Microbial activities in a histosol: Effects of wood ash and NPK fertilizers

Mineralization of organic matter and microbial activities in an intensively cultivated acid, N-rich peat soil planted with Salix sp. cv. aquatica were examined for 3 yr. The soil was amended with wood ash or NPK fertilizers providing N as ammonium nitrate or urea. The wood ash amendment (10 tons ha^?1) increased soil pH from 4.6 to 5.5 and increased markedly all microbial activities measured, resulting in increased mineralization and N availability, and in loss of 9% total soil N during the first year. The addition of ammonium nitrate caused a corresponding though less pronounced increase in N mineralization. Cellulose decomposition increased in all amended soils, reaching rates 53–86% higher than in non-amended soil. Potential N₂ fixation (C₂H₂ reduction) by free-living organisms was increased by the ash-amendment. Potential denitrification rates were positively correlated (r = 0.98) with the presence of water-soluble organic-C, which was more abundant in ash-amended and non-amended soils than in the soils fertilized with N.     

Application of wood ash accelerates soil respiration and tree growth on drained peatland

Forested peatlands contain large pools of terrestrial carbon. As well as drainage, forest management such as fertilizer application can affect these pools. We studied the effect of wood ash (application rates 0, 5 and 15 t ha^?1) on the heterotrophic soil respiration (CO₂ efflux), cellulose decomposition, soil nutrients, biomass production and amount of C accumulated in a tree stand on a pine‐dominated drained mire in central Finland. The ash was spread 13 years before the respiration measurements. The annual CO₂ efflux was statistically modelled using soil temperature as the driving variable. Wood ash application increased the amounts of mineral nutrients of peat substantially and increased soil pH in the uppermost 10 cm layer by 1.5–2 pH units. In the surface peat, the decomposition rate of cellulose in the ash plots was roughly double that in control plots. Annual CO₂ efflux was least on the unfertilized site, 238 g CO₂‐C m^?2 year^?1. The use of wood ash nearly doubled CO₂ efflux to 420–475 g CO₂‐Cm^?2 year^?1 on plots fertilized with 5–15 t ha^?1 of ash, respectively. Furthermore, ash treatments resulted also in increased stand growth, and during the measurement year, the growing stand on ash plots accumulated carbon 11–12 times faster than the control plot. The difference between peat C emission and amount of C sequestered by trees on the ash plots was 43–58 g C m^?2, while on the control plot it was 204 g C m^?2. Our conclusion is that adding wood ash as a fertilizer increases more C sequestration in the tree stand than C efflux from the peat.     

Liming and sulfur amendments improve growth and yields of maize in Rubona Ultisol and Nyamifumba Oxisol

Aluminum toxicity is a major limitation to crop production on highly weathered and leached soils in Rwanda. Moreover, sulfur though widely deficient in Rwanda acidic soils has received little attention by soil fertility researchers. A field experiment on maize response and soil nutrients status to liming materials of travertines at 3.4 t ha^?1, ash wood 1.2 t ha^?1 of CaO equivalent and sulfur at 10 kg ha^?1 combined with NPK at 80, 60, and 45 kg ha^?1 respectively was conducted in Rubona Ultisol and Nyamifumba Oxisol. Results revealed that travertine and wood ash increased the soil pH from 4.7 to 5.8 or higher and decreased exchangeable Al³⁺ and H⁺ to near 0 cmolc kg^?1. Soil nutrients generally increased to high or medium ranges for crop production. Leaf dry biomass, plant height and maize grain yields were significantly higher in Rubona Ultisol than in Nyamifumba Oxisol. Plots that received wood ash, with NPKS or with NPK, generally had higher maize yields, followed by those which received travertines and NPKS or NPK which had maize growth response as compared to the control plots which received NPK only. Thereby, a combination of wood ash with NPKS or NPK, travertines with NPKS was found to neutralize soil aluminum toxicity, increase soil nutrients status to required levels for plant growth and increase maize yields significantly.     

Effect of Raw and NH4+-enriched Zeolite on Nitrogen Uptake by Wheat and Nitrogen Leaching in Soils with Different Textures

Leaching of nutrients in soil can change the surface and groundwater quality. The present study aimed at investigating the effects of raw and ammonium (NH₄⁺)-enriched zeolite on nitrogen leaching and wheat yields in sandy loam and clay loam soils. The treatments were one level of nitrogen; Z₀: (100 kg (N) ha^?1) as urea, two levels of raw zeolite; Z₁:(0.5 g kg^?1 + 100 kg ha^?1) and Z_2: (1 g kg^?1 + 100 kg ha^?1), and two levels of NH₄⁺-enriched zeolite; Z₃: (0.5 g kg^?1 + 80 kg ha^?1) and Z₄: (1 g kg^?1 + 60 kg ha^?1). Wheat grains were sown in pots and, after each irrigation event, the leachates were collected and their nitrate (NO₃^?) and NH₄⁺ contents were determined. The grain yield and the total N in plants were measured after four months of wheat growth. The results indicated that the amounts of NH₄⁺ and NO₃^? leached from the sandy loam soil were more than those from the clay loam soil in all irrigation events. The maximum and minimum concentrations of nitrogen in the drainage water for both soils were observed at control and NH₄⁺-zeolite treatments, respectively. Total N in the plants grown in the sandy loam was higher compared to plants grown in clay loam soil. Also, nitrogen uptake by plants in control and NH₄⁺-zeolite was higher than that of raw-zeolite treatments. The decrease in the amount of N leaching in the presence of NH₄⁺-zeolite caused more N availability for plants and increased the efficiency of nitrogen fertilizers and the plants yield.     

Relationships between microbial biomass nitrogen,nitrate leaching and nitrogen uptake by corn in a compost and chemical fertilizer-amended regosol

Abstract

To determine the relationships between microbial biomass nitrogen (N), nitrate–nitrogen leaching (NO₃-N leaching) and N uptake by plants, a field experiment and a soil column experiment were conducted. In the field experiment, microbial biomass N, 0.5 mol L^?1 K₂SO₄ extractable N (extractable N), NO₃-N leaching and N uptake by corn were monitored in sawdust compost (SDC: 20 Mg ha^?1 containing 158 kg N ha^?1 of total N [approximately 50% is easily decomposable organic N]), chemical fertilizer (CF) and no fertilizer (NF) treatments from May 2000 to September 2002. In the soil column experiment, microbial biomass N, extractable N and NO₃-N leaching were monitored in soil treated with SDC (20 Mg ha^?1) + rice straw (RS) at five different application rates (0, 2.5, 5, 7.5 and 10 Mg ha^?1 containing 0, 15, 29, 44 and 59 kg N ha^?1) and in soil treated with CF in 2001. Nitrogen was applied as (NH₄)₂SO₄ at rates of 220 kg N ha^?1 for SDC and SDC + RS treatments and at a rate of 300 kg N ha^?1 for the CF treatment in both experiments. In the field experiment, microbial biomass N in the SDC treatment increased to 147 kg N ha^?1 at 7 days after treatment (DAT) and was maintained at 60–70 kg N ha^?1 after 30 days. Conversely, microbial biomass N in the CF treatment did not increase significantly. Extractable N in the surface soil increased immediately after treatment, but was found at lower levels in the SDC treatment compared to the CF treatment until 7 DAT. A small amount of NO₃-N leaching was observed until 21 DAT and increased markedly from 27 to 42 DAT in the SDC and CF treatments. Cumulative NO₃-N leaching in the CF treatment was 146 kg N ha^?1, which was equal to half of the applied N, but only 53 kg N ha^?1 in the SDC treatment. In contrast, there was no significant difference between N uptake by corn in the SDC and CF treatments. In the soil column experiment, microbial biomass N in the SDC + RS treatment at 7 DAT increased with increased RS application. Conversely, extractable N at 7 DAT and cumulative NO₃-N leaching until 42 DAT decreased with increased RS application. In both experiments, microbial biomass N was negatively correlated with extractable N at 7 DAT and cumulative NO₃-N leaching until 42 DAT, and extractable N was positively correlated with cumulative NO₃-N leaching. We concluded that microbial biomass N formation in the surface soil decreased extractable N and, consequently, contributed to decreasing NO₃-N leaching without impacting negatively on N uptake by plants.     

Nitrogen Balance in the Magruder Plots Following 109 Years in Continuous Winter Wheat

Abstract

The Magruder plots are the oldest continuous soil fertility wheat research plots in the Great Plains region, and are one of the oldest continuous soil fertility wheat plots in the world. They were initiated in 1892 by Alexander C. Magruder who was interested in the productivity of native prairie soils when sown continuously to winter wheat. This study reports on a simple estimate of nitrogen (N) balance in the Magruder plots, accounting for N applied, N removed in the grain, plant N loss, denitrification, non‐symbiotic N fixation, nitrate (NO₃ ^?) leaching, N applied in the rainfall, estimated total soil N (0–30 cm) at the beginning of the experiment and that measured in 2001. In the Manure plots, total soil N decreased from 6890 kg N ha^?1 in the surface 0–30 cm in 1892, to 3198 kg N ha^?1 in 2002. In the Check plots (no nutrients applied for 109 years) only 2411 kg N ha^?1 or 35% of the original total soil organic N remains. Nitrogen removed in the grain averaged 38.4 kg N ha^?1 yr^?1 and N additions (manure, N in rainfall, N via symbiotic N fixation) averaged 44.5 kg N ha^?1 yr^?1 in the Manure plots. Following 109 years, unaccounted N ranged from 229 to 1395 kg N ha^?1. On a by year basis, this would translate into 2–13 kg N ha^?1 yr^?1 that were unaccounted for, increasing with increased N application. For the Manure plots, the estimate of nitrogen use efficiency (NUE) (N removed in the grain, minus N removed in the grain of the Check plots, divided by the rate of N applied) was 32.8%, similar to the 33% NUE for world cereal production reported in 1999.     

Effects of application of lime nitrogen and dicyandiamide on nitrous oxide emissions from green tea fields

Abstract

The aim of this study was to assess the mitigating effects of lime nitrogen (calcium cyanamide) and dicyandiamide (DCD) application on nitrous oxide (N₂O) emissions from fields of green tea [Camellia sinensis (L.) Kuntze]. The study was conducted in experimental tea fields in which the fertilizer application rate was 544 kg nitrogen (N) ha^?1 yr^?1 for 2 years. The mean cumulative N₂O flux from the soil between the canopies of tea plants for 2 years was 7.1 ± 0.9 kg N ha^?1 yr^?1 in control plots. The cumulative N₂O flux in the plots supplemented with lime nitrogen was 3.5 ± 0.1 kgN ha^?1, approximately 51% lower than that in control plots. This reduction was due to the inhibition of nitrification by DCD, which was produced from the lime nitrogen. In addition, the increase in soil pH by lime in the lime nitrogen may also be another reason for the decreased N₂O emissions from soil in LN plots. Meanwhile, the cumulative N₂O flux in DCD plots was not significantly different from that in control plots. The seasonal variability in N₂O emissions in DCD plots differed from that in control plots and application of DCD sometimes increased N₂O emissions from tea field soil. The nitrification inhibition effect of lime nitrogen and DCD helped to delay nitrification of ammonium-nitrogen (NH₄⁺-N), leading to high NH₄⁺-N concentrations and a high ratio of NH₄⁺-N /nitrate-nitrogen (NO₃^–-N) in the soil. The inhibitors delayed the formation of NO₃^–-N in soil. N uptake by tea plants was almost the same among all three treatments.     

Effects of cotton (Gossypium hirsutum L.) straw and its biochar application on NH3 volatilization and N use efficiency in a drip-irrigated cotton field

Reducing ammonia (NH₃) volatilization is a practical way to increase nitrogen (N) fertilizer use efficiency (NUE). In this field study, soil was amended once with either cotton (Gossypium hirsutum L.) straw (6 t ha^?1) or its biochar (3.7 t ha^?1) unfertilized (0 kg N ha^?1) or fertilized (450 kg N ha^?1), and then soil inorganic N concentration and distribution, NH₃ volatilization, cotton yield and NUE were measured during the next two growing seasons. In unfertilized plots, NH₃ volatilization losses in the straw-amended and biochar-amended treatments were 38–40% and 42–46%, respectively, less than that in control (i.e., unamended soil) during the two growing seasons. In the fertilized plots, NH₃ volatilization losses in the straw-amended and biochar-amended treatments were 30–39% and 43–54%, respectively, less than that in the control. Straw amendment increased inorganic N concentrations, cotton yield, cotton N uptake and NUE during the first cropping season after application, but not during the second. In contrast, biochar increased cotton N uptake and NUE during both the first and the second cropping seasons after application. Furthermore, the effects of biochar on cotton N uptake and NUE were greater in the second year than in the first year. These results indicate that cotton straw and cotton straw biochar can both reduce NH₃ volatilization and also increase cotton yield, N uptake and NUE. In addition, the positive effects of one application of cotton straw biochar were more long-lasting than those of cotton straw.     

Ammonia volatilization following urea application at maize fields in the East African highlands with different soil properties

Use of nitrogen (N) fertilizer is underway to increase in Sub-Saharan Africa (SSA). The effect of increasing N rates on ammonia (NH₃) volatilization—a main pathway of applied-N loss in cropping systems—has not been evaluated in this region. In two soils (Alfisols, ALF; and Andisols, AND) with maize crop in the East African highlands, we measured NH₃ volatilization following urea broadcast at six rates (0–150 kg N ha^?1) for 17 days, using a semi-open static chamber method. Immediate irrigation and urea deep placement were tested as mitigation treatments. The underlying mechanism was assessed by monitoring soil pH and mineral N (NH₄⁺ and NO₃^?) concentrations. More cumulative NH₃-N was volatilized in ALF than in AND at the same urea-N rate. Generally, higher urea-N rates increased proportional NH₃-N loss (percent of applied N loss as NH₃-N). Based on well-fitted sigmoid models, simple surface urea application is not recommended for ALF, while up to 60 kg N ha^?1 could be adopted for AND soils. The susceptibility of ALF to NH₃ loss mainly resulted from its low pH buffering capacity, low cation exchange capacity, and high urease activity. Both mitigation treatments were effective. The inhibited rise of soil pH but not NH₄⁺ concentration was the main reason for the mitigated NH₃-N losses, although nitrification in the irrigation treatment might also have contributed. Our results showed that in acidic soils common to SSA croplands, proportional NH₃-N loss can be substantial even at a low urea-N rate; and that the design of mitigation treatments should consider the soil’s inherent capacity to buffer NH₃ loss.     

Die Rolle der mikrobiellen Biomasse und des mineralisch fixierten Ammoniums bei den Stickstoff-Transformationen in niedersächsischen Löß-Ackerböden unter Winter-Weizen. I. Poolgrößenveränderungen

Significance of microbial biomass and non-exchangeable ammonium with respect to the nitrogen transformations in loess soils of Niedersachsen during the growing season of winter wheat. I. Change of pool sizes Nitrogen transformations in loess soils have been examined by laboratory and field experiments. After straw application (· 8 t · ha^?1), N in microbial biomass (N_mic) increased by about 20 mg · kg^?1 soil (· 90 kg N · ha^?1 · 30 cm^?1) after 9 days of incubation (20 °C). Another laboratory experiment yielded an increase of about 400 mg of NH₄+-N · kg^?1 fixed by minerals within 1 h after addition of 1 M NH₄+-acetate. Defixation of the recently fixed NH₄+ after addition of 1 M KCl amounted to only 60 mg · kg^?1 within 50 days. In a field experiment with winter wheat 1991, an increase in N_mic of about 80 kg N · ha^?1 · 30 cm^?1 was observed from March to June. After July, growth of the microbes was limited by decreased soluble carbon concentrations in the rhizosphere. Different levels of mineral N-fertilizer (0, 177 and 213 kg N · ha^?1) did not affect significantly the microbial biomass. The same field experiment yielded a decrease of non-exchangeable ammonium on the “zero”-fertilized plot in spring by 200 kg N · ha^?1 · 30 cm^?1. The pool of fixed ammonium increased significantly after harvest. After conventional mineral N-fertilizer application (213 kg N · ha^?1). NH₄+-defixation was only about 120 kg N · ha^?1 · 30 cm^?1 until July.     

Using Wood Ash to Ameliorate Acidity and Improve Phosphorus Availability in Soils Amended with Partially Decomposed Cattle or Chicken Manure

The application of partially decomposed animal manure can acidify the soil by nitrification and may cause problems with phosphorus (P) availability. This study investigated the influence of applying wood ash to two soils amended with partially decomposed cattle or chicken manure on pH and P. The treatments consisted of two soils, a clay loam and sandy loam, each amended with partially decomposed chicken or cattle manure applied at 0, 5, or 15 t ha^?1, and wood ash was applied to each manure treatment at rates of 0 or 2 t ha^?1. The addition of wood ash significantly increased pH, thereby making more P available in soil and maize (Zea mays L.) tissues for both soils after being amended by manure. Both chicken and cattle manure significantly increased all the measured variables compared to the unamended soils. These results suggest that wood ash is an important amendment that could be used to amend partially decomposed manure, thereby not jeopardizing P availability to crops.     

Wood-ash fertilization and fire treatments in a Scots pine forest stand: Effects on the organic layer,microbial biomass,and microbial activity

We studied the reactions of humus layer (F/H) microbial respiratory activity, microbial biomass C, and the fungal biomass, measured as the soil ergosterol content, to the application of three levels of wood ash (1000, 2500, and 5000 kg ha^-1) and to fire treatment in a Scots pine (Pinus sylvestris L.) stand. Physicochemical measurements (pH, organic matter content, extractable and total C content, NH ₄ ⁺ and total N content, cation-exchange capacity, base saturation) showed similarity between the fire-treated plots and those treated with the lowest dose of wood ash (1000 kg ha^-1). The ash application did not change the level of microbial biomass C or fungal ergosterol when compared to the control, being around 7500 and 350 g g^-1 organic matter for the biomass C and ergosterol, respectively. The fire treatment lowered the values of both biomass measurements to about half that of the control values. The fire treatment caused a sevenfold fall in the respiration rate of fieldmoist soil to 1.8 l h^-1 g^-1 organic matter compared to the values of the control or ash treatments. However, in the same soils adjusted to a water-holding capacity of 60%, the differences between the fire treatment and the control were diminished, and the ash-fertilized plots were characterized by a higher respiration rate compared to the control plots. The glucose-induced respiration reacted in the same way as the water-adjusted soil respiration. The metabolic quotient, qCO₂, gradually increased from the control level with increasing applications of ash, reaching a maximum in the fire treatment. Nitrification was not observed in the treatment plots.     

Plant and Soil Responses to the Combined Application of Organic Amendments and Inorganic Fertilizers in Degraded Sodic Soils of Indo-Gangetic Plains

ABSTRACT

Soil degradation due to salinization and sodication is the paramount threat in Indo-Gangetic plains. The studies on reclamation and management of such soils can provide a pragmatic solution for improving fertility and productivity of these soils. Lack of organic matter and poor availability of nutrients are the major factors for low productivity of sodic soils. Rice-wheat is a major cropping system in Indo-Gangetic alluvial plain region even in reclaimed sodic soils and farmers used inorganic fertilizers only to get higher yields. In this study, we used different organic sources of amendments in conjunction with different nitrogen (N) doses supplied through inorganic fertilizers to investigate the combined effect of organic and inorganic amendments on soil fertility and the productivity of rice- wheat system in sodic soils. Salt tolerant varieties of rice and wheat were grown in sodic soil (pH: 9.30, EC: 1.12 dSm^?1 and exchangeable sodium percentage, ESP: 52) during 2014–15 to 2016–17 in a field experiment with 13 treatment combinations of organic and inorganic amendments (T₁- (control) 100% of recommended dose of N (RDN), T₂-municipal solid waste compost (MSWC) @10 t ha^?1 + 50%RDN, T₃- MSWC @10 t ha^?1 + 75% RDN,T₄- MSWC @10 t ha^?1 + 100%RDN, T₅-Vermicompost (VC) @10 t ha^?1 + 50% RDN, T₆- VC @10 t ha^?1 + 75% RDN, T₇-VC@10 t ha^?1 + 100% RDN, T₈- Farm yard manure (FYM) @ 10 t ha^?1 + 50% RDN,T₉- FYM@10 t ha^?1 + 75%RDN, T₁₀- FYM@10 t ha^?1 + 100% RDN, T₁₁-Pressmud (PM) @10 t ha^?1 + 50% RDN, T₁₂-PM@10 t ha^?1 + 75%RDN, and T₁₃- PM @ 10 t ha^?1 + 100% RDN). Use of organic amendments supplemented with reduced dose of N through inorganic fertilizer has significantly improved soil bio-physical and chemical properties. Application of VC@10 t ha^?1 + 100% RDN (T₇) decreased soil bulk density, pH, EC, ESP and Na content to 2.0, 4.2, 26.5, 42.8, and 56.6% respectively and increased soil organic carbon by 34.6% over control (T₁). Soil fertility in terms of available N, P, K, Ca, and Mg increased by 20.5, 33.0, 36.4, and 44%, respectively, over control (T₁). Soil microbial biomass carbon, nitrogen, and phosphorus also improved significantly due to combined use of organic amendments and inorganic fertilizers over the only use of inorganic fertilizers. Decreasing in soil sodicity and increasing soil fertility showed significant increase (P < 0.05) in crop growth, growth indices, and grain yields of rice and wheat. The study revealed that combined use of VC or MSW compost @10 t ha^?1 in conjunction with 75% RDN through inorganic fertilizers in sodic soils proved sustainable technology for restoration of degraded sodic soils and improving crop productivity.     

Long-Term Tillage,Straw Management,and Nitrogen Fertilization Effects on Organic Matter and Mineralizable Carbon and Nitrogen in a Black Chernozem Soil

Soil, crop, and fertilizer management practices may affect quality of organic carbon (C) and nitrogen (N) in soil. A long-term field experiment (growing barley, wheat, or canola)was conducted on a Black Chernozem (Albic Argicryoll) loam at Ellerslie, Alberta, Canada, to determine the influence of 19 years (1980 to 1998) of tillage [zero tillage (ZT) and conventional tillage (CT)], straw management [straw removed (S_Rem) and straw retained (S_Ret)], and N fertilizer rate (0, 50, and 100 kg N ha^?1 in S_Ret and 0 kg N ha^?1 in S_Rem plots) on macro-organic matter C (MOM-C) and N (MOM-N), microbial biomass C (MB-C), and mineralizable C (C_min) and N (N_min) in the 0- to 7.5-cm and 7.5- to 15-cm soil layers. Treatments with N fertilizer and S_Ret generally had a greater mass of MOM-C (by 201 kg C ha^?1 with 100 kg N ha^?1 rate and by 254 kg C ha^?1 with S_Ret), MOM-N (by 12.4 kg N ha^?1 with 100 kg N ha^?1 rate and by 8.0 kg N ha^?1 with S_Ret), C_min(by 146 kg C ha^?1 with 100 kg N ha^?1 rate and by 44 kg C ha^?1 with S_Ret), and N_min(by 7.9 kg N ha^?1 with 100 kg N ha^?1 rate and by 9.0 kg N ha^?1 with S_Ret)in soil than the corresponding zero-N and S_Rem treatments. Tillage, straw, and N fertilizer had no consistent effect on MB-C in soil. Correlations between these dynamic soil organic C or N fractions were strong and significant in most cases, except for MB-C, which had no significant correlation with MOM-C and MOM-N. Linear regressions between crop residue C input and mass of MOM-C, MOM-N, C_min, and N_min in soil were significant, but it was not significant for MB-C. The effects of management practices on dynamic soil organic C and N fractions were more pronounced in the 0- to 7.5-cm surface soil layer than in the 7.5- to 15-cm subsoil layer. In conclusion, the findings suggest that application of N fertilizer and retention of straw would improve soil quality by increasing macro-organic matter and N-supplying power of soil.     

Effects of liming and selected heavy metals on ammonium release in waterlogged agricultural soils

Many farmlands are periodically flooded or ponded by excessive precipitation resulting in changes to soil chemical and biochemical properties. In this study, one set (eight treatments with four replications) of field-moist surface soils (0–15 cm) and their air-dried counterparts obtained from a long-term liming experiment were incubated at 30 °C under waterlogged conditions for 10 days, and the amounts of net NH₄ ⁺-N released (soluble and exchangeable) were determined after extraction with 4 M KCl. Another set of three surface soils were used to evaluate the effect of six heavy metals on the NH₄ ⁺-N release under waterlogged conditions. Results showed that increasing the liming rate from 0 to 17,930 kg ha^?1 effective calcium carbonate equivalent increased the average soil pH from 4.98 to 7.06, averages of the amounts of NH₄ ⁺-N released ranged from 1.6 to 5.2 mg N kg^?1 field-moist soil, and the corresponding amounts released in air-dried soils ranged from 18.9 to 32.9 mg N kg^?1 soil. This increase of the amount NH₄ ⁺-N released in air-dried soil samples is presumably due to a slaking effect. At 5 mmol kg^?1 soil, all six heavy metals inhibited the NH₄ ⁺-N released. The relative effectiveness of the heavy metals in inhibition of the NH₄ ⁺-N released varied among the three soils. Lead(II) was the most effective inhibitor of NH ₄ ⁺-N release in Clarion and Harps soils and Cd(II) in Harps soil. Cobalt(II), Cu(II), and Cd(II) were the least effective inhibitors of NH₄ ⁺-N release in Clarion, Harps, and Okoboji soils, respectively.     

Coal Fly Ash and Phospho‐gypsum Mixture as an Amendment to Improve Rice Paddy Soil Fertility

Abstract

Rice is a plant that requires high levels of silica (Si). As a silicate (SiO₂) source to rice, coal fly ash (hereafter, fly ash), which has an alkaline pH and high available silicate and boron (B) contents, was mixed with phosphor‐gypsum (hereafter, gypsum, 50%, wt wt^?1), a by‐product from the production of phosphate fertilizer, to improve the fly ash limitation. Field experiments were carried out to evaluate the effect of the mixture on soil properties and rice (Oryza sativa) productivity in silt loam (SiL) and loamy sand (LS) soils to which 0 (FG 0), 20 (FG 20), 40 (FG 40), and 60 (FG 60) Mg ha^?1 were added. The mixture increased the amount of available silicate and exchangeable calcium (Ca) contents in the soils and the uptake of silicate by rice plant. The mixture did not result in accumulation of heavy metals in soil and an excessive uptake of heavy metals by the rice grain. The available boron content in soil increased with the mixture application levels up to 1.42 mg kg^?1 following the application of 60 Mg ha^?1 but did not show toxicity. The mixture increased significantly rice yield and showed the highest yields following the addition of 30–40 Mg ha^?1 in two soils. It is concluded that the fly ash and gypsum mixture could be a good source of inorganic soil amendments to restore the soil nutrient balance in rice paddy soil.     

Microbial ecology in tea soils

The soil chemical properties and microbial numbers in three volcanic ash soils and two non-volcanic ash soils, which had been continuously subjected to the same tea cultivation practices (21 y), were investigated. The results obtained were as follows. 1) pH values of all the soils gradually decreased from the original pH value (near neutral or mildly acid pH) to strongly acid values of about 4 or lower. In contrast, long-term tea cultivation practices resulted in the increase of the total C and N contents in the surface layers (0–20 cm) while the contents remained stable in the subsurface layers (20–40 cm). The increase in the organic matter content in non-volcanic ash soils was presumably due to the accumulation of microbial residues. The availability of P increased markedly. 2) Numbers of bacteria, actinomycetes, fungi, and denitrifiers were higher in volcanic ash soils than in non-volcanic ash soils, and also higher in surface layers than in subsurface layers. The results suggest that in spite of the same cultivation practices, the soil depth and soil type affected the microbial numbers in the tea soils. Numbers of autotrophic NH₄ ⁺ oxidizers were low in comparison with the numbers of autotrophic NO₂ ^- oxidizers. Influence of soil type and soil depth on autotrophic nitrifiers was not clear. 3) Total C and N contents in the tea soils were parameters closely related to the numbers of bacteria, actinomycetes, and fungi. For actinomycetes and fungi, the prediction could be more accurate, especially for total N content, if the estimations could be made within the same soil layers. The numbers per unit of C or N were higher in the surface layers than in the subsurface layers. 4) High concentration of NO₃ ^--N in the tea soils used suggests that nitrification could occur despite the low pH value (3.2-3.8). The negative relationship between the number of total bacteria or actinomycetes and soil NH⁴ ₊-N concentration suggests that some NH₄ ⁺-N was converted to organic microbial biomass-No.     

N2O and CH4 fluxes from a volcanic grassland soil in Nasu,Japan: Comparison between manure plus fertilizer plot and fertilizer-only plot

Abstract

We examined the effects of manure + fertilizer application and fertilizer-only application on nitrous oxide (N₂O) and methane (CH₄) fluxes from a volcanic grassland soil in Nasu, Japan. In the manure + fertilizer applied plot (manure plot), the sum of N mineralized from the manure and N applied as ammonium sulfate was adjusted to 210 kg N ha^?1 year^?1. In the fertilizer-only applied plot (fertilizer plot), 210 kg N ha^?1 year^?1 was applied as ammonium sulfate. The manure was applied to the manure plot in November and the fertilizer was applied to both plots in March, May, July and September. From November 2004 to November 2006, we regularly measured N₂O and CH₄ fluxes using closed chambers. Annual N₂O emissions from the manure and fertilizer plots ranged from 7.0 to 11.0 and from 4.7 to 9.1 kg N ha^?1, respectively. Annual N₂O emissions were greater from the manure plot than from the fertilizer plot (P < 0.05). This difference could be attributed to N₂O emissions following manure application. N₂O fluxes were correlated with soil temperature (R = 0.70, P < 0.001), NH⁺ ₄ concentration in the soil (R = 0.67, P < 0.001), soil pH (R = –0.46, P < 0.001) and NO^? ₃ concentration in the soil (R = 0.40, P < 0.001). When included in the multiple regression model (R = 0.72, P < 0.001), however, the following variables were significant: NH⁺ ₄ concentration in the soil (β = 0.52, P < 0.001), soil temperature (β = 0.36, P < 0.001) and soil moisture content (β = 0.26, P < 0.001). Annual CH₄ emissions from the manure and fertilizer plots ranged from –0.74 to –0.16 and from –0.84 to –0.52 kg C ha^?1, respectively. No significant difference was observed in annual CH₄ emissions between the plots. During the third grass-growing period from July to September, however, cumulative CH₄ emissions were greater from the manure plot than from the fertilizer plot (P < 0.05). CH₄ fluxes were correlated with NH⁺ ₄ concentration in the soil (R = 0.21, P < 0.05) and soil moisture content (R = 0.20, P < 0.05). When included in the multiple regression model (R = 0.29, P < 0.05), both NH⁺ ₄ concentration in the soil (β = 0.20, P < 0.05) and soil moisture content (β = 0.20, P < 0.05) were significant.     

Effect of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter on their productivity and N turnover in a Vertisol

A long-term field experiment was conducted for 8 years on a Vertisol in central India to assess quantitatively the direct and residual N effects of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter in a soybean–wheat rotation. After cultivation of soybean each year, its aerial residues were removed before growing wheat in the same plots using four N levels (120, 90, 60 and 30 kg ha^?1) and Azotobacter inoculation. Inoculation of soybean increased grain yield by 10.1% (180 kg ha^?1), but the increase in wheat yields with inoculation was only marginal (5.6%; 278 kg ha^?1). There was always a positive balance of soil N after soybean harvest; an average of +28 kg N ha^?1 yr^?1 in control (nodulated by native rhizobia) plots compared with +41 kg N ha^?1 yr^?1 in Rhizobium-inoculated plots. Residual and direct effects of Rhizobium and Azotobacter inoculants caused a fertilizer N credit of 30 kg ha^?1 in wheat. Application of fertilizers or microbial inoculation favoured the proliferation of rhizobia in crop rhizosphere due to better plant growth. Additional N uptake by inoculation was 14.9 kg N ha^?1 by soybean and 20.9 kg N ha^?1 by wheat crop, and a gain of +38.0 kg N ha^?1 yr^?1 to the 0–15 cm soil layer was measured after harvest of wheat. So, total N contribution to crops and soil due to the inoculants was 73.8 kg N ha^?1 yr^?1 after one soybean–wheat rotation. There was a total N benefit of 13.8 kg N ha^?1 yr^?1 to the soil due to regular long-term use of microbial inoculants in soybean–wheat rotation.     

Combined biochar and nitrogen fertilizer reduces soil acidity and promotes nutrient use efficiency by soybean crop

Purpose

Soil acidification is universal in soybean-growing fields. The aim of our research was to evaluate the effects of soil additives (N fertilizers and biochar) on crop performance and soil quality with specific emphasis on ameliorating soil acidity.

Materials and methods

Four nitrogen treatments were applied as follows: no nitrogen (N0), urea (N1), potassium nitrate (N2), and ammonium sulfate (N3), each providing 30 kg N ha^?1. Half plot area of the N1, N2, and N3 treatments was also treated with biochar (19.5 t ha^?1) to form N-biochar treatments (N1C, N2C, N3C). Both bulk and rhizosphere soils were sampled separately for the following analyses: pH, exchangeable base cations (EBC), exchangeable acidity (EA), total inorganic N (IN), total N (TN), and microbial phospholipid fatty acids (PLFAs). Soybean biomass and nutrient contents were also determined. Correlation analysis was applied to analyze the relationships between soil chemical properties and soybean plant parameters.

Results and discussion

With N-biochar additions (N1C, N2C, N3C), soil chemical properties changed as follows: pH increased by 0.6–1.2 units, EBC, IN, and TN increased by 175–419, 38.5–54.7, and 136–452 mg kg^?1, respectively, and PLFAs increased by 23.6–40.9 nmol g^?1 compared to the N0 in the rhizosphere. Microbial PLFAs had positive correlations with soil pH; EBC; exchangeable K, Ca, Na, and Mg; TN; IN; NH₄ ⁺; and NO₃ ^? (r?=?0.66–0.84, p?<?0.01). There were negative correlations between PLFAs and EA or exchangeable Al (r?=??0.64, ?0.66, p?<?0.01), which indicated that the additives increased microbial biomass by providing a suitable environment with less acid stress and more nutrients. The additives increased soil NH₄ ⁺ and NO₃ ^? by promoting soil organic N mineralization and reducing NH₄ ⁺ and NO₃ ^? leaching. Moreover, the soybean seed biomass and the nutrient contents in seeds increased with N-biochar additions, especially in the N3C treatment.

Conclusions

N-biochar additions were effective in ameliorating soil acidity, which improved the microenvironment for more microbial survival. N-biochars influenced N transformations at the plant–soil interface by increasing organic N mineralization, reducing N leaching, and promoting N uptake by soybeans. The soil additive ammonium and biochar (N3C) were best in promoting soybean growth.