Type 3 Diplococcus Pneumonia in Laboratory Rats

An acute respiratory disease, due to Diplococcus pneumoniae type 3, affecting Wistar and Sprague-Dawley rats is described. The infection was experimentally transmitted to Sprague-Dawley rats. The zoonotic potential of the condition dictated euthanasia of affected animals and instigation of rigid sanitary measures.     

兔肺炎双球菌病的病原分离鉴定及治疗

2013年山东滨州市某兔场发生仔兔高病死率、母兔流产的疾病。为了诊断治疗该病,本试验采集病死兔肺脏进行病原的分离,通过病原分离、形态学观察、生理生化试验、溶血试验、药敏试验等试验,证实分离菌为肺炎双球菌。该菌具有α-溶血性,对青霉素、头孢吡肟、阿米卡星等药物敏感;动物回归试验成功复制出兔肺炎双球菌病,症状典型,病理剖检变化明显。根据临床症状和药敏试验结果,用青霉素等药物进行治疗,病情得到有效控制。     

一例猪伪狂犬病野毒感染的实验室诊断

为确诊衡阳市某猪场育肥猪发生的疑似猪伪狂犬病(PR)疫情,采集发病猪脾脏,运用PCR方法进行猪伪狂犬病病毒(PRV)核酸检测,利用非洲绿猴肾细胞(Vero)进行病毒分离培养,采用免疫过氧化物酶单层细胞染色法(IPMA)进行病毒血清学鉴定;用分离培养物进行家兔感染试验,观察临床症状和病例剖检变化,采用PCR检测各器官中的病毒分布情况。结果显示:病料接种Vero细胞培养3 d后,出现明显的细胞病变效应(CPE);分离培养的病毒与PRV阳性血清呈阳性反应;家兔感染后出现奇痒等神经症状并死亡,其肝、脾组织均为PRV gE核酸检测阳性。试验结果证实,分离到的病毒为PRV野毒,从而确诊了该疑似疫情。     

四川省一些实验动物养殖场SPF大鼠和小鼠肠内容物中金黄色葡萄球菌的检测

调查四川省一些实验动物养殖场的实验动物中金黄色葡萄球菌(Staphylococcus aureus,SA)携带情况,找出传播途径和进化规律,分析其污染原因,为保障实验动物质量提供技术支撑。按照GB/T 14926.14-2001《实验动物金黄色葡萄球菌检测方法》对2011年-2017年四川省部分实验动物中SA进行检测,对分离的SA菌株进行PCR耐药基因mecA检测,并应用金黄色葡萄球菌A蛋白(Staphylococcus protein A,SPA)和脉冲场电泳(pulsed field gel electrophoresis,PFGE)进行分子分型。结果显示,2011年-2017年共从7家实验动物养殖场采集并完成370只实验动物中SA的检测,共检出SA 31株,总分离率为12.16%,且均从SPF大鼠中分离所得。31株SA的mecA基因均为阴性。SPA可分成5个型别,其中T2360为优势型别,PFGE型别有10个带型。四川省养殖实验动物中仅SPF大鼠携带SA,不同年份检出SA基因型别基本不同,不同养殖场同一年检出的SA具有相同基因型,说明不同养殖场中的SA可能来自同一污染源,应加强SPF大鼠监控,以确保实验动物的质量。     

2型糖尿病及并发肾病模型大鼠肠道菌群多样性分析

为分析2型糖尿病及并发肾病大鼠模型肠道菌群失衡模式及其与代谢和炎症相关指标之间的关系,通过高糖高脂饲料喂养联合腹腔注射链脲佐菌素法构建2型糖尿病大鼠模型,用相同方法联合单肾切除构建糖尿病肾病大鼠模型.成模后采集血液、尿液标本用于检测分析相关代谢及炎症水平变化,采集结肠粪便标本进行高通量测序分析.结果表明,与对照组相比,...     

Subclinical pneumonia in lambs

Groups of lambs were either slaughtered at weekly intervals between November and June, or regularly sampled over this period. The number of isolations of Pasteurella haemolytica and Mycoplasma sp. from the lungs increased during the later part of two seasons, as did the incidence of lesions typical of enzootic pneumonia. P. haemolytica was frequently isolated after February from nasal swabs in spite of the presence of serum antibody titres against this organism. Serum antibody titre to two adenoviruses were found to fall to a low level, one in January, the other in May, and then to rise again. Virus isolations were made at the time the homologous antibody titres were lowest.     

Rapid Identification of Mycoplasma pulmonis Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

Mycoplasma species identification is based on biochemical, immunological, and molecular methods that require several days for accurate identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a novel method for identification of bacteria and has recently been introduced into the clinical microbiology laboratory as a rapid and accurate technique. This method allows a characteristic mass spectral fingerprint to be obtained from whole inactivated mycoplasmal cells. In this study, we evaluated the performance of the MALDI-TOF MS for the identification of Mycoplasma by comparison with standard sequence analysis of 16S rRNA. We developed the first database of MALDI-TOF MS profiles of Mycoplasma species, containing Mycoplasma pulmonis, M. arthritidis, and M. neurolyticum, which are the most common pathogens in mice and/or rats, and species-specific spectra were recorded. Using the database, 6 clinical isolates were identified. Six tracheal swabs from 4 mice and 2 rats were cultured on PPLO agar for 4 to 7 days, and the colonies were directly applied to analyze the protein profiles. Five strains were identified as M. pulmonis, and 1 strain from a mouse was identified as M. neurolyticum (spectral scores were >2.00); the results were consistent with the results of the 16S rRNA gene sequence analysis (homologies>97.0%). These data indicate that MALDI-TOF MS can be used as a clearly rapid, accurate, and cost-effective method for the identification of M. pulmonis isolates, and this system may represent a serious alternative for clinical laboratories to identify Mycoplasma species.     

猪瘟病毒、猪圆环病毒2型、猪附红细胞体混合感染病例的实验室诊断

为确诊贵州省兴义市某养殖场生猪的发病原因,分别采集病死猪组织样品进行猪繁殖与呼吸综合征病毒、猪瘟病毒、猪圆环病毒2型、猪伪狂犬病病毒核酸检测,以及病猪耳静脉血进行血液原虫检查.结果:(1)实时荧光定量RT-PCR检测猪瘟病毒核酸和PCR检测猪圆环病毒2型核酸均为阳性.其余病毒核酸检测均为阴性.(2)血涂片姬姆萨染色观察...