EVALUATING THE CLINICAL AND PHYSIOLOGICAL EFFECTS OF LONG-TERM ULTRAVIOLET B RADIATION ON RABBITS (ORYCTOLAGUS CUNICULUS)

Vitamin D is an essential hormone in vertebrates. Most animals acquire this hormone through their diet and/or exposure to ultraviolet B (UVB) radiation. The objectives for this research were to evaluate the clinical and physiologic effects of artificial UVB light supplementation on rabbits (Oryctolagus cuniculus) and to evaluate the long-term safety of artificial UVB light supplementation over a 6-month period. Twelve New Zealand white rabbits were randomly assigned to one of two treatment groups: Group A was exposed to 12 hours of artificial UVB radiation daily and Group B received ambient fluorescent light with no UVB supplementation for 12 hours daily. All animals were offered the same diet and housed under the same conditions. Blood samples were collected every 3 weeks over 6 months to measure blood chemistry values, parathyroid hormone, ionized calcium, and serum 25-hydroxyvitamin D₃ (25-OHD₃) levels. Serial ophthalmologic examinations were performed at the beginning of the study and every 2 months thereafter. At the end of the study the animals were euthanized and necropsied. Mean ± SD serum 25-OHD₃ concentrations differed significantly (P = 0.003) between animals provided supplemental UVB radiation (83.12 ± 22.44 nmol/L) and those not provided UVB radiation (39.33 ± 26.07 nmol/L). There were no apparent negative clinical or pathologic side effects noted between the groups. This study found that exposing rabbits to UVB radiation long term significantly increased their circulating serum 25-OHD₃ levels, which was sustainable over time.     

EFFECTS OF NATURAL ULTRAVIOLET RADIATION ON 25-HYDROXYVITAMIN D3 CONCENTRATIONS IN FEMALE GUINEA PIGS (CAVIA PORCELLUS)

Vertebrates have 2 methods of acquiring vitamin D: through the diet and/or secondary to exposure to ultraviolet B (UVB) radiation. Although some species (e.g., dogs) can only acquire vitamin D through their diet, many others also utilize UVB radiation to generate vitamin D. Prior to their extirpation, guinea pigs were naturally exposed to varying levels of sunlight (UVB) in their native habitat; however, in captivity we do not routinely recommend UVB radiation for these animals. Recently, it has been shown that captive guinea pigs can synthesize 25-hydroxyvitamin D (25-OHD₃) after exposure to UVB lightbulbs. However, it is not known how natural sunlight impacts 25-OHD₃ concentrations in this species. The purpose of this study was to determine whether 25-OHD₃ concentrations in female guinea pigs exposed to natural sunlight would increase as a result of UVB exposure. Eight adult female guinea pigs were used for this study. The animals were held indoors during winter months and then placed outside in the spring when temperatures were appropriate. Blood samples were collected before the animals were placed outdoors (baseline) and 30 days after being exposed to natural sunlight. There was a significant difference in 25-OHD₃ concentrations over time (P = 0.006) and values collected after the guinea pigs were housed outdoors were 1.8 times higher than baseline. This study confirmed that female guinea pigs can increase 25-OHD₃ concentrations after exposure to natural sunlight. This suggests that these animals have conserved this pathway despite domestication, and supplementation should be considered to optimize captive guinea pig habitats.     

Effects of ultraviolet radiation on plasma 25-hydroxyvitamin D3 concentrations in corn snakes (Elaphe guttata)

OBJECTIVE: To determine whether corn snakes exposed to UVB radiation have increased plasma 25-hydroxyvitamin D3 concentrations, compared with control snakes. ANIMALS: 12 corn snakes (Elaphe guttata). PROCEDURES: After an acclimation period in individual enclosures, a blood sample was collected from each snake for assessment of plasma 25-hydroxyvitamin D3 concentration. Six snakes were provided with no supplemental lighting, and 6 snakes were exposed to light from 2 full-spectrum coil bulbs. By use of a radiometer-photometer, the UVA and UVB radiation generated by the bulbs were measured in each light-treated enclosure at 3 positions at the basking surface and at 2.54 cm (1 inch) below each bulb surface; the arithmetic mean values for the 3 positions at the basking surface and each individual bulb surface were calculated immediately after the start of the study and at weekly intervals thereafter. At the end of the study (day 28), another blood sample was collected from each snake to determine plasma 25-hydroxyvitamin D3 concentration. RESULTS: Mean +/- SD plasma concentration of 25-hydroxyvitamin D3 in snakes that were provided with supplemental lighting (196 +/- 16.73 nmol/L) differed significantly from the value in control snakes (57.17 +/- 15.28 nmol/L). Mean exposure to UVA or UVB did not alter during the 4-week study period, although the amount of UVA recorded near the bulb surfaces did change significantly. CLINICAL RELEVANCE: These findings have provided important insight into the appropriate UV radiation requirements for corn snakes. Further investigation will be needed before exact husbandry requirements can be determined.     

Vitamin D and Ultraviolet B Radiation Considerations for Exotic Pets

Vitamin D is an essential hormone that regulates many different functions in vertebrates and can have a protective effect against various disease conditions. Providing exotic pets appropriate access to vitamin D, through the diet, ultraviolet B (UVB) exposure, or a combination of both, is important for veterinarians and exotic pet owners to consider. Although it is commonly thought that most animals derive the vitamin D they need through their diet, there are a number of species that appear to benefit more from UVB exposure. In addition, there has been minimal study to investigate appropriate dietary levels of vitamin D for many of our exotic pets. Although the recommendation of providing UVB lighting has been primarily limited to captive reptiles, research with other species (e.g., birds and small mammals) suggests that these animals may also benefit from this type of lighting. However, the provision of UVB is not without its potential side effects. The purpose of this article is to review the important roles of vitamin D in animals, the different methods animals use to acquire this hormone, the potential clinical signs associated with hypovitaminosis or hypervitaminosis D, the role of artificial UVB lighting in the synthesis of vitamin D, and the potential side effects associated with UVB radiation.     

Serum vitamin D,calcium, and phosphorus concentrations in ponies,horses and foals from the United States and Thailand

Vitamin D is essential in calcium and phosphorus regulation, bone physiology, cell proliferation and epithelial integrity. Literature on vitamin D in growing horses is sparse, and the effect of age on vitamin D has not been evaluated in equids in the United States or in tropical countries. The goal of this study was to determine if there was an effect of age on serum 25(OH)D₃ concentrations in equids in the US (Ohio/Kentucky) and Thailand (Chiang Rai and Kanchanaburi) during the same time of the year. Blood samples were collected from healthy ponies (n = 21) and Thoroughbred foals (n = 13), yearlings (n = 10), and horses (n = 20) in Thailand and from Thoroughbred foals (n = 10) and horses (n = 17) in the US. Serum concentrations of 25(OH)D₃, calcium and phosphorus were measured.In both countries, serum 25(OH)D₃ concentrations were lower in foals than in yearlings and adult horses. Serum 25(OH)D₃ concentrations were higher in horses than in ponies in Thailand, but were not different between horses from either country. Calcium concentrations were not different between groups or location. In both countries, phosphorus concentrations were higher in foals than in older groups; however, were not different between ponies and horses. This study shows that independent of geography there are age-related differences in 25(OH)D₃ concentrations in horses and further confirms that 25(OH)D₃ concentrations are lower in horses compared to other species. The information will serve as the basis for future clinical studies and to help understand better the pathophysiology of equine disorders associated with calcium and phosphorus dysregulation.     

The effect of maropitant on intraoperative isoflurane requirements and postoperative nausea and vomiting in dogs: a randomized clinical trial

ObjectiveTo establish if preoperative maropitant significantly reduced intraoperative isoflurane requirements and reduced clinical signs associated with postoperative nausea and vomiting (PONV) in dogs.Study designRandomized clinical trial.AnimalsTwenty-four healthy, client-owned dogs undergoing routine ovariohysterectomy.MethodsPremedication involved acepromazine (0.03 mg kg⁻¹) combined with methadone (0.3 mg kg⁻¹) intramuscularly 45 minutes before anaesthetic induction with intravenous (IV) propofol, dosed to effect. Meloxicam (0.2 mg kg⁻¹) was administered intravenously. Dogs were randomly assigned to administration of saline (group S; 0.1 mL kg⁻¹, n = 12) or maropitant (group M; 1 mg kg⁻¹, n = 12) subcutaneously at time of premedication. Methadone (0.1 mg kg⁻¹ IV) was repeated 4 hours later. Anaesthesia was maintained with isoflurane in oxygen, dosed to effect by an observer unaware of group allocation. The dogs were assessed hourly, starting 1 hour postoperatively, using the short form of the Glasgow Composite Pain Score (GCPS), and for ptyalism and signs attributable to PONV [score from 0 (none) to 3 (severe)] by blinded observers. Owners completed a questionnaire at the postoperative recheck.ResultsOverall mean ± standard deviation end-tidal isoflurane percentage was lower in group M (1.19 ± 0.26%) than group S (1.44 ± 0.23%) (p = 0.022), but was not significantly different between groups at specific noxious events (skin incision, ovarian pedicle clamp application, cervical clamp application, wound closure). Cardiorespiratory variables and postoperative GCPS were not significantly different between groups. Overall, 50% of dogs displayed signs attributable to PONV, with no difference in PONV scores between groups (p = 0.198). No difference in anaesthetic recovery was noted by owners between groups.ConclusionsMaropitant reduced overall intraoperative isoflurane requirements but did not affect the incidence of PONV.Clinical relevanceMaropitant provided no significant benefits to dogs undergoing ovariohysterectomy with this anaesthetic and analgesic protocol, although clinically significant reductions in isoflurane requirements were noted.     

The impact of MK-467 on sedation,heart rate and arterial blood pressure after intramuscular coadministration with dexmedetomidine in conscious cats

Objective

To study the effects of MK-467, a peripheral α₂-adrenoceptor antagonist, on sedation, heart rate and blood pressure after intramuscular (IM) coadministration with 25 μg kg^?1 of dexmedetomidine in cats.

Impacts of UVB provision and dietary calcium content on serum vitamin D3, growth rates,skeletal structure and coloration in captive oriental fire‐bellied toads (Bombina orientalis)

Many amphibian species are dependent on ex situ conservation interventions for their long‐term persistence. However, projects have been jeopardised by husbandry issues involving poor calcium metabolism and nutritional metabolic bone disease (NMBD). Healthy calcium metabolism requires appropriate dietary calcium content and access to vitamin D₃. In many animals, vitamin D₃ can be photobiosynthesised in skin exposed to UVB radiation, as well as extracted from the diet, but the extent of vitamin D₃ photobiosynthesis in amphibians is poorly known. Additionally, prey insects for captive amphibians are deficient in calcium and calcium content must be artificially increased, but the effects of different levels of augmentation and their interaction with UVB exposure are also little understood. We fed captive fire‐bellied toads (Bombina orientalis) with crickets augmented to contain 5% and 10% calcium and housed them with and without UVB exposure. Despite additional dietary vitamin D₃ supplementation, we found that toads exposed to UVB radiation exhibited significantly higher serum vitamin D₃ levels, indicating that this species may partly rely on photobiosynthesis sources of vitamin D₃. These data are the first to show a direct link between UVB exposure and serum vitamin D₃ in an amphibian. We found significant positive effects of UVB exposure and 10% dietary calcium content on skeletal structure, as well as complex interactions between treatments. We also found UVB radiation exposure resulted in more rapid natural coloration acquisition. Together, this indicates that standard calcium plus vitamin D₃ supplementation methods may not fully substitute for UVB exposure and for increased feeder insect calcium content. This may have implications for the success of ex situ amphibian conservation, as well as for the welfare of captive amphibians in general. Our data lend support for the provision of UVB radiation for captive, basking amphibians.     

Growth Performance and Serum Mineral Concentrations of Stocker Calves Grazing Wheat Pastures and Fed Different Sources of Magnesium,

Magnesium supplementation has been shown to benefit feedlot cattle and is typically added at high concentrations to mineral supplements for calves grazing wheat pasture. Two experiments were conducted to determine the effects of Mg-mica supplementation on performance and serum mineral concentrations of stocker calves grazing wheat pasture. Supplemental Mg was provided at 6.1 g/d of supplemental Mg/ animal, Monday through Friday. In Exp. 1, 36 mixed-breed stocker calves (243 ± 3.9 kg BW) were stratified by BW and gender and allocated randomly to one of nine groups of four animals for a 112-d grazing study. Weight gain, shrink percentage, and serum Mg, Ca, K, Cu, and Zn concentrations did not differ (P>0.10) among calves fed either weathered Mg-mica (WMM), unweathered Mg-mica (UMM), or MgO. All serum mineral concentrations were within normal physiological ranges. In Exp. 2, 64 mixed-breed stocker steers (275 ± 1.7 kg BW) were stratified by BW and allocated randomly to one of 16 groups of four animals. Weight gain during a 50-d grazing study and subsequent feedlot period did not differ (P>0.10) among calves fed either no added Mg source or WMM, UMM, or MgO. Serum Cu was higher (P<0.05), and serum Mg was lower (P<0.05), from steers fed MgO, but all serum mineral concentrations were within normal physiological ranges. Although Mg-mica is relatively high in Fe (4%), using it as a supplemental Mg source appeared to have no negative impact on growth performance or serum mineral concentrations of calves grazing wheat pasture.     

Diabetes mellitus does not affect the neuromuscular blocking action of atracurium in dogs

Objective

To compare the duration of action of atracurium in diabetic and nondiabetic dogs.

Vitamin D status of wild Ricord's iguanas (Cyclura ricordii) and captive and wild rhinoceros iguanas (Cyclura cornuta cornuta) in the Dominican Republic.

Calcidiol (25-hydroxyvitamin D) values are reported for 22 wild Ricord's iguanas (Cyclura ricordii) and seven wild rhinoceros iguanas (Cyclura cornuta cornuta). Calcitriol (1,25-hydroxyvitamin D) values are reported for 12 wild Ricord's iguanas and seven wild rhinoceros iguanas. These animals were captured as part of a larger health assessment study being conducted on Ricord's iguanas in Isla Cabritos National Park, Dominican Republic. A total of 13 captive rhinoceros iguanas held outdoors at Parque Zoológico Nacional were also sampled for comparison. Mean concentrations of 25-hydroxyvitamin D were 554 nmol/L (222 ng/ml) with a range of 250-1,118 nmol/L (100-448 ng/ml) for wild Ricord's iguanas, 332 nmol/L (133 ng/ml) with a range of 260-369 nmol/L (104-148 ng/ml) for wild rhinoceros iguanas, and 317 nmol/L (127 ng/ml) with a range of 220-519 nmol/L (88-208 ng/ml) for captive rhinoceros iguanas. On the basis of these results, serum concentrations of at least 325 nmol/L (130 ng/ml) for 25-hydroxyvitamin D should be considered normal for healthy Ricord's and rhinoceros iguanas.     

Recovery from rocuronium-induced neuromuscular block was longer in the larynx than in the pelvic limb of anesthetized dogs

Objective

To determine if neuromuscular monitoring at the pelvic limb accurately reflects neuromuscular function in the larynx after administration of rocuronium in anesthetized dogs.

Pulsed inhaled nitric oxide improves arterial oxygenation in colic horses undergoing abdominal surgery

Objective

To evaluate the effect of pulsed inhaled nitric oxide (INO) on arterial oxygenation in horses during abdominal surgery.

Effects of Dietary Phosphorous and Trace Mineral Source on Immune Function,Mineral Status,and Performance of Stressed Steers

Two experiments were conducted to determine the effects of dietary P and trace mineral source on immune response, mineral status, and performance in steers stressed by weaning and disease exposure. In Experiment 1, 24 Angus and 24 Simmental weaned steers were used. Treatments consisted of 1) inorganic trace minerals, 2) organic trace minerals, 3) 0.15% supplemental P + inorganic trace minerals, and 4) 0.15% supplemental P + organic trace minerals. Copper, Mn, and Zn were added to provide 10, 25, and 25 mg/kg DM, respectively. The organic treatments supplied 50% of the supplemental Cu and Mn, and 66% of the supplemental Zn from metal proteinates, with the remainder supplied by inorganic sulfate forms. Inorganic treatments supplied all of the supplemental Zn, Cu, and Mn from sulfate forms. The basal diet was a corn silage-soybean meal-based diet. On d 2 following weaning, steers received an intranasal inoculation of infectious bovine rhinotracheitis virus (IBRV). Rectal temperatures in response to IBRV inoculation were similar across treatments. On d 9, steers were injected with 10 ml of a 25% pig red blood cell (PRBC) suspension. Total Ig titers against PRBC concentrations were higher (P<0.05) in steers receiving no supplemental P on d 7 postinjection. However, IgG and IgM titers were unaffected by treatment. Cell-mediated immune response (CMI) to phytohemagglutinin (PHA), plasma Cu and Zn concentrations, and 38-d performance were unaffected by treatment. In Experiment 2, 35 Angus steers were fed diets containing either inorganic or organic trace minerals. Performance and percent morbidity were unaffected by treatment. Plasma Cu was higher for steers fed organic trace minerals. Results indicate that increasing dietary P or replacing inorganic trace minerals with organic forms had little effect on immunity or performance of steers stressed by weaning.     

In vivo efficacy of tamarind (Tamarindus indica) fruit extract on experimental fluoride exposure in rats

The study was undertaken to determine the efficacy of hydro-methanolic (1:1) extract of tamarind (Tamarindus indica L.) fruit pulp in removing body fluoride burden. Thirty rats were divided into five groups. Keeping no fluoride group as the control, rats of no treatment, low dose, middle dose and high dose groups received sodium fluoride orally at the rate of 200 mg per kg body weight daily for 14 weeks. Rats of low dose, middle dose and high dose group simultaneously received tamarind fruit pulp extract at three doses, viz. 25 (low), 50 (medium) and 100 mg (high) per kg body weight orally, respectively. Fluoride concentration in blood, urine and long bone of experimental rats was monitored to assess the efficacy of the extract. Mean serum fluoride concentration in fluoride exposed rats was 0.145 ± 0.009 and 0.783 ± 0.042 μg/ml on days 0 and 98. In comparison, fluoride concentrations in tamarind treated rats were 0.179 ± 0.021 and 0.633 ± 0.015; 0.179 ± 0.021 and 0.502 ± 0.025 and 0.176 ± 0.021 and 0.498 ± 0.030 μg/ml in low, medium and high dose groups, respectively on day 0 and day 98 of the experiment. There was a significant (p ⩽ 0.01) increase in urinary fluoride excretion from day 28 onwards. The mean fluoride concentration in long bones of treated rats was significantly lower than the values recorded in fluoride exposed rats. These findings suggest that concomitant use of tamarind fruit pulp extract can reduce fluoride concentration in blood and bone and enhanced urinary excretion, indicating the ameliorative potential of fruits of tamarind in fluoride toxicity.     

The Immune Response and Performance of Calves Supplemented with Zinc from an Organic and an Inorganic Source

Two experiments were conducted to determine the effects of supplemental zinc (Zn) from an organic and an inorganic source on growth performance, serum Zn concentrations, and immune response of beef calves. Treatments consisted of: i) control (no supplemental Zn), ii) Zn sulfate, or iii) Zn–amino acid complex. Zinc sources were supplemented to provide 360 mg of Zn/d. Experiment 1 was a 28-d study using 84 steers (240 ± 1.5 kg) fed bermudagrass hay (21 mg Zn/kg DM) with 1.8 kg/d of the appropriate corn-based supplement. In Exp. 2, 75 heifers (176 ± 2.5 kg) were fed bermudagrass hay (38 mg Zn/kg DM) and the supplements for 140 d. In Exp. 1, ADG was greater (P<0.05) from d 15 to 28 in calves fed supplemental Zn-amino acid compared with those fed Zn sulfate, but ADG did not differ (P>0.10) among treatments for the entire 28-d study. In Exp. 2, there was no effect (P>0.10) on ADG as a result of Zn supplementation. In Exp. 2, Zn-supplemented heifers had a greater response (P=0.06) to phytohemagglutinin 24 h after an intradermal injection. In Exp. 2, calves supplemented with Zn–amino acid complex had a greater antibody response to a second vaccination for bovine respiratory syncytial virus than did control or Zn sulfate-supplemented calves (treatment by day interaction, P=0.06). There was not a consistent benefit of supplemental Zn on growth of calves, but there was a positive impact of supplemental Zn on some immune-response measurements.     

Pharmacokinetics of erythromycin after intravenous,intramuscular and oral administration to cats

The aim of this study was to characterise the pharmacokinetic properties of different formulations of erythromycin in cats. Erythromycin was administered as lactobionate (4 mg/kg intravenously (IV)), base (10 mg/kg, intramuscularly (IM)) and ethylsuccinate tablets or suspension (15 mg/kg orally (PO)). After IV administration, the major pharmacokinetic parameters were (mean ± SD): area under the curve (AUC)_(0–∞) 2.61 ± 1.52 μg h/mL; volume of distribution (V_z) 2.34 ± 1.76 L/kg; total body clearance (Cl_t) 2.10 ± 1.37 L/h kg; elimination half-life (t_½λ) 0.75 ± 0.09 h and mean residence time (MRT) 0.88 ± 0.13 h. After IM administration, the principal pharmacokinetic parameters were (mean ± DS): peak concentration (C_max), 3.54 ± 2.16 μg/mL; time of peak (T_max), 1.22 ± 0.67 h; t_½λ, 1.94 ± 0.21 h and MRT, 3.50 ± 0.82 h. The administration of erythromycin ethylsuccinate (tablets and suspension) did not result in measurable serum concentrations. After IM and IV administrations, erythromycin serum concentrations were above minimum inhibitory concentration (MIC)₉₀ = 0.5 μg/mL for 7 and 1.5 h, respectively. However, these results should be interpreted cautiously since tissue erythromycin concentrations have not been measured and can reach much higher concentrations than in blood, which may be associated with enhanced clinical efficacy.     

Effect of diet on serum 25-hydroxyvitamin D concentration in the short-beaked echidna (Tachyglossus aculeatus)

Background Elevated concentrations of 25‐hydroxyvitamin D (25(OH)‐D) were diagnosed in captive short‐beaked echidnas (Tachyglossus aculeatus) from three different zoological facilities within Australia. Results The mean serum 25(OH)‐D concentration in the wild echidnas was 24.7 nmol/L and was significantly higher in captive echidnas from all three facilities: Facility 1, mean 335.5 nmol/L (P < 0.001); Facility 2, mean 187.2 nmol/L (P = 0.003); Facility 3, mean 194 nmol/L (P = 0.005). Animals did not appear to have clinical manifestations of vitamin D toxicosis. The increased serum 25(OH)‐D concentration was attributed to excessive dietary intake and a reduction in the amount of vitamin D₃ in the diet of echidnas from Facility 1 resulted in a marked decrease in the serum 25(OH)‐D concentrations (mean 33 nmol/L). The reduction in serum 25(OH)‐D concentration was statistically significant (P = 0.002) and the resulting concentrations were similar to those of wild echidnas (P = 0.212). Conclusion It is not known what effect an elevated serum 25(OH)‐D concentration has on echidnas.     

Increased chymase-like activity in a dog with congenital pulmonic stenosis

ObjectivesThis study was intended to compare the tissue chymase-like activity and angiotensin converting enzyme (ACE) activity in the right and left ventricles of a dog with congenital pulmonic stenosis (PS), with normal dogs, and to discuss the potential clinical implications of these findings.Animals, materials and methodsStudy subjects included a one-year-old Beagle dog with spontaneous PS and six clinically normal Beagles. Chymase-like and ACE activities were determined in all hearts by high pressure liquid chromatography.ResultsIn the PS dog right ventricular (RV) chymase-like activity (49.79 nmol/min/g tissue) and left ventricular (LV) chymase-like activity (36.85 nmol/min/g tissue) were elevated vs normal Beagle dogs (mean ± standard deviation, RV: 20.17 ± 5.24 nmol/min/g, LV: 19.03 ± 3.27 nmol/min/g).ConclusionsActivation of the tissue RAA system was detected in a dog with congenital PS. This interesting finding should be pursued with further studies to validate this result, and to explore whether pharmacological blockade of chymase, or the angiotensin II receptor, represents a useful strategy to prevent myocardial remodeling in this condition.