虾池浮游植物初级生产力的研究

用黑白瓶测氧法对辽宁省庄河青堆水产公司虾池整个养殖期间（5～9月）浮游植物初级生产力进行研究。虾池浮游植物的初级生产力为（5．45±2．36）gO2／（m2·d），日P／B系数为2．39（0．67～6.66），浮游植物对太阳有效辐射的利用率为0．78（0．24～2．18）％，浮游植物毛产量到虾产量的转化效率为7．41％；回归分析表明，浮游植物生物量和太阳有效辐射量是决定虾池浮游植物初级生产力大小的主要因子。磷是虾池营养盐的第一限制因子，氮是第二限制因子。     

Determination of critical limits of soil nutrients for use in optimizing fertilizer rates for fish ponds in red, lateritic soil zones

Mesocosms containing lateritic soil from West Bengal, India and de-ionized water were treated with different amounts of nitrogen (N), phosphorus (P), and potassium (K) fertilizers. Concentrations of available N, P, and K in soils and gross primary productivity were measured for the mesocosms. Critical concentrations of the three nutrients were estimated by the graphical method of Cate and Nelson that has been used widely for establishing critical soil nutrient concentrations in fertilization research on terrestrial crop plants. Critical concentrations of the three nutrients were: available N, 200 mg kg⁻¹; available P, 13 mg kg⁻¹; available K, 80 mg kg⁻¹. Ponds sited in red, lateritic soil in West Bengal were treated monthly with 20, 15, and 3 kg ha⁻¹ application⁻¹ of N, P, and K, respectively. There was an increase in gross primary productivity when the critical concentrations of available N, P, and K established in the mesocosm study were attained in the pond soils. Fish production in fertilized ponds is positively correlated with gross primary productivity. Thus, the concept of critical nutrient concentrations in soils could be a valuable tool in pond fertilization research.     

生物渔肥对水泥池浮游生物种群的影响

试验探讨了施用生物渔肥(绿源生)后水体中浮游植物和浮游动物的变化。试验取2个水泥养殖池,其中一个为试验池,施入生物渔肥量为4 g/L,另一个为不施肥的对照池。试验结果表明:施用生物渔肥后试验池中的浮游植物在第2天开始迅速增长,第4天达到生长高峰,比对照池同期增长116.25%,硅藻门取代绿藻门成为优势种群;试验池中的原生动物在第4天达到最大值,比对照池增长16.18%,轮虫、枝角类,桡足类在第6天大量增长,比对照池分别增长30.40%、20.59%和17.46%;试验池浮游生物总量在施入渔肥后的第12天仍高于对照池。本试验条件下,生物渔肥对浮游植物和浮游动物的种类影响不大,但对浮游植物和浮游动物的密度影响较大。     

Determination of dosage of Azotobacter and organic fertilizer for optimum nutrient release, net primary productivity and fish growth in fresh water fish ponds

Four experiments were conducted in order to determine the optimum dosageof Azotobacter chroococcum vis-a-vis organic fertilizer(cow-dung) required for optimum pond productivity. Hydrobiological parameters ofpond water, Azotobacter survival (viable counts), netprimary productivity (NPP) and fish growth were monitored. Studies have revealedthat irrespective of the treatments, dissolved oxygen (DO) levels weresignificantly (P < 0.05) lowered on inoculating the ponds withAzotobacter. Alkalinity, O-PO₄,NO₃-N, turbidity, NPP, plankton population and fish growth weresignificantly (P < 0.05) enhanced in ponds inoculated withAzotobacter @ 100.0 ml pond^–1w^–2 in combination with cow-dung @ 10000 kgha^–1 y^–1. At higher or lower dosages offertilizers, the values in most of these parameters remained low. On the otherhand, total kjeldahl nitrogen and NH₄-N increased continuously. Ingeneral, viable bacterial counts decreased with increase in pH, however, therate of nitrogen fixation was not affected. Multivariate analysis of the data revealed a significantpositive correlation of nutrients (Total kjeldahl Nitrogen, NO₃-N andO-PO₄), with NPP and plankton populations. NH₄-N, however,showed a significant negative correlation with DO, NPP and plankton populations.Highest fish biomass and SGR also coincided with the highest NPP and planktonpopulations, revealing that a dose of 100.0 ml pond^–1w^–2 (for 25 m³ ponds) ofAzotobacter along with 10000 kg ha^–1y^–1 of cow-dung appears to be optimum for obtainingoptimum pond productivity and fish yield. Nutrients in the sediment(NO₃-N and O-PO₄) also followed similar trend. On theother hand, organic carbon increased continuously with each increase in thedosage of fertilizers. A decline in fish biomass and pond productivity at higherfertilizer dosages has been attributed to low DO, high NH₄-N and BOD.     

Effect of varying closes of organic and inorganic fertilizers on plankton production and fish biomass in brackish water fish ponds

The effects of four different doses of organic (cowdung) and inorganic fertilizers (single super phosphate: SSP) in combination were studied on plankton production, species diversity and fish biomass in saline and freshwater fish ponds. Physico-chemical factors of pond waters were also monitored. Alkalinity and nutrients increased with increase in the dose of fertilizers. Dissolved oxygen (DO) also remained sufficiently high up to the third treatment; however, levels declined significantly in the ponds receiving the fourth treatment (20 000 kg ha^-1 year^-1 of cowdung and 3000 kg ha^-1 year^-1 of SSP). The highest plankton population, species diversity and higher fish biomass was also observed in ponds which received the third dose of fertilizers (10 000 kg ha^-1 year^-1 of cowdung and 1500 kg ha^-1 year^-1 of SSP). However, a decline in these parameters was observed in ponds which received the highest (fourth treatment) dose of fertilizers. Nutrients remained slightly lower in brackish-water fish ponds. When species diversity values were compared, it was observed that, although values werte higher in freshwater ponds, their abundance (no. 1¹) remained lower than in brackish-water fish ponds. Similarly, fish biomass also remained significantly higher in brackish-water ponds than in freshwater ponds. From these studies, it can be concluded that a combination of 10 000 kg of cowdung + 1500 kg ha^-1 year^-1 of SSP appears to be the optimum dose.     

Effect of fish species combinations on water chemistry and plankton composition in earthen fish ponds

Fish-environment relationships in ponds with several different combinations of fish species are analysed. The study was carried out in 18 earthen fish ponds in a farm at Lohamei HaGetaot, Israel, during the 1986 fish culture season. Water was analysed for temperature, dissolved oxygen, pH, ammonium, nitrite, nitrate, and phyto- and zooplankton. Factor analysis was applied to the physico-chemical and plankton datasets to identify respectively the main ecological processes and species groups in the ponds. The general linear model was applied to analyse the effect of fish species combination and month on the ecological and plankton factors identified. The main source of water quality variability was accounted for by three factors: pond loading (CHF1), oxygenation of the water (CHF2), and pH effect on the second step of nitrification (CHF3). The main source of variability in the plankton composition was due to presence or absence of a blue-green algal bloom (PLF1). Another four plankton factors were identified. Each factor was differently affected by the fish species combination in the pond and the month. The complex nets of interactions between common carp, tilapia and mullet, and how these fish in different cominations affect pond water quality and food webs are discussed.     

烟台四十里湾叶绿素a和初级生产力的分布特征

2006～2010年5、8、10月,分别对烟台四十里湾近岸水域进行了30航次的综合调查,调查内容包括海水水质、浮游植物、叶绿素以及水文气象等要素。四十里湾近岸水域表层叶绿素a和初级生产力的季节与年际变化结果显示,浮游植物数量、叶绿素a含量和初级生产力一般在8月出现高值;仅2008年浮游植物数量最高值出现在10月,2009年叶绿素a含量和初级生产力最高值出现在5月。2008年10月浮游植物数量平均值为所有航次中最高,达到51.61×104cells/L,且各站位间差别显著(P<0.01);2007年10月最低,仅为0.02×104cells/L。2006～2009年,表层叶绿素a含量和初级生产力均值呈逐年增加趋势,2009年分别达到8.72μg/L和1481.42mg/m3·d;2010年(叶绿素a:6.13μg/L;初级生产力:733.91mg/m3·d)又降至与2008年相当。近5年,四十里湾浮游植物数量、叶绿素a含量和初级生产力均呈现湾东部高于西部的趋势,初级生产力与表层叶绿素a含量呈强正相关关系(R=0.921,P<0.01),并得出二者的回归方程式,这为海洋初级生产力的估算与评价提供了一种更简便的方法。     

The effect of fish species combination in fish ponds on plankton composition

Abstract. In continuation of an experiment carried out in 1983, the effect of polyculture of bottom-feeding fish (common carp. Cyprinus carpio L.; tilapia hybrids, Oreochromis nilolicus (L.) × O. aureus (Steindadner); and grey mullet, Mugil cephalus (L.) and a filter-feeding fish (silver carp. Hypopluhalmichtys molitrix (Valenciennes)) on the nannoplankton and netplanklon has been studied. This effect was analysed through principal component analysis. The results confirmed the linding of 1983 that the presence of silver carp in a pond, either alone or with bottom-feeding fish, results in a decrease in netplankton (phylo- and zooplankton) and an increase in nannoplankton which passes through the tiller apparatus of the fish. The effect of the silver carp seems to be related more to its numerical density than to its individual weight, since the effect diminished with the decrease in density during 1983–1985. It became least pronounced when the density was 400/ha. Bottom-feeding fish enhance the development of the larger nannoplankton by bringing up nutrients from the bottom, but reduce the number of the netplankton (mainly zooplankton) as compared to the control pond without lish. This effect was more apparent at a higher density of tilapia. but was masked when silver carp was present.     

有益微生物对海水养虾池浮游生物生态特征的影响研究

研究了有益微生物对海水虾池浮游生物生态特征的影响。通过对虾养殖系统中浮游动物与浮游植物进行为期3个月的监测,结果表明试验组虾池的浮游植物密度表现为前期低,中期迅速增长,后期达顶峰,并维持在70×106～160×106cell·L-1的水平,前期种类以硅藻类为主,后期则以绿藻类为主;同时试验池浮游动物的密度也保持稳定增长态势,并在养殖后期维持在顶峰水平,密度达到25×103～30×103ind·m-3,种类以广盐、适低盐的沿岸种为主,但优势种明显;对照池的浮游植物和浮游动物呈现前期低后期高的态势。其变化差异表现为对照组的浮游植物和浮游动物在后期的密度变动起伏较大,且出现较大比例的有害的兰藻类,而试验池的兰藻类只占较少的比例,说明芽孢杆菌群能抑制兰藻类的繁殖,促进有益藻类生长;浮游生物的多样性指数较低,低于自然海区,但种类均匀度则较高,与自然海区相当。     

循环水池塘与非循环水池塘中浮游生物的群落结构及其动态研究

研究了循环水对池塘中氮、磷含量、浮游生物种群结构及其相互关系的影响。结果显示:从循环塘中共鉴定出浮游植物93种,隶属6门47属;非循环塘中共鉴定出浮游植物100种,隶属6门48属,循环塘与非循环塘均以绿藻门种类最多,分别占各塘种类总数的40.86%、42%。两塘中浮游植物的密度和生物量分别为:循环塘为247.68～1133.31(×104ind/L)和3.07～10.83 mg/L;非循环塘为511.68～1773.3(×104ind/L)和4.48～17.34 mg/L。循环塘浮游植物的密度和生物量比非循环塘要低,且差异性显著(P<0.05)。循环水鱼塘中共检出浮游动物33属42种,非循环水池塘68属88种,在试验期间两塘中浮游动物的优势种主要是由原生动物和轮虫组成,其种数变化顺序为原生动物>轮虫>枝角类和桡足类。循环塘中原生动物和轮虫分别占浮游动物总种类数的52.38%、33.34%;而非循环塘中原生动物和轮虫分别占浮游动物总种类数的42.05%、48.86%。其中循环塘72～708.11 ind/L和0.41～1.69 mg/L;非循环塘152.9～208.20 ind/L和0.26～0.46 mg/L。循环塘浮游动物的密度和生物量显著高于非循环塘(P<0.05)。循环塘与非循环塘中浮游植物的Shannon-Wiener指数分别为介于1.39～1.62和1.16～1.48之间,两塘中浮游动物的Shannon-Wiener指数分别为介于0.50～1.95和1.08～1.45之间。结果表明:循环水对养鱼池塘水体中浮游生物的出现率、丰度和生物量影响较大。     

Application of Azotobacter enhances pond productivity and fish biomass in still water ponds

Two strains (derepressed-nitrogen fixing, Mac-27 and phosphate solubilizing, PS-21) of Azotobacter chroococcum were inoculated in fish culture ponds, singly and in combination with inorganic fertilizers (urea, single superphosphate–SSP). Physico-chemical parameters of pond waters, plankton production and fish biomass were studied. Inoculation of A. chroococcum (Mac-27) enhanced nitrogenase activity and rate of nitrogen fixation. A slight reduction in nitrogen fixation and nitrogenase activity was noticed when urea at 96 kg ha–1 y–1 was mixed with the biofertilizer (Mac-27). Inoculation of PS-21 enhanced phosphate solubilization, but Kjeldahl-nitrogen concentration values remained low in comparison with controls. On the other hand, inoculation of Azotobacter (either strain) enhanced the accumulation of ammonium-N, nitrite-N and nitrate-N. A significant (p < 0.05) reduction in dissolved oxygen (DO) concentration also took place when Azotobacter (both Mac-27 and PS-21) was inoculated in fish ponds. However, when used along with inorganic fertilizers, the reduction was not significant. The pH values were only slightly lowered when the phosphate-solubilizing strain (PS-21) of Azotobacter was inoculated. Inoculation of biofertilizer enhanced plankton production, net primary productivity and fish biomass. However, highest values in most of these parameters were noticed only in ponds that were treated with the higher doses of inorganic fertilizers (urea 192 kg and SSP 1500 kg ha–1 y–1). © Rapid Science Ltd. 1998     

光合细菌强化对精养鱼塘藻类群落结构的影响

通过定期向池塘投加光合细菌,研究了有益微生物强化对精养鱼塘浮游藻类群落结构的影响。结果显示:光合细菌强化塘(试验塘)藻类组成以绿藻、硅藻为主,养殖前期(5—6月初),以针杆藻、直链藻等占优势,中后期(7—9月)则以绿球藻、栅藻、盘星藻、小环藻、菱形藻等为优势种,水色呈淡绿、黄褐色等良好状态;而对照塘藻类组成在前期与试验塘没有明显差异,但在中后期出现明显变化,以微囊藻、颤藻、鱼腥藻、席藻等蓝藻占优势。试验塘藻类生物量显著低于对照塘(P<0.05),且对照塘生物量波动变化大,蓝藻数量所占比例可高达77.6%,远高于试验塘蓝藻比例(均值低于25.0%);此外,藻类Shannon-Wiener指数、Pielou指数试验塘均大于对照塘。结果表明:光合细菌的定期添加有效控制了蓝藻增值,保持了藻类多样性,使精养鱼塘藻相趋于稳定,有利池塘养殖。     

Effect of organic nitrogen and carbon mineralization on sediment organic matter accumulation in fish ponds

In aquaculture, ponds with high loads of organic inputs, organic matter accumulates at the bottom over time. Uneaten feed, senescent phytoplankton and faeces are the principal sources of accumulated material, but quantifications are scarce. The sedimented organic matter develops into a flocculent layer in which different processes transform the material into inorganic forms. A better understanding of factors influencing organic matter accumulation/decomposition in the sediment is needed to better understand and manage the dynamics of nitrogen in fish ponds. In this study, the rate of mineralization of organic nitrogen and the nitrogen flux between the sediment and the water column were measured. Organic matter accumulation in fish ponds was quantified, and the data were used to construct, calibrate and validate a dynamic simulation model of organic matter deposition/decomposition in fish ponds. The accumulating material consisted of dead phytoplankton, fish faeces and uneaten feed. Through model calibration, the proportion of these materials in the total accumulated organic matter was determined. In the model, gross photosynthetic rate was estimated from an empirical relationship with feed input. After calibration, the model was validated using independent data. The model simulated well the concentrations of organic carbon and nitrogen in the sediments but it may be developed further, especially by considering the effects of resuspension.     

Unravelling the diversity and assemblage of phytoplankton in homestead ponds of central coastal belt,Bangladesh

Homestead ponds may offer an ideal opportunity in contribution to household income, fish consumption through fish culture. Phytoplankton plays a key indicator in pond productivity. However, the phytoplankton communities in the homestead ponds are nearly undetermined in Bangladesh. This study summarizes the phytoplankton diversity, assemblage and ecological parameters of the homestead ponds in central coastal belt, Bangladesh, based on two seasons. The results revealed that significant differences (p < .05) were found in pH, DO, conductivity, TDS, nitrates, phosphates and sulphates except temperature in monsoon, and transparency in both seasons. A total of 34 phytoplankton genera representatives of five major groups were recorded such as Euglenophyceae (41.5%), Chlorophyceae (30.5%), Cyanophyceae (13%), Dinophyceae (10%) and Bacillariophyceae (5%). A significant difference (p < .05) in phytoplankton abundance was found in both seasons with the highest mean of 186.13 ± 129.34 × 10⁴ cells L^?1 in monsoon and the lowest mean of 144.40 ± 107.26 × 10⁴ cells L^?1 during winter. The diversity (H'), evenness (J'), richness (d) and dominance (D) ranged from 1.62 to 2.47, 0.52 to 0.97, 0.37 to 1.15 and 0.10 to 0.28 respectively. Based on SIMPER, the most dominant (>10%) genera for dissimilation within stations and seasons were Euglena spp., Phacus spp., Chlorella spp., Strombomonas spp. and Peridinium spp. ANOSIM results stated that 12 genera of phytoplankton were significant contributors based on average dissimilarity. Pearson's correlation coefficient and canonical correspondence analysis (CCA) revealed that phytoplankton abundance, diversity and community were mainly governed by transparency, dissolved oxygen and nitrates.     

Effects of seasonal variations and pond age on trace elements and their correlations with plankton productivity in commercial freshwater crayfish (cherax cainii austin, 2002) earthen ponds

Seasonal variations can affect the concentration of trace elements, and the change in their concentrations can affect the natural productivity of freshwater aquaculture ponds. Hence, we studied the seasonal variations of the 12 pre‐selected trace elements (Co, Cu, Fe, Mn, Zn, Se, Ca, Mg, P, S, Al, Si) and their relationships with primary and secondary productivity in two aged ponds, stocked with three different life stages of marron (Cherax cainii), for a period of 1 year. Trace element analysis was performed by using (Agilent, ICP‐OES). Except Co and Se, all trace elements, and primary and secondary productivity were influenced by seasonal variation. The pond age significantly influenced the concentrations of some trace elements. On a seasonal basis, trace elements were positively correlated with the plankton abundance, species diversity, and wet and dry plankton weights. Seasonal variations and pond age affected the dissolved concentrations of trace elements and plankton productivity.     

Role of organic fertilizers in walleye (Sander vitreus) production in plastic‐lined culture ponds

This study used six 0.04 ha plastic‐lined ponds to compare the effects of a fertilization regime using a one‐time initial application of an organic fertilizer (alfalfa pellets) with the current regime of weekly applications of organic fertilizers on the abundance and distribution of aquatic invertebrates and walleye, Sander vitreus, fingerling production. Walleye, 3–4 days post hatch, were stocked on 1 May 2002 and harvested on 6–7 June 2002. Throughout the growing season, a ratio of 7:1 total nitrate‐nitrogen to total phosphorus was maintained in all ponds regardless of the treatment. Once fry were stocked, ponds in Treatment #1 were fertilized weekly with organic fertilizer (alfalfa pellets; 112 kg ha^?1 week^?1) for a total of 795 kg ha^?1 pond^?1. Ponds in Treatment #2 only received an initial application of alfalfa pellets (112 kg ha^?1). Ponds in Treatment #1 had significantly higher ammonia and nitrate levels as well as higher chironomid larvae but not zooplankton compared with the other treatment. At harvest, walleye in the Treatment #1 ponds were significantly longer and heavier; however, the survival and relative weight were not significantly different. These results suggest that weekly applications of organics are important for the benthic food base and growth of fingerling walleye reared in plastic‐lined ponds.     

Effects of lining and drying on the chemical composition of sediments and influence of organic carbon on carp growth in aquaculture ponds

Chemical characteristics and P‐fractionation of sediments from the Deroua fish culture farm (Beni Mellal, Morocco) were analysed in 1998 and 1999. The high concentrations of organic N and organic C in the sediments reflect the high productivity of phytoplankton in the lined ponds. Despite the fact that these ponds were not frequently fertilized, inorganic P was more important in the sediments. The P released from CaCO₃≈P was the principal source of P for phytoplankton. The mineralization rate of organic C during the drying period was about 87 µg g^?1 day^?1 in the sediments. The mineralization rate of organic N seems to be less, compared with organic C. The experimental results indicate that the C:N ratio and texture of sediments could explain the immobilization of organic N. All forms of P remained almost unchanged after the drying period except for Fe(OOH)≈P. The ferric (oxy) hydroxides protect NaOH_ext‐P from the bacterial lyses and stabilize therefore their concentration. The inorganic P represented about 88% of Total P in the sediments and may influence the mineralization rate of organic P. The growth of silver carp were reduced in presence of high concentrations of organic C in sediments (> 30 mg/g). Compounds toxic to fish such as Fe²⁺, Mn²⁺, H₂S and CH₄ were then released in the water column.     

不同肥料对池塘细菌种群的影响

运用PCR-DGGE技术分析有机肥(干鸡粪)和化肥(碳酸氢铵和磷酸一铵)对鱼苗池塘中的理化因子和细菌数量及其种群结构的影响,以探讨细菌种群结构对不同肥料的响应。结果显示:施肥后池塘中NH4+-N、PO43--P、NO 3--N和NO 2--N等浓度增加,透明度降低,细菌数量、种群多样性和丰富度指数均增加;化肥增加溶氧和升高pH,有机肥却降低溶氧和pH。施肥后蓝细菌门(Cyanobacteria)和拟杆菌门(Bacteroidetes)种群成为优势种群,有机肥能增加厚壁菌门(Firmicutes)种群的相对丰度,而化肥却降低其相对丰度;β-和γ-变形杆菌纲种群的相对丰度在施入化肥后增加,有机肥却限制γ-和δ-变形杆菌纲种群,化肥明显降低放线菌门种群的相对丰度。池塘中理化因子、细菌种群数量及其结构因施入不同肥料发生相应的变化。     

The influence of ocean climate on coastal plankton and fish production

This study evaluates the influence of oceanic variability on plankton and fish production in a coastal upwelling system off southern Vancouver Island, British Columbia, Canada. A trophodynamics model is used to hind-cast plankton and fish production as estimated by empirical seasonal patterns in upwelling, sea surface temperature, solar radiation, and by biomasses of Pacific hake (Merluccius productus) and Pacific herring (Clupea pallasi). Simulation results indicate that interannual and longer-term variability in coastal plankton production occurs, and that the patterns are primarily determined by the dynamics in the initiation and intensity of coastal upwelling. Fish properties are greatly influenced by zooplankton, with simulated hake production being correlated with summer euphausiid production, and measured herring condition being correlated with autumn zooplankton production. An evaluation of transfer efficiencies indicates that interannual variability in the diatom-to-fish transfer is high (CV = 78%), with the majority of this variability occurring in the diatom-to-zooplankton component of the transfer. Seasonal dynamics of the diatom-to-zooplankton transfer result from the effects of upwelling on diatom production, and subsequent trophodynamic phasing with copepods and euphausiids.