Study on the Lactation-promoting Function Experiment of Yue-naitang Decoction in Pregnant Female Rats

[Objective]To investigate the lactation-promoting function of Yue-nai-tang decoction in female rats.[Methods]The newly delivered female rats were divided into 6 groups,blank control,low dosage group(125 mL/kg),moderate dosage group(250 mL/kg),high dosage group(500 mL/kg),pure rice wine group(250 mL/kg)and Muruduo granules as positive control group(9 g/kg).Each group contained8 female rats.Each fed 10 newborn rat pups to simulate the overload lactation model.Medication was lasted continuously for 21 days by intragastric administration.The growth status of rats was observed daily by recording the lactation of each female rat and body weight of cub.After medication,mammary gland of experimental rats was taken out for determining the mammary gland index and observing mammary sections.[Results]Both the lactation and body weight of rats in moderate dosage group and high dose group were remarkably higher than that in blank control,while did not vary significantly from positive control group.The mammary section observation found that the adenosis of mammary gland of female rats in middle and high dosage group(250,500 mL/kg)grew normally,with no difference from mammary gland of normal lactation rats,significantly superior to blank control and pure rice wine group,while not different from the positive group.[Conclusions]Yuenai-tang decoction can promote the lactation of newly delivered rats,thus facilitating the growth of mammary gland and cubs.     

Effects of Water Decoction of Plumbago zeylanica L. on the Proliferation,Apoptosis and Cell Cycle of Rats Hepatic Stellate Cells

[Objective] To observe the effects of water decoction of Plumbago zeylanica Linn.on the proliferation,apoptosis and cell cycle of rats hepatic stellate cells,and to discuss the function and mechanism of anti-hepatic fibrosis of P.zeylanica.[Methods] SD rats were given water decoction of P.zeylanica by gavage,so as to obtain medicated serum.Medicated serum was incubated together with hepatic stellate cells( HSC-T6) according to different dosage groups.Blank control group was desgined; and medicated serum groups of colchicine and Compound Biejiaruangan Tablet were taken as the positive control groups.Proliferation of HSC-T6 was detected after incubation by MTT colorimetry; cell apoptosis and the DNA content in each cell phase was detected by flow cytometer.[Results]Inhibitory rate and apoptosis rate: compared with blank control,inhibitory rate and apoptosis rate in medicated serum groups enhanced significantly,showing significant differences( P 0.01).When the dosage of medicated serum was within 5%- 20%,inhibitory rate and apoptosis rate of HSC-T6 enhanced as dosage increased.Inhibitory rate and apoptosis rate in medicated serum group of high dosage were significantly higher than those in colchicine groups,but was equal to those in Compound Biejiaruangan Tablet group.Cell cycle: there were no significant changes in cell percentage in each group at G2/M phase.Compared with blank control group,cell percentages of medicated serum groups of P.zeylanica enhanced significantly at G0/G1 phase,and reduced significantly at S phase,showing significant changes( P 0.01).When the dosage of medicated serum reduced within 20%- 5%,the cell percentage gradually declined at G0/G1 phase,and gradually increased at S phase.Under the same serum concentration,cell percentage of P.zeylanica group enhanced significantly at G0/G1 phase and decreased obviously at S phase compared with that of colchicine group.There were no significant changes in Compound Biejiaruangan Tablet group.[Conclusions] Medicated serum of P.zeylanica could restrict G1/S proliferation and induce its apoptosis,showing a dose-dependent manner.Medicated serum group of P.zeylanica had stronger effects than medicated serum group of colchicine,while had equal effects to the group of Compound Biejiaruangan Tablet.The mechanism of medicated serum of P.zeylanica in inhibiting HSC-T6 proliferation was to block cell cycle at G0/G1 phase,and to prevent it from passing G1/S.     

Evaluation of Safety Profile of Jin Weng Zhi Li Extract Used in the Target Animal Chickens

[Objective] to explore and evaluate the safety of Jin Weng Zhi Li Extract on chickens. [Methods]60 healthy chickens( 3 weeks old) from Rongchang were grouped into four groups,i. e.,blank group,one time the recommended dose group( 0. 4 g / kg),three times the recommended dose group( 1. 2 g / kg),five times the recommended dose group( 2. 0 g / kg). Chickens in blank group were fed with normal saline by gastric infusion( 0. 5 mL per chicken),and chickens were fed with Jin Weng Zhi Li extract solution by gastric infusion,2 times a day,14 consecutive days. During the experiment,the clinical manifestation of chickens were observed,the three major physiological indexes( breathing,pulse,body temperature),daily weight gain,blood routine examination and blood biochemical indexes were determined. The chickens used were dissected at the end of the experiment. Their internal organs were observed to determine if there're macroscopic lesions,and the organs were made into paraffin sections. Then histological observation of the paraffin sections was performed. [Results] The three major physiological indexes,blood routine examination and blood biochemical indexes were all within normal range and of no significant difference( P 0. 05); and no pathological lesions were found by observing the tissue section. [Conclusions]The experimental results indicated that the effect of Jin Weng Zhi Li Extract on the clinical manifestation,haematology and histology was within normal range,and it can be used clinically,and the dose of 1 time the recommended dose and 5 times the recommended dose was within the safe dose range.     

热应激小鼠附睾组织HSP70表达的研究

利用化学恒温培养箱对小鼠实施42℃1h/d的热应激处理,建立持续的全身热应激动物模型。在热应激持续到8d、13d、21d和35d时,颈椎脱臼处死对照组和热应激组小鼠,应用改良巴氏染色法检测附睾精子畸形率,免疫荧光组织化学和蛋白质印迹分析的方法检测附睾组织HSP70的表达。结果显示：热应激组小鼠附睾精子畸形率随着热应激持续时间的延长而升高,且显著（P<0.05）高于对照组;对照组小鼠附睾组织HSP70呈极强表达（+++）,而热应激组小鼠附睾组织HSP70的表达随着热应激持续时间的延长而减弱,当热应激持续到35d时呈极弱的表达（±）。结果表明：热应激损伤了附睾的功能,使附睾精子畸形率增加;附睾内HSP70为非热诱导型,HSP70可能在附睾精子的成熟过程发挥特殊的作用。     

银芩麻杏石甘汤与桔芪麻杏石甘汤抗山羊支原体肺炎研究

为了研究方剂对羊支原体肺炎的防治效果,将感染山羊支原体前后的羊采集静脉血,分离血清,用ELISA方法检测IL-10、IFN-γ和TNF-α含量。同时,每日观察临床症状,对迫杀的试验羊进行病理剖检和组织切片观察。结果显示：正常对照组未见异常,阳性对照组症状明显,死亡4只,方剂治疗组均比阳性对照组临床症状有明显的改善,银芩麻杏石甘汤中剂量组全部治愈。7天时阴性对照组与其他7组之间IL-10、IFN-γ和TNF-α差异均极显著(P<0.01);14天时阴性对照组与其他7组差异极显著(P<0.01),阳性对照组与6组中药组差异极显著(P<0.01),银芩中剂量组与其他5组中药组差异显著(P<0.05);21天时IL-10、IFN-γ阴性对照组与其他7组差异极显著(P<0.01),阳性对照组与6组中药组差异极显著(P<0.01),银芩中剂量组与其他5组中药组差异极显著(P<0.01),银芩中剂量组与阴性对照组差异不显著(P>0.05)。21天时TNF-α阴性对照组与其他7组差异极显著(P<0.01),阳性对照组与6组中药组差异极显著(P<0.01),桔芪高剂量组与其他5组中药组差异极显著(P<0.01),桔芪高剂量组与阴性对照组差异不显著(P>0.05)。由结果得到2个方剂均对治疗羊支原体肺炎有一定的效果,银芩麻杏石甘汤中剂量组对羊支原体肺炎的治疗效果最佳。     

瓦松乙醇提取物降血糖的试验研究

观察瓦松乙醇提取物对链脲佐菌素(STZ)诱发的糖尿病大鼠的降血糖作用。采用腹腔注射STZ引起糖尿病动物模型,分别以瓦松乙醇提取物低、中、高剂量灌喂28d,给药期间每隔7d测定一次空腹血糖值,给药结束后称量体重及进行经口糖耐受量试验。结果显示,瓦松提取物能有效降低糖尿大鼠的空腹血糖值,改善经口糖耐受能力,并有一定的量效和时效关系。     

甘蓝型油菜热激蛋白HSP70基因家族的鉴定和分析

热休克蛋白也称热激蛋白(heat shock protein, HSP),是植物在受到生长环境中不利的生长因子胁迫时产生的一种用于抗逆的防御蛋白,其中在生物体中分布最普遍的一种热激蛋白是热休克蛋白70 (heat shock protein 70, HSP70)。为全面、深入地了解芸薹属HSP70基因家族的信息,本研究对芸薹属的3个物种(白菜,甘蓝和甘蓝型油菜)进行了生物信息学分析,首先在BRAD数据库中对芸薹属的3个物种进行HSP70基因数目的鉴定,分别在白菜和甘蓝中鉴定出了2个HSP70,在甘蓝型油菜中鉴定出了4个HSP70。将鉴定出来的这8个HSP70基因进行生物信息学分析(系统发生关系,基因结构,保守基序,染色体定位和同源性等),结果显示,芸薹属HSP70基因结构相似,在核酸和蛋白水平均具有高度保守性,进化过程中基本种之间以及天然杂交种与基本种之间均保持着极高的同源性,进化树以及其他生物信息学分析结果还表明甘蓝型油菜的HSP70位点与白菜、甘蓝的HSP70位点符合"禹氏三角"理论,但是根据3个物种的HSP70基因数量及对应关系等分析结果可推测白菜、甘蓝、甘蓝型油菜3个物种的HSP70位点与三倍化理论不完全一致,此结果可反映芸薹属的这3个物种在进化过程中其HSP70基因在数量或结构上发生过变化,致使其不满足三倍化学说。     

奶牛HSP 70表达量与温湿指数的相关性研究

利用免疫学和分子生物学手段研究荷斯坦牛和娟-荷F1牛,在环境温湿指数(THI)影响下,血液淋巴细胞热应激蛋白70(HSP 70)的表达情况,旨在探索HSP 70表达量与THI相关性以及不同品种差异性。结果表明：(1)荷斯坦成乳牛在热应激和非热应激条件下均可表达HSP 70,严重热应激期(THI 93.5±2.1)HSP 70表达量分别是中度热应激期(THI 76.7±2.6)1.9倍(P<0.01)和非热应激期(THI 56.5±1.7)6.9倍(P<0.01),中度热应激期HSP 70表达量是非热应激期3.6倍(P<0.05),HSP 70表达量与THI呈强度正相关(R=0.91)(P<0.01),THI每上升1个单位,HSP 70表达量增加2172 OD/mm2;(2)在严重热应(THI 95.3)条件下,娟-荷F1成乳牛HSP 70表达量极显著高于荷斯坦牛(P<0.01)。 关键词：奶牛;热应激蛋白;温湿指数;相关性     

黑苦荞胶囊对四氧嘧啶糖尿病小鼠的降血糖作用

评价黑苦荞胶囊降血糖功能,为黑苦荞保健食品开发提供依据。采用腹腔注射四氧嘧啶建立糖尿病小鼠动物模型。将试验动物分为模型对照组,低、中、高剂量组,同时设正常动物的空白对照组和受试样品组。经口给予黑苦荞胶囊,连续灌胃30 d,观察其对小鼠体重、空腹血糖及糖耐量的影响。结果表明,黑苦荞胶囊对血糖正常组小鼠的体重及空腹血糖值无显著的影响(p>0.05)。高血糖模型各剂量组小鼠空腹血糖值与模型对照组相比均呈现显著下降(p<0.05),糖耐量试验中,低剂量组和中剂量组血糖曲线下面积显著降低(p<0.01,p<0.05)。黑苦荞胶囊对四氧嘧啶致糖尿病小鼠具有降血糖功能。     

Effects of Yuezhitang Decoction on Lactation Promotion of Over Lactation Model Mice

[Objective] To discuss the effects of Yuezhitang decoction on promoting lactating function of maternal mice,enhancing the quality of newborn mice,and improving the survival ratio of newborn mice by using the overload lactation model of mice.[Methods]Overload lactation model of maternal mice was established.Maternal mice were given different dosages of samples by gavage.The lactation volume,accumulative lactation volume,body weight change of newborn mice at lactation period were researched.The mammary gland index was detected; the changes of breast tissue morphology and physiology were observed.[Results] Compared with model group,11 and 16.5 g/kg dosage groups significantly improved the lactation volume of maternal mice(P0.05).Mammary gland index of 11 g/kg dosage group increased significantly.In 11 and 16.5 g/kg dosage groups,mammary gland acinus had dense structure; the acinus was arranged regularly with complete structure and significant increase in quantity,showing typical characters of secretory mammary gland.[Conclusions]Yuezhitang decoction promoted the lactation of maternal mice.     

金花葵黄酮对糖尿病模型小鼠脂代谢的影响

观察金花葵总黄酮对糖尿病模型小鼠脂代谢以及SOD的影响,将链脲佐菌素(STZ)诱导成功的糖尿病小鼠模型随机分为正常对照组、金花葵黄酮高剂量组,中剂量组和低剂量组,阴性对照组和阳性对照组。灌胃4周后检测血脂和超氧化物岐化酶(SOD)的变化。结果表明,与阴性对照组相比,金花葵黄酮干预小鼠总胆固醇下降（P<0.01）,高密度脂蛋白胆固醇上升（P<0.05）,SOD上升（P<0.01）。适量金花葵黄酮对糖尿病小鼠脂代谢紊乱有明显的改善作用,并有显著的体内抗氧化能力。     

The Acute Toxicity and Subchronic Toxicity Test of Zhuqin Extract

[Objective] To investigate the safety of compound Zhuqin extract.[Methods]In acute toxicity test,mice in test group were given compound Zhuqin extract( i. g.) by gavage,the dosage of which was 0. 8,0. 6,0. 45,0. 35 g /20 g,to determine the median lethal dose of compound Zhuqin extract( LD50). In maximum tolerance experiment,mice in test group were given compound Zhuqin extract( i. g.) by gavage,the dosage of which was 0. 5 g /20 g,to determine the maximum tolerance dose( MTD); In the sub- chronic toxicity test,rats in test group were given compound Zhuqin extract( i. g.) by gavage for 30 d,the dosage of which was 2. 0,1. 5,1. 0 g /200 g,and normal saline negative control group was set. Clinic condition of rat was observed everyday,hematology,blood biochemistry,anatomy,organ index and organization pathological observation were conducted after test.[Results]The acute toxicity test failed to detect median lethal dose of compound Zhuqin extract; the weight differences were not significant( P 0. 05); some blood biochemical criterion and blood routine index had significant differences( 0. 01 P 0. 05),and some indexes had extremely differences( P 0. 01); organ index,necropsy and histopathology had abnormalities.[Conclusions] Compound Zhuqin extract was safe and reliable.     

BnA7HSP70分子伴侣结合蛋白超表达能够提高甘蓝型油菜耐旱性

分子伴侣结合蛋白广泛参与植物生长发育过程, 在逆境下能够保护植物细胞免受胁迫。在甘蓝型油菜中超表达油菜含有HSP70热激蛋白结构域的分子伴侣基因BnA7HSP70, 所得到的转基因植株在缺水条件下延缓萎蔫。通过生理生化实验证明, 干旱条件下转基因植株有着更高的相对含水量、更强的渗透调节能力和较低的脂质膜过氧化性。另外, 转基因植株的幼苗在萌发期表现出对糖基化酶抑制剂衣霉素处理的耐受性。Evans blue染色实验证明, 转基因植株逆境下叶片死亡细胞数目比非转基因植株减少, 叶片衰老相关标记基因BnCNX1在转基因植株中下调表达证明, 超表达BnA7HSP70基因所介导的途径能够减轻逆境胁迫下的植株衰老, 保持叶片持绿性。在转基因植株中内质网和渗透胁迫产生的细胞死亡标记基因N-Rich蛋白BnNRP延迟表达证明, 在油菜中增强BnA7HSP70基因的表达能够缓解未折叠蛋白途径(unfold protein response, UPR)和NRP (N-rich pathway)途径介导的叶片黄萎, 并降低油菜叶片失绿的标记基因BnLSC222和BnLSC54的表达。研究结果表明, 在油菜中超表达BnA7HSP70基因能够提高植株在干旱条件下内质网胁迫的耐受性。     

玉米须醇提物对链脲菌素诱导糖尿病小鼠的血糖影响研究(英文)

玉米须(Maydisstigma)是具有多种生理功能的传统中药,首次对玉米须醇提物结合链脲菌素诱导的糖尿病ICR小鼠分别设计了短期单次灌胃试验和长期灌胃试验。以格列苯脲(Glibenclamide)为阳性对照,测定玉米须对模型小鼠基础血糖水平、糖耐量和内源胰岛素含量,以及体质量等指标的变化影响。短期试验结果显示,玉米须可以降低基础血糖水平和抑制外源葡萄糖刺激下的血糖升高。4周长期灌胃试验中,玉米须和Glibenclamide使糖尿病小鼠血糖明显下降,内源胰岛素含量在Glibenclamide作用下升高,而玉米须没有这样的效果,但玉米须对糖尿病小鼠体质量下降有缓解作用。研究结果首次揭示桔梗具有降低血糖效果,但对内源胰岛素分泌没有影响。     

棉花HSP70基因植物表达载体的构建及转化烟草的研究

为研究棉花HSP70 基因在转基因植物中抗旱效果,利用前期克隆的GhHSP70 基因,以pCAMBIA1304 质粒为植物表达载体,构建了GhHSP70 基因的植物表达载体CAMBIA1304-HSP70;采用冻融法转入根癌农杆菌EHA105 菌株,通过叶盘法对模式植物烟草进行遗传转化研究。结果表明,在潮霉素(50 mg/L)选择压力下获得的烟草转化不定芽和完整植株,经过PCR方法以及GUS基因组织化学法检测鉴定转基因烟草,其中有5 株为阳性植株。初步证实了GhHSP70 基因已导入烟草基因组中。为进一步研究该基因的功能提供实验基础,同时为后续转化棉花改善其抗旱能力奠定基础。     

Hypoglycemic Effects of the n-Butanol Part of Sorbus tianschanica

[Objective] To extract and isolate the effective components from Sorbus tianschanica,and to research the hypoglycemic effects of n-Butanol part. [Methods]S. tianchanica was extracted by reflux extraction. After gradient elution,n-Butanol part was obtained. With diabetic mice induced by STZ as the model,blood glucose test was carried out. [Results]n-Butanol part in administration group had significant differences in hypoglycemic effects with that in model group(P 0. 05),showing that n-Butanol part had certain antidiabetic functions on diabetic mice induced by STZ. And the hypoglycemic effects showed a reducing trend as the administration dosage decreased,indicating that administration dosage had positive correlation with hypoglycemic effects. n-Butanol in different parts could reduce the blood glucose of diabetic mice in different degrees. And the blood glucose value in high-dosage group(8 g /kg) reduced greatly at 2 h after administration,having great differences with that in model group(P 0. 01). This showed that high dosage was helpful to the glucose tolerance of diseased mice,and could effectively protect against the rapid increase of blood glucose after meal. n-Butanol had no significant effects on the blood glucose of normal mice. However,the morality rates in high-and middle-dosage groups were both higher than that in low-dosage group; and the morality rate in administration group was lower than that in model group,showing that n-Butanol could promote the survival rate of diabetic mice. [Conclusions]S. tianchanica had certain effects of anti-diabetes.     

The Effect of Arbutin on Mice Brain Cells after Ischemia-reperfusion Injury

[Objective]To study the effect of the Chinese traditional medicine arbutin on brain cells after ischemia-reperfusion injury and provide a theoretical foundation for its clinical application. [Methods]Forty Kunming mice were randomly divided into four groups,namely the blank group,the ischemia-reperfusion group,the chemoprophylaxis group and the medication group. The cerebral ischemia-reprefusion model,made according to the principle of brain damage caused by ischemia,was stained with TTC and HE to observe cells' morphological changes.SOD activity,MDA content and GSH-Px activity in brain tissues were tested. [Results]Compared with the ischemia-reperfusion group,necrosis of ischemia areas stained by TTC in the chemoprophylaxis group and the medication group was less obvious. HE staining showed the alleviation of cells damage. The enhancement of SOD activity and that of GSH-Px activity showed significant differences. MDA content was reduced(P 0.05 for both). [Conclusions]Arbutin had antioxidant properties which can effectively prevent and protect brain cells from damage.     

Anti-bacterial Effects in Vitro of Jinweng Zhili Granules and Its Antipyretic and Anti-inflammatory Effects on Animal

[Objective] To research the anti-bacterial effects in vitro of Jinweng Zhili Granules and its antipyretic and anti-inflammatory effects on animal. [Methods]Anti-bacterial effects of Jinweng Zhili Granules were detected by MIC method and drug sensitive test. A total of60 male Kunming mice were randomly divided into blank control group,aspirin group,low-dosage group of Jinweng Zhili Granules,middledosage group of Jinweng Zhili Granules,and high-dosage group of Jinweng Zhili Granules. Mice were given drugs by gavage for 7 d. Swelling degree of ear slice was calculated and the anti-inflammatory effects were analyzed. A total of 60 rabbits were randomly divided into high-dosage group of Jinweng Zhili Granules,middle-dosage group of Jinweng Zhili Granules,low-dosage group of Jinweng Zhili Granules,positive drug group,blank control group and model group. Endotoxin was administrated through the ear venous in order to establish febrile model. Effects of Jinweng Zhili Granules on the body temperature of febrile rabbits induced by endotoxin were analyzed. Blood was collected by heart blood collection method. When the plasma was separated,cA MP content in rabbits were detected. [Results] The MIC values to Escherichia coli,Staphylococcus aureus,Clostridium welchii,Salmonella typhimurium,Candida albicans were 3. 75,1. 875,15,7. 5 and 7. 5 mg / mL,respectively. The swelling degree of mice slice in model group reached 121. 7%. And the swelling degrees in high- and middle-dosage groups of Jinweng Zhili Granules were 55. 6% and 56. 2%,respectively,which had extremely significant differences with that of model group( P 0. 01).Jinweng Zhili Granules reduced the body temperature of febrile rabbits induced by endotoxin. The cA MP content in model group was 34. 13 pmol / mL; and the cA MP contents in high-and low-dosage groups were 24. 74 and 26. 61 pmol / mL,respectively,which had extremely significant differences compared with model group( P 0. 01). [Conclusions]Jinweng Zhili Granules had anti-bacteria,anti-inflammation and antipyretic effects.     

藿芪灌注液毒性研究

为了测定小鼠口服藿芪灌注液的急性毒性和长期毒性,评价其安全性,为临床安全用药提供依据,采用最大给药量法进行了急性毒性研究,选择健康小鼠20只,24 h内按40 g/kg体重灌胃给药3次,给药后连续观察10天,测定其1日最大给药量,确定藿芪灌注液的急性毒性。将80只健康大鼠随机分为高、中、低剂量组和对照组,每组雌雄各10只,高、中、低剂量组分别按20、10、3 g/kg体重灌服藿芪灌注液,对照组按20 mL/kg灌服生理盐水,每天一次,连续给药35天,通过临床观察、病理组织学检查、血液生理生化指标测定,研究其长期毒性。结果表明,小鼠口服藿芪灌注液的1日最大给药量为120 g/kg,相当于临床奶牛日用量的600倍。藿芪灌注液不影响大鼠的采食、活动、饮水、增重、脏器指数、血液生理生化指标,不会引起大鼠发病和死亡。说明藿芪灌注液实际无毒,至少在20 g/kg体重给药剂量下大鼠连续给药35天是安全的。     

枳水浸提液对嗜酒大白鼠机能的影响

设计正常饲养、低剂量饲养、高剂量饲养和模型对照4个实验组,研究枳椇水浸提液对嗜酒30 d大白鼠的体质量增加,以及血液中乙醇含量、丙二醛(MDA)含量及乙醇脱氢酶(ADH)活性的影响。试验结果表明,在体质量增加方面,高剂量和低剂量实验组比模型对照组非常显著性升高(p<0.01);在血液中丙二醛(MDA)含量和乙醇脱氢酶(ADH)活性方面,高剂量实验组大白鼠血液中的丙二醛(MDA)含量比对照组非常显著差性降低(p<0.01),而乙醇脱氢酶(ADH)活性比对照组非常显著性升高(p<0.01);在血液中乙醇浓度方面,2个枳椇提取液实验组中大白鼠血液的乙醇含量均低于模型对照组。枳椇水浸提液对大白鼠的长期嗜酒产生的功能损伤具有一定的保护作用。