the hydrogen abstraction mechanism would be expected to diffuse throughout the body, unlike nitrated lipids that would be confined to the pulmonary cavity. These findings have been confirmed with methyl oleate, linoleate, and linolenate; some of the kinetic features of the nitrogen dioxide-initiated autoxidation of these unsaturated fatty acids have been studied.     

普鲁兰短梗霉N2-3菌株碱性蛋白酶的纯化及性质研究

[目的]对普鲁兰短梗霉N2-3菌株的碱性蛋白酶进行纯化,并对其性质进行研究。[方法]利用层析柱Sephadex G-75与阴离子交换柱DEAE Sepharose Fast Flow纯化菌株N2-3的胞外碱性蛋白酶,并对纯化的酶进行SDS-PAGE凝胶电泳检测,同时对纯化蛋白酶的最适反应温度、温度稳定性、最适反应pH、pH稳定性进行测定,研究金属离子及各种化合物对蛋白酶活性的影响。[结果]试验中N2-3菌株的碱性蛋白酶纯化倍数和回收率分别为2.34和25.9%;SDS-PAGE凝胶电泳显示该酶的分子量约为33 kDa;该酶的最适反应温度和pH分别为52℃和9.0;Mn2+、Mg2+、Na+离子浓度在5 mmol/L时对纯化蛋白酶的酶活有激活作用,Zn2+、Ca2+、K+、Li+对酶活的影响不大,而当存在Fe2+、Fe3+、Cu2+、Co2+、Ag+、Hg2+离子时,蛋白酶活明显降低;苯甲基硫酰氟(PMSF)强烈抑制了该蛋白酶的活性,而乙二胺四乙酸(EDTA)与碘乙酸微弱抑制了该蛋白酶的活性。[结论]为普鲁兰短梗霉N2-3菌株碱性蛋白酶的工业化生产提供了一定的理论基础。     

Effects of inhibitors on the protease profiles and degradation of activated Cry toxins in larval midgut juices of Cnaphalocrocis medinalis(Lepidoptera: Pyralidae)

Midgut juice plays an important role in food digestion and detoxification in insects. In order to understand the potential of midgut juice of Cnaphalocrocis medinalis(Guenée) to degrade Bt proteins, the enzymatic activity of midgut juice and its degradation of Bt proteins(Cry2 A, Cry1 C, Cry1 Aa, and Cry1 Ac) were evaluated in this study through protease inhibitor treatments. The activities of total protease in midgut juices were significantly inhibited by phenylmethylsulfonyl fluoride(PMSF), tosyl-L-lysine chloromethyl ketone(TLCK), pepstatin A and leupeptin. The enzymatic activity of chymotrypsin was significantly inhibited by PMSF, and enzymatic activity of trypsin was significantly inhibited by ethylenediaminetetraacetic acid(EDTA), PMSF, tosyl phenylalanine chloromethyl ketone(TPCK), TLCK and trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane(E-64). EDTA could significantly inhibit the degradation of Cry2 A by C. medinalis. EDTA, PMSF, TPCK, and TLCK could inhibit the degradation of Cry1 C and Cry1 Aa. EDTA, PMSF, TPCK, TLCK, and E-64 could inhibit the degradation of Cry1 Ac. Our results indicated that some protease inhibitors hindered various enzymatic activities in the larval midgut of C. medinalis, which may reduce the insect's ability to degrade Bt toxins. These findings may aid the application of protease inhibitors in the management of this insect pest in the future.     

Significance of tissue myo-inositol concentrations in metabolic regulation in nerve

Approximately 25 percent of resting energy utilization in isolated nerve endoneurium is inhibited by medium containing defatted albumin and selectively restored by arachidonic acid but is unaffected by indomethacin or nordihydroguaiaretic acid. The same component of energy utilization is inhibited by small decreases in endoneurial myo-inositol, which decrease incorporation of carbon-14-labeled arachidonic acid into phosphatidylinositol. The fraction of the resting oxygen uptake inhibited by ouabain is decreased 40 to 50 percent by a reduced tissue myo-inositol concentration or by defatted albumin. Metabolic regulation by rapid, basal phosphatidylinositol turnover is dependent on the maintenance of normal tissue myoinositol concentrations.     

螯合剂和有机酸对商陆修复镉砷污染农田的影响

基于利用植物提高Cd和As复合污染农田修复效率的目标，通过大田试验方法探究了乙二胺四乙酸（EDTA）、皂素（SAP）、柠檬酸（CA）和苹果酸（MA）对商陆萃取农田土壤Cd和As的影响。结果表明，施用4种不同螯合剂和有机酸对商陆的生长没有产生不利影响，且可以显著影响商陆对土壤Cd和As的吸收积累。与对照组相比，EDTA、SAP、CA和MA处理60 d后导致商陆叶片Cd含量分别提高116.4%、55.0%、81.5%和109.9%，商陆地上部Cd积累量提高40.4%~106.0%，商陆地上部As积累量提高15.8%~55.1%。商陆各器官Cd和As的富集系数和转运系数也受螯合剂和有机酸施用的影响。施用EDTA、SAP、CA和MA均对商陆根际土壤Cd和As含量产生显著影响，SAP处理对降低商陆根际土壤Cd含量的影响最显著，而MA处理对降低商陆根际土壤As含量的影响最显著。研究表明，EDTA、SAP、CA和MA均可提高商陆萃取农田土壤Cd和As的效率，尤其是EDTA和MA的效果更好。     

红毛丹果皮多酚氧化酶的酶促动力学研究

[目的]通过对红毛丹果皮多酚氧化酶的酶促动力学研究,寻找影响其催化活性的环境因素。[方法]以红毛丹果皮为试验材料,以邻苯二酚为底物,研究不同温度、不同pH、以及EDTA、Vc、亚硫酸氢钠、柠檬酸4种抑制剂和底物浓度对其多酚氧化酶活性的影响。[结果]红毛丹果皮多酚氧化酶的最适温度为40℃,最适pH值6.8;EDTA、Vc、NaHSO3、柠檬酸对多酚氧化酶活性表现出较好的抑制效果,其中EDTA的抑制效果较其他3种抑制剂强。[结论]利用低温、一定浓度的EDTA、Vc、NaHSO3、柠檬酸等试剂可抑制红毛丹果皮多酚氧化酶的催化活性,延长红毛丹果实的贮藏和运输时间,抑制其褐变;该研究结果为开发利用红毛丹果皮多酚氧化酶提供了理论依据。     

腐植酸、EDTA、Cr(Ⅵ)调控猪粪尿废水异化Fe(Ⅲ)还原偶联VFAs的转化能力比较

以人工合成Fe(OH)3作为电子受体,在猪粪尿废水中添加具有铁还原能力的菌株,厌氧恒温培养,通过对猪粪尿废水中添加不同浓度腐植酸、EDTA、Cr(Ⅵ),揭示3种典型理化因素调控猪粪尿废水中异化Fe(Ⅲ)还原偶联挥发性脂肪酸(Volatile fatty acids,VFAs)转化能力。结果表明,电子穿梭体腐植酸促进Fe(Ⅲ)还原,加速VFAs降解,而络合剂EDTA和重金属Cr(Ⅵ)则会阻碍Fe(Ⅲ)还原,减缓VFAs降解。虽然三种影响因子影响机理不同、途径不同,但最终Fe(Ⅲ)还原和VFAs平衡点不变。     

柠檬酸强化低浓度EDTA对成都平原农田土壤铅和镉的淋洗效率

为了寻找绿色高效的土壤重金属修复技术,研究了柠檬酸与低浓度EDTA不同组合方式对成都平原两类污染农田土壤中Pb和Cd的淋洗率,并测定了淋洗前后土壤中有机质和基础养分含量。结果表明,EDTA单独淋洗时对水稻土Pb、Cd的最高淋洗率为44.94%和88.32%,对紫色土Pb、Cd的最高淋洗率为48.48%和71.85%。柠檬酸单独淋洗时对水稻土Pb、Cd的最高淋洗率为24.71%和69.38%,对紫色土Pb、Cd的最高淋洗率为33.54%和59.77%。柠檬酸与低浓度EDTA复合淋洗效果均优于单独淋洗,其中先EDTA再柠檬酸淋洗组合效果最好。相较于EDTA单独淋洗,该组合方式将水稻土中Pb、Cd的淋洗率分别提高了6.07%和9.67%,将紫色土中Pb、Cd的淋洗率均提高了10.33%和14.73%。同时,复合淋洗还能减少10%以上的土壤有机质与基础养分的流失。综合淋洗效率和土壤有机质及养分的变化,先用EDTA淋洗再用柠檬酸淋洗是最佳淋洗组合。     

α-苯氧苯基-甘氨酸的合成及生物活性研究

[目的]以二苯醚为母体衍生出氨基酸基团,改善二苯醚类化合物的传导性。[方法]从苯氧基苯甲醛出发,合成了不同取代的α-苯氧苯基-甘氨酸,并以水稻、黄瓜种子为试材,利用小杯法测定了目标化合物在光照和黑暗条件下对单、双子叶植物种子室内根、芽生长的影响;以3～4叶期玉米为材料,利用再生苗法检测了目标化合物在玉米苗中的传导性。[结果]供试化合物对水稻、黄瓜的根、芽生长基本表现出抑制作用。此外,在黑暗条件下,化合物I对水稻的根、芽在低浓度下表现出促进生长的作用,高浓度下表现出抑制生长的作用;在光照条件下,化合物I、II对黄瓜的根在低浓度下表现出促进生长的作用,在高浓度下表现出抑制生长的作用。2 000 mg/L目标化合物药液处理玉米苗后,对再生苗生长有明显的抑制作用。[结论]二苯醚衍生氨基酸基团后,其生物活性和光活化活性得到了保留,具有潜在的除草剂或者生长调节剂的功能,并能改善二苯醚类化合物本身在植物韧皮部向下传导的能力。     

柱淋滤-巯基改性坡缕石钝化组合对弱碱性土壤镉铅污染控制效应

为研究弱碱性土壤镉(Cd)、铅(Pb)污染的控制技术,本文开展室内柱淋滤和巯基改性坡缕石钝化试验,探究柱淋滤-钝化组合对弱碱性土壤Cd和Pb污染控制效应。结果表明,乙二胺四乙酸(EDTA)和乙二胺二琥珀酸(EDDS)对弱碱性土壤Cd和Pb的淋滤效果优于谷氨酸二乙酸(GLDA),适宜淋滤浓度为3 mmol·L^-1。其中,EDTA、EDDS对土壤中Cd和Pb的总淋出率分别为58.60%和30.60%、44.78%和24.33%。随淋滤液体积的增加,淋出液pH值均逐渐增加,在淋出液体积为400 mL后逐渐趋于稳定。与原土相比,采用H2O、EDTA、GLDA和EDDS淋滤后,土壤中DTPA提取态Cd和Pb含量分别降低32.09%和16.08%、67.16%和33.64%、33.58%和18.93%、75.37%和51.15%。淋滤后土壤经0.3%巯基改性坡缕石(MPAL)钝化后DTPA提取态Cd和Pb浓度显著降低,与H2O+MPAL组合处理相比,EDTA+MPAL、GLDA+MPAL和EDDS+MPAL组合处理后土壤中DTPA提取Cd和Pb含量降低率分别为98.26%和48.01%、97.88%和45.80%、98.26%和55.59%。巯基改性坡缕石钝化可将土壤中可交换态和碳酸盐结合态Cd和Pb转变为稳定的铁锰氧化态Cd和Pb。研究表明:EDDS+MPAL组合效果较好,可显著降低土壤Cd和Pb总量和可交换态Cd和Pb含量,实现对弱碱性中重度Cd和Pb污染土壤重金属总量减少和有效态含量降低的减总降效双重控制作用。     

可溶性有机物和pH值对铅的毒性和生物可利用性的影响

采用芥菜发芽试验研究了柠檬酸(CA)、苹果酸(MA)、草酸(OA)、乙二胺四乙酸(EDTA)、二乙烯三胺五乙酸(DPTA)、腐植酸(HA)和土壤提取液(A)对Pb2 的毒性和生物可利用性的影响。结果表明,低分子量有机酸柠檬酸和苹果酸降低了Pb2 的毒性,但增加了Pb2 的生物可利用性,低浓度乙二胺四乙酸(EDTA)、草酸(OA)和二乙烯三胺五乙酸(DTPA)均降低了Pb2 的毒性和生物可利用性;相反,腐植酸增加了Pb2 的毒性,降低了生物可利用性。pH<5时,上述低分子量有机酸增加了Pb2 的毒性和生物可利用性;pH>5时,则降低了Pb2 的毒性和生物可利用性。土壤中Pb2 的生物可利用性和毒性效应取决于土壤溶液中共存有机物质的性质和溶液的pH值。     

Cr3+对土壤脲酶活性特征的影响

采用模拟方法对Cr~(3+)的土壤脲酶效应进行了研究,结果表明,土壤pH对Cr~(3+)的生态毒性有重要影响;酸性土壤脲酶受到显著抑制,活性及动力学特征参数与Cr~(3+)浓度间达显著或极显著负相关,而且模型U=β0/(β1xC+1)揭示其间机理为完全抑制,动力学则进一步细化为非竞争性抑制;获得土壤轻微和中度污染时的生态剂量ED10和ED50分别为50.59和865.7 mg·kg~(-1);酸性土壤中脲酶活性、Vmax、k可作为土壤Cr~(3+)亏染的监测指标之一,而碱性土壤则反应不敏感,其随铬浓度增加,脲酶活性及动力学参数呈先增加后降低的规律性变化,总体变幅较小;两类土壤的差别可能主要是由于土壤环境引起了不同价态铬转变的缘故.     

Allelopathy of decomposed maize straw products on three soilborn diseases of wheat and the analysis by GC-MS

In northern China, the soil-born diseases of wheat have been getting more and more serious under a new farming system that returns maize straw to the field. In order to investigate the allelopathy of the decomposed maize straw products on three soil-born diseases of wheat, culture dish and pot experiments were conducted and the compounds in the products were identified by gas chromatography-mass spectrometry(GC-MS). Culture dish experiments showed that the mycelial growth, sclerotia formation amount and total weight of Rhizoctonia cerealis were promoted at concentrations of 0.03, 0.06 and 0.12 g m L–1 and inhibited at concentration of 0.48 g m L–1 of the decomposed products. No significant effects were found of the product concentrations on average weight of the sclerotia. Mycelial growth of Gaeumannomyces graminis was promoted at almost all concentrations except the highest one. Mycelial growth and spore germination of Bipolaris sorokiniana were significantly inhibited by all concentrations of the decomposed products, with enhanced inhibition effects along with the increased concentrations. The length, number and dry weight of roots together with the root superoxide dismutase activity were promoted by the lowest concentration(0.03 g m L–1), with a synthetic effect index of 0.012, and inhibited by other concentrations. The ion leakage of roots was increased and the root peroxidase activity of roots was lowered by all the treatments. Pot experiments revealed that occurrence of the sharp eyespot was reduced by 0.03 and 0.06 g m L–1 of decomposed products after irrigation. However, the incidence rates and disease indexes were significantly increased by 0.12, 0.24 and 0.48 g m L–1 of decomposed products. The results indicated that incidence rates and disease indexes of the take-all were significantly promoted after being irrigated with the decomposed products, while occurrences of the common rot didn't change, significantly. GC-MS results showed that the compounds of the decomposed products included organic acids, esters, hydrocarbons, amides and aldehydes, with the proportions 25.26, 24.01, 17.22, 14.39 and 7.73%, respectively. Further analysis investigated that the allelochemicals identified in straw decomposed products contained p-hydroxybenzoic acid(9.21%), dibutyl phthalate(6.94%), 3-phenyl-2-acrylic(5.06%), 4-hydroxy-3,5-dimethoxybenzoic acid(2.26%), hexanoic acid(1.73%), 8-octadecenoic acid(1.06%), 3-(4-hydroxy-3-methoxy-phenyl)-2-propenoic acid(1.04%), 4-hydroxy-3-methoxy-benzoic acid(0.94%) and salicylic acid(0.94%).     

螯合剂调控下短毛蓼对锰污染土壤的修复潜力

采用盆栽试验,通过对锰尾矿区、恢复区和模拟锰污染土壤中加入不同质量摩尔浓度的抗坏血酸、酒石酸和EDTA,研究加入螯合剂对超富集植物短毛蓼累积土壤中锰的影响。结果表明：短毛蓼对不同土壤中锰的累积效果有很大不同,添加螯合剂能显著提高短毛蓼对尾矿区和恢复区锰的累积,却抑制了其对模拟区锰的吸收;EDTA对提高短毛蓼富集、转运土壤中锰的影响明显大于抗坏血酸和酒石酸,其中,10 mmol/kg EDTA对尾矿区短毛蓼地上部富集能力的影响达到了最大;植物对重金属的迁移总量与地上部生物量有密切关系,而螯合剂对植物有一定毒害作用,如果浓度过高会严重影响植物的产量,如10 mmol/kg EDTA使地上部的生物量减产过半。总的来说,不同浓度的不同螯合剂对短毛蓼累积锰的调控效应各不相同,从修复潜力和潜在的环境风险来看,5 mmol/kg EDTA是提高锰尾矿区短毛蓼对锰的转运能力和修复效率的最佳调控螯合剂。