Hypoglycemic Effects of the n-Butanol Part of Sorbus tianschanica

[Objective] To extract and isolate the effective components from Sorbus tianschanica,and to research the hypoglycemic effects of n-Butanol part. [Methods]S. tianchanica was extracted by reflux extraction. After gradient elution,n-Butanol part was obtained. With diabetic mice induced by STZ as the model,blood glucose test was carried out. [Results]n-Butanol part in administration group had significant differences in hypoglycemic effects with that in model group(P 0. 05),showing that n-Butanol part had certain antidiabetic functions on diabetic mice induced by STZ. And the hypoglycemic effects showed a reducing trend as the administration dosage decreased,indicating that administration dosage had positive correlation with hypoglycemic effects. n-Butanol in different parts could reduce the blood glucose of diabetic mice in different degrees. And the blood glucose value in high-dosage group(8 g /kg) reduced greatly at 2 h after administration,having great differences with that in model group(P 0. 01). This showed that high dosage was helpful to the glucose tolerance of diseased mice,and could effectively protect against the rapid increase of blood glucose after meal. n-Butanol had no significant effects on the blood glucose of normal mice. However,the morality rates in high-and middle-dosage groups were both higher than that in low-dosage group; and the morality rate in administration group was lower than that in model group,showing that n-Butanol could promote the survival rate of diabetic mice. [Conclusions]S. tianchanica had certain effects of anti-diabetes.     

Pharmacodynamic Effects of Aquilaria sinensis Leaves and Its Comparison with LIGNUM AQUILARIAE RESINATUM in Pharmacological Actions

[Objective]To research the pharmacodynamic effects of Aquilaria sinensis leaves,and to compare pharmacological actions between A. sinensis leaves and LIGNUM AQUILARIAE RESINATUM. [Methods]Pharmacological experiments of analgesia,anti-inflammation,hypoglycemic action,relieving asthma and promoting intestine moving activity were carried out by whole animal tests. [Results] glucose values of diabetic mice induced by alloxan reduced significantly in LIGNUM AQUILARIAE RESINATUM group and high-dosage group of A. sinensis leaves( P 0. 05 or P 0. 01). Different dosage groups of A. sinensis leaves had extremely significant inhibitory effects on the auricle swelling of mice induced by xylene and the abdominal capillary permeability of mice induced by acetic acid( P 0. 01 or P 0. 05). Low- and middle-dosage groups of A. sinensis leaves and LIGNUM AQUILARIAE RESINATUM group had significant inhibitory effects on pains induced by thermal stimulus and acetic acid( P 0. 05 or P 0. 01),and obviously prolonged the asthma latency time of guinea pigs( P 0. 05);and high-dosage group of A. sinensis leaves also obviously prolonged the asthma latency time of guinea pigs( P 0. 05). Water extracts of A. sinensis leaves showed significant differences in time to falling asleep and sleeping time of mice( P 0. 01 or P 0. 05). Different dosage groups of A. sinensis leaves and LIGNUM AQUILARIAE RESINATUM group significantly enhanced the intestine moving distance and rate( P 0. 05,P 0. 01 or 0. 001). [Conclusions]Ethanol extracts from A. sinensis leaves and LIGNUM AQUILARIAE RESINATUM had certain functions ofanalgesia,anti-inflammation,hypoglycemic action,relieving asthma and promoting intestine moving activity. Effects of ethanol extracts from A. sinensis leaves were equal to the effects of LIGNUM AQUILARIAE RESINATUM; and the ethanol extracts from A. sinensis leaves was superior to LIGNUM AQUILARIAE RESINATUM in anti-asthma and promoting intestine moving activity.     

The Acute Toxicity and Subchronic Toxicity Test of Zhuqin Extract

[Objective] To investigate the safety of compound Zhuqin extract.[Methods]In acute toxicity test,mice in test group were given compound Zhuqin extract( i. g.) by gavage,the dosage of which was 0. 8,0. 6,0. 45,0. 35 g /20 g,to determine the median lethal dose of compound Zhuqin extract( LD50). In maximum tolerance experiment,mice in test group were given compound Zhuqin extract( i. g.) by gavage,the dosage of which was 0. 5 g /20 g,to determine the maximum tolerance dose( MTD); In the sub- chronic toxicity test,rats in test group were given compound Zhuqin extract( i. g.) by gavage for 30 d,the dosage of which was 2. 0,1. 5,1. 0 g /200 g,and normal saline negative control group was set. Clinic condition of rat was observed everyday,hematology,blood biochemistry,anatomy,organ index and organization pathological observation were conducted after test.[Results]The acute toxicity test failed to detect median lethal dose of compound Zhuqin extract; the weight differences were not significant( P 0. 05); some blood biochemical criterion and blood routine index had significant differences( 0. 01 P 0. 05),and some indexes had extremely differences( P 0. 01); organ index,necropsy and histopathology had abnormalities.[Conclusions] Compound Zhuqin extract was safe and reliable.     

Protective Effects of Coriolus versciclor Polysaccharides on Liver Injury of Mice Induced by CCl_4

[Objective] To discuss the protective effects of Coriolus versciclor polysaccharides on liver injury of mice induced by CCl4.[Methods]40 male Kunming mice at four moths old were randomly divided into four groups,which were control group,model group,C. versciclor polysaccharides protection group and feeds + C. versciclor polysaccharides group. Mice were fed for 8 d. On 2 h after final administration,intraperitoneal injection of blend oil solution was carried out was carried out in control group. And in other groups,intraperitoneal injection of0. 15% CCl4 blend oil solution( 10 mL / kg) was conducted. After 18 h,eyeballs were collected and serum was isolated. Livers were taken out and the hepatosomatic index was calculated. Liver homogenate was prepared and the ALT and AST were detected,as well as the SOD and NOS activities,and MDA,GSH and NO contents.[Results] Compared with model group,C. versciclor polysaccharides in different administration ways reduced the hepatosomatic index( P 0. 05) and MDA content( P 0. 001),lowered the AST activity in serum( P 0. 05),the NOS activity and the NO content( P 0. 01),and enhanced the SOD activity and GSH content( P 0. 01).[Conclusions] C. versciclor polysaccharides in different administration ways protected the liver injury of mice induced by CCl4.     

Application of Joint Detection of MYO,cTnI,CK-MB,D-D and hs-CRP in Acute Myocardial Infarction

[Objective] To discuss the diagnostic value of joint detection of myoglobin( MYO),cardiac troponin I( c Tn I),creatine kinase isoenzyme MB type( CK-MB),d-dimer( D-D),hypersensitive c-reactive protein( hs-CRP) to acute myocardial infarction( AMI).[Methods]A total of 293 cases of AMI patients were selected in the People's Hospital of Guangxi Zhuang Autonomous Region from June 2010 to December 2014,which were classified into AMI group. At the same time,136 suspected AMI patients were taken as the control group,who were finally excluded cases. MYO,CTn I,CK-MB,D-D and hs-CRP levels were detected in two groups. The detection results and diagnostic sensitivity and specificity were statistically analyzed. [Results]( 1) The five indices in AMI group enhanced significantly compared with those in control group( P 0. 01); MYO increased obviously after 2- 6; the five indices all enhanced; MYO reduced significantly after 12- 24 h;c Tn I,D-D and hs-CRP were still at relatively high levels after 24- 48 h.( 2) MYO,CK-MB,D-D and hs-CRP levels in left main coronary artery lesion group were significantly higher than three-vessel lesion group,double-vessel lesion group,and single-vessel lesion group( P 0. 05). Among them,three-vessel lesion group and double-vessel lesion group were significantly higher than single-vessel lesion group( P 0. 05). CK-MB levels in three-vessel lesion group and two-vessel lesion group showed no statistical significance( P 0. 05). c Tn I levels in different groups also had no statistical significance( P 0. 05).( 3) The sensitivity and specificity of joint detection were as high as 99. 4%and 98. 3%,respectively,which were significantly higher than those of single test. [Conclusions] Joint detection of MYO,c Tn I,CK-MB,D-D and hs-CRP was helpful to enhance the diagnostic rate of AMI,and could make up for the shortage of the single test,which was a practical test method worthy of clinical application.     

The Anti-inflammatory Effects of CORTEX PHELLODENDRI in Acute Inflammation Animal Model

[Objective] To research on the anti-inflammatory effects of CORTEX PHELLODENDRI in acute inflammation animal model.[Methods]Two acute inflammation animal models were established,including the auricle swelling test model of mice induced by xylene,and the abdominal capillary permeability model of mice induced by 1% glacial acetic acid. The anti-inflammatory effects of CORTEX PHELLODENDRI were observed. [Results]CORTEX PHELLODENDRI had different inhibitory effects on the auricle swelling of mice induced by xylene,and the abdominal capillary permeability of mice induced by 1% glacial acetic acid. The alkaloid part had significant differences with model group(P 0. 05 or P 0. 01). [Conclusions]Alkaloid part had good anti-inflammatory effects; while the effects of n-butanol,ethyl acetate,chloroform and petroleum ether parts were in different degrees.     

The Protection and Regulatory Effects of Yupingfeng Polysaccharides on Mice with Immune Function Damage

[Objective] To discuss the protection and regulatory effects of Yupingfeng polysaccharides on mice with immune function damage.[Methods]The immunosuppressive mice mode was established by 80 mg/kg cyclophosphamide. Effects of different dosages of Yupingfeng polysaccharides( 150,300,600 mg / kg) on structure and function recovery of mice immune organs were observed. [Results]The thymus and spleen in mice enlarged significantly; spleen index of mice increased obviously; splenic lymphocyte proliferation and transformation capacitates and NK cell activity all increased significantly( P 0. 05 or P 0. 01). Besides,spleen structure was damaged with compensatory hyperplasia of lymphoid tissue. Observation of polysaccharides positive control group( no administration of cyclophosphamide) showed that after mice were stimulated by Yupingfeng polysaccharides,spleens showed structural hyperplasia and hypertrophy. [Conclusions]Yupingfeng polysaccharides significantly promoted the structural and functional compensation of spleen after immunosuppression,and had obvious enhancement effects on spleen functions of normal mice.     

党参茎叶总皂苷提取工艺及其抗氧化活性研究

旨在优选党参茎叶中党参总皂苷的提取工艺条件,并分析其抗氧化性活性,为党参茎叶皂苷类资源的开发提供参考。以党参茎叶为材料,总皂苷的提取率为指标,研究乙醇浓度、液料比、提取温度和提取时间4个工艺参数对总皂苷得率的影响,在单因素试验的基础上,通过正交试验设计确定了乙醇回流提取的最佳工艺。在乙醇浓度70%,液料比25 mL/g,提取温度为70℃,提取时间为90 min的条件下党参茎叶总皂苷提取率达4.12%。体外抗氧化活性分析发现,党参茎叶总皂苷浓度与抗氧化活性呈正比,总皂苷对DPPH自由基、羟基自由基和超氧阴离子自由基的最大清除率分别为86.92%、55.37和82.21%,其IC50分别为0.37、0.78和0.44 mg/mL。通过简单的乙醇回流提取便可得到具有很好抗氧化效果的茎叶总皂苷,如将其继续开发为保健产品或食品添加剂,不仅可减少资源的浪费还可延伸党参产品的产业链,具有重要的生态意义和实践意义。     

Experimental Study on the Antitussive,Apophlegmatic and Antiasthmatic Effect of Extract of Jianhua

[Objective]To explore the antitussive,apophlegmatic and antiasthmatic effect of extract of Jianhua. [Methods]The antitussive, apophlegmatic and antiasthmatic effect of extract of Jianhua was studied by animal models. Control group,low-dosage group of extract of Jianhua,medium-dosage group of extract of Jianhua,high-dosage group of extract of Jianhua and positive medication group were included in the experiment. [Results] Compared with control group,low,medium and high-dosage of aqueous extract and alcohol extract of Jianhua could significantly inhibit cough induced by stronger ammonia water( P 0. 05,P 0. 01); medium and high-dosage of aqueous extract and alcohol extract of Jianhua could significantly increase phenol red excretion in the respiratory tract of mice( P 0. 05,P 0. 01); high-dosage of aqueous extract and alcohol extract of Jianhua could significantly prolong the incubation of asthma in guinea pigs induced by citric acid( P 0. 05). [Conclusions]The extract of Jianhua had significant effect of relieving a cough,eliminating phlegm and relieving asthma.     

山药总皂甙的提取研究

以山药中总皂甙提取率为考察指标,应用单因素试验和正交设计对山药总皂甙提取工艺中的浸提温度、乙醇的体积分数、浸提时间、料液比进行了研究。以薯蓣皂甙为标准,香草醛—高氯酸为显色剂,冰醋酸为溶剂,用分光光度计在波长为544nm处对山药总皂甙的含量进行了测定,得出山药总皂甙的最佳提取工艺条件是:浸提温度为60℃,浸提时间为6h,乙醇体积分数为80%,料液比为1∶8。     

Preventive Effects of Vitamin C And Polysaccharides in Dioscrea pposite Thunb. on Cadmium-induced Liver Injury in Mice

[Objective]To investigate the preventive protective effects and its mechanism of vitamin C and polysaccharide in Dioscrea pposite Thunb. on cadmium-induced liver damage in mice. [Methods]50 Kunming mice were randomly divided into control group,model group,D. pposite polysaccharides protection group,vitamin C protection group,D. pposite polysaccharides and vitamin C protection group. At the 7th day of feeding,the mice were given saline by gavage,and the mice in other groups were intraperitoneally injected with 3. 0 mg /kg CdCl2 solution. 24 hours after injection,blood was collected for ALT and AST test. Then,mice were sacrificed and the livers were removed for the calculation of hepatosomatic index(liver weight / body weight). Also,the liver homogenates were prepared for the determination of SOD and GSHPx activities and the of MDA and NO contents. [Results]Compared with the mice in model group,the liver-somatic index,serum ALT,AST activity,and contents of liver MDA and NO reduced,the activities of SOD and GSH-Px,and the content of GSH improved in D. pposite polysaccharides and vitamin C protection group(*P 0. 05,**P 0. 01). Compared with the model grfoup,the protective effects of D. pposite polysaccharides combined with vitamin C strengthened(**P 0. 01). [Conclusions]The combination of D. pposite polysaccharides and vitamin C has preventive protection effects on cadmium-induced liver injury in mice by antagonizing cadmium and /or oxidative stress. The application of D. pposite polysaccharides combined with vitamin C had a synergistic effect.     

葱乙醇提取物对小鼠免疫功能的影响

摘 要：【目的】本研究利用流式细胞术、DTH法和LDH法探讨葱乙醇提取物对小鼠特异性和非特异性免疫的影响,为调节动物免疫状态和改善机体防御能力提供试验依据。【方法】将昆明种小鼠随机分为环磷酰胺组（C）、环磷酰胺+葱乙醇提取物组（C+A）、葱乙醇提取物组（A）和生理盐水组（S）。环磷酰胺皮下注射造成小鼠免疫抑制;C+A 组和A组给小鼠葱乙醇提取物连续灌胃35 d;S组灌服等量生理盐水。观察测定临床症状、免疫器官指数、细胞免疫功能、体液免疫功能和非特异性免疫功能。【结果】结果显示,葱乙醇提取物可以提高免疫器官指数;对免疫抑制小鼠的左右耳重量差、CD4+/CD8+值、血清抗体和巨噬细胞吞噬活性都有促进作用,对正常小鼠的腹腔巨噬细胞吞噬活性和NK细胞活性有促进作用。【结论】结果表明,葱乙醇提取物对小鼠的特异性免疫和非特异性免疫功能具有显著的调节作用。     

Study on Screening of Anti-inflammatory Activity Areas in the Stems and Leaves of Platycodon grandiflorum

[Objective]To study anti-inflammatory effects of water extract of the stems and leaves of Platycodon grandiflorum. [Methods]Observed anti-inflammatory effects of extract of the stems and leaves of Platycodon grandiflorum using 4 kinds of animal models of acute inflammation which was consisted of rats footpad swelling induced by egg white,ear swelling caused by xylene in mice,celiac capillary permeation in mice induced by 1% glacial acetic acid and content of PGE2in inflammatory tissue of rats' swelling foot induced by egg white. [Results]Extract of the stems and leaves of Platycodon grandiflorum had a certain degree inhibition effect on ear swelling caused by xylene in mice,rats' footpad swelling and content of PGE2in inflammatory tissue of rats' swelling foot induced by egg white and celiac capillary permeation in mice induced by acute inflammation. Compared with model group,parts of the stems and leaves of Platycodon grandiflorum that contained alkaloid were of significant differences( P 0. 05 or P 0. 01). [Conclusions] Parts of the stems and leaves of Platycodon grandiflorum that contained alkaloid had strong anti-inflammatory effects and other parts also had various degrees of anti-inflammatory effects.     

Systematic Preliminary Test on the Chemical Components of Tibetan Herb of Leontopodium franchetii Beauv.

[Objective]To carry out systematic preliminary test on the chemical components of Tibetan herb of Leontopodium franchetii Beauv.[Methods]Systematic preliminary test was adopted; physical and chemical identification of the water extracting solutions,ethanol extracting solutions and petroleum ether extracting solutions were carried out for L. franchetii. The chemical components were preliminarily identified and analyzed. [Results] There might have flavonoids,organic acids,alkaloids,cardiac glycosides,saponins,anthraquinones,phenols,tannins,coumarins,lactones and other components in L. franchetii. [Conclusions] It was preliminarily identified that there were various chemical components in L. franchetii,which provided experimental foundation for the bioactive components and drug efficacy research of L. franchetii.     

水茄叶片总皂苷超声提取工艺的研究

为提高水茄叶片总皂苷的提取率,本试验优化水茄总皂苷的提取工艺。以水茄叶片总皂苷提取率为评价指标,通过单因素试验考察乙醇浓度、料液比、提取时间和提取温度对提取率的影响;在单因素试验的基础上,进行响应面试验设计,对水茄叶片总皂苷的超声辅助提取工艺进行优化。结果表明,最佳工艺条件为乙醇浓度80.30%,料液比1:15.06 (g/mL),提取时间95.56 min,提取温度62.29℃,在此条件下,水茄叶片总皂苷的理论提取率为38.82%,且响应面模型拟合性良好、预测性良好、操作可信。响应面法优化的水茄叶片总皂苷超声提取工艺稳定可行。     

The Antioxidation of Proanthocyanidins from in Rhodiola rosea L.

[Objective]To discuss the antioxidation of proanthocyanidins( OPCRR) in Rhodiola rosea L. [Methods]By establishing peroxide injury model of ICR mice induced by D-gal,intragastric administration of mice was carried out for continuous 49 d. Then,80,160 and 320( BW) mg/kg OPCRR were added into low-,middle- and high-dosage groups,respectively. The SOD,GSH,GSH-Px and MDA in serum,heart,liver and brain tissues and the MAO in liver and brain tissues were investigated. [Results] Compared with model control group,OPCRR at certain dosage could significantly enhance the SOD and GSH-Px activities of mice serum,heat,liver and brain tissues,and obviously reduced the MDA content. And high-dosage group was the most prominent. In high-dosage group,SOD activities of serum,heart,liver and brain tissues enhanced by 29. 5%,28. 1%,62. 7% and 90. 8%,respectively; GSH-Px activities enhanced by 67. 8%,58. 4%,32. 1% and50. 3%,respectively; MDA contents reduced by 19. 2%,30. 0%,33. 3% and 19. 2%. Besides,middle- and high-dosage groups showed extremely significant effects on reducing the brain MAO activity( P 0. 01),which decreased by 22. 3% and 28. 6%,respectively. High-dosage group significantly reduced the liver MAO activity( P 0. 05) by 36. 5%. [Conclusions] OPCRR protected the organism in different degrees,antagonized peroxidation injury induced by D-gal,and prevented from aging.     

半夏乙醇提取物对小菜蛾幼虫生物活性的研究

摘 要：【目的】为了有效控制小菜蛾的危害,研发新型、高效植物源杀虫剂。【方法】采用95%乙醇对半夏干粉进行索式提取,以叶片浸渍法和浸虫法测定了提取液对小菜蛾幼虫的杀虫活性。【结果】 半夏乙醇提取物对小菜蛾3龄幼虫具有较好的拒食、触杀、胃毒和生长抑制的作用,提取物浓度越高,效果越明显。在触杀试验中,当提取物在100 mg/ml浓度下,处理72h后小菜蛾幼虫的校正死亡率为61.44%。在选择性拒食试验中,提取物对3龄幼虫的AFC50分别为32.68 mg/ml（24h）和39.16 mg/ml (48h) ;在非选择性拒食试验中,提取物对3龄幼虫的AFC50分别为17.26 mg/ml（24h）和21.32 mg/ml (48h)。半夏乙醇提取物对小菜蛾的胃毒及生长发育抑制作用也明显。饲喂72 h时,浓度为100 mg/ml处理的小菜蛾幼虫校正死亡率高达69.41%;在处理48 h,浓度为100 mg/ml的提取物对小菜蛾3龄幼虫的生长抑制率为64.43%。【结论】半夏提取物对小菜蛾幼虫具有较高的生物活性和理想的控制效果。     

Evaluation of Safety Profile of Jin Weng Zhi Li Extract Used in the Target Animal Chickens

[Objective] to explore and evaluate the safety of Jin Weng Zhi Li Extract on chickens. [Methods]60 healthy chickens( 3 weeks old) from Rongchang were grouped into four groups,i. e.,blank group,one time the recommended dose group( 0. 4 g / kg),three times the recommended dose group( 1. 2 g / kg),five times the recommended dose group( 2. 0 g / kg). Chickens in blank group were fed with normal saline by gastric infusion( 0. 5 mL per chicken),and chickens were fed with Jin Weng Zhi Li extract solution by gastric infusion,2 times a day,14 consecutive days. During the experiment,the clinical manifestation of chickens were observed,the three major physiological indexes( breathing,pulse,body temperature),daily weight gain,blood routine examination and blood biochemical indexes were determined. The chickens used were dissected at the end of the experiment. Their internal organs were observed to determine if there're macroscopic lesions,and the organs were made into paraffin sections. Then histological observation of the paraffin sections was performed. [Results] The three major physiological indexes,blood routine examination and blood biochemical indexes were all within normal range and of no significant difference( P 0. 05); and no pathological lesions were found by observing the tissue section. [Conclusions]The experimental results indicated that the effect of Jin Weng Zhi Li Extract on the clinical manifestation,haematology and histology was within normal range,and it can be used clinically,and the dose of 1 time the recommended dose and 5 times the recommended dose was within the safe dose range.     

Comparative Research of the Chemical Components of RADIX at RHIZOMA CYATHULAE CAPITATAE with RADIX et RHIZOMA CYATHULAE OFFICINALIS and RADIX et RHIZOMA ACHYRANTHIS BIDENTATAE by Systematic Preliminary Test

[Objective]To carry out the systematic preliminary test on the chemical components of RADIX at RHIZOMA CYATHULAE CAPITATAE,and to compare with RADIX et RHIZOMA CYATHULAE OFFICINALIS and RADIX et RHIZOMA ACHYRANTHIS BIDENTATAE.[Methods]Systematic preliminary tests was adopted;physical and chemical identification of the water extracted solutions,ethanol extracted solutions and petroleum ether extracted solutions were carried out for RADIX at RHIZOMA CYATHULAE CAPITATAE,RADIX et RHIZOMA CYATHULAE OFFICINALIS and RADIX et RHIZOMA ACHYRANTHIS BIDENTATAE,respectively.The chemical components were preliminarily identified and compared.[Results]The three medicines all had saccharides,glycosides,proteins,saponins,phenols,organic acids,flavonoids,alkaloids,sterides,triterpenes and so on.Hydrochloric acid-magnesium powder test on the identification of flavonoids showed that the three medicines were all negative,indicating that the structures of flavonoids components were similar.Alkali test on the anthraquinones identification showed that only RADIX at RHIZOMA CYATHULAE CAPITATAE was negative;identification test on volatile oil and grease showed that only RADIX et RHIZOMA ACHYRANTHIS BIDENTATAE was positive.[Conclusions]It was preliminarily identified that there were saccharides,glycosides,proteins,saponins,phenols,organic acids,flavonoids,alkaloids,sterides and triterpenes in RADIX at RHIZOMA CYATHULAE CAPITATAE.     

Detection of SO_2 Residual in FLOS CHRYSANTHEMI by Accelerated Solvent Extraction/Ion Chromatography

[Objective] To discuss the detection method for SO2 residual in FLOS CHRYSANTHEMI. [Methods] Accelerated Solvent Extraction / Ion Chromatography was used to detect the SO2 residual in FLOS CHRYSANTHEMI. Alkaline solution was used as the extracting solution of grinded sample. After extraction by accelerated solvent extraction apparatus and purification by C18 solid phase extraction,the sample was isolated by IonP ac AS9- HC ion chromatographic column with carbonate as the leacheate. Detection was carried out by electrical conductivity detector. [Results] SO2 had good linear relationship within the range of 0. 5- 50 mg / L( r2= 0. 999 7). The quantitative lower limit( LOQ) and and detection limit( LOD) were 0. 17 and 0. 05 mg/L,respectively. With blank sample as the matrix,the average recovery rates at high,middle and low dosages were 82. 9%- 92. 6% with RSD( n = 6) less than 5. 0%. [Conclusions]Compared with the traditional method,this method had the advantages of high extraction rate and automation degree,and was suitable for the large-scale detection of SO2 residual in FLOS CHRYSANTHEMI.