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恶性纤维组织细胞瘤 (Malignant fibrous histo-cytoma,MFH)是动物的一种罕见的肿瘤 ,据文献记载 [1] ,主要发生于犬 ,其他动物未见报道 ,其分类不详。在人类 ,此肿瘤也不常见。一般根据其病理组织学形态不同 [2 ,3 ,4 ] 而将之分为以下几型 :席纹状多形型 (Storiform pleomorphic type) ;粘液型 (Myxoidtype)、巨细胞型 (Giant cell type) ;炎症型或恶性黄色肉芽肿 (Inflammatory type or Malignant xan-thogranuloma)和类血管型 (Angiomatoid type)。恶性纤维组织细胞瘤一般以上述分类的一种细胞形态或其中 2~ 3种细胞形态出现 ,而在… 相似文献
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猫皮肤肥大细胞瘤是猫第二常见的皮肤肿瘤,常发于中老年猫,病例数约占猫科动物皮肤瘤的20%,目前在我国文献中鲜有报道。本文介绍了近期临床接诊的一例猫肥大细胞瘤病例。患猫体表肿物位于右耳根部,呈粟粒样突起,无游离性;血常规及血液生化检查结果显示,患猫单核细胞数目、百分比下降,中性粒细胞百分比上升,球蛋白含量上升;H.E.染色可见病变位于表皮下真皮层内,病变细胞为卵圆形,呈弥漫性浸润性生长,排列成条索状或巢团状,细胞核呈圆形,胞质较丰富。综合临床检查、血常规检查、血液生化检查以及病理组织细胞学检查结果,体表肿物被诊断为猫肥大细胞瘤。经手术切除后,对患猫进行消炎抗菌、抗组胺以及肿瘤靶向药物治疗,1月后复检,患猫痊愈。该病例可为猫肥大细胞瘤的临床诊断与治疗提供参考。 相似文献
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一例肾脏恶性淋巴瘤患猫初诊时表现厌食、体重下降等非典型症状,血液学检查发现尿素氮与谷氨酸氨基转移酶偏低;B超扫查发现脾脏扭转、肝脏轻度肿大以及肾表面轻微不光滑。根据血液学及影像学检查结果初步诊断为肝脏疾病。但治疗3周后病情加重,再次血液学检查发现肌酐值偏高,白蛋白水平显著升高,尤其是B超扫查发现肾脏表面凸凹不平,根据肾脏组织病理学活检结果确诊为恶性淋巴瘤。该文讨论分析了该病例诊断过程。 相似文献
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PEI Yaping ZHAO Jin SUN Na SUN Panpan SUN Yaogui FAN Kuohai YIN Wei LI Hongquan 《畜牧兽医学报》1956,51(12):3068-3075
Ovarian granulosa cells provide a special microenvironment for follicle formation and maturation through interaction with oocytes and their own secretion. A variety of harmful stimuli can cause granulosa cell apoptosis and metabolic disorders, reduce the quality of oocytes and have a negative impact on embryo formation. Zearalenone (ZEA) is a common cause of ovarian granulosa cells injury in the livestock industry, which is produced by mycotoxins, and lack of effective treatment drug. Therefore, in the current study zearalenone was used to induce ovarian granulosa cell injury and to explore the protective effect of caffeic acid on zearalenone-induced ovarian granulosa cell apoptosis in mice. Mouse ovarian granulosa cells were isolated by mechanical method, and indirect immunofluorescence was used to identify the isolated cells. MTT assay was used to determine the effect of caffeic acid on the activity of normal mouse ovarian granulosa cells.After granulosa cells were co-treated with caffeic acid (200, 100 and 50 μg·mL-1) and ZEA for 24 hours, and control and ZEA group were set up at the same time, cell morphology and adherence were observed under a microscope. MTT was also used to detect cell viability. Caspase-3 mRNA expression level was detected by qRT-PCR. Cleaved-caspase-3 and cleaved-PARP protein expre-ssion levels were determined by Western blot. The results showed that positive FSHR staining appeared in cell cytoplasm of the test group, which confirmed that the isolated cells were mouse ovarian granulosa cells. The cell viability was above 90% which showed that caffeic acid had no toxic effect on granulosa cells. Compared with control group, ZEA group had smaller cell size, poor adherence, increased cell gap, and significant reduction in cell viability (P<0.001). Furthermore, the relative expression of caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein level were significantly increased (P<0.001) compared with the control group. After caffeic acid treatment, cell gap was reduced, adherence was tight, cell viability was significantly increased (P<0.001). Caffeic acid significantly reduced zearalenone-induced increase in caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein expression level (P<0.001). This study indicated that caffeic acid can restore granulosa cell viability by inhibiting ZEA-induced apoptosis. 相似文献
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卵巢颗粒细胞通过与卵母细胞相互作用及其自身分泌作用为卵泡的形成和发育成熟提供特殊的微环境。多种有害刺激可引起颗粒细胞凋亡和代谢失调从而降低卵母细胞质量,对胚胎的形成产生负面影响。玉米赤霉烯酮(zearalenone,ZEA)是畜禽养殖业中常见的造成卵巢颗粒细胞损伤的霉菌毒素,且缺少有效治疗药物。因此,本试验用ZEA造成小鼠卵巢颗粒细胞损伤,探究咖啡酸对ZEA诱导的小鼠卵巢颗粒细胞凋亡的保护作用。通过机械法分离小鼠卵巢颗粒细胞;运用间接免疫荧光法对分离出的细胞进行鉴定;使用MTT法测定咖啡酸对正常小鼠卵巢颗粒细胞活性的影响;选取200、100和50 μg·mL-1咖啡酸分别与ZEA共处理颗粒细胞,同时设置细胞对照组和ZEA模型组,24 h后显微镜观察细胞形态及贴壁情况,MTT法检测细胞活力;qRT-PCR技术检测caspase-3 mRNA的表达;Western blot检测cleaved-caspase-3和cleaved-PARP蛋白水平。结果发现,FSHR阳性染色大量出现在试验组细胞胞浆中,提示分离到的细胞是小鼠卵巢颗粒细胞;咖啡酸对卵巢颗粒细胞没有毒性作用且细胞活力均在90%以上;与空白对照组细胞相比,ZEA组细胞体积较小,贴壁较差,细胞间隙增大,细胞活力显著降低(P<0.001),caspase-3 mRNA相对表达量以及cleaved-caspase-3和cleaved-PARP蛋白表达水平显著升高(P<0.001),而给予咖啡酸处理后细胞间隙减小,贴壁紧密,细胞活力显著升高(P<0.001),显著降低ZEA诱导的caspase-3 mRNA含量增加(P<0.001),显著降低凋亡相关蛋白cleaved-caspase-3和cleaved-PARP的表达(P<0.001)。本研究发现,咖啡酸可通过抑制ZEA诱导的细胞凋亡,恢复颗粒细胞活性。 相似文献
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《Journal of Exotic Pet Medicine》2014,23(4):403-408
A 675-g, 1.4-year-old, female African pygmy hedgehog (Atelerix albiventris) was evaluated for progressive abdominal distension of 2 months duration. A large mass within the abdominal cavity was detected by radiography, ultrasonography, and computed tomography. The irregular, multilobulated mass, containing calcified, necrotic, and fatty areas was consistent with fused bilateral ovaries. Cytology of the mass suggested an ovarian adenoma or adenocarcinoma. The hedgehog died approximately 3 months after the initial presentation. Postmortem computed tomography and necropsy examination revealed a large bilaterally fused ovarian mass with peritoneal metastases. Histologically, the masses were composed of a variety of tissues from all 3 germ layers, including immature (embryonic) cells and neoplastic cells in the lymphatic system, which was consistent with malignant ovarian teratomas. This is the first report to describe malignant ovarian teratomas in a hedgehog. 相似文献
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Chih Chun WU Makoto NAKATA James Kenn CHAMBERS Kazuyuki UCHIDA Nobuo SASAKI Yasutsugu MIWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):685
A retrospective study involving eight African pygmy hedgehogs histopathologically diagnosed with granulosa cell tumors was conducted. The age at onset was 2.2–4.5 years, with a median age of 3.6 years. The most common clinical signs were hematuria and abdominal distension, which were observed in >50% cases. Exploratory laparotomy was performed in all cases, and ovariohysterectomy or excision of the abdominal mass was performed. Patients with only hematuria survived for >250 days after surgery, whereas those with initial ascites showed recurrence of ascites or tumor growth and survived for approximately 130 days after surgery. Intraperitoneal injection of carboplatin was performed in three recurrent cases. In one of these three cases, the tumor mass disappeared. Hence, carboplatin can be considered a potential antineoplastic drug for the treatment of granulosa cell tumors. 相似文献
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鹌鹑卵泡发育过程中颗粒细胞黄体生成素受体mRNA的表达 总被引:4,自引:0,他引:4
用Northern杂交的方法研究了鹌鹑排卵泡内颗粒细胞黄体生成素受体(LHR)mRNA的表达。在预计排卵前20h和3h分别取出最大的3个卵泡(F1、F3卵泡)以及小黄卵泡(SYF),剥离颗粒层提取出总RNA,并经变性凝胶电泳后将RNA转移到滤膜上。杂交所用的探针是用特异的引物经反转录一多聚栈链式反应(RT-PCR)扩增出编码LHRcDNA的细胞外区(EC)和跨膜区(TM)cDNA。结果表明,颗粒细胞LHRmR 相似文献
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研究主要是通过比较水牛卵巢颗粒细胞在不同培养基中的生长状态,并对细胞的增殖、核型及凋亡情况进行检测,以了解水牛卵巢颗粒细胞体外生长特性,建立水牛卵巢颗粒细胞的体外培养体系。结果发现,分离获得的水牛卵巢颗粒细胞存活率约为60%;采用DMEM培养基,颗粒细胞的生长速度和生长状态优于TCM-199和DMEM/F12培养基;细胞培养24 h后开始零星增殖,3~5 d增殖速度达到高峰;第1、3、5、7代颗粒细胞正常核型比率差异不显著,均在85%以上;第1代颗粒细胞的凋亡率与第5代差异显著(P<0.05),与第7代差异极显著(P<0.01)。结果表明,DMEM培养基更适宜用于水牛颗粒细胞的体外培养;水牛颗粒细胞能稳定地进行传代培养,染色体的数目不会发生明显改变,但细胞凋亡率会随着培养代数的增加而明显升高。 相似文献
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Faramarz Gharagozlou Reza Youssefi Vahid Akbarinejad Javad Ashrafihelan 《Journal of Equine Veterinary Science》2013
Anti-Müllerian hormone (AMH), produced by granulosa cells, has been reported to be elevated in mares with granulosa cell tumor. A 13-year-old Arabian mare was referred after 3 years of infertility. Rectal and ultrasonographic examination revealed enlargement and multicystic appearance of the left ovary. The mare had substantially higher concentration of AMH (21.6 ng/mL) compared with normal cyclic mares (n = 5; 0.36 ± 0.02 ng/mL). Testosterone (50 pg/mL) concentration was also elevated, but progesterone concentration (0.4 ng/mL) was lowered. Accordingly, the mare was presumptively diagnosed with granulosa-theca cell tumor and a left ovariectomy implemented using ventral midline approach under general anesthesia. Histopathological examination confirmed the diagnosis of granulosa-theca cell tumor. Serum concentrations of AMH decreased to 2 ng/mL and 0.5 ng/mL 2 and 4 weeks after tumor removal, respectively. In conclusion, the current report presents confirmatory evidence that evaluation of serum AMH could be useful for diagnosis of granulosa cell tumor in mare. 相似文献