香椿离体快速繁殖研究

本文以太和县香椿品种黑油椿为试材进行香椿离体快速繁殖研究,结果表明:茎尖最佳诱导培养基为MS+BA3.5 mg/L+NAA0.15 mg/L+GA30.1 mg/L;增殖培养基为MS+BA1.5 mg/L+NAA0.08 mg/L+GA30.01 mg/L;生根培养基为MS+IBA0.1 mg/L。初代接种时添加VC300 mg/L,PVP400 mg/L,可以减弱初代接种褐变率,提高接种成活率。     

子爵白掌的离体快速繁殖

介绍了子爵白掌外植体的采集、消毒,最佳启动、分化、生根培养基,组培苗的移栽、上盆等一系列快速繁殖配套技术。     

葡萄组织培养离体快速繁殖

葡萄品种繁多,基因型高度杂合,育种周期长,在有性或无性繁殖的过程中易造成基因型分离,降低品种价值.而通过顶芽、腋芽增殖的途径实现葡萄离体快速繁殖,简便易行,受基因型影响小,遗传性稳定,苗发育正常健壮容易移栽.我们在不断试验的基础上,实现了每月2.5～4.6的增殖倍数,短期内可生产大量优质组培全株苗,这将为缓解苗木紧缺,发挥良种栽培的增益效果,起到促     

松树微体快速繁殖的激素调控

从理论和实践二个方面,论述了激素对松树微体快速繁殖的调控。返幼的针叶束是成龄优树微型繁殖的良好外植体。激素的种类、浓度和处理方式是影响松树器官分化的重要因子。细胞分裂素和生长素,对提高营养繁殖的速度是必需的。最近研究表明,菌根对松树根系的发育和生长是重要的。     

大莺歌凤梨的离体培养快速繁殖试验

大莺歌凤梨组培快繁研究的结果表明：以茎尖或腋芽茎段为外植体,在培养基MS(或1／2MS) NAA1mg／L(或2,4-D2mg／L) 6-BA1～5mg／L上均可诱导芽分化,其中以1／2MS NAAlmg／L 6-BA5mg／L效果最好,培养43d可诱导出芽,分化频率50％;在培养基1／2MS NAA0．5mg／L 6-BA1mg／L上继代培养的增殖速率最高,培养30d芽的平均增殖倍数为7．4倍,降低6-BA浓度或提高NAA浓度有利于培养壮苗;1／2MS IBA1．0mg／L 1g／L活性炭的培养基诱导植株生根效果最好,30d生根率100％,平均每株苗生根3．5条,平均根长1.8cm;生根试管苗移栽于泥炭土：珍珠岩=1：1的基质,成活率约70％。软腐病是影响移栽成活率的主要因素,通风和对基质进行消毒可减少软腐病的发生。     

白玉兰离体培养和快速繁殖

试验建立了白玉兰离体繁殖体系。种子为外植体 ,初代培养基为 :B5 +BA0 .5mg/L +NAA0 .0 5mg/L + 30g/L糖 +琼脂 6g/L ,萌发率 85 %。继代培养基为 :B5 +BA1.5mg/L +NAA0 .15mg/L + 30g/glucose/sucrose + 6g/Lagar+Vco0 .5g/L ,月增殖率可达 5 .0± 0 .1。生根培养为 :1/2B5+NAA0 .5mg/L +AC1.0mg/L +VB2 0mg/L。以试管苗茎段、根段做外植体 ,培养基B5 +BA2mg/L+ 2 .4 -D0 .5mg/L +VC0 .5g/L ,可获得少量愈伤组织 ,继代存活率仅为 30 %。没有再分化成苗     

菊花的快速繁殖

菊花为菊科、菊属宿根性花卉植物。原产我国，栽培始于周、秦，迄今已有 3 000多年历史，为世界名贵花卉之一。为了更好地满足市场的需求，通过试管组织培养，可以达到快速繁殖的目的。 　　选择无病虫害的花蕾作外植体。花蕾先用 70％的酒精浸泡 15秒钟～ 20秒钟，再用 0 2％的升汞液浸泡 10分钟～ 15分钟，然后用无菌水冲洗 3～ 5次。剥去萼片并将花蕾纵切 4份，每瓶接种 1个花蕾进行培养。 　　均以 MS作为基本培养基，并根据不同培养环境的需要添加 NAA、 BA等成份。每升培养基附加蔗糖 30g，琼脂 7g， PH值调至 5 8。在温度为 …     

桐柏大枣组织培养快速繁殖技术研究

以优良枣树品种桐柏大枣为试材，对适宜的组织培养快速繁殖条件进行了研究，基本培养基为ＭＳ，筛选出了该品种的最佳启动、增殖、生根培养基，分别为：ＢＡ１．０＋ＩＢＡ０．０１￣０．１，ＢＡ１．５＋ＩＢＡ０．５＋ＧＡ０．５，ＢＡ０．０１＋ＩＢＡ２．０＋ＩＡＡ０．５，同时还取得了一些其他有指导价值的结果。     

仙人球的组织培养与快速繁殖

以全盛球种仙人球的茎切段和整个子球为外殖体进行试管培养，结果表明，在外殖体的切口处可诱导形成愈伤组织，在其刺座上的疣突处可直接产生小球体。经试验筛选出最适宜的培养基为：不定芽(小球体)诱导，MS+6-BA 8mg/L+KT 1mg/L+NAA 0.1mg/L；从生芽分化和继代，MS+6-BA 3.5 mg/L+KT 0.5mg/L+NAA 0.1 mg/L；生根，1/2MS+NAA 0.1mg/L+IBA 0.1mg/L+活性炭0.5%。     

不同激素和基质对黑穗醋栗扦插繁殖的影响

为了提高黑穗醋栗苗木繁育质量,分别以‘黑风’和‘黑布劳德’两个优良品种为试验材料,就枝条的不同部位(上部、中部和下部)、不同基质(河沙、蛭石、河沙+蛭石)和不同种类(IBA、NAA、ABT)、不同浓度(100、200、300 mg/kg)的激素处理对黑穗醋栗扦插生根的影响情况进行了嫩枝扦插和硬枝扦插试验。结果表明:以下部嫩枝扦插的生根率为最高;河沙与蛭石的混合基质适用于黑穗醋栗的硬枝扦插,以此混合基质扦插的生根率可达81%;每种激素处理的生根率,均以200 mg/kg浓度处理的为最高,其中以200 mg/kg的ABT处理的生根率达到82%,而当激素浓度升高到300 mg/kg时,生根率反而下降。     

不同无性繁殖措施对油茶苗木质量的影响

对高产油茶开展了不同无性繁殖方法的苗木质量比较研究,结果表明:长穗扦插育苗的成活率与苗木平均高生长高于短穗,但抽梢数、抽梢率则低于短穗;长穗扦插处理以生根粉浓度500 mg/L,浸入时间10 s,扦插入土1/2的成活率与抽梢率最高。扦插苗和嫁接苗适宜生产密度均为280株/m2;立地条件与管理水平影响油茶扦插苗的成活率、苗高、开花率及花苞数量;5月中旬生产嫁接苗的成活率较高,苗木生长最好;在油茶嫁接苗生产中宜清除花芽。     

In vitro clonal propagation of mature Tectona grandis through axillary bud proliferation

An in vitro clonal propagation procedure for mature Tectona grandis (teak) trees is described. Multiple shoots were induced from nodal segments through axillary bud proliferation. A shoot multiplication rate of 6.33 was achieved on Murashige and Skoog's (MS) medium supplemented with 10 μM 6-benzyladenine BA and 1 μM 1-naphthalene acetic acid (NAA) during every subculture cycle of 4 weeks. In vitro raised shoots could be successfully rooted (66.66%) on liquid MS medium supplemented with 15 μM NAA, with 1.60 roots per shoot, every 6 weeks of culture. In vitro hardening was carried out in sand soaked with half-strength MS medium (organic free). The plantlets were acclimatized first in a mist chamber and then in polybags in a mixture of soil, sand, and farmyard manure (1 : 1 : 1 v/v) in a shade house.     

Mini-cuttings technique: a new ex vitro method for clonal propagation of sweetgum

An efficient production system, which can quickly produce high quality plants, is shown in this study of Liquidambar styraciflua. The mini-cuttings technique was used for ex vitro clonal propagation of three sweetgum clones. The semi-hydroponics system for production of propagules was also tested, and the concentration of indole-3-butyric acid (IBA) for rooting was evaluated. Three adult clones, produced according to conventional cutting processes, were used as mini-stumps and maintained in semi-hydroponics systems of medium size sand. Ten collections of sprouts were prepared to evaluate the production of semi-hydroponics systems (mini-clonal hedges). Sprouts from collection number six were used at different concentrations of IBA solution (0; 500; 1,000; 3,000 and 6,000 mg l⁻¹) to test the mini-cuttings technique. The high survival rates of mini-stumps after ten collections (96.3%), and high annual production per square meter (~2,953 mini-cuttings) showed that the mini-clonal hedges are viable to conduct sweetgum mini-stumps. The formation of 62.5% of healthy plants after hardening under outdoor conditions showed that sweetgum could be successfully propagated by the mini-cuttings technique.     

杉木无性系繁殖技术

杉木是河南省南部山区主要用材造林树种 ,具有速生、丰产的特点。为保证培育出优质苗木 ,实现林分的快速生长 ,我们经过对杉木优树选择 ,进行无性系的扦插繁殖工作 ,通过实验对比 ,扦插苗的成活率在 95 %以上 ,当年生苗高 30ｃｍ ,侧须根发达 ,苗木生长健壮 ,成本比实生苗降低 2 0 % ,效果很好。1　建立采穗圃1.1　圃地选择选择背风、向阳、光照条件充足 ,水资源丰富 ,紧邻扦插育苗地 ,土质肥沃 ,土壤疏松 ,排水良好的地块。山地应坡度平缓 ,一般不超过 2 0° ,平地前作是茄子、辣椒、烟叶地不适宜 ;谷地宜选择东西走向。1.2　整　地秋、冬…     

In vitro clonal propagation of Gardenia latifolia Ait.: a toy making woody tree

An efficient, in vitro clonal propagation protocol has been established for Gardenia latifolia Ait. using mature nodal explants on Murashige and Skoog (MS) medium fortified with cytokinins (BA/Kn/2-iP) (1.0–5.0 mg l^?1) in combination with auxin IAA (0.5 mg l^?1). Maximum bud break (87 %) with shoot number (7.2 ± 0.26) observed on MS medium supplemented with BA (4.0 mg l^?1) and IAA (0.5 mg l^?1). Maximum number of shoots (30 ± 0.46) with shoot length of (0.9 ± 0.03 cm) observed on MS medium supplemented with BA (2.0 mg l^?1), Kn (2.0 mg l^?1) and IAA (0.5 mg l^?1). Further elongation of shoots (3.5 ± 0.06 cm) was achieved on MS medium supplemented with BA (1.0 mg l^?1) and IAA (0.1 mg l^?1). About 70 % of root induction occurred in half-strength MS medium supplemented with IBA (4.0 mg l^?1) in 4–6 weeks. Further elongation of roots with average length (9.0 cm) was achieved in culture bottles containing vermiculite and ¼ strength MS salts. After their partial hardening in these bottles for 30 days they were transferred to pots containing a mixture of soil and vermicompost (1:1) for acclimatization. The acclimatized plantlets were established in the field successfully with 85 % survival rate.     

毛红椿无性系扦插繁殖试验研究

对毛红椿无性系采穗圃嫩枝进行了扦插繁殖生根试验。结果表明:(1)毛红椿无性系嫩枝扦插生根兼有愈伤组织生根和皮部生根2种生根类型,参试的10个无性系中有8个无性系以皮部生根类型为主(占参试无性系的80%),有2个无性系同时包含2种生根类型;(2)毛红椿根系效果指数以TC01无性系最高,为36.5,毛红椿无性系嫩枝生根能力与生根率之间呈显著的正相关关系;(3)在遮荫降温、农膜保湿及自然温度条件下,以6月、9月和10月上旬毛红椿嫩枝扦插生根率最高。     

红叶石楠''''红罗宾''''工厂化扦插育苗快繁技术

从快繁设施设备、扦插材料的准备、扦插育苗和扦插环境的控制等方面归纳红叶石楠工厂化扦插育苗快繁技术。目前,江苏农林职业技术学院采用本技术已繁育红叶石楠苗500余万株,并向江苏及周边省市示范推广。     

An improvised in vitro vegetative propagation technique for Bambusa tulda: influence of season,sterilization and hormones

Season and concentration of sterilizing agents play a significant role for establishment of aseptic in vitro shoot cultures and sprouting of nodal explants from field growing culms of bamboo species.In the present investigation the nodal segment explants of Bambusa tulda Roxb collected in different seasons and treated with various concentrations of HgCl_2 showed significant variation in aseptic culture establishment and bud break.The rainy season(July–August) recorded with highest of 78% aseptic culture establishment whereas autumn recorded with lowest 46%.Summer and winter seasons emerged to be the best period, registering60% in vitro bud break.On the other hand, the autumn season had the lowest value for bud break,i.e.42%.Among different doses of sterilizing agent tried,HgCl_2 0.1% found to be suitable for maximum aseptic culture establishment(66%) as well as bud break(59%).However, among the interactions, summer season and the dose of 0.1% HgCl_2 exhibited maximum of 73% response for both aseptic culture establishment and bud break.MS medium(liquid) enriched with 5.0 μM BA+5.0 μM Kn[Kinetin(N6-Furfuryladenine)] with additional supplementation of 100 μM glutamine+0.1 μM IAA supported a maximum in vitro shoot multiplication of 4.75 fold.The proliferated shoots were successfully rooted on MS medium(liquid) supplemented 40 μM coumarin.The plantlets transferred to the polythene bags showed 98% survival.