Prospects for control of Pythium damping-off of lettuce with Trichoderma,Gliocladium and Enterobacter spp.

Summary Damping-off of lettuce (Lactuca sativa) caused by Pythium ultimum was studied in pots containing a non-sterile potting mix in the glasshouse. Fifty P. ultimum sporangia g^-1 compost reduced the plant stand to 15% and shoot dry weight to 18%, but this reduction was totally prevented by applying Trichoderma harzianum at 2×10⁵ viable propagules g^-1 potting mix. Gliocladium virens and Enterobacter cloacae also alleviated the damping-off. E. cloacae did not adversely affect the action of the fungal antagonists. The co-existence of the bacterial and fungal antagonists was revealed on the root surface and inner surface of the testa by scanning electron microscopy, indicating the compatibility of the biocontrol agents.     

Microbial community shifts in Pythium ultimum-inoculated suppressive substrates

In this study, the role of compost amendments for the biocontrol of Pythium ultimum was evaluated in bioassays with cucumber (Cucumis sativa L. variety “Chinesische Schlangen”). The addition of compost to the peat-based growing substrates resulted in a significant reduction of disease symptoms of cucumber plants in the presence of P. ultimum compared to pure substrate. Microbial community composition of compost-amended substrates and with different levels of P. ultimum inoculum (0, 5‰) was analyzed by polymerase-chain-reaction-based techniques. To detect and compare dominant bacterial and fungal representatives of suppressive substrate mixes with different pathogen inoculum, 16S and 18S rRNA clone libraries were established. Phylogenetic analysis of the 16S rRNA clones revealed Actinobacteria and α-Proteobacteria to be the prominent classes in the presence of P. ultimum, which are not part of the dominant microflora in the mixes without the pathogen. 18S rRNA sequences for the Pythium-inoculated compost supplemented samples were dominated by Chytridiomycota and Sordariomycetes, whereas in uncontaminated soil–compost mixes, a large part of the sequences were related to Homobasidiomycetes. Thus, it is assumed that the presence of P. ultimum induces distinct shifts in microbial communities favoring to groups known to comprise potential biocontrol agents.     

Improving quality of composted biowaste to enhance disease suppressiveness of compost-amended, peat-based potting mixes

Biowaste can be converted into compost by composting or by a combination of anaerobic digestion and composting. Currently, waste management systems are primarily focused on the increase of the turnover rate of waste streams whereas optimisation of product quality receives less attention. This results in low quality composts that can only be sold on bulk markets at low prices. A new market for quality compost could be potting mixes for horticultural container-grown crops to partially replace non-renewable peat and increase the disease suppressiveness of potting mixes. We report here on the effect of wetsieving biowaste prior to composting on compost quality and on disease suppressiveness against the plant pathogen Pythium ultimum of peat mixes amended with this compost. The increased organic matter and decreased salt content of the compost allow for significantly higher substitution rates of peat by compost. In this study up to 60% v/v compost peat replacement did not affect cucumber growth. However, disease suppressiveness of the potting mixes strongly increased from 31 to 94% when the compost amendment rate was increased from 20 to 60%. It was shown that general disease suppression for P. ultimum can only be effective when the basal respiration rate is sufficiently high to support microbial activity. In addition, organic matter of the compost should reach a sufficient stability level to turn from disease conducive to disease suppressive. Increasing the compost addition from 20 to 60% did not significantly affect plant yield, yield variation were due to differences in nutrient levels. It can be concluded that compost from wetsieved biowaste has high potential to replace peat in growing media for the professional market.     

Microbial enrichment to enhance the disease suppressive activity of compost

Compost amended soil has been found to be suppressive against plant diseases in various cropping systems. The level and reproducibility of disease suppressive properties of compost might be increased by the addition of antagonists. In the present study, the establishment and suppressive activity of two fungal antagonists of soil-borne diseases was evaluated after their inoculation in potting soil and in compost produced from different types of organic waste and at different maturation stages. The fungal antagonists Verticillium biguttatum, a mycoparasite of Rhizoctonia solani, and a non-pathogenic isolate of Fusarium oxysporum antagonistic to fusarium wilt, survived at high levels (10³–10⁵ CFU g^–1) after 3 months incubation at room temperature in green waste compost and in potting soil. Their populations faded out in the organic household waste compost, especially in the matured product. In bioassays with R. solani on sugar beet and potato, the disease suppressiveness of compost increased or was similar after enrichment with V. biguttatum. The largest effects, however, were present in potting soil, which was very conducive for the disease as well as the antagonist. Similar results were found in the bioassay with F. oxysporum in carnation where enrichment with the antagonistic F. oxysporum had a positive or neutral effect. We foresee great potential for the application of antagonists in agriculture and horticulture through enrichment of compost or potting soil with antagonists or other beneficial micro-organisms.     

Effects of fungivorous nematodes on corky root disease of tomato grown in compost-amended soil

Abstract

The effect of fungivorous nematodes, Aphelenchus avenae and Aphelenchoides spp., against corky root disease of tomato caused by Pyrenochaeta lycopersici was investigated. Three different greenhouse trials were conducted using soil naturally infested with P. lycopersici, alone or mixed with four different types of compost consisting of green manure, garden waste and horse manure (20% compost by volume). The fungivorous nematodes were propagated in cultures of the fungus Pochonia bulbillosa and inoculated (3 or 23 nematodes ml^?1 substrate) into the soil and soil-compost mixtures one day after transplanting of tomato seedlings. Greenhouse experiments were terminated after ten weeks and disease was measured from infected roots after harvesting. Aphelenchus avenae significantly reduced the disease severity when added to infested soil without compost in all experiments. Aphelenchoides spp. did not suppress the disease either in the presence or absence of compost. Among the composts tested, only a garden waste compost was found to be suppressive to the disease. Neither A. avenae nor Aphelenchoides spp. improved the suppressive effect of the compost.     

Nitrogen Availability Seven Years After a High-Rate Food Waste Compost Application

Long-term effects of compost application are expected, but rarely measured. A 7-yr growth trial was conducted to determine nitrogen availability following a one-time compost application. Six food waste composts were produced in a pilot-scale project using two composting methods (aerated static pile and aerated, turned windrow), and three bulking agents (yard trimmings, yard trimmings + mixed paper waste, and wood waste + sawdust). For the growth trial, composts were incorporated into the top 8 to 10 cm of a sandy loam soil at application rates of approximately 155 Mg ha^?1 (about 7 yd³ 1000 ft²). Tall fescue (Festuca arundinacea Schreb. ‘A.U. Triumph’) was seeded after compost incorporation, and was harvested 40 times over a 7-yr period. Grass yield and grass N uptake for the compost treatments was greater than that produced without compost at the same fertilizer N rate. The one-time compost application increased grass N uptake by a total of 294 to 527 kg ha^?1 during the 7-yr. field experiment. The greatest grass yield response to compost application occurred during the second and third years after compost application, when annual grass N uptake was increased by 93 to 114 kg ha^?1 yr^?1. Grass yield response to the one-time compost application continued at about the same level for Years 4 through 7, increasing grass N uptake by 42 to 62 kg ha^?1 yr^?1. Soil mineralizable N tests done at 3 and 6 yr. after application also demonstrated higher N availability with compost. The increase in grass N uptake accounted for 15 to 20% of compost N applied after 7-yr. for food waste composts produced with any of the bulking agents. After 7-yr, increased soil organic matter (total soil C and N) in the compost-amended soil accounted for approximately 18% of compost-C and 33% of compost-N applied. This study confirmed the long-term value of compost amendment for supplying slow-release N for crop growth.     

Comparison of Herbaceous Perennial Plant Growth in Seaweed Compost and Biosolids Compost

Liners of angelonia ‘Pink’ (Angelonia angustifolia Benth.), shooting star (Pseuderanthemum laxiflorum), coreopsis ‘Early Sunrise’ (Coreopsis grandiflorum Hogg ex Sweet.), and scutellaria ‘Purple Fountains’ (Scutellaria costaricana H. Wendl.) were transplanted into containers filled with, by volume, 1) 100% compost; 2) 60% compost, 25% vermiculite, 15% perlite; 3) 30% compost, 30% sphagnum peat, 25% vermiculite, 15% perlite; 4) 0% compost, 60% sphagnum peat, 25% vermiculite, 15% perlite. Two compost products were evaluated: SW, a 1:1 by volume mixture of partially composted seaweed and partially composted yard trimmings and SYT, a 1:1 by weight mixture of biosolids compost and yard trimmings compost. There was no difference in angelonia shoot dry mass among the different percentages of SW compost but angelonia plants grown in 0 and 30% SYT had greater shoot dry mass than plants grown in 60 or 100% SYT. Shoot dry mass of shooting star plants increased as the percentage of SYT compost increased from 0 to 30 % and then decreased while shoot dry mass of plants grown in SW compost decreased as the percentage of compost increased. There was no difference in coreopsis shoot dry mass or scutellaria shoot dry mass between the two compost products and there also was no difference in shoot dry mass among the different percentages of either compost product.     

Growth of Salt Sensitive Bedding Plants in Media Amended with Composted Urban Waste

Growth of Impatiens wallerana Hook.f (impatiens) and Antirrhinum majus L. (snapdragon) was evaluated in media containing 0, 30, 60, or 100 percent compost made from biosolids and yard trimmings. Shoot dry mass, size, and height of both impatiens and snapdragon linearly increased as the percentage of compost in the medium increased. Initial media soluble salt, nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) concentrations also linearly increased as the percentage of compost in the medium increased. However, final shoot N, P, K, Ca, and Mg concentrations in both impatiens and snapdragon plants were not different among the media examined. Results suggest that impatiens and snapdragons can be successfully grown in 100 percent compost made from biosolids and yard trimmings.     

Soils suppressive to Gaeumannomyces graminis var. tritici: Effects on other fungi

The effect of soils suppressive to Gaumannomyces graminis var. tritici (Ggt) on the severity of root and crown rots caused by Rhizoctonia solani, Gibberella zeae, Pythium irregulare, Cochliobolus sativus and Fusarium culmorum was tested in pot bioassays. An induced suppressive soil was obtained from the rhizosphere of wheat plants grown at 15°C for 28 days in fumigated soil inoculated with live inoculum (colonized oat grain) of Ggt.Root rot caused by R. solani was significantly less in soil amended with either induced or naturally suppressive soil. Disease caused by the other pathogens was also reduced by the induced suppressive soil, with the least reduction occurring with F. culmorum.Colonization of the surfaces of seminal roots of wheat plants by Gaeumannomyces graminis var. graminis (Ggg) and a Phialophora-like fungus (Plf 119) was also studied using the line-intercept method. In non-suppressive soil the maximum area of the primary seminal root colonized by Ggg was 7.4 per cent and by Plf 119 was 3.3 per cent. Colonization of roots by Ggg and Plf 119 was reduced substantially by the addition of induced suppressive soil.     

Effects of gibberellin A3 and 2,4-D on plant and root exudate lipids and susceptibility to Pythium myriotylum

Gibberellin A₃ or 2,4-dichlorophenoxyacetic acid were applied to the foliage of peanut plants under axenic culture and in the greenhouse. Leaves, roots and exudates from axenically grown plants were analyzed for total lipids, free sterols, free fatty acids and paraffinic hydrocarbons. The total lipid concentrations of leaves, roots and root exudates were not altered. The free sterol of roots, and the paraffinie hydrocarbon concentrations of both leaves and roots increased, but the free fatty acid of root exudates decreased. Plants treated in the greenhouse were rated for disease severity after soil infestation with Pythium myriotylum. Roots of treated plants exhibited less rot than roots of non-treated plants. We believe there are possibilites of altering disease susceptibility using foliar applications of growth regulators through their effects on root lipids and root-lipid exudation patterns.     

Applying Leaf Compost to Reduce Fertilizer Use In Tomato Production

For two consecutive years, one-inch (50 T/A) of leaf compost was applied to plots on a sandy terrace soil (Windsor, Connecticut) and a loamy upland soil (Mt. Carmel, Connecticut). These compost-amended plots were fertilized with 10-10-10 (N-P₂O_5-K₂O) at three rates: 0, 650 (half), 1300 (full) lb/A and cottonseed meal at a rate of 2166 lb/A. Tomato (Lycopersicon esculentum Miller) yield from compost-amended plots were compared to yield from unamended control plots fertilized with 1300 lb 10-10-10/A. In both years, at both sites, plots only amended with compost had yields equivalent to the fertilized control plots. In both years, the greatest yields at Mt. Carmel were from plots amended with compost and the full rate of inorganic fertilizer. In the second year, yields from compost-amended plots fertilized at half the rate were equivalent to compost-amended plots fertilized at full rate. The yields from the organic plots were similar to the control plots the first year and to plots amended with compost and half the rate of fertilizer the second year. At Windsor, the greatest yields for both years were from plots amended with compost and the full rate of fertilizer. The compost-amended plots fertilized with cottonseed meal produced the lowest yields, both years. Cumulative effects of compost on soils were measured by increases in pH and organic matter percentage at both sites. Tomato fruit in plots amended with compost and no fertilizer developed less blossom-end rot than fruit in all other treatments.     

Changes in tissue analysis caused by soil‐borne pathogenic fungl

Abstract

Several different organisms cause corn (Zea mays, L.) root rot. The extent of damage to the root system varies with the aggressiveness of different causal organisms or different strains of the same causal organism. The attack of the roots by disease organisms could cause changes in the tissue analysis that would lead to misdiagnosing the cause of the problem. Studies were conducted to ascertain changes in selected mineral concentrations in the corn tissue and in plant growth resulting from various degrees of root rot caused by root infections of several different soil borne pathogenic fungi. Thermally pasteurized soil was placed in pots and infested with various fungi isolated from root lesions on corn from field studies. Corn was grown in the infested soil for four weeks in the greenhouse. Plants were harvested, roots indexed for disease, above ground visual symptoms noted, dry weight taken, and tissue analyzed for Ca, Mg, P, and K. Results showed that aerial plant parts of infected plants may exhibit typical nutrient deficiency symptoms which are not necessarily reflected in the mineral analysis of the tissue. Elemental uptake and tissue concentration appeared not to be related to changes in mineral analysis regardless of the relative soil mobility of the nutrient. Related fungi did not cause similar changes in mineral tissue concentration for the same or different minerals. Dry matter yields were reduced about 20% for each unit increase in root disease index. The use of the root disease index appears to have potential as a diagnostic tool and should be evaluated for this purpose.     

Effects of Compost Source and Timing of Fertigation Initiation on Growth of Potted Poinsettia

Treatment combinations of four poinsettia (Euphorbia pulcherrima) cultivars, 20 substrates, and three fertigation treatments were evaluated in a completely randomized block design. Poinsettia cultivars included ‘V-14 Glory’, ‘Red Sails’, ‘Lilo Pink’, and ‘Annette Hegg Top White’. The compost substrates were compost:peat:perlite blended (by vol.) at 2:3:3, 1:1:1, and 2:1:1 ratios. Sunshine Mix 1 and Pro Gro 300S were used as controls. Composts tested were those prepared from crab offal, lime and polymer-dewatered biosolids, municipal solid waste (MSW), yard waste, and poultry litter. Fertigation treatments were initiated on the week of potting, delayed one or two week after potting and applied once per week. Controls produced premium quality plants. Composts prepared from poultry litter, yard waste, polymer-dewatered biosolids, crab offal, and MSW produced good quality plants. Plant width in 25 percent compost treatments were greater than those in 50 percent compost treatments. Total number of branches, plant height, canopy diameter, and plant grade on plants treated with fertilizer on the first week were only three percent greater than in treatments where fertilizer application was delayed one or two weeks.     

Long-term suppression of Pythium ultimum in arid soil using fresh and composted municipal wastes

 The effect of addition of municipal solid waste (MSW) at different degrees of stabilisation on the biological properties of an arid soil was studied 24 months after application. This included effects on the indigenous soil microflora and soil enzyme activities in the presence and absence of Pythium ultimum. The addition of organic waste (fresh or composted) reduced populations of culturable bacteria and fungi and disease symptoms caused by P. ultimum, and resulted in heavier plants with longer and more extensively branched roots. Addition of organic waste increased the population size of culturable bacteria and fungi, while enzymatic activity of the soil was higher in soil amended with organic matter than in non-amended soil. Populations of biological control agents, such as Trichoderma and fluorescent pseudomonads, were larger in soil amended with organic matter. The addition of urban waste could therefore be a suitable technique with which to restore soil quality by stimulating biological control against plant pathogens such as P. ultimum. Received: 3 May 1999     

Cumulative Effect of Leaf Compost on Yield And Size Distribution in Onions

For three years, a one inch layer of leaf compost was applied to plots on a sandy terrace soil in Windsor, Connecticut and fertilized with 1300 lb 10-10-10/A (N-P₂O₅-K₂O). Yields of four cultivars of onions (Allium cepa L.) (3 Spanish, 1 storage) from these plots were compared to yields from unamended control plots fertilized at the same rate. Year to year variability in yields was significantly lower in compost-amended plots. Yields from the unamended control plots (52% variation) fluctuated more than the compost-amended plots (3% variation) in response to variable rainfall from year to year. After three years of compost additions, yields from the compost-amended plots of the three Spanish onion cultivars were significantly greater than yields of these cultivars grown in unamended plots. The greater yields were due to both increased bulb weight and greater percent harvested. In two of three years, the compost-amended plots produced a greater percentage of colossal and jumbo sized onions in all cultivars. Repeated compost additions also reduced the incidence of soft rot disease, especially in susceptible cultivars in years with higher than average precipitation.     

Yard Trimmings as a Source of Nitrogen for Crop Production

Yard trimmings from sources rich in grass clippings have the potential to supply nutrients for crop production. Our objectives were to estimate N availability from yard trimmings and determine their effects on crop production, soil nutrients, and organic matter levels. We conducted a field experiment, comparing three consecutive years of yard trimmings applications (22, 44, or 66 Mg ha^?1 yr^?1 dry weight) with inorganic N (112 kg N ha^?1 yr^?1) and zero-N controls in a silage corn (Zea mays L.) - winter triticale (Triticosecale spp.) rotation. The yard trimmings were screened and ground, and allowed to heat for a short period. They were incorporated each spring before planting corn. We measured crop yield and N uptake, and estimated apparent N recovery (ANR). We measured soil inorganic N two weeks after yard trimmings application and after corn harvest. In a one-year on-farm demonstration, we compared three sources of yard trimmings applied at a single rate. Yard trimmings applied at 44 Mg ha^?1 dry weight provided sufficient available N to replace inorganic N. For silage corn grown with summer irrigation, estimated ANR in the crop was 7% in Year 1, 19% in Year 2, and 18% in Year 3 at the 44 Mg ha^?1 yard trimmings rate, compared with a mean ANR of 65% for the inorganic N treatment. Postharvest soil nitrate residual (0-to 120-cm depth) was similar for the 44 Mg ha^?1 treatment and inorganic N treatment. We observed variation in N availability with year and source of material. Yard trimmings also increased soil test K and organic matter.     

Quantification of Pythium populations in ginseng soils by culture dependent and real-time PCR methods

Ginseng (Panax quinquefolius L.) is widely cultivated in North America as a medicinal herb. However, yields are often reduced by various root pathogens, including Pythium species, which cause damping-off in young plants. In order to improve the prediction of disease risk, real-time PCR assays were developed and used in conjunction with dilution plating on selective media to quantify populations of Pythium irregulare Buisman and Pythium ultimum Trow directly from soil. The assays were tested on artificially infested soils and then used on a variety of naturally infested, ginseng-cultivated soils in south-western Ontario. Data on P. ultimum DNA concentrations were positively correlated with the number of P. ultimum viable propagules on selective media. However, in the case of P. irregulare, the presence of cryptic species resulted in incongruent relationships between the dilution plate and real-time PCR data. We therefore sequenced the ITS region of a large number of P. irregulare isolates in order to determine the proportion that would be detected by the real-time PCR assay. The ability to quantify pathogen populations directly from soils using real-time PCR (calibrated with data on inoculum potential) will improve disease risk assessment and lead to a reduction in pesticide application.     

Suppression of Strawberry Root Disease with Animal Manure Composts

Compost has been suggested as a nonchemical alternative to methyl bromide (MeBr) for control of root diseases. Its widespread availability and soil improvement properties are appealing to growers, but they need information on its use and effectiveness relative to the control of economically important root diseases in specific crops. Here, we report results from controlled-environment pot studies on the effects of manure composts on two major soilborne diseases of strawberry: red stele (RS) caused by Phythophthora fragariae, and black root rot (BRR), caused by a complex of fungi. Commercially available, on-farm-produced composts, based on either poultry/steer or dairy manure, were incorporated into two different, naturally infested soils at 5, 10, and 20% rates (v/v). Results show that significant control (36-79%) of RS in susceptible cultivar ‘Chandler’ occurred at all rates and with both types of compost in comparison with the unamended soil. Low rates (5%) of poultry/steer manure compost produced more disease control than equivalent or greater rates (10% and 20%) of dairy manure compost. However, the 20% rate had significantly greater soluble salts content than all other rates. At this 20% rate, root/shoot biomass accumulation and shoot health ratings were relatively low and equivalent to those from unamended soil, despite the improved content of soil organic matter, cation exchange capacity, and soil micronutrient content. The 5% amendment rate of poultry/steer manure compost provided more control than the 10% or 20% rates and more than the 5-20% rates of dairy manure compost. Soluble salts content in amended soils was significantly greater than in unamended soils, especially at 20% rates of poultry/steer compost. Leaf N from all treatments was deficient despite additional weekly supplemental fertilization. This deficiency coupled with increased soluble salt content at 20% poultry/steer compost amendments likely contributed to reduced root/shoot biomass accumulation and overall reduced plant vigor, thus increasing plant susceptibility to disease. In contrast to RS, BRR in all compost treatments was moderate in intensity and equivalent to the unamended soil, with slight but insignificant control at 5 and 10% rates of poultry/steer compost. Overall effects of the two manure compost amendments on BRR were inconclusive because no treatments, including the soil only, exhibited roots with extensive disease symptoms, despite cultural evidence of BRR pathogen colonization, especially by Rhizoctonia fragariae, during the course of this pot study. Effects of compost on appearance of BRR in strawberry should be pursued over a significantly longer time period than used here, possibly in field plots located on naturally infested soils subject to environmental stressors and seasonally realistic fertilization rates, because major BRR symptoms do not appear in the short-term even when fungal pathogens are present in the root.     

Growth of Impatiens ‘Accent Orange’ In Two Compost Products

Growth of Impatiens wallerana Hook. ‘Accent Orange’ was compared in two compost products made of biosolids and yard trimmings but differing in final particle size. The first product was screened to pass a 19 mm screen while the second product was screened to pass a 13 mm screen. Treatments consisted of 100 percent compost or substrates in which compost was combined with control substrate components at 60 percent, 30 percent, or zero percent compost. Shoot dry mass and size increased linearly as the percentage of each compost product in the growing substrate increased from zero to 100 percent. Final substrate electrical conductivity (EC), nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) concentrations also linearly increased as the percentage of each compost product increased in the substrate. Final plant size and shoot dry mass were significantly greater in the 13-mm product than in the 19-mm product. However, both the 13-mm and 19-mm products at 100 percent produced larger impatiens ‘Accent Orange’ plants compared with plants grown in zero percent compost.     

The influence of the root-knot nematode Meloidogyne incognita, the nematicide aldicarb and the nematophagous fungus Pochonia chlamydosporia on heterotrophic bacteria in soil and the rhizosphere

Plant‐pathogenic nematodes are a major cause of crop damage worldwide, the current chemical nematicides cause environmental damage, but alternatives such as biological control are less effective, so further understanding of the relationship between nematodes, nematicides, biological control agents and soil and rhizosphere microorganisms is needed. Microbial populations from roots of cabbage and tomato plants infested with the root‐knot nematode Meloidogyne incognita were compared with those from plants where the nematode was controlled by the nematicide aldicarb, or a nematophagous fungus with biological control potential, Pochonia chlamydosporia. The total numbers of culturable bacteria and fungi in rhizosphere soil were similar in all three treatments for both plants, around 100‐fold more than in control soil in which there were no plants. However, there were clear differences in the catabolic diversity, assessed by Biolog EcoPlate? carbon substrate utilization assays, between microbial populations from unplanted soil and the rhizosphere. In cabbage, a poor host for M. incognita, the rhizosphere population from P. chlamydosporia‐treated plants was distinct from the population from untreated and aldicarb‐treated plants. In tomato, a host susceptible to the nematode, the catabolic diversity of populations from aldicarb‐ and P. chlamydosporia‐treated plants was similar and differed from the untreated, nematode‐infested plants. The genetic diversity of the fast‐growing heterotrophic bacteria in the tomato rhizosphere, indicated by PCR fingerprinting with ERIC primers, was very different in the infested roots, whereas the profiles of isolates from both aldicarb‐ and P. chlamydosporia‐treated roots were similar. Evidently, nematodes have a greater impact on the rhizosphere population of a susceptible host, tomato, than a poor one, cabbage, and nematode‐infested roots are colonized by a different subpopulation of soil microbes from that on plants where infection is controlled, illustrating differences in root morphology and physiology.