共查询到20条相似文献,搜索用时 0 毫秒
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通过新疆金沟河弯道式引水枢纽改建工程模型试验研究,提出了在弯道内增设中导流堤和溢流侧堰的工程措施,较好地解决了第一代弯道式引水枢纽弯道过宽而产生的泥沙淤积和引水等问题,对于弯道式引水枢纽的改建和设计具有参考作用。 相似文献
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Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States 总被引:1,自引:0,他引:1
Lanciotti RS Roehrig JT Deubel V Smith J Parker M Steele K Crise B Volpe KE Crabtree MB Scherret JH Hall RA MacKenzie JS Cropp CB Panigrahy B Ostlund E Schmitt B Malkinson M Banet C Weissman J Komar N Savage HM Stone W McNamara T Gubler DJ 《Science (New York, N.Y.)》1999,286(5448):2333-2337
In late summer 1999, an outbreak of human encephalitis occurred in the northeastern United States that was concurrent with extensive mortality in crows (Corvus species) as well as the deaths of several exotic birds at a zoological park in the same area. Complete genome sequencing of a flavivirus isolated from the brain of a dead Chilean flamingo (Phoenicopterus chilensis), together with partial sequence analysis of envelope glycoprotein (E-glycoprotein) genes amplified from several other species including mosquitoes and two fatal human cases, revealed that West Nile (WN) virus circulated in natural transmission cycles and was responsible for the human disease. Antigenic mapping with E-glycoprotein-specific monoclonal antibodies and E-glycoprotein phylogenetic analysis confirmed these viruses as WN. This North American WN virus was most closely related to a WN virus isolated from a dead goose in Israel in 1998. 相似文献
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《淮南子》对国家空间的想象立足于大美的视野,以"中央"为国家空间想象的核心,并承载浓厚的人文色彩,将空间的自然秩序与人文秩序相统一,以满足大汉时代大一统社会文化需要。这样的大美空间在《淮南子》中无限广大、美不胜收,呈现出大道感性之美。《淮南子》以远古大道之初为想象性的大美时间,赋予其终极意义与最高生命价值,用来帮助人从现实中超越出来,实现一种自由、纯粹的生命理想。《淮南子》通过一种超越性的时间设计来应对当下,去寻求一种社会之道的理想图景;同时又明确历史现实存在的必然,理性看待历史发展过程中人文"事"迹。《淮南子》关于大美时间的远古想象,是一种文化理想建构的叙事策略。 相似文献
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吴燕 《山东省农业管理干部学院学报》2009,25(1):111-113
英国的陪审制度在世界司法制度史上占有重要地位。但关于英国陪审制度的起源,却一直是法制史中长期争论的话题,至今仍无确切答案。因此本文以着重介绍其起源为目的,并在此基础上简单探讨陪审制度在英国的确立及其原因。 相似文献
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易永姣 《湖南农业大学学报(自然科学版)》2013,(3):92-96
在中国古代文学作品中有许多人遇骷髅的故事,其骷髅意象明显经历了从先秦魏晋散文所展示的道家至乐逍遥思想,唐宋以后宗教视域下的空幻机巧,以及世情理念下的怪异、教化性特征等几次大的演变。骷髅意象之嬗变,与道家、宗教发展有着密切的联系,是特定时代社会背景下作者心理特质的反映。 相似文献
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The Galactic plane is a strong emitter of hard x-rays (2 to 10 kiloelectron volts), and the emission forms a narrow continuous ridge. The currently known hard x-ray sources are far too few to explain the ridge x-ray emission, and the fundamental question of whether the ridge emission is ultimately resolved into numerous dimmer discrete sources or truly diffuse emission has not yet been settled. In order to obtain a decisive answer, using the Chandra X-ray Observatory, we carried out the deepest hard x-ray survey of a Galactic plane region that is devoid of known x-ray point sources. We detected at least 36 new hard x-ray point sources in addition to strong diffuse emission within a 17' by 17' field of view. The surface density of the point sources is comparable to that at high Galactic latitudes after the effects of Galactic absorption are considered. Therefore, most of these point sources are probably extragalactic, presumably active galaxies seen through the Galactic disk. The Galactic ridge hard x-ray emission is diffuse, which indicates omnipresence within the Galactic plane of a hot plasma, the energy density of which is more than one order of magnitude higher than any other substance in the interstellar space. 相似文献
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从历史文献考证和香榧Torreya grandis `Merrillii' 古树调查资料分析得出香榧是榧树Torreya grandis 中的优良类型(单株)经无性繁殖培育而成的榧树优良品种。香榧起源于唐代,扩大栽培于宋代, 元、明、清三代得到规模发展。由于会稽山区榧树的应用历史悠久和社会经济条件使香榧得以保护和发展起来, 所以是香榧的原产地域。香榧以其特有的形态特征,优良的品质和原产地域而区别于一般榧树及其他变异类型。香榧是优良品种和著名的产品品牌, 除正宗香榧外, 其他品种类型均不能称为香榧。图1 表1 参20 相似文献
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Said R 《Science (New York, N.Y.)》1951,113(2940):517-518
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以弯形渠道为研究对象,结合实际工程,对带陡坡弯形渠道的水流偏斜特征进行研究,给出了3种不同形状和尺寸的渠道对同样流量工况下的水流偏斜特性的分析比较,可为合理有效地设计河流取水工程、航道与河道整治等类似的工程设计和研究提供参考。 相似文献
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陕京输气管道弯管力学性能 总被引:1,自引:0,他引:1
具有90年代国际先进水平的陕京输气管道工程,由于国内弯管标准对力学性能等没有要求或要求不明确,国外弯管标准不完全适用,因此参照国外普遍采用的先进标准及国内外制造弯管的经验,制定了该工程的弯管技术条件,力学性能等指标,这些指标均达到美国弯管标准。对实际制造的弯管检测数据表明,弯管力学性能等指标达到了技术条件的要求,与原材料钢管相比,σs,σb均降低,σs降幅更大,故屈强比降低,延伸率提高,表明弯管在 相似文献
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用Stratagene技术构建了吉富品系尼罗罗非鱼(Oreochromis niloticus,GIFT strain)肝cDNA文库。首先用TRIZOL法提取肝总RNA,经琼脂糖凝胶电泳和紫外分光光度检测,表明总RNA纯度高且完整性良好。然后分离纯化mRNA,合成双链cDNA之后,加EcoRⅠ接头、EcoRⅠ末端磷酸化、XhoⅠ消化,并用葡聚糖凝胶柱层析分级收集cDNA样品,得到300 mg cDNA,符合建库要求。双链cDNA与Uni-ZAP XR Vector连接后,用GigapackⅢGold Packaging Extract进行体外包装得到cDNA文库。测得的文库滴度为1.25×107pfu/mL,文库噬菌体的滴度符合文库保存和筛选实验的要求。随机挑选20个阳性单克隆噬菌斑进行PCR鉴定后,得出文库的重组率达100%,扩增出的片段主要集中在0.5~2 kb之间,平均插入片段长度约为1.05 kb,结果说明本实验所构建的cDNA文库质量较高。取扩增文库80μL进行体外切割,约含3.6×106个重组子,切割后phagemid量为2.0×106,切割效率为79.4%。经抽提质粒获得质粒DNA约1.2 mg。 相似文献
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尼罗罗非鱼肝cDNA文库的构建 总被引:3,自引:0,他引:3
用Stratagene技术构建了吉富品系尼罗罗非鱼(Oreochromis niloticus,GIFT strain)肝cDNA文库。首先用TRIZOL法提取肝总RNA,经琼脂糖凝胶电泳和紫外分光光度检测,表明总RNA纯度高且完整性良好。然后分离纯化mRNA,合成双链cDNA之后,加EcoRⅠ接头、EcoRⅠ末端磷酸化、XhoⅠ消化,并用葡聚糖凝胶柱层析分级收集cDNA样品,得到300 mg cDNA,符合建库要求。双链cDNA与Uni-ZAP XR Vector连接后,用GigapackⅢGold Packaging Extract进行体外包装得到cDNA文库。测得的文库滴度为1.25×107pfu/mL,文库噬菌体的滴度符合文库保存和筛选实验的要求。随机挑选20个阳性单克隆噬菌斑进行PCR鉴定后,得出文库的重组率达100%,扩增出的片段主要集中在0.5~2 kb之间,平均插入片段长度约为1.05 kb,结果说明本实验所构建的cDNA文库质量较高。取扩增文库80μL进行体外切割,约含3.6×106个重组子,切割后phagemid量为2.0×106,切割效率为79.4%。经抽提质粒获得质粒DNA约1.2 mg。 相似文献
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Enserink M 《Science (New York, N.Y.)》2000,290(5496):1482-1484
Fifteen months after the 1999 outbreak of West Nile virus in New York City, which sickened 62 mostly elderly people and killed seven, scientists are still hard pressed to predict how abundant the virus will eventually become or how serious a public health threat it will pose. This summer, the human toll has been relatively mild, with just 18 cases and one death. But the virus has been found in more than 60 bird species and about a dozen mammals; in a little more than a year, it has spread to 11 states along the East Coast and the District of Columbia. And with no natural barriers to stop it, scientists can safely say that it will keep spreading. 相似文献