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1.
Endothelial-mesenchymal transition (EndMT) is a biological process through which endothelial cells change their endothelial phenotype into a mesenchymal or myofibroblastic phenotype with the expression of mesenchymal markers such as α-smooth muscle actin (α-SMA) and vimentin. When the pulmonary vascular endothelial cells are exposed to hypoxia and inflammatory stimulation, vascular smooth muscle cells in the outer and middle membrane accumulate through EndMT process, leading to pulmonary vascular remodeling and pulmonary arterial hypertension (PAH). Transforming growth factor β (TGF-β)/bone morphogentic protein (BMP) signaling pathways promote EndMT process by bone morphogentic protein receptor 2 (BMPR2) gene mutation which up-regulates high mobility group protein A1 (HMGA1) gene and contributes to protein expression such as Slug and Snail, thus resulting in PAH. In brief, TGF-β/BMP signaling pathways and related regulators play an important role in pulmonary vascular reconstruction and the formation of PAH.  相似文献   

2.
AIM: To study the relationship between the activity of phospholipase A2 (PLA2) and pulmonary structural remodeling with the model of chronic isobaric hypoxic pulmonary hypertension. METHODS: 29 healthy Sprague-Dawley rats were randomly divided into normal control group, chronic hypoxic group and hypoxia plus Polidatin (PD) group. By diameter, the arteries were divided into two groups: arteries of group I (30 μm-100 μm) and group II (101 μm-200μm). The mean pulmonary arterial pressure (mPAP) was measured by inserting a microcatheter into the pulmonary artery. The PLA2 activity was measured with modified microtitrimetic method. The pulmonary tissue and arterioles morphology changes were examined under light microscope. RESULTS: It was found that after 21 days hypoxia, the mean pulmonary arterial pressure (mPAP), the PLA2 activity in blood and lung homogenate increased significantly. The media thickness of group I arteries increased (P<0.01) while that of group II arteries had no significant changes. The ratio of media area and adventitia of both groups was raised. Under light microscope, it was observed that pulmonary vascular endothelium proliferated, media became thickening and adventitia matrix increased. Pretreatment with PD could attenuate the changes mentioned above. CONCLUSION:PLA2 plays an important inducing role through promotion of the pulmonary vascular structural remodeling in the formation of chronic hypoxic pulmonary hypertension.  相似文献   

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AIM: To investigate the effect of angiotensin-(1-7) [Ang-(1-7)] on the development of monocrotaline (MCT) induced pulmonary arterial hypertension and vascular remodeling.METHODS: 60 Sprgue-Dawely rats were randomly assigned into three groups: control group, PAH group and PAH+Ang-(1-7) group. Rats in PAH group and PAH +Ang-(1-7) group received 60 mg/kg MCT injection subcutaneously and after 24 h received either saline or 24 μg·kg-1·h-1 of Ang-(1-7) injection via osmotic minipumps for 4 weeks. These rats in control group were firstly injected saline subcutaneously and then received saline injection via osmotic minipumps.RESULTS: After 4 weeks, in PAH group, right ventricular systolic pressure (RVSP), right ventricular hypertrophy index (RVHI), percentage of wall thickness (WT%) and percentage of wall area (WA%) of pulmonary artery were significantly increased and NO concentration, the level of endothelial nitric oxide synthase (eNOS), eNOS Ser1177-phosphorylation were significantly decreased compared with control group. However, RVSP, RVHI, WT %, WA % were dramatically decreased in PAH +Ang-(1-7) group and NO concentration, the level of eNOS protein, eNOS Ser1177-phosphorylation were significantly increased compared with PAH group.CONCLUSION: These results suggest that Ang-(1-7) could prevent the development of monocrotaline induced pulmonary arterial hypertension and vascular remodeling, which appears to be associated with up-regulation of NO concentration and the level of eNOS protein, eNOS Ser1177-phosphorylation.  相似文献   

5.
Pulmonary hypertension induced by high pulmonary blood flow involves a variety of complex mechanisms, including endothelial damage, pulmonary artery smooth muscle relaxation-contraction disorder and vascular remodeling. Besides, the factor of ion channels in pulmonary artery smooth muscle cells is also highly correlated to vasoconstriction. In recent years, many studies have shown that activation of Ca2+-activated Cl- channels is responsible for the membrane depolarization of pulmonary artery smooth muscle cells, and plays an important role in the regulation of vascular tone and vasoconstriction. This article reviews the biophysical and pharmacological characteristics of Ca2+-activated Cl- channels as well as the influence of Ca2+-activated Cl- channels in high pulmonary blood flow-induced pulmonary hypertension.  相似文献   

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AIM:To study the relationship between the activity of phospholipase A2 (PLA2) and chronic hypoxic pulmonary hypertension. METHODS:29 healthy SD rats were randomly divided into normal control group, chronic hypoxic group and hypoxia plus Polidatin (PD) group. The model of rat chronic hypoxic pulmonary hypertension was made by method of intermittent isobaric hypoxia for 21 days. The mean pulmonary arterial pressure (mPAP) was measured by inserting a microcatheter into the pulmonary artery. RESULTS:After exposing hypoxia for 21 days, the mPAP, R/L+S, the PLA2 activity, TXB2, MDA in plasma and lung homogenate increased significantly, while 6-k-PGF, SOD decreased significantly. Pretreatment with PD could relieve the changes mentioned above.CONCLUSION:PLA2 plays an important inducing role through its metabolic products and the interactional radicals in the formation of chronic hypoxic pulmonary hypertension.  相似文献   

8.
AIM: To examine the alteration of pathologic structure and gaseous molecules in rats with pulmonary hypertension induced by high pulmonary blood flow.METHODS: Aortocaval shunting was produced for 11 weeks in rats, and pulmonary hemodynamics was evaluated.Pulmonary vascular micro- and ultra- structure was also examined.Meanwhile,the concentration of plasma nitric oxide (NO) and carbon monoxide (CO) was measured by spectrophotometry.The expression of endothelial nitric oxide synthase (eNOS) and heme oxygenase-1 (HO-1) in pulmonary arteries was detected by immunohistochemistry.RESULTS: After 11- week aortocaval shunting,pulmonary artery mean pressure was significantly increased.Muscularization of small pulmonary vessels and relative medial thickness and area of pulmonary arteries were obviously increased in shunting rats compared with controls.Ultrastructure of intrapulmonary arteries changed obviously in shunting rats.Meanwhile,plasma NO concentration was increased and eNOS expression in pulmonary artery endothelial cells was significantly augmented in rats of shunting group.Plasma carbon monoxide level and HO-1 expression in puomonary artery smooth muscle cells,however,were not altered in shunting rats.CONCLUSIONS: Pulmonary vascular structural remodeling is the important pathologic basis of pulmonary hypertension induced by a left-to-right shunt,and NO other than CO might play an important regulating role in the development of high pulmonary blood flow-induced pulmonary hypertension.  相似文献   

9.
中国园艺学会第九届第8次常务理事扩大会决定,“中国园艺学会第七届青年学术讨论会”由山东农业大学园艺科学与工程学院和山东省园艺学会承办,将于2006年7月或8月在山东泰安举行。  相似文献   

10.
AIM: To investigate the changes of lung tissue and structure on bone marrow mesenchymal stem cells (MMSCs) transplantation to treat the pulmonary arterial hypertension (PAH) in rats.METHODS: MMSCs cells were collected from bone marrow of SD rat’s femoral’s tibial bones, mix cultured with Hoechst 33342 fluorescence dye in vitro. Sixty healthy male Sprague-Dawley rats (weighing 180 g±5 g) were randomly divided into 3 groups: normal control group (group C), MMSCs transplanted group (group M), pulmonary model group (group H). The rats of the two latter groups were given a single subcutaneous monocrotaline (50 mg/kg) to induce the model of PAH. The rats of normal control group were injected respectively a single subcutaneous isotonic Na chloride (6 mL/kg). The three groups were fed in the same condition. After 3 weeks, 5×109 cells/L MMSCs with l mL phosphate-buffered saline were infused into the rats respectively in group M by sublingual vein and 1 mL L-DMEM was administered in group H. Nothing was given to group C. The viability, right ventricle systolic pressure (RVSP), right ventricle hypertrophy index, the microstructure and ultrastructural changes of small pulmonary vascular were observed after 28 d.RESULTS: The viability of administrated MMSCs 28 d after PAH was 100% in transplanted group, and it nearly completely prevented the increase in right ventricular systolic pressure (RVSP) compared to PAH alone [(32.20±2.32)mmHg vs (48.30±1.56)mmHg, respectively, P<0.05], right ventricle hypertrophy index also degraded (38.80%±3.24% vs 45.10%±3.43%, respectively, P<0.05). The pulmonary arteries remodeling and ultrastructural changes of lung, such as blood gas barrier, chondriosome, osmiophilic lamellar body and so on, were improved significantly in MMSCs transplanted group. The changes were similar to the results of normal control group, but they were better than that in pulmonary arterial hypertension model group, which had conspicuous significance of statistics. MMSCs labeled with Hoechst 33342 were observed that they permanent planted and differentiated into plentiful collateral circulations in lung observed by fluorescence microscope. The pulmonary arteries remodeling were attenuated effectively in MMSCs transplanted group. Pulmonary fibrosis and tumorous were not observed in rats of MMSCs transplanted group.CONCLUSION: These results indicate that MMSCs reduce and even reverse the progression of pulmonary arterial hypertension (PAH) induced by crotaline. It restores the structure and function of microvasculature with marked improvement of tissue and structure changes of the lung.  相似文献   

11.
AIM:To explore the pathogenic mechanism of high altitude pulmonary edema(HAPE).METHODS:Haemodynamic changes and effects of 100 percent oxygen breathing were measured by Swan-Ganz thermistor catheters, high altitude healthy volunteers were served as controls.RESULTS:The important features of haemodynamic changes in HAPE: (1)Pulmonary arterial pressure was raised; (2)Pulmonary arterial resistance and cardiac output were raised; (3)Pulmonary artery wedge pressures and right atrial pressure were normal; (4)Pulmonary arterial pressure and resistance were induced by oxygen breathing.CONCLUSIONS:The normal pulmonary artery wedge pressures with a high cardiac output indicated that HAPE was recognized as a form of noncardiogenic pulmonary edema. The pulmonary hypertension may play an important role in the development of HAPE.  相似文献   

12.
AIM:The aim of this study is to investigate the acute inhibitory effects of adrenomedullin(ADM1-52) on hypoxia pulmonary hypertension and its influence on systemic blood pressure.METHODS:Thirty-six male Wistar rats were divided into two groups. Eighteen were exposed to hypoxia for 21 days as hypoxic pulmonary hypertension group,and another eighteen were kept in ambient as control group. Each group were divided into three subgroups which were injected intravenously with ADM 0.1 nmol/kg, 0.3 nmol/kg, 1 nmol/kg, respectively,then hemodynamic parameters were recorded. Plasma cyclic adenosine 3,5,-monophosphate (cAMP) was measured by radioimmunoassay before,during and after injection of 0.3nmol/kg adrenomedullin(ADM1-52).RESULTS:Mean pulmonary arterial pressure (mPAP)in the hypoxia and control rats treated with ADM decreased, the fall in hypoxia rats is more obviously than control(P<0.05), ADM (0.1-0.3 nmol/kg) produced dose-related reductions in mPAP in hypoxia rats(P<0.05). Mean systemic blood pressure(mSBP) of the rats in both groups decreased in a does-dependent manner, and it was more obvious in control rats. Plasma cAMP is higher in hypoxia group than that in control group.CONCLUSION:ADM depresses the rat hypoxia pulmonary hypertension in short term through, at least partly, cAMP-dependent mechanism.  相似文献   

13.
AIM:To investigate the role of potassium channel expression alteration in chronic cigarette smoking-induced increase in pulmonary vascular responsiveness, the effect of chronic cigarette smoking on large-conductance calcium-activated potassium channel (BKCa) and voltage-dependent delayed rectifier potassium channel (Kv1.5) expression in rat pulmonary smooth muscle cells were investigated in vivo. METHODS:HE staining, immuno-histochemistry and in situ hybridization techniques were used. RESULTS: (1) Chronic cigarette smoking downregulates the protein and mRNA expression of BKCa in pulmonary arterial smooth muscles. (2) Chronic cigarette smoking downregulated the protein and mRNA expression of Kv1.5 in pulmonary arterial smooth muscles. (3) In big artery, BKCa decreased more makedly than Kv1.5, but in small artery, both of them decreased equally. CONCLUSION:Chronic cigarette smoking downregulates the levels of BKCa and Kv1.5 in rat pulmonary arterial smooth muscle cells in vivo, which maybe contribute to the mechanism of cigarette smoking-induced increase in pulmonary vascular responsiveness.  相似文献   

14.
KONG Chun-chu  DAI Ai-guo 《园艺学报》2006,22(11):2132-2137
AIM:To investigate relationship among phosphatidylinositol 3-kinase (PI3K) and hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in lung of rats with hypoxia-inducible pulmonary hypertension. METHODS:Forty male adult Wistar rats were randomly divided into five groups (eight rats in each group):control group (C group) and groups with hypoxia for 3, 7, 14 and 21 days (H3, H7, H14 and H21 group). Mean pulmonary arterial pressure (mPAP), right ventric hypertrophy index (RVHI) and vessel morphometry were measured. The levels of HIF-1α mRNA expression in lung tissue was measured by in siteu hybridization (ISH). The protein expression of HIF-1α,VEGF and phosphorylated protein kinase β (P-AKT) were observed by immunohistochemistry or Western blotting. RESULTS:mPAP increased significantly 7 days after hypoxia [(23.53±1.78) mmHg], peaked 14 days after hypoxia, then remained on the high level. Pulmonary artery remodeling index (extern diameter 100 μm) and RVIH became evident 14 days after hypoxia. Expression of P-AKT protein in control group was poorly positive, but was up-regulated in pulmonary arterial tunica intima and tunica media in all hypoxia rats. HIF-1α mRNA staining was poorly positive in control,hypoxia for 3 days and hypoxia for 7 days, but began to increase significantly 14 days after hypoxia (0.305±0.104, P<0.05), then remained stable. Expression of HIF-1α protein in control group was poorly positive, but was up-regulated in pulmonary arterial tunica intima in all hypoxic rats. In pulmonary arterial tunica media, the levels of HIF-1α protein was markedly up-regulated after 3 days (0.029±0.019, P<0.05 ), reached its peak 7 days after hypoxia (0.232±0.008, P<0.05), then tended to decline 14 days and 21 days after hypoxia. Expression of VEGF protein began to increase 7 days after hypoxia (0.188±0.018, P<0.05), reached its peak 14 days after hypoxia (0.238±0.017, P<0.05), then remained on the high level in pulmonary arterial tunica intima. Linear correlation analysis showed that P-AKT, HIF-1α mRNA, VEGF and mPAP were correlated with vessel the morphometry and RVHI (P<0.01). P-AKT was positively correlated with HIF-1α and VEGF (tunica intima). CONCLUSION:P-AKT, HIF-1α and VEGF are all involved in the pathogenesis of hypoxia-induced pulmonary hypertension in rats.  相似文献   

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AIM: To investigate the roles of Panax notoginoside (PNS) and ERK1/2 signaling pathway in the pathological process of chronic hypoxic hypercapnia pulmonary hypertension in rats.METHODS: The animal model of chronic hypoxic hypercapnia pulmonary hypertension was set up in 72 male Sprague-Dawley rats and the animals were randomly divided into 6 groups: normal (N) group, hypoxic hypercapnia for 3-day (H3d) group, hypoxic hypercapnia for 1-week (H1w) group, hypoxic hypercapnia for 2-week (H2w) group, hypoxic hypercapnia for 4-week (H4w) group and PNS treatment (Hp) group.The rats in Hp group were injected with PNS (50 mg·kg-1·d-1, ip) before placing the animals into the hypoxic hypercapnia chamber.The rats in other groups were injected with normal saline (2 mL/kg, ip).The morphological changes of the pulmonary artery were observed under microscope with HE staining.Western blotting was used to detect the protein expression of p-ERK.The protein levels of p-ERK in the lung tissues and pulmonary blood vessels were determined by immunohistochemistry.RESULTS: The ratios of WA/TA in H1w, H2w, H4w and Hp groups were higher than that in N group (P<0.05).The ratio of WA/TA in Hp group was obviously lower than that in H4w group (P<0.05).The protein expression of p-ERK was barely positive in N group, but was up-regulated in the pulmonary tissues in all hypoxic rats.Compared with N group, the protein level of p-ERK was markedly up-regulated in H3d group, reached its peak in H2w group, and tended to decline in H4w group (P<0.05).In pulmonary arterial tunica intima and tunica media, p-ERK protein was dramatically expressed in all hypoxic rats compared with the control animals (P<0.05).In the lung tissues, the protein level of p-ERK in Hp group was lower than that in H4w group (P<0.05).In pulmonary arterial tunica intima and tunica media, the protein level of p-ERK in Hp group was lower by 84.86% than that in H4w group (P<0.05).CONCLUSION: ERK1/2 as a signal transducer may play an important role in the development of hypoxia and hypercapnia induced pulmonary hypertension.PNS inhibits the expression of ERK1/2, thus attenuating the development of pulmonary hypertension and improving pulmonary vascular remodeling.  相似文献   

17.
AIM: To explore the mechanism of cilazapril inhibiting proliferation of pulmonary vascular and myocardial cells in hypoxic rats. METHODS: 30 male Wistar rats were used and divided into three groups: normal control (group A), intermittent hypoxia for 4 weeks (group B) and intermittent hypoxia for 4 weeks plus cilazapril treatment (group C). The cell proliferation and structural remodeling in pulmonary vasculature and myocardium during hypoxia were studied by biochemical analysis, radioimmunoassay, immunohistochemistry, terminal deoxyuridine tripnosphate nick end labeling and correlated with hemodynamic. RESULTS: (1) The mean pulmonary artery pressure (mPAP) and the right ventricle to left ventricle plus ventricular septum ratio (R/L±S) were significantly higher in the hypoxic rat than that in control animals, while increased thickness of the pulmonary vascular wall and vascular lumen with decrease in the caliber as well as myocardial hypertrophy were observed in hypoxic rats. (2) The proliferative index (PI) of pulmonary arteria and myocardium was significantly higher in group B and C than that in group A. The distribution of ET-1 positive cells was seen in pulmonary arterial wall and cardiomyocytes. The ET-1 immunoreactivity was group B>group C>group A by turns. (3) The concentrations of plasma endothelin-1 (ET-1) and angiotensin converting enzyme (ACE) were significantly higher in group B than that in group A. However, the ET-1 and ACE were significantly lower in group C than those in group B. (4) The ET-1 and ACE had a significant positive correlation with R/L+S, mPAP and PI, respectively. The multivariate linear regression analysis revealed that ET-1 and ACE were major factor affecting PI. CONCLUSION: The pulmonary vascular and myocardial structural remodeling are one of the pathogenesis accompanied with excessive cell proliferation in hypoxic pulmonary hypertension (PH). Cilazapril effectively prevents and treats the hypoxic PH by inhibiting cell proliferation and structural remodeling of pulmonary circulation, as induced by ET-1 and ACE.  相似文献   

18.
AIM: To investigate the feasibility of exogenous hepatocyte growth factor (HGF) gene transfection to promote pulmonary collateral angiogenesis, improve pulmonary perfusion and reduce pulmonary artery pressure in the rabbit model of pulmonary artery hypertension (PAH). METHODS: The model rabbits of PAH were randomly divided into control group, empty vector group and HGF gene transfection group. The rabbits in HGF gene transfection group were transfected with Ad-HGF via intratracheal instillation. Pulmonary hemodynamic indicators were monitored in the 4th week after HGF gene transfection. Density of pulmonary vessels was examined with double-labeling immunofluorescence (endothelial cells were labeled with anti-FⅧ and vascular smooth muscle cells were marked with anti-α-SMA). Double-labeling immunofluorescence of FITC-lectin and anti-α-SMA was also performed to evaluate the pulmonary blood perfusion. RESULTS: Four weeks after transfection, the density of pulmonary arterioles of the rabbits in HGF gene transfection group was higher than that in control group and empty vector group (P<0.05), which was confirmed by double-labeling immunofluorescence. Pulmonary blood perfusion in HGF group was significantly increased compared with that in the other two groups, in which pulmonary arterial stenosis and occlusion were observed. The mean pulmonary artery pressure in HGF transfection group was much lower than that in control group and empty vector group (P<0.05). CONCLUSION: Four weeks after intratracheal adenoviral-mediated HGF gene transfection, pulmonary collateral vessels and pulmonary perfusion increase, and the pulmonary artery pressure is effectively reduced.  相似文献   

19.
AIM: To explore the effects of hydroxylamine on the pulmonary arterial pressure in chronic hypoxic hypercapnic rats. METHODS: Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups (8 rats in each group): the normal control group (NC), hypoxic hypercapnia+normal saline group (NS), hypoxic hypercapnia+hydroxylamine group (HA). The animals in NS and HA groups were kept in the O2 (9%-11%) and CO2 (5%-6%) cabin, 8 h a day and 6 days a week for 4 weeks. Before entering the cabin, the rats in HA group were administered with 1 mL hydroxylamine (12.5 mg/kg) by intraperitoneal injection, while the rats in NS group were given intraperitoneal injection of 1 mL saline solution. The mean pulmonary arterial pressure (mPAP) was measured by external jugular vein cannulation. The heart was removed, and the right ventricle (RV) and the left ventricle plus the septum (LV+S) were dissected. The ratio of the wet weight of the RV to that of the LV+S was calculated. The changes of the pulmonary vascular construction were observed under optical microscope. The concentration of H2S in the plasma was measured with a spectrometer. The expression of cystathionine-γ-lyase (CSE) in the pulmonary arterioles and bronchi was measured by immunohistochemistry and RT-PCR. RESULTS: The values of mPAP, RV/(LV+S),vessel wall area/total area (WA/TA) and media thickness of pulmonary arterioles (PAMT) in NS group and HA group were significantly higher than those in NC group (P<0.05). The level of H2S in the plasma, the content of CSE protein and the expression of CSE mRNA in NC group were significantly lower than those in NS group (P<0.05). The values of mPAP, RV/(LV+S), WA/TA and PAMT in HA group were significantly lower than those in NS group (P<0.05). The level of H2S in the plasma, the content of CSE protein and the expression of CSE mRNA in HA group were significantly higher than those in NS group (P<0.05). CONCLUSION: Hydroxylamine may decrease the pulmonary arterial hypertension induced by chronic hypoxic hypercapnia in rats by increasing the level of H2S in the plasma, the content of CSE protein and the mRNA expression of CSE, thus improving the pulmonary vascular structural remodeling.  相似文献   

20.
AIM: To explore the role of basic-fibroblast growth factor (bFGF) in the development of pulmonary hypertension induced by hypoxia. METHODS: 1) The pulmonary arteries of SD rats with hypoxia for one and two weeks were isolated, from which the total RNA were extracted by acid guanidinium thiocyanate-phenol-chlorform .Then the levels of mRNA were measured by RT-PCR. 2) About 3mm-long arterial rings cut from SD rat pulmonary arterial stem were suspended between stainless steelhooks in chamber with warmed (37℃) Kreb's solution. Different concentrations of bFGF were added in a cumulative fashion into the chamber where the rings were suspended. The cumulative concentration response curve was obtained. RESULTS: 1)The levels of bFGF mRNA in pulmonary artery of rats with hypoxia were increased significantly compared with those that without hypoxia (2578±384 counts·min-1 (control) vs 5303±756 (hypoxia) for 1 week and 4054±547 (hypoxia) for 2 weeks, P all <0.05). 2) bFGF at concentrations ranged from 5.56×10-10~2.78×10-7mol/L caused dose-dependent contraction of vessel rings of rat pulmonary artery (r=0.695,P<0.05), with EC50 being 2.62×10-7mol/L. CONCLUSION: bFGF may play an important role in the hypoxic pulmonary hypertension.  相似文献   

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