串叶松香草不同生育期营养物质及瘤胃降解动态

对不同生育期串叶松香草的营养物质变化以及干燥方法（烘干、自然晒干）对其营养成分的影响进行了研究,并进一步探讨其瘤胃降解动态趋势。结果表明,随着生育期的推迟,串叶松香草营养物质变化同其他牧草相一致,但是其粗蛋白质含量下降速度较快,从莲座叶丛期到抽茎期其降幅可达25.62%。试验还表明,其再生草品质略低于头茬牧草,烘干可保存较多的营养物质获得优质的干草。根据牧草不同生育期营养成分的变化以及牧草各营养成分的瘤胃降解率,建议串叶松香草在抽茎—现蕾期刈割利用。     

Effect of Adding Different Mannan Oligosaccharide to Diets with Different Concentrate to Forage Ratios on Rumen Degradability of Fiber in Sheep

To investigate the effect of adding mannan oligosaccharide to diets with different concentrate to forage ratios on the degradability of neutral detergent fiber (NDF) and acid detergent fiber(ADF) in rumen of sheep by nylon bags technique,6 wethers (White Suffolk♂×Small-tailed Han sheep♀) installed permanent rumen fistula were used for offering rumen fluid.The 4×6 two-factor experimental design was chosen and the two factors were the ratio of concentrate to forage (A1(20:80),A2(30:70),A3(40:60),A4(50:50)) and the dose of mannan oligosaccharide (B1 (0),B2 (0.4%),B3 (0.8%),B4 (1.2%),B5 (1.6%),B6 (2.0%)).The results showed that the rumen degradability of NDF and ADF in different times,degradation parameters and effective degradability were influenced by the factor A significantly (P< 0.05).The rumen degradability of NDF and ADF in each time of group A1 were higher than group A3 and A4 (P< 0.05).Expect for 24 h,the rumen degradability of ADF were influenced significantly by the factor B,and the rumen degradability of ADF in group B5 was higher than group B1 and B2 (P< 0.05).The rumen effective degradability of NDF in group A3 was significantly higher than groups A1 and A2 (P< 0.05), and a little higher than group A4 (P >0.05),which showed that the rumen effective degradability of NDF and ADF were higher when the ratio of concentrate to forage was 40:60.     

不同精粗比日粮添加甘露寡糖对绵羊瘤胃纤维降解率的影响

选取6只安装永久瘤胃瘘管羯羊(白萨福克♂×小尾寒羊♀)为瘤胃液供试动物,采用4×6二因子析因试验设计,共设2个因子,分别为不同精粗比日粮(A1(20:80)、A2(30:70)、A3(40:60)、A4(50:50))和不同甘露寡糖添加量(B1 (0)、B2 (0.4%)、B3 (0.8%)、B4 (1.2%)、B5 (1.6%)、B6 (2.0%)),进行尼龙袋半体内试验,旨在研究不同精粗比日粮中添加甘露寡糖对绵羊瘤胃中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)降解率的影响。结果表明,A因子对日粮中不同时间点NDF、ADF瘤胃降解率、降解参数及有效降解率均产生了显著影响(P< 0.05),A1处理组的NDF和ADF各个时间点(除3 h外)的瘤胃降解率均显著高于A3和A4处理组(P< 0.05);B因子对3、6、9、12、48 h的瘤胃ADF降解率均产生了显著影响(P< 0.05),其中B5处理组显著高于B1、B2处理组(P< 0.05);A3处理组的NDF有效降解率显著高于A1、A2处理组(P< 0.05),稍高于A4处理组(P >0.05)。综上所述,在精粗比为40:60时,NDF和ADF的瘤胃有效降解率处于较高水平。     

裹包全混合日粮瘤胃降解特性的研究

试验选用2头安装有永久性瘤胃瘘管的荷斯坦奶牛作为试验动物,采用尼龙袋法研究了裹包贮存、精粗比和水分含量对全混合日粮(TMR)营养成分瘤胃降解特性的影响。结果表明:裹包贮存可显著提高TMR饲料干物质(DM)、粗蛋白(CP)和中性洗涤纤维(NDF)的瘤胃消失率和有效降解率(P0.05)。DM和CP的瘤胃消失率及有效降解率随TMR饲料精料水平的提高显著增加(P0.05)。水分含量60%组TMR饲料中CP的有效降解率显著高于40%和50%组(P0.05),40%和50%组间差异不显著(P0.05)。因此,裹包贮存可提高TMR饲料中营养物质瘤胃消失率和有效降解率(P0.05),营养物质的瘤胃消失率和有效降解率随TMR饲料精粗比的增加而增加,水分含量对裹包TMR中不同营养物质的消失率和有效降解率有不同影响。     

Influences of grassland degradation on forage availability by sheep in the Inner Mongolian steppes of China

In China and throughout the world, evaluation of the nutrient values of forages has always lagged behind research regarding the nutrient requirements of animals. In this study, the effective degradability and availability of forages were evaluated on undegraded and degraded grassland using a technique where nylon bags were incubated in sheep rumen. We found that the concentrations of organic matter (OM), neutral detergent fiber (NDF), non‐fiber carbohydrates (NFC) and carbohydrates (CHO) of forage were higher (P < 0.05) and the crude protein (CP) concentration was lower (P < 0.05) in the degraded grassland than in the undegraded grassland. The effective degradability (ED) of NFC (ED_NFC) was higher (P < 0.05) and ED_CHO and ED_NDF were lower in the undegraded grassland than in the degraded grassland. The ED_OM and ED_CP were not affected by the type of grassland, which may explain why ED is an inadequate parameter for the evaluation of forage availability. According to an analysis of the ratio of effectively degraded N to OM and NFC, plants of the undegraded grassland can provide more digestive nutrients to meet the requirements of grazing animals compared to plants of the degraded grassland. Results suggested that it is inadequate to consider only chemical composition and effective degradability when evaluating forage availability with grassland degradation. The ratio of effectively degraded N to OM and NFC was an appropriate indicator.     

山西不同居群白羊草的营养成分及瘤胃降解规律

为研究山西不同居群白羊草抽穗期的营养成分含量及干物质(DM)和粗蛋白质(CP)在绵羊瘤胃中的降解规律,以安装永久性瘘管的杜泊与本地羊杂交一代肉用绵公羊15只为试验动物,采用尼龙袋法对不同居群白羊草抽穗期的DM和CP的降解率及降解参数进行测定。结果表明,居群对白羊草的常规营养成分含量有较大的影响。其中,太谷居群具有最高的粗蛋白质(CP)含量和最低的中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)含量,其次是原平和沁源居群。不同居群白羊草的DM和CP的降解特性不同,其中,太谷和原平居群72 h的DM降解率最高,显著高于襄汾居群(P<0.05),太谷居群的DM有效降解率最高,达到46.39%,其次是原平和沁源居群;太谷和原平居群72 h同样有最高的CP降解率,显著高于降解率最低的芮城居群(P<0.05)。太谷居群的CP有效降解率最高,其次是方山和阳高居群,三者差异不显著(P>0.05)。由此可见,太谷居群DM和CP降解率最高,优于其他居群。     

不同日粮组成下高寒地区小黑麦在断奶初期奶公犊瘤胃内动态降解的研究

用3头3月龄左右、装有瘤胃瘘管的荷斯坦奶公犊,按3×3完全拉丁方试验设计,喂给三种日粮,日粮组成按精粗比分别为:Ⅰ组,70:30;Ⅱ组,60:40;Ⅲ组,50:50,研究不同日粮组成下高寒地区小黑麦的DM、CP以及NDF在断奶初期奶公犊瘤胃内动态降解。试验结果表明:日粮精粗比为60:40时,其CP的降解率和降解参数明显低于精粗比为70:30和50:50的日粮,差异显著(P<0.05);其DM和NDF的降解率和降解参数明显高于精粗比为70:30和50:50的日粮,差异显著(P<0.05)。     

脲酶抑制剂氢醌对纤维类物质瘤胃降解率的影响

选用9只瘘管羊，采用完全随机区组设计（以体质量为区组）研究日粮中分别添加30mg/kg和60mg/kg氢醌对干物质、中性洗涤纤维（NDF）和酸性洗涤纤维（ADF）在瘤胃中降解率和降解参数的影响。结果表明，在日粮中添加30mg/kg和60mg/kg氢醌可以明显提高干物质、NDF和ADF的有效降解率（P＜0.05)，但对外流速度（k)没有显著影响（P＞0.05)。应用脲酶抑制剂可以显著提高NDF的48h降解率，ADF的24h和48h的降解率（P＜0.05)。上述结果表明，在含有尿素的日粮中添加适宜水平的氢醌有利于氨的释放与底物发酵同步，从而导致了纤维物质消化率的提高。     

刈割时间与加工方法对柠条营养价值的影响

采集开花期、结实期和往年生结实期柠条,分别揉碎、切碎和粉碎。用3只装有永久性瘤胃瘘管绵羊测定柠条消化率和瘤胃降解率。用81只大尾寒羊,按随机区组设计分为9组,进行饲养试验。结果表明:结实期柠条粗蛋白含量高达16.16%,较开花期高14.29%,较往年生老枝高93.76(%P<0.01);中性洗涤纤维含量46.08%,较开花期低38.30%,较往年生老枝低55.88%;结实期揉碎柠条养分有效降解率、消化率、采食率、日增重等指标明显优于其他处理(P<0.01),说明以结实期刈割当年生柠条、揉碎加工饲用价值最好。     

氨化对油菜秸秆营养成分及山羊瘤胃降解特性的影响

本试验旨在研究氨化对油菜秸秆营养成分及山羊瘤胃降解特性的影响。将粉碎的油菜秸秆用30%水和不同比例(10%、15%、20%)碳酸氢铵进行氨化处理,并于处理后7、14和21 d采集样品,与未氨化处理的油菜秸秆(对照)同时进行营养成分分析。然后采用尼龙袋法测定氨化和未氨化处理油菜秸秆的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的瘤胃降解率。结果表明:氨化后油菜秸秆CP含量增加,粗脂肪(EE)、NDF和ADF含量下降,DM、粗灰分含量基本保持不变。氨化组油菜秸秆DM和CP有效降解率均显著高于对照组(P0.05);15%、20%碳酸氢铵氨化处理油菜秸秆ADF有效降解率均显著高于对照组(P0.05)。综合得出,添加15%和20%碳酸氢铵氨化能显著提高油菜秸秆DM、CP和ADF的山羊瘤胃降解率,油菜秸秆经15%碳酸氢铵、30%水分条件下氨化处理效果最好、最经济。     

Rumen Degradation Characteristics of Six Types of Common Roughage in Khorchin Beef Cattle in Tongliao City

[Objective] The paper was to study the degradation characteristics of different types of roughage in Khorchin beef cattle. [Method] The nylon bag method was used to determine the rumen degradability of six types of roughage: dry matter(DM), crude protein(CP), neutral detergent fiber(NDF) and acid detergent fiber(ADF). [Result] Among the six types of roughage, alfalfa hay had the highest effective degradability of DM and CP in rumen; followed by alfalfa block, corn silage, leymus chinensis and corn stover, and the effective degradability of rice straw was lowest. In terms of effective degradability of NDF and ADF, alfalfa hay was the most effective, while leymus chinensis was the least effective. [Conclusion]Considering degradation characteristics of six types of roughage in Khorchin beef cattle, alfalfa hay possesses the highest nutritional value, while rice straw has the lowest value.     

Digestibility, methane production and nitrogen balance in sheep fed ensiled or fresh mixtures of sorghum–soybean forage

The feeding value of a mixture of sorghum and soybeans plants, either fresh or ensiled, was evaluated with sheep. Sorghum and soybeans were harvested during the Cuban rainy season and ensiled in a ratio of 0.6/0.4 (w/w, as feed) with molasses and a bacterial inoculant. Silos were opened between 162 and 182 d post ensiling during the Cuban dry season and silages were fed to six pelibuey sheep (including two fistulated). Six other sheep (also including two fistulated) were fed sorghum and soybean in the same proportion, but freshly harvested during the dry season. The experiment lasted 21 d (14 d adaptation and 7 d data collection period). Silage quality parameters included pH, ammonia, lactate, and short chain fatty acids (SCFA). Further, both fresh and ensiled diets were offered to study the rumen fermentation characteristics (pH, ammonia, lactate, SCFA, in situ degradability and methane), duodenal flow of microbial protein (assessed through urinary purine derivatives secretion), fecal degradability of nutrients and urinary N excretion. From these measurements ME value and degradable CP supply at the small intestine (DCPSI) were estimated. Silage was of excellent quality. Compared to fresh forage feeding silage increased molar propionate proportion and rumen microbial protein synthesis and reduced methane emission. Fresh forage showed lower rumen degradability and total digestibility. Further, the higher ME concentration (11.2 vs. 10.3 MJ/kg DM) and DCPSI (84.7 vs. 56.1 g/kg DM) of the silage would allow a higher milk production or daily gain as compared with fresh forage available during the Cuban dry season.     

青贮对饲料稻秸秆DM和NDF瘤胃降解特性的影响

选用3头带永久性瘤胃瘘管的中国荷斯坦泌乳奶牛,以直接晒干饲料稻Oryza sativa秸秆(HRS)为对照,鲜湿稻秆青贮(SRS)为试验组,研究了青贮对饲料稻湘早籼24号秸秆干物质(DM)和中性洗涤纤维(NDF)瘤胃降解特性的影响.结果表明:青贮显著降低了NDF的含量(P<0.05),对粗蛋白(CP)、粗脂肪(EE)、无氮浸出物(NFE)和酸性洗涤纤维(ADF)无显著影响(P>0.05);青贮极显著提高了饲料稻6、12、24 h时间点的DM和NDF的瘤胃消失率(P<0.01),显著提高了48和72 h时间点的DM和NDF的瘤胃消失率(P<0.05),对36 h时间点DM和NDF瘤胃消失率无显著影响(P>0.05);青贮极显著提高了DM和NDF慢速降解部分(b)和其降解速度常数(c)(P<0.01),对快速降解部分(a)无显著影响(P>0.05);极显著提高了DM和NDF瘤胃有效降解率(P<0.01).结论:青贮可改善饲料稻秸秆养分DM和NDF的瘤胃降解特性,是合理利用水稻秸秆,挖掘其饲用价值的有效途径.     

不同发酵时间对全混合日粮的瘤胃降解特性以及小肠消化率的影响

试验旨在研究发酵0、3、7、15、30 d的全混合日粮(TMR)对瘤胃降解特性以及小肠消化率的影响。选用3头体重为550 kg左右、安装有永久性瘤胃瘘管的荷斯坦奶牛,采用尼龙袋法和三步体外法测定发酵全混合日粮(FTMR)的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)的瘤胃降解率以及瘤胃未降解蛋白(RUP)的小肠消化率。结果表明:随着发酵时间的延长,FTMR饲料的DM含量显著降低(P<0.05);随着发酵时间的延长,显著提高FTMR的DM、CP、NDF的瘤胃降解率(P<0.05)和CP、NDF的快速降解部分(P <0.05);同时发酵显著降低RUP的小肠消化率(P <0.05)。综上可知,全混合日粮提高了DM、CP和NDF在瘤胃中的瘤胃降解率,15、30 d的FTMR各营养成分的瘤胃降解性较好;RUP的小肠消化率随发酵时间的延长开始逐渐降低,7d之后降低最为明显。因此,应控制好TMR的发酵时间,以便更有效地利用饲料资源。     

不同紫花苜蓿品种瘤胃降解特性比较

为研究不同紫花苜蓿（Medicago sativa L.）品种在牛瘤胃中的降解规律,本试验以10个紫花苜蓿品种为材料,以3头装有永久性瘘管的晋南牛作为试验动物,采用尼龙袋法测定不同紫花苜蓿品种干物质（DM）、粗蛋白质（CP）、中性洗涤纤维（NDF）和酸性洗涤纤维（ADF）的瘤胃降解率和降解参数。结果表明：不同紫花苜蓿品种DM,CP,NDF和ADF的降解率随着其在瘤胃内培养时间的延长,均呈升高趋势,但不同品种在同一培养时间段内的降解率存在显著差异;不同紫花苜蓿品种的有效降解率（ED）的差异较大,‘WL-319HQ DM’的ED最高,显著高于‘中苜一号’和‘首领’（P<0.01）;‘金皇后’CP的ED显著高于除‘赛迪’之外的其他品种;‘WL-168HQ’、‘中苜一号’NDF的ED显著高于除‘首领’之外的其他品种;‘三得利’、‘皇后’ADF的ED显著高于其他品种（P<0.05）。由此可见,不同紫花苜蓿品种在瘤胃内的降解特性不同。     

Characterization of season and sampling method effects on measurement of forage quality in fescue-based pastures

Information on seasonal changes and effects of sampling methods on the measurement of forage quality is limited for fescue-based pastures. Eight continuously grazed, 0.76-ha, fescue-based pastures were used to compare forage type, method of collection, and seasonal effects on forage quality in a repeated-measures, split-plot design. Four pastures were interseeded with red clover in March 2000. Masticate (M; from four ruminally cannulated steers) and hand--clipped (C) samples were collected every 28 d from April to October 2000. Interseeding red clover did not affect (P > 0.10) OM, CP, NDF, and ADF concentrations or CP degradability. Sampling method and season interacted (P < 0.03) for OM, CP, NDF, and ADF concentrations. Concentrations of OM averaged 5 percentage units more (P < 0.01) in C than in M in all months and were more variable with M than with C. Samples clipped between April and September averaged 5.5 percentage units greater NDF (P < 0.01), 3.0 percentage units greater ADF (P < 0.01), and 4.5 percentage units less CP (P < 0.01) than masticate samples obtained during the same time period. Fiber and CP concentrations did not differ (P > 0.10) between C and M samples obtained in October. Differences in CP degradability estimates (using Streptomyces griseus protease) between the two sample types were greater in late-season samples than in samples obtained from April to June. When S. griseus protein degradability estimates were compared with in situ estimates for masticate samples, no differences (P > 0.10) were detected early in the season (April to June). However, the S. griseus procedure overestimated in situ values (P < 0.01) by an average of 3 percentage units in samples obtained between July and October. Differences in composition of C and M samples were substantial until late season, when opportunities for selective grazing were minimal. Small differences between S. griseus and in situ estimates of CP degradability indicate that the S. griseus procedure can yield useful CP degradability estimates for fescue-based pasture samples. Although it might be possible to apply correction values to clipped samples to estimate CP and fiber concentrations of diets selected by grazing cattle, inconsistent relationships preclude this approach for estimates of CP degradability.     

西藏山南地区6种野生牧草营养价值评定

选用6只装有瘘管的西藏澎波半细毛羊,用尼龙袋法对采于西藏山南地区的6种野生禾本科牧草的营养成分含量及其在绵羊瘤胃内的降解特性进行了测定,分析评价其营养利用价值。结果表明:6种牧草的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的瘤胃降解消失率和降解特征存在很大差异;随着样品在瘤胃内停留时间的延长,降解消失率也随之提高,在3-48 h时,增加的幅度较快,48h后,增加的速度放缓;到72 h,狗尾草(Setaria viridis (L.) Beauv.)的降解消失率最大,其DM、CP和NDF的降解消失率均超过60%;蛋白质较高、纤维含量较低的狗尾草和白草(Pennisetum centrasiaticum Tzvel.),其DM、CP、NDF和ADF的快速降解部分、慢速降解部分及有效降解率均比其他4种牧草高;而白茅(Imperata cylindrical (L.) Beauv.)和沙生针茅(Stipaplareosa P.Smirn.)营养成分表现出比其他4种牧草差的降解特征。综合考虑6种牧草所含营养成分及其在绵羊体内的降解能力,狗尾草和白草利用价值较高,白茅和沙生针茅利用价值较低。     

2种决明属牧草不同生育期营养成分在肉牛瘤胃降解动态研究

采用瘤胃尼龙袋法,研究威恩圆叶决明(Chamaecrista rotundifolia cv.Wynn)和闽引羽叶决明(C.nictitans cv.Minyin)2个豆科牧草初花期和结荚期干物质(DM)、中性洗涤纤维(NDF)、粗蛋白质(CP)等养分在肉牛瘤胃内0～72 h降解动态变化,并建立瘤胃降解模型参数。结果表明:不同生育期决明属牧草各营养成分的瘤胃降解特性有着不同差异,牧草初花期的DM,NDF和CP在肉牛瘤胃72 h的有效降解率均高于结荚期。2个牧草品种的DM和NDF的慢速降解部分b值均随着生育期的延长而逐渐降低,但牧草DM的有效降解率差异并不显著。闽引羽叶决明初花期NDF的有效降解率(20.31%)显著高于结荚期(13.01%)(P<0.05);威恩圆叶决明初花期NDF的有效降解率(11.84%)比结荚期(9.12%)高出29.82%,但差异不显著。闽引羽叶决明初花期的CP快速降解部分a值和慢速降解部分b值均显著大于结荚期(P<0.05),有效降解率分别达到65.99%和58.76%;威恩圆叶决明初花期和结荚期CP的快速降解部分a值、慢速降解部分b值及有效降解率均无显著差异。不同品种、不同生育期DM和CP的有效降解率均要高于NDF的有效降解率。此外,从品种间营养成分的瘤胃降解特性综合比较,闽引羽叶决明在瘤胃的降解效果要优于威恩圆叶决明。     

常规与非常规粗饲料在奶牛瘤胃中的降解特性研究

旨在探讨非常规粗饲料(玉米芯、毛豆荚壳)与奶牛常用粗饲料(苜蓿干草、玉米青贮)在奶牛瘤胃中的降解规律,试验选用3只装有永久性瘤胃瘘管的荷斯坦奶牛,采用尼龙袋法测定4种粗饲料的干物质(DM)、粗蛋白(CP)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的瘤胃降解规律。结果表明:1)苜蓿干草和毛豆荚壳的CP含量分别为17.55%和11.10%,且苜蓿干草显著高于毛豆荚壳(P<0.05);玉米芯和玉米青贮的CP含量分别为2.97%和8.41%,且玉米芯显著低于玉米青贮(P<0.05);玉米芯的NDF和ADF含量显著高于其他3种粗饲料(P<0.05),达到79.98%和43.35%。2)苜蓿干草的DM有效降解率最高(P<0.05),由高到低依次为苜蓿干草、毛豆荚壳、玉米青贮和玉米芯。毛豆荚壳的CP有效降解率显著高于其他3种粗饲料(P<0.05),由高到低依次为毛豆荚壳、苜蓿干草、玉米青贮和玉米芯。苜蓿干草的NDF有效降解率显著高于毛豆荚壳(P<0.05),玉米青贮和毛豆荚壳无显著差异(P>0.05);玉米芯的ADF有效降解率最高(P<0.05),苜蓿干草、玉米青贮和毛豆荚壳无显著差异(P>0.05)。综上所述,玉米芯和毛豆荚壳可作为非常规粗饲料资源加以开发利用。     

Effect of supplementation of dried distillers grains with solubles on forage intake and characteristics of digestion of beef heifers grazing small-grain pasture

Sixteen ruminally cannulated, English-crossbred heifers (378 ± 28.4 kg) grazing small-grain pasture (SGP) were used in a completely randomized design to evaluate effects of supplementing different amounts of corn dried distillers grains with solubles (DDGS; 0, 0.2, 0.4, and 0.6% of BW; as-fed basis) on forage intake, digestibility, and rumen fermentation characteristics. The experiment was conducted from April 6 through April 20, 2007. Heifers grazed in a single SGP with supplements offered individually, once daily at 0700 h. Forage and total OM, CP, and NDF intake were not affected (P ≥ 0.21) by DDGS amount. Digestibility of NDF and ether extract (EE) increased linearly (P < 0.001) when heifers consumed more DDGS. Intake of DM (kg/d and g/kg of BW), ruminal volume (L), fluid dilution rate (%/h), fluid flow rate (L/h) turnover time (h), and particle dilution rate (SGP and DDGS) were not affected (P ≥ 0.32) by increasing DDGS supplementation amount. In situ DDGS CP kinetic parameters were not affected (P ≥ 0.25) by increasing DDGS supplementation amount. Forage masticate in situ soluble CP fraction and CP effective degradability increased quadratically (P = 0.01) with increasing DDGS supplementation amount. However, amount of DDGS did not affect forage masticate CP slowly degradable fraction (%; P = 0.39) or degradation rate (%/h; P = 0.63). Rate of in situ disappearance (%/h) for DDGS DM (P = 0.94), forage masticate DM (P = 0.89), and NDF (P = 0.89) were not affected by DDGS supplementation amount, nor was rumen undegradable intake protein (% of CP) for DDGS (P = 0.28) and forage masticate samples (P = 0.93). Ruminal concentration of VFA and ammonia and ruminal pH were not affected (P ≥ 0.21) by increasing DDGS amount. Results indicated that DDGS can be used in SGP supplements without negatively affecting forage intake, digestibility, or ruminal fermentation.