Availability of Organic and Inorganic Phosphorus Fractions to Wheat in Toposequences of Calcareous Soils

Abstract

Information on the availability of different soil phosphorus (P) forms is useful for crop production. Phosphorus contents of 12 Iranian calcareous soils from upper‐, mid‐, and lower‐slope positions of two arid and two semiarid toposequences were fractionated to various organic and inorganic pools, and correlations of the P fractions with wheat responses were investigated. Among the inorganic P (IP) fractions, apatite type (Ca₁₀‐P) and dicalcium phosphate equivalents (Ca₂‐P) possessed the highest and the lowest amounts of P reserve in the soils, respectively. On average, about 20% of the total P was found in organic form (OP), of which 32% was labile (LOP), 51% was moderately labile (MLOP), and 17% was nonlabile (NLOP). The amounts of the soil P fractions were considerably influenced by the positions of the soils on the landscapes. The maximum contents of soil IP, Ca₂‐P, Fe‐P (iron‐bound P), and Ca₁₀‐P were observed in the lower‐slope positions. The amount of soil available [0.5 M sodium bicarbonate (NaHCO₃) extractable] P was significantly correlated with Ca₂P (r=0.895), Fe‐P (r=0.760), and Occl‐P (iron‐occluded P) (r=0.897). Direct correlation studies, however, showed that wheat shoot dry‐matter yield (DMY) was significantly affected by the amounts of Ca₂‐P, Fe‐P, OP, LOP, and MLOP fractions both at early (4 weeks) and late (10 weeks) stages of growth. All organic and inorganic P fractions, except Al‐P (aluminum‐bound P), Ca₈‐P (octacalcium phosphate equivalents), and NLOP, also showed significant relations to the amount and/or concentration of P in wheat tissues at 4 and 10 weeks after sowing. Among the measured soil properties, the amount of organic carbon was the most affecting factor on the size of the P fractions.     

Zinc Desorption of Calcareous Soils as Influenced by Applied Zinc and Phosphorus and Described by Eight Kinetic Models

The activity of zinc (Zn) ions in the soil solution bathing plant roots is controlled by the simultaneous equilibrium of several competing reactions, such as surface exchange, specific bonding, lattice penetration, precipitation reactions, and the processes leading to the desorption of surface and lattice-bound ions. Desorption of Zn in 15 calcareous soils from southern Iran, treated with 10 mg Zn kg^?1 soil as zinc sulfate (ZnSO₄·7H₂O) and 100 mg phosphorus (P) kg^?1 soil as calcium phosphate [Ca(H₂PO₄)₂H₂O] and then equilibrated and extracted with diethylentriaminepentaacetic acid (DTPA), was studied in this experiment. The results were fitted to zero-order, first-order, second-order, third-order, parabolic diffusion, two-constant rate, Elovich, and simple Elovich kinetic models. Two-constant rate, simple Elovich, and parabolic diffusion models were determined to best describe Zn-desorption kinetics. Zinc desorption increased as Zn was applied but decreased with applied P.     

Effect of Submergence on Iron Transformation and Phosphorus Availability in Calcareous Soils

A thermostatic incubation experiment was carried out to estimate the effects of flooding periods,stalk application and P addition of Fe transformation and P availability in calcareous soils.Submergence increased amorphous Fe,especially in the case of stalk application.The newly formed amorphous Fe with a great surface area played an important role in Psorption;and submergence also stimulated the dissolution of inorganic P,thus increasing the availability of soil P in calcareous soils.Meanwhile,a part of soluble P was absorbed and fixed again on the surface of newly formed amorphous Fe,thus resulting in a decrease of P availability.Soil rapidly available P increased after 150-day incubation.There existed significantly negative correlations between soil amorphous Fe content and soil Fe-P and rapidly available P contents.Submerged conditions promoted the transformation of inorganic P added toward Fe-P in calcareous soils,especially in the case of stalk application.     

Contrasting Effects of Four Plant Residues on Phosphorus Sorption-Desorption in Some Phosphorus Fertilized Calcareous Soils

Four types of plant residues (fruit waste, potato, sunflower, and wheat) with wide ranges of carbon to nitrogen (C/N) and carbon to phosphorus (C/P) ratios were added to the soil at the rate of 20 g kg^?1 (dry weight basis) and incubated for two months. In soils treated with plant residues, the P sorption ranged from 62.0% (potato) to 96.6% (wheat) and from 12.6% (fruit waste) to 50.6% (wheat) when 20 and 1500 mg P kg^?1 were added to the soils, respectively. In general, incorporation of plant residues decreased maximum P sorption capacity but increased bonding energy. The maximum P sorption capacity was reduced from 586 mg kg^?1to 500, 542, and 548 by wheat, fruit, and potato residues, respectively, but increased to 665 mg kg^?1 by sunflower residue. At higher P addition, the highest percentage of desorbed P was observed in soils treated with wheat residue (49.9%); followed by fruit waste (46.5%), potato (43.5%), sunflower (38.8%) and control soils (37.0%). It indicated that the P content of the organic residues had an important role in the sorption and desorption of P in calcareous soils. Among organic residues, sunflower residue showed high sorption and low desorption of P in soils, indicating a higher potential of this organic residue for P retention and reducing surface and groundwater contamination in calcareous soils.     

低分子量有机酸对石灰性土壤有机磷组成及有效性的影响

为探索提高土壤磷素有效性的途径,采用室内培养的方法,研究不同有机酸对土壤速效磷含量及有机磷组分的影响。结果表明,添加有机酸后土壤速效磷含量发生显著变化,其中草酸处理下土壤速效磷含量显著高于其他处理,而柠檬酸和苹果酸对土壤速效磷含量具有抑制作用,其活化量为负值;随着培养时间的延长,速效磷含量缓慢降低。速效磷含量随着草酸浓度的升高而升高,随着苹果酸、柠檬酸浓度的升高而降低;有机酸处理后,土壤活性、中活性、中稳性有机磷升高,高稳性有机磷降低,这说明有机酸能促进土壤有机磷由有效性低的形态逐步向有效性高的形态转化,其中草酸的作用效果总体上较柠檬酸和苹果酸强。     

Role of Phenolics and Organic Acids in Phosphorus Mobilization in Calcareous and Acidic Soils

Abstract

Phenolic acids (caffeic, CAF; protocatechuic, PCA; p-coumaric, COU; and vanillic, VAN), catechol (CAT), poly-galacturonic acid (PGA), and citric acid (CIT) were compared for their effectiveness in phosphorus (P) mobilization in three soils differing in chemical properties. The addition of organic ligands at 100 μmol g^{? 1} soil increased the concentrations of resin P (P_r), water-extractable P (P_w), and bicarbonate-extractable inorganic P (P_bi), thus improving the phosphorus availability. The magnitude of P mobilization in the calcareous soil can be expressed in the following order: CAF > CAT > PCA = CIT > VAN > COU > PGA, which was consistent with the number of phenolic hydroxyl groups they contained and the position of carboxyl on the benzoic ring. In the two acid soils tested, the order of P mobilization was CIT > CAT > PCA > CAF after 24 h incubation, and CIT > PCA > CAF > CAT after a 14 d incubation. The mobilized P originated partly from the organic P fractions, which could be extracted by 0.5 M NaHCO₃. In addition, P_r decreased and P_w increased during incubation. The exceptions were that the CAF treatment increased P_r and the CIT treatment did not affect P_w. Calcium extraction from the soils after a 1 d or 14 d incubation could not fully account for the P mobilization. The results suggest that the inorganic P dissolution by the organic ligands was not the only mechanism of P mobilization in the calcareous soil, while in acid soils the chelation of metal cations by organic ligands is likely an important factor in P mobilization.     

氨基酸对三种石灰性土壤磷素有效性的影响及其作用差异机制

磷是农作物生长发育的必需元素,为了保证我国粮食安全,提高农作物的产量,提高磷肥有效性十分重要。本试验选取三种石灰性土壤（郑州潮土、周口褐土和昌图风沙土）为研究对象,通过90天的室内埋土试验,在三种土壤中研究氨基酸与磷酸一铵配施对提高土壤中磷肥有效性的影响。研究结果表明:（1）氨基酸在三种石灰性土壤中均能够提高磷酸一铵的有效性,郑州潮土、周口褐土和昌图风沙土的磷肥有效性与单施磷酸一铵相比分别提高了21.16%、10.87%和4.06%;（2）氨基酸的加入降低了土壤中Ca₂-P向Ca₈-P或其他难溶形态磷的转化,通过对三种土壤进行相关性和通径分析的得出在郑州潮土、周口褐土和昌图风沙土中主要决策因子是Ca₂-P,决策系数分别到达了0.836、0.946和0.712（P < 0.05）,郑州潮土、周口褐土主要限制因子是Ca₈-P,决策系数分别为?0.066、?0.401（P < 0.05）,昌图风沙土主要限制因子是Ca₈-P和Fe-P,决策系数分别为?0.080和?0.105（P < 0.05）;（3）氨基酸的加入能够降低三种石灰性土壤的pH和CaCO₃含量,有利于提高磷肥有效性;（4）通过对三种土壤的有效磷含量与土壤理化性质进行冗余分析,得出有机质（SOM）和碳酸钙 （CaCO₃）是影响磷肥在三种土壤中固定速率差异的主要原因,SOM和CaCO₃分别解释了有效磷含量全部变异的36.5%和25.6% （P < 0.05）。氨基酸在三种石灰性土壤中均能够提高磷肥有效性,主要途径是降低土壤的pH和CaCO₃含量,抑制Ca₂-P的快速转化。在三种石灰性土壤中,氨基酸作用有差异的主要原因是三种土壤中SOM和CaCO₃含量的差异,提高SOM,降低土壤中CaCO₃能够降低土壤对磷的固定,提高磷肥有效性。     

Phosphorus Availability in Low-P and Acidic Soils as Affected by Liming and P Addition

Acidic soils typically suffer from high phosphorus (P) retention, a problem that can be dealt with using greater P fertilization, soil liming, or both. The aim of this work was to examine which of these practices bears the more beneficial result for Lolium perenne L. growth. In a pot experiment, five acidic soils were treated as follows: L0P0 (unamended control), L1P0 (liming only), L0P1 (P addition only), and L1P1 (both liming and P addition). We found that P amendment alone was sufficient to increase plant P levels when the initial soil P concentrations were low. Liming without P addition increased plant P satisfactorily only in the high-P soil. We conclude that P addition alone is a better practice than liming alone for improved plant growth conditions in acidic, low-P soils, unless there is relatively high P content in soil, in which case liming alone may be sufficient to increase P availability.     

Phosphorus Fertilizer use in Pineapple Cultivation with in situ Residues Burning on Organic Soils

Abstract

In Malaysia, pineapples are grown on peat soils, but most phosphorus (P) fertilizer recommendations are made without due quantification of P uptake; the distribution of P in roots, stem, leaves, peduncle, fruit, and crown; or loss through leaching even though P retention in peat soils is low. This study was conducted to determine applied P‐use efficiency under a conventionally recommended fertilization regime in pineapple cultivation with in situ residues burning before replanting. Results showed that most of the P uptake in pineapple can be found in the fruit, stem, leaves, and crown, but the general trend of P distribution was in the order of fruits>leaves>stem>crown>peduncle>roots. Phosphorus recovery in pineapple cultivation was about 40%, and this low recovery was attributed to leaching. Hence, fertilizer recommendations need to take into consideration P loss through leaching. This will help to increase P‐use efficiency because it is not possible to build up P content of peat soils. As a result, the need to assess the possibility of side‐dress applications of phosphatic fertilizers on peat soil is necessary.     

有机酸对石灰性潮土有机磷组分的影响

采用小麦盆栽试验的方法,研究了有机酸对石灰性潮土有机 P 组分含量及土壤 P 有效性的影响.结果表明:石灰性潮土的 4 种有机 P 组分中,以中度活性有机 P 为主,其次为高稳性有机 P 和中稳性有机 P ,活性有机P的含量最低;施用磷酸二氢钾和氟磷灰石后,土壤有机P总量和有机P各组分发生了变化;土壤经有机酸处理后,有机P总量增加,有机P总量的增加主要是中度活性有机P和活性有机P的增加.而中稳性有机P和高稳性有机P主要表现为下降,说明有机酸能够促进土壤有机P由有效性较低的形态逐步向有效性高的形态转化,从而进一步矿化为无机P或者被植物根系直接吸收,其中草酸的作用效果总体上较腐殖酸强;在P素供应不足的情况下,有机酸(草酸和腐殖酸)能够提高石灰性潮土中P的有效性,促进植物的正常生长.     

Evaluation of Chemical Indices of Soil Pb Availability in Calcareous Soils

The objective of this study was to compare six soil tests (1 M KNO₃, 1 M NH₄OAC, 0.005 M DTPA, 0.1 M EDTA, 1 M HNO₃ and 0.025 M Ca DTPA B₄O₇) as extractants for soil Pb and as predictors of plant available Pb for wheat (Triticum aestivum L.) in the greenhouse. The soils recieved 0, 200 and 400 mg Pb kg^?1 as Pb(NO₃)₂ and are referred to as Pb₀, Pb₁ and Pb₂ treatments respectively. Of the six soil extractants, 1 M HNO₃ was the most effective extractant for Pb from Pb₀ treatment whereas 0.1 M EDTA and 0.025 M Ca DTPA-B₄O₇ were the best and equally effective in their ability to extract Pb from Pb₁ and Pb₂ treatments. Regression analysis was used to develop two variable models for predicting Pb uptake by wheat as a function of extractable Pb and selected soil properties. The 0.025 M Ca DTPA-B₄O₇ extractant was the best in predicting uptake by wheat in Pb₀ (r = 0.791^*** significant at p = 0.001) and Pb₁ (r = 0.726^***) and Pb₂ (r = 0.942^***) treatments.     

Inorganic Phosphorus Fractionation of Highly Calcareous Soils of Iran

Abstract

Highly calcareous soils are abundant in Iran. The calcium carbonate equivalent (CCE) of these soils reach up to 650 g kg^?1. Although phosphorus (P) fertilizer is being widely used in these soils, little information, if any, is available about P status in such soils. The objectives of this study were to 1) determine inorganic P forms in 18 surface soils of southern Iran, 2) study P readsorption during different stages of fractionation schemes, 3) assess the ability of NaOH to extract aluminum (Al)‐P, and 4) evaluate the relationships between P availability indices and inorganic P forms. Eighteen soil samples with a wide range of physicochemical properties were selected for this study. Inorganic P forms was determined by sequential extraction with NaHCO₃, NH₄OAc, NH₄F, NaOH, citrate dithionite (CD), and H₂SO₄, which are referred to as Ca₂‐P, Ca₈‐P, Al‐P, Fe‐P, occluded P (O‐P), and Ca₁₀‐P. Phosphorus readsorption in different stages was determined by 1 M MgCl₂. Furthermore, a fractionation scheme without an NH₄F step was used to evaluate the ability of NaOH to extract Al‐P. NaHCO₃ (Olsen‐P) and MgCl₂‐extractable P (Exch‐P) were regarded as P-availability indices. The abundance of different P forms was in the order Ca₂‐P<Fe‐P<Al‐P<O‐P<Ca₈‐P<Ca₁₀‐P. Ca₂‐P was highly correlated with Olsen‐P and Exch‐P. Ca₂‐P, Olsen‐P, and Exch‐P showed a relationship with CCE, citrate–bicarbonate–dithionite extractable Fe (Fe_d), and Al (Al_d). Phosphorus readsorption appeared to be important only in the Ca₈‐P step, and the content of readsorbed P was related to Ca₈‐P, CCE, and clay content of the soils. In the present study, Al‐P and Fe‐P accounted for 10 and 5% of the sum of the inorganic P fractions, respectively, and Fe‐P showed a strong relationship with Fe_o, whereas Al‐P showed a significant relationship with oxalate‐extractable Al (Al_o) and Al_d. It was found that one extraction with NaOH is not a good indicator for Fe‐ and Al‐P, and the ability of NaOH to extract Al‐P was reduced with increase in Al‐P content.     

Phosphorus Use Efficiency of Wheat Genotypes Grown in Calcareous Soils

ABSTRACT

Phosphorus (P) deficiency and low P-use efficiency are induced by soil properties, especially in calcareous soils, which are dominant in semi-arid regions of the world such as the Mediterranean region. Alternative approaches to P fertilization involve exploiting plant genetics in order to achieve more efficient use of P by the growing crop. Accordingly, in a greenhouse pot experiment, we evaluated P-efficiency in wheat genotypes grown in typical calcareous soils in southern Turkey. Ten common local genotypes were grown in six soil series for eight weeks using five P application rates (0, 25, 50, 100, and 200 mg P kg^?1). Dry matter (DM) yield and P content were significantly increased by increasing P rates, with significant differences between soils. Some genotypes performed better under P stress because of better P utilization efficiency. Shoot DM was the most sensitive indicator of genetic variability under P-deficient conditions. Genotypes classified as efficient-responsive (Adana-99, 1014, Golia, Balatilla) had above average DM yield when P was not added, and responded well to P applications; efficient-non-responsive genotypes (Firat-93, Seri-82, Genc-99, Panda) had below average DM yield, but responded to P applications; inefficient-non-responsive genotypes (Fuat Bey and Ceyhan-99) had below average DM yield; and no genotypes were in the inefficient responsive category. Such P response categorization is needed for better breeding programs for nutrient-use efficiency. The study may aid in selecting P-efficient genotypes in low-P soils, especially where little P is used. The use of P-efficient genotypes should be seen as complement to, rather than a substitute for, chemical P fertilization depending on local conditions.     

施加粪肥对潮土有机磷形态转化的影响

施加粪肥是提高土壤肥力的重要措施,为了解粪肥磷在潮土中的化学行为,通过室内培养试验,采用NaOH-EDTA 浸提和 31P 核磁共振技术分析比较了鸡粪、牛粪及施肥后土壤中的磷形态及含量,并研究了施肥对潮土有效磷的影响。结果表明,粪肥磷主要以无机态形式存在,2 种粪肥的有机磷形态及含量有明显不同,肌醇六磷酸在鸡粪中的含量明显高于牛粪。粪肥施加到潮土后丰富了土壤有机磷的形态。随时间延长,潮土中各形态磷发生相互转化,以肌醇六磷酸为主的正磷酸单酯含量明显降低,核酸等正磷酸双酯显著升高。鸡粪处理的土壤有效磷含量逐渐升高,牛粪处理则表现出相反的趋势。施加粪肥后,土壤有效磷呈现不同的变化规律可能是无机磷在土壤中固定或沉淀,有机磷矿化和无机磷被微生物固持这三方面综合作用的结果。     

Phosphorus Availability in Soils Amended with Different Phosphate Fertilizers

Abstract

Accurate measurement and characterization of phosphate rock dissolution are important for a better understanding of phosphorus (P) availability in soils. An incubation study was carried out on two New Zealand topsoils (0–15 cm; high P buffering capacity Craigieburn and low P buffering capacity Templeton) amended with North Carolina phosphate rock (NCPR) and water‐soluble phosphate (WSP) at 218 mg P kg^?1 (equivalent to 60 kg P ha^?1). Isotopic exchange kinetics was carried out after 12 h and 28 days of incubation to characterize P availability. This study showed that sensitivity of capacity factors (r₁/R, n) to explain changes in E_1min values was affected by the P buffering capacity of the soils. The recovery of applied P in the E pool (Rec_inE%) with extended incubation time was similar from the NCPR and WSP treatments (3.1–3.3%) in the Craigieburn soil compared with the Templeton soil in which Rec_inE% values were greater in WSP (9%) than NCPR (1.3%) treatment. The higher values of P derived from the applied P fertilizers in the E pool (Pdff_inE%>80%) suggested that the NCPR application in both soils would be efficient for increasing P availability to plants.     

Genotypic Responses of Corn to Phosphorus Fertilizer Rates in Calcareous Soils

In the Mediterranean region, much emphasis is placed on the role of fertilizers in enhancing crop production to achieve food security. Given the complex nature of phosphorus (P) reactions in soils, considerable research has dealt with fertilizer aspects related to efficient P use, but comparatively less emphasis has been given to plant variation with respect to P efficiency. In this study, selection and adaptation of P‐efficient corn genotypes was seen as one possible approach to enhancing P efficiency. Thus, a greenhouse experiment with 10 corn genotypes (traditional to modern), five P application rates (0–200 mg kg^?1), and four field trials with three genotypes for 2 years were carried out on various calcareous soils (Vertic Torrifluvent, Vertic Calciorthid, Entic Chromoxerert, and Typic Xerofluvent). Measurements were made of root characteristics. Treatments in the field trials were five P application rates as main plots (0–68 P ha^?1) and three corn genotypes as subplots. Genotypes were selected for the field trials from the greenhouse experiment as “efficient‐responsive,” “efficient‐nonresponsive,” and “inefficient‐responsive.” Dry‐matter (DM) yield and plant P uptake by plants increased with P application rates in the greenhouse experiment. Root length and mass were considerably increased by increasing P levels. Genotypes were classified for P efficiency. The studies indicated that because corn genotypes respond to P‐fertilizer application differently, this trait could be utilized to exploit native and applied P more efficiently, especially at low levels of available P and when P‐ fertilizer use is limited. This differential response derives from morphological, physiological, and genetic variability among the genotypes. Although genotypic efficiency is important for fertilizer management, the contribution of the efficiency is not a substitute for fertilizers, especially if high yields are required. Nevertheless, breeding for P‐use efficiency should be a component of any program to improve crop yield potential.     

Influence of Organic Manure on Organic Phosphorus Fraction in Soils

The transformation of organic P(Po) from organic manures in two types of soils (ultisol and entisol) and the influences of external addition of organic substance or inorganic P(Pi) on Po under the condition of the 60% maximum water capacity were investigated.The results obtained from Po fractionation experiments indicated that all the Po fractions except for the highly resistant Po fraction decreased during incubation.Application of pig feces and cow feces could largely increase each fraction of Po in the soils.Immediately after application of organic manure into the soils a large part of labile and moderately labile Po from organic manure was transferred into moderately resistant Po,which might be due to the fact that Ca-or Mg-inositol P was precipitated into Fe-inositol P.However,the availability of Po from organic manure in the soils would increase again after incubation because of the transformation of moderately labile and resistant Po fractions into labile Po fractions.Addition of cellulose or Pi into the soils showed a good effect on increasing all the Po fractions except for the highly resistant Po,and this effect was much more pronounced when cellulose was applied in combination with Pi.Therefore,in view of the effect of organic manure on improving P nutrition to plant,attention should be paid to both the Po and the organic substances from organic manure,It is suggested that application of Pi fertilizer combined with organic manure may be referred to as an effective means of protecting Pi from chemical fixation in soil.     

Transformations and Availability of Iron to Wheat as Influenced by Phosphorus and Manganese Fertilization in a Typic Haplustept Soil

An investigation was conducted using Typic Haplustept, sandy loam soil, to investigate the interactive effects of phosphorus (P) and manganese (Mn) fertilization on native iron (Fe) pools in soil and their availability to wheat (cv. PBW-343) crop. Phosphorus fertilization moved Fe from residual mineral fraction of Fe to manganese oxides (MnOX), organic matter (OM), amorphous (AMPOX), and crystalline (CRYOX) Fe and Al oxide fractions. However, Mn application decreased specifically adsorbed (SAD)–Fe and CRYOX–Fe but increased OM–Fe and mineral fraction of Fe. Available Fe in soil decreased as Olsen P and P:Mn ratio increased in the soil. Higher Olsen P (>60 mg P kg^?1soil) reduced mean Fe uptake by shoot. P content and P:Mn ratio in soil as well as in root and shoot were inversely related to Fe concentration in both the plant parts. The role of soil Fe associated with oxides and organic matter was found most notable in Fe nutrition of wheat.     

Soil Properties as Influenced by Incorporation of Crop Residues

Chemical transformation,nutrient release and changes in the energy content of decomposing rape,sunflower and soybean residues confined in buried fiberglass bage were assessed in a laboratory study during a 340-day incubation period.The organic C decreased by about 70% while total N,after initial decay,remained almost constant for each type of residue.The NH4^ -N was progressively oxidized and,consequently,the NO3^-N increased.The C/N ratio narrowed with loss of C.Hemicellulose,cellulose and lignin decreased with different trends for each crop residue.The energy content,was reduced to about 25% in rape,30% in sunflower and 20% in soybean residues.Total P,K,Ca and Mg contents decreased for each type of residue The results suggest that the three residues could imporve the nutrient pool of the soil.