共查询到20条相似文献,搜索用时 31 毫秒
1.
Comparative Study of CaMsrB2 Gene Containing Drought‐Tolerant Transgenic Rice (Oryza sativa L.) and Non‐Transgenic Counterpart
下载免费PDF全文
![点击此处可从《Journal of Agronomy and Crop Science》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S. K. Dhungana B.‐R. Kim J.‐H. Son H.‐R. Kim D.‐H. Shin 《Journal of Agronomy and Crop Science》2015,201(1):10-16
Concerns regarding the safety of transgenic foods have been raised because of possibility of undesirable effects development during genetic engineering. Analysis of phenotypic traits can increase the likelihoods of identifying those unintended effects in dietary composition of the GM crops. Objective of this study was to compare the transgenic lines with their non‐transgenic counterpart. Different vegetative and reproductive traits as well as antioxidant properties were considered to evaluate the transgenic (HV8 and HV23) lines containing CaMsrB2 gene and their non‐transgenic (Ilmi) parent line. Grain size and weight, seed germination, root length, root and shoot dry weight, length and width of flag leaf, plant height, and ligule, stamen and carpel length were not significantly different. Onset and completion of heading in each line occurred almost during the same period. The antioxidant properties in terms of DPPH (1,1‐diphenyl‐2‐picrylhydrazyl) radical scavenging activity and polyphenol content were not statistically different under same treatment condition. The results suggested that the transgenic rice lines containing CaMsrB2 gene were equivalent to their non‐transgenic counterpart without any visible unintended effects. 相似文献
2.
3.
É. Darkó J. Fodor S. Dulai H. Ambrus A. Szenzenstein Z. Király B. Barnabás 《Journal of Agronomy and Crop Science》2011,197(6):454-465
To improve the abiotic stress tolerance of maize (Zea mays L.), doubled haploid (DH) plants were produced by in vitro selection of microspores exposed to tert‐butyl hydroperoxide (t‐BuOOH) as a powerful prooxidant This study investigated the tolerance of the progenies of t‐BuOOH‐selected DH lines to oxidative stress, cold and drought in controlled environment pot experiments by analyses of photosynthetic electron transport and CO2 assimilation processes, chlorophyll bleaching and lipid peroxidation of leaves. Our results demonstrated that the t‐BuOOH‐selected DH plants exhibited enhanced tolerance not only to oxidative stress‐induced by t‐BuOOH but also to cold and drought stresses. In addition, they showed elevated activities of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase and glutathione S‐transferase when compared with the DH lines derived from microspores that were not exposed to t‐BuOOH and to the original hybrid plants. The results suggest that the simultaneous up‐regulation of several antioxidant enzymes may contribute to the oxidative and cold stress tolerance of the t‐BuOOH‐selected DH lines, and that the in vitro microspore selection represents a potential way to improve abiotic stress tolerance in maize. 相似文献
4.
The effect of 0, 0.05 or 0.1 mm abscisic acid treatment on chilling tolerance and salicylic acid‐related responses was investigated in young maize seedlings (Zea mays L., hybrid Norma). Although the pre‐treatment of maize seedlings with abscisic acid slightly decreased the chlorophyll content, it also reduced the level of chilling injury caused by 6 days of cold treatment at 5 °C. Under normal growth conditions, increased levels of bound salicylic acid and of bound ortho‐hydroxycinnamic acid were observed in the leaves during abscisic acid treatment. In the roots, abscisic acid did not affect the free and bound salicylic acid levels, but increased the amount of free and bound ortho‐hydroxycinnamic acid. The activity of glutathione‐S‐transferase increased on the 3rd day of abscisic acid treatment, whereas it did not change when followed by cold stress, compared with the control leaves. In the roots, the activities of glutathione reductase, glutathione‐S‐transferase and ascorbate peroxidase increased during the abscisic acid treatment, and those of glutathione‐S‐transferase and ascorbate peroxidase were also stimulated when abscisic acid pre‐treatment was followed by cold stress, compared with the control roots. Our results suggest that an overlap may exist between the abscisic acid‐induced cold acclimation and the salicylic acid‐related stress response. 相似文献
5.
Transgenic soybeans expressing betaine aldehyde dehydrogenase from Atriplex canescens show increased drought tolerance
下载免费PDF全文
![点击此处可从《Plant Breeding》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Betaine aldehyde dehydrogenase (BADH) catalyses the oxidation of betaine aldehyde to glycine betaine. To test whether BADH can increase drought tolerance in soybean (Glycine max), BADH from the drought‐tolerant plant Atriplex canescens (AcBADH) was introduced into the soybean cultivar ‘Jinong 17’ by Agrobacterium‐mediated cotyledon transformation. Eight independent AcBADH transgenic lines were subjected to drought stress. As expected, AcBADH was expressed in transgenic soybean leaves and not in the control. In transgenic plants, AcBADH expression increased following drought treatment. Under osmotic stress, the germination index was 6%–17% higher in the transgenic lines than in the control. Using a randomized block design, we measured drought‐related physiological indices and yield traits. The proline content in AcBADH transgenic soybeans increased by 12.5%–16.6%, peroxidase activity increased by 1%–7%, dry weight of plant increased by 15%–20% and malondialdehyde contents decreased by 1.5%–13%, compared to the control. Under drought conditions, two of the eight transgenic soybean lines had higher yields than the control, with increases of 7.59%–8.84%. Therefore, transgenic expression of AcBADH may provide a promising strategy to engineer drought tolerance without adverse consequences. 相似文献
6.
Deployment of cold tolerance loci from Oryza sativa ssp. Japonica cv. ‘Nipponbare’ in a high‐yielding Indica rice cultivar ‘93‐11’
下载免费PDF全文
![点击此处可从《Plant Breeding》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Cold tolerance is a complex trait, and QTL pyramiding is required for rice breeding. In this study, a total of seven QTLs for cold tolerance in the Japonica rice variety ‘Nipponbare’ were identified in an F2:3 population. A stably inherited major QTL, called qCTS11, was detected in the region adjacent to the centromere of chromosome 11. In a near‐isogenic line population, the QTL was further dissected into two linked loci, qCTS11.1 and qCTS11.2. Both of the homozygous alleles of qCTS11.1 and qCTS11.2 from ‘Nipponbare’ showed major positive effects on cold tolerance. Through pyramiding the linked QTLs in the cold‐sensitive Indica rice cultivar ‘93‐11’, we have developed a new elite, high‐yielding Indica variety with cold tolerance. 相似文献
7.
Galactinol synthase (GolS) is considered to be a key regulator of the biosynthesis of Raffinose family oligosaccharides (RFOs). Accumulation of RFOs has been reported to play a role in protection against abiotic stresses. We identified two cDNAs encoding galactinol synthase from wheat (Triticum aestivum L.), which we designated as TaGolS1 and TaGolS2. Expression of the two TaGolS genes was induced by cold stress but not by drought, heat stress or ABA treatment in wheat. We generated transgenic lines of rice (Oryza sativa L.) constitutively overexpressing TaGolS1 or TaGolS2. These transgenic plants accumulated significantly higher levels of galactinol and raffinose than did wild-type plants and exhibited enhanced cold-stress tolerance. The results demonstrate the involvement of galactinol and raffinose in the development of chilling stress in rice and indicate that the genetic modification of the biosynthesis of RFOs by transformation with GolS genes could be an effective method for enhancing chilling-stress tolerance in rice. 相似文献
8.
Changqing Xuan Yaping Liu Chengjie Liu Lewis Bongani‐Tafadzwa Lusuwi Xiaojin Fu Xifeng Chen Bojun Ma 《Plant Breeding》2019,138(3):290-298
Commercial application of nuclear genetic male sterility has been improved recently by a novel technique, Seed Production Technology (SPT), which incorporates transgenic maintainer lines capable of propagating non‐transgenic nuclear male‐sterile lines for use as female parents in hybrid production. Here, we identified a rice nuclear male‐sterile mutant, Oswbc11, with abnormal pollen development and lipid transport. We finely mapped the Oswbc11 gene into a 12.5‐kb region on chromosome 10 and found one candidate gene, which had a base substitution (C to T) resulting in a premature stop codon and was functionally confirmed by CRISPR/Cas9 technology. Gene OsWBC11 encodes a sub‐family member of adenosine triphosphate‐binding cassette transporter, which participates in the active transport of a wide range of molecules across membranes. Moreover, the agronomic traits of Oswbc11 mutants showed no significant differences compared to the wild‐type control except for the seed setting rate. These results indicated that Oswbc11 gene could be used in rice hybrid breeding as a recessive nuclear male‐sterile gene combined with CRISPR/Cas9 and the SPT technology and applied in different rice varieties. 相似文献
9.
Identification of an ice recrystallisation inhibition gene family in winter‐hardy wheat and its evolutionary relationship to other members of the Triticeae
下载免费PDF全文
![点击此处可从《Journal of Agronomy and Crop Science》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S. X. Guan M. Zhong H. Ma S. Wang Z. F. Guo 《Journal of Agronomy and Crop Science》2018,204(4):400-413
Ice recrystallisation inhibition (IRI) proteins are important for plants that exist in cold environments. Six novel genes encoding IRI proteins were isolated from winter‐hardy wheat (Tricticum aestivum) cultivar (Mironovskaya808). Conserved domain analysis confirmed that all the predicted proteins contained the basic characteristics of IRI proteins, including leucine‐rich repeats (LRR) and IRI‐domains (NxVx1‐2G). In addition, these proteins possessed an N‐terminal signal peptide and some conserved cysteines. Despite the high conservation of the amino acid sequences, the characteristic regions still exist among these IRI‐domains of IRI sequences. All IRI sequences in this study are highly homologous with phytosulfokine receptors (PSR). Quantitative real‐time PCR (qPCR) analysis indicated that each member of the IRI gene family was induced after exposure to low temperature. Heterologous expression of IRI proteins enhanced freezing tolerance in host cells. A total of 28 IRI genes were isolated from Triticeae, and the evolutionary relationship of the IRI gene family in Triticeae and related species showed high interspecific specificity. The IRI genes from Aegilops, which is one of the progenitors of allohexaploid wheat, shared the closest relationship with those from Triticum. With high interspecific specificity in Triticeae, however, there was no specificity among different species of Triticum. Our results suggest that IRI genes have potential value to improve cold resistance of crops. 相似文献
10.
The objectives of this study were to determine genetics of Al tolerance and whether the Al tolerance observed is governed by the same gene. The lines ‘L‐7903’ and ‘L‐4602’ have been developed through breeding programme as Al‐tolerant lines. These lines showed maximum root regrowth and minimum accumulation of Al and callose as compared to sensitive genotypes (‘BM‐4’ and ‘L‐4147’). Al tolerance in the parents, F1, F2 and backcross generations was estimated using the regrowth of the primary root after staining and scoring of fluorescent signals. The F1 hybrids responded similarly to the tolerant parents, indicating dominance of Al tolerance over sensitivity. The segregation ratios obtained for Al tolerance and sensitivity in the F2 and backcross generations were 3 : 1 and 1 : 1, respectively. Test of allelism confirmed the same gene was conferring Al tolerance in both genotypes (‘L‐7903’ and ‘L‐4602’) as the F1 was also tolerant and no segregation of tolerant : sensitive was recorded. These results indicated that Al tolerance is a monogenic dominant trait that can be easily transferred to agronomic bases through backcross breeding technique. 相似文献
11.
Pooran M. Gaur Srinivasan Samineni Mahendar Thudi Shailesh Tripathi Sobhan B. Sajja Veera Jayalakshmi Durdundappa M. Mannur Adiveppa G. Vijayakumar Nadigatla V. P. R. Ganga Rao Chris Ojiewo Asnake Fikre Paul Kimurto Robert O. Kileo Nigusie Girma Sushil K. Chaturvedi Rajeev K. Varshney Girish P. Dixit 《Plant Breeding》2019,138(4):389-400
Chickpea (Cicer arietinum L.) is a dry season food legume largely grown on residual soil moisture after the rainy season. The crop often experiences moisture stress towards end of the crop season (terminal drought). The crop may also face heat stress at the reproductive stage if sowing is delayed. The breeding approaches for improving adaptation to these stresses include the development of varieties with early maturity and enhanced abiotic stress tolerance. Several varieties with improved drought tolerance have been developed by selecting for grain yield under moisture stress conditions. Similarly, selection for pod set in the crop subjected to heat stress during reproductive stage has helped in the development of heat‐tolerant varieties. A genomic region, called QTL‐hotspot, controlling several drought tolerance‐related traits has been introgressed into several popular cultivars using marker‐assisted backcrossing (MABC), and introgression lines giving significantly higher yield than the popular cultivars have been identified. Multiparent advanced generation intercross (MAGIC) approach has been found promising in enhancing genetic recombination and developing lines with enhanced tolerance to terminal drought and heat stresses. 相似文献
12.
13.
André M. Almeida Enrique Villalobos Susana S. Araújo Barbara Leyman Patrick Van Dijck Luís Alfaro-Cardoso Pedro S. Fevereiro José M. Torné Dulce M. Santos 《Euphytica》2005,146(1-2):165-176
Summary Trehalose (a non-reducing disaccharide) plays an important role in abiotic stress protection. It has been shown that using
trehalose synthesis genes of bacterial origin, drought and salt tolerance could be achieved in several plants. A cassette
harboring the AtTPS1 gene under the control of the CaMV35S promoter and the Bialaphos resistance gene was inserted in the binary plasmid vector
pGreen0229 and used for Agrobacterium-mediated transformation of tobacco (Nicotiana tabacum). T0 plants obtained were analyzed by PCR for the presence of AtTPS1 gene. Thirty lines were positive and seeds were germinated on media with 6 mg/l PPT to obtain T1 plants that were grown in
the greenhouse to obtain T2 seeds that were germinated on selective media. Lines which seeds showed a 100 % survival rate
were considered homozygous transgenic T1 lines. Three lines were selected and gene expression confirmed by northern and western
blots. Transgenic seeds were germinated on media with different concentrations of mannitol (0, 0.25, 0.5 and 0.75 M) and sodium
chloride (0, 0.07, 0.14, 0.2, 0.27 and 0.34 M) to score their tolerance to osmotic stress. Assays were conducted to test the
tolerance of transgenic plants to drought (measurement of water percentage as a consequence of water withdrawal), desiccation
(measurement of water loss as a consequence leaf detaching) and temperature stresses (germination at 15 ∘C and 35∘C). Transgenic tobacco plant lines registered higher germination rates under osmotic and temperature stress situations than
did wild-type plants. Responses to drought and desiccation stresses were similar for all plant lines. It can hence be suggested
that the heterologous expression of TPS1 gene from Arabidopsis can be used successfully to increase abiotic stress tolerance in model plants and probably in other crops. 相似文献
14.
Wagner Rodrigo de Souza Nelson Geraldo de Oliveira Felipe Vinecky Ana Paula Ribeiro Marcos Fernando Basso Raphael Augusto das Chagas Noqueli Casari Brbara Andrade Dias Brito da Cunha Karoline Estefani Duarte Thaís Ribeiro Santiago Polyana Kelly Martins Carlos Eduardo Aucique‐Perez Sílvio Carlos Cristofoletti Júnior Alexandre Lima Nepomuceno Carlos Antnio Ferreira de Sousa Adilson Kenji Kobayashi Kazuo Nakashima Kazuko Yamaguchi‐Shinozaki Hugo Bruno Correa Molinari 《Journal of Agronomy and Crop Science》2019,205(6):545-553
15.
Yohei Koide Juan Pariasca Tanaka Terry Rose Ayumi Fukuo Kunihiko Konisho Seiji Yanagihara Yoshimichi Fukuta Matthias Wissuwa 《Plant Breeding》2013,132(3):259-265
Phosphorous (P) deficiency is a major yield limiting factor in rice (Oryza sativa L.) production. The interspecific New Rice for Africa (NERICA) varieties combine general stress tolerance from African cultivated rice (Oryza glaberrima Steud) with characteristics associated with high yield from O. sativa. However, little is known about their ability to tolerate P deficiency. Here, we examined the variation for tolerance to P deficiency among the 18 upland NERICAs and their parents in multi‐year field experiments. The good performance under P deficiency of the O. glaberrima parent CG 14 and some NERICAs suggested that these tolerant NERICAs contain loci associated with P deficiency tolerance inherited from CG 14. Additionally, four QTL clusters for P deficiency tolerance were detected on chromosomes 4, 6 and 11 using F3 lines derived from the cross between the P deficiency tolerant variety NERICA10 and a Japonica‐type sensitive variety ‘Hitomebore’. These QTLs represent the first step in identifying stress tolerance genes from O. glaberrima that could subsequently be used to enhance P deficiency tolerance in O. sativa. 相似文献
16.
Overexpression of a small heat‐shock‐protein gene enhances tolerance to abiotic stresses in rice
下载免费PDF全文
![点击此处可从《Plant Breeding》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rice (Oryza sativa L.) is one of the most important crops in the world which survives from various abiotic stresses in natural environments with specific stress‐involved genes expressed. Plant sHSPs (small heat‐shock proteins) were reported to respond to abiotic stresses. To improve the understanding of sHSPs in rice, we characterized heat‐shock‐protein gene OsHSP18.6 here. OsHSP18.6 could be induced by diverse stresses, such as drought, salt and cold, especially under heat. The gene was found expressed in root, stem, leaf, internode and spikelet. Overexpression of OsHSP18.6 results in increased thermotolerance and exhibits universal tolerance to stresses tested, including heat, drought, salt and cold. Lower levels of malondialdehyde (MDA) and greater activities of catalase (CAT) and superoxide dismutase (SOD) were observed in OsHSP18.6‐overexpression rice under heat and drought. OsHSP18.6‐overexpression lines indicated decreased sterile rates under hot weather without remarkable changes in most of other agronomic traits compared with wild‐type plants. 相似文献
17.
Evaluation of Cold Tolerance in NERICAs Compared with Japanese Standard Rice Varieties at the Reproductive Stage
下载免费PDF全文
![点击此处可从《Journal of Agronomy and Crop Science》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C. M. Wainaina Y. Inukai P. W. Masinde E. M. Ateka H. Murage M. Kano‐Nakata Y. Nakajima T. Terashima Y. Mizukami M. Nakamura T. Nonoyama N. Saka S. Asanuma A. Yamauchi H. Kitano J. Kimani D. Makihara 《Journal of Agronomy and Crop Science》2015,201(6):461-472
New Rice for Africa (NERICA) is a general name for interspecific rice varieties derived from a cross between the high‐yielding Asian rice (Oryza sativa L.) between locally adapted African rice (Oryza glaberrima Steud.). Eight NERICAs were evaluated for cold tolerance (CT) at the reproductive stage and compared with their O. sativa parents and three Japanese standard rice varieties over 3 years. Cold tolerance was evaluated based on the filled grain ratio (FGR) after cold water irrigation. The FGR was greatly reduced by cold water irrigation. NERICA 1, 2 and 7 had higher FGR (51.9–57.9 %), while NERICA 6, 15 and 16 had lower FGR (6.2–14.5 %). NERICA 1, 2 and 7 were less affected by cold stress, with a 31 % mean reduction in FGR, while NERICA 6, 15 and 16 were greatly affected, with their FGRs being reduced by more than 80 %. NERICA 3 and 4 were moderately affected by cold stress, with about 45 % reduction rate in FGR. FGR significantly influenced the grain weights of the varieties with strong positive correlations (r = 0.83–0.91; P < 0.001), and thus, similar trends in grain weights were observed. Grain weights were reduced by 61.7–96.4 % under cold stress. NERICA 1, 2 and 7 showed significantly better performance than NERICA 3 and 4, while NERICA 6, 15 and 16 performed poorly under cold water irrigation. The Japanese varieties Koshihikari (very tolerant) and Ozora (moderately tolerant) were more affected by cold water irrigation than NERICA 1, 2 and 7. On the basis of the mean reduction rate (%) in FGR under cold stress, the varieties were classified as follows: NERICA 1, 2 and 7 as tolerant; NERICA 3 and 4 as moderately tolerant; and NERICA 6, 15 and 16 as susceptible to cold stress. However, NERICA 7 grain yields were lower under cold stress due to both greatly reduced number of panicles per plant and number of spikelets per panicle. Therefore, NERICA 1 and 2 are suitable candidates for production in the highland regions of East Africa and should be promoted for production. 相似文献
18.
Homozygous transgenic cotton (Gossypium hirsutum L.) plants that accumulated glycinebetaine (GB) in larger quantities were more tolerant to salt than wild-type (WT) plants.
Four transgenic lines, namely 1, 3, 4, and 5, accumulated significantly higher levels of GB than WT plants did both before
and after salt stress. At 175 and 275 mM NaCl, seeds of all the transgenic lines germinated earlier and recorded a higher
final germination percentage, and the seedlings grew better, than those of the WT. Under salt stress, all the lines showed
some characteristic features of salt tolerance, such as higher leaf relative water content (RWC), higher photosynthesis, better
osmotic adjustment (OA), lower percentage of ion leakage, and lower peroxidation of the lipid membrane. Levels of endogenous
GB in the transgenic plants were positively correlated with RWC and OA. The results indicate that GB in transgenic cotton
plants not only maintains the integrity of cell membranes but also alleviates osmotic stress caused by high salinity. Lastly,
the seed cotton yield of transgenic lines 4 and 5 was significantly higher than that of WT plants in saline soil. This research
indicates that betA gene has the potential to improve crop’s salt tolerance in areas where salinity is limiting factors for agricultural productivity. 相似文献
19.
Fluorimetric Determination of the Genetic Variability Existing for Chilling Tolerance in Sweet Sorghum and Sudan Grass 总被引:1,自引:0,他引:1
M. Havaux 《Plant Breeding》1989,102(4):327-332
Chilling temperatures drastically inhibited the photochemical quenching of chlorophyll fluorescence (qQ) measured in intact leaves photosynthesizing under steady-state conditions. This effect appeared, however, to be characteristic of chilling-susceptible plant species and was not observed in plants which are known to be chilling-tolerant, indicating that the measurement of qQ can serve in practice to estimate rapidly the relative chilling tolerance of crop plants. A large number (28) of sweet sorghum (Sorghum bicolor) and Sudan grass (Sorghum sudanense) genotypes were screened for chilling tolerance using this rapid qQ method. Although sweet sorghum and sudan grass obviously behaved as chilling-sensitive plants, a considerable variation for chilling susceptibility was observed among the different genotypes tested. Some sweet sorghum varieties, such as ‘Dale’ and ‘Keller’, and most of the sudan grass varieties appeared to possess a certain degree of resistance towards low temperature stress, indicating the existence of useful germplasms in Sorghum for improving stress tolerance. 相似文献
20.
Josias C. Faria Paula A. M. R. Valdisser Elsa O. P. L. Nogueira Francisco J. L. Aragão 《Plant Breeding》2014,133(5):649-653
Bean golden mosaic virus (BGMV) is the causal agent of bean golden mosaic of common beans. A transgenic bean line that has been developed based on RNA interference to silence the BGMV rep gene showed immunity to the virus. Crosses were done between the transgenic line and six bean cultivars followed by four backcrosses to the commercial cultivars ‘Pérola’ and ‘BRS Pontal’. The transgene locus was consistently inherited from the crosses analysed in a Mendelian fashion in the segregating populations. The disease resistance reaction co‐inherited with the transgene. Nevertheless, the expression of disease resistance displayed a dosage effect phenomenon in the F1 generation. The analysis of the homozygous near‐isogenic lines in field conditions, under high BGMV disease incidence, indicated that the transgenic lines were completely resistant. These results show the strength of the disease resistance obtained, the stability of the trait across generations and its usefulness in the management of a disease for which there is no reported Phaseolus germplasm with immunity. 相似文献