Influence of applied nitrogen on potato part I: Yield,quality, and Nitrogen uptake

Fertilizer nitrogen (N) may be managed to increase crop production and profitability while reducing nitrate contamination of groundwater. A two-year field investigation was conducted to evaluate the effects of applied N on tuber yield and quality, dry matter production and N uptake of potato (Solanum tuberosum L. var. Russet Burbank) grown on irrigated sandy soils in Michigan. Nitrogen was applied as ammonium sulfate [(NH₄)₂SO₄] at rates of 0, 56 and 112, kg N ha^?1 in a single application at planting or 112 and 168 kg N ha^?1 in split applications during the growing season. Total tuber yield generally increased with N applications up to 112 kg N ha^?1. Only one of the three experimental sites showed an increase in marketable tuber yield when 112 kg N ha^?1 was split evenly between planting and tuber initiation. Tuber specific gravity was not affected by N rate. Nitrogen rates of 112–168 kg N ha^?1 maximized dry matter production and plant tissue N concentration at onset of maturity and harvest. Tuber N concentration at harvest ranged from 13–17 g kg^?1 at two of the three locations. Values for the third experiment were 10–13 g N kg^?1. Whole crop N uptake at onset of senescence ranged from 45 to 225 kg N ha^?1 across all locations and treatments. An average of 67 percent of this N was found in tubers at harvest. Nitrogen fertilization exceeded N removal in harvested tubers by more than 50 kg N ha^?1 only for the 168 kg N ha^?1 treatment. These results indicate that acceptable tuber yield can be obtained with lower N rates than those currently used by most producers, with the potential for reducing net loss of N from the soil.     

Orchardgrass response to different types,rates and application patterns of dairy manure

Manure management is a difficult task on many intensive dairy farms. Crops that can utilize large quantities of manure N, yield quality forage with larger rates of manure application, and allow manure spreading at different times in a year can simplify that task. A study was conducted in 1990 and 1991 on a Copake sandy loam soil (mixed mesic) in New Milford, Connecticut. The objectives were: (1) to measure and compare dry matter (DM) response of orchardgrass (Dactylis glomerata L.) to different amounts and application times of N fertilizer and liquid and solid cattle manure; and (2) to determine crop uptake of fertilizer and manure N. Fertilizer and liquid and solid manure were applied to the soil surface annually in amounts of 150, 300 or 450 kg N ha⁻¹ in one, two or four equal applications. Orchardgrass dry matter production increased over the entire range of N amounts from all sources. Yields varied from approximately 2500 kg DM ha⁻¹ for control plots (0 kg N) to 10600 kg for plots receiving 450 kg N ha⁻¹ either as fertilizer or liquid manure. Crop response to liquid manure application was greater in year one with abundant rainfall than in year two with dry conditions during most of the growing season, whereas crop response to solid manure application improved in the second year, due to the availability of residual organic N. Orchardgrass was more sensitive to the timing of fertilizer N application than to manure N application. Despite the large differences in weather patterns experienced during this study, analysis of application patterns indicated that manure could be applied throughout the growing season to crop stubble (post-harvest) with comparable rates of uptake overall. N uptake in control plots averaged 56 kg N ha⁻¹ for both years, compared to 340 kg N ha ⁻¹ for fertilizer plots, 250 kg N ha ⁻¹ for liquid manure plots and 190 kg N ha⁻¹ for solid manure plots receiving 450 kg total N ha ⁻¹.     

Effect of fertilizer nitrogen source and cattle slurry on herbage production and nitrogen utilization

A field plot experiment was carried out on an established grassland sward from 1983–88 inclusive to examine the effects of time of application, chemical form of nitrogen (N) and cattle slurry dry matter (DM) content on yield and efficiency of N use. Four forms of fertilizer N (a semi-organic fertilizer, a combined 2.1:1 (w/w) semi-organic/calcium ammonium nitrate (CAN) fertilizer, CAN and urea, each supplying 300 kg N ha^?1 year^?1, were applied with or without unseparated or separated cattle slurry at 93 and 73 g kg^?1 DM respectively, both supplying approximately 150 kg N ha^?1 year^?1. All fertilizers and slurries were applied in three equal dressings (February/March, May/June and July/August). The efficiency of use of fertilizer and slurry N was evaluated by measuring DM yield, N uptake and apparent recovery of N in herbage at all harvests during each growing season. Fertilizer N application significantly increased (P<0.001) the mean yields of herbage at each harvest in all years. The form of fertilizer N had no significant effect (P>0.05) on first harvest and total herbage yields, nor on N uptakes by herbage at the first harvest in any year. The performance of urea and of CAN was more variable at the second and third harvests relative to that of the semi-organic or combined 2.2:1 (w/w) semi-organic/CAN sources which had similar efficiencies of N use. Lower DM production was associated with reduced uptake of N. Values for mean overall apparent recovery of N ranged from 57.9 ± 2.67% for the semi-organic fertilizer to 50.2±3.05% for CAN. Unseparated cattle slurry and separated cattle slurry produced similar herbage yields and N responses that were lower and more variable than with fertilizer N. The overall mean apparent recovery of N from unseparated cattle slurry was 25.5 ± 5.03% compared to 5.0 ± 4.74% for separated cattle slurry. Efficiency of N use was highest with spring applications and least with mid-season applications. Recoveries ranged from ?29% for separated cattle slurry applied in June 1984 to 56% for unseparated and separated cattle slurry applied in February 1988 and June 1987 respectively. No interactions were recorded between cattle slurry and fertilizer N in terms of DM production or N uptake by herbage. The results of this study support the use of a fertilizer N source, selected on a least-cost basis, in combination with slurry to promote spring herbage production. For subsequent production, N should be supplied in fertilizer form only. The use of urea is risky under low rainfall conditions. Mechanical separation did not improve the efficiency of use of slurry N.     

The response of manured forage maize to starter phosphorus fertilizer on chalkland soils in southern England

The impact of various starter phosphorus (P) fertilizers on the growth, nutrient uptake and dry‐matter (DM) yield of forage maize (Zea mais) continuously cropped on the same area and receiving annual, pre‐sowing, broadcast dressings of liquid and semi‐solid dairy manures was investigated in two replicated plot experiments and in whole‐field comparisons in the UK. In Experiment 1 on a shallow calcareous soil (27 mg l^?1 Olsen‐extractable P) in 1996, placement of starter P fertilizer (17 or 32 kg ha^?1) did not benefit crop growth or significantly (P > 0·05) increase DM yield at harvest. However, in Experiment 2 on a deeper non‐calcareous soil (41 mg l^?1 Olsen‐extractable P) in 1997, placement of starter P fertilizer (19 or 41 kg P ha^?1), either applied alone or in combination with starter N fertilizer (10 or 25 kg N ha^?1), significantly increased early crop growth (P < 0·01) and DM yield at harvest by 1·3 t ha^?1 (P < 0·05) compared with a control without starter N or P fertilizer. Placement of starter N fertilizer alone did not benefit early crop growth, but gave similar yields as P, or N and P, fertilizer treatments at harvest. Large treatment differences in N and P uptake by mid‐August had disappeared by harvest. In field comparisons over the 4‐year period 1994–97, the addition of starter P fertilizer increased field cumulative surplus P by over 70%, but without significantly (P > 0·05) increasing DM yield, or nutrient (N and P) uptake, compared with fields that did not receive starter P fertilizer. The results emphasized the extremely low efficiency with which starter P fertilizers are utilized by forage maize and the need to budget manure and fertilizer P inputs more precisely in order to avoid excessive soil P accumulation and the consequent increased risk of P transfer to water causing eutrophication.     

Plant and soil responses to nitrogen and phosphorus fertilization of bromegrass‐dominated haylands in Saskatchewan,Canada

Field experiments were conducted at three different sites in Saskatchewan, Canada (Colonsay, Vanscoy and Rosthern) over two years (2005 and 2006) to determine the effects of dribble‐banded and coulter‐injected liquid fertilizer applied in the spring of 2005 at 56, 112 and 224 kg N ha^?1 with and without P at 28 kg P₂O₅ ha^?1. The three sites were unfertilized, 7‐ to 8‐year old stands of mainly meadow bromegrass (Bromus riparius)‐dominated haylands. All fertilization treatments produced significantly (P ≤ 0·05) higher dry matter yield than the control in the year of application at the three Saskatchewan sites. There was no significant difference between the two application methods (surface dribble band vs. coulter injected) for any fertilizer treatments. The addition of 28 kg P₂O₅ ha^?1 P fertilizer along with the N fertilizer did not have a significant effect on yield in most cases. In the year of application, increasing N rates above 56 kg N ha^?1 did not significantly increase yield over the 56 kg N ha^?1 rate in most cases, but did increase N concentration, N uptake and protein concentration. A significant residual effect was found in the high N‐rate treatments in 2006, with significantly higher yield and N uptake. In 2005, the forage N and P uptake in the fertilized treatments were significantly higher than the control in all cases. The N uptake at the three Saskatchewan sites increased with increasing N rate up to the high rate of 224 kg N ha^?1, although the percent recovery of applied N decreased with increasing rate. The P fertilization with 28 kg P₂O₅ ha^?1 also increased P uptake. Overall, rates of fertilizer of approximately 56 kg N ha^?1 appear to be sufficient to produce nearly maximum forage yield and protein concentration of the grass in the year of application.     

Recovery of starter nitrogen-15 fertilizer with supplementarily applied ammonium nitrate on irrigated potato

Nitrogen contamination in ground water of potato (Solanum tuberosum L.) producing areas has indicated a need for improved management of N and water, particularly on sandy soil. Therefore, a field experiment was conducted with the objective of following the recovery and partitioning of starter¹⁵NH₄ and¹⁵NO₃ into potato plant tops and tubers in conjunction with additional supplementarily applied NH₄NO₄. Potato plants treated with starter¹⁵NH₄ or¹⁵NO₃ tended to increase the percent recovery of starter¹⁵N in tubers sampled from one growth time to the next to reach nearly 40% recovery toward the end of the season. Whole plants reached peak recovery of around 50% of the starter¹⁵N near mid-season. From then on, there was a trend for loss of starter¹⁵N by senescence, defoliation or translocation to the roots. The percent recovery of starter¹⁵N was significantly higher at final tuber harvest (not whole plants) for the treatment with starter¹⁵NH₄ at 112 kg ha^?1 combined with 112 kg ha^?1 of supplemental N as compared to the treatment with 112 kg ha^?1 of starter¹⁵NH₄ plus 224 kg ha^?1 of supplemental N. This difference may have been a result of isotope dilution. Early in June the accumulation of starter¹⁵NO₃ in whole plants was about five times as high as that from starter¹⁵NH₄. Later there was no difference in percent recovery of these two forms of N. The temporary delay in starter¹⁵NH₄ uptake was probably related to slow nitrification early in the season instead of preferential uptake of starter¹⁵NO₃.     

Interactions between non-flooded mulching cultivation and varying nitrogen inputs in rice–wheat rotations

A 3-year field experiment examined the effects of non-flooded mulching cultivation and traditional flooding and four fertilizer N application rates (0, 75, 150 and 225 kg ha⁻¹ for rice and 0, 60,120, and 180 kg N ha⁻¹ for wheat) on grain yield, N uptake, residual soil N_min and the net N balance in a rice–wheat rotation on Chengdu flood plain, southwest China. There were significant grain yield responses to N fertilizer. Nitrogen applications of >150 kg ha⁻¹ for rice and >120 kg ha⁻¹ for wheat gave no increase in crop yield but increased crop N uptake and N balance surplus in both water regimes. Average rice grain yield increased by 14% with plastic film mulching and decreased by 16% with wheat straw mulching at lower N inputs compared with traditional flooding. Rice grain yields under SM were comparable to those under PM and TF at higher N inputs. Plastic film mulching of preceding rice did not affect the yield of succeeding wheat but straw mulching had a residual effect on succeeding wheat. As a result, there was 17–18% higher wheat yield under N0 in SM than those in PM and TF. Combined rice and wheat grain yields under plastic mulching was similar to that of flooding and higher than that of straw mulching across N treatments. Soil mineral N (top 60 cm) after the rice harvest ranged from 50 to 65 kg ha⁻¹ and was unaffected by non-flooded mulching cultivation and N rate. After the wheat harvest, soil N_min ranged from 66 to 88 kg N ha⁻¹ and increased with increasing fertilizer N rate. High N inputs led to a positive N balance (160–621 kg ha⁻¹), but low N inputs resulted in a negative balance (−85 to −360 kg ha⁻¹). Across N treatments, the net N balances of SM were highest among the three cultivations systems, resulting from additional applied wheat straw (79 kg ha⁻¹) as mulching materials. There was not clear trend found in net N balance between PM and TF. Results from this study indicate non-flooded mulching cultivation may be utilized as an alternative option for saving water, using efficiently straw and maintaining or improving crop yield in rice–wheat rotation systems. There is the need to evaluate the long-term environmental risks of non-flooded mulching cultivation and improve system productivity (especially with straw mulching) by integrated resource management.     

Response of russet norkotah clonal selections to nitrogen fertilization

The low vine vigor and high N requirement of Russet Norkotah may lead to N loss and groundwater contamination on coarse-textured soils. Recent clonal selections from Texas have produced strains that have larger and stronger vines, which may alter N requirements. This twoyear study examined the N use efficiency (NUE), yield, and quality of Russet Norkotah strain selections fertilized with different N levels on a Hubbard loamy sand in central Minnesota. The selections, Texas Norkotah Strain (TXNS) 112, TXNS 223, and TXNS 278 were grown with standard Russet Norkotah under irrigated conditions and received total N levels of 28, 112, 224, or 336 kg ha^-1. Total, marketable, and large (>340 g) tuber yields increased linearly (P>0.05) with rate of N application in 1998 but not in 1997. The genotype main effect was not significant for any of the tuber yield parameters measured based on fresh weight. Vine, tuber, and total dry biomass yields were 116%, 5.8%, and 13.2%, respectively, higher with the selections than Russet Norkotah. Harvest index (HI), or the proportion of total dry matter partitioned to tubers, was 7% greater for Russet Norkotah than the TXNS selections, reflecting the larger vine growth of the selections. The selections accumulated significantly higher N in the vines (0.113 kg kg^-1 N) than the standard clone (0.053 kg kg^-1 N) as N rate increased from 28 to 336 kg ha^-1, and the difference between the selections and the standard clone was larger at higher N rates than at lower N rates. Russet Norkotah partitioned 10% more N to tubers than did the TXNS selections, reflecting the difference in HI between the standard cultivar and its clones. Nitrogen recovered from fertilizer N applied in addition to the 28 kg ha^-1 starter N (NUE₂₈) averaged 36% and varied little with genotype, N rate, or cropping year. Biomass accumulation from similar N additions (AUE), however, was significantly higher for the selections than Russet Norkotah at 112 kg N ha^-1 in 1997 only. At low N rate (112 kg ha^-1), the selections had higher physiological use efficiency (PUE₂₈) (mean 45.9 g g¹) than Russet Norkotah (25.9 g g¹). Results from this study demonstrate that, although N recovery was similar for the four genotypes, the Texas Norkotah strains produced greater biomass than Russet Norkotah per kg N applied at low rate in 1997 and per kg of fertilizer N absorbed by the plant in both years. However, under the conditions of this study, higher biomass of the selections did not translate into a marketable yield advantage over the standard cultivar.     

Nitrogen efficiency in wheat under rainfed Mediterranean conditions as affected by split nitrogen application

A 3-year field experiment in rainfed Vertisol was designed to study the effects of timing and splitting of N fertilizer on the efficiency of nitrogen in wheat (Triticum aestivum L.). A single rate of 150 kg N ha⁻¹ was used, different fractions being applied at sowing, tillering and stem elongation. The experiment was designed as a randomized complete block with four blocks. At the same time, a ¹⁵N experiment was conducted within the main experiment area, with microplots, to quantify N uptake from fertilizer and soil. Mean wheat use of N fertilizer ranged from 14.1% when applied at sowing to 54.8% when applied as a top dressing at the beginning of stem elongation. The mean annual contribution of soil residual N and mineralization was 152 kg N ha⁻¹, representing a considerable proportion of total wheat N uptake, ranging from 83.2% when N fertilizer was applied in the fall to 49.4% when it was applied at stem elongation. This would account for the poor and inconsistent response of grain yield and N efficiency indices, and for the importance of soil N in Vertisols for predicting wheat N fertilizer requirements, due to the carryover effect. Application of N fertilizer to wheat preferably as a top dressing, between tillering and stem elongation, is a strategy to be recommended from the standpoint both of the environment and of farmer returns.     

Influence of mineral nitrogen on nitrogen fixation by lupin (Lupinus angustifolius) as assessed by 15N isotope dilution methods

The effect of soil mineral N (NO₃+NH₄) on the proportion (P) of N₂ fixed by lupin (Lupinus angustifolius cv. Illyarrie) grown on red-earth over two seasons was measured in field crops by ¹⁵N isotope dilution, using either the natural abundance of ¹⁵N or enrichment of soil N with K¹⁵NO₃. The reference plants used were wheat or linseed. Differences in concentrations of soil mineral N were induced by pretreatment with either cereal straw (3, 6 or 15 t ha⁻¹) or ammonium nitrate (60, 90, 100, 120, or 150 kg N ha⁻¹).The two ¹⁵N method produced similar estimates of P in untreated soil, but for some treatments there were substantial discrepancies between the natural abundance and the enrichment methods. In addition, although the effect of fertilizer N was evident regardless of the reference plant, linseed gave higher estimates of P than did wheat. The possible reasons for these differences are discussed.From values determined after enrichment of soil N with ¹⁵N and with wheat as a reference, P declined by up to 0.23 units with additions of ammonium nitrate up to 150 kg N ha⁻¹. In the N fertilizer treatments, mineral N substituted for fixed N₂ since the total N of lupin was unaffected by the level of soil mineral N. Pretreatment with N fertilizer (90 or 150 kg N ha⁻¹) diminished the potential benefit to soil N from N₂ fixation by lupin, producing an average net loss of 33 kg N ha⁻¹. At sowing, cereal straw had reduced soil mineral N. However, although this soil treatment did not significantly reduced total lupin N, no significant increase in N₂ fixation was detected.     

Residual effect of poultry litter applied to cotton in conservation tillage systems on succeeding rye and corn

The burgeoning poultry industry in the southeastern US is presenting a major environmental problem of safe disposal of poultry litter (PL). In a comprehensive study, we explored ways of PL use in conservation tillage-based cotton (Gossypium hirsutum L.) production systems on a Decatur silt loam soil in north Alabama, from 1996 to 1999. The study reported here-in presents the residual effects of PL applied to cotton in mulch-till (MT) and no-till (NT) conservation tillage systems in 1997 and 1998 cropping seasons on N uptake, growth, and yield of rye (Secale cereale, L.) cover crop and rotational corn (Zea mays L.) in 1999. Rye was grown without additional N, whereas corn was grown at three inorganic N levels (0, 100, and 200 kg N ha⁻¹). Poultry litter was applied to cotton in 1997 and 1998 at 0, 100, and 200 kg N ha⁻¹. Residual N from PL applied to cotton in 1997 and 1998 produced up to 2.0 and 17.3 Mg ha⁻¹, respectively, of rye cover crop and corn biomass (includes 7.1 Mg ha⁻¹ of corn grain yield) without additional fertilizer. Therefore, in addition to supplying crop residues which reduce soil erosion, increase soil organic matter, and conserve soil moisture, the rye cover crop was able to scavenge residual N left by the cotton crop, which would otherwise, be at risk of being leached and pollute groundwater resources. Poultry litter applied to cotton also increased corn grain quality as shown by up to 100% increase in grain N content compared to the 0N treatment. Using PL with a slower rate of N release compared to inorganic fertilizer to meet some of the N requirements of corn, will not only reduce N fertilizer costs for corn, but will also reduce the risk of nitrate N leaching into groundwater. The maximum amount of crop residues added to the cotton based cropping system by residual N from PL and inorganic N was 21.3 Mg ha⁻¹. This will lead to an increase in soil organic carbon and soil structure in the long term and a reduction in soil erosion, thereby further improving soil productivity, while at the same time, protecting the environment from nitrate pollution and soil degradation. Our study demonstrates that cotton under conservation tillage system in combination with rye cover crop and rotational corn cropping could use large quantities of PL thereby avoiding serious potential environmental hazards.     

Rice straw mulching and nitrogen response of no-till wheat following rice in Bangladesh

Wheat (Triticum aestivum L.) cultivation in no-till soil of a postrice harvest field utilizes residual soil moisture and reduces the time period from rice harvest to wheat seeding in intensive rice-wheat cropping systems. Some of the major constraints in no-till wheat production are high weed infestation, poor stand establishment due to rapid drying of topsoil and low nitrogen use efficiency (NUE). A field experiment was conducted at the research farm of the Wheat Research Centre, Dinajpur, Bangladesh, for two consecutive years to overcome those constraints, to evaluate rice straw as mulch, and to determine the optimum application rate of nitrogen (N) for no-till wheat. The treatments included 12 factorial combinations of three levels of mulching: no mulch (M₀), surface application of rice straw mulch at 4.0 Mg ha⁻¹ that was withdrawn at 20 days after sowing (M₁), the same level of mulch as M₁ but allowed to be retained on the soil surface (M₂), and four nitrogen levels (control 80, 120 and 160 kg ha⁻¹). Rice straw mulching had a significant effect on conserving initial soil moisture and reducing weed growth. Root length density and root weight density of wheat were positively influenced both by straw mulching and N levels. N uptake and apparent nitrogen recovery of applied N fertilizer were higher in mulch treatments M₁ and M₂ as compared to M₀. Also mulch treatment of M₁ and M₂ were equally effective at conserving soil moisture, suppressing growth of weed flora, promoting root development and thereby improved grain yield of no-till wheat. N application of 120 kg ha⁻¹ with straw mulch was found to be suitable for no-till wheat in experimental field condition.     

Effect of nitrogen fertilization and rice stubble management techniques on soil moisture content,soil nitrogen status,and nitrogen uptake by wheat

The effects of nitrogen fertilization and stubble treatment on soil mineral-N content, moisture status and subsequent crop N uptake were studied in a series of wheat (Triticum aestivum L.) crops planted soon after rice (Oryza sativa L.) harvest. The experiments were part of a programme to define optimum management systems for intensive rice/upland cereal rotations. Stubble management, N application time and rate all influenced amount of soil mineral-N, accounting for up to 52, 67 and 75%, respectively, of the mineral-N variation. In turn, variation in soil mineral-N explained up to 70% of the variation in N accumulation by wheat. N accumulation was highly correlated with crop yield (r² up to 0.95).Incorporating large quantities of rice stubble at wheat sowing reduced soil NO-₃-N concentration by 36% at stem elongation. Both N uptake and yield were reduced by 38% on these plots. Increasing quantities of rice stubble retained on the soil surface increased soil NO₃-N concentration by 46%, and wheat on these plots had a 29% increase in N uptake and a 37% increase in yield. Stubble burning rather than retention on the surface resulted in lower soil NO₃-N concentration, and this was ascribed to ammonia volatilization resulting from fertilizer contact with ash, and to reduced mineralisation in the drier soil.Application of N at wheat sowing significantly increased mineral-N status at least until stem elongation, while fertilization at tillering or stem elongation significantly increased soil mineral-N content at least until anthesis.It was concluded that stubble and fertilization management techniques can be manipulated in order to regulate soil mineral-N status, which in turn determined plant N uptake. Plant N uptake determined yield.     

Effect of fertilization on crop responses and solute transport for rice crop in a sub-humid and sub-tropical region

In order to increase the efficacy of water and control the losses of fertilizer, it is necessary to assess the influence of level of fertilization on crop responses, movement and balance of water and solutes from fertilizers in the root zone. With this goal, the reported study was undertaken to determine the effect of fertilization on crop responses and fertilizer solute transport in rice crop field in a sub-humid and sub-tropical region. Field experiment was conducted on rice crop (cultivar IR 36) during the years 2003, 2004, and 2005. The experiment included four fertilizer treatments comprising different levels of fertilizer application. The fertilizer treatments during the experiment were: F₁ = control with N:P₂O₅:K₂O as 0:0:0 kg ha^?1; F₂ = fertilizer application of N:P₂O₅:K₂O as 80:40:40 kg ha^?1; F₃ = fertilizer application of N:P₂O₅:K₂O as 120:60:60 kg ha^?1 and F₄ = fertilizer application of N:P₂O₅:K₂O as 160:80:80 kg ha^?1. The results of the investigation revealed that the magnitudes of crop parameters such as grain yield, straw yield, and maximum leaf area index increased with increase in fertilizer application rate. The levels of fertilization had very little effect on water loss via deep percolation and water use by the crop. The levels of fertilization had considerable effect on N leaching loss and uptake of N whereas it had no significant impact on leaching loss of water-soluble phosphorus. This indicated that PO₄-P leaching loss was very low in the soil solution as compared to nitrogen due to fixation of phosphorus in soils. The results also revealed that increase in level of fertilization increased water use efficiency considerably by increased crop yield. From the observed data of nutrient use efficiency, crop yield and environmental pollution, the fertilization rate of N:P₂O₅:K₂O as 80:40:40 kg ha^?1 (F₂) was the most suitable fertilizer treatment for rice crop among studied treatments.     

Herbage and nitrogen yields,fixation and transfer by white clover to companion grasses in grazed swards under different rates of nitrogen fertilization

In grass–legume swards, biologically fixed nitrogen (N) from the legume can support the N requirements of the grass, but legume N fixation is suppressed by additional fertilizer N application. This study sought to identify a fertilizer N application rate that maximizes herbage and N yields, N fixation and apparent N transfer from white clover to companion grasses under intensive grazing at a site with high soil‐N status. During a 3‐year period (2011–2013), swards of perennial ryegrass and of perennial ryegrass–white clover, receiving up to 240 kg N ha^?1 year^?1, were compared using isotope dilution and N‐difference methods. The presence of white clover increased herbage and N yields by 12–44% and 26–72%, respectively. Applications of N fertilizer reduced sward white clover content, but the effect was less at below 120 kg N ha^?1. The proportion of N derived from the atmospheric N fixation was 25–70%. Nitrogen fixation ranged from 25 to 142 kg N ha^?1 measured using the isotope dilution method in 2012 and from 52 to 291 kg N ha^?1 using the N‐difference method across all years. Fertilizer N application reduced the percentage and yield of fixed N. Transfer of N from white clover to grass was not confirmed, but there was an increased N content in grass and soil‐N levels. Under intensive grazing, the maximum applied N rate that optimized herbage and N yields with minimal effect on white clover content and fixation rates was 60–120 kg N ha^?1.     

Identification of traits to improve the nitrogen-use efficiency of wheat genotypes

Nitrogen (N) fertilizer represents a significant cost for the grower and may also have environmental impacts through nitrate leaching and N₂O (a greenhouse gas) emissions associated with denitrification. The objectives of this study were to analyze the genetic variability in N-use efficiency (grain dry matter (DM) yield per unit N available from soil and fertilizer; NUE) in winter wheat and identify traits for improved NUE for application in breeding. Fourteen UK and French cultivars and two French advanced breeding lines were tested in a 2 year/four site network comprising different locations in France and in the UK. Detailed growth analysis was conducted at anthesis and harvest in experiments including DM and N partitioning. Senescence of either the flag leaf or the whole leaf canopy was assessed from a visual score every 3-4 days from anthesis to complete canopy senescence. The senescence score was fitted against thermal time using a five parameters monomolecular-logistic equation allowing the estimation of the timing of the onset and the rate of post-anthesis senescence. In each experiment, grain yield was reduced under low N (LN), with an average reduction of 2.2 t ha⁻¹ (29%). Significant N × genotype level interaction was observed for NUE. Crop N uptake at harvest on average was reduced from 227 kg N ha⁻¹ under high N (HN) to 109 kg N ha⁻¹ under LN conditions while N-utilization efficiency (grain DM yield per unit crop N uptake at harvest; NUtE) increased from 34.0 to 52.1 kg DM kg⁻¹ N. Overall genetic variability in NUE under LN related mainly to differences in NUtE rather than N-uptake efficiency (crop N uptake at harvest per unit N available from soil and fertilizer; NUpE). However, at one site there was also a positive correlation between NUpE and NUE at LN in both years. Moreover, across the 2 year/four site network, the N × genotype effect for NUpE partly explained the N × genotype effect for grain yield and NUE. Averaging across the 16 genotypes, the timing of onset of senescence explained 86% of the variation in NUtE amongst site-season-N treatment combinations. The linear regression of onset of senescence on NutE amongst genoytpes was not significant under HN, but at three of the four sites was significant under LN explaining 32-70% of the phenotypic variation amongst genotypes in NutE. Onset of senescence amongst genotypes was negatively correlated with the efficiency with which above-ground N at anthesis was remobilized to the grain under LN. It is concluded that delaying the onset of post-anthesis senescence may be an important trait for increasing grain yield of wheat grown under low N supply.     

Biomass production and nitrogen accumulation and remobilisation by Miscanthus × giganteus as influenced by nitrogen stocks in belowground organs

The nitrogen (N) requirement of dedicated crops for bioenergy production is a particularly significant issue, since N fertilisers are energy-intensive to make and have environmental impacts on the local level (NO₃ leaching) and global level (N₂O gas emissions). Nitrogen nutrition of Miscanthus × giganteus aboveground organs is assumed to be dependent on N stocks in belowground organs, but the precise quantities involved are unknown. A kinetic study was carried out on the effect of harvest date (early harvest in October or late harvest in February) and nitrogen fertilisation (0 or 120 kg N ha⁻¹) on aboveground and belowground biomass production and N accumulation in established crops. Apparent N fluxes within the crop and their variability were also studied.Aboveground biomass varied between 24 and 28 t DM ha⁻¹ in early harvest treatments, and between 19 and 21 t DM ha⁻¹ in late harvest treatments. Nitrogen fertilisation had no effect on crop yield in late harvest treatments, but enhanced crop yield in early harvest treatments due to lower belowground biomass nitrogen content. Spring remobilisation, i.e. nitrogen flux from belowground to aboveground biomass, varied between 36 and 175 kg N ha⁻¹, due to the variability of initial belowground nitrogen stocks in the different treatments. Autumn remobilisation, i.e. nitrogen flux from aboveground to belowground organs, varied between 107 and 145 kg N ha⁻¹ in late harvest treatments, and between 39 and 93 kg N ha⁻¹ in early harvest treatments. Autumn remobilisation for a given harvest date was linked to aboveground nitrogen accumulation in the different treatments. Nitrogen accumulation in aboveground biomass was shown to be dependent firstly on initial belowground biomass nitrogen stocks and secondly on nitrogen uptake by the whole crop.The study demonstrated the key role of belowground nitrogen stocks on aboveground biomass nitrogen requirements. Early harvest depletes belowground nitrogen stocks and thus increases the need for nitrogen fertiliser.     

Accounting for residual effects of previously applied nitrogen fertilizer on intensively managed grasslands

Only 0·20–0·70 of the fertilizer-nitrogen (N) applied to grassland is taken up in herbage in the harvest directly following application. Residual effects at subsequent harvests can be large but are poorly quantified, and rarely taken into account in current management practices. An increased understanding of N-use efficiency per harvest can improve operational management. This study systematically assessed the residual effects of previously applied N fertilizer on N uptake, dry matter (DM) yield and soil mineral-N (SMN) during the whole of the growing season. It is based on field experiments conducted on peat and mineral soils in 1991–1994. Statistical models were derived for SMN, N uptake and DM yield as a function of previously and freshly applied N fertilizer. There were clear residual effects of previously applied N in later cuts. They were relatively greater at higher levels of N fertilizer. On peat soils, 0·15–0·25 of the N applied was recovered as SMN. On mineral soils the proportion was maximally 0·08. There was a clear relationship between SMN and N uptake in the subsequent cut on mineral soils but not on peat soils. The value of SMN as a tool to adjust fertilizer-N application rates was hence found to be limited. There were clear relationships between the amount of previously applied N and the N uptake in subsequent cuts, on both soil types and over the whole of the growing season. It was concluded that the total amount of previously applied N is a useful indicator for adjusting N-fertilizer application rates.     

Effect of seedpiece spacing and nitrogen fertilization on tuber yield, yield components, and nitrogen use efficiency parameters of two potato cultivars

The effect of seedpiece spacing on the efficiency of nitrogen (N) use by the potato crop is generally unknown. The objective of this experiment was to determine the effect of seedpiece spacing on tuber yield, yield components and N use efficiency parameters of two potato cultivars. Potato cultivars Atlantic and Shepody were grown at two rates of N fertilization (0 or 100 kg N ha^?1) and three seedpiece spacings (20, 30, or 40 cm) in 2000 to 2002. Wider seedpiece spacing increased mean tuber weight and the number of tubers per stem, but decreased total tuber yield. The higher tuber yield at the narrow seedpiece spacing was attributed to higher biomass production in combination with lower tuber specific gravity. Seedpiece spacing had no consistent effect on plant N accumulation, and therefore no consistent effect on N uptake efficiency (plant N accumulation /N supply from the soil plus fertilizer). However, a small increase in soil NO₃-N concentration in the hill at topkill at wider seedpiece spacing suggested plant N accumulation was slightly reduced at wider seedpiece spacing, but at a level that could not be detected from a plant-based measure of N accumulation. The reduced dry matter accumulation, but similar plant N accumulation, resulted in lower N use efficiency (plant dry matter accumulation / N supply) at wider seedpiece spacing. Wider seedpiece spacing also resulted in generally lower values of N utilization efficiency (plant dry matter accumulation / plant N accumulation) for the 40-cm compared with the 20- and 30-cm seedpiece spacings. Effects of seedpiece spacing on N use efficiency parameters were generally consistent across cultivars and fertilizer N rates. Wider seedpiece spacing did reduce the efficiency of N use by the potato crop; however, the magnitude of the effect was small under the conditions of this study.     

Effects of N application on agronomic and environmental parameters in silage maize production on sandy soils

The current nitrogen (N) use in silage maize production can lead to considerable N losses to the environment. Maize growers fear that a reduction of N inputs needed to minimize N losses might depress yields. The objective of this study was therefore to quantify: (1) the response of silage maize dry matter (DM) yields to N, (2) the economically optimal N reserve, and (3) the trade-off between silage maize DM yield and N losses. The indicators of N losses used in this study were the difference between N input and N uptake and the post-harvest residual soil mineral N. Regression models were used to fit DM yields and N uptakes of silage maize measured in 25 experiments on sandy soils in the Netherlands to the sum (SUMN) of the soil mineral N reserve (SMN_early) in March–April, plus mineral N in fertilizer, plus ammonium N in spring-applied slurry. The values obtained for the economically optimal SUMN in the upper 30 and 60 cm of soil were, respectively, 173 and 195 kg N ha⁻¹, when we assumed that the value of 1 kg fertilizer N equals the value of 5 kg silage DM. The economically optimal SUMN was not significantly related to the attainable DM yield. The apparent N recovery (ANR) of maize averaged 53% at the economically optimal SUMN. The ANR rose considerably, however, when N was applied at lower rates, indicating that N losses may be considerably smaller in less intensive maize cropping. When maize was fertilized at 100 kg N ha⁻¹ below the economic optimum, the ANR was 73%, the difference between the mineral N input and the N crop uptake decreased by 57 kg N ha⁻¹ and the soil mineral N residue at the end of the growing season (0–60 cm) decreased by 24 kg N ha⁻¹. The associated reduction in DM yield averaged 16%. Fertilizer prices would have to be as much as four times higher to make maize growers spontaneously reduce the application rates by a 100 kg N ha⁻¹, however. It is concluded that adjusting the N input to a level below the economically optimal rate can reduce the risks for N losses to the environment associated with conventional maize production, with a limited effect on silage yields.