一起蛋鸡曲霉菌病的病例报道

１９９９年１２月１６日,我市养鸡专业户张某,带病鸡前来我院诊治,禽主陈述,此群鸡已２３８日龄,品种海兰白共１１００羽,一直长势良好,于８月上旬开始产蛋,到１１月下旬产蛋率达８成多,１２月４日发现少数鸡有轻微的甩鼻、打咯、咕噜等异常症状,自以为是新城疫抗体偏低,遂让鸡群饮服６倍量的克隆３０疫苗观效,５天后又投红霉素饮水,氟哌酸拌料,连用５天,均无效,而且出现呼吸困难,伸颈张口,冠髯发绀等症状,大群鸡采食减少,饮欲增加,拉白色稀粪严重。随着病情发展,鸡冠逐渐苍白、萎缩、变小,以至脱水,衰竭死亡;产蛋…     

蛋鸡感染前殖吸虫的病例报道

某户饲养720羽商品伊沙蛋鸡,开产后笼养,30周龄时产蛋逐渐下降,并每天见有薄壳蛋和小蛋产出,病鸡逐渐消瘦,经对可疑病鸡剖检和异常蛋检查,找到多条前殖吸虫虫体。 发病情况:此批蛋鸡,育雏阶段在舍内饲养,成活率达98％。90～120日龄阶段白天圈养于靠近池塘的空地上。就诊时已有25只大腹鸡死亡,还有5只大腹病鸡。病鸡呈现腹部膨大,叩诊有波动感,腹部羽毛脱落、潮红,泄殖腔突出,病鸡不喜活动,行走呈“鸭     

一起蛋鸡群感染禽流感疫情的案例分析

<正>2012年4月,本市S区从产蛋鸡群中检测出高致病性禽流感(HPAI)病原学阳性鸡群,随后大范围发生和流行HPAI疫情[1]。疫情发生期间,S区T村养鸡户积极采取防控措施,但仍然不可避免的发生了疫情。由于复杂因素的影响,T村发生HPAI疫情的发病鸡群当时未进行无害化扑杀处置,我们对T村免疫鸡群发生HPAI的情况进行了追踪观察。1 T村养鸡和防控基本情况经统计,至5月10日,T村共有养鸡户24户,     

一起肉毒梭菌毒素中毒的病例报道

1995年8月1日,某鸡场养在同一层笼的90只12周龄鸡中有24只昏迷、软瘫、头颈无力、时而仰起、时而前垂、羽毛脱落凌乱,而其他鸡只完全正常。临诊检查,体温41℃,反射正常,剖检无肉眼可视病变,疑为高温中暑。静注10％葡萄糖生理盐水及维生素C无效。病鸡于3日内陆续死亡。后检查鸡笼,发现有已被啄食的腐烂鸡尸,疑为肉毒梭菌毒素中毒。随后进行禽眼睑闭合试验,确诊为该病。     

小心弱毒型禽流感卷土重来

几年前，弱毒型禽流感（低致病性禽流感H9N2亚型）在我国发生并流行，导致大批蛋鸡发病．产蛋下降，并且造成了不同程度的死亡，当时肉鸡也有零星发生。H9N2亚型禽流感给养鸡户一个措手不及，由于当时没有这方面的研究，更没有疫苗．因此疫情出现后给广大养鸡户造成了巨大的经济损失。     

一起蛋鸡意外事故的报道

发病情况2010年10月,一蛋鸡饲养户由于管理不善,导致了一次意外事故的发生,造成了不该出现的经济损失。10月8日上午,禽病专家门诊来了一位济阳的蛋鸡养殖户,用纸盒子盛着三只活鸡来就诊。主诉:共存栏1500只蛋鸡,已经180日龄。鸡舍为东     

一起蛋鸡意外事故的报道

发病情况2010年10月,一蚩鸡饲养户由于管理不善,导致了一次意外事故的发生,造成了不该出现的经济损失。     

一起孔雀感染马立克氏病病例报道

正2015年9月份,大庆市大同区某林场饲养的孔雀发生了一起以肢体(腿、翅膀、脖颈)麻痹为主要症状的疫病,经临床观察、病理解剖、实验室诊断,最后确诊为慢性型马立克氏病,现将有关情况报道如下。1发病情况1.1临床症状大同区某林场饲养孔雀72只,其中母雀23只、公雀6只,幼雀43只(4~20周龄),2015年9月10日前后,该场所饲养的幼雀相继发病,主要表现为     

Practical information on classical fowl plague (highly pathogenic avian influenza)

This brief review summarises some structural and biological properties of the highly pathogenic avian influenza virus and its biological significance for animal and man. Sources of actual information in case of an acute disease outbreak are also given.     

高致病性禽流感(H5N1)的临床特征及控制措施

高致病性禽流感穴H５N１雪是由正粘病毒科流感病毒属病毒引起的急性接触性传染病，被国际兽疫局（OIE）定为一类传染病，可表现出临诊症状、呼吸道疾病、消化道症状、产蛋率下降、致死率高的急性出血性疾病。低致病性禽流感可表现为亚临诊症状，轻的呼吸道感染到无症状带毒（隐性感染）等多种流行形式。疾病严重程度取决于病毒毒株的毒力、被感染的禽种、有无并发症、禽群整体的体质及免疫力等。目前中国还未发现高致病性禽流感。根据流感病毒核蛋白（NP）和基质蛋白（MS）抗原性的不同，可将病毒分为A、B、C三个血清型，A型流感病毒…     

Comparison of electrocardiograms of chickens infected with viscerotropic velogenic Newcastle disease virus and virulent avian influenza virus

Electrocardiograms of chickens infected with viscerotropic velogenic Newcastle disease virus (NDV) or virulent avian influenza virus (AIV) were characterized and compared. The ECG were monitored by radiotelemetry and were recorded twice daily before virus infection and during the course of the infection. Thirteen lead II intervals, segments, and amplitudes were measured and analyzed. The ECG of NDV-infected chickens were characterized by lengthened (P less than or equal to 0.05) ST segments and increased (P less than or equal to 0.05) P amplitudes. The ECG of AIV-infected chickens were characterized by lengthened (P less than or equal to 0.05) RS intervals, ST segments, TP intervals, and PR segments and by increased (P less than or equal to 0.05) P amplitudes. The TP intervals and PR segments of ECG of AIV-infected chickens were significantly (P less than or equal to 0.05) longer than those of NDV-infected chickens. The pronounced conduction delays indicated in the ECG of AIV-infected chickens may have diagnostic importance.     

肉鸡禽流感低致病株H9N2的临床特征及控制

收稿日期：２００２－０１－０７肉鸡低致病性的禽流感的发生和流行是最近一二年的事，前几年在蛋鸡发病较为严重，而且以前的观点是肉鸡和后备鸡很少发病，现在看来肉鸡发病也呈上升趋势。养鸡户对肉鸡发生本病缺乏系统的了解，往往将本病和新城疫、传染性喉气管炎、传染性鼻炎、传染性支气管炎、大肠杆菌病、慢性呼吸道病等混淆。近来流行的禽流感血清亚型为H９N２，致病力低，一般不会引起高的死亡率，但若并发细菌病或其它病毒病时则会出现较高的死亡率。为了使肉鸡生产者加强对本病常识的认识，特将本病流行病学、临床症状、病理剖检…     

Iss from a virulent avian Escherichia coli

No single characteristic of virulent avian Escherichia coli has been identified that can be exploited in colibacillosis detection protocols. Research in our lab suggests a strong association between the presence of an iss DNA sequence with an isolate's disease-causing ability. The study presented here focuses on the techniques used in the expression, purification, and characterization of avian E. coli Iss protein. In brief, iss was cloned into an expression vector, the construct was transformed into a protease-deficient E. coli, and expression was induced. The protein was expressed as a glutathione-S-transferase (GST) fusion and purified by affinity chromatography. The GST portion was cleaved from Iss, Iss was harvested by affinity chromatography, and the identity of Iss was confirmed by N-terminal sequencing. Currently, purified Iss is being used to prepare hybridomas for production of monoclonal antibodies with the goal of evaluating anti-Iss as a reagent for the detection of virulent avian E. coli.     

Specific antibody responses and generation of antigenic variants in chickens immunized against a virulent avian influenza virus.

To examine the specificity of the antibody response to the influenza hemagglutinin and the generation of antigenic variants, chickens were immunized against the highly virulent H5 virus A/Ty/Ont/7732/66 (H5N9) and then challenged with a lethal dose of the virus. The antibody responses of these chickens to the hemagglutinin (HA) were examined with an enzyme-linked immunosorbent assay (ELISA) in which their sera were titrated for the ability to block the binding of monoclonal antibodies (MAbs) to five distinct neutralizing epitopes on the viral HA. Based on the ELISA results, a majority (5/6) of the chickens produced antibodies to three of the five neutralizing epitopes on the viral HA. After challenge, two of six immunized chickens shed virus and died; antigenic comparisons of isolates from these two chickens indicated the presence of an antigenic variant; i.e., there was a change in one neutralizing epitope on the HA of virus shed by one chicken. None of the chickens had produced antibodies to this particular epitope on the viral HA. Inoculation of chickens with this variant resulted in 100% mortality, demonstrating that a change in this particular epitope did not alter the virulence of the virus. These studies indicate that chickens immunized against highly virulent influenza viruses may excrete virulent variants following challenge with live virus.     

实时荧光定量RT-PCR方法检测禽流感病毒

根据禽流感病毒(Avian influenza virus,AIV)M基因上的保守序列,合成引物和荧光标记探针,以阳性AIVM基因质粒为标准品做标准曲线,建立了荧光定量逆转录聚合酶链反应(RRT-PCR)检测方法。结果表明,本试验建立的标准曲线循环阈值(Ct值)与模板浓度具有良好的线性关系,相关系数为0.999,灵敏度约为5拷贝/μL,相当于5个AIV颗粒,对新城疫病毒和其他禽病病毒无交叉反应,特异性好、重复性佳,为AIV检测提供了一种特异、敏感、快速的定量检测方法。对500份临床泄殖腔棉拭样品的检测,其结果阳性?阴性数与经典病毒分离方法符合率分别为91.2%?99.4%。在AIV临床样品筛检、流行病学监测等方面显示良好的应用前景。